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Supplemental Information

Screen Description

Classic HTS2

Classics / Classic HTS2
Created 2009-11-16.

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Classic HTS2

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Classic HTS2

    Screen Structure

    Classic HTS2

    Well Count 96
    Number of distinct ingredients 31
    Internal diversity (score mean dist) 0.925

    Screen Composition

    More information about screen composition chart.
    2025-06-14T13:15:45.279986 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Classic HTS2
    Buffer 105
    Cryoprotectant 7
    Organic (non-volatile) 41
    Organic (volatile) 27
    Polymer 8
    Salt 98

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Classic HTS2 Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Ammonium sulfate 0.5 M -
    • Lithium sulfate 1 M -
    • tri-Sodium citrate 0.1 M -
    		NA
    333 μl of Lithium sulfate (3 M)
    166 μl of Ammonium sulfate (3 M)
    25 μl of tri-Sodium citrate (4 M)
    475 μl of water (100 v/v)
    A2
    • Ammonium sulfate 1 M -
    • Sodium acetate 0.1 M pH 4.6
    		pH 4.6
    200 μl of Ammonium sulfate (5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    700 μl of water (100 v/v)
    A3
    • Ammonium sulfate 1 M -
    • Polyethylene glycol 400 2 w/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    26 μl of Polyethylene glycol 400 (75 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    333 μl of Ammonium sulfate (3 M)
    540 μl of water (100 v/v)
    A4
    • Ammonium sulfate 1 M -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    333 μl of Ammonium sulfate (3 M)
    566 μl of water (100 v/v)
    A5
    • Ammonium sulfate 1.2 M -
    • 2-Propanol 3 w/v -
    • tri-Sodium citrate 0.05 M -
    		NA
    60 μl of 2-Propanol (50 w/v)
    400 μl of Ammonium sulfate (3 M)
    12 μl of tri-Sodium citrate (4 M)
    527 μl of water (100 v/v)
    A6
    • Ammonium sulfate 1.5 M -
    • Glycerol 15 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    500 μl of Ammonium sulfate (3 M)
    187 μl of Glycerol (80 w/v)
    212 μl of water (100 v/v)
    A7
    • Ammonium sulfate 1.6 M -
    • Lithium sulfate 1 M -
    		NA
    333 μl of Lithium sulfate (3 M)
    533 μl of Ammonium sulfate (3 M)
    133 μl of water (100 v/v)
    A8
    • Ammonium sulfate 1.6 M -
    • HEPES 0.1 M pH 7.5
    • Sodium chloride 0.2 M -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    40 μl of Sodium chloride (5 M)
    533 μl of Ammonium sulfate (3 M)
    326 μl of water (100 v/v)
    A9
    • Ammonium sulfate 1.6 M -
    • Polyethylene glycol 1,000 2 w/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    40 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    533 μl of Ammonium sulfate (3 M)
    326 μl of water (100 v/v)
    A10
    • Ammonium sulfate 1.8 M -
    • MES 0.1 M pH 6.5
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    600 μl of Ammonium sulfate (3 M)
    300 μl of water (100 v/v)
    A11
    • Ammonium sulfate 2 M -
    • Sodium chloride 2 M -
    		NA
    500 μl of Ammonium sulfate (4 M)
    400 μl of Sodium chloride (5 M)
    100 μl of water (100 v/v)
    A12
    • Ammonium sulfate 2 M -
    • Sodium acetate 0.1 M pH 4.6
    		pH 4.6
    666 μl of Ammonium sulfate (3 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    233 μl of water (100 v/v)
    B1
    • Ammonium sulfate 2 M -
    • Polyethylene glycol 400 5 w/v -
    • MES 0.1 M pH 6.5
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    66 μl of Polyethylene glycol 400 (75 w/v)
    666 μl of Ammonium sulfate (3 M)
    166 μl of water (100 v/v)
    B2
    • Ammonium sulfate 2 M -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    666 μl of Ammonium sulfate (3 M)
    233 μl of water (100 v/v)
    B3
    • Ammonium sulfate 2.2 M -
    • Glycerol 20 w/v -
    		NA
    733 μl of Ammonium sulfate (3 M)
    250 μl of Glycerol (80 w/v)
    16 μl of water (100 v/v)
    B4
    • Ammonium sulfate 2.4 M -
    • tri-Sodium citrate 0.1 M -
    		NA
    800 μl of Ammonium sulfate (3 M)
    25 μl of tri-Sodium citrate (4 M)
    175 μl of water (100 v/v)
    B5
    • Ammonium sulfate 3 M -
    • 2-methyl-2,4-pentanediol BCC 1 w/v -
    		NA
    11 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
    600 μl of Ammonium sulfate (5 M)
    388 μl of water (100 v/v)
    B6
    • Ammonium sulfate 3 M -
    • Glycerol 10 w/v -
    		NA
    750 μl of Ammonium sulfate (4 M)
    200 μl of Glycerol (50 w/v)
    50 μl of water (100 v/v)
    B7
    • Ammonium sulfate 3.5 M -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    700 μl of Ammonium sulfate (5 M)
    200 μl of water (100 v/v)
    B8
    • Ammonium sulfate 3.5 M -
    • MES 0.1 M pH 6.5
    • 2-methyl-2,4-pentanediol BCC 1 w/v -
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    11 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
    700 μl of Ammonium sulfate (5 M)
    188 μl of water (100 v/v)
    B9
    • 2-methyl-2,4-pentanediol BCC 10 w/v -
    • HEPES 0.1 M pH 7.5
    • tri-Sodium citrate 0.1 M -
    		pH 7.5
    111 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    25 μl of tri-Sodium citrate (4 M)
    763 μl of water (100 v/v)
    B10
    • 2-methyl-2,4-pentanediol BCC 12 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Magnesium chloride 0.05 M -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    133 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
    20 μl of Magnesium chloride (2.5 M)
    746 μl of water (100 v/v)
    B11
    • 2-methyl-2,4-pentanediol BCC 15 w/v -
    • Polyethylene glycol 4,000 5 w/v -
    • Imidazole 0.1 M pH 8
    		pH 8
    100 μl of Imidazole pH 8 (1 M)
    166 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
    100 μl of Polyethylene glycol 4,000 (50 w/v)
    633 μl of water (100 v/v)
    B12
    • 2-methyl-2,4-pentanediol BCC 15 w/v -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Ammonium acetate 0.2 M -
    		pH 5.6
    166 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
    25 μl of tri-Sodium citrate pH 5.6 (4 M)
    40 μl of Ammonium acetate (5 M)
    768 μl of water (100 v/v)
    C1
    • 2-methyl-2,4-pentanediol BCC 15 w/v -
    • HEPES 0.1 M pH 7.5
    • tri-Sodium citrate 0.2 M -
    		pH 7.5
    166 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    50 μl of tri-Sodium citrate (4 M)
    683 μl of water (100 v/v)
    C2
    • 2-methyl-2,4-pentanediol BCC 20 w/v -
    • HEPES 0.1 M pH 7.5
    • tri-Sodium citrate 0.1 M -
    		pH 7.5
    222 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    25 μl of tri-Sodium citrate (4 M)
    652 μl of water (100 v/v)
    C3
    • 2-methyl-2,4-pentanediol BCC 20 w/v -
    • Imidazole 0.1 M pH 8
    		pH 8
    100 μl of Imidazole pH 8 (1 M)
    222 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
    677 μl of water (100 v/v)
    C4
    • 2-methyl-2,4-pentanediol BCC 20 w/v -
    • Glycerol 4 w/v -
    • Sodium chloride 0.2 M -
    		NA
    200 μl of Sodium chloride (1 M)
    222 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
    50 μl of Glycerol (80 w/v)
    527 μl of water (100 v/v)
    C5
    • 2-methyl-2,4-pentanediol BCC 30 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • Calcium chloride 0.02 M -
    		pH 4.6
    333 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    4 μl of Calcium chloride (5 M)
    562 μl of water (100 v/v)
    C6
    • 2-methyl-2,4-pentanediol BCC 30 w/v -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Ammonium acetate 0.2 M -
    		pH 5.6
    333 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
    25 μl of tri-Sodium citrate pH 5.6 (4 M)
    40 μl of Ammonium acetate (5 M)
    601 μl of water (100 v/v)
    C7
    • 2-methyl-2,4-pentanediol BCC 30 w/v -
    • HEPES 0.1 M pH 7.5
    • Ammonium sulfate 0.5 M -
    		pH 7.5
    333 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    166 μl of Ammonium sulfate (3 M)
    400 μl of water (100 v/v)
    C8
    • 2-methyl-2,4-pentanediol BCC 30 w/v -
    • HEPES 0.1 M pH 7.5
    • tri-Sodium citrate 0.2 M -
    		pH 7.5
    333 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    50 μl of tri-Sodium citrate (4 M)
    516 μl of water (100 v/v)
    C9
    • 2-methyl-2,4-pentanediol BCC 30 w/v -
    • Polyethylene glycol 4,000 10 w/v -
    • Imidazole 0.1 M pH 8
    		pH 8
    100 μl of Imidazole pH 8 (1 M)
    333 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    366 μl of water (100 v/v)
    C10
    • 2-methyl-2,4-pentanediol BCC 30 w/v -
    • Ethanol 20 w/v -
    		NA
    400 μl of Ethanol (50 w/v)
    333 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
    266 μl of water (100 v/v)
    C11
    • 2-methyl-2,4-pentanediol BCC 35 w/v -
    • Imidazole 0.1 M pH 8
    		pH 8
    100 μl of Imidazole pH 8 (1 M)
    388 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
    511 μl of water (100 v/v)
    C12
    • 2-methyl-2,4-pentanediol BCC 40 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    444 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
    455 μl of water (100 v/v)
    D1
    • 2-methyl-2,4-pentanediol BCC 47 w/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    522 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    377 μl of water (100 v/v)
    D2
    • 2-methyl-2,4-pentanediol BCC 47 w/v -
    • 2-Methyl-2-propanol 2 w/v -
    		NA
    40 μl of 2-Methyl-2-propanol (50 w/v)
    522 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
    437 μl of water (100 v/v)
    D3
    • 2-methyl-2,4-pentanediol BCC 50 w/v -
    		NA
    555 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
    444 μl of water (100 v/v)
    D4
    • 2-methyl-2,4-pentanediol BCC 50 w/v -
    • 2-Propanol 20 w/v -
    • Sodium acetate 0.05 M -
    • Sodium chloride 0.05 M -
    		NA
    400 μl of 2-Propanol (50 w/v)
    20 μl of Sodium acetate (2.5 M)
    555 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
    10 μl of Sodium chloride (5 M)
    14 μl of water (100 v/v)
    D5
    • 2-methyl-2,4-pentanediol BCC 50 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium di-hydrogen phosphate 0.1 M -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    555 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
    33 μl of Ammonium di-hydrogen phosphate (3 M)
    311 μl of water (100 v/v)
    D6
    • 2-methyl-2,4-pentanediol BCC 55 w/v -
    		NA
    611 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
    388 μl of water (100 v/v)
    D7
    • 2-methyl-2,4-pentanediol BCC 60 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • Calcium chloride 0.01 M -
    		pH 4.6
    666 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    2 μl of Calcium chloride (5 M)
    231 μl of water (100 v/v)
    D8
    • 2-methyl-2,4-pentanediol BCC 70 w/v -
    • MES 0.1 M pH 6.5
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    777 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
    122 μl of water (100 v/v)
    D9
    • 2-methyl-2,4-pentanediol BCC 70 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    777 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
    122 μl of water (100 v/v)
    D10
    • Methanol 20 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Calcium chloride 0.01 M -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Methanol (50 w/v)
    2 μl of Calcium chloride (5 M)
    498 μl of water (100 v/v)
    D11
    • Ethanol 2 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    40 μl of Ethanol (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    860 μl of water (100 v/v)
    D12
    • Ethanol 5 w/v -
    • 2-methyl-2,4-pentanediol BCC 5 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium chloride 0.2 M -
    		pH 8.5
    100 μl of Ethanol (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    55 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
    40 μl of Sodium chloride (5 M)
    704 μl of water (100 v/v)
    E1
    • Ethanol 10 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    200 μl of Ethanol (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    700 μl of water (100 v/v)
    E2
    • Ethanol 12 w/v -
    • Polyethylene glycol 400 4 w/v -
    • Sodium acetate 0.1 M pH 4.6
    		pH 4.6
    240 μl of Ethanol (50 w/v)
    53 μl of Polyethylene glycol 400 (75 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    606 μl of water (100 v/v)
    E3
    • Ethanol 14 w/v -
    • Glycerol 5 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    280 μl of Ethanol (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    62 μl of Glycerol (80 w/v)
    557 μl of water (100 v/v)
    E4
    • Ethanol 18 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    360 μl of Ethanol (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    540 μl of water (100 v/v)
    E5
    • Ethanol 20 w/v -
    • Glycerol 10 w/v -
    		NA
    400 μl of Ethanol (50 w/v)
    125 μl of Glycerol (80 w/v)
    475 μl of water (100 v/v)
    E6
    • Ethanol 30 w/v -
    • Polyethylene glycol 6,000 10 w/v -
    • Sodium acetate 0.1 M -
    		NA
    600 μl of Ethanol (50 w/v)
    40 μl of Sodium acetate (2.5 M)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    160 μl of water (100 v/v)
    E7
    • Ethanol 45 w/v -
    		NA
    900 μl of Ethanol (50 w/v)
    100 μl of water (100 v/v)
    E8
    • Ethanol 50 w/v -
    • Sodium acetate 0.01 M -
    		NA
    757 μl of Ethanol (66 w/v)
    4 μl of Sodium acetate (2.5 M)
    238 μl of water (100 v/v)
    E9
    • Ethanol 60 w/v -
    • Polyethylene glycol 6,000 1.5 w/v -
    • Sodium acetate 0.05 M -
    		NA
    909 μl of Ethanol (66 w/v)
    20 μl of Sodium acetate (2.5 M)
    30 μl of Polyethylene glycol 6,000 (50 w/v)
    40 μl of water (100 v/v)
    E10
    • Ethanol 60 w/v -
    • Sodium chloride 0.1 M -
    		NA
    909 μl of Ethanol (66 w/v)
    20 μl of Sodium chloride (5 M)
    70 μl of water (100 v/v)
    E11
    • 2-Propanol 5 w/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    100 μl of 2-Propanol (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    800 μl of water (100 v/v)
    E12
    • 2-Propanol 10 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • Calcium chloride 0.2 M -
    		pH 4.6
    200 μl of 2-Propanol (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    40 μl of Calcium chloride (5 M)
    660 μl of water (100 v/v)
    F1
    • 2-Propanol 10 w/v -
    • HEPES 0.1 M pH 7.5
    • tri-Sodium citrate 0.2 M -
    		pH 7.5
    200 μl of 2-Propanol (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    50 μl of tri-Sodium citrate (4 M)
    650 μl of water (100 v/v)
    F2
    • 2-Propanol 10 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Magnesium chloride 0.01 M -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    200 μl of 2-Propanol (50 w/v)
    4 μl of Magnesium chloride (2.5 M)
    696 μl of water (100 v/v)
    F3
    • 2-Propanol 15 w/v -
    • MES 0.1 M pH 6.5
    • tri-Sodium citrate 0.2 M -
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    300 μl of 2-Propanol (50 w/v)
    50 μl of tri-Sodium citrate (4 M)
    550 μl of water (100 v/v)
    F4
    • 2-Propanol 15 w/v -
    • HEPES 0.1 M pH 7.5
    • Magnesium chloride 0.2 M -
    		pH 7.5
    300 μl of 2-Propanol (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    80 μl of Magnesium chloride (2.5 M)
    520 μl of water (100 v/v)
    F5
    • 2-Propanol 15 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium acetate 0.2 M -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    300 μl of 2-Propanol (50 w/v)
    40 μl of Ammonium acetate (5 M)
    560 μl of water (100 v/v)
    F6
    • 2-Propanol 20 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • Calcium chloride 0.2 M -
    		pH 4.6
    400 μl of 2-Propanol (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    40 μl of Calcium chloride (5 M)
    460 μl of water (100 v/v)
    F7
    • 2-Propanol 25 w/v -
    • HEPES 0.1 M pH 7.5
    • Magnesium chloride 0.1 M -
    		pH 7.5
    500 μl of 2-Propanol (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    40 μl of Magnesium chloride (2.5 M)
    360 μl of water (100 v/v)
    F8
    • 2-Propanol 30 w/v -
    • MES 0.1 M pH 6.5
    • tri-Sodium citrate 0.2 M -
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    600 μl of 2-Propanol (50 w/v)
    50 μl of tri-Sodium citrate (4 M)
    250 μl of water (100 v/v)
    F9
    • 2-Propanol 30 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium acetate 0.2 M -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    600 μl of 2-Propanol (50 w/v)
    40 μl of Ammonium acetate (5 M)
    260 μl of water (100 v/v)
    F10
    • 2-Methyl-2-propanol 25 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Calcium chloride 0.1 M -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    500 μl of 2-Methyl-2-propanol (50 w/v)
    50 μl of Calcium chloride (2 M)
    350 μl of water (100 v/v)
    F11
    • 2-Methyl-2-propanol 35 w/v -
    • tri-Sodium citrate 0.1 M pH 5.6
    		pH 5.6
    700 μl of 2-Methyl-2-propanol (50 w/v)
    25 μl of tri-Sodium citrate pH 5.6 (4 M)
    275 μl of water (100 v/v)
    F12
    • Ammonium di-hydrogen phosphate 0.2 M -
    		NA
    66 μl of Ammonium di-hydrogen phosphate (3 M)
    933 μl of water (100 v/v)
    G1
    • Potassium Sodium tartrate 0.2 M -
    		NA
    200 μl of Potassium Sodium tartrate (1 M)
    800 μl of water (100 v/v)
    G2
    • Magnesium acetate 0.2 M -
    		NA
    100 μl of Magnesium acetate (2 M)
    900 μl of water (100 v/v)
    G3
    • Ammonium di-hydrogen phosphate 0.4 M -
    		NA
    133 μl of Ammonium di-hydrogen phosphate (3 M)
    866 μl of water (100 v/v)
    G4
    • Potassium Sodium tartrate 0.4 M -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Potassium Sodium tartrate (1 M)
    500 μl of water (100 v/v)
    G5
    • Ammonium di-hydrogen phosphate 0.5 M -
    • tri-Sodium citrate 0.2 M -
    		NA
    166 μl of Ammonium di-hydrogen phosphate (3 M)
    50 μl of tri-Sodium citrate (4 M)
    783 μl of water (100 v/v)
    G6
    • Sodium acetate 0.5 M -
    • Imidazole 0.1 M pH 8
    		pH 8
    100 μl of Imidazole pH 8 (1 M)
    200 μl of Sodium acetate (2.5 M)
    700 μl of water (100 v/v)
    G7
    • tri-Sodium citrate 0.7 M -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    175 μl of tri-Sodium citrate (4 M)
    725 μl of water (100 v/v)
    G8
    • Potassium Sodium tartrate 0.8 M -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    800 μl of Potassium Sodium tartrate (1 M)
    100 μl of water (100 v/v)
    G9
    • Ammonium di-hydrogen phosphate 1 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    		pH 5.6
    333 μl of Ammonium di-hydrogen phosphate (3 M)
    25 μl of tri-Sodium citrate pH 5.6 (4 M)
    641 μl of water (100 v/v)
    G10
    • Ammonium di-hydrogen phosphate 1 M -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    333 μl of Ammonium di-hydrogen phosphate (3 M)
    566 μl of water (100 v/v)
    G11
    • Lithium sulfate 1 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Nickel (II) chloride 0.01 M -
    		pH 8.5
    333 μl of Lithium sulfate (3 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    10 μl of Nickel (II) chloride (1 M)
    556 μl of water (100 v/v)
    G12
    • Sodium acetate 1 M -
    • Imidazole 0.1 M pH 8
    		pH 8
    100 μl of Imidazole pH 8 (1 M)
    400 μl of Sodium acetate (2.5 M)
    500 μl of water (100 v/v)
    H1
    • Sodium acetate 1.4 M -
    • MES 0.1 M pH 6.5
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    560 μl of Sodium acetate (2.5 M)
    340 μl of water (100 v/v)
    H2
    • Lithium sulfate 1.5 M -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    500 μl of Lithium sulfate (3 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of water (100 v/v)
    H3
    • tri-Sodium citrate 1.5 M -
    		NA
    750 μl of tri-Sodium citrate pH 6.5 (2 M)
    250 μl of water (100 v/v)
    H4
    • Magnesium sulfate 1.6 M -
    • MES 0.1 M pH 6.5
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    800 μl of Magnesium sulfate (2 M)
    100 μl of water (100 v/v)
    H5
    • Potassium Sodium tartrate 1.6 M -
    • MES 0.1 M pH 6.5
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    800 μl of Potassium Sodium tartrate (2 M)
    100 μl of water (100 v/v)
    H6
    • Ammonium formate 2 M -
    • MES 0.1 M pH 6.5
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    400 μl of Ammonium formate (5 M)
    500 μl of water (100 v/v)
    H7
    • Ammonium di-hydrogen phosphate 2 M -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    666 μl of Ammonium di-hydrogen phosphate (3 M)
    233 μl of water (100 v/v)
    H8
    • Sodium chloride 2 M -
    • MES 0.1 M pH 6.5
    • Sodium acetate 0.2 M -
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    80 μl of Sodium acetate (2.5 M)
    400 μl of Sodium chloride (5 M)
    420 μl of water (100 v/v)
    H9
    • Sodium formate 2 M -
    • Sodium acetate 0.1 M pH 4.6
    		pH 4.6
    285 μl of Sodium formate (7 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    614 μl of water (100 v/v)
    H10
    • Ammonium di-hydrogen phosphate 1 M -
    • Glycerol 30 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    333 μl of Ammonium di-hydrogen phosphate (3 M)
    375 μl of Glycerol (80 w/v)
    191 μl of water (100 v/v)
    H11
    • Sodium chloride 4 M -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    800 μl of Sodium chloride (5 M)
    100 μl of water (100 v/v)
    H12
    • Sodium formate 3 M -
    		NA
    428 μl of Sodium formate (7 M)
    571 μl of water (100 v/v)