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Supplemental Information

Screen Description

Classic HTS2

Classics / Classic HTS2
Created 2009-11-16.

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Classic HTS2

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Classic HTS2

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Screen Structure

Classic HTS2

Well Count 96
Number of distinct ingredients 31
Internal diversity (score mean dist) 0.925

Screen Composition

More information about screen composition chart.
2025-08-16T04:27:13.996441 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Classic HTS2
Buffer 105
Cryoprotectant 7
Organic (non-volatile) 41
Organic (volatile) 27
Polymer 8
Salt 98

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Classic HTS2 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Ammonium sulfate 0.5 M -
  • Lithium sulfate 1 M -
  • tri-Sodium citrate 0.1 M -
NA
166.7 μl of Ammonium sulfate (3 M)
333.3 μl of Lithium sulfate (3 M)
25.0 μl of tri-Sodium citrate (4 M)
475.0 μl of water (100 v/v)
A2
  • Ammonium sulfate 1 M -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
200.0 μl of Ammonium sulfate (5 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
700.0 μl of water (100 v/v)
A3
  • Ammonium sulfate 1 M -
  • Polyethylene glycol 400 2 w/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
333.3 μl of Ammonium sulfate (3 M)
100.0 μl of HEPES pH 7.5 (1 M)
26.7 μl of Polyethylene glycol 400 (75 w/v)
540.0 μl of water (100 v/v)
A4
  • Ammonium sulfate 1 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
333.3 μl of Ammonium sulfate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
566.7 μl of water (100 v/v)
A5
  • Ammonium sulfate 1.2 M -
  • 2-Propanol 3 w/v -
  • tri-Sodium citrate 0.05 M -
NA
60.0 μl of 2-Propanol (50 w/v)
400.0 μl of Ammonium sulfate (3 M)
12.5 μl of tri-Sodium citrate (4 M)
527.5 μl of water (100 v/v)
A6
  • Ammonium sulfate 1.5 M -
  • Glycerol 15 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
500.0 μl of Ammonium sulfate (3 M)
187.5 μl of Glycerol (80 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
212.5 μl of water (100 v/v)
A7
  • Ammonium sulfate 1.6 M -
  • Lithium sulfate 1 M -
NA
533.3 μl of Ammonium sulfate (3 M)
333.3 μl of Lithium sulfate (3 M)
133.3 μl of water (100 v/v)
A8
  • Ammonium sulfate 1.6 M -
  • HEPES 0.1 M pH 7.5
  • Sodium chloride 0.2 M -
pH 7.5
533.3 μl of Ammonium sulfate (3 M)
100.0 μl of HEPES pH 7.5 (1 M)
40.0 μl of Sodium chloride (5 M)
326.7 μl of water (100 v/v)
A9
  • Ammonium sulfate 1.6 M -
  • Polyethylene glycol 1,000 2 w/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
533.3 μl of Ammonium sulfate (3 M)
100.0 μl of HEPES pH 7.5 (1 M)
40.0 μl of Polyethylene glycol 1,000 (50 w/v)
326.7 μl of water (100 v/v)
A10
  • Ammonium sulfate 1.8 M -
  • MES 0.1 M pH 6.5
pH 6.5
600.0 μl of Ammonium sulfate (3 M)
100.0 μl of MES pH 6.5 (1 M)
300.0 μl of water (100 v/v)
A11
  • Ammonium sulfate 2 M -
  • Sodium chloride 2 M -
NA
500.0 μl of Ammonium sulfate (4 M)
400.0 μl of Sodium chloride (5 M)
100.0 μl of water (100 v/v)
A12
  • Ammonium sulfate 2 M -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
666.7 μl of Ammonium sulfate (3 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
233.3 μl of water (100 v/v)
B1
  • Ammonium sulfate 2 M -
  • Polyethylene glycol 400 5 w/v -
  • MES 0.1 M pH 6.5
pH 6.5
666.7 μl of Ammonium sulfate (3 M)
100.0 μl of MES pH 6.5 (1 M)
66.7 μl of Polyethylene glycol 400 (75 w/v)
166.7 μl of water (100 v/v)
B2
  • Ammonium sulfate 2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
666.7 μl of Ammonium sulfate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
233.3 μl of water (100 v/v)
B3
  • Ammonium sulfate 2.2 M -
  • Glycerol 20 w/v -
NA
733.3 μl of Ammonium sulfate (3 M)
250.0 μl of Glycerol (80 w/v)
16.7 μl of water (100 v/v)
B4
  • Ammonium sulfate 2.4 M -
  • tri-Sodium citrate 0.1 M -
NA
800.0 μl of Ammonium sulfate (3 M)
25.0 μl of tri-Sodium citrate (4 M)
175.0 μl of water (100 v/v)
B5
  • Ammonium sulfate 3 M -
  • 2-methyl-2,4-pentanediol BCC 1 w/v -
NA
11.1 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
600.0 μl of Ammonium sulfate (5 M)
388.9 μl of water (100 v/v)
B6
  • Ammonium sulfate 3 M -
  • Glycerol 10 w/v -
NA
750.0 μl of Ammonium sulfate (4 M)
200.0 μl of Glycerol (50 w/v)
50.0 μl of water (100 v/v)
B7
  • Ammonium sulfate 3.5 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
700.0 μl of Ammonium sulfate (5 M)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of water (100 v/v)
B8
  • Ammonium sulfate 3.5 M -
  • MES 0.1 M pH 6.5
  • 2-methyl-2,4-pentanediol BCC 1 w/v -
pH 6.5
11.1 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
700.0 μl of Ammonium sulfate (5 M)
100.0 μl of MES pH 6.5 (1 M)
188.9 μl of water (100 v/v)
B9
  • 2-methyl-2,4-pentanediol BCC 10 w/v -
  • HEPES 0.1 M pH 7.5
  • tri-Sodium citrate 0.1 M -
pH 7.5
111.1 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
100.0 μl of HEPES pH 7.5 (1 M)
25.0 μl of tri-Sodium citrate (4 M)
763.9 μl of water (100 v/v)
B10
  • 2-methyl-2,4-pentanediol BCC 12 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Magnesium chloride 0.05 M -
pH 8.5
133.3 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
20.0 μl of Magnesium chloride (2.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
746.7 μl of water (100 v/v)
B11
  • 2-methyl-2,4-pentanediol BCC 15 w/v -
  • Polyethylene glycol 4,000 5 w/v -
  • Imidazole 0.1 M pH 8
pH 8
166.7 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
100.0 μl of Imidazole pH 8 (1 M)
100.0 μl of Polyethylene glycol 4,000 (50 w/v)
633.3 μl of water (100 v/v)
B12
  • 2-methyl-2,4-pentanediol BCC 15 w/v -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Ammonium acetate 0.2 M -
pH 5.6
166.7 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
40.0 μl of Ammonium acetate (5 M)
25.0 μl of tri-Sodium citrate pH 5.6 (4 M)
768.3 μl of water (100 v/v)
C1
  • 2-methyl-2,4-pentanediol BCC 15 w/v -
  • HEPES 0.1 M pH 7.5
  • tri-Sodium citrate 0.2 M -
pH 7.5
166.7 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
100.0 μl of HEPES pH 7.5 (1 M)
50.0 μl of tri-Sodium citrate (4 M)
683.3 μl of water (100 v/v)
C2
  • 2-methyl-2,4-pentanediol BCC 20 w/v -
  • HEPES 0.1 M pH 7.5
  • tri-Sodium citrate 0.1 M -
pH 7.5
222.2 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
100.0 μl of HEPES pH 7.5 (1 M)
25.0 μl of tri-Sodium citrate (4 M)
652.8 μl of water (100 v/v)
C3
  • 2-methyl-2,4-pentanediol BCC 20 w/v -
  • Imidazole 0.1 M pH 8
pH 8
222.2 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
100.0 μl of Imidazole pH 8 (1 M)
677.8 μl of water (100 v/v)
C4
  • 2-methyl-2,4-pentanediol BCC 20 w/v -
  • Glycerol 4 w/v -
  • Sodium chloride 0.2 M -
NA
222.2 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
50.0 μl of Glycerol (80 w/v)
200.0 μl of Sodium chloride (1 M)
527.8 μl of water (100 v/v)
C5
  • 2-methyl-2,4-pentanediol BCC 30 w/v -
  • Sodium acetate 0.1 M pH 4.6
  • Calcium chloride 0.02 M -
pH 4.6
333.3 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
4.0 μl of Calcium chloride (5 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
562.7 μl of water (100 v/v)
C6
  • 2-methyl-2,4-pentanediol BCC 30 w/v -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Ammonium acetate 0.2 M -
pH 5.6
333.3 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
40.0 μl of Ammonium acetate (5 M)
25.0 μl of tri-Sodium citrate pH 5.6 (4 M)
601.7 μl of water (100 v/v)
C7
  • 2-methyl-2,4-pentanediol BCC 30 w/v -
  • HEPES 0.1 M pH 7.5
  • Ammonium sulfate 0.5 M -
pH 7.5
333.3 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
166.7 μl of Ammonium sulfate (3 M)
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of water (100 v/v)
C8
  • 2-methyl-2,4-pentanediol BCC 30 w/v -
  • HEPES 0.1 M pH 7.5
  • tri-Sodium citrate 0.2 M -
pH 7.5
333.3 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
100.0 μl of HEPES pH 7.5 (1 M)
50.0 μl of tri-Sodium citrate (4 M)
516.7 μl of water (100 v/v)
C9
  • 2-methyl-2,4-pentanediol BCC 30 w/v -
  • Polyethylene glycol 4,000 10 w/v -
  • Imidazole 0.1 M pH 8
pH 8
333.3 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
100.0 μl of Imidazole pH 8 (1 M)
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
366.7 μl of water (100 v/v)
C10
  • 2-methyl-2,4-pentanediol BCC 30 w/v -
  • Ethanol 20 w/v -
NA
333.3 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
400.0 μl of Ethanol (50 w/v)
266.7 μl of water (100 v/v)
C11
  • 2-methyl-2,4-pentanediol BCC 35 w/v -
  • Imidazole 0.1 M pH 8
pH 8
388.9 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
100.0 μl of Imidazole pH 8 (1 M)
511.1 μl of water (100 v/v)
C12
  • 2-methyl-2,4-pentanediol BCC 40 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
444.4 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
455.6 μl of water (100 v/v)
D1
  • 2-methyl-2,4-pentanediol BCC 47 w/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
522.2 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
100.0 μl of HEPES pH 7.5 (1 M)
377.8 μl of water (100 v/v)
D2
  • 2-methyl-2,4-pentanediol BCC 47 w/v -
  • 2-Methyl-2-propanol 2 w/v -
NA
40.0 μl of 2-Methyl-2-propanol (50 w/v)
522.2 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
437.8 μl of water (100 v/v)
D3
  • 2-methyl-2,4-pentanediol BCC 50 w/v -
NA
555.6 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
444.4 μl of water (100 v/v)
D4
  • 2-methyl-2,4-pentanediol BCC 50 w/v -
  • 2-Propanol 20 w/v -
  • Sodium acetate 0.05 M -
  • Sodium chloride 0.05 M -
NA
400.0 μl of 2-Propanol (50 w/v)
555.6 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
20.0 μl of Sodium acetate (2.5 M)
10.0 μl of Sodium chloride (5 M)
14.4 μl of water (100 v/v)
D5
  • 2-methyl-2,4-pentanediol BCC 50 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium di-hydrogen phosphate 0.1 M -
pH 8.5
555.6 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
33.3 μl of Ammonium di-hydrogen phosphate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
311.1 μl of water (100 v/v)
D6
  • 2-methyl-2,4-pentanediol BCC 55 w/v -
NA
611.1 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
388.9 μl of water (100 v/v)
D7
  • 2-methyl-2,4-pentanediol BCC 60 w/v -
  • Sodium acetate 0.1 M pH 4.6
  • Calcium chloride 0.01 M -
pH 4.6
666.7 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
2.0 μl of Calcium chloride (5 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
231.3 μl of water (100 v/v)
D8
  • 2-methyl-2,4-pentanediol BCC 70 w/v -
  • MES 0.1 M pH 6.5
pH 6.5
777.8 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
100.0 μl of MES pH 6.5 (1 M)
122.2 μl of water (100 v/v)
D9
  • 2-methyl-2,4-pentanediol BCC 70 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
777.8 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
122.2 μl of water (100 v/v)
D10
  • Methanol 20 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Calcium chloride 0.01 M -
pH 8.5
2.0 μl of Calcium chloride (5 M)
400.0 μl of Methanol (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
498.0 μl of water (100 v/v)
D11
  • Ethanol 2 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
40.0 μl of Ethanol (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
860.0 μl of water (100 v/v)
D12
  • Ethanol 5 w/v -
  • 2-methyl-2,4-pentanediol BCC 5 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Sodium chloride 0.2 M -
pH 8.5
55.6 μl of 2-methyl-2,4-pentanediol BCC (90 w/v)
100.0 μl of Ethanol (50 w/v)
40.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
704.4 μl of water (100 v/v)
E1
  • Ethanol 10 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
200.0 μl of Ethanol (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
700.0 μl of water (100 v/v)
E2
  • Ethanol 12 w/v -
  • Polyethylene glycol 400 4 w/v -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
240.0 μl of Ethanol (50 w/v)
53.3 μl of Polyethylene glycol 400 (75 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
606.7 μl of water (100 v/v)
E3
  • Ethanol 14 w/v -
  • Glycerol 5 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
280.0 μl of Ethanol (50 w/v)
62.5 μl of Glycerol (80 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
557.5 μl of water (100 v/v)
E4
  • Ethanol 18 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
360.0 μl of Ethanol (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
540.0 μl of water (100 v/v)
E5
  • Ethanol 20 w/v -
  • Glycerol 10 w/v -
NA
400.0 μl of Ethanol (50 w/v)
125.0 μl of Glycerol (80 w/v)
475.0 μl of water (100 v/v)
E6
  • Ethanol 30 w/v -
  • Polyethylene glycol 6,000 10 w/v -
  • Sodium acetate 0.1 M -
NA
600.0 μl of Ethanol (50 w/v)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
40.0 μl of Sodium acetate (2.5 M)
160.0 μl of water (100 v/v)
E7
  • Ethanol 45 w/v -
NA
900.0 μl of Ethanol (50 w/v)
100.0 μl of water (100 v/v)
E8
  • Ethanol 50 w/v -
  • Sodium acetate 0.01 M -
NA
757.6 μl of Ethanol (66 w/v)
4.0 μl of Sodium acetate (2.5 M)
238.4 μl of water (100 v/v)
E9
  • Ethanol 60 w/v -
  • Polyethylene glycol 6,000 1.5 w/v -
  • Sodium acetate 0.05 M -
NA
909.1 μl of Ethanol (66 w/v)
30.0 μl of Polyethylene glycol 6,000 (50 w/v)
20.0 μl of Sodium acetate (2.5 M)
40.9 μl of water (100 v/v)
E10
  • Ethanol 60 w/v -
  • Sodium chloride 0.1 M -
NA
909.1 μl of Ethanol (66 w/v)
20.0 μl of Sodium chloride (5 M)
70.9 μl of water (100 v/v)
E11
  • 2-Propanol 5 w/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of 2-Propanol (50 w/v)
100.0 μl of HEPES pH 7.5 (1 M)
800.0 μl of water (100 v/v)
E12
  • 2-Propanol 10 w/v -
  • Sodium acetate 0.1 M pH 4.6
  • Calcium chloride 0.2 M -
pH 4.6
200.0 μl of 2-Propanol (50 w/v)
40.0 μl of Calcium chloride (5 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
660.0 μl of water (100 v/v)
F1
  • 2-Propanol 10 w/v -
  • HEPES 0.1 M pH 7.5
  • tri-Sodium citrate 0.2 M -
pH 7.5
200.0 μl of 2-Propanol (50 w/v)
100.0 μl of HEPES pH 7.5 (1 M)
50.0 μl of tri-Sodium citrate (4 M)
650.0 μl of water (100 v/v)
F2
  • 2-Propanol 10 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Magnesium chloride 0.01 M -
pH 8.5
200.0 μl of 2-Propanol (50 w/v)
4.0 μl of Magnesium chloride (2.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
696.0 μl of water (100 v/v)
F3
  • 2-Propanol 15 w/v -
  • MES 0.1 M pH 6.5
  • tri-Sodium citrate 0.2 M -
pH 6.5
300.0 μl of 2-Propanol (50 w/v)
100.0 μl of MES pH 6.5 (1 M)
50.0 μl of tri-Sodium citrate (4 M)
550.0 μl of water (100 v/v)
F4
  • 2-Propanol 15 w/v -
  • HEPES 0.1 M pH 7.5
  • Magnesium chloride 0.2 M -
pH 7.5
300.0 μl of 2-Propanol (50 w/v)
100.0 μl of HEPES pH 7.5 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
520.0 μl of water (100 v/v)
F5
  • 2-Propanol 15 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium acetate 0.2 M -
pH 8.5
300.0 μl of 2-Propanol (50 w/v)
40.0 μl of Ammonium acetate (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
560.0 μl of water (100 v/v)
F6
  • 2-Propanol 20 w/v -
  • Sodium acetate 0.1 M pH 4.6
  • Calcium chloride 0.2 M -
pH 4.6
400.0 μl of 2-Propanol (50 w/v)
40.0 μl of Calcium chloride (5 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
460.0 μl of water (100 v/v)
F7
  • 2-Propanol 25 w/v -
  • HEPES 0.1 M pH 7.5
  • Magnesium chloride 0.1 M -
pH 7.5
500.0 μl of 2-Propanol (50 w/v)
100.0 μl of HEPES pH 7.5 (1 M)
40.0 μl of Magnesium chloride (2.5 M)
360.0 μl of water (100 v/v)
F8
  • 2-Propanol 30 w/v -
  • MES 0.1 M pH 6.5
  • tri-Sodium citrate 0.2 M -
pH 6.5
600.0 μl of 2-Propanol (50 w/v)
100.0 μl of MES pH 6.5 (1 M)
50.0 μl of tri-Sodium citrate (4 M)
250.0 μl of water (100 v/v)
F9
  • 2-Propanol 30 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium acetate 0.2 M -
pH 8.5
600.0 μl of 2-Propanol (50 w/v)
40.0 μl of Ammonium acetate (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
260.0 μl of water (100 v/v)
F10
  • 2-Methyl-2-propanol 25 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Calcium chloride 0.1 M -
pH 8.5
500.0 μl of 2-Methyl-2-propanol (50 w/v)
50.0 μl of Calcium chloride (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
350.0 μl of water (100 v/v)
F11
  • 2-Methyl-2-propanol 35 w/v -
  • tri-Sodium citrate 0.1 M pH 5.6
pH 5.6
700.0 μl of 2-Methyl-2-propanol (50 w/v)
25.0 μl of tri-Sodium citrate pH 5.6 (4 M)
275.0 μl of water (100 v/v)
F12
  • Ammonium di-hydrogen phosphate 0.2 M -
NA
66.7 μl of Ammonium di-hydrogen phosphate (3 M)
933.3 μl of water (100 v/v)
G1
  • Potassium Sodium tartrate 0.2 M -
NA
200.0 μl of Potassium Sodium tartrate (1 M)
800.0 μl of water (100 v/v)
G2
  • Magnesium acetate 0.2 M -
NA
100.0 μl of Magnesium acetate (2 M)
900.0 μl of water (100 v/v)
G3
  • Ammonium di-hydrogen phosphate 0.4 M -
NA
133.3 μl of Ammonium di-hydrogen phosphate (3 M)
866.7 μl of water (100 v/v)
G4
  • Potassium Sodium tartrate 0.4 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
400.0 μl of Potassium Sodium tartrate (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
G5
  • Ammonium di-hydrogen phosphate 0.5 M -
  • tri-Sodium citrate 0.2 M -
NA
166.7 μl of Ammonium di-hydrogen phosphate (3 M)
50.0 μl of tri-Sodium citrate (4 M)
783.3 μl of water (100 v/v)
G6
  • Sodium acetate 0.5 M -
  • Imidazole 0.1 M pH 8
pH 8
100.0 μl of Imidazole pH 8 (1 M)
200.0 μl of Sodium acetate (2.5 M)
700.0 μl of water (100 v/v)
G7
  • tri-Sodium citrate 0.7 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
175.0 μl of tri-Sodium citrate (4 M)
725.0 μl of water (100 v/v)
G8
  • Potassium Sodium tartrate 0.8 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
800.0 μl of Potassium Sodium tartrate (1 M)
100.0 μl of water (100 v/v)
G9
  • Ammonium di-hydrogen phosphate 1 M -
  • tri-Sodium citrate 0.1 M pH 5.6
pH 5.6
333.3 μl of Ammonium di-hydrogen phosphate (3 M)
25.0 μl of tri-Sodium citrate pH 5.6 (4 M)
641.7 μl of water (100 v/v)
G10
  • Ammonium di-hydrogen phosphate 1 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
333.3 μl of Ammonium di-hydrogen phosphate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
566.7 μl of water (100 v/v)
G11
  • Lithium sulfate 1 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Nickel (II) chloride 0.01 M -
pH 8.5
333.3 μl of Lithium sulfate (3 M)
10.0 μl of Nickel (II) chloride (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
556.7 μl of water (100 v/v)
G12
  • Sodium acetate 1 M -
  • Imidazole 0.1 M pH 8
pH 8
100.0 μl of Imidazole pH 8 (1 M)
400.0 μl of Sodium acetate (2.5 M)
500.0 μl of water (100 v/v)
H1
  • Sodium acetate 1.4 M -
  • MES 0.1 M pH 6.5
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
560.0 μl of Sodium acetate (2.5 M)
340.0 μl of water (100 v/v)
H2
  • Lithium sulfate 1.5 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
500.0 μl of Lithium sulfate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
H3
  • tri-Sodium citrate 1.5 M -
NA
750.0 μl of tri-Sodium citrate pH 6.5 (2 M)
250.0 μl of water (100 v/v)
H4
  • Magnesium sulfate 1.6 M -
  • MES 0.1 M pH 6.5
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
800.0 μl of Magnesium sulfate (2 M)
100.0 μl of water (100 v/v)
H5
  • Potassium Sodium tartrate 1.6 M -
  • MES 0.1 M pH 6.5
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
800.0 μl of Potassium Sodium tartrate (2 M)
100.0 μl of water (100 v/v)
H6
  • Ammonium formate 2 M -
  • MES 0.1 M pH 6.5
pH 6.5
400.0 μl of Ammonium formate (5 M)
100.0 μl of MES pH 6.5 (1 M)
500.0 μl of water (100 v/v)
H7
  • Ammonium di-hydrogen phosphate 2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
666.7 μl of Ammonium di-hydrogen phosphate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
233.3 μl of water (100 v/v)
H8
  • Sodium chloride 2 M -
  • MES 0.1 M pH 6.5
  • Sodium acetate 0.2 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
80.0 μl of Sodium acetate (2.5 M)
400.0 μl of Sodium chloride (5 M)
420.0 μl of water (100 v/v)
H9
  • Sodium formate 2 M -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
100.0 μl of Sodium acetate pH 4.6 (1 M)
285.7 μl of Sodium formate (7 M)
614.3 μl of water (100 v/v)
H10
  • Ammonium di-hydrogen phosphate 1 M -
  • Glycerol 30 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
333.3 μl of Ammonium di-hydrogen phosphate (3 M)
375.0 μl of Glycerol (80 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
191.7 μl of water (100 v/v)
H11
  • Sodium chloride 4 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
800.0 μl of Sodium chloride (5 M)
100.0 μl of water (100 v/v)
H12
  • Sodium formate 3 M -
NA
428.6 μl of Sodium formate (7 M)
571.4 μl of water (100 v/v)