C6 - Screen Contents

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Supplemental Information

Screen Description

Cryo 1

Cryos / Cryo 1

Created 2009-11-16.

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Cryo 1

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Cryo 1

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Screen Structure

Cryo 1

Well Count	24
Number of distinct ingredients	25
Internal diversity (score mean dist)	0.762

Screen Composition

More information about screen composition chart.

ingredient type	Cryo 1
Buffer	22
Cryoprotectant	24
Organic (non-volatile)	26
Organic (volatile)	3
Polymer	19
Salt	20

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Cryo 1 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Glycerol 2.8 v/v - Polyethylene glycol 8,000 32 w/v - Cesium chloride 0.2 M - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	200.0 μl of Cesium chloride (1 M) 28.0 μl of Glycerol (100 v/v) 640.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 32.0 μl of water (100 v/v)
A2	Glycerol 5 v/v - Sodium formate 3.2 M - tri-Sodium citrate 0.1 M pH 5.6 🔍	pH 5.6	50.0 μl of Glycerol (100 v/v) 457.1 μl of Sodium formate (7 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 392.9 μl of water (100 v/v)
A3	Glycerol 5 v/v - Polyethylene glycol 4,000 20 w/v - 🔍	NA	50.0 μl of Glycerol (100 v/v) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 550.0 μl of water (100 v/v)
A4	Glycerol 10 v/v - Polyethylene glycol monomethyl ether 5,000 15 w/v - Xylitol 10 w/v - 🔍	NA	100.0 μl of Glycerol (100 v/v) 300.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 333.3 μl of Xylitol (30 w/v) 266.7 μl of water (100 v/v)
A5	Glycerol 10 v/v - Polyethylene glycol 8,000 28 w/v - Ammonium acetate 0.1 M - MES 0.05 M pH 6.5 🔍	pH 6.5	20.0 μl of Ammonium acetate (5 M) 100.0 μl of Glycerol (100 v/v) 16.7 μl of MES pH 6.5 (3 M) 560.0 μl of Polyethylene glycol 8,000 (50 w/v) 303.3 μl of water (100 v/v)
A6	Glycerol 10 v/v - Polyethylene glycol 4,000 25 w/v - 2-Propanol 4.5 v/v - MES 0.018 M pH 6.5 🔍	pH 6.5	45.0 μl of 2-Propanol (100 v/v) 100.0 μl of Glycerol (100 v/v) 6.0 μl of MES pH 6.5 (3 M) 500.0 μl of Polyethylene glycol 4,000 (50 w/v) 349.0 μl of water (100 v/v)
B1	Glycerol 10 v/v - Polyethylene glycol 8,000 10 w/v - Sodium chloride 0.1 M - tris chloride BCC 0.05 M pH 8.5 Polyethylene glycol 400 20 v/v - 🔍	pH 8.5	100.0 μl of Glycerol (100 v/v) 200.0 μl of Polyethylene glycol 400 (100 v/v) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 20.0 μl of Sodium chloride (5 M) 50.0 μl of tris chloride BCC pH 8.5 (1 M) 430.0 μl of water (100 v/v)
B2	Glycerol 15 v/v - Polyethylene glycol 6,000 15 w/v - Lithium chloride 1 M - MES 0.1 M pH 6.5 🔍	pH 6.5	150.0 μl of Glycerol (100 v/v) 200.0 μl of Lithium chloride (5 M) 33.3 μl of MES pH 6.5 (3 M) 300.0 μl of Polyethylene glycol 6,000 (50 w/v) 316.7 μl of water (100 v/v)
B3	Glycerol 15 v/v - Polyethylene glycol monomethyl ether 5,000 15 w/v - 🔍	NA	150.0 μl of Glycerol (100 v/v) 300.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 550.0 μl of water (100 v/v)
B4	Glycerol 20 v/v - Polyethylene glycol 6,000 20 w/v - MES 0.1 M pH 6.5 🔍	pH 6.5	200.0 μl of Glycerol (100 v/v) 33.3 μl of MES pH 6.5 (3 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 366.7 μl of water (100 v/v)
B5	Glycerol 15 v/v - Sodium acetate 0.2 M - MES 0.1 M pH 6.5 Polyethylene glycol 8,000 30 w/v - 🔍	pH 6.5	150.0 μl of Glycerol (100 v/v) 33.3 μl of MES pH 6.5 (3 M) 600.0 μl of Polyethylene glycol 8,000 (50 w/v) 80.0 μl of Sodium acetate (2.5 M) 136.7 μl of water (100 v/v)
B6	Glycerol 15 v/v - Sodium chloride 2.5 M - Lithium sulfate 0.2 M - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	150.0 μl of Glycerol (100 v/v) 66.7 μl of Lithium sulfate (3 M) 500.0 μl of Sodium chloride (5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 183.3 μl of water (100 v/v)
C1	Glycerol 15 v/v - Polyethylene glycol 20,000 15 w/v - 🔍	NA	150.0 μl of Glycerol (100 v/v) 300.0 μl of Polyethylene glycol 20,000 (50 w/v) 550.0 μl of water (100 v/v)
C2	Glycerol 15 v/v - Polyethylene glycol 4,000 35 w/v - tri-Sodium citrate 0.2 M - TRIS-Acetate 0.1 M pH 8 🔍	pH 8	150.0 μl of Glycerol (100 v/v) 700.0 μl of Polyethylene glycol 4,000 (50 w/v) 50.0 μl of TRIS-Acetate pH 8 (2 M) 40.0 μl of tri-Sodium citrate (5 M) 60.0 μl of water (100 v/v)
C3	Glycerol 15 v/v - Polyethylene glycol 8,000 15 w/v - Lithium sulfate 0.5 M - 🔍	NA	150.0 μl of Glycerol (100 v/v) 166.7 μl of Lithium sulfate (3 M) 300.0 μl of Polyethylene glycol 8,000 (50 w/v) 383.3 μl of water (100 v/v)
C4	Glycerol 20 v/v - Ethylene glycol 16 v/v - Polyethylene glycol 6,000 20 w/v - Sodium acetate 0.1 M - tris chloride BCC 0.03 M pH 8.5 🔍	pH 8.5	160.0 μl of Ethylene glycol (100 v/v) 200.0 μl of Glycerol (100 v/v) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 40.0 μl of Sodium acetate (2.5 M) 30.0 μl of tris chloride BCC pH 8.5 (1 M) 170.0 μl of water (100 v/v)
C5	Glycerol 20 v/v - Ammonium sulfate 2 M - 🔍	NA	666.7 μl of Ammonium sulfate (3 M) 200.0 μl of Glycerol (100 v/v) 133.3 μl of water (100 v/v)
C6	Glycerol 20 v/v - Polyethylene glycol 4,000 20 w/v - 2-Propanol 10 v/v - Sodium chloride 0.1 M - Sodium acetate 0.05 M pH 4.6 🔍	pH 4.6	100.0 μl of 2-Propanol (100 v/v) 200.0 μl of Glycerol (100 v/v) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 50.0 μl of Sodium acetate pH 4.6 (1 M) 100.0 μl of Sodium chloride (1 M) 150.0 μl of water (100 v/v)
D1	Glycerol 20 v/v - Ammonium sulfate 2.5 M - MES 0.1 M pH 6.5 🔍	pH 6.5	625.0 μl of Ammonium sulfate (4 M) 200.0 μl of Glycerol (100 v/v) 33.3 μl of MES pH 6.5 (3 M) 141.7 μl of water (100 v/v)
D2	Glycerol 20 v/v - Polyethylene glycol 8,000 15 w/v - Zinc acetate 0.16 M - MES 0.1 M pH 6.5 🔍	pH 6.5	200.0 μl of Glycerol (100 v/v) 33.3 μl of MES pH 6.5 (3 M) 300.0 μl of Polyethylene glycol 8,000 (50 w/v) 80.0 μl of Zinc acetate (2 M) 386.7 μl of water (100 v/v)
D3	Glycerol 20 v/v - Polyethylene glycol 8,000 15 w/v - Sodium chloride 0.92 M - tris chloride BCC 0.08 M pH 8.5 🔍	pH 8.5	200.0 μl of Glycerol (100 v/v) 300.0 μl of Polyethylene glycol 8,000 (50 w/v) 184.0 μl of Sodium chloride (5 M) 80.0 μl of tris chloride BCC pH 8.5 (1 M) 236.0 μl of water (100 v/v)
D4	Glycerol 20 v/v - 2-Propanol 6 v/v - Ammonium sulfate 1.8 M - 🔍	NA	60.0 μl of 2-Propanol (100 v/v) 600.0 μl of Ammonium sulfate (3 M) 200.0 μl of Glycerol (100 v/v) 140.0 μl of water (100 v/v)
D5	Glycerol 20 v/v - Ammonium sulfate 1.8 M - MES 0.1 M pH 6.5 🔍	pH 6.5	600.0 μl of Ammonium sulfate (3 M) 200.0 μl of Glycerol (100 v/v) 33.3 μl of MES pH 6.5 (3 M) 166.7 μl of water (100 v/v)
D6	Glycerol 20 v/v - Polyethylene glycol 6,000 20 w/v - MES 0.1 M pH 6.5 🔍	pH 6.5	200.0 μl of Glycerol (100 v/v) 33.3 μl of MES pH 6.5 (3 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 366.7 μl of water (100 v/v)