C6 - Cryo 2 - Screen Contents

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Supplemental Information

Screen Description

Cryos / Cryo 2

Created 2009-11-16.

Rigaku Recipe XML

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Screen Structure

Well Count	24
Number of distinct ingredients	19
Internal diversity (score mean dist)	0.689

Screen Composition

More information about screen composition chart.

ingredient type	Cryo 2
Buffer	23
Cryoprotectant	24
Organic (non-volatile)	24
Organic (volatile)	2
Polymer	8
Salt	22

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Cryo 2 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Glycerol 20 v/v - Polyethylene glycol 8,000 16 w/v - MES 0.08 M pH 6.5 Magnesium acetate 0.16 M - 🔍	pH 6.5	200.0 μl of Glycerol (100 v/v) 80.0 μl of Magnesium acetate (2 M) 80.0 μl of MES pH 6.5 (1 M) 320.0 μl of Polyethylene glycol 8,000 (50 w/v) 320.0 μl of water (100 v/v)
A2	Glycerol 20 v/v - Polyethylene glycol 8,000 7 w/v - MES 0.1 M pH 6.5 🔍	pH 6.5	200.0 μl of Glycerol (100 v/v) 100.0 μl of MES pH 6.5 (1 M) 140.0 μl of Polyethylene glycol 8,000 (50 w/v) 560.0 μl of water (100 v/v)
A3	Glycerol 24 v/v - Ammonium sulfate 2.3 M - 🔍	NA	575.0 μl of Ammonium sulfate (4 M) 240.0 μl of Glycerol (100 v/v) 185.0 μl of water (100 v/v)
A4	Glycerol 25 v/v - Sodium bromide 0.75 M - 🔍	NA	250.0 μl of Glycerol (100 v/v) 150.0 μl of Sodium bromide (5 M) 600.0 μl of water (100 v/v)
A5	Glycerol 25 v/v - tri-Sodium citrate 0.1 M pH 5.6 Ammonium di-hydrogen phosphate 1.2 M - 🔍	pH 5.6	400.0 μl of Ammonium di-hydrogen phosphate (3 M) 250.0 μl of Glycerol (100 v/v) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 250.0 μl of water (100 v/v)
A6	Glycerol 25 v/v - Ammonium sulfate 1.5 M - 🔍	NA	375.0 μl of Ammonium sulfate (4 M) 250.0 μl of Glycerol (100 v/v) 375.0 μl of water (100 v/v)
B1	Glycerol 25 v/v - MES 0.1 M pH 6.5 Ammonium sulfate 2.2 M - 🔍	pH 6.5	550.0 μl of Ammonium sulfate (4 M) 250.0 μl of Glycerol (100 v/v) 100.0 μl of MES pH 6.5 (1 M) 100.0 μl of water (100 v/v)
B2	Glycerol 25 v/v - Sodium Potassium phosphate 0.1 M pH 6.5 Ammonium sulfate 2.5 M - 🔍	pH 6.5	625.0 μl of Ammonium sulfate (4 M) 250.0 μl of Glycerol (100 v/v) 25.0 μl of Sodium Potassium phosphate pH 6.5 (4 M) 100.0 μl of water (100 v/v)
B3	Glycerol 30 v/v - Ammonium di-hydrogen phosphate 1.26 M - 🔍	NA	420.0 μl of Ammonium di-hydrogen phosphate (3 M) 300.0 μl of Glycerol (100 v/v) 280.0 μl of water (100 v/v)
B4	Glycerol 30 v/v - Sodium acetate 0.1 M pH 4.6 Ammonium sulfate 2 M - 🔍	pH 4.6	500.0 μl of Ammonium sulfate (4 M) 300.0 μl of Glycerol (100 v/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 100.0 μl of water (100 v/v)
B5	Glycerol 30 v/v - MES 0.05 M pH 6.5 Ammonium sulfate 2.3 M - 🔍	pH 6.5	575.0 μl of Ammonium sulfate (4 M) 300.0 μl of Glycerol (100 v/v) 50.0 μl of MES pH 6.5 (1 M) 75.0 μl of water (100 v/v)
B6	Glycerol 30 v/v - Ammonium sulfate 2 M - 🔍	NA	500.0 μl of Ammonium sulfate (4 M) 300.0 μl of Glycerol (100 v/v) 200.0 μl of water (100 v/v)
C1	Glycerol 30 v/v - Polyethylene glycol 4,000 15 w/v - 2-Propanol 5 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	50.0 μl of 2-Propanol (100 v/v) 300.0 μl of Glycerol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 300.0 μl of Polyethylene glycol 4,000 (50 w/v) 250.0 μl of water (100 v/v)
C2	Glycerol 30 v/v - Polyethylene glycol 4,000 20 w/v - 2-Propanol 20 v/v - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	200.0 μl of 2-Propanol (100 v/v) 300.0 μl of Glycerol (100 v/v) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M)
C3	Glycerol 30 v/v - MES 0.05 M pH 6.5 Ammonium sulfate 1.2 M - 🔍	pH 6.5	400.0 μl of Ammonium sulfate (3 M) 300.0 μl of Glycerol (100 v/v) 50.0 μl of MES pH 6.5 (1 M) 250.0 μl of water (100 v/v)
C4	Glycerol 30 v/v - Polyethylene glycol 6,000 15 w/v - tris chloride BCC 0.5 M pH 8.5 🔍	pH 8.5	300.0 μl of Glycerol (100 v/v) 300.0 μl of Polyethylene glycol 6,000 (50 w/v) 166.7 μl of tris chloride BCC pH 8.5 (3 M) 233.3 μl of water (100 v/v)
C5	Glycerol 30 v/v - Ammonium sulfate 2 M - 🔍	NA	500.0 μl of Ammonium sulfate (4 M) 300.0 μl of Glycerol (100 v/v) 200.0 μl of water (100 v/v)
C6	Glycerol 30 v/v - Polyethylene glycol 1,500 30 w/v - MES 0.05 M pH 6.5 🔍	pH 6.5	300.0 μl of Glycerol (100 v/v) 50.0 μl of MES pH 6.5 (1 M) 600.0 μl of Polyethylene glycol 1,500 (50 w/v) 50.0 μl of water (100 v/v)
D1	Glycerol 30 v/v - Sodium Potassium phosphate 0.1 M pH 6.5 Ammonium sulfate 1 M - 🔍	pH 6.5	250.0 μl of Ammonium sulfate (4 M) 300.0 μl of Glycerol (100 v/v) 25.0 μl of Sodium Potassium phosphate pH 6.5 (4 M) 425.0 μl of water (100 v/v)
D2	Glycerol 30 v/v - Polyethylene glycol 4,000 20 w/v - Sodium acetate 0.1 M pH 4.6 🔍	pH 4.6	300.0 μl of Glycerol (100 v/v) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 200.0 μl of water (100 v/v)
D3	Glycerol 30 v/v - MES 0.1 M pH 6.5 Sodium acetate 1.4 M - 🔍	pH 6.5	300.0 μl of Glycerol (100 v/v) 100.0 μl of MES pH 6.5 (1 M) 560.0 μl of Sodium acetate (2.5 M) 40.0 μl of water (100 v/v)
D4	Glycerol 30 v/v - HEPES 0.15 M pH 7.5 Sodium di-hydrogen phosphate 1.6 M - di-Potassium hydrogen phosphate 0.45 M - 🔍	pH 7.5	90.0 μl of di-Potassium hydrogen phosphate (5 M) 300.0 μl of Glycerol (100 v/v) 150.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Sodium di-hydrogen phosphate (4 M) 60.0 μl of water (100 v/v)
D5	Glycerol 35 v/v - HEPES 0.02 M pH 7.5 🔍	pH 7.5	350.0 μl of Glycerol (100 v/v) 20.0 μl of HEPES pH 7.5 (1 M) 630.0 μl of water (100 v/v)
D6	Glycerol 35 v/v - Polyethylene glycol 6,000 7 w/v - HEPES 0.05 M pH 6.5 🔍	pH 6.5	350.0 μl of Glycerol (100 v/v) 50.0 μl of HEPES pH 6.5 (1 M) 140.0 μl of Polyethylene glycol 6,000 (50 w/v) 460.0 μl of water (100 v/v)