C6 - Screen Contents

Welcome to the new C6 website. This website aims preserve as many features as possible of the original C6 website.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.

Jump to well contents.

Supplemental Information

Screen Description

Cryo 2

Cryos / Cryo 2

Created 2009-11-16.

Export

Cryo 2

Barcodes

Cryo 2

No barcodes found.

Screen Structure

Cryo 2

Well Count	24
Number of distinct ingredients	19
Internal diversity (score mean dist)	0.689

Screen Composition

More information about screen composition chart.

ingredient type	Cryo 2
Buffer	23
Cryoprotectant	24
Organic (non-volatile)	24
Organic (volatile)	2
Polymer	8
Salt	22

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Cryo 2 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Glycerol 20 v/v - Polyethylene glycol 8,000 16 w/v - MES 0.08 M pH 6.5 Magnesium acetate 0.16 M - 🔍	pH 6.5	200.0 μl of Glycerol (100 v/v) 80.0 μl of MES pH 6.5 (1 M) 80.0 μl of Magnesium acetate (2 M) 320.0 μl of Polyethylene glycol 8,000 (50 w/v) 320.0 μl of water (100 v/v)
A2	Glycerol 20 v/v - Polyethylene glycol 8,000 7 w/v - MES 0.1 M pH 6.5 🔍	pH 6.5	200.0 μl of Glycerol (100 v/v) 100.0 μl of MES pH 6.5 (1 M) 140.0 μl of Polyethylene glycol 8,000 (50 w/v) 560.0 μl of water (100 v/v)
A3	Glycerol 24 v/v - Ammonium sulfate 2.3 M - 🔍	NA	575.0 μl of Ammonium sulfate (4 M) 240.0 μl of Glycerol (100 v/v) 185.0 μl of water (100 v/v)
A4	Glycerol 25 v/v - Sodium bromide 0.75 M - 🔍	NA	250.0 μl of Glycerol (100 v/v) 150.0 μl of Sodium bromide (5 M) 600.0 μl of water (100 v/v)
A5	Glycerol 25 v/v - tri-Sodium citrate 0.1 M pH 5.6 Ammonium di-hydrogen phosphate 1.2 M - 🔍	pH 5.6	400.0 μl of Ammonium di-hydrogen phosphate (3 M) 250.0 μl of Glycerol (100 v/v) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 250.0 μl of water (100 v/v)
A6	Glycerol 25 v/v - Ammonium sulfate 1.5 M - 🔍	NA	375.0 μl of Ammonium sulfate (4 M) 250.0 μl of Glycerol (100 v/v) 375.0 μl of water (100 v/v)
B1	Glycerol 25 v/v - MES 0.1 M pH 6.5 Ammonium sulfate 2.2 M - 🔍	pH 6.5	550.0 μl of Ammonium sulfate (4 M) 250.0 μl of Glycerol (100 v/v) 100.0 μl of MES pH 6.5 (1 M) 100.0 μl of water (100 v/v)
B2	Glycerol 25 v/v - Sodium Potassium phosphate 0.1 M pH 6.5 Ammonium sulfate 2.5 M - 🔍	pH 6.5	625.0 μl of Ammonium sulfate (4 M) 250.0 μl of Glycerol (100 v/v) 25.0 μl of Sodium Potassium phosphate pH 6.5 (4 M) 100.0 μl of water (100 v/v)
B3	Glycerol 30 v/v - Ammonium di-hydrogen phosphate 1.26 M - 🔍	NA	420.0 μl of Ammonium di-hydrogen phosphate (3 M) 300.0 μl of Glycerol (100 v/v) 280.0 μl of water (100 v/v)
B4	Glycerol 30 v/v - Sodium acetate 0.1 M pH 4.6 Ammonium sulfate 2 M - 🔍	pH 4.6	500.0 μl of Ammonium sulfate (4 M) 300.0 μl of Glycerol (100 v/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 100.0 μl of water (100 v/v)
B5	Glycerol 30 v/v - MES 0.05 M pH 6.5 Ammonium sulfate 2.3 M - 🔍	pH 6.5	575.0 μl of Ammonium sulfate (4 M) 300.0 μl of Glycerol (100 v/v) 50.0 μl of MES pH 6.5 (1 M) 75.0 μl of water (100 v/v)
B6	Glycerol 30 v/v - Ammonium sulfate 2 M - 🔍	NA	500.0 μl of Ammonium sulfate (4 M) 300.0 μl of Glycerol (100 v/v) 200.0 μl of water (100 v/v)
C1	Glycerol 30 v/v - Polyethylene glycol 4,000 15 w/v - 2-Propanol 5 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	50.0 μl of 2-Propanol (100 v/v) 300.0 μl of Glycerol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 300.0 μl of Polyethylene glycol 4,000 (50 w/v) 250.0 μl of water (100 v/v)
C2	Glycerol 30 v/v - Polyethylene glycol 4,000 20 w/v - 2-Propanol 20 v/v - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	200.0 μl of 2-Propanol (100 v/v) 300.0 μl of Glycerol (100 v/v) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M)
C3	Glycerol 30 v/v - MES 0.05 M pH 6.5 Ammonium sulfate 1.2 M - 🔍	pH 6.5	400.0 μl of Ammonium sulfate (3 M) 300.0 μl of Glycerol (100 v/v) 50.0 μl of MES pH 6.5 (1 M) 250.0 μl of water (100 v/v)
C4	Glycerol 30 v/v - Polyethylene glycol 6,000 15 w/v - tris chloride BCC 0.5 M pH 8.5 🔍	pH 8.5	300.0 μl of Glycerol (100 v/v) 300.0 μl of Polyethylene glycol 6,000 (50 w/v) 166.7 μl of tris chloride BCC pH 8.5 (3 M) 233.3 μl of water (100 v/v)
C5	Glycerol 30 v/v - Ammonium sulfate 2 M - 🔍	NA	500.0 μl of Ammonium sulfate (4 M) 300.0 μl of Glycerol (100 v/v) 200.0 μl of water (100 v/v)
C6	Glycerol 30 v/v - Polyethylene glycol 1,500 30 w/v - MES 0.05 M pH 6.5 🔍	pH 6.5	300.0 μl of Glycerol (100 v/v) 50.0 μl of MES pH 6.5 (1 M) 600.0 μl of Polyethylene glycol 1,500 (50 w/v) 50.0 μl of water (100 v/v)
D1	Glycerol 30 v/v - Sodium Potassium phosphate 0.1 M pH 6.5 Ammonium sulfate 1 M - 🔍	pH 6.5	250.0 μl of Ammonium sulfate (4 M) 300.0 μl of Glycerol (100 v/v) 25.0 μl of Sodium Potassium phosphate pH 6.5 (4 M) 425.0 μl of water (100 v/v)
D2	Glycerol 30 v/v - Polyethylene glycol 4,000 20 w/v - Sodium acetate 0.1 M pH 4.6 🔍	pH 4.6	300.0 μl of Glycerol (100 v/v) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 200.0 μl of water (100 v/v)
D3	Glycerol 30 v/v - MES 0.1 M pH 6.5 Sodium acetate 1.4 M - 🔍	pH 6.5	300.0 μl of Glycerol (100 v/v) 100.0 μl of MES pH 6.5 (1 M) 560.0 μl of Sodium acetate (2.5 M) 40.0 μl of water (100 v/v)
D4	Glycerol 30 v/v - HEPES 0.15 M pH 7.5 Sodium di-hydrogen phosphate 1.6 M - di-Potassium hydrogen phosphate 0.45 M - 🔍	pH 7.5	300.0 μl of Glycerol (100 v/v) 150.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Sodium di-hydrogen phosphate (4 M) 90.0 μl of di-Potassium hydrogen phosphate (5 M) 60.0 μl of water (100 v/v)
D5	Glycerol 35 v/v - HEPES 0.02 M pH 7.5 🔍	pH 7.5	350.0 μl of Glycerol (100 v/v) 20.0 μl of HEPES pH 7.5 (1 M) 630.0 μl of water (100 v/v)
D6	Glycerol 35 v/v - Polyethylene glycol 6,000 7 w/v - HEPES 0.05 M pH 6.5 🔍	pH 6.5	350.0 μl of Glycerol (100 v/v) 50.0 μl of HEPES pH 6.5 (1 M) 140.0 μl of Polyethylene glycol 6,000 (50 w/v) 460.0 μl of water (100 v/v)