C6 - Screen Contents

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Supplemental Information

Screen Description

Cryo 3

Cryos / Cryo 3

Created 2009-11-16.

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Cryo 3

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Cryo 3

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Screen Structure

Cryo 3

Well Count	24
Number of distinct ingredients	34
Internal diversity (score mean dist)	0.947

Screen Composition

More information about screen composition chart.

ingredient type	Cryo 3
Buffer	22
Organic (non-volatile)	16
Organic (volatile)	2
Polymer	32
Salt	18

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Cryo 3 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Polyethylene glycol 200 10 v/v - Polyethylene glycol 3,000 24 w/v - tri-Sodium citrate 0.1 M pH 5.6 🔍	pH 5.6	100.0 μl of Polyethylene glycol 200 (100 v/v) 480.0 μl of Polyethylene glycol 3,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 320.0 μl of water (100 v/v)
A2	Polyethylene glycol 200 20 v/v - Polyethylene glycol 4,000 20 w/v - 2-Propanol 10 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100.0 μl of 2-Propanol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 200 (100 v/v) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 200.0 μl of water (100 v/v)
A3	Polyethylene glycol 200 30 v/v - Polyethylene glycol 3,000 5 w/v - MES 0.1 M pH 6.5 🔍	pH 6.5	33.3 μl of MES pH 6.5 (3 M) 300.0 μl of Polyethylene glycol 200 (100 v/v) 100.0 μl of Polyethylene glycol 3,000 (50 w/v) 566.7 μl of water (100 v/v)
A4	Polyethylene glycol 300 40 v/v - Polyethylene glycol 1,000 5 w/v - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	100.0 μl of Polyethylene glycol 1,000 (50 w/v) 400.0 μl of Polyethylene glycol 300 (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 400.0 μl of water (100 v/v)
A5	Polyethylene glycol 400 20 v/v - Polyethylene glycol 4,000 30 w/v - tri-Sodium citrate 0.1 M pH 5.6 Ammonium acetate 0.05 M - 🔍	pH 5.6	10.0 μl of Ammonium acetate (5 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 200.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 90.0 μl of water (100 v/v)
A6	Polyethylene glycol 400 30 v/v - Polyethylene glycol 20,000 11 w/v - di-Potassium hydrogen phosphate 0.1 M pH 8 🔍	pH 8	220.0 μl of Polyethylene glycol 20,000 (50 w/v) 300.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of di-Potassium hydrogen phosphate pH 8 (1 M) 380.0 μl of water (100 v/v)
B1	Polyethylene glycol 400 10 v/v - Polyethylene glycol monomethyl ether 5,000 20 w/v - 🔍	NA	100.0 μl of Polyethylene glycol 400 (100 v/v) 400.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 500.0 μl of water (100 v/v)
B2	Polyethylene glycol 400 34 v/v - HEPES 0.1 M pH 7.5 Sodium chloride 0.1 M - Cadmium chloride 0.2 M - 🔍	pH 7.5	200.0 μl of Cadmium chloride (1 M) 100.0 μl of HEPES pH 7.5 (1 M) 340.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of Sodium chloride (1 M) 260.0 μl of water (100 v/v)
B3	Polyethylene glycol 400 40 v/v - Polyethylene glycol 3,000 5 w/v - HEPES 0.1 M pH 6.5 🔍	pH 6.5	100.0 μl of HEPES pH 6.5 (1 M) 100.0 μl of Polyethylene glycol 3,000 (50 w/v) 400.0 μl of Polyethylene glycol 400 (100 v/v) 400.0 μl of water (100 v/v)
B4	Polyethylene glycol 600 35 w/v - PIPES 0.1 M pH 7 Sodium chloride 0.1 M - 🔍	pH 7	100.0 μl of PIPES pH 7 (1 M) 700.0 μl of Polyethylene glycol 600 (50 w/v) 100.0 μl of Sodium chloride (1 M) 100.0 μl of water (100 v/v)
B5	Polyethylene glycol 1,000 30 w/v - Sodium phosphate citrate 0.1 M pH 4.4 Lithium sulfate 0.11 M - 🔍	pH 4.4	36.7 μl of Lithium sulfate (3 M) 600.0 μl of Polyethylene glycol 1,000 (50 w/v) 100.0 μl of Sodium phosphate citrate pH 4.4 (1 M) 263.3 μl of water (100 v/v)
B6	Polyethylene glycol 1,500 40 w/v - ADA 0.1 M pH 6 🔍	pH 6	100.0 μl of ADA pH 6 (1 M) 800.0 μl of Polyethylene glycol 1,500 (50 w/v) 100.0 μl of water (100 v/v)
C1	Polyethylene glycol 1,500 17 w/v - 2-methyl-2,4-pentanediol BCC 10 v/v - PIPES 0.05 M pH 7 🔍	pH 7	100.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 50.0 μl of PIPES pH 7 (1 M) 340.0 μl of Polyethylene glycol 1,500 (50 w/v) 510.0 μl of water (100 v/v)
C2	Polyethylene glycol 3,350 35 w/v - Potassium thiocyanate 0.3 M - 🔍	NA	700.0 μl of Polyethylene glycol 3,350 (50 w/v) 37.5 μl of Potassium thiocyanate (8 M) 262.5 μl of water (100 v/v)
C3	Polyethylene glycol 4,000 25 w/v - 2-methyl-2,4-pentanediol BCC 18 v/v - Sodium acetate 0.1 M pH 4.6 Ammonium sulfate 0.2 M - 🔍	pH 4.6	180.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 66.7 μl of Ammonium sulfate (3 M) 500.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 153.3 μl of water (100 v/v)
C4	Polyethylene glycol 4,000 25 w/v - Ethylene glycol 30 v/v - 🔍	NA	300.0 μl of Ethylene glycol (100 v/v) 500.0 μl of Polyethylene glycol 4,000 (50 w/v) 200.0 μl of water (100 v/v)
C5	Polyethylene glycol 4,000 20 w/v - 2-methyl-2,4-pentanediol BCC 20 v/v - 2-Propanol 10 v/v - tri-Sodium citrate 0.1 M pH 5.6 🔍	pH 5.6	100.0 μl of 2-Propanol (100 v/v) 200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 200.0 μl of water (100 v/v)
C6	Polyethylene glycol 4,000 10 w/v - Ethylene glycol 20 v/v - ADA 0.05 M pH 6 Sodium chloride 0.105 M - 🔍	pH 6	50.0 μl of ADA pH 6 (1 M) 200.0 μl of Ethylene glycol (100 v/v) 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 105.0 μl of Sodium chloride (1 M) 445.0 μl of water (100 v/v)
D1	Polyethylene glycol 4,000 2 w/v - 2-methyl-2,4-pentanediol BCC 25 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	250.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 40.0 μl of Polyethylene glycol 4,000 (50 w/v) 610.0 μl of water (100 v/v)
D2	Polyethylene glycol 8,000 20 w/v - 2-methyl-2,4-pentanediol BCC 20 v/v - HEPES 0.1 M pH 7.5 Rubidium bromide 0.2 M - 🔍	pH 7.5	200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 200.0 μl of Rubidium bromide (1 M) 100.0 μl of water (100 v/v)
D3	Polyethylene glycol 8,000 28 w/v - Glycine 0.1 M pH 9.5 Calcium chloride 0.01 M - 🔍	pH 9.5	10.0 μl of Calcium chloride (1 M) 50.0 μl of Glycine pH 9.5 (2 M) 560.0 μl of Polyethylene glycol 8,000 (50 w/v) 380.0 μl of water (100 v/v)
D4	Polyethylene glycol 8,000 10 w/v - 2-methyl-2,4-pentanediol BCC 15 v/v - MES 0.1 M pH 6.5 Ammonium sulfate 0.55 M - 🔍	pH 6.5	150.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 183.3 μl of Ammonium sulfate (3 M) 33.3 μl of MES pH 6.5 (3 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 433.3 μl of water (100 v/v)
D5	Polyethylene glycol 8,000 25 w/v - Ethylene glycol 30 v/v - ADA 0.15 M pH 6 Ammonium sulfate 0.08 M - 🔍	pH 6	150.0 μl of ADA pH 6 (1 M) 26.7 μl of Ammonium sulfate (3 M) 300.0 μl of Ethylene glycol (100 v/v) 500.0 μl of Polyethylene glycol 8,000 (50 w/v) 23.3 μl of water (100 v/v)
D6	Polyethylene glycol 20,000 10 w/v - Ethylene glycol 5.6 v/v - 2,3-Butanediol 30 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	300.0 μl of 2,3-Butanediol (100 v/v) 56.0 μl of Ethylene glycol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 20,000 (50 w/v) 344.0 μl of water (100 v/v)