C6 - Screen Contents

Welcome to the new C6 website. This website aims preserve as many features as possible of the original C6 website.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.

Jump to well contents.

Supplemental Information

Screen Description

Cryo 3

Cryos / Cryo 3

Created 2009-11-16.

Export

Cryo 3

Barcodes

Cryo 3

Screen Structure

Cryo 3

Well Count	24
Number of distinct ingredients	34
Internal diversity (score mean dist)	0.947

Screen Composition

More information about screen composition chart.

ingredient type	Cryo 3
Buffer	22
Organic (non-volatile)	16
Organic (volatile)	2
Polymer	32
Salt	18

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Cryo 3 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Polyethylene glycol 200 10 v/v - Polyethylene glycol 3,000 24 w/v - tri-Sodium citrate 0.1 M pH 5.6 🔍	pH 5.6	480 μl of Polyethylene glycol 3,000 (50 w/v) 100 μl of Polyethylene glycol 200 (100 v/v) 100 μl of tri-Sodium citrate pH 5.6 (1 M) 320 μl of water (100 v/v)
A2	Polyethylene glycol 200 20 v/v - Polyethylene glycol 4,000 20 w/v - 2-Propanol 10 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100 μl of 2-Propanol (100 v/v) 100 μl of HEPES pH 7.5 (1 M) 400 μl of Polyethylene glycol 4,000 (50 w/v) 200 μl of Polyethylene glycol 200 (100 v/v) 200 μl of water (100 v/v)
A3	Polyethylene glycol 200 30 v/v - Polyethylene glycol 3,000 5 w/v - MES 0.1 M pH 6.5 🔍	pH 6.5	100 μl of Polyethylene glycol 3,000 (50 w/v) 33 μl of MES pH 6.5 (3 M) 300 μl of Polyethylene glycol 200 (100 v/v) 566 μl of water (100 v/v)
A4	Polyethylene glycol 300 40 v/v - Polyethylene glycol 1,000 5 w/v - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	400 μl of Polyethylene glycol 300 (100 v/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 100 μl of Polyethylene glycol 1,000 (50 w/v) 400 μl of water (100 v/v)
A5	Polyethylene glycol 400 20 v/v - Polyethylene glycol 4,000 30 w/v - tri-Sodium citrate 0.1 M pH 5.6 Ammonium acetate 0.05 M - 🔍	pH 5.6	10 μl of Ammonium acetate (5 M) 600 μl of Polyethylene glycol 4,000 (50 w/v) 200 μl of Polyethylene glycol 400 (100 v/v) 100 μl of tri-Sodium citrate pH 5.6 (1 M) 90 μl of water (100 v/v)
A6	Polyethylene glycol 400 30 v/v - Polyethylene glycol 20,000 11 w/v - di-Potassium hydrogen phosphate 0.1 M pH 8 🔍	pH 8	220 μl of Polyethylene glycol 20,000 (50 w/v) 100 μl of di-Potassium hydrogen phosphate pH 8 (1 M) 300 μl of Polyethylene glycol 400 (100 v/v) 380 μl of water (100 v/v)
B1	Polyethylene glycol 400 10 v/v - Polyethylene glycol monomethyl ether 5,000 20 w/v - 🔍	NA	400 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 100 μl of Polyethylene glycol 400 (100 v/v) 500 μl of water (100 v/v)
B2	Polyethylene glycol 400 34 v/v - HEPES 0.1 M pH 7.5 Sodium chloride 0.1 M - Cadmium chloride 0.2 M - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 200 μl of Cadmium chloride (1 M) 340 μl of Polyethylene glycol 400 (100 v/v) 100 μl of Sodium chloride (1 M) 260 μl of water (100 v/v)
B3	Polyethylene glycol 400 40 v/v - Polyethylene glycol 3,000 5 w/v - HEPES 0.1 M pH 6.5 🔍	pH 6.5	100 μl of Polyethylene glycol 3,000 (50 w/v) 100 μl of HEPES pH 6.5 (1 M) 400 μl of Polyethylene glycol 400 (100 v/v) 400 μl of water (100 v/v)
B4	Polyethylene glycol 600 35 w/v - PIPES 0.1 M pH 7 Sodium chloride 0.1 M - 🔍	pH 7	100 μl of PIPES pH 7 (1 M) 700 μl of Polyethylene glycol 600 (50 w/v) 100 μl of Sodium chloride (1 M) 100 μl of water (100 v/v)
B5	Polyethylene glycol 1,000 30 w/v - Sodium phosphate citrate 0.1 M pH 4.4 Lithium sulfate 0.11 M - 🔍	pH 4.4	36 μl of Lithium sulfate (3 M) 100 μl of Sodium phosphate citrate pH 4.4 (1 M) 600 μl of Polyethylene glycol 1,000 (50 w/v) 263 μl of water (100 v/v)
B6	Polyethylene glycol 1,500 40 w/v - ADA 0.1 M pH 6 🔍	pH 6	100 μl of ADA pH 6 (1 M) 800 μl of Polyethylene glycol 1,500 (50 w/v) 100 μl of water (100 v/v)
C1	Polyethylene glycol 1,500 17 w/v - 2-methyl-2,4-pentanediol BCC 10 v/v - PIPES 0.05 M pH 7 🔍	pH 7	50 μl of PIPES pH 7 (1 M) 100 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 340 μl of Polyethylene glycol 1,500 (50 w/v) 510 μl of water (100 v/v)
C2	Polyethylene glycol 3,350 35 w/v - Potassium thiocyanate 0.3 M - 🔍	NA	37 μl of Potassium thiocyanate (8 M) 700 μl of Polyethylene glycol 3,350 (50 w/v) 262 μl of water (100 v/v)
C3	Polyethylene glycol 4,000 25 w/v - 2-methyl-2,4-pentanediol BCC 18 v/v - Sodium acetate 0.1 M pH 4.6 Ammonium sulfate 0.2 M - 🔍	pH 4.6	66 μl of Ammonium sulfate (3 M) 500 μl of Polyethylene glycol 4,000 (50 w/v) 180 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100 μl of Sodium acetate pH 4.6 (1 M) 153 μl of water (100 v/v)
C4	Polyethylene glycol 4,000 25 w/v - Ethylene glycol 30 v/v - 🔍	NA	300 μl of Ethylene glycol (100 v/v) 500 μl of Polyethylene glycol 4,000 (50 w/v) 200 μl of water (100 v/v)
C5	Polyethylene glycol 4,000 20 w/v - 2-methyl-2,4-pentanediol BCC 20 v/v - 2-Propanol 10 v/v - tri-Sodium citrate 0.1 M pH 5.6 🔍	pH 5.6	100 μl of 2-Propanol (100 v/v) 400 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of tri-Sodium citrate pH 5.6 (1 M) 200 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 200 μl of water (100 v/v)
C6	Polyethylene glycol 4,000 10 w/v - Ethylene glycol 20 v/v - ADA 0.05 M pH 6 Sodium chloride 0.105 M - 🔍	pH 6	200 μl of Ethylene glycol (100 v/v) 200 μl of Polyethylene glycol 4,000 (50 w/v) 50 μl of ADA pH 6 (1 M) 105 μl of Sodium chloride (1 M) 445 μl of water (100 v/v)
D1	Polyethylene glycol 4,000 2 w/v - 2-methyl-2,4-pentanediol BCC 25 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 40 μl of Polyethylene glycol 4,000 (50 w/v) 250 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 610 μl of water (100 v/v)
D2	Polyethylene glycol 8,000 20 w/v - 2-methyl-2,4-pentanediol BCC 20 v/v - HEPES 0.1 M pH 7.5 Rubidium bromide 0.2 M - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 400 μl of Polyethylene glycol 8,000 (50 w/v) 200 μl of Rubidium bromide (1 M) 200 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100 μl of water (100 v/v)
D3	Polyethylene glycol 8,000 28 w/v - Glycine 0.1 M pH 9.5 Calcium chloride 0.01 M - 🔍	pH 9.5	10 μl of Calcium chloride (1 M) 50 μl of Glycine pH 9.5 (2 M) 560 μl of Polyethylene glycol 8,000 (50 w/v) 380 μl of water (100 v/v)
D4	Polyethylene glycol 8,000 10 w/v - 2-methyl-2,4-pentanediol BCC 15 v/v - MES 0.1 M pH 6.5 Ammonium sulfate 0.55 M - 🔍	pH 6.5	183 μl of Ammonium sulfate (3 M) 200 μl of Polyethylene glycol 8,000 (50 w/v) 33 μl of MES pH 6.5 (3 M) 150 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 433 μl of water (100 v/v)
D5	Polyethylene glycol 8,000 25 w/v - Ethylene glycol 30 v/v - ADA 0.15 M pH 6 Ammonium sulfate 0.08 M - 🔍	pH 6	26 μl of Ammonium sulfate (3 M) 300 μl of Ethylene glycol (100 v/v) 500 μl of Polyethylene glycol 8,000 (50 w/v) 150 μl of ADA pH 6 (1 M) 23 μl of water (100 v/v)
D6	Polyethylene glycol 20,000 10 w/v - Ethylene glycol 5.6 v/v - 2,3-Butanediol 30 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 56 μl of Ethylene glycol (100 v/v) 300 μl of 2,3-Butanediol (100 v/v) 200 μl of Polyethylene glycol 20,000 (50 w/v) 344 μl of water (100 v/v)