C6 - Screen Contents

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Screen Description

Cryo 4

Cryos / Cryo 4

Created 2009-11-16.

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Cryo 4

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Cryo 4

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Screen Structure

Cryo 4

Well Count	24
Number of distinct ingredients	21
Internal diversity (score mean dist)	0.902

Screen Composition

More information about screen composition chart.

ingredient type	Cryo 4
Buffer	29
Organic (non-volatile)	17
Organic (volatile)	3
Salt	24

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Cryo 4 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Ethylene glycol 20 v/v - HEPES 0.1 M pH 7.5 Lithium sulfate 1.6 M - 🔍	pH 7.5	200.0 μl of Ethylene glycol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 533.3 μl of Lithium sulfate (3 M) 166.7 μl of water (100 v/v)
A2	Ethylene glycol 20 v/v - HEPES 0.1 M pH 7.5 Sodium Potassium phosphate 0.2 M - 🔍	pH 7.5	200.0 μl of Ethylene glycol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Sodium Potassium phosphate pH 7 (1 M) 500.0 μl of water (100 v/v)
A3	Ethylene glycol 20 v/v - HEPES 0.1 M pH 7.5 Potassium Sodium tartrate 1 M - 🔍	pH 7.5	200.0 μl of Ethylene glycol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 500.0 μl of Potassium Sodium tartrate (2 M) 200.0 μl of water (100 v/v)
A4	Ethylene glycol 20 v/v - Sodium acetate 0.1 M pH 4.6 🔍	pH 4.6	200.0 μl of Ethylene glycol (100 v/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 700.0 μl of water (100 v/v)
A5	Ethylene glycol 40 v/v - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	400.0 μl of Ethylene glycol (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 500.0 μl of water (100 v/v)
A6	Ethylene glycol 40 v/v - Imidazole 0.1 M pH 9 Calcium acetate 0.2 M - 🔍	pH 9	100.0 μl of Calcium acetate (2 M) 400.0 μl of Ethylene glycol (100 v/v) 100.0 μl of Imidazole pH 9 (1 M) 400.0 μl of water (100 v/v)
B1	Ethylene glycol 40 v/v - MES 0.1 M pH 6.5 Zinc acetate 0.2 M - 🔍	pH 6.5	400.0 μl of Ethylene glycol (100 v/v) 33.3 μl of MES pH 6.5 (3 M) 100.0 μl of Zinc acetate (2 M) 466.7 μl of water (100 v/v)
B2	Ethylene glycol 20 v/v - tris chloride BCC 0.1 M pH 8.5 Magnesium chloride 0.2 M - 🔍	pH 8.5	200.0 μl of Ethylene glycol (100 v/v) 80.0 μl of Magnesium chloride pH 7 (2.5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 620.0 μl of water (100 v/v)
B3	Ethylene glycol 20 v/v - HEPES 0.1 M pH 7.5 Lithium sulfate 0.2 M - 🔍	pH 7.5	200.0 μl of Ethylene glycol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 66.7 μl of Lithium sulfate (3 M) 633.3 μl of water (100 v/v)
B4	2-Propanol 35 v/v - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	350.0 μl of 2-Propanol (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 550.0 μl of water (100 v/v)
B5	2-Propanol 35 v/v - Sodium acetate 0.1 M pH 4.6 🔍	pH 4.6	350.0 μl of 2-Propanol (100 v/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 550.0 μl of water (100 v/v)
B6	2-Propanol 35 v/v - Imidazole 0.1 M pH 9 Zinc acetate 0.2 M - 🔍	pH 9	350.0 μl of 2-Propanol (100 v/v) 100.0 μl of Imidazole pH 9 (1 M) 100.0 μl of Zinc acetate (2 M) 450.0 μl of water (100 v/v)
C1	1,2-Propanediol 40 v/v - Imidazole 0.1 M pH 9 🔍	pH 9	400.0 μl of 1,2-Propanediol (100 v/v) 100.0 μl of Imidazole pH 9 (1 M) 500.0 μl of water (100 v/v)
C2	1,2-Propanediol 40 v/v - Sodium acetate 0.1 M pH 4.6 🔍	pH 4.6	400.0 μl of 1,2-Propanediol (100 v/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 500.0 μl of water (100 v/v)
C3	1,2-Propanediol 40 v/v - tri-Sodium citrate 0.1 M pH 5.6 Sodium chloride 0.2 M - 🔍	pH 5.6	400.0 μl of 1,2-Propanediol (100 v/v) 40.0 μl of Sodium chloride (5 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 460.0 μl of water (100 v/v)
C4	1,2-Propanediol 40 v/v - Sodium acetate 0.1 M pH 4.6 Calcium acetate 0.5 M - 🔍	pH 4.6	400.0 μl of 1,2-Propanediol (100 v/v) 250.0 μl of Calcium acetate (2 M) 100.0 μl of Sodium acetate pH 4.6 (1 M) 250.0 μl of water (100 v/v)
C5	2-methyl-2,4-pentanediol BCC 35 v/v - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 550.0 μl of water (100 v/v)
C6	2-methyl-2,4-pentanediol BCC 30 v/v - Sodium acetate 0.1 M pH 4.6 Calcium chloride 0.2 M - 🔍	pH 4.6	300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 40.0 μl of Calcium chloride (5 M) 100.0 μl of Sodium acetate pH 4.6 (1 M) 560.0 μl of water (100 v/v)
D1	2-methyl-2,4-pentanediol BCC 30 v/v - HEPES 0.1 M pH 7.5 tri-Sodium citrate 0.2 M - 🔍	pH 7.5	300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 40.0 μl of tri-Sodium citrate (5 M) 560.0 μl of water (100 v/v)
D2	2-methyl-2,4-pentanediol BCC 30 v/v - tri-Sodium citrate 0.1 M pH 5.6 Ammonium acetate 0.2 M - 🔍	pH 5.6	300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 40.0 μl of Ammonium acetate (5 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 560.0 μl of water (100 v/v)
D3	2-methyl-2,4-pentanediol BCC 35 v/v - MES 0.1 M pH 6.5 Ammonium sulfate 0.2 M - 🔍	pH 6.5	350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 66.7 μl of Ammonium sulfate (3 M) 33.3 μl of MES pH 6.5 (3 M) 550.0 μl of water (100 v/v)
D4	2-methyl-2,4-pentanediol BCC 40 v/v - tri-Sodium citrate 0.1 M pH 5.6 🔍	pH 5.6	400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 500.0 μl of water (100 v/v)
D5	2-methyl-2,4-pentanediol BCC 40 v/v - MES 0.1 M pH 6.5 Ammonium sulfate 0.2 M - 🔍	pH 6.5	400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 66.7 μl of Ammonium sulfate (3 M) 33.3 μl of MES pH 6.5 (3 M) 500.0 μl of water (100 v/v)
D6	2-methyl-2,4-pentanediol BCC 40 v/v - Imidazole 0.1 M pH 9 Magnesium chloride 0.2 M - 🔍	pH 9	400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of Imidazole pH 9 (1 M) 80.0 μl of Magnesium chloride pH 7 (2.5 M) 420.0 μl of water (100 v/v)