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Supplemental Information

Screen Description

JCSG+ 1

JCSG / JCSG+ 1
Created 2009-11-16.

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JCSG+ 1

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Screen Structure

JCSG+ 1

Well Count 24
Number of distinct ingredients 33
Internal diversity (score mean dist) 0.949

Screen Composition

2025-08-16T04:27:16.102634 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type JCSG+ 1
Buffer 23
Organic (non-volatile) 5
Organic (volatile) 1
Polymer 20
Salt 22

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
JCSG+ 1 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Polyethylene glycol 400 50 v/v -
  • Sodium acetate 0.1 M pH 4.5
  • Lithium sulfate 0.2 M -
pH 4.5
80.0 μl of Lithium sulfate (2.5 M)
500.0 μl of Polyethylene glycol 400 (100 v/v)
50.0 μl of Sodium acetate pH 4.5 (2 M)
370.0 μl of water (100 v/v)
A2
  • Polyethylene glycol 3,000 20 w/v -
  • tri-Sodium citrate 0.1 M pH 5.5
pH 5.5
400.0 μl of Polyethylene glycol 3,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
500.0 μl of water (100 v/v)
A3
  • Polyethylene glycol 3,350 20 w/v -
  • di-Ammonium hydrogen citrate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
80.0 μl of di-Ammonium hydrogen citrate pH 5 (2.5 M)
520.0 μl of water (100 v/v)
A4
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
  • Sodium acetate 0.1 M pH 4.6
  • Calcium chloride 0.02 M -
pH 4.6
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
4.0 μl of Calcium chloride (5 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
596.0 μl of water (100 v/v)
A5
  • Polyethylene glycol 3,350 20 w/v -
  • Magnesium formate 0.2 M -
NA
200.0 μl of Magnesium formate pH 5.9 (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
A6
  • Potassium phosphate citrate 0.1 M pH 4.2
  • Polyethylene glycol 1,000 20 w/v -
  • Lithium sulfate 0.2 M -
pH 4.2
80.0 μl of Lithium sulfate (2.5 M)
400.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of Potassium phosphate citrate pH 4.2 (1 M)
420.0 μl of water (100 v/v)
B1
  • Polyethylene glycol 8,000 20 w/v -
  • CHES 0.1 M pH 9.5
pH 9.5
100.0 μl of CHES pH 9.5 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
500.0 μl of water (100 v/v)
B2
  • Polyethylene glycol 3,350 20 w/v -
  • Ammonium formate 0.2 M -
NA
40.0 μl of Ammonium formate pH 6.6 (5 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
560.0 μl of water (100 v/v)
B3
  • Polyethylene glycol 3,350 20 w/v -
  • Ammonium chloride 0.2 M -
NA
40.0 μl of Ammonium chloride pH 6.3 (5 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
560.0 μl of water (100 v/v)
B4
  • Polyethylene glycol 3,350 20 w/v -
  • Potassium formate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
14.3 μl of Potassium formate pH 7.3 (14 M)
585.7 μl of water (100 v/v)
B5
  • 2-methyl-2,4-pentanediol BCC 50 v/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium di-hydrogen phosphate 0.2 M -
pH 8.5
500.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
200.0 μl of Ammonium di-hydrogen phosphate (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
200.0 μl of water (100 v/v)
B6
  • Polyethylene glycol 3,350 20 w/v -
  • Potassium nitrate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
66.7 μl of Potassium nitrate pH 6.9 (3 M)
533.3 μl of water (100 v/v)
C1
  • tri-Sodium citrate 0.1 M pH 4
  • Ammonium sulfate 0.8 M -
pH 4
266.7 μl of Ammonium sulfate (3 M)
100.0 μl of tri-Sodium citrate pH 4 (1 M)
633.3 μl of water (100 v/v)
C2
  • Polyethylene glycol 3,350 20 w/v -
  • Sodium thiocyanate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
25.0 μl of Sodium thiocyanate pH 6.9 (8 M)
575.0 μl of water (100 v/v)
C3
  • Polyethylene glycol 6,000 20 w/v -
  • BICINE 0.1 M pH 9
pH 9
100.0 μl of BICINE pH 9 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
500.0 μl of water (100 v/v)
C4
  • Polyethylene glycol 8,000 10 w/v -
  • Ethylene glycol 8 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
80.0 μl of Ethylene glycol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
620.0 μl of water (100 v/v)
C5
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
  • Polyethylene glycol 8,000 5 w/v -
  • MES 0.1 M pH 6.5
pH 6.5
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of MES pH 6.5 (1 M)
100.0 μl of Polyethylene glycol 8,000 (50 w/v)
400.0 μl of water (100 v/v)
C6
  • Ethanol 40 v/v -
  • Polyethylene glycol 1,000 5 w/v -
  • Potassium phosphate citrate 0.1 M pH 4.2
pH 4.2
800.0 μl of Ethanol (50 v/v)
100.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of Potassium phosphate citrate pH 4.2 (1 M)
D1
  • Polyethylene glycol 4,000 8 w/v -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
160.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
740.0 μl of water (100 v/v)
D2
  • Polyethylene glycol 8,000 10 w/v -
  • tris chloride BCC 0.1 M pH 7
  • Magnesium chloride 0.2 M -
pH 7
80.0 μl of Magnesium chloride (2.5 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
33.3 μl of tris chloride BCC pH 7 (3 M)
686.7 μl of water (100 v/v)
D3
  • Polyethylene glycol 6,000 20 w/v -
  • tri-Sodium citrate 0.1 M pH 5
pH 5
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
500.0 μl of water (100 v/v)
D4
  • Polyethylene glycol 200 50 v/v -
  • MES 0.1 M pH 6.5
  • Magnesium chloride 0.2 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
500.0 μl of Polyethylene glycol 200 (100 v/v)
320.0 μl of water (100 v/v)
D5
  • tri-Sodium citrate 1.6 M -
NA
800.0 μl of tri-Sodium citrate (2 M)
200.0 μl of water (100 v/v)
D6
  • Polyethylene glycol 3,350 20 w/v -
  • tri-Potassium citrate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
80.0 μl of tri-Potassium citrate pH 8.3 (2.5 M)
520.0 μl of water (100 v/v)