C6 - JCSG+ 1 - Screen Contents

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Supplemental Information

Screen Description

JCSG / JCSG+ 1

Created 2009-11-16.

Rigaku Recipe XML

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Screen Structure

Well Count	24
Number of distinct ingredients	33
Internal diversity (score mean dist)	0.949

Screen Composition

More information about screen composition chart.

ingredient type	JCSG+ 1
Buffer	23
Organic (non-volatile)	5
Organic (volatile)	1
Polymer	20
Salt	22

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

JCSG+ 1 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Polyethylene glycol 400 50 v/v - Sodium acetate 0.1 M pH 4.5 Lithium sulfate 0.2 M - 🔍	pH 4.5	80.0 μl of Lithium sulfate (2.5 M) 500.0 μl of Polyethylene glycol 400 (100 v/v) 50.0 μl of Sodium acetate pH 4.5 (2 M) 370.0 μl of water (100 v/v)
A2	Polyethylene glycol 3,000 20 w/v - tri-Sodium citrate 0.1 M pH 5.5 🔍	pH 5.5	400.0 μl of Polyethylene glycol 3,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5.5 (1 M) 500.0 μl of water (100 v/v)
A3	Polyethylene glycol 3,350 20 w/v - di-Ammonium hydrogen citrate 0.2 M - 🔍	NA	80.0 μl of di-Ammonium hydrogen citrate pH 5 (2.5 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 520.0 μl of water (100 v/v)
A4	2-methyl-2,4-pentanediol BCC 30 v/v - Sodium acetate 0.1 M pH 4.6 Calcium chloride 0.02 M - 🔍	pH 4.6	300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 4.0 μl of Calcium chloride (5 M) 100.0 μl of Sodium acetate pH 4.6 (1 M) 596.0 μl of water (100 v/v)
A5	Polyethylene glycol 3,350 20 w/v - Magnesium formate 0.2 M - 🔍	NA	200.0 μl of Magnesium formate pH 5.9 (1 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 400.0 μl of water (100 v/v)
A6	Potassium phosphate citrate 0.1 M pH 4.2 Polyethylene glycol 1,000 20 w/v - Lithium sulfate 0.2 M - 🔍	pH 4.2	80.0 μl of Lithium sulfate (2.5 M) 400.0 μl of Polyethylene glycol 1,000 (50 w/v) 100.0 μl of Potassium phosphate citrate pH 4.2 (1 M) 420.0 μl of water (100 v/v)
B1	Polyethylene glycol 8,000 20 w/v - CHES 0.1 M pH 9.5 🔍	pH 9.5	100.0 μl of CHES pH 9.5 (1 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 500.0 μl of water (100 v/v)
B2	Polyethylene glycol 3,350 20 w/v - Ammonium formate 0.2 M - 🔍	NA	40.0 μl of Ammonium formate pH 6.6 (5 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 560.0 μl of water (100 v/v)
B3	Polyethylene glycol 3,350 20 w/v - Ammonium chloride 0.2 M - 🔍	NA	40.0 μl of Ammonium chloride pH 6.3 (5 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 560.0 μl of water (100 v/v)
B4	Polyethylene glycol 3,350 20 w/v - Potassium formate 0.2 M - 🔍	NA	400.0 μl of Polyethylene glycol 3,350 (50 w/v) 14.3 μl of Potassium formate pH 7.3 (14 M) 585.7 μl of water (100 v/v)
B5	2-methyl-2,4-pentanediol BCC 50 v/v - tris chloride BCC 0.1 M pH 8.5 Ammonium di-hydrogen phosphate 0.2 M - 🔍	pH 8.5	500.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 200.0 μl of Ammonium di-hydrogen phosphate (1 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 200.0 μl of water (100 v/v)
B6	Polyethylene glycol 3,350 20 w/v - Potassium nitrate 0.2 M - 🔍	NA	400.0 μl of Polyethylene glycol 3,350 (50 w/v) 66.7 μl of Potassium nitrate pH 6.9 (3 M) 533.3 μl of water (100 v/v)
C1	tri-Sodium citrate 0.1 M pH 4 Ammonium sulfate 0.8 M - 🔍	pH 4	266.7 μl of Ammonium sulfate (3 M) 100.0 μl of tri-Sodium citrate pH 4 (1 M) 633.3 μl of water (100 v/v)
C2	Polyethylene glycol 3,350 20 w/v - Sodium thiocyanate 0.2 M - 🔍	NA	400.0 μl of Polyethylene glycol 3,350 (50 w/v) 25.0 μl of Sodium thiocyanate pH 6.9 (8 M) 575.0 μl of water (100 v/v)
C3	Polyethylene glycol 6,000 20 w/v - BICINE 0.1 M pH 9 🔍	pH 9	100.0 μl of BICINE pH 9 (1 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 500.0 μl of water (100 v/v)
C4	Polyethylene glycol 8,000 10 w/v - Ethylene glycol 8 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	80.0 μl of Ethylene glycol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 620.0 μl of water (100 v/v)
C5	2-methyl-2,4-pentanediol BCC 40 v/v - Polyethylene glycol 8,000 5 w/v - MES 0.1 M pH 6.5 🔍	pH 6.5	400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of MES pH 6.5 (1 M) 100.0 μl of Polyethylene glycol 8,000 (50 w/v) 400.0 μl of water (100 v/v)
C6	Ethanol 40 v/v - Polyethylene glycol 1,000 5 w/v - Potassium phosphate citrate 0.1 M pH 4.2 🔍	pH 4.2	800.0 μl of Ethanol (50 v/v) 100.0 μl of Polyethylene glycol 1,000 (50 w/v) 100.0 μl of Potassium phosphate citrate pH 4.2 (1 M)
D1	Polyethylene glycol 4,000 8 w/v - Sodium acetate 0.1 M pH 4.6 🔍	pH 4.6	160.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 740.0 μl of water (100 v/v)
D2	Polyethylene glycol 8,000 10 w/v - tris chloride BCC 0.1 M pH 7 Magnesium chloride 0.2 M - 🔍	pH 7	80.0 μl of Magnesium chloride (2.5 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 33.3 μl of tris chloride BCC pH 7 (3 M) 686.7 μl of water (100 v/v)
D3	Polyethylene glycol 6,000 20 w/v - tri-Sodium citrate 0.1 M pH 5 🔍	pH 5	400.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 500.0 μl of water (100 v/v)
D4	Polyethylene glycol 200 50 v/v - MES 0.1 M pH 6.5 Magnesium chloride 0.2 M - 🔍	pH 6.5	80.0 μl of Magnesium chloride (2.5 M) 100.0 μl of MES pH 6.5 (1 M) 500.0 μl of Polyethylene glycol 200 (100 v/v) 320.0 μl of water (100 v/v)
D5	tri-Sodium citrate 1.6 M - 🔍	NA	800.0 μl of tri-Sodium citrate (2 M) 200.0 μl of water (100 v/v)
D6	Polyethylene glycol 3,350 20 w/v - tri-Potassium citrate 0.2 M - 🔍	NA	400.0 μl of Polyethylene glycol 3,350 (50 w/v) 80.0 μl of tri-Potassium citrate pH 8.3 (2.5 M) 520.0 μl of water (100 v/v)