C6 - Screen Contents

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Supplemental Information

Screen Description

JCSG+ 2

JCSG / JCSG+ 2

Created 2009-11-16.

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JCSG+ 2

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JCSG+ 2

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Screen Structure

JCSG+ 2

Well Count	24
Number of distinct ingredients	36
Internal diversity (score mean dist)	0.963

Screen Composition

More information about screen composition chart.

ingredient type	JCSG+ 2
Buffer	23
Cryoprotectant	5
Organic (non-volatile)	9
Organic (volatile)	3
Polymer	18
Salt	23

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

JCSG+ 2 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Polyethylene glycol 8,000 20 w/v - Potassium phosphate citrate 0.1 M pH 4.2 Sodium chloride 0.2 M - 🔍	pH 4.2	400.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Potassium phosphate citrate pH 4.2 (1 M) 200.0 μl of Sodium chloride (1 M) 300.0 μl of water (100 v/v)
A2	Polyethylene glycol 6,000 20 w/v - tri-Sodium citrate 0.1 M pH 4 Lithium chloride 1 M - 🔍	pH 4	200.0 μl of Lithium chloride (5 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 4 (1 M) 300.0 μl of water (100 v/v)
A3	Polyethylene glycol 3,350 20 w/v - Ammonium nitrate 0.2 M - 🔍	NA	20.0 μl of Ammonium nitrate pH 6.3 (10 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 580.0 μl of water (100 v/v)
A4	Polyethylene glycol 6,000 10 w/v - HEPES 0.1 M pH 7 🔍	pH 7	100.0 μl of HEPES pH 7 (1 M) 200.0 μl of Polyethylene glycol 6,000 (50 w/v) 700.0 μl of water (100 v/v)
A5	Sodium di-hydrogen phosphate 0.8 M - Potassium di-hydrogen phosphate 0.8 M - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Potassium di-hydrogen phosphate (2 M) 200.0 μl of Sodium di-hydrogen phosphate (4 M) 300.0 μl of water (100 v/v)
A6	Polyethylene glycol 300 40 v/v - Potassium phosphate citrate 0.1 M pH 4.2 🔍	pH 4.2	400.0 μl of Polyethylene glycol 300 (100 v/v) 100.0 μl of Potassium phosphate citrate pH 4.2 (1 M) 500.0 μl of water (100 v/v)
B1	Polyethylene glycol 3,000 10 w/v - Sodium acetate 0.1 M pH 4.5 Zinc acetate 0.2 M - 🔍	pH 4.5	200.0 μl of Polyethylene glycol 3,000 (50 w/v) 50.0 μl of Sodium acetate pH 4.5 (2 M) 80.0 μl of Zinc acetate (2.5 M) 670.0 μl of water (100 v/v)
B2	Ethanol 20 v/v - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	400.0 μl of Ethanol (50 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 500.0 μl of water (100 v/v)
B3	1,2-Propanediol 25 v/v - Glycerol 10 v/v - Sodium Potassium phosphate 0.1 M pH 6.2 🔍	pH 6.2	250.0 μl of 1,2-Propanediol (100 v/v) 100.0 μl of Glycerol (100 v/v) 25.0 μl of Sodium Potassium phosphate pH 6.2 (4 M) 625.0 μl of water (100 v/v)
B4	Polyethylene glycol 20,000 10 w/v - BICINE 0.1 M pH 9 1,4-Dioxane 2 v/v - 🔍	pH 9	40.0 μl of 1,4-Dioxane (50 v/v) 100.0 μl of BICINE pH 9 (1 M) 200.0 μl of Polyethylene glycol 20,000 (50 w/v) 660.0 μl of water (100 v/v)
B5	Sodium acetate 0.1 M pH 4.6 Ammonium sulfate 2 M - 🔍	pH 4.6	666.7 μl of Ammonium sulfate (3 M) 100.0 μl of Sodium acetate pH 4.6 (1 M) 233.3 μl of water (100 v/v)
B6	Polyethylene glycol 1,000 10 w/v - Polyethylene glycol 8,000 10 w/v - 🔍	NA	200.0 μl of Polyethylene glycol 1,000 (50 w/v) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 600.0 μl of water (100 v/v)
C1	Polyethylene glycol 1,500 24 w/v - Glycerol 20 v/v - 🔍	NA	200.0 μl of Glycerol (100 v/v) 480.0 μl of Polyethylene glycol 1,500 (50 w/v) 320.0 μl of water (100 v/v)
C2	Polyethylene glycol 400 30 v/v - HEPES 0.1 M pH 7.5 Magnesium chloride 0.2 M - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 80.0 μl of Magnesium chloride (2.5 M) 300.0 μl of Polyethylene glycol 400 (100 v/v) 520.0 μl of water (100 v/v)
C3	Polyethylene glycol 200 50 v/v - Sodium Potassium phosphate 0.1 M pH 6.2 Sodium chloride 0.2 M - 🔍	pH 6.2	500.0 μl of Polyethylene glycol 200 (100 v/v) 25.0 μl of Sodium Potassium phosphate pH 6.2 (4 M) 40.0 μl of Sodium chloride (5 M) 435.0 μl of water (100 v/v)
C4	Polyethylene glycol 8,000 30 w/v - Sodium acetate 0.1 M pH 4.5 Lithium sulfate 0.2 M - 🔍	pH 4.5	80.0 μl of Lithium sulfate (2.5 M) 600.0 μl of Polyethylene glycol 8,000 (50 w/v) 50.0 μl of Sodium acetate pH 4.5 (2 M) 270.0 μl of water (100 v/v)
C5	2-methyl-2,4-pentanediol BCC 70 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	700.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of water (100 v/v)
C6	Polyethylene glycol 8,000 20 w/v - tris chloride BCC 0.1 M pH 8.5 Magnesium chloride 0.2 M - 🔍	pH 8.5	80.0 μl of Magnesium chloride (2.5 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 420.0 μl of water (100 v/v)
D1	Polyethylene glycol 400 40 v/v - tris chloride BCC 0.1 M pH 8.5 Lithium sulfate 0.2 M - 🔍	pH 8.5	80.0 μl of Lithium sulfate (2.5 M) 400.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 420.0 μl of water (100 v/v)
D2	2-methyl-2,4-pentanediol BCC 40 v/v - tris chloride BCC 0.1 M pH 8 🔍	pH 8	400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of tris chloride BCC pH 8 (1 M) 500.0 μl of water (100 v/v)
D3	Polyethylene glycol 4,000 25.5 w/v - Glycerol 15 v/v - Ammonium sulfate 0.17 M - 🔍	NA	56.7 μl of Ammonium sulfate (3 M) 150.0 μl of Glycerol (100 v/v) 510.0 μl of Polyethylene glycol 4,000 (50 w/v) 283.3 μl of water (100 v/v)
D4	Polyethylene glycol 300 40 v/v - MES 0.1 M pH 6.5 Calcium acetate 0.2 M - 🔍	pH 6.5	100.0 μl of Calcium acetate (2 M) 33.3 μl of MES pH 6.5 (3 M) 400.0 μl of Polyethylene glycol 300 (100 v/v) 466.7 μl of water (100 v/v)
D5	2-Propanol 14 v/v - Glycerol 30 v/v - Sodium acetate 0.07 M pH 4.6 Calcium chloride 0.14 M - 🔍	pH 4.6	140.0 μl of 2-Propanol (100 v/v) 28.0 μl of Calcium chloride (5 M) 300.0 μl of Glycerol (100 v/v) 70.0 μl of Sodium acetate pH 4.6 (1 M) 462.0 μl of water (100 v/v)
D6	Polyethylene glycol 8,000 16 w/v - Glycerol 20 v/v - Potassium di-hydrogen phosphate 0.04 M - 🔍	NA	200.0 μl of Glycerol (100 v/v) 320.0 μl of Polyethylene glycol 8,000 (50 w/v) 20.0 μl of Potassium di-hydrogen phosphate (2 M) 460.0 μl of water (100 v/v)