C6 - Screen Contents

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Supplemental Information

Screen Description

JCSG+ 2

JCSG / JCSG+ 2

Created 2009-11-16.

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JCSG+ 2

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JCSG+ 2

Screen Structure

JCSG+ 2

Well Count	24
Number of distinct ingredients	36
Internal diversity (score mean dist)	0.963

Screen Composition

More information about screen composition chart.

ingredient type	JCSG+ 2
Buffer	23
Cryoprotectant	5
Organic (non-volatile)	9
Organic (volatile)	3
Polymer	18
Salt	23

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

JCSG+ 2 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Polyethylene glycol 8,000 20 w/v - Potassium phosphate citrate 0.1 M pH 4.2 Sodium chloride 0.2 M - 🔍	pH 4.2	100 μl of Potassium phosphate citrate pH 4.2 (1 M) 400 μl of Polyethylene glycol 8,000 (50 w/v) 200 μl of Sodium chloride (1 M) 300 μl of water (100 v/v)
A2	Polyethylene glycol 6,000 20 w/v - tri-Sodium citrate 0.1 M pH 4 Lithium chloride 1 M - 🔍	pH 4	400 μl of Polyethylene glycol 6,000 (50 w/v) 100 μl of tri-Sodium citrate pH 4 (1 M) 200 μl of Lithium chloride (5 M) 300 μl of water (100 v/v)
A3	Polyethylene glycol 3,350 20 w/v - Ammonium nitrate 0.2 M - 🔍	NA	20 μl of Ammonium nitrate pH 6.3 (10 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 580 μl of water (100 v/v)
A4	Polyethylene glycol 6,000 10 w/v - HEPES 0.1 M pH 7 🔍	pH 7	100 μl of HEPES pH 7 (1 M) 200 μl of Polyethylene glycol 6,000 (50 w/v) 700 μl of water (100 v/v)
A5	Sodium di-hydrogen phosphate 0.8 M - Potassium di-hydrogen phosphate 0.8 M - HEPES 0.1 M pH 7.5 🔍	pH 7.5	200 μl of Sodium di-hydrogen phosphate (4 M) 100 μl of HEPES pH 7.5 (1 M) 400 μl of Potassium di-hydrogen phosphate (2 M) 300 μl of water (100 v/v)
A6	Polyethylene glycol 300 40 v/v - Potassium phosphate citrate 0.1 M pH 4.2 🔍	pH 4.2	400 μl of Polyethylene glycol 300 (100 v/v) 100 μl of Potassium phosphate citrate pH 4.2 (1 M) 500 μl of water (100 v/v)
B1	Polyethylene glycol 3,000 10 w/v - Sodium acetate 0.1 M pH 4.5 Zinc acetate 0.2 M - 🔍	pH 4.5	50 μl of Sodium acetate pH 4.5 (2 M) 80 μl of Zinc acetate (2.5 M) 200 μl of Polyethylene glycol 3,000 (50 w/v) 670 μl of water (100 v/v)
B2	Ethanol 20 v/v - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	100 μl of tris chloride BCC pH 8.5 (1 M) 400 μl of Ethanol (50 v/v) 500 μl of water (100 v/v)
B3	1,2-Propanediol 25 v/v - Glycerol 10 v/v - Sodium Potassium phosphate 0.1 M pH 6.2 🔍	pH 6.2	250 μl of 1,2-Propanediol (100 v/v) 100 μl of Glycerol (100 v/v) 25 μl of Sodium Potassium phosphate pH 6.2 (4 M) 625 μl of water (100 v/v)
B4	Polyethylene glycol 20,000 10 w/v - BICINE 0.1 M pH 9 1,4-Dioxane 2 v/v - 🔍	pH 9	200 μl of Polyethylene glycol 20,000 (50 w/v) 100 μl of BICINE pH 9 (1 M) 40 μl of 1,4-Dioxane (50 v/v) 660 μl of water (100 v/v)
B5	Sodium acetate 0.1 M pH 4.6 Ammonium sulfate 2 M - 🔍	pH 4.6	100 μl of Sodium acetate pH 4.6 (1 M) 666 μl of Ammonium sulfate (3 M) 233 μl of water (100 v/v)
B6	Polyethylene glycol 1,000 10 w/v - Polyethylene glycol 8,000 10 w/v - 🔍	NA	200 μl of Polyethylene glycol 8,000 (50 w/v) 200 μl of Polyethylene glycol 1,000 (50 w/v) 600 μl of water (100 v/v)
C1	Polyethylene glycol 1,500 24 w/v - Glycerol 20 v/v - 🔍	NA	480 μl of Polyethylene glycol 1,500 (50 w/v) 200 μl of Glycerol (100 v/v) 320 μl of water (100 v/v)
C2	Polyethylene glycol 400 30 v/v - HEPES 0.1 M pH 7.5 Magnesium chloride 0.2 M - 🔍	pH 7.5	300 μl of Polyethylene glycol 400 (100 v/v) 80 μl of Magnesium chloride (2.5 M) 100 μl of HEPES pH 7.5 (1 M) 520 μl of water (100 v/v)
C3	Polyethylene glycol 200 50 v/v - Sodium Potassium phosphate 0.1 M pH 6.2 Sodium chloride 0.2 M - 🔍	pH 6.2	40 μl of Sodium chloride (5 M) 25 μl of Sodium Potassium phosphate pH 6.2 (4 M) 500 μl of Polyethylene glycol 200 (100 v/v) 435 μl of water (100 v/v)
C4	Polyethylene glycol 8,000 30 w/v - Sodium acetate 0.1 M pH 4.5 Lithium sulfate 0.2 M - 🔍	pH 4.5	50 μl of Sodium acetate pH 4.5 (2 M) 80 μl of Lithium sulfate (2.5 M) 600 μl of Polyethylene glycol 8,000 (50 w/v) 270 μl of water (100 v/v)
C5	2-methyl-2,4-pentanediol BCC 70 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 700 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 200 μl of water (100 v/v)
C6	Polyethylene glycol 8,000 20 w/v - tris chloride BCC 0.1 M pH 8.5 Magnesium chloride 0.2 M - 🔍	pH 8.5	100 μl of tris chloride BCC pH 8.5 (1 M) 400 μl of Polyethylene glycol 8,000 (50 w/v) 80 μl of Magnesium chloride (2.5 M) 420 μl of water (100 v/v)
D1	Polyethylene glycol 400 40 v/v - tris chloride BCC 0.1 M pH 8.5 Lithium sulfate 0.2 M - 🔍	pH 8.5	400 μl of Polyethylene glycol 400 (100 v/v) 80 μl of Lithium sulfate (2.5 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 420 μl of water (100 v/v)
D2	2-methyl-2,4-pentanediol BCC 40 v/v - tris chloride BCC 0.1 M pH 8 🔍	pH 8	100 μl of tris chloride BCC pH 8 (1 M) 400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 500 μl of water (100 v/v)
D3	Polyethylene glycol 4,000 25.5 w/v - Glycerol 15 v/v - Ammonium sulfate 0.17 M - 🔍	NA	150 μl of Glycerol (100 v/v) 510 μl of Polyethylene glycol 4,000 (50 w/v) 56 μl of Ammonium sulfate (3 M) 283 μl of water (100 v/v)
D4	Polyethylene glycol 300 40 v/v - MES 0.1 M pH 6.5 Calcium acetate 0.2 M - 🔍	pH 6.5	100 μl of Calcium acetate (2 M) 400 μl of Polyethylene glycol 300 (100 v/v) 33 μl of MES pH 6.5 (3 M) 466 μl of water (100 v/v)
D5	2-Propanol 14 v/v - Glycerol 30 v/v - Sodium acetate 0.07 M pH 4.6 Calcium chloride 0.14 M - 🔍	pH 4.6	70 μl of Sodium acetate pH 4.6 (1 M) 300 μl of Glycerol (100 v/v) 140 μl of 2-Propanol (100 v/v) 28 μl of Calcium chloride (5 M) 462 μl of water (100 v/v)
D6	Polyethylene glycol 8,000 16 w/v - Glycerol 20 v/v - Potassium di-hydrogen phosphate 0.04 M - 🔍	NA	200 μl of Glycerol (100 v/v) 320 μl of Polyethylene glycol 8,000 (50 w/v) 20 μl of Potassium di-hydrogen phosphate (2 M) 460 μl of water (100 v/v)