C6 - Screen Contents

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Supplemental Information

Screen Description

JCSG+ 3

JCSG / JCSG+ 3

Created 2009-11-16.

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JCSG+ 3

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JCSG+ 3

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Screen Structure

JCSG+ 3

Well Count	24
Number of distinct ingredients	31
Internal diversity (score mean dist)	0.960

Screen Composition

More information about screen composition chart.

ingredient type	JCSG+ 3
Buffer	28
Cryoprotectant	1
Organic (non-volatile)	8
Organic (volatile)	2
Polymer	9
Salt	21

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

JCSG+ 3 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	MES 0.1 M pH 6.5 tri-Sodium citrate 1 M - 🔍	pH 6.5	33.3 μl of MES pH 6.5 (3 M) 500.0 μl of tri-Sodium citrate (2 M) 466.7 μl of water (100 v/v)
A2	Ammonium sulfate 2 M - MES 0.1 M pH 6.5 Sodium chloride 0.2 M - 🔍	pH 6.5	400.0 μl of Ammonium sulfate (5 M) 33.3 μl of MES pH 6.5 (3 M) 40.0 μl of Sodium chloride (5 M) 526.7 μl of water (100 v/v)
A3	2-Propanol 10 v/v - HEPES 0.1 M pH 7.5 Sodium chloride 0.2 M - 🔍	pH 7.5	100.0 μl of 2-Propanol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 40.0 μl of Sodium chloride (5 M) 760.0 μl of water (100 v/v)
A4	Ammonium sulfate 1.26 M - tris chloride BCC 0.1 M pH 8.5 Lithium sulfate 0.2 M - 🔍	pH 8.5	420.0 μl of Ammonium sulfate (3 M) 80.0 μl of Lithium sulfate (2.5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 400.0 μl of water (100 v/v)
A5	2-methyl-2,4-pentanediol BCC 40 v/v - CAPS 0.1 M pH 10 🔍	pH 10	400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of CAPS pH 10 (1 M) 500.0 μl of water (100 v/v)
A6	Polyethylene glycol 3,000 20 w/v - Imidazole 0.1 M pH 8 Zinc acetate 0.2 M - 🔍	pH 8	100.0 μl of Imidazole pH 8 (1 M) 400.0 μl of Polyethylene glycol 3,000 (50 w/v) 100.0 μl of Zinc acetate (2 M) 400.0 μl of water (100 v/v)
B1	2-Propanol 10 v/v - MES 0.1 M pH 6.5 Zinc acetate 0.2 M - 🔍	pH 6.5	100.0 μl of 2-Propanol (100 v/v) 33.3 μl of MES pH 6.5 (3 M) 100.0 μl of Zinc acetate (2 M) 766.7 μl of water (100 v/v)
B2	Sodium acetate 0.1 M pH 4.5 di-Ammonium hydrogen phosphate 1 M - 🔍	pH 4.5	33.3 μl of Sodium acetate pH 4.5 (3 M) 285.7 μl of di-Ammonium hydrogen phosphate (3.5 M) 681.0 μl of water (100 v/v)
B3	MES 0.1 M pH 6.5 Magnesium sulfate 1.6 M - 🔍	pH 6.5	33.3 μl of MES pH 6.5 (3 M) 800.0 μl of Magnesium sulfate (2 M) 166.7 μl of water (100 v/v)
B4	Polyethylene glycol 6,000 10 w/v - BICINE 0.1 M pH 9 🔍	pH 9	100.0 μl of BICINE pH 9 (1 M) 200.0 μl of Polyethylene glycol 6,000 (50 w/v) 700.0 μl of water (100 v/v)
B5	Polyethylene glycol 8,000 14.4 w/v - Glycerol 20 v/v - MES 0.08 M pH 6.5 Calcium acetate 0.16 M - 🔍	pH 6.5	160.0 μl of Calcium acetate (1 M) 200.0 μl of Glycerol (100 v/v) 26.7 μl of MES pH 6.5 (3 M) 288.0 μl of Polyethylene glycol 8,000 (50 w/v) 325.3 μl of water (100 v/v)
B6	Polyethylene glycol 8,000 10 w/v - Imidazole 0.1 M pH 8 🔍	pH 8	100.0 μl of Imidazole pH 8 (1 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 700.0 μl of water (100 v/v)
C1	Jeffamine® M-600 30 v/v - MES 0.1 M pH 6.5 Cesium chloride 0.05 M - 🔍	pH 6.5	50.0 μl of Cesium chloride (1 M) 600.0 μl of Jeffamine® M-600 (50 v/v) 33.3 μl of MES pH 6.5 (3 M) 316.7 μl of water (100 v/v)
C2	tri-Sodium citrate 0.1 M pH 5 Ammonium sulfate 3.15 M - 🔍	pH 5	787.5 μl of Ammonium sulfate (4 M) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 112.5 μl of water (100 v/v)
C3	2-methyl-2,4-pentanediol BCC 20 v/v - tris chloride BCC 0.1 M pH 8 🔍	pH 8	200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of tris chloride BCC pH 8 (1 M) 700.0 μl of water (100 v/v)
C4	Jeffamine® M-600 20 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Jeffamine® M-600 (50 v/v) 500.0 μl of water (100 v/v)
C5	Ethylene glycol 50 v/v - tris chloride BCC 0.1 M pH 8.5 Magnesium chloride 0.2 M - 🔍	pH 8.5	500.0 μl of Ethylene glycol (100 v/v) 80.0 μl of Magnesium chloride (2.5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 320.0 μl of water (100 v/v)
C6	2-methyl-2,4-pentanediol BCC 10 v/v - BICINE 0.1 M pH 9 🔍	pH 9	100.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of BICINE pH 9 (1 M) 800.0 μl of water (100 v/v)
D1	di-Sodium succinate 0.8 M - 🔍	NA	266.7 μl of di-Sodium succinate pH 7 (3 M) 733.3 μl of water (100 v/v)
D2	di-Sodium DL-malate 2.1 M - 🔍	NA	700.0 μl of di-Sodium DL-malate pH 7 (3 M) 300.0 μl of water (100 v/v)
D3	di-Sodium malonate 2.4 M - 🔍	NA	800.0 μl of di-Sodium malonate pH 7 (3 M) 200.0 μl of water (100 v/v)
D4	di-Sodium malonate 1.1 M - Jeffamine® ED-2001 0.5 w/v - HEPES 0.1 M pH 7 🔍	pH 7	100.0 μl of HEPES pH 7 (1 M) 10.0 μl of Jeffamine® ED-2001 pH 7 (50 w/v) 366.7 μl of di-Sodium malonate pH 7 (3 M) 523.3 μl of water (100 v/v)
D5	Polyethylene glycol monomethyl ether 2,000 1 w/v - HEPES 0.1 M pH 7 di-Sodium succinate 1 M - 🔍	pH 7	100.0 μl of HEPES pH 7 (1 M) 20.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 333.3 μl of di-Sodium succinate pH 7 (3 M) 546.7 μl of water (100 v/v)
D6	Jeffamine® M-600 30 v/v - HEPES 0.1 M pH 7 🔍	pH 7	100.0 μl of HEPES pH 7 (1 M) 600.0 μl of Jeffamine® M-600 pH 7 (50 v/v) 300.0 μl of water (100 v/v)