C6 - JCSG+ 3 - Screen Contents

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Supplemental Information

Screen Description

JCSG / JCSG+ 3

Created 2009-11-16.

Rigaku Recipe XML

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Screen Structure

Well Count	24
Number of distinct ingredients	31
Internal diversity (score mean dist)	0.960

Screen Composition

More information about screen composition chart.

ingredient type	JCSG+ 3
Buffer	28
Cryoprotectant	1
Organic (non-volatile)	8
Organic (volatile)	2
Polymer	9
Salt	21

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

JCSG+ 3 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	MES 0.1 M pH 6.5 tri-Sodium citrate 1 M - 🔍	pH 6.5	27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 1000.0 μl of trisodium citrate BCC (1 M)
A2	Ammonium sulfate 2 M - MES 0.1 M pH 6.5 Sodium chloride 0.2 M - 🔍	pH 6.5	2000.0 μl of ammonium sulfate BCC (1 M) 200.0 μl of sodium chloride BCC (1 M) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M)
A3	2-Propanol 10 v/v - HEPES 0.1 M pH 7.5 Sodium chloride 0.2 M - 🔍	pH 7.5	125.0 μl of 2-propanol BCC (80 v/v) 200.0 μl of sodium chloride BCC (1 M) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 575.0 μl of water (100 v/v)
A4	Ammonium sulfate 1.26 M - tris chloride BCC 0.1 M pH 8.5 Lithium sulfate 0.2 M - 🔍	pH 8.5	1260.0 μl of ammonium sulfate BCC (1 M) 200.0 μl of lithium sulfate BCC (1 M) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M)
A5	2-methyl-2,4-pentanediol BCC 40 v/v - CAPS 0.1 M pH 10.5 🔍	pH 10.5	400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 86.0 μl of CAPS BCC pH 9.4 (0.5 M) 114.0 μl of CAPS BCC pH 11.4 (0.5 M) 400.0 μl of water (100 v/v)
A6	Polyethylene glycol 3,000 20 w/v - Imidazole 0.1 M pH 8 Zinc acetate 0.2 M - 🔍	pH 8	100.0 μl of imidazole BCC pH 8 (1 M) 400.0 μl of polyethylene glycol 3000 BCC (50 w/v) 200.0 μl of zinc acetate BCC (1 M) 300.0 μl of water (100 v/v)
B1	2-Propanol 10 v/v - MES 0.1 M pH 6.5 Zinc acetate 0.2 M - 🔍	pH 6.5	125.0 μl of 2-propanol BCC (80 v/v) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 200.0 μl of zinc acetate BCC (1 M) 575.0 μl of water (100 v/v)
B2	Sodium acetate 0.1 M pH 4.5 di-Ammonium hydrogen phosphate 1 M - 🔍	pH 4.5	285.7 μl of diammonium hydrogen phosphate BCC (3.5 M) 31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 614.3 μl of water (100 v/v)
B3	MES 0.1 M pH 6.5 Magnesium sulfate 1.6 M - 🔍	pH 6.5	1600.0 μl of magnesium sulfate BCC (1 M) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M)
B4	Polyethylene glycol 6,000 10 w/v - BICINE 0.1 M pH 9 🔍	pH 9	500.0 μl of polyethylene glycol 6000 BCC (20 w/v) 12.4 μl of sodium bicine BCC pH 7.4 (1 M) 87.6 μl of sodium bicine BCC pH 9.4 (1 M) 400.0 μl of water (100 v/v)
B5	Polyethylene glycol 8,000 14.4 w/v - Glycerol 20 v/v - MES 0.08 M pH 6.5 Calcium acetate 0.16 M - 🔍	pH 6.5	160.0 μl of calcium acetate BCC (1 M) 250.0 μl of glycerol BCC (80 v/v) 720.0 μl of polyethylene glycol 8000 BCC (20 w/v) 22.2 μl of sodium MES BCC pH 5.2 (1 M) 57.8 μl of sodium MES BCC pH 7.2 (1 M)
B6	Polyethylene glycol 8,000 10 w/v - Imidazole 0.1 M pH 8 🔍	pH 8	100.0 μl of imidazole BCC pH 8 (1 M) 500.0 μl of polyethylene glycol 8000 BCC (20 w/v) 400.0 μl of water (100 v/v)
C1	Jeffamine® M-600 30 v/v - MES 0.1 M pH 6.5 Cesium chloride 0.05 M - 🔍	pH 6.5	50.0 μl of Cesium chloride (1 M) 600.0 μl of Jeffamine® M-600 (50 v/v) 33.3 μl of sodium MES BCC pH 6.5 (3 M) 316.7 μl of water (100 v/v)
C2	tri-Sodium citrate 0.1 M pH 5 Ammonium sulfate 3.15 M - 🔍	pH 5	787.5 μl of ammonium sulfate BCC (4 M) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 112.5 μl of water (100 v/v)
C3	2-methyl-2,4-pentanediol BCC 20 v/v - tris chloride BCC 0.1 M pH 8 🔍	pH 8	200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of tris chloride BCC pH 8 (1 M) 700.0 μl of water (100 v/v)
C4	Jeffamine® M-600 20 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	400.0 μl of Jeffamine® M-600 (50 v/v) 100.0 μl of sodium HEPES BCC pH 7.5 (1 M) 500.0 μl of water (100 v/v)
C5	Ethylene glycol 50 v/v - tris chloride BCC 0.1 M pH 8.5 Magnesium chloride 0.2 M - 🔍	pH 8.5	500.0 μl of Ethylene glycol (100 v/v) 80.0 μl of Magnesium chloride (2.5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 320.0 μl of water (100 v/v)
C6	2-methyl-2,4-pentanediol BCC 10 v/v - BICINE 0.1 M pH 9 🔍	pH 9	100.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of sodium bicine BCC pH 9 (1 M) 800.0 μl of water (100 v/v)
D1	di-Sodium succinate 0.8 M - 🔍	NA	266.7 μl of di-Sodium succinate pH 7 (3 M) 733.3 μl of water (100 v/v)
D2	di-Sodium DL-malate 2.1 M - 🔍	NA	700.0 μl of di-Sodium DL-malate pH 7 (3 M) 300.0 μl of water (100 v/v)
D3	di-Sodium malonate 2.4 M - 🔍	NA	800.0 μl of di-Sodium malonate pH 7 (3 M) 200.0 μl of water (100 v/v)
D4	di-Sodium malonate 1.1 M - Jeffamine® ED-2001 0.5 w/v - HEPES 0.1 M pH 7 🔍	pH 7	366.7 μl of di-Sodium malonate pH 7 (3 M) 10.0 μl of Jeffamine® ED-2001 pH 7 (50 w/v) 100.0 μl of sodium HEPES BCC pH 7 (1 M) 523.3 μl of water (100 v/v)
D5	Polyethylene glycol monomethyl ether 2,000 1 w/v - HEPES 0.1 M pH 7 di-Sodium succinate 1 M - 🔍	pH 7	333.3 μl of di-Sodium succinate pH 7 (3 M) 20.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100.0 μl of sodium HEPES BCC pH 7 (1 M) 546.7 μl of water (100 v/v)
D6	Jeffamine® M-600 30 v/v - HEPES 0.1 M pH 7 🔍	pH 7	600.0 μl of Jeffamine® M-600 pH 7 (50 v/v) 100.0 μl of sodium HEPES BCC pH 7 (1 M) 300.0 μl of water (100 v/v)