C6 - Screen Contents

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Supplemental Information

Screen Description

JCSG+ 4

JCSG / JCSG+ 4

Created 2009-11-16.

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JCSG+ 4

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JCSG+ 4

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Screen Structure

JCSG+ 4

Well Count	24
Number of distinct ingredients	27
Internal diversity (score mean dist)	0.868

Screen Composition

More information about screen composition chart.

ingredient type	JCSG+ 4
Buffer	27
Organic (non-volatile)	3
Polymer	18
Salt	24

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

JCSG+ 4 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Jeffamine® ED-2001 30 w/v - HEPES 0.1 M pH 7 🔍	pH 7	100.0 μl of HEPES pH 7 (1 M) 600.0 μl of Jeffamine® ED-2001 pH 7 (50 w/v) 300.0 μl of water (100 v/v)
A2	Poly(acrylic acid sodium salt) 5,100 22 w/v - HEPES 0.1 M pH 7.5 Magnesium chloride 0.02 M - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 8.0 μl of Magnesium chloride (2.5 M) 440.0 μl of Poly(acrylic acid sodium salt) 5,100 (50 w/v) 452.0 μl of water (100 v/v)
A3	Polyvinylpyrrolidone K15 20 w/v - tris chloride BCC 0.1 M pH 8.5 Cobalt (II) chloride 0.1 M - 🔍	pH 8.5	100.0 μl of Cobalt (II) chloride (1 M) 400.0 μl of Polyvinylpyrrolidone K15 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 400.0 μl of water (100 v/v)
A4	Polyethylene glycol 2,000 20 w/v - tris chloride BCC 0.1 M pH 8.5 Trimethylamine N-oxide 0.2 M - 🔍	pH 8.5	400.0 μl of Polyethylene glycol 2,000 (50 w/v) 200.0 μl of Trimethylamine N-oxide (1 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 300.0 μl of water (100 v/v)
A5	Polyethylene glycol 3,350 12 w/v - Cobalt (II) chloride 0.005 M - Cadmium chloride 0.005 M - Nickel (II) chloride 0.005 M - Magnesium chloride 0.005 M - HEPES 0.1 M pH 7.5 🔍	pH 7.5	5.0 μl of Cadmium chloride (1 M) 5.0 μl of Cobalt (II) chloride (1 M) 100.0 μl of HEPES pH 7.5 (1 M) 5.0 μl of Magnesium chloride pH 7 (1 M) 5.0 μl of Nickel (II) chloride pH 7 (1 M) 240.0 μl of Polyethylene glycol 3,350 (50 w/v) 640.0 μl of water (100 v/v)
A6	Polyethylene glycol 3,350 20 w/v - di-Sodium malonate 0.2 M - 🔍	NA	400.0 μl of Polyethylene glycol 3,350 (50 w/v) 66.7 μl of di-Sodium malonate pH 7 (3 M) 533.3 μl of water (100 v/v)
B1	Polyethylene glycol 3,350 15 w/v - di-Sodium succinate 0.1 M - 🔍	NA	300.0 μl of Polyethylene glycol 3,350 (50 w/v) 33.3 μl of di-Sodium succinate pH 7 (3 M) 666.7 μl of water (100 v/v)
B2	Polyethylene glycol 3,350 20 w/v - di-Sodium DL-malate 0.15 M - 🔍	NA	400.0 μl of Polyethylene glycol 3,350 (50 w/v) 50.0 μl of di-Sodium DL-malate pH 7 (3 M) 550.0 μl of water (100 v/v)
B3	Polyethylene glycol monomethyl ether 2,000 30 w/v - Potassium thiocyanate 0.1 M - 🔍	NA	600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 12.5 μl of Potassium thiocyanate (8 M) 387.5 μl of water (100 v/v)
B4	Polyethylene glycol monomethyl ether 2,000 30 w/v - Potassium bromide 0.15 M - 🔍	NA	600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 37.5 μl of Potassium bromide (4 M) 362.5 μl of water (100 v/v)
B5	BIS-TRIS 0.1 M pH 5.5 Ammonium sulfate 2 M - 🔍	pH 5.5	666.7 μl of Ammonium sulfate (3 M) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 233.3 μl of water (100 v/v)
B6	BIS-TRIS 0.1 M pH 5.5 Sodium chloride 3 M - 🔍	pH 5.5	100.0 μl of BIS-TRIS pH 5.5 (1 M) 600.0 μl of Sodium chloride (5 M) 300.0 μl of water (100 v/v)
C1	BIS-TRIS 0.1 M pH 5.5 Magnesium formate 0.3 M - 🔍	pH 5.5	100.0 μl of BIS-TRIS pH 5.5 (1 M) 300.0 μl of Magnesium formate (1 M) 600.0 μl of water (100 v/v)
C2	BIS-TRIS 0.1 M pH 5.5 Polyethylene glycol 3,350 1 w/v - Ammonium sulfate 1 M - 🔍	pH 5.5	333.3 μl of Ammonium sulfate (3 M) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 20.0 μl of Polyethylene glycol 3,350 (50 w/v) 546.7 μl of water (100 v/v)
C3	BIS-TRIS 0.1 M pH 5.5 Polyethylene glycol 3,350 25 w/v - 🔍	pH 5.5	100.0 μl of BIS-TRIS pH 5.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 400.0 μl of water (100 v/v)
C4	BIS-TRIS 0.1 M pH 5.5 2-methyl-2,4-pentanediol BCC 45 v/v - Calcium chloride 0.2 M - 🔍	pH 5.5	450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 200.0 μl of Calcium chloride (1 M) 250.0 μl of water (100 v/v)
C5	BIS-TRIS 0.1 M pH 5.5 2-methyl-2,4-pentanediol BCC 45 v/v - Ammonium acetate 0.2 M - 🔍	pH 5.5	450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 200.0 μl of Ammonium acetate (1 M) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 250.0 μl of water (100 v/v)
C6	BIS-TRIS 0.1 M pH 5.5 Ammonium acetate 0.1 M - Polyethylene glycol 10,000 17 w/v - 🔍	pH 5.5	100.0 μl of Ammonium acetate (1 M) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 340.0 μl of Polyethylene glycol 10,000 (50 w/v) 460.0 μl of water (100 v/v)
D1	BIS-TRIS 0.1 M pH 5.5 Polyethylene glycol 3,350 25 w/v - Ammonium sulfate 0.2 M - 🔍	pH 5.5	66.7 μl of Ammonium sulfate (3 M) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 333.3 μl of water (100 v/v)
D2	BIS-TRIS 0.1 M pH 5.5 Polyethylene glycol 3,350 25 w/v - Sodium chloride 0.2 M - 🔍	pH 5.5	100.0 μl of BIS-TRIS pH 5.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 40.0 μl of Sodium chloride (5 M) 360.0 μl of water (100 v/v)
D3	BIS-TRIS 0.1 M pH 5.5 Polyethylene glycol 3,350 25 w/v - Lithium sulfate 0.2 M - 🔍	pH 5.5	100.0 μl of BIS-TRIS pH 5.5 (1 M) 80.0 μl of Lithium sulfate (2.5 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 320.0 μl of water (100 v/v)
D4	BIS-TRIS 0.1 M pH 5.5 Polyethylene glycol 3,350 25 w/v - Ammonium acetate 0.2 M - 🔍	pH 5.5	200.0 μl of Ammonium acetate (1 M) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 200.0 μl of water (100 v/v)
D5	BIS-TRIS 0.1 M pH 5.5 Polyethylene glycol 3,350 25 w/v - Magnesium chloride 0.2 M - 🔍	pH 5.5	100.0 μl of BIS-TRIS pH 5.5 (1 M) 80.0 μl of Magnesium chloride (2.5 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 320.0 μl of water (100 v/v)
D6	2-methyl-2,4-pentanediol BCC 45 v/v - HEPES 0.1 M pH 7.5 Ammonium acetate 0.2 M - 🔍	pH 7.5	450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 200.0 μl of Ammonium acetate (1 M) 100.0 μl of HEPES pH 7.5 (1 M) 250.0 μl of water (100 v/v)