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Supplemental Information

Screen Description

JCSG+ 4

JCSG / JCSG+ 4
Created 2009-11-16.

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JCSG+ 4

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JCSG+ 4

    Screen Structure

    JCSG+ 4

    Well Count 24
    Number of distinct ingredients 27
    Internal diversity (score mean dist) 0.868

    Screen Composition

    More information about screen composition chart.
    2025-06-14T13:35:40.199994 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type JCSG+ 4
    Buffer 27
    Organic (non-volatile) 3
    Polymer 18
    Salt 24

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    JCSG+ 4 Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Jeffamine® ED-2001 30 w/v -
    • HEPES 0.1 M pH 7
    		pH 7
    600 μl of Jeffamine® ED-2001 pH 7 (50 w/v)
    100 μl of HEPES pH 7 (1 M)
    300 μl of water (100 v/v)
    A2
    • Poly(acrylic acid sodium salt) 5,100 22 w/v -
    • HEPES 0.1 M pH 7.5
    • Magnesium chloride 0.02 M -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    440 μl of Poly(acrylic acid sodium salt) 5,100 (50 w/v)
    8 μl of Magnesium chloride (2.5 M)
    452 μl of water (100 v/v)
    A3
    • Polyvinylpyrrolidone K15 20 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Cobalt (II) chloride 0.1 M -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Polyvinylpyrrolidone K15 (50 w/v)
    100 μl of Cobalt (II) chloride (1 M)
    400 μl of water (100 v/v)
    A4
    • Polyethylene glycol 2,000 20 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Trimethylamine N-oxide 0.2 M -
    		pH 8.5
    200 μl of Trimethylamine N-oxide (1 M)
    400 μl of Polyethylene glycol 2,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    300 μl of water (100 v/v)
    A5
    • Polyethylene glycol 3,350 12 w/v -
    • Cobalt (II) chloride 0.005 M -
    • Cadmium chloride 0.005 M -
    • Nickel (II) chloride 0.005 M -
    • Magnesium chloride 0.005 M -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    5 μl of Magnesium chloride pH 7 (1 M)
    5 μl of Nickel (II) chloride pH 7 (1 M)
    5 μl of Cadmium chloride (1 M)
    5 μl of Cobalt (II) chloride (1 M)
    640 μl of water (100 v/v)
    A6
    • Polyethylene glycol 3,350 20 w/v -
    • di-Sodium malonate 0.2 M -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    66 μl of di-Sodium malonate pH 7 (3 M)
    533 μl of water (100 v/v)
    B1
    • Polyethylene glycol 3,350 15 w/v -
    • di-Sodium succinate 0.1 M -
    		NA
    33 μl of di-Sodium succinate pH 7 (3 M)
    300 μl of Polyethylene glycol 3,350 (50 w/v)
    666 μl of water (100 v/v)
    B2
    • Polyethylene glycol 3,350 20 w/v -
    • di-Sodium DL-malate 0.15 M -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    50 μl of di-Sodium DL-malate pH 7 (3 M)
    550 μl of water (100 v/v)
    B3
    • Polyethylene glycol monomethyl ether 2,000 30 w/v -
    • Potassium thiocyanate 0.1 M -
    		NA
    12 μl of Potassium thiocyanate (8 M)
    600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    387 μl of water (100 v/v)
    B4
    • Polyethylene glycol monomethyl ether 2,000 30 w/v -
    • Potassium bromide 0.15 M -
    		NA
    600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    37 μl of Potassium bromide (4 M)
    362 μl of water (100 v/v)
    B5
    • BIS-TRIS 0.1 M pH 5.5
    • Ammonium sulfate 2 M -
    		pH 5.5
    100 μl of BIS-TRIS pH 5.5 (1 M)
    666 μl of Ammonium sulfate (3 M)
    233 μl of water (100 v/v)
    B6
    • BIS-TRIS 0.1 M pH 5.5
    • Sodium chloride 3 M -
    		pH 5.5
    100 μl of BIS-TRIS pH 5.5 (1 M)
    600 μl of Sodium chloride (5 M)
    300 μl of water (100 v/v)
    C1
    • BIS-TRIS 0.1 M pH 5.5
    • Magnesium formate 0.3 M -
    		pH 5.5
    300 μl of Magnesium formate (1 M)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    600 μl of water (100 v/v)
    C2
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 3,350 1 w/v -
    • Ammonium sulfate 1 M -
    		pH 5.5
    20 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    333 μl of Ammonium sulfate (3 M)
    546 μl of water (100 v/v)
    C3
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 3,350 25 w/v -
    		pH 5.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    400 μl of water (100 v/v)
    C4
    • BIS-TRIS 0.1 M pH 5.5
    • 2-methyl-2,4-pentanediol BCC 45 v/v -
    • Calcium chloride 0.2 M -
    		pH 5.5
    100 μl of BIS-TRIS pH 5.5 (1 M)
    450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    200 μl of Calcium chloride (1 M)
    250 μl of water (100 v/v)
    C5
    • BIS-TRIS 0.1 M pH 5.5
    • 2-methyl-2,4-pentanediol BCC 45 v/v -
    • Ammonium acetate 0.2 M -
    		pH 5.5
    200 μl of Ammonium acetate (1 M)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    250 μl of water (100 v/v)
    C6
    • BIS-TRIS 0.1 M pH 5.5
    • Ammonium acetate 0.1 M -
    • Polyethylene glycol 10,000 17 w/v -
    		pH 5.5
    100 μl of Ammonium acetate (1 M)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    340 μl of Polyethylene glycol 10,000 (50 w/v)
    460 μl of water (100 v/v)
    D1
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 3,350 25 w/v -
    • Ammonium sulfate 0.2 M -
    		pH 5.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    66 μl of Ammonium sulfate (3 M)
    333 μl of water (100 v/v)
    D2
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 3,350 25 w/v -
    • Sodium chloride 0.2 M -
    		pH 5.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    40 μl of Sodium chloride (5 M)
    360 μl of water (100 v/v)
    D3
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 3,350 25 w/v -
    • Lithium sulfate 0.2 M -
    		pH 5.5
    80 μl of Lithium sulfate (2.5 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    320 μl of water (100 v/v)
    D4
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 3,350 25 w/v -
    • Ammonium acetate 0.2 M -
    		pH 5.5
    200 μl of Ammonium acetate (1 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    200 μl of water (100 v/v)
    D5
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 3,350 25 w/v -
    • Magnesium chloride 0.2 M -
    		pH 5.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    80 μl of Magnesium chloride (2.5 M)
    320 μl of water (100 v/v)
    D6
    • 2-methyl-2,4-pentanediol BCC 45 v/v -
    • HEPES 0.1 M pH 7.5
    • Ammonium acetate 0.2 M -
    		pH 7.5
    200 μl of Ammonium acetate (1 M)
    100 μl of HEPES pH 7.5 (1 M)
    450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    250 μl of water (100 v/v)