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Supplemental Information

Screen Description

JCSG+ HTS

JCSG / JCSG+ HTS
Created 2009-11-16.

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JCSG+ HTS

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JCSG+ HTS

    Screen Structure

    JCSG+ HTS

    Well Count 96
    Number of distinct ingredients 71
    Internal diversity (score mean dist) 0.961

    Screen Composition

    More information about screen composition chart.
    2025-06-14T13:39:21.876465 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type JCSG+ HTS
    Buffer 101
    Cryoprotectant 6
    Organic (non-volatile) 25
    Organic (volatile) 6
    Polymer 65
    Salt 90

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    JCSG+ HTS Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Polyethylene glycol 400 50 v/v -
    • Sodium acetate 0.1 M pH 4.5
    • Lithium sulfate 0.2 M -
    		pH 4.5
    80 μl of Lithium sulfate (2.5 M)
    50 μl of Sodium acetate pH 4.5 (2 M)
    500 μl of Polyethylene glycol 400 (100 v/v)
    370 μl of water (100 v/v)
    A2
    • Polyethylene glycol 3,000 20 w/v -
    • tri-Sodium citrate 0.1 M pH 5.5
    		pH 5.5
    400 μl of Polyethylene glycol 3,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    500 μl of water (100 v/v)
    A3
    • Polyethylene glycol 3,350 20 w/v -
    • di-Ammonium hydrogen citrate 0.2 M -
    		NA
    80 μl of di-Ammonium hydrogen citrate pH 5 (2.5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    520 μl of water (100 v/v)
    A4
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    • Sodium acetate 0.1 M pH 4.6
    • Calcium chloride 0.02 M -
    		pH 4.6
    4 μl of Calcium chloride (5 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    596 μl of water (100 v/v)
    A5
    • Polyethylene glycol 3,350 20 w/v -
    • Magnesium formate 0.2 M -
    		NA
    200 μl of Magnesium formate pH 5.9 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    400 μl of water (100 v/v)
    A6
    • Potassium phosphate citrate 0.1 M pH 4.2
    • Polyethylene glycol 1,000 20 w/v -
    • Lithium sulfate 0.2 M -
    		pH 4.2
    80 μl of Lithium sulfate (2.5 M)
    400 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of Potassium phosphate citrate pH 4.2 (1 M)
    420 μl of water (100 v/v)
    A7
    • Polyethylene glycol 8,000 20 w/v -
    • CHES 0.1 M pH 9.5
    		pH 9.5
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of CHES pH 9.5 (1 M)
    500 μl of water (100 v/v)
    A8
    • Polyethylene glycol 3,350 20 w/v -
    • Ammonium formate 0.2 M -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    40 μl of Ammonium formate pH 6.6 (5 M)
    560 μl of water (100 v/v)
    A9
    • Polyethylene glycol 3,350 20 w/v -
    • Ammonium chloride 0.2 M -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    40 μl of Ammonium chloride pH 6.3 (5 M)
    560 μl of water (100 v/v)
    A10
    • Polyethylene glycol 3,350 20 w/v -
    • Potassium formate 0.2 M -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    14 μl of Potassium formate pH 7.3 (14 M)
    585 μl of water (100 v/v)
    A11
    • 2-methyl-2,4-pentanediol BCC 50 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium di-hydrogen phosphate 0.2 M -
    		pH 8.5
    500 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    200 μl of Ammonium di-hydrogen phosphate (1 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    200 μl of water (100 v/v)
    A12
    • Polyethylene glycol 3,350 20 w/v -
    • Potassium nitrate 0.2 M -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    66 μl of Potassium nitrate pH 6.9 (3 M)
    533 μl of water (100 v/v)
    B1
    • tri-Sodium citrate 0.1 M pH 4
    • Ammonium sulfate 0.8 M -
    		pH 4
    100 μl of tri-Sodium citrate pH 4 (1 M)
    266 μl of Ammonium sulfate (3 M)
    633 μl of water (100 v/v)
    B2
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium thiocyanate 0.2 M -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    25 μl of Sodium thiocyanate pH 6.9 (8 M)
    575 μl of water (100 v/v)
    B3
    • Polyethylene glycol 6,000 20 w/v -
    • BICINE 0.1 M pH 9
    		pH 9
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of BICINE pH 9 (1 M)
    500 μl of water (100 v/v)
    B4
    • Polyethylene glycol 8,000 10 w/v -
    • Ethylene glycol 8 v/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    80 μl of Ethylene glycol (100 v/v)
    620 μl of water (100 v/v)
    B5
    • 2-methyl-2,4-pentanediol BCC 40 v/v -
    • Polyethylene glycol 8,000 5 w/v -
    • MES 0.1 M pH 6.5
    		pH 6.5
    400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of MES pH 6.5 (1 M)
    400 μl of water (100 v/v)
    B6
    • Ethanol 40 v/v -
    • Polyethylene glycol 1,000 5 w/v -
    • Potassium phosphate citrate 0.1 M pH 4.2
    		pH 4.2
    100 μl of Polyethylene glycol 1,000 (50 w/v)
    800 μl of Ethanol (50 v/v)
    100 μl of Potassium phosphate citrate pH 4.2 (1 M)
    B7
    • Polyethylene glycol 4,000 8 w/v -
    • Sodium acetate 0.1 M pH 4.6
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    160 μl of Polyethylene glycol 4,000 (50 w/v)
    740 μl of water (100 v/v)
    B8
    • Polyethylene glycol 8,000 10 w/v -
    • tris chloride BCC 0.1 M pH 7
    • Magnesium chloride 0.2 M -
    		pH 7
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    80 μl of Magnesium chloride (2.5 M)
    33 μl of tris chloride BCC pH 7 (3 M)
    686 μl of water (100 v/v)
    B9
    • Polyethylene glycol 6,000 20 w/v -
    • tri-Sodium citrate 0.1 M pH 5
    		pH 5
    100 μl of tri-Sodium citrate pH 5 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    500 μl of water (100 v/v)
    B10
    • Polyethylene glycol 200 50 v/v -
    • MES 0.1 M pH 6.5
    • Magnesium chloride 0.2 M -
    		pH 6.5
    500 μl of Polyethylene glycol 200 (100 v/v)
    100 μl of MES pH 6.5 (1 M)
    80 μl of Magnesium chloride (2.5 M)
    320 μl of water (100 v/v)
    B11
    • tri-Sodium citrate 1.6 M -
    		NA
    800 μl of tri-Sodium citrate (2 M)
    200 μl of water (100 v/v)
    B12
    • Polyethylene glycol 3,350 20 w/v -
    • tri-Potassium citrate 0.2 M -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    80 μl of tri-Potassium citrate pH 8.3 (2.5 M)
    520 μl of water (100 v/v)
    C1
    • Polyethylene glycol 8,000 20 w/v -
    • Potassium phosphate citrate 0.1 M pH 4.2
    • Sodium chloride 0.2 M -
    		pH 4.2
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Sodium chloride (1 M)
    100 μl of Potassium phosphate citrate pH 4.2 (1 M)
    300 μl of water (100 v/v)
    C2
    • Polyethylene glycol 6,000 20 w/v -
    • tri-Sodium citrate 0.1 M pH 4
    • Lithium chloride 1 M -
    		pH 4
    100 μl of tri-Sodium citrate pH 4 (1 M)
    200 μl of Lithium chloride (5 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    300 μl of water (100 v/v)
    C3
    • Polyethylene glycol 3,350 20 w/v -
    • Ammonium nitrate 0.2 M -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    20 μl of Ammonium nitrate pH 6.3 (10 M)
    580 μl of water (100 v/v)
    C4
    • Polyethylene glycol 6,000 10 w/v -
    • HEPES 0.1 M pH 7
    		pH 7
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of HEPES pH 7 (1 M)
    700 μl of water (100 v/v)
    C5
    • Sodium di-hydrogen phosphate 0.8 M -
    • Potassium di-hydrogen phosphate 0.8 M -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    400 μl of Potassium di-hydrogen phosphate (2 M)
    200 μl of Sodium di-hydrogen phosphate (4 M)
    100 μl of HEPES pH 7.5 (1 M)
    300 μl of water (100 v/v)
    C6
    • Polyethylene glycol 300 40 v/v -
    • Potassium phosphate citrate 0.1 M pH 4.2
    		pH 4.2
    400 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of Potassium phosphate citrate pH 4.2 (1 M)
    500 μl of water (100 v/v)
    C7
    • Polyethylene glycol 3,000 10 w/v -
    • Sodium acetate 0.1 M pH 4.5
    • Zinc acetate 0.2 M -
    		pH 4.5
    200 μl of Polyethylene glycol 3,000 (50 w/v)
    80 μl of Zinc acetate (2.5 M)
    50 μl of Sodium acetate pH 4.5 (2 M)
    670 μl of water (100 v/v)
    C8
    • Ethanol 20 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    400 μl of Ethanol (50 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    500 μl of water (100 v/v)
    C9
    • 1,2-Propanediol 25 v/v -
    • Glycerol 10 v/v -
    • Sodium Potassium phosphate 0.1 M pH 6.2
    		pH 6.2
    100 μl of Glycerol (100 v/v)
    250 μl of 1,2-Propanediol (100 v/v)
    25 μl of Sodium Potassium phosphate pH 6.2 (4 M)
    625 μl of water (100 v/v)
    C10
    • Polyethylene glycol 20,000 10 w/v -
    • BICINE 0.1 M pH 9
    • 1,4-Dioxane 2 v/v -
    		pH 9
    200 μl of Polyethylene glycol 20,000 (50 w/v)
    40 μl of 1,4-Dioxane (50 v/v)
    100 μl of BICINE pH 9 (1 M)
    660 μl of water (100 v/v)
    C11
    • Sodium acetate 0.1 M pH 4.6
    • Ammonium sulfate 2 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    666 μl of Ammonium sulfate (3 M)
    233 μl of water (100 v/v)
    C12
    • Polyethylene glycol 1,000 10 w/v -
    • Polyethylene glycol 8,000 10 w/v -
    		NA
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Polyethylene glycol 1,000 (50 w/v)
    600 μl of water (100 v/v)
    D1
    • Polyethylene glycol 1,500 24 w/v -
    • Glycerol 20 v/v -
    		NA
    200 μl of Glycerol (100 v/v)
    480 μl of Polyethylene glycol 1,500 (50 w/v)
    320 μl of water (100 v/v)
    D2
    • Polyethylene glycol 400 30 v/v -
    • HEPES 0.1 M pH 7.5
    • Magnesium chloride 0.2 M -
    		pH 7.5
    80 μl of Magnesium chloride (2.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    520 μl of water (100 v/v)
    D3
    • Polyethylene glycol 200 50 v/v -
    • Sodium Potassium phosphate 0.1 M pH 6.2
    • Sodium chloride 0.2 M -
    		pH 6.2
    500 μl of Polyethylene glycol 200 (100 v/v)
    40 μl of Sodium chloride (5 M)
    25 μl of Sodium Potassium phosphate pH 6.2 (4 M)
    435 μl of water (100 v/v)
    D4
    • Polyethylene glycol 8,000 30 w/v -
    • Sodium acetate 0.1 M pH 4.5
    • Lithium sulfate 0.2 M -
    		pH 4.5
    600 μl of Polyethylene glycol 8,000 (50 w/v)
    80 μl of Lithium sulfate (2.5 M)
    50 μl of Sodium acetate pH 4.5 (2 M)
    270 μl of water (100 v/v)
    D5
    • 2-methyl-2,4-pentanediol BCC 70 v/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    700 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    200 μl of water (100 v/v)
    D6
    • Polyethylene glycol 8,000 20 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Magnesium chloride 0.2 M -
    		pH 8.5
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    80 μl of Magnesium chloride (2.5 M)
    420 μl of water (100 v/v)
    D7
    • Polyethylene glycol 400 40 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Lithium sulfate 0.2 M -
    		pH 8.5
    80 μl of Lithium sulfate (2.5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Polyethylene glycol 400 (100 v/v)
    420 μl of water (100 v/v)
    D8
    • 2-methyl-2,4-pentanediol BCC 40 v/v -
    • tris chloride BCC 0.1 M pH 8
    		pH 8
    400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    500 μl of water (100 v/v)
    D9
    • Polyethylene glycol 4,000 25.5 w/v -
    • Glycerol 15 v/v -
    • Ammonium sulfate 0.17 M -
    		NA
    150 μl of Glycerol (100 v/v)
    56 μl of Ammonium sulfate (3 M)
    510 μl of Polyethylene glycol 4,000 (50 w/v)
    283 μl of water (100 v/v)
    D10
    • Polyethylene glycol 300 40 v/v -
    • MES 0.1 M pH 6.5
    • Calcium acetate 0.2 M -
    		pH 6.5
    400 μl of Polyethylene glycol 300 (100 v/v)
    33 μl of MES pH 6.5 (3 M)
    100 μl of Calcium acetate (2 M)
    466 μl of water (100 v/v)
    D11
    • 2-Propanol 14 v/v -
    • Glycerol 30 v/v -
    • Sodium acetate 0.07 M pH 4.6
    • Calcium chloride 0.14 M -
    		pH 4.6
    140 μl of 2-Propanol (100 v/v)
    28 μl of Calcium chloride (5 M)
    300 μl of Glycerol (100 v/v)
    70 μl of Sodium acetate pH 4.6 (1 M)
    462 μl of water (100 v/v)
    D12
    • Polyethylene glycol 8,000 16 w/v -
    • Glycerol 20 v/v -
    • Potassium di-hydrogen phosphate 0.04 M -
    		NA
    200 μl of Glycerol (100 v/v)
    320 μl of Polyethylene glycol 8,000 (50 w/v)
    20 μl of Potassium di-hydrogen phosphate (2 M)
    460 μl of water (100 v/v)
    E1
    • MES 0.1 M pH 6.5
    • tri-Sodium citrate 1 M -
    		pH 6.5
    33 μl of MES pH 6.5 (3 M)
    500 μl of tri-Sodium citrate (2 M)
    466 μl of water (100 v/v)
    E2
    • Ammonium sulfate 2 M -
    • MES 0.1 M pH 6.5
    • Sodium chloride 0.2 M -
    		pH 6.5
    33 μl of MES pH 6.5 (3 M)
    40 μl of Sodium chloride (5 M)
    400 μl of Ammonium sulfate (5 M)
    526 μl of water (100 v/v)
    E3
    • 2-Propanol 10 v/v -
    • HEPES 0.1 M pH 7.5
    • Sodium chloride 0.2 M -
    		pH 7.5
    100 μl of 2-Propanol (100 v/v)
    40 μl of Sodium chloride (5 M)
    100 μl of HEPES pH 7.5 (1 M)
    760 μl of water (100 v/v)
    E4
    • Ammonium sulfate 1.26 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Lithium sulfate 0.2 M -
    		pH 8.5
    80 μl of Lithium sulfate (2.5 M)
    420 μl of Ammonium sulfate (3 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of water (100 v/v)
    E5
    • 2-methyl-2,4-pentanediol BCC 40 v/v -
    • CAPS 0.1 M pH 10
    		pH 10
    100 μl of CAPS pH 10 (1 M)
    400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    500 μl of water (100 v/v)
    E6
    • Polyethylene glycol 3,000 20 w/v -
    • Imidazole 0.1 M pH 8
    • Zinc acetate 0.2 M -
    		pH 8
    400 μl of Polyethylene glycol 3,000 (50 w/v)
    100 μl of Imidazole pH 8 (1 M)
    100 μl of Zinc acetate (2 M)
    400 μl of water (100 v/v)
    E7
    • 2-Propanol 10 v/v -
    • MES 0.1 M pH 6.5
    • Zinc acetate 0.2 M -
    		pH 6.5
    100 μl of 2-Propanol (100 v/v)
    33 μl of MES pH 6.5 (3 M)
    100 μl of Zinc acetate (2 M)
    766 μl of water (100 v/v)
    E8
    • Sodium acetate 0.1 M pH 4.5
    • di-Ammonium hydrogen phosphate 1 M -
    		pH 4.5
    285 μl of di-Ammonium hydrogen phosphate (3.5 M)
    33 μl of Sodium acetate pH 4.5 (3 M)
    681 μl of water (100 v/v)
    E9
    • MES 0.1 M pH 6.5
    • Magnesium sulfate 1.6 M -
    		pH 6.5
    800 μl of Magnesium sulfate (2 M)
    33 μl of MES pH 6.5 (3 M)
    166 μl of water (100 v/v)
    E10
    • Polyethylene glycol 6,000 10 w/v -
    • BICINE 0.1 M pH 9
    		pH 9
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of BICINE pH 9 (1 M)
    700 μl of water (100 v/v)
    E11
    • Polyethylene glycol 8,000 14.4 w/v -
    • Glycerol 20 v/v -
    • MES 0.08 M pH 6.5
    • Calcium acetate 0.16 M -
    		pH 6.5
    160 μl of Calcium acetate (1 M)
    200 μl of Glycerol (100 v/v)
    288 μl of Polyethylene glycol 8,000 (50 w/v)
    26 μl of MES pH 6.5 (3 M)
    325 μl of water (100 v/v)
    E12
    • Polyethylene glycol 8,000 10 w/v -
    • Imidazole 0.1 M pH 8
    		pH 8
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Imidazole pH 8 (1 M)
    700 μl of water (100 v/v)
    F1
    • Jeffamine® M-600 30 v/v -
    • MES 0.1 M pH 6.5
    • Cesium chloride 0.05 M -
    		pH 6.5
    50 μl of Cesium chloride (1 M)
    600 μl of Jeffamine® M-600 (50 v/v)
    33 μl of MES pH 6.5 (3 M)
    316 μl of water (100 v/v)
    F2
    • tri-Sodium citrate 0.1 M pH 5
    • Ammonium sulfate 3.15 M -
    		pH 5
    100 μl of tri-Sodium citrate pH 5 (1 M)
    787 μl of Ammonium sulfate (4 M)
    112 μl of water (100 v/v)
    F3
    • 2-methyl-2,4-pentanediol BCC 20 v/v -
    • tris chloride BCC 0.1 M pH 8
    		pH 8
    200 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    700 μl of water (100 v/v)
    F4
    • Jeffamine® M-600 20 v/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    400 μl of Jeffamine® M-600 (50 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of water (100 v/v)
    F5
    • Ethylene glycol 50 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Magnesium chloride 0.2 M -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    80 μl of Magnesium chloride (2.5 M)
    500 μl of Ethylene glycol (100 v/v)
    320 μl of water (100 v/v)
    F6
    • 2-methyl-2,4-pentanediol BCC 10 v/v -
    • BICINE 0.1 M pH 9
    		pH 9
    100 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of BICINE pH 9 (1 M)
    800 μl of water (100 v/v)
    F7
    • di-Sodium succinate 0.8 M -
    		NA
    266 μl of di-Sodium succinate pH 7 (3 M)
    733 μl of water (100 v/v)
    F8
    • di-Sodium DL-malate 2.1 M -
    		NA
    700 μl of di-Sodium DL-malate pH 7 (3 M)
    300 μl of water (100 v/v)
    F9
    • di-Sodium malonate 2.4 M -
    		NA
    800 μl of di-Sodium malonate pH 7 (3 M)
    200 μl of water (100 v/v)
    F10
    • di-Sodium malonate 1.1 M -
    • Jeffamine® ED-2001 0.5 w/v -
    • HEPES 0.1 M pH 7
    		pH 7
    10 μl of Jeffamine® ED-2001 pH 7 (50 w/v)
    366 μl of di-Sodium malonate pH 7 (3 M)
    100 μl of HEPES pH 7 (1 M)
    523 μl of water (100 v/v)
    F11
    • Polyethylene glycol monomethyl ether 2,000 1 w/v -
    • HEPES 0.1 M pH 7
    • di-Sodium succinate 1 M -
    		pH 7
    333 μl of di-Sodium succinate pH 7 (3 M)
    20 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of HEPES pH 7 (1 M)
    546 μl of water (100 v/v)
    F12
    • Jeffamine® M-600 30 v/v -
    • HEPES 0.1 M pH 7
    		pH 7
    600 μl of Jeffamine® M-600 pH 7 (50 v/v)
    100 μl of HEPES pH 7 (1 M)
    300 μl of water (100 v/v)
    G1
    • Jeffamine® ED-2001 30 w/v -
    • HEPES 0.1 M pH 7
    		pH 7
    600 μl of Jeffamine® ED-2001 pH 7 (50 w/v)
    100 μl of HEPES pH 7 (1 M)
    300 μl of water (100 v/v)
    G2
    • Poly(acrylic acid sodium salt) 5,100 22 w/v -
    • HEPES 0.1 M pH 7.5
    • Magnesium chloride 0.02 M -
    		pH 7.5
    440 μl of Poly(acrylic acid sodium salt) 5,100 (50 w/v)
    8 μl of Magnesium chloride (2.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    452 μl of water (100 v/v)
    G3
    • Polyvinylpyrrolidone K15 20 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Cobalt (II) chloride 0.1 M -
    		pH 8.5
    400 μl of Polyvinylpyrrolidone K15 (50 w/v)
    100 μl of Cobalt (II) chloride (1 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of water (100 v/v)
    G4
    • Polyethylene glycol 2,000 20 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Trimethylamine N-oxide 0.2 M -
    		pH 8.5
    400 μl of Polyethylene glycol 2,000 (50 w/v)
    200 μl of Trimethylamine N-oxide (1 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    300 μl of water (100 v/v)
    G5
    • Polyethylene glycol 3,350 12 w/v -
    • Cobalt (II) chloride 0.005 M -
    • Cadmium chloride 0.005 M -
    • Nickel (II) chloride 0.005 M -
    • Magnesium chloride 0.005 M -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    5 μl of Cadmium chloride (1 M)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    5 μl of Nickel (II) chloride (1 M)
    5 μl of Cobalt (II) chloride (1 M)
    2 μl of Magnesium chloride (2.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    643 μl of water (100 v/v)
    G6
    • Polyethylene glycol 3,350 20 w/v -
    • di-Sodium malonate 0.2 M -
    		NA
    66 μl of di-Sodium malonate pH 7 (3 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    533 μl of water (100 v/v)
    G7
    • Polyethylene glycol 3,350 15 w/v -
    • di-Sodium succinate 0.1 M -
    		NA
    33 μl of di-Sodium succinate pH 7 (3 M)
    300 μl of Polyethylene glycol 3,350 (50 w/v)
    666 μl of water (100 v/v)
    G8
    • Polyethylene glycol 3,350 20 w/v -
    • di-Sodium DL-malate 0.15 M -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    50 μl of di-Sodium DL-malate pH 7 (3 M)
    550 μl of water (100 v/v)
    G9
    • Polyethylene glycol monomethyl ether 2,000 30 w/v -
    • Potassium thiocyanate 0.1 M -
    		NA
    600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    12 μl of Potassium thiocyanate (8 M)
    387 μl of water (100 v/v)
    G10
    • Polyethylene glycol monomethyl ether 2,000 30 w/v -
    • Potassium bromide 0.15 M -
    		NA
    600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    37 μl of Potassium bromide (4 M)
    362 μl of water (100 v/v)
    G11
    • BIS-TRIS 0.1 M pH 5.5
    • Ammonium sulfate 2 M -
    		pH 5.5
    100 μl of BIS-TRIS pH 5.5 (1 M)
    666 μl of Ammonium sulfate (3 M)
    233 μl of water (100 v/v)
    G12
    • BIS-TRIS 0.1 M pH 5.5
    • Sodium chloride 3 M -
    		pH 5.5
    600 μl of Sodium chloride (5 M)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    300 μl of water (100 v/v)
    H1
    • BIS-TRIS 0.1 M pH 5.5
    • Magnesium formate 0.3 M -
    		pH 5.5
    300 μl of Magnesium formate (1 M)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    600 μl of water (100 v/v)
    H2
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 3,350 1 w/v -
    • Ammonium sulfate 1 M -
    		pH 5.5
    20 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    333 μl of Ammonium sulfate (3 M)
    546 μl of water (100 v/v)
    H3
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 3,350 25 w/v -
    		pH 5.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    400 μl of water (100 v/v)
    H4
    • BIS-TRIS 0.1 M pH 5.5
    • 2-methyl-2,4-pentanediol BCC 45 v/v -
    • Calcium chloride 0.2 M -
    		pH 5.5
    200 μl of Calcium chloride (1 M)
    450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    250 μl of water (100 v/v)
    H5
    • BIS-TRIS 0.1 M pH 5.5
    • 2-methyl-2,4-pentanediol BCC 45 v/v -
    • Ammonium acetate 0.2 M -
    		pH 5.5
    450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    200 μl of Ammonium acetate (1 M)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    250 μl of water (100 v/v)
    H6
    • BIS-TRIS 0.1 M pH 5.5
    • Ammonium acetate 0.1 M -
    • Polyethylene glycol 10,000 17 w/v -
    		pH 5.5
    100 μl of Ammonium acetate (1 M)
    340 μl of Polyethylene glycol 10,000 (50 w/v)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    460 μl of water (100 v/v)
    H7
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 3,350 25 w/v -
    • Ammonium sulfate 0.2 M -
    		pH 5.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    66 μl of Ammonium sulfate (3 M)
    333 μl of water (100 v/v)
    H8
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 3,350 25 w/v -
    • Sodium chloride 0.2 M -
    		pH 5.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    40 μl of Sodium chloride (5 M)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    360 μl of water (100 v/v)
    H9
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 3,350 25 w/v -
    • Lithium sulfate 0.2 M -
    		pH 5.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    80 μl of Lithium sulfate (2.5 M)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    320 μl of water (100 v/v)
    H10
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 3,350 25 w/v -
    • Ammonium acetate 0.2 M -
    		pH 5.5
    200 μl of Ammonium acetate (1 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    200 μl of water (100 v/v)
    H11
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 3,350 25 w/v -
    • Magnesium chloride 0.2 M -
    		pH 5.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    80 μl of Magnesium chloride (2.5 M)
    320 μl of water (100 v/v)
    H12
    • 2-methyl-2,4-pentanediol BCC 45 v/v -
    • HEPES 0.1 M pH 7.5
    • Ammonium acetate 0.2 M -
    		pH 7.5
    450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    200 μl of Ammonium acetate (1 M)
    100 μl of HEPES pH 7.5 (1 M)
    250 μl of water (100 v/v)