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Supplemental Information

Screen Description

JCSG+ HTS

JCSG / JCSG+ HTS
Created 2009-11-16.

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JCSG+ HTS

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Screen Structure

JCSG+ HTS

Well Count 96
Number of distinct ingredients 71
Internal diversity (score mean dist) 0.961

Screen Composition

2025-08-16T06:25:37.322304 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type JCSG+ HTS
Buffer 101
Cryoprotectant 6
Organic (non-volatile) 25
Organic (volatile) 6
Polymer 65
Salt 90

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
JCSG+ HTS Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Polyethylene glycol 400 50 v/v -
  • Sodium acetate 0.1 M pH 4.5
  • Lithium sulfate 0.2 M -
pH 4.5
80.0 μl of Lithium sulfate (2.5 M)
500.0 μl of Polyethylene glycol 400 (100 v/v)
50.0 μl of Sodium acetate pH 4.5 (2 M)
370.0 μl of water (100 v/v)
A2
  • Polyethylene glycol 3,000 20 w/v -
  • tri-Sodium citrate 0.1 M pH 5.5
pH 5.5
400.0 μl of Polyethylene glycol 3,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
500.0 μl of water (100 v/v)
A3
  • Polyethylene glycol 3,350 20 w/v -
  • di-Ammonium hydrogen citrate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
80.0 μl of di-Ammonium hydrogen citrate pH 5 (2.5 M)
520.0 μl of water (100 v/v)
A4
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
  • Sodium acetate 0.1 M pH 4.6
  • Calcium chloride 0.02 M -
pH 4.6
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
4.0 μl of Calcium chloride (5 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
596.0 μl of water (100 v/v)
A5
  • Polyethylene glycol 3,350 20 w/v -
  • Magnesium formate 0.2 M -
NA
200.0 μl of Magnesium formate pH 5.9 (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
A6
  • Potassium phosphate citrate 0.1 M pH 4.2
  • Polyethylene glycol 1,000 20 w/v -
  • Lithium sulfate 0.2 M -
pH 4.2
80.0 μl of Lithium sulfate (2.5 M)
400.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of Potassium phosphate citrate pH 4.2 (1 M)
420.0 μl of water (100 v/v)
A7
  • Polyethylene glycol 8,000 20 w/v -
  • CHES 0.1 M pH 9.5
pH 9.5
100.0 μl of CHES pH 9.5 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
500.0 μl of water (100 v/v)
A8
  • Polyethylene glycol 3,350 20 w/v -
  • Ammonium formate 0.2 M -
NA
40.0 μl of Ammonium formate pH 6.6 (5 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
560.0 μl of water (100 v/v)
A9
  • Polyethylene glycol 3,350 20 w/v -
  • Ammonium chloride 0.2 M -
NA
40.0 μl of Ammonium chloride pH 6.3 (5 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
560.0 μl of water (100 v/v)
A10
  • Polyethylene glycol 3,350 20 w/v -
  • Potassium formate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
14.3 μl of Potassium formate pH 7.3 (14 M)
585.7 μl of water (100 v/v)
A11
  • 2-methyl-2,4-pentanediol BCC 50 v/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium di-hydrogen phosphate 0.2 M -
pH 8.5
500.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
200.0 μl of Ammonium di-hydrogen phosphate (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
200.0 μl of water (100 v/v)
A12
  • Polyethylene glycol 3,350 20 w/v -
  • Potassium nitrate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
66.7 μl of Potassium nitrate pH 6.9 (3 M)
533.3 μl of water (100 v/v)
B1
  • tri-Sodium citrate 0.1 M pH 4
  • Ammonium sulfate 0.8 M -
pH 4
266.7 μl of Ammonium sulfate (3 M)
100.0 μl of tri-Sodium citrate pH 4 (1 M)
633.3 μl of water (100 v/v)
B2
  • Polyethylene glycol 3,350 20 w/v -
  • Sodium thiocyanate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
25.0 μl of Sodium thiocyanate pH 6.9 (8 M)
575.0 μl of water (100 v/v)
B3
  • Polyethylene glycol 6,000 20 w/v -
  • BICINE 0.1 M pH 9
pH 9
100.0 μl of BICINE pH 9 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
500.0 μl of water (100 v/v)
B4
  • Polyethylene glycol 8,000 10 w/v -
  • Ethylene glycol 8 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
80.0 μl of Ethylene glycol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
620.0 μl of water (100 v/v)
B5
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
  • Polyethylene glycol 8,000 5 w/v -
  • MES 0.1 M pH 6.5
pH 6.5
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of MES pH 6.5 (1 M)
100.0 μl of Polyethylene glycol 8,000 (50 w/v)
400.0 μl of water (100 v/v)
B6
  • Ethanol 40 v/v -
  • Polyethylene glycol 1,000 5 w/v -
  • Potassium phosphate citrate 0.1 M pH 4.2
pH 4.2
800.0 μl of Ethanol (50 v/v)
100.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of Potassium phosphate citrate pH 4.2 (1 M)
B7
  • Polyethylene glycol 4,000 8 w/v -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
160.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
740.0 μl of water (100 v/v)
B8
  • Polyethylene glycol 8,000 10 w/v -
  • tris chloride BCC 0.1 M pH 7
  • Magnesium chloride 0.2 M -
pH 7
80.0 μl of Magnesium chloride (2.5 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
33.3 μl of tris chloride BCC pH 7 (3 M)
686.7 μl of water (100 v/v)
B9
  • Polyethylene glycol 6,000 20 w/v -
  • tri-Sodium citrate 0.1 M pH 5
pH 5
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
500.0 μl of water (100 v/v)
B10
  • Polyethylene glycol 200 50 v/v -
  • MES 0.1 M pH 6.5
  • Magnesium chloride 0.2 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
500.0 μl of Polyethylene glycol 200 (100 v/v)
320.0 μl of water (100 v/v)
B11
  • tri-Sodium citrate 1.6 M -
NA
800.0 μl of tri-Sodium citrate (2 M)
200.0 μl of water (100 v/v)
B12
  • Polyethylene glycol 3,350 20 w/v -
  • tri-Potassium citrate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
80.0 μl of tri-Potassium citrate pH 8.3 (2.5 M)
520.0 μl of water (100 v/v)
C1
  • Polyethylene glycol 8,000 20 w/v -
  • Potassium phosphate citrate 0.1 M pH 4.2
  • Sodium chloride 0.2 M -
pH 4.2
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Potassium phosphate citrate pH 4.2 (1 M)
200.0 μl of Sodium chloride (1 M)
300.0 μl of water (100 v/v)
C2
  • Polyethylene glycol 6,000 20 w/v -
  • tri-Sodium citrate 0.1 M pH 4
  • Lithium chloride 1 M -
pH 4
200.0 μl of Lithium chloride (5 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 4 (1 M)
300.0 μl of water (100 v/v)
C3
  • Polyethylene glycol 3,350 20 w/v -
  • Ammonium nitrate 0.2 M -
NA
20.0 μl of Ammonium nitrate pH 6.3 (10 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
580.0 μl of water (100 v/v)
C4
  • Polyethylene glycol 6,000 10 w/v -
  • HEPES 0.1 M pH 7
pH 7
100.0 μl of HEPES pH 7 (1 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
700.0 μl of water (100 v/v)
C5
  • Sodium di-hydrogen phosphate 0.8 M -
  • Potassium di-hydrogen phosphate 0.8 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Potassium di-hydrogen phosphate (2 M)
200.0 μl of Sodium di-hydrogen phosphate (4 M)
300.0 μl of water (100 v/v)
C6
  • Polyethylene glycol 300 40 v/v -
  • Potassium phosphate citrate 0.1 M pH 4.2
pH 4.2
400.0 μl of Polyethylene glycol 300 (100 v/v)
100.0 μl of Potassium phosphate citrate pH 4.2 (1 M)
500.0 μl of water (100 v/v)
C7
  • Polyethylene glycol 3,000 10 w/v -
  • Sodium acetate 0.1 M pH 4.5
  • Zinc acetate 0.2 M -
pH 4.5
200.0 μl of Polyethylene glycol 3,000 (50 w/v)
50.0 μl of Sodium acetate pH 4.5 (2 M)
80.0 μl of Zinc acetate (2.5 M)
670.0 μl of water (100 v/v)
C8
  • Ethanol 20 v/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
400.0 μl of Ethanol (50 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
C9
  • 1,2-Propanediol 25 v/v -
  • Glycerol 10 v/v -
  • Sodium Potassium phosphate 0.1 M pH 6.2
pH 6.2
250.0 μl of 1,2-Propanediol (100 v/v)
100.0 μl of Glycerol (100 v/v)
25.0 μl of Sodium Potassium phosphate pH 6.2 (4 M)
625.0 μl of water (100 v/v)
C10
  • Polyethylene glycol 20,000 10 w/v -
  • BICINE 0.1 M pH 9
  • 1,4-Dioxane 2 v/v -
pH 9
40.0 μl of 1,4-Dioxane (50 v/v)
100.0 μl of BICINE pH 9 (1 M)
200.0 μl of Polyethylene glycol 20,000 (50 w/v)
660.0 μl of water (100 v/v)
C11
  • Sodium acetate 0.1 M pH 4.6
  • Ammonium sulfate 2 M -
pH 4.6
666.7 μl of Ammonium sulfate (3 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
233.3 μl of water (100 v/v)
C12
  • Polyethylene glycol 1,000 10 w/v -
  • Polyethylene glycol 8,000 10 w/v -
NA
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
600.0 μl of water (100 v/v)
D1
  • Polyethylene glycol 1,500 24 w/v -
  • Glycerol 20 v/v -
NA
200.0 μl of Glycerol (100 v/v)
480.0 μl of Polyethylene glycol 1,500 (50 w/v)
320.0 μl of water (100 v/v)
D2
  • Polyethylene glycol 400 30 v/v -
  • HEPES 0.1 M pH 7.5
  • Magnesium chloride 0.2 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
520.0 μl of water (100 v/v)
D3
  • Polyethylene glycol 200 50 v/v -
  • Sodium Potassium phosphate 0.1 M pH 6.2
  • Sodium chloride 0.2 M -
pH 6.2
500.0 μl of Polyethylene glycol 200 (100 v/v)
25.0 μl of Sodium Potassium phosphate pH 6.2 (4 M)
40.0 μl of Sodium chloride (5 M)
435.0 μl of water (100 v/v)
D4
  • Polyethylene glycol 8,000 30 w/v -
  • Sodium acetate 0.1 M pH 4.5
  • Lithium sulfate 0.2 M -
pH 4.5
80.0 μl of Lithium sulfate (2.5 M)
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
50.0 μl of Sodium acetate pH 4.5 (2 M)
270.0 μl of water (100 v/v)
D5
  • 2-methyl-2,4-pentanediol BCC 70 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
700.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of water (100 v/v)
D6
  • Polyethylene glycol 8,000 20 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Magnesium chloride 0.2 M -
pH 8.5
80.0 μl of Magnesium chloride (2.5 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
420.0 μl of water (100 v/v)
D7
  • Polyethylene glycol 400 40 v/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Lithium sulfate 0.2 M -
pH 8.5
80.0 μl of Lithium sulfate (2.5 M)
400.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
420.0 μl of water (100 v/v)
D8
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
  • tris chloride BCC 0.1 M pH 8
pH 8
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
500.0 μl of water (100 v/v)
D9
  • Polyethylene glycol 4,000 25.5 w/v -
  • Glycerol 15 v/v -
  • Ammonium sulfate 0.17 M -
NA
56.7 μl of Ammonium sulfate (3 M)
150.0 μl of Glycerol (100 v/v)
510.0 μl of Polyethylene glycol 4,000 (50 w/v)
283.3 μl of water (100 v/v)
D10
  • Polyethylene glycol 300 40 v/v -
  • MES 0.1 M pH 6.5
  • Calcium acetate 0.2 M -
pH 6.5
100.0 μl of Calcium acetate (2 M)
33.3 μl of MES pH 6.5 (3 M)
400.0 μl of Polyethylene glycol 300 (100 v/v)
466.7 μl of water (100 v/v)
D11
  • 2-Propanol 14 v/v -
  • Glycerol 30 v/v -
  • Sodium acetate 0.07 M pH 4.6
  • Calcium chloride 0.14 M -
pH 4.6
140.0 μl of 2-Propanol (100 v/v)
28.0 μl of Calcium chloride (5 M)
300.0 μl of Glycerol (100 v/v)
70.0 μl of Sodium acetate pH 4.6 (1 M)
462.0 μl of water (100 v/v)
D12
  • Polyethylene glycol 8,000 16 w/v -
  • Glycerol 20 v/v -
  • Potassium di-hydrogen phosphate 0.04 M -
NA
200.0 μl of Glycerol (100 v/v)
320.0 μl of Polyethylene glycol 8,000 (50 w/v)
20.0 μl of Potassium di-hydrogen phosphate (2 M)
460.0 μl of water (100 v/v)
E1
  • MES 0.1 M pH 6.5
  • tri-Sodium citrate 1 M -
pH 6.5
33.3 μl of MES pH 6.5 (3 M)
500.0 μl of tri-Sodium citrate (2 M)
466.7 μl of water (100 v/v)
E2
  • Ammonium sulfate 2 M -
  • MES 0.1 M pH 6.5
  • Sodium chloride 0.2 M -
pH 6.5
400.0 μl of Ammonium sulfate (5 M)
33.3 μl of MES pH 6.5 (3 M)
40.0 μl of Sodium chloride (5 M)
526.7 μl of water (100 v/v)
E3
  • 2-Propanol 10 v/v -
  • HEPES 0.1 M pH 7.5
  • Sodium chloride 0.2 M -
pH 7.5
100.0 μl of 2-Propanol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
40.0 μl of Sodium chloride (5 M)
760.0 μl of water (100 v/v)
E4
  • Ammonium sulfate 1.26 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Lithium sulfate 0.2 M -
pH 8.5
420.0 μl of Ammonium sulfate (3 M)
80.0 μl of Lithium sulfate (2.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
E5
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
  • CAPS 0.1 M pH 10
pH 10
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of CAPS pH 10 (1 M)
500.0 μl of water (100 v/v)
E6
  • Polyethylene glycol 3,000 20 w/v -
  • Imidazole 0.1 M pH 8
  • Zinc acetate 0.2 M -
pH 8
100.0 μl of Imidazole pH 8 (1 M)
400.0 μl of Polyethylene glycol 3,000 (50 w/v)
100.0 μl of Zinc acetate (2 M)
400.0 μl of water (100 v/v)
E7
  • 2-Propanol 10 v/v -
  • MES 0.1 M pH 6.5
  • Zinc acetate 0.2 M -
pH 6.5
100.0 μl of 2-Propanol (100 v/v)
33.3 μl of MES pH 6.5 (3 M)
100.0 μl of Zinc acetate (2 M)
766.7 μl of water (100 v/v)
E8
  • Sodium acetate 0.1 M pH 4.5
  • di-Ammonium hydrogen phosphate 1 M -
pH 4.5
33.3 μl of Sodium acetate pH 4.5 (3 M)
285.7 μl of di-Ammonium hydrogen phosphate (3.5 M)
681.0 μl of water (100 v/v)
E9
  • MES 0.1 M pH 6.5
  • Magnesium sulfate 1.6 M -
pH 6.5
33.3 μl of MES pH 6.5 (3 M)
800.0 μl of Magnesium sulfate (2 M)
166.7 μl of water (100 v/v)
E10
  • Polyethylene glycol 6,000 10 w/v -
  • BICINE 0.1 M pH 9
pH 9
100.0 μl of BICINE pH 9 (1 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
700.0 μl of water (100 v/v)
E11
  • Polyethylene glycol 8,000 14.4 w/v -
  • Glycerol 20 v/v -
  • MES 0.08 M pH 6.5
  • Calcium acetate 0.16 M -
pH 6.5
160.0 μl of Calcium acetate (1 M)
200.0 μl of Glycerol (100 v/v)
26.7 μl of MES pH 6.5 (3 M)
288.0 μl of Polyethylene glycol 8,000 (50 w/v)
325.3 μl of water (100 v/v)
E12
  • Polyethylene glycol 8,000 10 w/v -
  • Imidazole 0.1 M pH 8
pH 8
100.0 μl of Imidazole pH 8 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
700.0 μl of water (100 v/v)
F1
  • Jeffamine® M-600 30 v/v -
  • MES 0.1 M pH 6.5
  • Cesium chloride 0.05 M -
pH 6.5
50.0 μl of Cesium chloride (1 M)
600.0 μl of Jeffamine® M-600 (50 v/v)
33.3 μl of MES pH 6.5 (3 M)
316.7 μl of water (100 v/v)
F2
  • tri-Sodium citrate 0.1 M pH 5
  • Ammonium sulfate 3.15 M -
pH 5
787.5 μl of Ammonium sulfate (4 M)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
112.5 μl of water (100 v/v)
F3
  • 2-methyl-2,4-pentanediol BCC 20 v/v -
  • tris chloride BCC 0.1 M pH 8
pH 8
200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
700.0 μl of water (100 v/v)
F4
  • Jeffamine® M-600 20 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Jeffamine® M-600 (50 v/v)
500.0 μl of water (100 v/v)
F5
  • Ethylene glycol 50 v/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Magnesium chloride 0.2 M -
pH 8.5
500.0 μl of Ethylene glycol (100 v/v)
80.0 μl of Magnesium chloride (2.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
320.0 μl of water (100 v/v)
F6
  • 2-methyl-2,4-pentanediol BCC 10 v/v -
  • BICINE 0.1 M pH 9
pH 9
100.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of BICINE pH 9 (1 M)
800.0 μl of water (100 v/v)
F7
  • di-Sodium succinate 0.8 M -
NA
266.7 μl of di-Sodium succinate pH 7 (3 M)
733.3 μl of water (100 v/v)
F8
  • di-Sodium DL-malate 2.1 M -
NA
700.0 μl of di-Sodium DL-malate pH 7 (3 M)
300.0 μl of water (100 v/v)
F9
  • di-Sodium malonate 2.4 M -
NA
800.0 μl of di-Sodium malonate pH 7 (3 M)
200.0 μl of water (100 v/v)
F10
  • di-Sodium malonate 1.1 M -
  • Jeffamine® ED-2001 0.5 w/v -
  • HEPES 0.1 M pH 7
pH 7
100.0 μl of HEPES pH 7 (1 M)
10.0 μl of Jeffamine® ED-2001 pH 7 (50 w/v)
366.7 μl of di-Sodium malonate pH 7 (3 M)
523.3 μl of water (100 v/v)
F11
  • Polyethylene glycol monomethyl ether 2,000 1 w/v -
  • HEPES 0.1 M pH 7
  • di-Sodium succinate 1 M -
pH 7
100.0 μl of HEPES pH 7 (1 M)
20.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
333.3 μl of di-Sodium succinate pH 7 (3 M)
546.7 μl of water (100 v/v)
F12
  • Jeffamine® M-600 30 v/v -
  • HEPES 0.1 M pH 7
pH 7
100.0 μl of HEPES pH 7 (1 M)
600.0 μl of Jeffamine® M-600 pH 7 (50 v/v)
300.0 μl of water (100 v/v)
G1
  • Jeffamine® ED-2001 30 w/v -
  • HEPES 0.1 M pH 7
pH 7
100.0 μl of HEPES pH 7 (1 M)
600.0 μl of Jeffamine® ED-2001 pH 7 (50 w/v)
300.0 μl of water (100 v/v)
G2
  • Poly(acrylic acid sodium salt) 5,100 22 w/v -
  • HEPES 0.1 M pH 7.5
  • Magnesium chloride 0.02 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
8.0 μl of Magnesium chloride (2.5 M)
440.0 μl of Poly(acrylic acid sodium salt) 5,100 (50 w/v)
452.0 μl of water (100 v/v)
G3
  • Polyvinylpyrrolidone K15 20 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Cobalt (II) chloride 0.1 M -
pH 8.5
100.0 μl of Cobalt (II) chloride (1 M)
400.0 μl of Polyvinylpyrrolidone K15 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
G4
  • Polyethylene glycol 2,000 20 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Trimethylamine N-oxide 0.2 M -
pH 8.5
400.0 μl of Polyethylene glycol 2,000 (50 w/v)
200.0 μl of Trimethylamine N-oxide (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
G5
  • Polyethylene glycol 3,350 12 w/v -
  • Cobalt (II) chloride 0.005 M -
  • Cadmium chloride 0.005 M -
  • Nickel (II) chloride 0.005 M -
  • Magnesium chloride 0.005 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
5.0 μl of Cadmium chloride (1 M)
5.0 μl of Cobalt (II) chloride (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
2.0 μl of Magnesium chloride (2.5 M)
5.0 μl of Nickel (II) chloride (1 M)
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
643.0 μl of water (100 v/v)
G6
  • Polyethylene glycol 3,350 20 w/v -
  • di-Sodium malonate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
66.7 μl of di-Sodium malonate pH 7 (3 M)
533.3 μl of water (100 v/v)
G7
  • Polyethylene glycol 3,350 15 w/v -
  • di-Sodium succinate 0.1 M -
NA
300.0 μl of Polyethylene glycol 3,350 (50 w/v)
33.3 μl of di-Sodium succinate pH 7 (3 M)
666.7 μl of water (100 v/v)
G8
  • Polyethylene glycol 3,350 20 w/v -
  • di-Sodium DL-malate 0.15 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
50.0 μl of di-Sodium DL-malate pH 7 (3 M)
550.0 μl of water (100 v/v)
G9
  • Polyethylene glycol monomethyl ether 2,000 30 w/v -
  • Potassium thiocyanate 0.1 M -
NA
600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
12.5 μl of Potassium thiocyanate (8 M)
387.5 μl of water (100 v/v)
G10
  • Polyethylene glycol monomethyl ether 2,000 30 w/v -
  • Potassium bromide 0.15 M -
NA
600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
37.5 μl of Potassium bromide (4 M)
362.5 μl of water (100 v/v)
G11
  • BIS-TRIS 0.1 M pH 5.5
  • Ammonium sulfate 2 M -
pH 5.5
666.7 μl of Ammonium sulfate (3 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
233.3 μl of water (100 v/v)
G12
  • BIS-TRIS 0.1 M pH 5.5
  • Sodium chloride 3 M -
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
600.0 μl of Sodium chloride (5 M)
300.0 μl of water (100 v/v)
H1
  • BIS-TRIS 0.1 M pH 5.5
  • Magnesium formate 0.3 M -
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
300.0 μl of Magnesium formate (1 M)
600.0 μl of water (100 v/v)
H2
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 1 w/v -
  • Ammonium sulfate 1 M -
pH 5.5
333.3 μl of Ammonium sulfate (3 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
20.0 μl of Polyethylene glycol 3,350 (50 w/v)
546.7 μl of water (100 v/v)
H3
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 25 w/v -
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
H4
  • BIS-TRIS 0.1 M pH 5.5
  • 2-methyl-2,4-pentanediol BCC 45 v/v -
  • Calcium chloride 0.2 M -
pH 5.5
450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
200.0 μl of Calcium chloride (1 M)
250.0 μl of water (100 v/v)
H5
  • BIS-TRIS 0.1 M pH 5.5
  • 2-methyl-2,4-pentanediol BCC 45 v/v -
  • Ammonium acetate 0.2 M -
pH 5.5
450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
200.0 μl of Ammonium acetate (1 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
250.0 μl of water (100 v/v)
H6
  • BIS-TRIS 0.1 M pH 5.5
  • Ammonium acetate 0.1 M -
  • Polyethylene glycol 10,000 17 w/v -
pH 5.5
100.0 μl of Ammonium acetate (1 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
340.0 μl of Polyethylene glycol 10,000 (50 w/v)
460.0 μl of water (100 v/v)
H7
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 25 w/v -
  • Ammonium sulfate 0.2 M -
pH 5.5
66.7 μl of Ammonium sulfate (3 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
333.3 μl of water (100 v/v)
H8
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 25 w/v -
  • Sodium chloride 0.2 M -
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Sodium chloride (5 M)
360.0 μl of water (100 v/v)
H9
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 25 w/v -
  • Lithium sulfate 0.2 M -
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
80.0 μl of Lithium sulfate (2.5 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
320.0 μl of water (100 v/v)
H10
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 25 w/v -
  • Ammonium acetate 0.2 M -
pH 5.5
200.0 μl of Ammonium acetate (1 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of water (100 v/v)
H11
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 25 w/v -
  • Magnesium chloride 0.2 M -
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
320.0 μl of water (100 v/v)
H12
  • 2-methyl-2,4-pentanediol BCC 45 v/v -
  • HEPES 0.1 M pH 7.5
  • Ammonium acetate 0.2 M -
pH 7.5
450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
200.0 μl of Ammonium acetate (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
250.0 μl of water (100 v/v)