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Supplemental Information

Screen Description

Kinase 1

Kinase / Kinase 1
Created 2009-11-16.

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Kinase 1

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Kinase 1

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Screen Structure

Kinase 1

Well Count 24
Number of distinct ingredients 31
Internal diversity (score mean dist) 0.917

Screen Composition

More information about screen composition chart.
2025-08-16T09:05:39.572544 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Kinase 1
Buffer 32
Cryoprotectant 2
Organic (non-volatile) 5
Polymer 3
Salt 40

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Kinase 1 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Magnesium acetate 0.2 M -
  • Dithiothreitol 0.01 M -
  • Ammonium sulfate 1 M -
  • tri-Sodium citrate 0.1 M pH 5.6
pH 5.6
333.3 μl of Ammonium sulfate (3 M)
100.0 μl of Dithiothreitol (0.1 M)
100.0 μl of Magnesium acetate (2 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
366.7 μl of water (100 v/v)
A2
  • tri-Sodium citrate 0.1 M pH 5.6
  • Ammonium sulfate 1.3 M -
pH 5.6
433.3 μl of Ammonium sulfate (3 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
466.7 μl of water (100 v/v)
A3
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium sulfate 1.3 M -
pH 8.5
433.3 μl of Ammonium sulfate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
466.7 μl of water (100 v/v)
A4
  • Cobalt (II) chloride 0.025 M -
  • MES 0.1 M pH 6.5
  • Ammonium sulfate 1.8 M -
pH 6.5
600.0 μl of Ammonium sulfate (3 M)
25.0 μl of Cobalt (II) chloride (1 M)
100.0 μl of MES pH 6.5 (1 M)
275.0 μl of water (100 v/v)
A5
  • Sodium chloride 0.2 M -
  • tri-Sodium citrate 0.1 M pH 3.1
  • Ammonium sulfate 2 M -
pH 3.1
666.7 μl of Ammonium sulfate (3 M)
200.0 μl of Sodium chloride (1 M)
100.0 μl of tri-Sodium citrate pH 3.1 (1 M)
33.3 μl of water (100 v/v)
A6
  • Magnesium chloride 0.05 M -
  • Sodium acetate 0.1 M pH 4.6
  • Ammonium sulfate 2 M -
pH 4.6
666.7 μl of Ammonium sulfate (3 M)
20.0 μl of Magnesium chloride (2.5 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
213.3 μl of water (100 v/v)
B1
  • Sodium acetate 0.1 M pH 4.6
  • Ammonium sulfate 2 M -
pH 4.6
666.7 μl of Ammonium sulfate (3 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
233.3 μl of water (100 v/v)
B2
  • Polyethylene glycol monomethyl ether 550 2 v/v -
  • HEPES 0.1 M pH 7.5
  • Ammonium sulfate 2 M -
pH 7.5
666.7 μl of Ammonium sulfate (3 M)
100.0 μl of HEPES pH 7.5 (1 M)
40.0 μl of Polyethylene glycol monomethyl ether 550 (50 v/v)
193.3 μl of water (100 v/v)
B3
  • HEPES 0.1 M pH 7.5
  • Ammonium sulfate 2 M -
pH 7.5
666.7 μl of Ammonium sulfate (3 M)
100.0 μl of HEPES pH 7.5 (1 M)
233.3 μl of water (100 v/v)
B4
  • Magnesium chloride 0.006 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium sulfate 2 M -
pH 8.5
666.7 μl of Ammonium sulfate (3 M)
2.4 μl of Magnesium chloride (2.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
230.9 μl of water (100 v/v)
B5
  • Nickel sulfate 0.01 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Lithium sulfate 1.5 M -
pH 8.5
500.0 μl of Lithium sulfate (3 M)
2.5 μl of Nickel sulfate (4 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
397.5 μl of water (100 v/v)
B6
  • tri-Sodium citrate 0.1 M pH 4.2
  • Lithium chloride 1 M -
pH 4.2
200.0 μl of Lithium chloride (5 M)
100.0 μl of tri-Sodium citrate pH 4.2 (1 M)
700.0 μl of water (100 v/v)
C1
  • Sodium acetate 0.1 M pH 4.6
  • Sodium chloride 2 M -
pH 4.6
100.0 μl of Sodium acetate pH 4.6 (1 M)
400.0 μl of Sodium chloride (5 M)
500.0 μl of water (100 v/v)
C2
  • di-Sodium hydrogen phosphate 0.1 M -
  • MES 0.1 M pH 6.5
  • Sodium chloride 2 M -
  • Potassium di-hydrogen phosphate 0.1 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
50.0 μl of Potassium di-hydrogen phosphate (2 M)
400.0 μl of Sodium chloride (5 M)
20.0 μl of di-Sodium hydrogen phosphate (5 M)
430.0 μl of water (100 v/v)
C3
  • Glycerol 1 v/v -
  • HEPES 0.1 M pH 7.5
  • Sodium chloride 3.3 M -
pH 7.5
10.0 μl of Glycerol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
660.0 μl of Sodium chloride (5 M)
230.0 μl of water (100 v/v)
C4
  • Calcium chloride 0.00625 M -
  • MES 0.1 M pH 6.5
  • Sodium acetate 1.2 M -
pH 6.5
6.2 μl of Calcium chloride (1 M)
100.0 μl of MES pH 6.5 (1 M)
600.0 μl of Sodium acetate (2 M)
293.8 μl of water (100 v/v)
C5
  • Polyethylene glycol 3,000 2 w/v -
  • BICINE 0.1 M pH 9.5
  • Sodium formate 3.7 M -
pH 9.5
100.0 μl of BICINE pH 9.5 (1 M)
40.0 μl of Polyethylene glycol 3,000 (50 w/v)
528.6 μl of Sodium formate (7 M)
331.4 μl of water (100 v/v)
C6
  • Glycerol 1.6 v/v -
  • Dithiothreitol 0.01 M -
  • di-Sodium malonate 0.5 M -
  • PIPES 0.05 M pH 6
pH 6
100.0 μl of Dithiothreitol (0.1 M)
16.0 μl of Glycerol (100 v/v)
50.0 μl of PIPES pH 6 (1 M)
166.7 μl of di-Sodium malonate pH 6 (3 M)
667.3 μl of water (100 v/v)
D1
  • Potassium di-hydrogen phosphate 0.5 M -
  • Lithium sulfate 0.2 M -
  • di-Sodium hydrogen phosphate 0.5 M -
  • CAPS 0.1 M pH 10
pH 10
100.0 μl of CAPS pH 10 (1 M)
66.7 μl of Lithium sulfate (3 M)
250.0 μl of Potassium di-hydrogen phosphate (2 M)
100.0 μl of di-Sodium hydrogen phosphate (5 M)
483.3 μl of water (100 v/v)
D2
  • Dithiothreitol 0.005 M -
  • tris chloride BCC 0.1 M pH 8.5
  • di-Sodium tartrate 1.2 M -
pH 8.5
50.0 μl of Dithiothreitol (0.1 M)
600.0 μl of di-Sodium tartrate (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
250.0 μl of water (100 v/v)
D3
  • MES 0.1 M pH 6.5
  • Potassium Sodium tartrate 1 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
500.0 μl of Potassium Sodium tartrate (2 M)
400.0 μl of water (100 v/v)
D4
  • Cesium chloride 0.05 M -
  • MES 0.1 M pH 6.5
  • Jeffamine® M-600 30 v/v -
pH 6.5
50.0 μl of Cesium chloride (1 M)
600.0 μl of Jeffamine® M-600 (50 v/v)
100.0 μl of MES pH 6.5 (1 M)
250.0 μl of water (100 v/v)
D5
  • MES 0.1 M pH 6.5
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
pH 6.5
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of MES pH 6.5 (1 M)
500.0 μl of water (100 v/v)
D6
  • HEPES 0.1 M pH 7.5
  • 2-methyl-2,4-pentanediol BCC 50 v/v -
pH 7.5
500.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of water (100 v/v)