C6 - Kinase 1 - Screen Contents

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Supplemental Information

Screen Description

JB Kinase / Kinase 1

Created 2009-11-16.

Rigaku Recipe XML

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Screen Structure

Well Count	24
Number of distinct ingredients	31
Internal diversity (Score mean dist)	0.92
Internal diversity (Ion mean dist)	0.89
Internal diversity (PEG mean dist)	0.89
Internal diversity (Known pH mean dist)	0.79
Internal diversity (Penalty mean dist)	0.9

Screen Composition

More information about screen composition chart.

Chemical classes bar chart — ingredient type Kinase 1

Buffer 32

Cryoprotectant 2

Organic (non-volatile) 5

Polymer 3

Salt 40

ingredient type	Kinase 1
Buffer	32
Cryoprotectant	2
Organic (non-volatile)	5
Polymer	3
Salt	40

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Kinase 1 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Ammonium sulfate 1 M - Magnesium acetate 0.2 M - Dithiothreitol 0.01 M - tri-Sodium citrate 0.1 M pH 5.6 🔍 ⚖️	pH 5.6	333.33 μl of Ammonium sulfate (3 M) 100.0 μl of Dithiothreitol (0.1 M) 100.0 μl of Magnesium acetate (2 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 366.67 μl of water (100 v/v)
A2	Ammonium sulfate 1.3 M - tri-Sodium citrate 0.1 M pH 5.6 🔍 ⚖️	pH 5.6	433.33 μl of Ammonium sulfate (3 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 466.67 μl of water (100 v/v)
A3	Ammonium sulfate 1.3 M - tris chloride BCC 0.1 M pH 8.5 🔍 ⚖️	pH 8.5	433.33 μl of Ammonium sulfate (3 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 466.67 μl of water (100 v/v)
A4	Ammonium sulfate 1.8 M - Cobalt (II) chloride 0.025 M - MES 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	600.0 μl of Ammonium sulfate (3 M) 25.0 μl of Cobalt (II) chloride (1 M) 100.0 μl of MES pH 6.5 (1 M) 275.0 μl of water (100 v/v)
A5	Ammonium sulfate 2 M - Sodium chloride 0.2 M - tri-Sodium citrate 0.1 M pH 3.1 🔍 ⚖️	pH 3.1	666.67 μl of Ammonium sulfate (3 M) 200.0 μl of Sodium chloride (1 M) 100.0 μl of tri-Sodium citrate pH 3.1 (1 M) 33.33 μl of water (100 v/v)
A6	Ammonium sulfate 2 M - Magnesium chloride 0.05 M - Sodium acetate 0.1 M pH 4.6 🔍 ⚖️	pH 4.6	666.67 μl of Ammonium sulfate (3 M) 20.0 μl of Magnesium chloride (2.5 M) 100.0 μl of Sodium acetate pH 4.6 (1 M) 213.33 μl of water (100 v/v)
B1	Ammonium sulfate 2 M - Sodium acetate 0.1 M pH 4.6 🔍 ⚖️	pH 4.6	666.67 μl of Ammonium sulfate (3 M) 100.0 μl of Sodium acetate pH 4.6 (1 M) 233.33 μl of water (100 v/v)
B2	Ammonium sulfate 2 M - Polyethylene glycol monomethyl ether 550 2 v/v - HEPES 0.1 M pH 7.5 🔍 ⚖️	pH 7.5	666.67 μl of Ammonium sulfate (3 M) 100.0 μl of HEPES pH 7.5 (1 M) 40.0 μl of Polyethylene glycol monomethyl ether 550 (50 v/v) 193.33 μl of water (100 v/v)
B3	Ammonium sulfate 2 M - HEPES 0.1 M pH 7.5 🔍 ⚖️	pH 7.5	666.67 μl of Ammonium sulfate (3 M) 100.0 μl of HEPES pH 7.5 (1 M) 233.33 μl of water (100 v/v)
B4	Ammonium sulfate 2 M - Magnesium chloride 0.006 M - tris chloride BCC 0.1 M pH 8.5 🔍 ⚖️	pH 8.5	666.67 μl of Ammonium sulfate (3 M) 2.4 μl of Magnesium chloride (2.5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 230.93 μl of water (100 v/v)
B5	Nickel sulfate 0.01 M - Lithium sulfate 1.5 M - tris chloride BCC 0.1 M pH 8.5 🔍 ⚖️	pH 8.5	500.0 μl of Lithium sulfate (3 M) 2.5 μl of Nickel sulfate (4 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 397.5 μl of water (100 v/v)
B6	Lithium chloride 1 M - tri-Sodium citrate 0.1 M pH 4.2 🔍 ⚖️	pH 4.2	200.0 μl of Lithium chloride (5 M) 100.0 μl of tri-Sodium citrate pH 4.2 (1 M) 700.0 μl of water (100 v/v)
C1	Sodium chloride 2 M - Sodium acetate 0.1 M pH 4.6 🔍 ⚖️	pH 4.6	100.0 μl of Sodium acetate pH 4.6 (1 M) 400.0 μl of Sodium chloride (5 M) 500.0 μl of water (100 v/v)
C2	Sodium chloride 2 M - di-Sodium hydrogen phosphate 0.1 M - Potassium di-hydrogen phosphate 0.1 M - MES 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	20.0 μl of di-Sodium hydrogen phosphate (5 M) 100.0 μl of MES pH 6.5 (1 M) 50.0 μl of Potassium di-hydrogen phosphate (2 M) 400.0 μl of Sodium chloride (5 M) 430.0 μl of water (100 v/v)
C3	Sodium chloride 3.3 M - Glycerol 1 v/v - HEPES 0.1 M pH 7.5 🔍 ⚖️	pH 7.5	10.0 μl of Glycerol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 660.0 μl of Sodium chloride (5 M) 230.0 μl of water (100 v/v)
C4	Sodium acetate 1.2 M - Calcium chloride 0.00625 M - MES 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	6.25 μl of Calcium chloride (1 M) 100.0 μl of MES pH 6.5 (1 M) 600.0 μl of Sodium acetate (2 M) 293.75 μl of water (100 v/v)
C5	Sodium formate 3.7 M - Polyethylene glycol 3,000 2 w/v - BICINE 0.1 M pH 9.5 🔍 ⚖️	pH 9.5	100.0 μl of BICINE pH 9.5 (1 M) 40.0 μl of Polyethylene glycol 3,000 (50 w/v) 528.57 μl of Sodium formate (7 M) 331.43 μl of water (100 v/v)
C6	di-Sodium malonate 0.5 M - Glycerol 1.6 v/v - Dithiothreitol 0.01 M - PIPES 0.05 M pH 6 🔍 ⚖️	pH 6	166.67 μl of di-Sodium malonate pH 6 (3 M) 100.0 μl of Dithiothreitol (0.1 M) 16.0 μl of Glycerol (100 v/v) 50.0 μl of PIPES pH 6 (1 M) 667.33 μl of water (100 v/v)
D1	di-Sodium hydrogen phosphate 0.5 M - Potassium di-hydrogen phosphate 0.5 M - Lithium sulfate 0.2 M - CAPS 0.1 M pH 10 🔍 ⚖️	pH 10	100.0 μl of CAPS pH 10 (1 M) 100.0 μl of di-Sodium hydrogen phosphate (5 M) 66.67 μl of Lithium sulfate (3 M) 250.0 μl of Potassium di-hydrogen phosphate (2 M) 483.33 μl of water (100 v/v)
D2	di-Sodium tartrate 1.2 M - Dithiothreitol 0.005 M - tris chloride BCC 0.1 M pH 8.5 🔍 ⚖️	pH 8.5	600.0 μl of di-Sodium tartrate (2 M) 50.0 μl of Dithiothreitol (0.1 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 250.0 μl of water (100 v/v)
D3	Potassium Sodium tartrate 1 M - MES 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 500.0 μl of Potassium Sodium tartrate (2 M) 400.0 μl of water (100 v/v)
D4	Jeffamine® M-600 30 v/v - Cesium chloride 0.05 M - MES 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	50.0 μl of Cesium chloride (1 M) 600.0 μl of Jeffamine® M-600 (50 v/v) 100.0 μl of MES pH 6.5 (1 M) 250.0 μl of water (100 v/v)
D5	2-methyl-2,4-pentanediol BCC 40 v/v - MES 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of MES pH 6.5 (1 M) 500.0 μl of water (100 v/v)
D6	2-methyl-2,4-pentanediol BCC 50 v/v - HEPES 0.1 M pH 7.5 🔍 ⚖️	pH 7.5	500.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of water (100 v/v)