Welcome to the new C6 website. This website aims preserve as many features as possible of the original C6 website.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
Kinase / Kinase 1
Created 2009-11-16.
Rigaku Recipe XML
Export
Barcodes
No barcodes found.
Screen Structure
Well Count | 24 |
---|---|
Number of distinct ingredients | 31 |
Internal diversity (score mean dist) | 0.917 |
Screen Composition
ingredient type | Kinase 1 |
---|---|
Buffer | 32 |
Cryoprotectant | 2 |
Organic (non-volatile) | 5 |
Polymer | 3 |
Salt | 40 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 5.6 | 333.3 μl of Ammonium sulfate (3 M) 100.0 μl of Dithiothreitol (0.1 M) 100.0 μl of Magnesium acetate (2 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 366.7 μl of water (100 v/v) |
A2 |
| pH 5.6 | 433.3 μl of Ammonium sulfate (3 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 466.7 μl of water (100 v/v) |
A3 |
| pH 8.5 | 433.3 μl of Ammonium sulfate (3 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 466.7 μl of water (100 v/v) |
A4 |
| pH 6.5 | 600.0 μl of Ammonium sulfate (3 M) 25.0 μl of Cobalt (II) chloride (1 M) 100.0 μl of MES pH 6.5 (1 M) 275.0 μl of water (100 v/v) |
A5 |
| pH 3.1 | 666.7 μl of Ammonium sulfate (3 M) 200.0 μl of Sodium chloride (1 M) 100.0 μl of tri-Sodium citrate pH 3.1 (1 M) 33.3 μl of water (100 v/v) |
A6 |
| pH 4.6 | 666.7 μl of Ammonium sulfate (3 M) 20.0 μl of Magnesium chloride (2.5 M) 100.0 μl of Sodium acetate pH 4.6 (1 M) 213.3 μl of water (100 v/v) |
B1 |
| pH 4.6 | 666.7 μl of Ammonium sulfate (3 M) 100.0 μl of Sodium acetate pH 4.6 (1 M) 233.3 μl of water (100 v/v) |
B2 |
| pH 7.5 | 666.7 μl of Ammonium sulfate (3 M) 100.0 μl of HEPES pH 7.5 (1 M) 40.0 μl of Polyethylene glycol monomethyl ether 550 (50 v/v) 193.3 μl of water (100 v/v) |
B3 |
| pH 7.5 | 666.7 μl of Ammonium sulfate (3 M) 100.0 μl of HEPES pH 7.5 (1 M) 233.3 μl of water (100 v/v) |
B4 |
| pH 8.5 | 666.7 μl of Ammonium sulfate (3 M) 2.4 μl of Magnesium chloride (2.5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 230.9 μl of water (100 v/v) |
B5 |
| pH 8.5 | 500.0 μl of Lithium sulfate (3 M) 2.5 μl of Nickel sulfate (4 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 397.5 μl of water (100 v/v) |
B6 |
| pH 4.2 | 200.0 μl of Lithium chloride (5 M) 100.0 μl of tri-Sodium citrate pH 4.2 (1 M) 700.0 μl of water (100 v/v) |
C1 |
| pH 4.6 | 100.0 μl of Sodium acetate pH 4.6 (1 M) 400.0 μl of Sodium chloride (5 M) 500.0 μl of water (100 v/v) |
C2 |
| pH 6.5 | 20.0 μl of di-Sodium hydrogen phosphate (5 M) 100.0 μl of MES pH 6.5 (1 M) 50.0 μl of Potassium di-hydrogen phosphate (2 M) 400.0 μl of Sodium chloride (5 M) 430.0 μl of water (100 v/v) |
C3 |
| pH 7.5 | 10.0 μl of Glycerol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 660.0 μl of Sodium chloride (5 M) 230.0 μl of water (100 v/v) |
C4 |
| pH 6.5 | 6.2 μl of Calcium chloride (1 M) 100.0 μl of MES pH 6.5 (1 M) 600.0 μl of Sodium acetate (2 M) 293.8 μl of water (100 v/v) |
C5 |
| pH 9.5 | 100.0 μl of BICINE pH 9.5 (1 M) 40.0 μl of Polyethylene glycol 3,000 (50 w/v) 528.6 μl of Sodium formate (7 M) 331.4 μl of water (100 v/v) |
C6 |
| pH 6 | 166.7 μl of di-Sodium malonate pH 6 (3 M) 100.0 μl of Dithiothreitol (0.1 M) 16.0 μl of Glycerol (100 v/v) 50.0 μl of PIPES pH 6 (1 M) 667.3 μl of water (100 v/v) |
D1 |
| pH 10 | 100.0 μl of CAPS pH 10 (1 M) 100.0 μl of di-Sodium hydrogen phosphate (5 M) 66.7 μl of Lithium sulfate (3 M) 250.0 μl of Potassium di-hydrogen phosphate (2 M) 483.3 μl of water (100 v/v) |
D2 |
| pH 8.5 | 600.0 μl of di-Sodium tartrate (2 M) 50.0 μl of Dithiothreitol (0.1 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 250.0 μl of water (100 v/v) |
D3 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 500.0 μl of Potassium Sodium tartrate (2 M) 400.0 μl of water (100 v/v) |
D4 |
| pH 6.5 | 50.0 μl of Cesium chloride (1 M) 600.0 μl of Jeffamine® M-600 (50 v/v) 100.0 μl of MES pH 6.5 (1 M) 250.0 μl of water (100 v/v) |
D5 |
| pH 6.5 | 400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of MES pH 6.5 (1 M) 500.0 μl of water (100 v/v) |
D6 |
| pH 7.5 | 500.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of water (100 v/v) |