C6 - Kinase 1 - Screen Contents

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Supplemental Information

Screen Description

Kinase / Kinase 1

Created 2009-11-16.

Rigaku Recipe XML

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Screen Structure

Well Count	24
Number of distinct ingredients	31
Internal diversity (score mean dist)	0.917

Screen Composition

More information about screen composition chart.

ingredient type	Kinase 1
Buffer	32
Cryoprotectant	2
Organic (non-volatile)	5
Polymer	3
Salt	40

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Kinase 1 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Magnesium acetate 0.2 M - Dithiothreitol 0.01 M - Ammonium sulfate 1 M - tri-Sodium citrate 0.1 M pH 5.6 🔍	pH 5.6	333.3 μl of Ammonium sulfate (3 M) 100.0 μl of Dithiothreitol (0.1 M) 100.0 μl of Magnesium acetate (2 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 366.7 μl of water (100 v/v)
A2	tri-Sodium citrate 0.1 M pH 5.6 Ammonium sulfate 1.3 M - 🔍	pH 5.6	433.3 μl of Ammonium sulfate (3 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 466.7 μl of water (100 v/v)
A3	tris chloride BCC 0.1 M pH 8.5 Ammonium sulfate 1.3 M - 🔍	pH 8.5	433.3 μl of Ammonium sulfate (3 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 466.7 μl of water (100 v/v)
A4	Cobalt (II) chloride 0.025 M - MES 0.1 M pH 6.5 Ammonium sulfate 1.8 M - 🔍	pH 6.5	600.0 μl of Ammonium sulfate (3 M) 25.0 μl of Cobalt (II) chloride (1 M) 100.0 μl of MES pH 6.5 (1 M) 275.0 μl of water (100 v/v)
A5	Sodium chloride 0.2 M - tri-Sodium citrate 0.1 M pH 3.1 Ammonium sulfate 2 M - 🔍	pH 3.1	666.7 μl of Ammonium sulfate (3 M) 200.0 μl of Sodium chloride (1 M) 100.0 μl of tri-Sodium citrate pH 3.1 (1 M) 33.3 μl of water (100 v/v)
A6	Magnesium chloride 0.05 M - Sodium acetate 0.1 M pH 4.6 Ammonium sulfate 2 M - 🔍	pH 4.6	666.7 μl of Ammonium sulfate (3 M) 20.0 μl of Magnesium chloride (2.5 M) 100.0 μl of Sodium acetate pH 4.6 (1 M) 213.3 μl of water (100 v/v)
B1	Sodium acetate 0.1 M pH 4.6 Ammonium sulfate 2 M - 🔍	pH 4.6	666.7 μl of Ammonium sulfate (3 M) 100.0 μl of Sodium acetate pH 4.6 (1 M) 233.3 μl of water (100 v/v)
B2	Polyethylene glycol monomethyl ether 550 2 v/v - HEPES 0.1 M pH 7.5 Ammonium sulfate 2 M - 🔍	pH 7.5	666.7 μl of Ammonium sulfate (3 M) 100.0 μl of HEPES pH 7.5 (1 M) 40.0 μl of Polyethylene glycol monomethyl ether 550 (50 v/v) 193.3 μl of water (100 v/v)
B3	HEPES 0.1 M pH 7.5 Ammonium sulfate 2 M - 🔍	pH 7.5	666.7 μl of Ammonium sulfate (3 M) 100.0 μl of HEPES pH 7.5 (1 M) 233.3 μl of water (100 v/v)
B4	Magnesium chloride 0.006 M - tris chloride BCC 0.1 M pH 8.5 Ammonium sulfate 2 M - 🔍	pH 8.5	666.7 μl of Ammonium sulfate (3 M) 2.4 μl of Magnesium chloride (2.5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 230.9 μl of water (100 v/v)
B5	Nickel sulfate 0.01 M - tris chloride BCC 0.1 M pH 8.5 Lithium sulfate 1.5 M - 🔍	pH 8.5	500.0 μl of Lithium sulfate (3 M) 2.5 μl of Nickel sulfate (4 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 397.5 μl of water (100 v/v)
B6	tri-Sodium citrate 0.1 M pH 4.2 Lithium chloride 1 M - 🔍	pH 4.2	200.0 μl of Lithium chloride (5 M) 100.0 μl of tri-Sodium citrate pH 4.2 (1 M) 700.0 μl of water (100 v/v)
C1	Sodium acetate 0.1 M pH 4.6 Sodium chloride 2 M - 🔍	pH 4.6	100.0 μl of Sodium acetate pH 4.6 (1 M) 400.0 μl of Sodium chloride (5 M) 500.0 μl of water (100 v/v)
C2	di-Sodium hydrogen phosphate 0.1 M - MES 0.1 M pH 6.5 Sodium chloride 2 M - Potassium di-hydrogen phosphate 0.1 M - 🔍	pH 6.5	20.0 μl of di-Sodium hydrogen phosphate (5 M) 100.0 μl of MES pH 6.5 (1 M) 50.0 μl of Potassium di-hydrogen phosphate (2 M) 400.0 μl of Sodium chloride (5 M) 430.0 μl of water (100 v/v)
C3	Glycerol 1 v/v - HEPES 0.1 M pH 7.5 Sodium chloride 3.3 M - 🔍	pH 7.5	10.0 μl of Glycerol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 660.0 μl of Sodium chloride (5 M) 230.0 μl of water (100 v/v)
C4	Calcium chloride 0.00625 M - MES 0.1 M pH 6.5 Sodium acetate 1.2 M - 🔍	pH 6.5	6.2 μl of Calcium chloride (1 M) 100.0 μl of MES pH 6.5 (1 M) 600.0 μl of Sodium acetate (2 M) 293.8 μl of water (100 v/v)
C5	Polyethylene glycol 3,000 2 w/v - BICINE 0.1 M pH 9.5 Sodium formate 3.7 M - 🔍	pH 9.5	100.0 μl of BICINE pH 9.5 (1 M) 40.0 μl of Polyethylene glycol 3,000 (50 w/v) 528.6 μl of Sodium formate (7 M) 331.4 μl of water (100 v/v)
C6	Glycerol 1.6 v/v - Dithiothreitol 0.01 M - di-Sodium malonate 0.5 M - PIPES 0.05 M pH 6 🔍	pH 6	166.7 μl of di-Sodium malonate pH 6 (3 M) 100.0 μl of Dithiothreitol (0.1 M) 16.0 μl of Glycerol (100 v/v) 50.0 μl of PIPES pH 6 (1 M) 667.3 μl of water (100 v/v)
D1	Potassium di-hydrogen phosphate 0.5 M - Lithium sulfate 0.2 M - di-Sodium hydrogen phosphate 0.5 M - CAPS 0.1 M pH 10 🔍	pH 10	100.0 μl of CAPS pH 10 (1 M) 100.0 μl of di-Sodium hydrogen phosphate (5 M) 66.7 μl of Lithium sulfate (3 M) 250.0 μl of Potassium di-hydrogen phosphate (2 M) 483.3 μl of water (100 v/v)
D2	Dithiothreitol 0.005 M - tris chloride BCC 0.1 M pH 8.5 di-Sodium tartrate 1.2 M - 🔍	pH 8.5	600.0 μl of di-Sodium tartrate (2 M) 50.0 μl of Dithiothreitol (0.1 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 250.0 μl of water (100 v/v)
D3	MES 0.1 M pH 6.5 Potassium Sodium tartrate 1 M - 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 500.0 μl of Potassium Sodium tartrate (2 M) 400.0 μl of water (100 v/v)
D4	Cesium chloride 0.05 M - MES 0.1 M pH 6.5 Jeffamine® M-600 30 v/v - 🔍	pH 6.5	50.0 μl of Cesium chloride (1 M) 600.0 μl of Jeffamine® M-600 (50 v/v) 100.0 μl of MES pH 6.5 (1 M) 250.0 μl of water (100 v/v)
D5	MES 0.1 M pH 6.5 2-methyl-2,4-pentanediol BCC 40 v/v - 🔍	pH 6.5	400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of MES pH 6.5 (1 M) 500.0 μl of water (100 v/v)
D6	HEPES 0.1 M pH 7.5 2-methyl-2,4-pentanediol BCC 50 v/v - 🔍	pH 7.5	500.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of water (100 v/v)