C6 - Screen Contents

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Supplemental Information

Screen Description

Kinase 1

Kinase / Kinase 1

Created 2009-11-16.

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Kinase 1

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Kinase 1

Screen Structure

Kinase 1

Well Count	24
Number of distinct ingredients	31
Internal diversity (score mean dist)	0.917

Screen Composition

More information about screen composition chart.

ingredient type	Kinase 1
Buffer	32
Cryoprotectant	2
Organic (non-volatile)	5
Polymer	3
Salt	40

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Kinase 1 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Magnesium acetate 0.2 M - Dithiothreitol 0.01 M - Ammonium sulfate 1 M - tri-Sodium citrate 0.1 M pH 5.6 🔍	pH 5.6	100 μl of tri-Sodium citrate pH 5.6 (1 M) 100 μl of Magnesium acetate (2 M) 333 μl of Ammonium sulfate (3 M) 100 μl of Dithiothreitol (0.1 M) 366 μl of water (100 v/v)
A2	tri-Sodium citrate 0.1 M pH 5.6 Ammonium sulfate 1.3 M - 🔍	pH 5.6	100 μl of tri-Sodium citrate pH 5.6 (1 M) 433 μl of Ammonium sulfate (3 M) 466 μl of water (100 v/v)
A3	tris chloride BCC 0.1 M pH 8.5 Ammonium sulfate 1.3 M - 🔍	pH 8.5	100 μl of tris chloride BCC pH 8.5 (1 M) 433 μl of Ammonium sulfate (3 M) 466 μl of water (100 v/v)
A4	Cobalt (II) chloride 0.025 M - MES 0.1 M pH 6.5 Ammonium sulfate 1.8 M - 🔍	pH 6.5	100 μl of MES pH 6.5 (1 M) 25 μl of Cobalt (II) chloride (1 M) 600 μl of Ammonium sulfate (3 M) 275 μl of water (100 v/v)
A5	Sodium chloride 0.2 M - tri-Sodium citrate 0.1 M pH 3.1 Ammonium sulfate 2 M - 🔍	pH 3.1	100 μl of tri-Sodium citrate pH 3.1 (1 M) 200 μl of Sodium chloride (1 M) 666 μl of Ammonium sulfate (3 M) 33 μl of water (100 v/v)
A6	Magnesium chloride 0.05 M - Sodium acetate 0.1 M pH 4.6 Ammonium sulfate 2 M - 🔍	pH 4.6	100 μl of Sodium acetate pH 4.6 (1 M) 20 μl of Magnesium chloride (2.5 M) 666 μl of Ammonium sulfate (3 M) 213 μl of water (100 v/v)
B1	Sodium acetate 0.1 M pH 4.6 Ammonium sulfate 2 M - 🔍	pH 4.6	100 μl of Sodium acetate pH 4.6 (1 M) 666 μl of Ammonium sulfate (3 M) 233 μl of water (100 v/v)
B2	Polyethylene glycol monomethyl ether 550 2 v/v - HEPES 0.1 M pH 7.5 Ammonium sulfate 2 M - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 40 μl of Polyethylene glycol monomethyl ether 550 (50 v/v) 666 μl of Ammonium sulfate (3 M) 193 μl of water (100 v/v)
B3	HEPES 0.1 M pH 7.5 Ammonium sulfate 2 M - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 666 μl of Ammonium sulfate (3 M) 233 μl of water (100 v/v)
B4	Magnesium chloride 0.006 M - tris chloride BCC 0.1 M pH 8.5 Ammonium sulfate 2 M - 🔍	pH 8.5	2 μl of Magnesium chloride (2.5 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 666 μl of Ammonium sulfate (3 M) 230 μl of water (100 v/v)
B5	Nickel sulfate 0.01 M - tris chloride BCC 0.1 M pH 8.5 Lithium sulfate 1.5 M - 🔍	pH 8.5	2 μl of Nickel sulfate (4 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 500 μl of Lithium sulfate (3 M) 397 μl of water (100 v/v)
B6	tri-Sodium citrate 0.1 M pH 4.2 Lithium chloride 1 M - 🔍	pH 4.2	200 μl of Lithium chloride (5 M) 100 μl of tri-Sodium citrate pH 4.2 (1 M) 700 μl of water (100 v/v)
C1	Sodium acetate 0.1 M pH 4.6 Sodium chloride 2 M - 🔍	pH 4.6	100 μl of Sodium acetate pH 4.6 (1 M) 400 μl of Sodium chloride (5 M) 500 μl of water (100 v/v)
C2	di-Sodium hydrogen phosphate 0.1 M - MES 0.1 M pH 6.5 Sodium chloride 2 M - Potassium di-hydrogen phosphate 0.1 M - 🔍	pH 6.5	50 μl of Potassium di-hydrogen phosphate (2 M) 20 μl of di-Sodium hydrogen phosphate (5 M) 400 μl of Sodium chloride (5 M) 100 μl of MES pH 6.5 (1 M) 430 μl of water (100 v/v)
C3	Glycerol 1 v/v - HEPES 0.1 M pH 7.5 Sodium chloride 3.3 M - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 10 μl of Glycerol (100 v/v) 660 μl of Sodium chloride (5 M) 230 μl of water (100 v/v)
C4	Calcium chloride 0.00625 M - MES 0.1 M pH 6.5 Sodium acetate 1.2 M - 🔍	pH 6.5	600 μl of Sodium acetate (2 M) 100 μl of MES pH 6.5 (1 M) 6 μl of Calcium chloride (1 M) 293 μl of water (100 v/v)
C5	Polyethylene glycol 3,000 2 w/v - BICINE 0.1 M pH 9.5 Sodium formate 3.7 M - 🔍	pH 9.5	40 μl of Polyethylene glycol 3,000 (50 w/v) 528 μl of Sodium formate (7 M) 100 μl of BICINE pH 9.5 (1 M) 331 μl of water (100 v/v)
C6	Glycerol 1.6 v/v - Dithiothreitol 0.01 M - di-Sodium malonate 0.5 M - PIPES 0.05 M pH 6 🔍	pH 6	166 μl of di-Sodium malonate pH 6 (3 M) 16 μl of Glycerol (100 v/v) 50 μl of PIPES pH 6 (1 M) 100 μl of Dithiothreitol (0.1 M) 667 μl of water (100 v/v)
D1	Potassium di-hydrogen phosphate 0.5 M - Lithium sulfate 0.2 M - di-Sodium hydrogen phosphate 0.5 M - CAPS 0.1 M pH 10 🔍	pH 10	100 μl of CAPS pH 10 (1 M) 250 μl of Potassium di-hydrogen phosphate (2 M) 100 μl of di-Sodium hydrogen phosphate (5 M) 66 μl of Lithium sulfate (3 M) 483 μl of water (100 v/v)
D2	Dithiothreitol 0.005 M - tris chloride BCC 0.1 M pH 8.5 di-Sodium tartrate 1.2 M - 🔍	pH 8.5	600 μl of di-Sodium tartrate (2 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 50 μl of Dithiothreitol (0.1 M) 250 μl of water (100 v/v)
D3	MES 0.1 M pH 6.5 Potassium Sodium tartrate 1 M - 🔍	pH 6.5	500 μl of Potassium Sodium tartrate (2 M) 100 μl of MES pH 6.5 (1 M) 400 μl of water (100 v/v)
D4	Cesium chloride 0.05 M - MES 0.1 M pH 6.5 Jeffamine® M-600 30 v/v - 🔍	pH 6.5	600 μl of Jeffamine® M-600 (50 v/v) 50 μl of Cesium chloride (1 M) 100 μl of MES pH 6.5 (1 M) 250 μl of water (100 v/v)
D5	MES 0.1 M pH 6.5 2-methyl-2,4-pentanediol BCC 40 v/v - 🔍	pH 6.5	400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100 μl of MES pH 6.5 (1 M) 500 μl of water (100 v/v)
D6	HEPES 0.1 M pH 7.5 2-methyl-2,4-pentanediol BCC 50 v/v - 🔍	pH 7.5	500 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100 μl of HEPES pH 7.5 (1 M) 400 μl of water (100 v/v)