C6 - Screen Contents

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Supplemental Information

Screen Description

Kinase 2

Kinase / Kinase 2

Created 2009-11-16.

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Kinase 2

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Kinase 2

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Screen Structure

Kinase 2

Well Count	24
Number of distinct ingredients	32
Internal diversity (score mean dist)	0.923

Screen Composition

More information about screen composition chart.

ingredient type	Kinase 2
Buffer	26
Organic (non-volatile)	6
Organic (volatile)	2
Polymer	24
Salt	30

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Kinase 2 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Dithiothreitol 0.001 M - Ethylenediaminetetraacetic acid disodium salt 1 mM - Polyethylene glycol 400 10 v/v - tris chloride BCC 0.05 M pH 8.5 Sodium chloride 0.3 M - 🔍	pH 8.5	10.0 μl of Dithiothreitol (0.1 M) 142.9 μl of Ethylenediaminetetraacetic acid disodium salt (7 mM) 100.0 μl of Polyethylene glycol 400 (100 v/v) 300.0 μl of Sodium chloride (1 M) 50.0 μl of tris chloride BCC pH 8.5 (1 M) 397.1 μl of water (100 v/v)
A2	Calcium chloride 0.2 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 400 15 v/v - 🔍	pH 7.5	200.0 μl of Calcium chloride (1 M) 100.0 μl of HEPES pH 7.5 (1 M) 150.0 μl of Polyethylene glycol 400 (100 v/v) 550.0 μl of water (100 v/v)
A3	2-Propanol 10 v/v - MES 0.1 M pH 6.5 Polyethylene glycol 400 25 v/v - 🔍	pH 6.5	100.0 μl of 2-Propanol (100 v/v) 100.0 μl of MES pH 6.5 (1 M) 250.0 μl of Polyethylene glycol 400 (100 v/v) 550.0 μl of water (100 v/v)
A4	tri-Sodium citrate 0.15 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 400 25 v/v - 🔍	pH 8.5	250.0 μl of Polyethylene glycol 400 (100 v/v) 75.0 μl of tri-Sodium citrate (2 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 575.0 μl of water (100 v/v)
A5	Ethylene glycol 5 v/v - Sodium acetate 0.1 M pH 4.6 Polyethylene glycol monomethyl ether 550 15 v/v - 🔍	pH 4.6	100.0 μl of Ethylene glycol (50 v/v) 150.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 650.0 μl of water (100 v/v)
A6	Sodium chloride 0.1 M - BICINE 0.1 M pH 9 Polyethylene glycol monomethyl ether 550 20 v/v - 🔍	pH 9	100.0 μl of BICINE pH 9 (1 M) 200.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 100.0 μl of Sodium chloride (1 M) 600.0 μl of water (100 v/v)
B1	Dithiothreitol 0.001 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 1,000 20 w/v - 🔍	pH 8.5	10.0 μl of Dithiothreitol (0.1 M) 400.0 μl of Polyethylene glycol 1,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 490.0 μl of water (100 v/v)
B2	Lithium sulfate 0.05 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 1,000 35 w/v - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 16.7 μl of Lithium sulfate (3 M) 700.0 μl of Polyethylene glycol 1,000 (50 w/v) 183.3 μl of water (100 v/v)
B3	Magnesium acetate 0.2 M - MES 0.1 M pH 6.5 Polyethylene glycol 2,000 12 w/v - 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 100.0 μl of Magnesium acetate (2 M) 240.0 μl of Polyethylene glycol 2,000 (50 w/v) 560.0 μl of water (100 v/v)
B4	Magnesium chloride 0.1 M - Sodium acetate 0.1 M pH 4.6 Polyethylene glycol 2,000 25 w/v - 🔍	pH 4.6	40.0 μl of Magnesium chloride (2.5 M) 500.0 μl of Polyethylene glycol 2,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 360.0 μl of water (100 v/v)
B5	Magnesium chloride 0.05 M - Sodium acetate 0.1 M pH 4.6 Polyethylene glycol 2,000 30 w/v - 🔍	pH 4.6	20.0 μl of Magnesium chloride (2.5 M) 600.0 μl of Polyethylene glycol 2,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 280.0 μl of water (100 v/v)
B6	Sodium phosphate citrate 0.1 M pH 5 Polyethylene glycol monomethyl ether 2,000 24 w/v - 🔍	pH 5	480.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100.0 μl of Sodium phosphate citrate pH 5 (1 M) 420.0 μl of water (100 v/v)
C1	Sodium chloride 0.5 M - MES 0.1 M pH 6.5 Polyethylene glycol 3,350 12 w/v - 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 240.0 μl of Polyethylene glycol 3,350 (50 w/v) 500.0 μl of Sodium chloride (1 M) 160.0 μl of water (100 v/v)
C2	Ammonium sulfate 0.2 M - Magnesium sulfate 0.05 M - Polyethylene glycol 3,350 12 w/v - tri-Sodium citrate 0.05 M pH 5.6 🔍	pH 5.6	66.7 μl of Ammonium sulfate (3 M) 50.0 μl of Magnesium sulfate (1 M) 240.0 μl of Polyethylene glycol 3,350 (50 w/v) 50.0 μl of tri-Sodium citrate pH 5.6 (1 M) 593.3 μl of water (100 v/v)
C3	Ammonium sulfate 0.25 M - Cadmium chloride 0.01 M - Polyethylene glycol 3,350 15 w/v - Imidazole 0.1 M pH 7.5 🔍	pH 7.5	83.3 μl of Ammonium sulfate (3 M) 10.0 μl of Cadmium chloride (1 M) 100.0 μl of Imidazole pH 7.5 (1 M) 300.0 μl of Polyethylene glycol 3,350 (50 w/v) 506.7 μl of water (100 v/v)
C4	di-Sodium DL-malate 0.15 M pH 7 Polyethylene glycol 3,350 20 w/v - 🔍	pH 7	400.0 μl of Polyethylene glycol 3,350 (50 w/v) 50.0 μl of di-Sodium DL-malate pH 7 (3 M) 550.0 μl of water (100 v/v)
C5	Sodium chloride 0.2 M - L-Glutathione reduced 20 mM - Polyethylene glycol 3,350 20 w/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 100.0 μl of L-Glutathione reduced (200 mM) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 200.0 μl of Sodium chloride (1 M) 200.0 μl of water (100 v/v)
C6	Sodium chloride 0.12 M - Dithiothreitol 0.005 M - Polyethylene glycol 3,350 20 w/v - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	50.0 μl of Dithiothreitol (0.1 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 120.0 μl of Sodium chloride (1 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 330.0 μl of water (100 v/v)
D1	Potassium nitrate 0.2 M - Polyethylene glycol 3,350 20 w/v - 🔍	NA	400.0 μl of Polyethylene glycol 3,350 (50 w/v) 200.0 μl of Potassium nitrate (1 M) 400.0 μl of water (100 v/v)
D2	Ammonium formate 0.1 M - Polyethylene glycol 3,350 22 w/v - 🔍	NA	40.0 μl of Ammonium formate (2.5 M) 440.0 μl of Polyethylene glycol 3,350 (50 w/v) 520.0 μl of water (100 v/v)
D3	tri-Sodium citrate 0.1 M pH 5 Polyethylene glycol 3,350 24 w/v - 🔍	pH 5	480.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 420.0 μl of water (100 v/v)
D4	Ammonium acetate 0.2 M - Polyethylene glycol 3,350 30 w/v - Sodium acetate 0.1 M pH 4.6 🔍	pH 4.6	40.0 μl of Ammonium acetate (5 M) 600.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 260.0 μl of water (100 v/v)
D5	2-Propanol 20 v/v - Calcium chloride 0.2 M - Polyethylene glycol 3,350 30 w/v - Ammonium acetate 0.2 M pH 5.6 🔍	pH 5.6	200.0 μl of 2-Propanol (100 v/v) 200.0 μl of Ammonium acetate pH 5.6 (1 M) 40.0 μl of Calcium chloride (5 M) 375.0 μl of Polyethylene glycol 3,350 (80 w/v) 185.0 μl of water (100 v/v)
D6	Sodium chloride 0.5 M - Magnesium chloride 0.2 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 3,350 32.5 w/v - 🔍	pH 8.5	80.0 μl of Magnesium chloride (2.5 M) 650.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of Sodium chloride (5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 70.0 μl of water (100 v/v)