C6 - Screen Contents

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Supplemental Information

Screen Description

Kinase 2

Kinase / Kinase 2

Created 2009-11-16.

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Kinase 2

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Kinase 2

Screen Structure

Kinase 2

Well Count	24
Number of distinct ingredients	32
Internal diversity (score mean dist)	0.923

Screen Composition

More information about screen composition chart.

ingredient type	Kinase 2
Buffer	26
Organic (non-volatile)	6
Organic (volatile)	2
Polymer	24
Salt	30

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Kinase 2 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Dithiothreitol 0.001 M - Ethylenediaminetetraacetic acid disodium salt 1 mM - Polyethylene glycol 400 10 v/v - tris chloride BCC 0.05 M pH 8.5 Sodium chloride 0.3 M - 🔍	pH 8.5	142 μl of Ethylenediaminetetraacetic acid disodium salt (7 mM) 300 μl of Sodium chloride (1 M) 50 μl of tris chloride BCC pH 8.5 (1 M) 100 μl of Polyethylene glycol 400 (100 v/v) 10 μl of Dithiothreitol (0.1 M) 397 μl of water (100 v/v)
A2	Calcium chloride 0.2 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 400 15 v/v - 🔍	pH 7.5	200 μl of Calcium chloride (1 M) 100 μl of HEPES pH 7.5 (1 M) 150 μl of Polyethylene glycol 400 (100 v/v) 550 μl of water (100 v/v)
A3	2-Propanol 10 v/v - MES 0.1 M pH 6.5 Polyethylene glycol 400 25 v/v - 🔍	pH 6.5	100 μl of MES pH 6.5 (1 M) 250 μl of Polyethylene glycol 400 (100 v/v) 100 μl of 2-Propanol (100 v/v) 550 μl of water (100 v/v)
A4	tri-Sodium citrate 0.15 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 400 25 v/v - 🔍	pH 8.5	100 μl of tris chloride BCC pH 8.5 (1 M) 75 μl of tri-Sodium citrate (2 M) 250 μl of Polyethylene glycol 400 (100 v/v) 575 μl of water (100 v/v)
A5	Ethylene glycol 5 v/v - Sodium acetate 0.1 M pH 4.6 Polyethylene glycol monomethyl ether 550 15 v/v - 🔍	pH 4.6	150 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 100 μl of Ethylene glycol (50 v/v) 100 μl of Sodium acetate pH 4.6 (1 M) 650 μl of water (100 v/v)
A6	Sodium chloride 0.1 M - BICINE 0.1 M pH 9 Polyethylene glycol monomethyl ether 550 20 v/v - 🔍	pH 9	100 μl of Sodium chloride (1 M) 100 μl of BICINE pH 9 (1 M) 200 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 600 μl of water (100 v/v)
B1	Dithiothreitol 0.001 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 1,000 20 w/v - 🔍	pH 8.5	100 μl of tris chloride BCC pH 8.5 (1 M) 10 μl of Dithiothreitol (0.1 M) 400 μl of Polyethylene glycol 1,000 (50 w/v) 490 μl of water (100 v/v)
B2	Lithium sulfate 0.05 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 1,000 35 w/v - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 16 μl of Lithium sulfate (3 M) 700 μl of Polyethylene glycol 1,000 (50 w/v) 183 μl of water (100 v/v)
B3	Magnesium acetate 0.2 M - MES 0.1 M pH 6.5 Polyethylene glycol 2,000 12 w/v - 🔍	pH 6.5	100 μl of MES pH 6.5 (1 M) 240 μl of Polyethylene glycol 2,000 (50 w/v) 100 μl of Magnesium acetate (2 M) 560 μl of water (100 v/v)
B4	Magnesium chloride 0.1 M - Sodium acetate 0.1 M pH 4.6 Polyethylene glycol 2,000 25 w/v - 🔍	pH 4.6	500 μl of Polyethylene glycol 2,000 (50 w/v) 40 μl of Magnesium chloride (2.5 M) 100 μl of Sodium acetate pH 4.6 (1 M) 360 μl of water (100 v/v)
B5	Magnesium chloride 0.05 M - Sodium acetate 0.1 M pH 4.6 Polyethylene glycol 2,000 30 w/v - 🔍	pH 4.6	600 μl of Polyethylene glycol 2,000 (50 w/v) 20 μl of Magnesium chloride (2.5 M) 100 μl of Sodium acetate pH 4.6 (1 M) 280 μl of water (100 v/v)
B6	Sodium phosphate citrate 0.1 M pH 5 Polyethylene glycol monomethyl ether 2,000 24 w/v - 🔍	pH 5	100 μl of Sodium phosphate citrate pH 5 (1 M) 480 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 420 μl of water (100 v/v)
C1	Sodium chloride 0.5 M - MES 0.1 M pH 6.5 Polyethylene glycol 3,350 12 w/v - 🔍	pH 6.5	500 μl of Sodium chloride (1 M) 240 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of MES pH 6.5 (1 M) 160 μl of water (100 v/v)
C2	Ammonium sulfate 0.2 M - Magnesium sulfate 0.05 M - Polyethylene glycol 3,350 12 w/v - tri-Sodium citrate 0.05 M pH 5.6 🔍	pH 5.6	240 μl of Polyethylene glycol 3,350 (50 w/v) 50 μl of tri-Sodium citrate pH 5.6 (1 M) 50 μl of Magnesium sulfate (1 M) 66 μl of Ammonium sulfate (3 M) 593 μl of water (100 v/v)
C3	Ammonium sulfate 0.25 M - Cadmium chloride 0.01 M - Polyethylene glycol 3,350 15 w/v - Imidazole 0.1 M pH 7.5 🔍	pH 7.5	300 μl of Polyethylene glycol 3,350 (50 w/v) 10 μl of Cadmium chloride (1 M) 83 μl of Ammonium sulfate (3 M) 100 μl of Imidazole pH 7.5 (1 M) 506 μl of water (100 v/v)
C4	di-Sodium DL-malate 0.15 M pH 7 Polyethylene glycol 3,350 20 w/v - 🔍	pH 7	400 μl of Polyethylene glycol 3,350 (50 w/v) 50 μl of di-Sodium DL-malate pH 7 (3 M) 550 μl of water (100 v/v)
C5	Sodium chloride 0.2 M - L-Glutathione reduced 20 mM - Polyethylene glycol 3,350 20 w/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	200 μl of Sodium chloride (1 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of L-Glutathione reduced (200 mM) 100 μl of HEPES pH 7.5 (1 M) 200 μl of water (100 v/v)
C6	Sodium chloride 0.12 M - Dithiothreitol 0.005 M - Polyethylene glycol 3,350 20 w/v - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	120 μl of Sodium chloride (1 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 50 μl of Dithiothreitol (0.1 M) 330 μl of water (100 v/v)
D1	Potassium nitrate 0.2 M - Polyethylene glycol 3,350 20 w/v - 🔍	NA	400 μl of Polyethylene glycol 3,350 (50 w/v) 200 μl of Potassium nitrate (1 M) 400 μl of water (100 v/v)
D2	Ammonium formate 0.1 M - Polyethylene glycol 3,350 22 w/v - 🔍	NA	440 μl of Polyethylene glycol 3,350 (50 w/v) 40 μl of Ammonium formate (2.5 M) 520 μl of water (100 v/v)
D3	tri-Sodium citrate 0.1 M pH 5 Polyethylene glycol 3,350 24 w/v - 🔍	pH 5	480 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of tri-Sodium citrate pH 5 (1 M) 420 μl of water (100 v/v)
D4	Ammonium acetate 0.2 M - Polyethylene glycol 3,350 30 w/v - Sodium acetate 0.1 M pH 4.6 🔍	pH 4.6	600 μl of Polyethylene glycol 3,350 (50 w/v) 40 μl of Ammonium acetate (5 M) 100 μl of Sodium acetate pH 4.6 (1 M) 260 μl of water (100 v/v)
D5	2-Propanol 20 v/v - Calcium chloride 0.2 M - Polyethylene glycol 3,350 30 w/v - Ammonium acetate 0.2 M pH 5.6 🔍	pH 5.6	200 μl of Ammonium acetate pH 5.6 (1 M) 40 μl of Calcium chloride (5 M) 200 μl of 2-Propanol (100 v/v) 375 μl of Polyethylene glycol 3,350 (80 w/v) 185 μl of water (100 v/v)
D6	Sodium chloride 0.5 M - Magnesium chloride 0.2 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 3,350 32.5 w/v - 🔍	pH 8.5	650 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 100 μl of Sodium chloride (5 M) 80 μl of Magnesium chloride (2.5 M) 70 μl of water (100 v/v)