Welcome to the new C6 website. This website aims preserve as many features as possible of the original C6 website.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
Kinase / Kinase 3
Created 2009-11-16.
Rigaku Recipe XML
Export
Barcodes
No barcodes found.
Screen Structure
Well Count | 24 |
---|---|
Number of distinct ingredients | 19 |
Internal diversity (score mean dist) | 0.860 |
Screen Composition
ingredient type | Kinase 3 |
---|---|
Buffer | 26 |
Organic (non-volatile) | 1 |
Organic (volatile) | 3 |
Polymer | 25 |
Salt | 18 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 6.5 | 100.0 μl of Dithiothreitol (0.1 M) 4.0 μl of Magnesium chloride (2.5 M) 50.0 μl of MES pH 6.5 (1 M) 160.0 μl of Polyethylene glycol 4,000 (50 w/v) 686.0 μl of water (100 v/v) |
A2 |
| pH 7 | 100.0 μl of Dithiothreitol (0.1 M) 50.0 μl of PIPES pH 7 (1 M) 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 650.0 μl of water (100 v/v) |
A3 |
| pH 7.5 | 100.0 μl of 2-Propanol (100 v/v) 150.0 μl of Ethylene glycol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 80.0 μl of Magnesium chloride (2.5 M) 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 370.0 μl of water (100 v/v) |
A4 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 700.0 μl of water (100 v/v) |
A5 |
| pH 7.5 | 100.0 μl of 2-Propanol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 300.0 μl of Polyethylene glycol 4,000 (50 w/v) 500.0 μl of water (100 v/v) |
A6 |
| pH 8.5 | 300.0 μl of Polyethylene glycol 4,000 (50 w/v) 20.0 μl of Polyethylene glycol 6,000 (50 w/v) 37.5 μl of Sodium acetate (2 M) 200.0 μl of Sodium chloride (1 M) 75.0 μl of tris chloride BCC pH 8.5 (1 M) 367.5 μl of water (100 v/v) |
B1 |
| pH 5.5 | 66.7 μl of Ammonium sulfate (3 M) 11.5 μl of di-Sodium DL-malate pH 5 (3 M) 21.9 μl of di-Sodium DL-malate pH 7 (3 M) 300.0 μl of Polyethylene glycol 4,000 (50 w/v) 600.0 μl of water (100 v/v) |
B2 |
| pH 6.5 | 100.0 μl of BIS-TRIS pH 6.5 (1 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium chloride (1 M) 400.0 μl of water (100 v/v) |
B3 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 80.0 μl of Magnesium chloride (2.5 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 420.0 μl of water (100 v/v) |
B4 |
| pH 8.5 | 80.0 μl of Magnesium chloride (2.5 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 420.0 μl of water (100 v/v) |
B5 |
| pH 6.5 | 80.0 μl of Magnesium chloride (2.5 M) 100.0 μl of MES pH 6.5 (1 M) 500.0 μl of Polyethylene glycol 4,000 (50 w/v) 320.0 μl of water (100 v/v) |
B6 |
| pH 8.5 | 33.3 μl of Lithium sulfate (3 M) 500.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 366.7 μl of water (100 v/v) |
C1 |
| pH 7.5 | 200.0 μl of Lithium acetate pH 7.5 (1 M) 560.0 μl of Polyethylene glycol 4,000 (50 w/v) 240.0 μl of water (100 v/v) |
C2 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium acetate (2 M) 200.0 μl of water (100 v/v) |
C3 |
| pH 8.5 | 66.7 μl of Ammonium sulfate (3 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 150.0 μl of tris chloride BCC pH 8.5 (1 M) 183.3 μl of water (100 v/v) |
C4 |
| pH 7.5 | 100.0 μl of 2-Propanol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 160.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 640.0 μl of water (100 v/v) |
C5 |
| pH 6.5 | 66.7 μl of Ammonium sulfate (3 M) 100.0 μl of MES pH 6.5 (1 M) 500.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 333.3 μl of water (100 v/v) |
C6 |
| pH 7.5 | 66.7 μl of Ammonium sulfate (3 M) 100.0 μl of HEPES pH 7.5 (1 M) 600.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 233.3 μl of water (100 v/v) |
D1 |
| pH 6.5 | 100.0 μl of ADA pH 6.5 (1 M) 33.3 μl of Ammonium sulfate (3 M) 600.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 266.7 μl of water (100 v/v) |
D2 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 500.0 μl of water (100 v/v) |
D3 |
| pH 6.5 | 100.0 μl of Dithiothreitol (0.1 M) 100.0 μl of MES pH 6.5 (1 M) 560.0 μl of Polyethylene glycol 6,000 (50 w/v) 240.0 μl of water (100 v/v) |
D4 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 58.3 μl of Lithium sulfate (3 M) 600.0 μl of Polyethylene glycol 6,000 (50 w/v) 241.7 μl of water (100 v/v) |
D5 |
| pH 7 | 100.0 μl of Dithiothreitol (0.1 M) 100.0 μl of PIPES pH 7 (1 M) 600.0 μl of Polyethylene glycol 6,000 (50 w/v) 200.0 μl of water (100 v/v) |
D6 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 640.0 μl of Polyethylene glycol 6,000 (50 w/v) 260.0 μl of water (100 v/v) |