C6 - Kinase 3 - Screen Contents

Welcome to the new C6 website. This website aims preserve as many features as possible of the original C6 website.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.

Jump to well contents.

Supplemental Information

Screen Description

Kinase / Kinase 3

Created 2009-11-16.

Rigaku Recipe XML

Export

Barcodes

No barcodes found.

Screen Structure

Well Count	24
Number of distinct ingredients	19
Internal diversity (score mean dist)	0.860

Screen Composition

More information about screen composition chart.

ingredient type	Kinase 3
Buffer	26
Organic (non-volatile)	1
Organic (volatile)	3
Polymer	25
Salt	18

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Kinase 3 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Magnesium chloride 0.01 M - Dithiothreitol 0.01 M - Polyethylene glycol 4,000 8 w/v - MES 0.05 M pH 6.5 🔍	pH 6.5	100.0 μl of Dithiothreitol (0.1 M) 4.0 μl of Magnesium chloride (2.5 M) 50.0 μl of MES pH 6.5 (1 M) 160.0 μl of Polyethylene glycol 4,000 (50 w/v) 686.0 μl of water (100 v/v)
A2	Dithiothreitol 0.01 M - PIPES 0.05 M pH 7 Polyethylene glycol 4,000 10 w/v - 🔍	pH 7	100.0 μl of Dithiothreitol (0.1 M) 50.0 μl of PIPES pH 7 (1 M) 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 650.0 μl of water (100 v/v)
A3	Ethylene glycol 15 v/v - 2-Propanol 10 v/v - Magnesium chloride 0.2 M - Polyethylene glycol 4,000 10 w/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100.0 μl of 2-Propanol (100 v/v) 150.0 μl of Ethylene glycol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 80.0 μl of Magnesium chloride (2.5 M) 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 370.0 μl of water (100 v/v)
A4	HEPES 0.1 M pH 7.5 Polyethylene glycol 4,000 10 w/v - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 700.0 μl of water (100 v/v)
A5	2-Propanol 10 v/v - HEPES 0.1 M pH 7.5 Polyethylene glycol 4,000 15 w/v - 🔍	pH 7.5	100.0 μl of 2-Propanol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 300.0 μl of Polyethylene glycol 4,000 (50 w/v) 500.0 μl of water (100 v/v)
A6	Sodium chloride 0.2 M - Polyethylene glycol 6,000 1 w/v - Sodium acetate 0.075 M - Polyethylene glycol 4,000 15 w/v - tris chloride BCC 0.075 M pH 8.5 🔍	pH 8.5	300.0 μl of Polyethylene glycol 4,000 (50 w/v) 20.0 μl of Polyethylene glycol 6,000 (50 w/v) 37.5 μl of Sodium acetate (2 M) 200.0 μl of Sodium chloride (1 M) 75.0 μl of tris chloride BCC pH 8.5 (1 M) 367.5 μl of water (100 v/v)
B1	Ammonium sulfate 0.2 M - di-Sodium DL-malate 0.1 M pH 5.5 Polyethylene glycol 4,000 15 w/v - 🔍	pH 5.5	66.7 μl of Ammonium sulfate (3 M) 11.5 μl of di-Sodium DL-malate pH 5 (3 M) 21.9 μl of di-Sodium DL-malate pH 7 (3 M) 300.0 μl of Polyethylene glycol 4,000 (50 w/v) 600.0 μl of water (100 v/v)
B2	Sodium chloride 0.1 M - BIS-TRIS 0.1 M pH 6.5 Polyethylene glycol 4,000 20 w/v - 🔍	pH 6.5	100.0 μl of BIS-TRIS pH 6.5 (1 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium chloride (1 M) 400.0 μl of water (100 v/v)
B3	Magnesium chloride 0.2 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 4,000 20 w/v - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 80.0 μl of Magnesium chloride (2.5 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 420.0 μl of water (100 v/v)
B4	Magnesium chloride 0.2 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 4,000 20 w/v - 🔍	pH 8.5	80.0 μl of Magnesium chloride (2.5 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 420.0 μl of water (100 v/v)
B5	Magnesium chloride 0.2 M - MES 0.1 M pH 6.5 Polyethylene glycol 4,000 25 w/v - 🔍	pH 6.5	80.0 μl of Magnesium chloride (2.5 M) 100.0 μl of MES pH 6.5 (1 M) 500.0 μl of Polyethylene glycol 4,000 (50 w/v) 320.0 μl of water (100 v/v)
B6	Lithium sulfate 0.1 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 4,000 25 w/v - 🔍	pH 8.5	33.3 μl of Lithium sulfate (3 M) 500.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 366.7 μl of water (100 v/v)
C1	Lithium acetate 0.2 M pH 7.5 Polyethylene glycol 4,000 28 w/v - 🔍	pH 7.5	200.0 μl of Lithium acetate pH 7.5 (1 M) 560.0 μl of Polyethylene glycol 4,000 (50 w/v) 240.0 μl of water (100 v/v)
C2	Sodium acetate 0.2 M - MES 0.1 M pH 6.5 Polyethylene glycol 4,000 30 w/v - 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium acetate (2 M) 200.0 μl of water (100 v/v)
C3	Ammonium sulfate 0.2 M - tris chloride BCC 0.15 M pH 8.5 Polyethylene glycol 4,000 30 w/v - 🔍	pH 8.5	66.7 μl of Ammonium sulfate (3 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 150.0 μl of tris chloride BCC pH 8.5 (1 M) 183.3 μl of water (100 v/v)
C4	2-Propanol 10 v/v - HEPES 0.1 M pH 7.5 Polyethylene glycol monomethyl ether 5,000 8 w/v - 🔍	pH 7.5	100.0 μl of 2-Propanol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 160.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 640.0 μl of water (100 v/v)
C5	Ammonium sulfate 0.2 M - MES 0.1 M pH 6.5 Polyethylene glycol monomethyl ether 5,000 25 w/v - 🔍	pH 6.5	66.7 μl of Ammonium sulfate (3 M) 100.0 μl of MES pH 6.5 (1 M) 500.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 333.3 μl of water (100 v/v)
C6	Ammonium sulfate 0.2 M - HEPES 0.1 M pH 7.5 Polyethylene glycol monomethyl ether 5,000 30 w/v - 🔍	pH 7.5	66.7 μl of Ammonium sulfate (3 M) 100.0 μl of HEPES pH 7.5 (1 M) 600.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 233.3 μl of water (100 v/v)
D1	Ammonium sulfate 0.1 M - ADA 0.1 M pH 6.5 Polyethylene glycol monomethyl ether 5,000 30 w/v - 🔍	pH 6.5	100.0 μl of ADA pH 6.5 (1 M) 33.3 μl of Ammonium sulfate (3 M) 600.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 266.7 μl of water (100 v/v)
D2	MES 0.1 M pH 6.5 Polyethylene glycol 6,000 20 w/v - 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 500.0 μl of water (100 v/v)
D3	Dithiothreitol 0.01 M - MES 0.1 M pH 6.5 Polyethylene glycol 6,000 28 w/v - 🔍	pH 6.5	100.0 μl of Dithiothreitol (0.1 M) 100.0 μl of MES pH 6.5 (1 M) 560.0 μl of Polyethylene glycol 6,000 (50 w/v) 240.0 μl of water (100 v/v)
D4	Lithium sulfate 0.175 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 6,000 30 w/v - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 58.3 μl of Lithium sulfate (3 M) 600.0 μl of Polyethylene glycol 6,000 (50 w/v) 241.7 μl of water (100 v/v)
D5	Dithiothreitol 0.01 M - PIPES 0.1 M pH 7 Polyethylene glycol 6,000 30 w/v - 🔍	pH 7	100.0 μl of Dithiothreitol (0.1 M) 100.0 μl of PIPES pH 7 (1 M) 600.0 μl of Polyethylene glycol 6,000 (50 w/v) 200.0 μl of water (100 v/v)
D6	MES 0.1 M pH 6.5 Polyethylene glycol 6,000 32 w/v - 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 640.0 μl of Polyethylene glycol 6,000 (50 w/v) 260.0 μl of water (100 v/v)