C6 - Kinase 3 - Screen Contents

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Supplemental Information

Screen Description

JB Kinase / Kinase 3

Created 2009-11-16.

Rigaku Recipe XML

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Screen Structure

Well Count	24
Number of distinct ingredients	19
Internal diversity (Score mean dist)	0.86
Internal diversity (Ion mean dist)	0.85
Internal diversity (PEG mean dist)	0.81
Internal diversity (Known pH mean dist)	0.73
Internal diversity (Penalty mean dist)	0.84

Screen Composition

More information about screen composition chart.

Chemical classes bar chart — ingredient type Kinase 3

Buffer 26

Organic (non-volatile) 1

Organic (volatile) 3

Polymer 25

Salt 18

ingredient type	Kinase 3
Buffer	26
Organic (non-volatile)	1
Organic (volatile)	3
Polymer	25
Salt	18

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Kinase 3 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Polyethylene glycol 4,000 8 w/v - Magnesium chloride 0.01 M - Dithiothreitol 0.01 M - MES 0.05 M pH 6.5 🔍 ⚖️	pH 6.5	100.0 μl of Dithiothreitol (0.1 M) 4.0 μl of Magnesium chloride (2.5 M) 50.0 μl of MES pH 6.5 (1 M) 160.0 μl of Polyethylene glycol 4,000 (50 w/v) 686.0 μl of water (100 v/v)
A2	Polyethylene glycol 4,000 10 w/v - Dithiothreitol 0.01 M - PIPES 0.05 M pH 7 🔍 ⚖️	pH 7	100.0 μl of Dithiothreitol (0.1 M) 50.0 μl of PIPES pH 7 (1 M) 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 650.0 μl of water (100 v/v)
A3	2-Propanol 10 v/v - Ethylene glycol 15 v/v - Magnesium chloride 0.2 M - Polyethylene glycol 4,000 10 w/v - HEPES 0.1 M pH 7.5 🔍 ⚖️	pH 7.5	100.0 μl of 2-Propanol (100 v/v) 150.0 μl of Ethylene glycol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 80.0 μl of Magnesium chloride (2.5 M) 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 370.0 μl of water (100 v/v)
A4	Polyethylene glycol 4,000 10 w/v - HEPES 0.1 M pH 7.5 🔍 ⚖️	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 700.0 μl of water (100 v/v)
A5	Polyethylene glycol 4,000 15 w/v - 2-Propanol 10 v/v - HEPES 0.1 M pH 7.5 🔍 ⚖️	pH 7.5	100.0 μl of 2-Propanol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 300.0 μl of Polyethylene glycol 4,000 (50 w/v) 500.0 μl of water (100 v/v)
A6	Polyethylene glycol 4,000 15 w/v - Sodium chloride 0.2 M - Polyethylene glycol 6,000 1 w/v - Sodium acetate 0.075 M - tris chloride BCC 0.075 M pH 8.5 🔍 ⚖️	pH 8.5	300.0 μl of Polyethylene glycol 4,000 (50 w/v) 20.0 μl of Polyethylene glycol 6,000 (50 w/v) 37.5 μl of Sodium acetate (2 M) 200.0 μl of Sodium chloride (1 M) 75.0 μl of tris chloride BCC pH 8.5 (1 M) 367.5 μl of water (100 v/v)
B1	Polyethylene glycol 4,000 15 w/v - Ammonium sulfate 0.2 M - di-Sodium DL-malate 0.1 M pH 5.5 🔍 ⚖️	pH 5.5	66.67 μl of Ammonium sulfate (3 M) 11.46 μl of di-Sodium DL-malate pH 5 (3 M) 21.87 μl of di-Sodium DL-malate pH 7 (3 M) 300.0 μl of Polyethylene glycol 4,000 (50 w/v) 600.0 μl of water (100 v/v)
B2	Polyethylene glycol 4,000 20 w/v - Sodium chloride 0.1 M - BIS-TRIS 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	100.0 μl of BIS-TRIS pH 6.5 (1 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium chloride (1 M) 400.0 μl of water (100 v/v)
B3	Polyethylene glycol 4,000 20 w/v - Magnesium chloride 0.2 M - HEPES 0.1 M pH 7.5 🔍 ⚖️	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 80.0 μl of Magnesium chloride (2.5 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 420.0 μl of water (100 v/v)
B4	Polyethylene glycol 4,000 20 w/v - Magnesium chloride 0.2 M - tris chloride BCC 0.1 M pH 8.5 🔍 ⚖️	pH 8.5	80.0 μl of Magnesium chloride (2.5 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 420.0 μl of water (100 v/v)
B5	Polyethylene glycol 4,000 25 w/v - Magnesium chloride 0.2 M - MES 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	80.0 μl of Magnesium chloride (2.5 M) 100.0 μl of MES pH 6.5 (1 M) 500.0 μl of Polyethylene glycol 4,000 (50 w/v) 320.0 μl of water (100 v/v)
B6	Polyethylene glycol 4,000 25 w/v - Lithium sulfate 0.1 M - tris chloride BCC 0.1 M pH 8.5 🔍 ⚖️	pH 8.5	33.33 μl of Lithium sulfate (3 M) 500.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 366.67 μl of water (100 v/v)
C1	Polyethylene glycol 4,000 28 w/v - Lithium acetate 0.2 M pH 7.5 🔍 ⚖️	pH 7.5	200.0 μl of Lithium acetate pH 7.5 (1 M) 560.0 μl of Polyethylene glycol 4,000 (50 w/v) 240.0 μl of water (100 v/v)
C2	Polyethylene glycol 4,000 30 w/v - Sodium acetate 0.2 M - MES 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium acetate (2 M) 200.0 μl of water (100 v/v)
C3	Polyethylene glycol 4,000 30 w/v - Ammonium sulfate 0.2 M - tris chloride BCC 0.15 M pH 8.5 🔍 ⚖️	pH 8.5	66.67 μl of Ammonium sulfate (3 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 150.0 μl of tris chloride BCC pH 8.5 (1 M) 183.33 μl of water (100 v/v)
C4	2-Propanol 10 v/v - Polyethylene glycol monomethyl ether 5,000 8 w/v - HEPES 0.1 M pH 7.5 🔍 ⚖️	pH 7.5	100.0 μl of 2-Propanol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 160.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 640.0 μl of water (100 v/v)
C5	Polyethylene glycol monomethyl ether 5,000 25 w/v - Ammonium sulfate 0.2 M - MES 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	66.67 μl of Ammonium sulfate (3 M) 100.0 μl of MES pH 6.5 (1 M) 500.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 333.33 μl of water (100 v/v)
C6	Polyethylene glycol monomethyl ether 5,000 30 w/v - Ammonium sulfate 0.2 M - HEPES 0.1 M pH 7.5 🔍 ⚖️	pH 7.5	66.67 μl of Ammonium sulfate (3 M) 100.0 μl of HEPES pH 7.5 (1 M) 600.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 233.33 μl of water (100 v/v)
D1	Polyethylene glycol monomethyl ether 5,000 30 w/v - Ammonium sulfate 0.1 M - ADA 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	100.0 μl of ADA pH 6.5 (1 M) 33.33 μl of Ammonium sulfate (3 M) 600.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 266.67 μl of water (100 v/v)
D2	Polyethylene glycol 6,000 20 w/v - MES 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 500.0 μl of water (100 v/v)
D3	Polyethylene glycol 6,000 28 w/v - Dithiothreitol 0.01 M - MES 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	100.0 μl of Dithiothreitol (0.1 M) 100.0 μl of MES pH 6.5 (1 M) 560.0 μl of Polyethylene glycol 6,000 (50 w/v) 240.0 μl of water (100 v/v)
D4	Polyethylene glycol 6,000 30 w/v - Lithium sulfate 0.175 M - HEPES 0.1 M pH 7.5 🔍 ⚖️	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 58.33 μl of Lithium sulfate (3 M) 600.0 μl of Polyethylene glycol 6,000 (50 w/v) 241.67 μl of water (100 v/v)
D5	Polyethylene glycol 6,000 30 w/v - Dithiothreitol 0.01 M - PIPES 0.1 M pH 7 🔍 ⚖️	pH 7	100.0 μl of Dithiothreitol (0.1 M) 100.0 μl of PIPES pH 7 (1 M) 600.0 μl of Polyethylene glycol 6,000 (50 w/v) 200.0 μl of water (100 v/v)
D6	Polyethylene glycol 6,000 32 w/v - MES 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 640.0 μl of Polyethylene glycol 6,000 (50 w/v) 260.0 μl of water (100 v/v)