C6 - Screen Contents

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Supplemental Information

Screen Description

Kinase 3

Kinase / Kinase 3

Created 2009-11-16.

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Kinase 3

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Kinase 3

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Screen Structure

Kinase 3

Well Count	24
Number of distinct ingredients	19
Internal diversity (score mean dist)	0.860

Screen Composition

More information about screen composition chart.

ingredient type	Kinase 3
Buffer	26
Organic (non-volatile)	1
Organic (volatile)	3
Polymer	25
Salt	18

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Kinase 3 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Magnesium chloride 0.01 M - Dithiothreitol 0.01 M - Polyethylene glycol 4,000 8 w/v - MES 0.05 M pH 6.5 🔍	pH 6.5	100.0 μl of Dithiothreitol (0.1 M) 50.0 μl of MES pH 6.5 (1 M) 4.0 μl of Magnesium chloride (2.5 M) 160.0 μl of Polyethylene glycol 4,000 (50 w/v) 686.0 μl of water (100 v/v)
A2	Dithiothreitol 0.01 M - PIPES 0.05 M pH 7 Polyethylene glycol 4,000 10 w/v - 🔍	pH 7	100.0 μl of Dithiothreitol (0.1 M) 50.0 μl of PIPES pH 7 (1 M) 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 650.0 μl of water (100 v/v)
A3	Ethylene glycol 15 v/v - 2-Propanol 10 v/v - Magnesium chloride 0.2 M - Polyethylene glycol 4,000 10 w/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100.0 μl of 2-Propanol (100 v/v) 150.0 μl of Ethylene glycol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 80.0 μl of Magnesium chloride (2.5 M) 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 370.0 μl of water (100 v/v)
A4	HEPES 0.1 M pH 7.5 Polyethylene glycol 4,000 10 w/v - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 700.0 μl of water (100 v/v)
A5	2-Propanol 10 v/v - HEPES 0.1 M pH 7.5 Polyethylene glycol 4,000 15 w/v - 🔍	pH 7.5	100.0 μl of 2-Propanol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 300.0 μl of Polyethylene glycol 4,000 (50 w/v) 500.0 μl of water (100 v/v)
A6	Sodium chloride 0.2 M - Polyethylene glycol 6,000 1 w/v - Sodium acetate 0.075 M - Polyethylene glycol 4,000 15 w/v - tris chloride BCC 0.075 M pH 8.5 🔍	pH 8.5	300.0 μl of Polyethylene glycol 4,000 (50 w/v) 20.0 μl of Polyethylene glycol 6,000 (50 w/v) 37.5 μl of Sodium acetate (2 M) 200.0 μl of Sodium chloride (1 M) 75.0 μl of tris chloride BCC pH 8.5 (1 M) 367.5 μl of water (100 v/v)
B1	Ammonium sulfate 0.2 M - di-Sodium DL-malate 0.1 M pH 5.5 Polyethylene glycol 4,000 15 w/v - 🔍	pH 5.5	66.7 μl of Ammonium sulfate (3 M) 300.0 μl of Polyethylene glycol 4,000 (50 w/v) 11.5 μl of di-Sodium DL-malate pH 5 (3 M) 21.9 μl of di-Sodium DL-malate pH 7 (3 M) 600.0 μl of water (100 v/v)
B2	Sodium chloride 0.1 M - BIS-TRIS 0.1 M pH 6.5 Polyethylene glycol 4,000 20 w/v - 🔍	pH 6.5	100.0 μl of BIS-TRIS pH 6.5 (1 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium chloride (1 M) 400.0 μl of water (100 v/v)
B3	Magnesium chloride 0.2 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 4,000 20 w/v - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 80.0 μl of Magnesium chloride (2.5 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 420.0 μl of water (100 v/v)
B4	Magnesium chloride 0.2 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 4,000 20 w/v - 🔍	pH 8.5	80.0 μl of Magnesium chloride (2.5 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 420.0 μl of water (100 v/v)
B5	Magnesium chloride 0.2 M - MES 0.1 M pH 6.5 Polyethylene glycol 4,000 25 w/v - 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 80.0 μl of Magnesium chloride (2.5 M) 500.0 μl of Polyethylene glycol 4,000 (50 w/v) 320.0 μl of water (100 v/v)
B6	Lithium sulfate 0.1 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 4,000 25 w/v - 🔍	pH 8.5	33.3 μl of Lithium sulfate (3 M) 500.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 366.7 μl of water (100 v/v)
C1	Lithium acetate 0.2 M pH 7.5 Polyethylene glycol 4,000 28 w/v - 🔍	pH 7.5	200.0 μl of Lithium acetate pH 7.5 (1 M) 560.0 μl of Polyethylene glycol 4,000 (50 w/v) 240.0 μl of water (100 v/v)
C2	Sodium acetate 0.2 M - MES 0.1 M pH 6.5 Polyethylene glycol 4,000 30 w/v - 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium acetate (2 M) 200.0 μl of water (100 v/v)
C3	Ammonium sulfate 0.2 M - tris chloride BCC 0.15 M pH 8.5 Polyethylene glycol 4,000 30 w/v - 🔍	pH 8.5	66.7 μl of Ammonium sulfate (3 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 150.0 μl of tris chloride BCC pH 8.5 (1 M) 183.3 μl of water (100 v/v)
C4	2-Propanol 10 v/v - HEPES 0.1 M pH 7.5 Polyethylene glycol monomethyl ether 5,000 8 w/v - 🔍	pH 7.5	100.0 μl of 2-Propanol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 160.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 640.0 μl of water (100 v/v)
C5	Ammonium sulfate 0.2 M - MES 0.1 M pH 6.5 Polyethylene glycol monomethyl ether 5,000 25 w/v - 🔍	pH 6.5	66.7 μl of Ammonium sulfate (3 M) 100.0 μl of MES pH 6.5 (1 M) 500.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 333.3 μl of water (100 v/v)
C6	Ammonium sulfate 0.2 M - HEPES 0.1 M pH 7.5 Polyethylene glycol monomethyl ether 5,000 30 w/v - 🔍	pH 7.5	66.7 μl of Ammonium sulfate (3 M) 100.0 μl of HEPES pH 7.5 (1 M) 600.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 233.3 μl of water (100 v/v)
D1	Ammonium sulfate 0.1 M - ADA 0.1 M pH 6.5 Polyethylene glycol monomethyl ether 5,000 30 w/v - 🔍	pH 6.5	100.0 μl of ADA pH 6.5 (1 M) 33.3 μl of Ammonium sulfate (3 M) 600.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 266.7 μl of water (100 v/v)
D2	MES 0.1 M pH 6.5 Polyethylene glycol 6,000 20 w/v - 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 500.0 μl of water (100 v/v)
D3	Dithiothreitol 0.01 M - MES 0.1 M pH 6.5 Polyethylene glycol 6,000 28 w/v - 🔍	pH 6.5	100.0 μl of Dithiothreitol (0.1 M) 100.0 μl of MES pH 6.5 (1 M) 560.0 μl of Polyethylene glycol 6,000 (50 w/v) 240.0 μl of water (100 v/v)
D4	Lithium sulfate 0.175 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 6,000 30 w/v - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 58.3 μl of Lithium sulfate (3 M) 600.0 μl of Polyethylene glycol 6,000 (50 w/v) 241.7 μl of water (100 v/v)
D5	Dithiothreitol 0.01 M - PIPES 0.1 M pH 7 Polyethylene glycol 6,000 30 w/v - 🔍	pH 7	100.0 μl of Dithiothreitol (0.1 M) 100.0 μl of PIPES pH 7 (1 M) 600.0 μl of Polyethylene glycol 6,000 (50 w/v) 200.0 μl of water (100 v/v)
D6	MES 0.1 M pH 6.5 Polyethylene glycol 6,000 32 w/v - 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 640.0 μl of Polyethylene glycol 6,000 (50 w/v) 260.0 μl of water (100 v/v)