C6 - Kinase 4 - Screen Contents

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Supplemental Information

Screen Description

JB Kinase / Kinase 4

Created 2009-11-16.

Rigaku Recipe XML

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Screen Structure

Well Count	24
Number of distinct ingredients	24
Internal diversity (Score mean dist)	0.84
Internal diversity (Ion mean dist)	0.84
Internal diversity (PEG mean dist)	0.79
Internal diversity (Known pH mean dist)	0.72
Internal diversity (Penalty mean dist)	0.83

Screen Composition

More information about screen composition chart.

Chemical classes bar chart — ingredient type Kinase 4

Buffer 27

Cryoprotectant 1

Organic (non-volatile) 8

Organic (volatile) 1

Polymer 25

Salt 14

ingredient type	Kinase 4
Buffer	27
Cryoprotectant	1
Organic (non-volatile)	8
Organic (volatile)	1
Polymer	25
Salt	14

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Kinase 4 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Ethylene glycol 20 v/v - Polyethylene glycol 8,000 7 w/v - MES 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	200.0 μl of Ethylene glycol (100 v/v) 100.0 μl of MES pH 6.5 (1 M) 140.0 μl of Polyethylene glycol 8,000 (50 w/v) 560.0 μl of water (100 v/v)
A2	Ethylene glycol 16 v/v - Calcium acetate 0.15 M - Polyethylene glycol 8,000 7 w/v - MES 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	75.0 μl of Calcium acetate (2 M) 160.0 μl of Ethylene glycol (100 v/v) 100.0 μl of MES pH 6.5 (1 M) 140.0 μl of Polyethylene glycol 8,000 (50 w/v) 525.0 μl of water (100 v/v)
A3	Polyethylene glycol 8,000 10 w/v - Polyethylene glycol 200 10 v/v - tris chloride BCC 0.1 M pH 8.5 🔍 ⚖️	pH 8.5	100.0 μl of Polyethylene glycol 200 (100 v/v) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 600.0 μl of water (100 v/v)
A4	Polyethylene glycol 8,000 12 w/v - HEPES 0.1 M pH 7.5 🔍 ⚖️	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 240.0 μl of Polyethylene glycol 8,000 (50 w/v) 660.0 μl of water (100 v/v)
A5	Polyethylene glycol 8,000 12 w/v - di-Sodium tartrate 0.25 M - tris chloride BCC 0.1 M pH 8.5 🔍 ⚖️	pH 8.5	125.0 μl of di-Sodium tartrate (2 M) 240.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 535.0 μl of water (100 v/v)
A6	Polyethylene glycol 8,000 16 w/v - Potassium di-hydrogen phosphate 0.1 M - HEPES 0.1 M pH 7.5 🔍 ⚖️	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 320.0 μl of Polyethylene glycol 8,000 (50 w/v) 50.0 μl of Potassium di-hydrogen phosphate (2 M) 530.0 μl of water (100 v/v)
B1	Polyethylene glycol 8,000 16 w/v - Sodium chloride 0.15 M - Ethylene glycol 2 v/v - HEPES 0.1 M pH 7.5 🔍 ⚖️	pH 7.5	40.0 μl of Ethylene glycol (50 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 320.0 μl of Polyethylene glycol 8,000 (50 w/v) 150.0 μl of Sodium chloride (1 M) 390.0 μl of water (100 v/v)
B2	Polyethylene glycol 8,000 18 w/v - Magnesium acetate 0.2 M - MES 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	100.0 μl of Magnesium acetate (2 M) 100.0 μl of MES pH 6.5 (1 M) 360.0 μl of Polyethylene glycol 8,000 (50 w/v) 440.0 μl of water (100 v/v)
B3	Polyethylene glycol 8,000 18 w/v - MES 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 360.0 μl of Polyethylene glycol 8,000 (50 w/v) 540.0 μl of water (100 v/v)
B4	Polyethylene glycol 8,000 18 w/v - tris chloride BCC 0.1 M pH 8.5 🔍 ⚖️	pH 8.5	360.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 540.0 μl of water (100 v/v)
B5	Polyethylene glycol 8,000 20 w/v - Magnesium acetate 0.1 M - tri-Sodium citrate 0.1 M pH 5 🔍 ⚖️	pH 5	50.0 μl of Magnesium acetate (2 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 450.0 μl of water (100 v/v)
B6	Polyethylene glycol 8,000 20 w/v - Magnesium chloride 0.2 M - Ethylene glycol 2 v/v - tris chloride BCC 0.1 M pH 8.5 🔍 ⚖️	pH 8.5	20.0 μl of Ethylene glycol (100 v/v) 80.0 μl of Magnesium chloride (2.5 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 400.0 μl of water (100 v/v)
C1	Polyethylene glycol 8,000 22 w/v - Ethylene glycol 2 v/v - tris chloride BCC 0.1 M pH 8.5 🔍 ⚖️	pH 8.5	20.0 μl of Ethylene glycol (100 v/v) 440.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 440.0 μl of water (100 v/v)
C2	Polyethylene glycol 8,000 25 w/v - Magnesium chloride 0.05 M - Sodium acetate 0.1 M pH 4.6 🔍 ⚖️	pH 4.6	20.0 μl of Magnesium chloride (2.5 M) 500.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 380.0 μl of water (100 v/v)
C3	Polyethylene glycol 8,000 30 w/v - Ammonium sulfate 0.2 M - 1,3-Propanediol 4 v/v - MES 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	80.0 μl of 1,3-Propanediol (50 v/v) 66.67 μl of Ammonium sulfate (3 M) 100.0 μl of MES pH 6.5 (1 M) 600.0 μl of Polyethylene glycol 8,000 (50 w/v) 153.33 μl of water (100 v/v)
C4	Polyethylene glycol 8,000 30 w/v - Dithiothreitol 0.01 M - Glycerol 20 v/v - HEPES 0.1 M pH 7.5 🔍 ⚖️	pH 7.5	100.0 μl of Dithiothreitol (0.1 M) 200.0 μl of Glycerol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 600.0 μl of Polyethylene glycol 8,000 (50 w/v)
C5	Zinc acetate 0.2 M - Polyethylene glycol 8,000 9 w/v - MES 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 180.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Zinc acetate (2 M) 620.0 μl of water (100 v/v)
C6	Polyethylene glycol 10,000 16 w/v - Ammonium sulfate 0.3 M - Ethylene glycol 5 v/v - BIS-TRIS 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	100.0 μl of Ammonium sulfate (3 M) 100.0 μl of BIS-TRIS pH 6.5 (1 M) 100.0 μl of Ethylene glycol (50 v/v) 320.0 μl of Polyethylene glycol 10,000 (50 w/v) 380.0 μl of water (100 v/v)
D1	Polyethylene glycol 10,000 10 w/v - Ethylene glycol 8 v/v - HEPES 0.1 M pH 7.5 🔍 ⚖️	pH 7.5	80.0 μl of Ethylene glycol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 10,000 (50 w/v) 620.0 μl of water (100 v/v)
D2	Polyethylene glycol 10,000 15 w/v - Dithiothreitol 0.005 M - HEPES 0.1 M pH 7.5 🔍 ⚖️	pH 7.5	50.0 μl of Dithiothreitol (0.1 M) 100.0 μl of HEPES pH 7.5 (1 M) 300.0 μl of Polyethylene glycol 10,000 (50 w/v) 550.0 μl of water (100 v/v)
D3	Polyethylene glycol 10,000 15 w/v - tris chloride BCC 0.1 M pH 8.5 🔍 ⚖️	pH 8.5	300.0 μl of Polyethylene glycol 10,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 600.0 μl of water (100 v/v)
D4	Polyethylene glycol 20,000 12 w/v - MES 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 240.0 μl of Polyethylene glycol 20,000 (50 w/v) 660.0 μl of water (100 v/v)
D5	Polyethylene glycol 20,000 10 w/v - Ammonium formate 0.1 M - HEPES 0.1 M pH 7.5 🔍 ⚖️	pH 7.5	40.0 μl of Ammonium formate (2.5 M) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 20,000 (50 w/v) 660.0 μl of water (100 v/v)
D6	Potassium hydrogen tartrate 0.01 M - Polyethylene glycol 20,000 15 w/v - 🔍 ⚖️	NA	300.0 μl of Polyethylene glycol 20,000 (50 w/v) 100.0 μl of Potassium hydrogen tartrate (0.1 M) 600.0 μl of water (100 v/v)