C6 - Screen Contents

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Supplemental Information

Screen Description

Kinase 4

Kinase / Kinase 4

Created 2009-11-16.

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Kinase 4

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Kinase 4

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Screen Structure

Kinase 4

Well Count	24
Number of distinct ingredients	24
Internal diversity (score mean dist)	0.845

Screen Composition

More information about screen composition chart.

ingredient type	Kinase 4
Buffer	27
Cryoprotectant	1
Organic (non-volatile)	8
Organic (volatile)	1
Polymer	25
Salt	14

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Kinase 4 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Ethylene glycol 20 v/v - MES 0.1 M pH 6.5 Polyethylene glycol 8,000 7 w/v - 🔍	pH 6.5	200.0 μl of Ethylene glycol (100 v/v) 100.0 μl of MES pH 6.5 (1 M) 140.0 μl of Polyethylene glycol 8,000 (50 w/v) 560.0 μl of water (100 v/v)
A2	Calcium acetate 0.15 M - Ethylene glycol 16 v/v - Polyethylene glycol 8,000 7 w/v - MES 0.1 M pH 6.5 🔍	pH 6.5	75.0 μl of Calcium acetate (2 M) 160.0 μl of Ethylene glycol (100 v/v) 100.0 μl of MES pH 6.5 (1 M) 140.0 μl of Polyethylene glycol 8,000 (50 w/v) 525.0 μl of water (100 v/v)
A3	Polyethylene glycol 200 10 v/v - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 8,000 10 w/v - 🔍	pH 8.5	100.0 μl of Polyethylene glycol 200 (100 v/v) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 600.0 μl of water (100 v/v)
A4	HEPES 0.1 M pH 7.5 Polyethylene glycol 8,000 12 w/v - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 240.0 μl of Polyethylene glycol 8,000 (50 w/v) 660.0 μl of water (100 v/v)
A5	di-Sodium tartrate 0.25 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 8,000 12 w/v - 🔍	pH 8.5	240.0 μl of Polyethylene glycol 8,000 (50 w/v) 125.0 μl of di-Sodium tartrate (2 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 535.0 μl of water (100 v/v)
A6	Potassium di-hydrogen phosphate 0.1 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 8,000 16 w/v - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 320.0 μl of Polyethylene glycol 8,000 (50 w/v) 50.0 μl of Potassium di-hydrogen phosphate (2 M) 530.0 μl of water (100 v/v)
B1	Sodium chloride 0.15 M - Ethylene glycol 2 v/v - Polyethylene glycol 8,000 16 w/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	40.0 μl of Ethylene glycol (50 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 320.0 μl of Polyethylene glycol 8,000 (50 w/v) 150.0 μl of Sodium chloride (1 M) 390.0 μl of water (100 v/v)
B2	Magnesium acetate 0.2 M - MES 0.1 M pH 6.5 Polyethylene glycol 8,000 18 w/v - 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 100.0 μl of Magnesium acetate (2 M) 360.0 μl of Polyethylene glycol 8,000 (50 w/v) 440.0 μl of water (100 v/v)
B3	MES 0.1 M pH 6.5 Polyethylene glycol 8,000 18 w/v - 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 360.0 μl of Polyethylene glycol 8,000 (50 w/v) 540.0 μl of water (100 v/v)
B4	tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 8,000 18 w/v - 🔍	pH 8.5	360.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 540.0 μl of water (100 v/v)
B5	Magnesium acetate 0.1 M - tri-Sodium citrate 0.1 M pH 5 Polyethylene glycol 8,000 20 w/v - 🔍	pH 5	50.0 μl of Magnesium acetate (2 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 450.0 μl of water (100 v/v)
B6	Magnesium chloride 0.2 M - Ethylene glycol 2 v/v - Polyethylene glycol 8,000 20 w/v - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	20.0 μl of Ethylene glycol (100 v/v) 80.0 μl of Magnesium chloride (2.5 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 400.0 μl of water (100 v/v)
C1	Ethylene glycol 2 v/v - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 8,000 22 w/v - 🔍	pH 8.5	20.0 μl of Ethylene glycol (100 v/v) 440.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 440.0 μl of water (100 v/v)
C2	Magnesium chloride 0.05 M - Sodium acetate 0.1 M pH 4.6 Polyethylene glycol 8,000 25 w/v - 🔍	pH 4.6	20.0 μl of Magnesium chloride (2.5 M) 500.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 380.0 μl of water (100 v/v)
C3	Ammonium sulfate 0.2 M - 1,3-Propanediol 4 v/v - Polyethylene glycol 8,000 30 w/v - MES 0.1 M pH 6.5 🔍	pH 6.5	80.0 μl of 1,3-Propanediol (50 v/v) 66.7 μl of Ammonium sulfate (3 M) 100.0 μl of MES pH 6.5 (1 M) 600.0 μl of Polyethylene glycol 8,000 (50 w/v) 153.3 μl of water (100 v/v)
C4	Dithiothreitol 0.01 M - Glycerol 20 v/v - Polyethylene glycol 8,000 30 w/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100.0 μl of Dithiothreitol (0.1 M) 200.0 μl of Glycerol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 600.0 μl of Polyethylene glycol 8,000 (50 w/v)
C5	Zinc acetate 0.2 M - MES 0.1 M pH 6.5 Polyethylene glycol 8,000 9 w/v - 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 180.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Zinc acetate (2 M) 620.0 μl of water (100 v/v)
C6	Ammonium sulfate 0.3 M - Ethylene glycol 5 v/v - Polyethylene glycol 10,000 16 w/v - BIS-TRIS 0.1 M pH 6.5 🔍	pH 6.5	100.0 μl of Ammonium sulfate (3 M) 100.0 μl of BIS-TRIS pH 6.5 (1 M) 100.0 μl of Ethylene glycol (50 v/v) 320.0 μl of Polyethylene glycol 10,000 (50 w/v) 380.0 μl of water (100 v/v)
D1	Ethylene glycol 8 v/v - HEPES 0.1 M pH 7.5 Polyethylene glycol 10,000 10 w/v - 🔍	pH 7.5	80.0 μl of Ethylene glycol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 10,000 (50 w/v) 620.0 μl of water (100 v/v)
D2	Dithiothreitol 0.005 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 10,000 15 w/v - 🔍	pH 7.5	50.0 μl of Dithiothreitol (0.1 M) 100.0 μl of HEPES pH 7.5 (1 M) 300.0 μl of Polyethylene glycol 10,000 (50 w/v) 550.0 μl of water (100 v/v)
D3	tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 10,000 15 w/v - 🔍	pH 8.5	300.0 μl of Polyethylene glycol 10,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 600.0 μl of water (100 v/v)
D4	MES 0.1 M pH 6.5 Polyethylene glycol 20,000 12 w/v - 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 240.0 μl of Polyethylene glycol 20,000 (50 w/v) 660.0 μl of water (100 v/v)
D5	Ammonium formate 0.1 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 20,000 10 w/v - 🔍	pH 7.5	40.0 μl of Ammonium formate (2.5 M) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 20,000 (50 w/v) 660.0 μl of water (100 v/v)
D6	Potassium hydrogen tartrate 0.01 M - Polyethylene glycol 20,000 15 w/v - 🔍	NA	300.0 μl of Polyethylene glycol 20,000 (50 w/v) 100.0 μl of Potassium hydrogen tartrate (0.1 M) 600.0 μl of water (100 v/v)