Jump to page contents
Welcome to the new C6 website. This website aims preserve as many features as possible of the original C6 website.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.

Supplemental Information

Screen Description

Kinase HTS

Kinase / Kinase HTS
Created 2009-11-16.

Export

Barcodes

Kinase HTS

    Screen Structure

    Kinase HTS

    Well Count 96
    Number of distinct ingredients 64
    Internal diversity (score mean dist) 0.936

    Screen Composition

    2025-06-13T21:31:49.255831 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Kinase HTS
    Buffer 111
    Cryoprotectant 3
    Organic (non-volatile) 20
    Organic (volatile) 6
    Polymer 77
    Salt 101

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Kinase HTS Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Magnesium acetate 0.2 M -
    • Dithiothreitol 0.01 M -
    • Ammonium sulfate 1 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    333 μl of Ammonium sulfate (3 M)
    100 μl of Magnesium acetate (2 M)
    100 μl of Dithiothreitol (0.1 M)
    366 μl of water (100 v/v)
    A2
    • tri-Sodium citrate 0.1 M pH 5.6
    • Ammonium sulfate 1.3 M -
    pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    433 μl of Ammonium sulfate (3 M)
    466 μl of water (100 v/v)
    A3
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium sulfate 1.3 M -
    pH 8.5
    433 μl of Ammonium sulfate (3 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    466 μl of water (100 v/v)
    A4
    • Cobalt (II) chloride 0.025 M -
    • MES 0.1 M pH 6.5
    • Ammonium sulfate 1.8 M -
    pH 6.5
    25 μl of Cobalt (II) chloride (1 M)
    100 μl of MES pH 6.5 (1 M)
    600 μl of Ammonium sulfate (3 M)
    275 μl of water (100 v/v)
    A5
    • Sodium chloride 0.2 M -
    • tri-Sodium citrate 0.1 M pH 3.1
    • Ammonium sulfate 2 M -
    pH 3.1
    100 μl of tri-Sodium citrate pH 3.1 (1 M)
    200 μl of Sodium chloride (1 M)
    666 μl of Ammonium sulfate (3 M)
    33 μl of water (100 v/v)
    A6
    • Magnesium chloride 0.05 M -
    • Sodium acetate 0.1 M pH 4.6
    • Ammonium sulfate 2 M -
    pH 4.6
    20 μl of Magnesium chloride (2.5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    666 μl of Ammonium sulfate (3 M)
    213 μl of water (100 v/v)
    A7
    • Sodium acetate 0.1 M pH 4.6
    • Ammonium sulfate 2 M -
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    666 μl of Ammonium sulfate (3 M)
    233 μl of water (100 v/v)
    A8
    • Polyethylene glycol monomethyl ether 550 2 v/v -
    • HEPES 0.1 M pH 7.5
    • Ammonium sulfate 2 M -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    20 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    666 μl of Ammonium sulfate (3 M)
    213 μl of water (100 v/v)
    A9
    • HEPES 0.1 M pH 7.5
    • Ammonium sulfate 2 M -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    666 μl of Ammonium sulfate (3 M)
    233 μl of water (100 v/v)
    A10
    • Magnesium chloride 0.006 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium sulfate 2 M -
    pH 8.5
    2 μl of Magnesium chloride (2.5 M)
    666 μl of Ammonium sulfate (3 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    230 μl of water (100 v/v)
    A11
    • Nickel sulfate 0.01 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Lithium sulfate 1.5 M -
    pH 8.5
    2 μl of Nickel sulfate (4 M)
    500 μl of Lithium sulfate (3 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    397 μl of water (100 v/v)
    A12
    • tri-Sodium citrate 0.1 M pH 4.2
    • Lithium chloride 1 M -
    pH 4.2
    200 μl of Lithium chloride (5 M)
    100 μl of tri-Sodium citrate pH 4.2 (1 M)
    700 μl of water (100 v/v)
    B1
    • Sodium acetate 0.1 M pH 4.6
    • Sodium chloride 2 M -
    pH 4.6
    400 μl of Sodium chloride (5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    500 μl of water (100 v/v)
    B2
    • di-Sodium hydrogen phosphate 0.1 M -
    • MES 0.1 M pH 6.5
    • Sodium chloride 2 M -
    • Potassium di-hydrogen phosphate 0.1 M -
    pH 6.5
    50 μl of Potassium di-hydrogen phosphate (2 M)
    400 μl of Sodium chloride (5 M)
    100 μl of MES pH 6.5 (1 M)
    20 μl of di-Sodium hydrogen phosphate (5 M)
    430 μl of water (100 v/v)
    B3
    • Glycerol 1 v/v -
    • HEPES 0.1 M pH 7.5
    • Sodium chloride 3.3 M -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    660 μl of Sodium chloride (5 M)
    10 μl of Glycerol (100 v/v)
    230 μl of water (100 v/v)
    B4
    • Calcium chloride 0.0625 M -
    • MES 0.1 M pH 6.5
    • Sodium acetate 1.2 M -
    pH 6.5
    600 μl of Sodium acetate (2 M)
    62 μl of Calcium chloride (1 M)
    100 μl of MES pH 6.5 (1 M)
    237 μl of water (100 v/v)
    B5
    • Polyethylene glycol 3,000 2 w/v -
    • BICINE 0.1 M pH 9.5
    • Sodium formate 3.7 M -
    pH 9.5
    528 μl of Sodium formate (7 M)
    40 μl of Polyethylene glycol 3,000 (50 w/v)
    100 μl of BICINE pH 9.5 (1 M)
    331 μl of water (100 v/v)
    B6
    • Glycerol 1.6 v/v -
    • Dithiothreitol 0.01 M -
    • di-Sodium malonate 0.5 M -
    • PIPES 0.05 M pH 6
    pH 6
    166 μl of di-Sodium malonate pH 6 (3 M)
    50 μl of PIPES pH 6 (1 M)
    16 μl of Glycerol (100 v/v)
    100 μl of Dithiothreitol (0.1 M)
    667 μl of water (100 v/v)
    B7
    • Potassium di-hydrogen phosphate 0.5 M -
    • Lithium sulfate 0.2 M -
    • di-Sodium hydrogen phosphate 0.5 M -
    • CAPS 0.1 M pH 10
    pH 10
    100 μl of CAPS pH 10 (1 M)
    250 μl of Potassium di-hydrogen phosphate (2 M)
    66 μl of Lithium sulfate (3 M)
    100 μl of di-Sodium hydrogen phosphate (5 M)
    483 μl of water (100 v/v)
    B8
    • Dithiothreitol 0.005 M -
    • tris chloride BCC 0.1 M pH 8.5
    • di-Sodium tartrate 1.2 M -
    pH 8.5
    600 μl of di-Sodium tartrate (2 M)
    50 μl of Dithiothreitol (0.1 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    250 μl of water (100 v/v)
    B9
    • MES 0.1 M pH 6.5
    • Potassium Sodium tartrate 1 M -
    pH 6.5
    500 μl of Potassium Sodium tartrate (2 M)
    100 μl of MES pH 6.5 (1 M)
    400 μl of water (100 v/v)
    B10
    • Cesium chloride 0.05 M -
    • MES 0.1 M pH 6.5
    • Jeffamine® M-600 30 v/v -
    pH 6.5
    50 μl of Cesium chloride (1 M)
    600 μl of Jeffamine® M-600 (50 v/v)
    100 μl of MES pH 6.5 (1 M)
    250 μl of water (100 v/v)
    B11
    • MES 0.1 M pH 6.5
    • 2-methyl-2,4-pentanediol BCC 40 v/v -
    pH 6.5
    400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of MES pH 6.5 (1 M)
    500 μl of water (100 v/v)
    B12
    • HEPES 0.1 M pH 7.5
    • 2-methyl-2,4-pentanediol BCC 50 v/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    400 μl of water (100 v/v)
    C1
    • Dithiothreitol 0.001 M -
    • Ethylenediaminetetraacetic acid disodium salt 1 mM -
    • Polyethylene glycol 400 10 v/v -
    • tris chloride BCC 0.05 M pH 8.5
    • Sodium chloride 0.3 M -
    pH 8.5
    142 μl of Ethylenediaminetetraacetic acid disodium salt (7 mM)
    60 μl of Sodium chloride (5 M)
    100 μl of Polyethylene glycol 400 (100 v/v)
    10 μl of Dithiothreitol (0.1 M)
    50 μl of tris chloride BCC pH 8.5 (1 M)
    637 μl of water (100 v/v)
    C2
    • Calcium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 15 v/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    200 μl of Calcium chloride (1 M)
    150 μl of Polyethylene glycol 400 (100 v/v)
    550 μl of water (100 v/v)
    C3
    • 2-Propanol 10 v/v -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 400 25 v/v -
    pH 6.5
    100 μl of 2-Propanol (100 v/v)
    250 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of MES pH 6.5 (1 M)
    550 μl of water (100 v/v)
    C4
    • tri-Sodium citrate 0.15 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 400 25 v/v -
    pH 8.5
    250 μl of Polyethylene glycol 400 (100 v/v)
    75 μl of tri-Sodium citrate (2 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    575 μl of water (100 v/v)
    C5
    • Ethylene glycol 5 v/v -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol monomethyl ether 550 15 v/v -
    pH 4.6
    100 μl of Ethylene glycol (50 v/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    150 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    650 μl of water (100 v/v)
    C6
    • Sodium chloride 0.1 M -
    • BICINE 0.1 M pH 9
    • Polyethylene glycol monomethyl ether 550 20 v/v -
    pH 9
    100 μl of BICINE pH 9 (1 M)
    100 μl of Sodium chloride (1 M)
    200 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    600 μl of water (100 v/v)
    C7
    • Dithiothreitol 0.001 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 1,000 20 w/v -
    pH 8.5
    10 μl of Dithiothreitol (0.1 M)
    400 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    490 μl of water (100 v/v)
    C8
    • Lithium sulfate 0.05 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 1,000 35 w/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    16 μl of Lithium sulfate (3 M)
    700 μl of Polyethylene glycol 1,000 (50 w/v)
    183 μl of water (100 v/v)
    C9
    • Magnesium acetate 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 2,000 12 w/v -
    pH 6.5
    240 μl of Polyethylene glycol 2,000 (50 w/v)
    100 μl of MES pH 6.5 (1 M)
    100 μl of Magnesium acetate (2 M)
    560 μl of water (100 v/v)
    C10
    • Magnesium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 2,000 25 w/v -
    pH 4.6
    40 μl of Magnesium chloride (2.5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    500 μl of Polyethylene glycol 2,000 (50 w/v)
    360 μl of water (100 v/v)
    C11
    • Magnesium chloride 0.05 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 2,000 30 w/v -
    pH 4.6
    20 μl of Magnesium chloride (2.5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    600 μl of Polyethylene glycol 2,000 (50 w/v)
    280 μl of water (100 v/v)
    C12
    • Sodium phosphate citrate 0.1 M pH 5
    • Polyethylene glycol monomethyl ether 2,000 24 w/v -
    pH 5
    480 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of Sodium phosphate citrate pH 5 (1 M)
    420 μl of water (100 v/v)
    D1
    • Sodium chloride 0.5 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 3,350 12 w/v -
    pH 6.5
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Sodium chloride (5 M)
    100 μl of MES pH 6.5 (1 M)
    560 μl of water (100 v/v)
    D2
    • Ammonium sulfate 0.2 M -
    • Magnesium sulfate 0.05 M -
    • Polyethylene glycol 3,350 12 w/v -
    • tri-Sodium citrate 0.05 M pH 5.6
    pH 5.6
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    50 μl of tri-Sodium citrate pH 5.6 (1 M)
    66 μl of Ammonium sulfate (3 M)
    50 μl of Magnesium sulfate (1 M)
    593 μl of water (100 v/v)
    D3
    • Ammonium sulfate 0.25 M -
    • Cadmium chloride 0.01 M -
    • Polyethylene glycol 3,350 15 w/v -
    • Imidazole 0.1 M pH 7.5
    pH 7.5
    10 μl of Cadmium chloride (1 M)
    100 μl of Imidazole pH 7.5 (1 M)
    300 μl of Polyethylene glycol 3,350 (50 w/v)
    83 μl of Ammonium sulfate (3 M)
    506 μl of water (100 v/v)
    D4
    • di-Sodium L-malate 0.15 M pH 7
    • Polyethylene glycol 3,350 20 w/v -
    pH 7
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    150 μl of di-Sodium L-malate pH 7 (1 M)
    450 μl of water (100 v/v)
    D5
    • Sodium chloride 0.2 M -
    • L-Glutathione reduced 0.02 M -
    • Polyethylene glycol 3,350 20 w/v -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    40 μl of Sodium chloride (5 M)
    100 μl of L-Glutathione reduced (0.2 M)
    360 μl of water (100 v/v)
    D6
    • Sodium chloride 0.12 M -
    • Dithiothreitol 0.005 M -
    • Polyethylene glycol 3,350 20 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    24 μl of Sodium chloride (5 M)
    50 μl of Dithiothreitol (0.1 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    426 μl of water (100 v/v)
    D7
    • Potassium nitrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    200 μl of Potassium nitrate (1 M)
    400 μl of water (100 v/v)
    D8
    • Ammonium formate 0.1 M -
    • Polyethylene glycol 3,350 22 w/v -
    NA
    440 μl of Polyethylene glycol 3,350 (50 w/v)
    40 μl of Ammonium formate (2.5 M)
    520 μl of water (100 v/v)
    D9
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 3,350 24 w/v -
    pH 5
    480 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    420 μl of water (100 v/v)
    D10
    • Ammonium acetate 0.2 M -
    • Polyethylene glycol 3,350 30 w/v -
    • Sodium acetate 0.1 M pH 4.6
    pH 4.6
    600 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    40 μl of Ammonium acetate (5 M)
    260 μl of water (100 v/v)
    D11
    • 2-Propanol 20 v/v -
    • Calcium chloride 0.2 M -
    • Polyethylene glycol 3,350 30 w/v -
    • Ammonium acetate 0.2 M pH 5.6
    pH 5.6
    200 μl of Ammonium acetate pH 5.6 (1 M)
    200 μl of 2-Propanol (100 v/v)
    40 μl of Calcium chloride (5 M)
    375 μl of Polyethylene glycol 3,350 (80 w/v)
    185 μl of water (100 v/v)
    D12
    • Sodium chloride 0.5 M -
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 3,350 32.5 w/v -
    pH 8.5
    650 μl of Polyethylene glycol 3,350 (50 w/v)
    80 μl of Magnesium chloride (2.5 M)
    100 μl of Sodium chloride (5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    70 μl of water (100 v/v)
    E1
    • Magnesium chloride 0.01 M -
    • Dithiothreitol 0.01 M -
    • Polyethylene glycol 4,000 8 w/v -
    • MES 0.05 M pH 6.5
    pH 6.5
    160 μl of Polyethylene glycol 4,000 (50 w/v)
    4 μl of Magnesium chloride (2.5 M)
    50 μl of MES pH 6.5 (1 M)
    100 μl of Dithiothreitol (0.1 M)
    686 μl of water (100 v/v)
    E2
    • Dithiothreitol 0.01 M -
    • PIPES 0.05 M pH 7
    • Polyethylene glycol 4,000 10 w/v -
    pH 7
    50 μl of PIPES pH 7 (1 M)
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Dithiothreitol (0.1 M)
    650 μl of water (100 v/v)
    E3
    • Ethylene glycol 15 v/v -
    • 2-Propanol 10 v/v -
    • Magnesium chloride 0.2 M -
    • Polyethylene glycol 4,000 10 w/v -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    150 μl of Ethylene glycol (100 v/v)
    80 μl of Magnesium chloride (2.5 M)
    100 μl of 2-Propanol (100 v/v)
    370 μl of water (100 v/v)
    E4
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 10 w/v -
    pH 7.5
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    700 μl of water (100 v/v)
    E5
    • 2-Propanol 10 v/v -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 15 w/v -
    pH 7.5
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    100 μl of 2-Propanol (100 v/v)
    500 μl of water (100 v/v)
    E6
    • Sodium chloride 0.2 M -
    • Polyethylene glycol 6,000 1 w/v -
    • Sodium acetate 0.075 M -
    • Polyethylene glycol 4,000 15 w/v -
    • tris chloride BCC 0.075 M pH 8.5
    pH 8.5
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    37 μl of Sodium acetate (2 M)
    20 μl of Polyethylene glycol 6,000 (50 w/v)
    200 μl of Sodium chloride (1 M)
    75 μl of tris chloride BCC pH 8.5 (1 M)
    367 μl of water (100 v/v)
    E7
    • Ammonium sulfate 0.2 M -
    • di-Sodium DL-malate 0.1 M pH 5.5
    • Polyethylene glycol 4,000 15 w/v -
    pH 5.5
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    31 μl of di-Sodium DL-malate pH 7 (3 M)
    200 μl of Ammonium sulfate (1 M)
    1 μl of di-Sodium DL-malate pH 5 (3 M)
    466 μl of water (100 v/v)
    E8
    • Sodium chloride 0.1 M -
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 4,000 20 w/v -
    pH 6.5
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    100 μl of Sodium chloride (1 M)
    400 μl of water (100 v/v)
    E9
    • Magnesium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 20 w/v -
    pH 7.5
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    80 μl of Magnesium chloride (2.5 M)
    420 μl of water (100 v/v)
    E10
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 20 w/v -
    pH 8.5
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    80 μl of Magnesium chloride (2.5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    420 μl of water (100 v/v)
    E11
    • Magnesium chloride 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 4,000 25 w/v -
    pH 6.5
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    80 μl of Magnesium chloride (2.5 M)
    100 μl of MES pH 6.5 (1 M)
    320 μl of water (100 v/v)
    E12
    • Lithium sulfate 0.1 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 25 w/v -
    pH 8.5
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    33 μl of Lithium sulfate (3 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    366 μl of water (100 v/v)
    F1
    • Lithium acetate 0.2 M pH 7.5
    • Polyethylene glycol 4,000 28 w/v -
    pH 7.5
    200 μl of Lithium acetate pH 7.5 (1 M)
    560 μl of Polyethylene glycol 4,000 (50 w/v)
    240 μl of water (100 v/v)
    F2
    • Sodium acetate 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 4,000 30 w/v -
    pH 6.5
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate (2 M)
    100 μl of MES pH 6.5 (1 M)
    200 μl of water (100 v/v)
    F3
    • Ammonium sulfate 0.2 M -
    • tris chloride BCC 0.15 M pH 8.5
    • Polyethylene glycol 4,000 30 w/v -
    pH 8.5
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    66 μl of Ammonium sulfate (3 M)
    150 μl of tris chloride BCC pH 8.5 (1 M)
    183 μl of water (100 v/v)
    F4
    • 2-Propanol 10 v/v -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol monomethyl ether 5,000 8 w/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    160 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    100 μl of 2-Propanol (100 v/v)
    640 μl of water (100 v/v)
    F5
    • Ammonium sulfate 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 5,000 25 w/v -
    pH 6.5
    500 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    100 μl of MES pH 6.5 (1 M)
    66 μl of Ammonium sulfate (3 M)
    333 μl of water (100 v/v)
    F6
    • Ammonium sulfate 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol monomethyl ether 5,000 30 w/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    600 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    66 μl of Ammonium sulfate (3 M)
    233 μl of water (100 v/v)
    F7
    • Ammonium sulfate 0.1 M -
    • ADA 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 5,000 30 w/v -
    pH 6.5
    600 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    100 μl of ADA pH 6.5 (1 M)
    33 μl of Ammonium sulfate (3 M)
    266 μl of water (100 v/v)
    F8
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 6,000 20 w/v -
    pH 6.5
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of MES pH 6.5 (1 M)
    500 μl of water (100 v/v)
    F9
    • Dithiothreitol 0.01 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 6,000 28 w/v -
    pH 6.5
    560 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of MES pH 6.5 (1 M)
    100 μl of Dithiothreitol (0.1 M)
    240 μl of water (100 v/v)
    F10
    • Lithium sulfate 0.175 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 6,000 30 w/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    600 μl of Polyethylene glycol 6,000 (50 w/v)
    58 μl of Lithium sulfate (3 M)
    241 μl of water (100 v/v)
    F11
    • Dithiothreitol 0.01 M -
    • PIPES 0.1 M pH 7
    • Polyethylene glycol 6,000 30 w/v -
    pH 7
    100 μl of PIPES pH 7 (1 M)
    600 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of Dithiothreitol (0.1 M)
    200 μl of water (100 v/v)
    F12
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 6,000 32 w/v -
    pH 6.5
    640 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of MES pH 6.5 (1 M)
    260 μl of water (100 v/v)
    G1
    • Ethylene glycol 20 v/v -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 8,000 7 w/v -
    pH 6.5
    200 μl of Ethylene glycol (100 v/v)
    140 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of MES pH 6.5 (1 M)
    560 μl of water (100 v/v)
    G2
    • Calcium acetate 0.15 M -
    • Ethylene glycol 16 v/v -
    • Polyethylene glycol 8,000 7 w/v -
    • MES 0.1 M pH 6.5
    pH 6.5
    75 μl of Calcium acetate (2 M)
    160 μl of Ethylene glycol (100 v/v)
    140 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of MES pH 6.5 (1 M)
    525 μl of water (100 v/v)
    G3
    • Polyethylene glycol 200 10 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 8,000 10 w/v -
    pH 8.5
    100 μl of Polyethylene glycol 200 (100 v/v)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    600 μl of water (100 v/v)
    G4
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 8,000 12 w/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    240 μl of Polyethylene glycol 8,000 (50 w/v)
    660 μl of water (100 v/v)
    G5
    • di-Sodium tartrate 0.25 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 8,000 12 w/v -
    pH 8.5
    125 μl of di-Sodium tartrate (2 M)
    240 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    535 μl of water (100 v/v)
    G6
    • Potassium di-hydrogen phosphate 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 8,000 16 w/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    50 μl of Potassium di-hydrogen phosphate (2 M)
    320 μl of Polyethylene glycol 8,000 (50 w/v)
    530 μl of water (100 v/v)
    G7
    • Sodium chloride 0.15 M -
    • Ethylene glycol 2 v/v -
    • Polyethylene glycol 6,000 16 w/v -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    40 μl of Ethylene glycol (50 v/v)
    320 μl of Polyethylene glycol 6,000 (50 w/v)
    30 μl of Sodium chloride (5 M)
    510 μl of water (100 v/v)
    G8
    • Magnesium acetate 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 8,000 18 w/v -
    pH 6.5
    360 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of MES pH 6.5 (1 M)
    100 μl of Magnesium acetate (2 M)
    440 μl of water (100 v/v)
    G9
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 8,000 18 w/v -
    pH 6.5
    360 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of MES pH 6.5 (1 M)
    540 μl of water (100 v/v)
    G10
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 8,000 18 w/v -
    pH 8.5
    360 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    540 μl of water (100 v/v)
    G11
    • Magnesium acetate 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 8,000 20 w/v -
    pH 5
    100 μl of tri-Sodium citrate pH 5 (1 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    50 μl of Magnesium acetate (2 M)
    450 μl of water (100 v/v)
    G12
    • Magnesium chloride 0.2 M -
    • Ethylene glycol 2 v/v -
    • Polyethylene glycol 8,000 20 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    20 μl of Ethylene glycol (100 v/v)
    80 μl of Magnesium chloride (2.5 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of water (100 v/v)
    H1
    • Ethylene glycol 2 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 8,000 22 w/v -
    pH 8.5
    20 μl of Ethylene glycol (100 v/v)
    440 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    440 μl of water (100 v/v)
    H2
    • Magnesium chloride 0.05 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 8,000 25 w/v -
    pH 4.6
    20 μl of Magnesium chloride (2.5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    500 μl of Polyethylene glycol 8,000 (50 w/v)
    380 μl of water (100 v/v)
    H3
    • Ammonium sulfate 0.2 M -
    • 1,3-Propanediol 4 v/v -
    • Polyethylene glycol 8,000 30 w/v -
    • MES 0.1 M pH 6.5
    pH 6.5
    80 μl of 1,3-Propanediol (50 v/v)
    600 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of MES pH 6.5 (1 M)
    66 μl of Ammonium sulfate (3 M)
    153 μl of water (100 v/v)
    H4
    • Dithiothreitol 0.01 M -
    • Glycerol 20 v/v -
    • Polyethylene glycol 8,000 30 w/v -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    600 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Glycerol (100 v/v)
    100 μl of Dithiothreitol (0.1 M)
    H5
    • Zinc acetate 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 8,000 9 w/v -
    pH 6.5
    180 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Zinc acetate (2 M)
    100 μl of MES pH 6.5 (1 M)
    620 μl of water (100 v/v)
    H6
    • Ammonium sulfate 0.3 M -
    • Ethylene glycol 5 v/v -
    • Polyethylene glycol 10,000 16 w/v -
    • BIS-TRIS 0.1 M pH 6.5
    pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    320 μl of Polyethylene glycol 10,000 (50 w/v)
    100 μl of Ethylene glycol (50 v/v)
    100 μl of Ammonium sulfate (3 M)
    380 μl of water (100 v/v)
    H7
    • Ethylene glycol 8 v/v -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 10,000 10 w/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    200 μl of Polyethylene glycol 10,000 (50 w/v)
    80 μl of Ethylene glycol (100 v/v)
    620 μl of water (100 v/v)
    H8
    • Dithiothreitol 0.005 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 10,000 15 w/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    300 μl of Polyethylene glycol 10,000 (50 w/v)
    50 μl of Dithiothreitol (0.1 M)
    550 μl of water (100 v/v)
    H9
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 10,000 15 w/v -
    pH 8.5
    300 μl of Polyethylene glycol 10,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    600 μl of water (100 v/v)
    H10
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 20,000 12 w/v -
    pH 6.5
    240 μl of Polyethylene glycol 20,000 (50 w/v)
    100 μl of MES pH 6.5 (1 M)
    660 μl of water (100 v/v)
    H11
    • Ammonium formate 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 20,000 10 w/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    200 μl of Polyethylene glycol 20,000 (50 w/v)
    40 μl of Ammonium formate (2.5 M)
    660 μl of water (100 v/v)
    H12
    • Potassium hydrogen tartrate 0.01 M -
    • Polyethylene glycol 20,000 15 w/v -
    NA
    100 μl of Potassium hydrogen tartrate (0.1 M)
    300 μl of Polyethylene glycol 20,000 (50 w/v)
    600 μl of water (100 v/v)