C6 - Screen Contents

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Supplemental Information

Screen Description

Kinase HTS

Kinase / Kinase HTS

Created 2009-11-16.

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Kinase HTS

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Kinase HTS

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Screen Structure

Kinase HTS

Well Count	96
Number of distinct ingredients	64
Internal diversity (score mean dist)	0.936

Screen Composition

More information about screen composition chart.

ingredient type	Kinase HTS
Buffer	111
Cryoprotectant	3
Organic (non-volatile)	20
Organic (volatile)	6
Polymer	77
Salt	101

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Kinase HTS Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Magnesium acetate 0.2 M - Dithiothreitol 0.01 M - Ammonium sulfate 1 M - tri-Sodium citrate 0.1 M pH 5.6 🔍	pH 5.6	333.3 μl of Ammonium sulfate (3 M) 100.0 μl of Dithiothreitol (0.1 M) 100.0 μl of Magnesium acetate (2 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 366.7 μl of water (100 v/v)
A2	tri-Sodium citrate 0.1 M pH 5.6 Ammonium sulfate 1.3 M - 🔍	pH 5.6	433.3 μl of Ammonium sulfate (3 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 466.7 μl of water (100 v/v)
A3	tris chloride BCC 0.1 M pH 8.5 Ammonium sulfate 1.3 M - 🔍	pH 8.5	433.3 μl of Ammonium sulfate (3 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 466.7 μl of water (100 v/v)
A4	Cobalt (II) chloride 0.025 M - MES 0.1 M pH 6.5 Ammonium sulfate 1.8 M - 🔍	pH 6.5	600.0 μl of Ammonium sulfate (3 M) 25.0 μl of Cobalt (II) chloride (1 M) 100.0 μl of MES pH 6.5 (1 M) 275.0 μl of water (100 v/v)
A5	Sodium chloride 0.2 M - tri-Sodium citrate 0.1 M pH 3.1 Ammonium sulfate 2 M - 🔍	pH 3.1	666.7 μl of Ammonium sulfate (3 M) 200.0 μl of Sodium chloride (1 M) 100.0 μl of tri-Sodium citrate pH 3.1 (1 M) 33.3 μl of water (100 v/v)
A6	Magnesium chloride 0.05 M - Sodium acetate 0.1 M pH 4.6 Ammonium sulfate 2 M - 🔍	pH 4.6	666.7 μl of Ammonium sulfate (3 M) 20.0 μl of Magnesium chloride (2.5 M) 100.0 μl of Sodium acetate pH 4.6 (1 M) 213.3 μl of water (100 v/v)
A7	Sodium acetate 0.1 M pH 4.6 Ammonium sulfate 2 M - 🔍	pH 4.6	666.7 μl of Ammonium sulfate (3 M) 100.0 μl of Sodium acetate pH 4.6 (1 M) 233.3 μl of water (100 v/v)
A8	Polyethylene glycol monomethyl ether 550 2 v/v - HEPES 0.1 M pH 7.5 Ammonium sulfate 2 M - 🔍	pH 7.5	666.7 μl of Ammonium sulfate (3 M) 100.0 μl of HEPES pH 7.5 (1 M) 20.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 213.3 μl of water (100 v/v)
A9	HEPES 0.1 M pH 7.5 Ammonium sulfate 2 M - 🔍	pH 7.5	666.7 μl of Ammonium sulfate (3 M) 100.0 μl of HEPES pH 7.5 (1 M) 233.3 μl of water (100 v/v)
A10	Magnesium chloride 0.006 M - tris chloride BCC 0.1 M pH 8.5 Ammonium sulfate 2 M - 🔍	pH 8.5	666.7 μl of Ammonium sulfate (3 M) 2.4 μl of Magnesium chloride (2.5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 230.9 μl of water (100 v/v)
A11	Nickel sulfate 0.01 M - tris chloride BCC 0.1 M pH 8.5 Lithium sulfate 1.5 M - 🔍	pH 8.5	500.0 μl of Lithium sulfate (3 M) 2.5 μl of Nickel sulfate (4 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 397.5 μl of water (100 v/v)
A12	tri-Sodium citrate 0.1 M pH 4.2 Lithium chloride 1 M - 🔍	pH 4.2	200.0 μl of Lithium chloride (5 M) 100.0 μl of tri-Sodium citrate pH 4.2 (1 M) 700.0 μl of water (100 v/v)
B1	Sodium acetate 0.1 M pH 4.6 Sodium chloride 2 M - 🔍	pH 4.6	100.0 μl of Sodium acetate pH 4.6 (1 M) 400.0 μl of Sodium chloride (5 M) 500.0 μl of water (100 v/v)
B2	di-Sodium hydrogen phosphate 0.1 M - MES 0.1 M pH 6.5 Sodium chloride 2 M - Potassium di-hydrogen phosphate 0.1 M - 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 50.0 μl of Potassium di-hydrogen phosphate (2 M) 400.0 μl of Sodium chloride (5 M) 20.0 μl of di-Sodium hydrogen phosphate (5 M) 430.0 μl of water (100 v/v)
B3	Glycerol 1 v/v - HEPES 0.1 M pH 7.5 Sodium chloride 3.3 M - 🔍	pH 7.5	10.0 μl of Glycerol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 660.0 μl of Sodium chloride (5 M) 230.0 μl of water (100 v/v)
B4	Calcium chloride 0.0625 M - MES 0.1 M pH 6.5 Sodium acetate 1.2 M - 🔍	pH 6.5	62.5 μl of Calcium chloride (1 M) 100.0 μl of MES pH 6.5 (1 M) 600.0 μl of Sodium acetate (2 M) 237.5 μl of water (100 v/v)
B5	Polyethylene glycol 3,000 2 w/v - BICINE 0.1 M pH 9.5 Sodium formate 3.7 M - 🔍	pH 9.5	100.0 μl of BICINE pH 9.5 (1 M) 40.0 μl of Polyethylene glycol 3,000 (50 w/v) 528.6 μl of Sodium formate (7 M) 331.4 μl of water (100 v/v)
B6	Glycerol 1.6 v/v - Dithiothreitol 0.01 M - di-Sodium malonate 0.5 M - PIPES 0.05 M pH 6 🔍	pH 6	100.0 μl of Dithiothreitol (0.1 M) 16.0 μl of Glycerol (100 v/v) 50.0 μl of PIPES pH 6 (1 M) 166.7 μl of di-Sodium malonate pH 6 (3 M) 667.3 μl of water (100 v/v)
B7	Potassium di-hydrogen phosphate 0.5 M - Lithium sulfate 0.2 M - di-Sodium hydrogen phosphate 0.5 M - CAPS 0.1 M pH 10 🔍	pH 10	100.0 μl of CAPS pH 10 (1 M) 66.7 μl of Lithium sulfate (3 M) 250.0 μl of Potassium di-hydrogen phosphate (2 M) 100.0 μl of di-Sodium hydrogen phosphate (5 M) 483.3 μl of water (100 v/v)
B8	Dithiothreitol 0.005 M - tris chloride BCC 0.1 M pH 8.5 di-Sodium tartrate 1.2 M - 🔍	pH 8.5	50.0 μl of Dithiothreitol (0.1 M) 600.0 μl of di-Sodium tartrate (2 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 250.0 μl of water (100 v/v)
B9	MES 0.1 M pH 6.5 Potassium Sodium tartrate 1 M - 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 500.0 μl of Potassium Sodium tartrate (2 M) 400.0 μl of water (100 v/v)
B10	Cesium chloride 0.05 M - MES 0.1 M pH 6.5 Jeffamine® M-600 30 v/v - 🔍	pH 6.5	50.0 μl of Cesium chloride (1 M) 600.0 μl of Jeffamine® M-600 (50 v/v) 100.0 μl of MES pH 6.5 (1 M) 250.0 μl of water (100 v/v)
B11	MES 0.1 M pH 6.5 2-methyl-2,4-pentanediol BCC 40 v/v - 🔍	pH 6.5	400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of MES pH 6.5 (1 M) 500.0 μl of water (100 v/v)
B12	HEPES 0.1 M pH 7.5 2-methyl-2,4-pentanediol BCC 50 v/v - 🔍	pH 7.5	500.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of water (100 v/v)
C1	Dithiothreitol 0.001 M - Ethylenediaminetetraacetic acid disodium salt 1 mM - Polyethylene glycol 400 10 v/v - tris chloride BCC 0.05 M pH 8.5 Sodium chloride 0.3 M - 🔍	pH 8.5	10.0 μl of Dithiothreitol (0.1 M) 142.9 μl of Ethylenediaminetetraacetic acid disodium salt (7 mM) 100.0 μl of Polyethylene glycol 400 (100 v/v) 60.0 μl of Sodium chloride (5 M) 50.0 μl of tris chloride BCC pH 8.5 (1 M) 637.1 μl of water (100 v/v)
C2	Calcium chloride 0.2 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 400 15 v/v - 🔍	pH 7.5	200.0 μl of Calcium chloride (1 M) 100.0 μl of HEPES pH 7.5 (1 M) 150.0 μl of Polyethylene glycol 400 (100 v/v) 550.0 μl of water (100 v/v)
C3	2-Propanol 10 v/v - MES 0.1 M pH 6.5 Polyethylene glycol 400 25 v/v - 🔍	pH 6.5	100.0 μl of 2-Propanol (100 v/v) 100.0 μl of MES pH 6.5 (1 M) 250.0 μl of Polyethylene glycol 400 (100 v/v) 550.0 μl of water (100 v/v)
C4	tri-Sodium citrate 0.15 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 400 25 v/v - 🔍	pH 8.5	250.0 μl of Polyethylene glycol 400 (100 v/v) 75.0 μl of tri-Sodium citrate (2 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 575.0 μl of water (100 v/v)
C5	Ethylene glycol 5 v/v - Sodium acetate 0.1 M pH 4.6 Polyethylene glycol monomethyl ether 550 15 v/v - 🔍	pH 4.6	100.0 μl of Ethylene glycol (50 v/v) 150.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 650.0 μl of water (100 v/v)
C6	Sodium chloride 0.1 M - BICINE 0.1 M pH 9 Polyethylene glycol monomethyl ether 550 20 v/v - 🔍	pH 9	100.0 μl of BICINE pH 9 (1 M) 200.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 100.0 μl of Sodium chloride (1 M) 600.0 μl of water (100 v/v)
C7	Dithiothreitol 0.001 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 1,000 20 w/v - 🔍	pH 8.5	10.0 μl of Dithiothreitol (0.1 M) 400.0 μl of Polyethylene glycol 1,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 490.0 μl of water (100 v/v)
C8	Lithium sulfate 0.05 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 1,000 35 w/v - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 16.7 μl of Lithium sulfate (3 M) 700.0 μl of Polyethylene glycol 1,000 (50 w/v) 183.3 μl of water (100 v/v)
C9	Magnesium acetate 0.2 M - MES 0.1 M pH 6.5 Polyethylene glycol 2,000 12 w/v - 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 100.0 μl of Magnesium acetate (2 M) 240.0 μl of Polyethylene glycol 2,000 (50 w/v) 560.0 μl of water (100 v/v)
C10	Magnesium chloride 0.1 M - Sodium acetate 0.1 M pH 4.6 Polyethylene glycol 2,000 25 w/v - 🔍	pH 4.6	40.0 μl of Magnesium chloride (2.5 M) 500.0 μl of Polyethylene glycol 2,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 360.0 μl of water (100 v/v)
C11	Magnesium chloride 0.05 M - Sodium acetate 0.1 M pH 4.6 Polyethylene glycol 2,000 30 w/v - 🔍	pH 4.6	20.0 μl of Magnesium chloride (2.5 M) 600.0 μl of Polyethylene glycol 2,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 280.0 μl of water (100 v/v)
C12	Sodium phosphate citrate 0.1 M pH 5 Polyethylene glycol monomethyl ether 2,000 24 w/v - 🔍	pH 5	480.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100.0 μl of Sodium phosphate citrate pH 5 (1 M) 420.0 μl of water (100 v/v)
D1	Sodium chloride 0.5 M - MES 0.1 M pH 6.5 Polyethylene glycol 3,350 12 w/v - 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 240.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of Sodium chloride (5 M) 560.0 μl of water (100 v/v)
D2	Ammonium sulfate 0.2 M - Magnesium sulfate 0.05 M - Polyethylene glycol 3,350 12 w/v - tri-Sodium citrate 0.05 M pH 5.6 🔍	pH 5.6	66.7 μl of Ammonium sulfate (3 M) 50.0 μl of Magnesium sulfate (1 M) 240.0 μl of Polyethylene glycol 3,350 (50 w/v) 50.0 μl of tri-Sodium citrate pH 5.6 (1 M) 593.3 μl of water (100 v/v)
D3	Ammonium sulfate 0.25 M - Cadmium chloride 0.01 M - Polyethylene glycol 3,350 15 w/v - Imidazole 0.1 M pH 7.5 🔍	pH 7.5	83.3 μl of Ammonium sulfate (3 M) 10.0 μl of Cadmium chloride (1 M) 100.0 μl of Imidazole pH 7.5 (1 M) 300.0 μl of Polyethylene glycol 3,350 (50 w/v) 506.7 μl of water (100 v/v)
D4	di-Sodium L-malate 0.15 M pH 7 Polyethylene glycol 3,350 20 w/v - 🔍	pH 7	400.0 μl of Polyethylene glycol 3,350 (50 w/v) 150.0 μl of di-Sodium L-malate pH 7 (1 M) 450.0 μl of water (100 v/v)
D5	Sodium chloride 0.2 M - L-Glutathione reduced 0.02 M - Polyethylene glycol 3,350 20 w/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 100.0 μl of L-Glutathione reduced (0.2 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 40.0 μl of Sodium chloride (5 M) 360.0 μl of water (100 v/v)
D6	Sodium chloride 0.12 M - Dithiothreitol 0.005 M - Polyethylene glycol 3,350 20 w/v - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	50.0 μl of Dithiothreitol (0.1 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 24.0 μl of Sodium chloride (5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 426.0 μl of water (100 v/v)
D7	Potassium nitrate 0.2 M - Polyethylene glycol 3,350 20 w/v - 🔍	NA	400.0 μl of Polyethylene glycol 3,350 (50 w/v) 200.0 μl of Potassium nitrate (1 M) 400.0 μl of water (100 v/v)
D8	Ammonium formate 0.1 M - Polyethylene glycol 3,350 22 w/v - 🔍	NA	40.0 μl of Ammonium formate (2.5 M) 440.0 μl of Polyethylene glycol 3,350 (50 w/v) 520.0 μl of water (100 v/v)
D9	tri-Sodium citrate 0.1 M pH 5 Polyethylene glycol 3,350 24 w/v - 🔍	pH 5	480.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 420.0 μl of water (100 v/v)
D10	Ammonium acetate 0.2 M - Polyethylene glycol 3,350 30 w/v - Sodium acetate 0.1 M pH 4.6 🔍	pH 4.6	40.0 μl of Ammonium acetate (5 M) 600.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 260.0 μl of water (100 v/v)
D11	2-Propanol 20 v/v - Calcium chloride 0.2 M - Polyethylene glycol 3,350 30 w/v - Ammonium acetate 0.2 M pH 5.6 🔍	pH 5.6	200.0 μl of 2-Propanol (100 v/v) 200.0 μl of Ammonium acetate pH 5.6 (1 M) 40.0 μl of Calcium chloride (5 M) 375.0 μl of Polyethylene glycol 3,350 (80 w/v) 185.0 μl of water (100 v/v)
D12	Sodium chloride 0.5 M - Magnesium chloride 0.2 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 3,350 32.5 w/v - 🔍	pH 8.5	80.0 μl of Magnesium chloride (2.5 M) 650.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of Sodium chloride (5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 70.0 μl of water (100 v/v)
E1	Magnesium chloride 0.01 M - Dithiothreitol 0.01 M - Polyethylene glycol 4,000 8 w/v - MES 0.05 M pH 6.5 🔍	pH 6.5	100.0 μl of Dithiothreitol (0.1 M) 50.0 μl of MES pH 6.5 (1 M) 4.0 μl of Magnesium chloride (2.5 M) 160.0 μl of Polyethylene glycol 4,000 (50 w/v) 686.0 μl of water (100 v/v)
E2	Dithiothreitol 0.01 M - PIPES 0.05 M pH 7 Polyethylene glycol 4,000 10 w/v - 🔍	pH 7	100.0 μl of Dithiothreitol (0.1 M) 50.0 μl of PIPES pH 7 (1 M) 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 650.0 μl of water (100 v/v)
E3	Ethylene glycol 15 v/v - 2-Propanol 10 v/v - Magnesium chloride 0.2 M - Polyethylene glycol 4,000 10 w/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100.0 μl of 2-Propanol (100 v/v) 150.0 μl of Ethylene glycol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 80.0 μl of Magnesium chloride (2.5 M) 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 370.0 μl of water (100 v/v)
E4	HEPES 0.1 M pH 7.5 Polyethylene glycol 4,000 10 w/v - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 700.0 μl of water (100 v/v)
E5	2-Propanol 10 v/v - HEPES 0.1 M pH 7.5 Polyethylene glycol 4,000 15 w/v - 🔍	pH 7.5	100.0 μl of 2-Propanol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 300.0 μl of Polyethylene glycol 4,000 (50 w/v) 500.0 μl of water (100 v/v)
E6	Sodium chloride 0.2 M - Polyethylene glycol 6,000 1 w/v - Sodium acetate 0.075 M - Polyethylene glycol 4,000 15 w/v - tris chloride BCC 0.075 M pH 8.5 🔍	pH 8.5	300.0 μl of Polyethylene glycol 4,000 (50 w/v) 20.0 μl of Polyethylene glycol 6,000 (50 w/v) 37.5 μl of Sodium acetate (2 M) 200.0 μl of Sodium chloride (1 M) 75.0 μl of tris chloride BCC pH 8.5 (1 M) 367.5 μl of water (100 v/v)
E7	Ammonium sulfate 0.2 M - di-Sodium DL-malate 0.1 M pH 5.5 Polyethylene glycol 4,000 15 w/v - 🔍	pH 5.5	200.0 μl of Ammonium sulfate (1 M) 300.0 μl of Polyethylene glycol 4,000 (50 w/v) 11.5 μl of di-Sodium DL-malate pH 5 (3 M) 21.9 μl of di-Sodium DL-malate pH 7 (3 M) 466.7 μl of water (100 v/v)
E8	Sodium chloride 0.1 M - BIS-TRIS 0.1 M pH 6.5 Polyethylene glycol 4,000 20 w/v - 🔍	pH 6.5	100.0 μl of BIS-TRIS pH 6.5 (1 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium chloride (1 M) 400.0 μl of water (100 v/v)
E9	Magnesium chloride 0.2 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 4,000 20 w/v - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 80.0 μl of Magnesium chloride (2.5 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 420.0 μl of water (100 v/v)
E10	Magnesium chloride 0.2 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 4,000 20 w/v - 🔍	pH 8.5	80.0 μl of Magnesium chloride (2.5 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 420.0 μl of water (100 v/v)
E11	Magnesium chloride 0.2 M - MES 0.1 M pH 6.5 Polyethylene glycol 4,000 25 w/v - 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 80.0 μl of Magnesium chloride (2.5 M) 500.0 μl of Polyethylene glycol 4,000 (50 w/v) 320.0 μl of water (100 v/v)
E12	Lithium sulfate 0.1 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 4,000 25 w/v - 🔍	pH 8.5	33.3 μl of Lithium sulfate (3 M) 500.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 366.7 μl of water (100 v/v)
F1	Lithium acetate 0.2 M pH 7.5 Polyethylene glycol 4,000 28 w/v - 🔍	pH 7.5	200.0 μl of Lithium acetate pH 7.5 (1 M) 560.0 μl of Polyethylene glycol 4,000 (50 w/v) 240.0 μl of water (100 v/v)
F2	Sodium acetate 0.2 M - MES 0.1 M pH 6.5 Polyethylene glycol 4,000 30 w/v - 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium acetate (2 M) 200.0 μl of water (100 v/v)
F3	Ammonium sulfate 0.2 M - tris chloride BCC 0.15 M pH 8.5 Polyethylene glycol 4,000 30 w/v - 🔍	pH 8.5	66.7 μl of Ammonium sulfate (3 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 150.0 μl of tris chloride BCC pH 8.5 (1 M) 183.3 μl of water (100 v/v)
F4	2-Propanol 10 v/v - HEPES 0.1 M pH 7.5 Polyethylene glycol monomethyl ether 5,000 8 w/v - 🔍	pH 7.5	100.0 μl of 2-Propanol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 160.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 640.0 μl of water (100 v/v)
F5	Ammonium sulfate 0.2 M - MES 0.1 M pH 6.5 Polyethylene glycol monomethyl ether 5,000 25 w/v - 🔍	pH 6.5	66.7 μl of Ammonium sulfate (3 M) 100.0 μl of MES pH 6.5 (1 M) 500.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 333.3 μl of water (100 v/v)
F6	Ammonium sulfate 0.2 M - HEPES 0.1 M pH 7.5 Polyethylene glycol monomethyl ether 5,000 30 w/v - 🔍	pH 7.5	66.7 μl of Ammonium sulfate (3 M) 100.0 μl of HEPES pH 7.5 (1 M) 600.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 233.3 μl of water (100 v/v)
F7	Ammonium sulfate 0.1 M - ADA 0.1 M pH 6.5 Polyethylene glycol monomethyl ether 5,000 30 w/v - 🔍	pH 6.5	100.0 μl of ADA pH 6.5 (1 M) 33.3 μl of Ammonium sulfate (3 M) 600.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 266.7 μl of water (100 v/v)
F8	MES 0.1 M pH 6.5 Polyethylene glycol 6,000 20 w/v - 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 500.0 μl of water (100 v/v)
F9	Dithiothreitol 0.01 M - MES 0.1 M pH 6.5 Polyethylene glycol 6,000 28 w/v - 🔍	pH 6.5	100.0 μl of Dithiothreitol (0.1 M) 100.0 μl of MES pH 6.5 (1 M) 560.0 μl of Polyethylene glycol 6,000 (50 w/v) 240.0 μl of water (100 v/v)
F10	Lithium sulfate 0.175 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 6,000 30 w/v - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 58.3 μl of Lithium sulfate (3 M) 600.0 μl of Polyethylene glycol 6,000 (50 w/v) 241.7 μl of water (100 v/v)
F11	Dithiothreitol 0.01 M - PIPES 0.1 M pH 7 Polyethylene glycol 6,000 30 w/v - 🔍	pH 7	100.0 μl of Dithiothreitol (0.1 M) 100.0 μl of PIPES pH 7 (1 M) 600.0 μl of Polyethylene glycol 6,000 (50 w/v) 200.0 μl of water (100 v/v)
F12	MES 0.1 M pH 6.5 Polyethylene glycol 6,000 32 w/v - 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 640.0 μl of Polyethylene glycol 6,000 (50 w/v) 260.0 μl of water (100 v/v)
G1	Ethylene glycol 20 v/v - MES 0.1 M pH 6.5 Polyethylene glycol 8,000 7 w/v - 🔍	pH 6.5	200.0 μl of Ethylene glycol (100 v/v) 100.0 μl of MES pH 6.5 (1 M) 140.0 μl of Polyethylene glycol 8,000 (50 w/v) 560.0 μl of water (100 v/v)
G2	Calcium acetate 0.15 M - Ethylene glycol 16 v/v - Polyethylene glycol 8,000 7 w/v - MES 0.1 M pH 6.5 🔍	pH 6.5	75.0 μl of Calcium acetate (2 M) 160.0 μl of Ethylene glycol (100 v/v) 100.0 μl of MES pH 6.5 (1 M) 140.0 μl of Polyethylene glycol 8,000 (50 w/v) 525.0 μl of water (100 v/v)
G3	Polyethylene glycol 200 10 v/v - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 8,000 10 w/v - 🔍	pH 8.5	100.0 μl of Polyethylene glycol 200 (100 v/v) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 600.0 μl of water (100 v/v)
G4	HEPES 0.1 M pH 7.5 Polyethylene glycol 8,000 12 w/v - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 240.0 μl of Polyethylene glycol 8,000 (50 w/v) 660.0 μl of water (100 v/v)
G5	di-Sodium tartrate 0.25 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 8,000 12 w/v - 🔍	pH 8.5	240.0 μl of Polyethylene glycol 8,000 (50 w/v) 125.0 μl of di-Sodium tartrate (2 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 535.0 μl of water (100 v/v)
G6	Potassium di-hydrogen phosphate 0.1 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 8,000 16 w/v - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 320.0 μl of Polyethylene glycol 8,000 (50 w/v) 50.0 μl of Potassium di-hydrogen phosphate (2 M) 530.0 μl of water (100 v/v)
G7	Sodium chloride 0.15 M - Ethylene glycol 2 v/v - Polyethylene glycol 6,000 16 w/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	40.0 μl of Ethylene glycol (50 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 320.0 μl of Polyethylene glycol 6,000 (50 w/v) 30.0 μl of Sodium chloride (5 M) 510.0 μl of water (100 v/v)
G8	Magnesium acetate 0.2 M - MES 0.1 M pH 6.5 Polyethylene glycol 8,000 18 w/v - 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 100.0 μl of Magnesium acetate (2 M) 360.0 μl of Polyethylene glycol 8,000 (50 w/v) 440.0 μl of water (100 v/v)
G9	MES 0.1 M pH 6.5 Polyethylene glycol 8,000 18 w/v - 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 360.0 μl of Polyethylene glycol 8,000 (50 w/v) 540.0 μl of water (100 v/v)
G10	tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 8,000 18 w/v - 🔍	pH 8.5	360.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 540.0 μl of water (100 v/v)
G11	Magnesium acetate 0.1 M - tri-Sodium citrate 0.1 M pH 5 Polyethylene glycol 8,000 20 w/v - 🔍	pH 5	50.0 μl of Magnesium acetate (2 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 450.0 μl of water (100 v/v)
G12	Magnesium chloride 0.2 M - Ethylene glycol 2 v/v - Polyethylene glycol 8,000 20 w/v - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	20.0 μl of Ethylene glycol (100 v/v) 80.0 μl of Magnesium chloride (2.5 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 400.0 μl of water (100 v/v)
H1	Ethylene glycol 2 v/v - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 8,000 22 w/v - 🔍	pH 8.5	20.0 μl of Ethylene glycol (100 v/v) 440.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 440.0 μl of water (100 v/v)
H2	Magnesium chloride 0.05 M - Sodium acetate 0.1 M pH 4.6 Polyethylene glycol 8,000 25 w/v - 🔍	pH 4.6	20.0 μl of Magnesium chloride (2.5 M) 500.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 380.0 μl of water (100 v/v)
H3	Ammonium sulfate 0.2 M - 1,3-Propanediol 4 v/v - Polyethylene glycol 8,000 30 w/v - MES 0.1 M pH 6.5 🔍	pH 6.5	80.0 μl of 1,3-Propanediol (50 v/v) 66.7 μl of Ammonium sulfate (3 M) 100.0 μl of MES pH 6.5 (1 M) 600.0 μl of Polyethylene glycol 8,000 (50 w/v) 153.3 μl of water (100 v/v)
H4	Dithiothreitol 0.01 M - Glycerol 20 v/v - Polyethylene glycol 8,000 30 w/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100.0 μl of Dithiothreitol (0.1 M) 200.0 μl of Glycerol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 600.0 μl of Polyethylene glycol 8,000 (50 w/v)
H5	Zinc acetate 0.2 M - MES 0.1 M pH 6.5 Polyethylene glycol 8,000 9 w/v - 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 180.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Zinc acetate (2 M) 620.0 μl of water (100 v/v)
H6	Ammonium sulfate 0.3 M - Ethylene glycol 5 v/v - Polyethylene glycol 10,000 16 w/v - BIS-TRIS 0.1 M pH 6.5 🔍	pH 6.5	100.0 μl of Ammonium sulfate (3 M) 100.0 μl of BIS-TRIS pH 6.5 (1 M) 100.0 μl of Ethylene glycol (50 v/v) 320.0 μl of Polyethylene glycol 10,000 (50 w/v) 380.0 μl of water (100 v/v)
H7	Ethylene glycol 8 v/v - HEPES 0.1 M pH 7.5 Polyethylene glycol 10,000 10 w/v - 🔍	pH 7.5	80.0 μl of Ethylene glycol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 10,000 (50 w/v) 620.0 μl of water (100 v/v)
H8	Dithiothreitol 0.005 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 10,000 15 w/v - 🔍	pH 7.5	50.0 μl of Dithiothreitol (0.1 M) 100.0 μl of HEPES pH 7.5 (1 M) 300.0 μl of Polyethylene glycol 10,000 (50 w/v) 550.0 μl of water (100 v/v)
H9	tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 10,000 15 w/v - 🔍	pH 8.5	300.0 μl of Polyethylene glycol 10,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 600.0 μl of water (100 v/v)
H10	MES 0.1 M pH 6.5 Polyethylene glycol 20,000 12 w/v - 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 240.0 μl of Polyethylene glycol 20,000 (50 w/v) 660.0 μl of water (100 v/v)
H11	Ammonium formate 0.1 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 20,000 10 w/v - 🔍	pH 7.5	40.0 μl of Ammonium formate (2.5 M) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 20,000 (50 w/v) 660.0 μl of water (100 v/v)
H12	Potassium hydrogen tartrate 0.01 M - Polyethylene glycol 20,000 15 w/v - 🔍	NA	300.0 μl of Polyethylene glycol 20,000 (50 w/v) 100.0 μl of Potassium hydrogen tartrate (0.1 M) 600.0 μl of water (100 v/v)