C6 - Screen Contents

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Supplemental Information

Screen Description

Nuc-Pro 1

Nuc-Pro / Nuc-Pro 1

Created 2009-11-16.

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Nuc-Pro 1

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Nuc-Pro 1

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Screen Structure

Nuc-Pro 1

Well Count	24
Number of distinct ingredients	29
Internal diversity (score mean dist)	0.906

Screen Composition

More information about screen composition chart.

ingredient type	Nuc-Pro 1
Buffer	32
Cryoprotectant	2
Organic (non-volatile)	11
Polymer	24
Salt	38

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Nuc-Pro 1 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Polyethylene glycol 200 20 v/v - HEPES 0.05 M pH 7.5 Potassium chloride 0.2 M - Magnesium sulfate 0.025 M - 🔍	pH 7.5	50.0 μl of HEPES pH 7.5 (1 M) 25.0 μl of Magnesium sulfate (1 M) 200.0 μl of Polyethylene glycol 200 (100 v/v) 40.0 μl of Potassium chloride (5 M) 685.0 μl of water (100 v/v)
A2	Polyethylene glycol 200 50 v/v - tris chloride BCC 0.1 M pH 8 🔍	pH 8	500.0 μl of Polyethylene glycol 200 (100 v/v) 100.0 μl of tris chloride BCC pH 8 (1 M) 400.0 μl of water (100 v/v)
A3	Polyethylene glycol 400 5 v/v - PIPES 0.05 M pH 7 Magnesium chloride 0.03 M - 🔍	pH 7	12.0 μl of Magnesium chloride (2.5 M) 50.0 μl of PIPES pH 7 (1 M) 100.0 μl of Polyethylene glycol 400 (50 v/v) 838.0 μl of water (100 v/v)
A4	Polyethylene glycol 400 5 v/v - MES 0.02 M pH 5.8 Magnesium formate 0.015 M - Cobalt (II) chloride 0.002 M - 🔍	pH 5.8	2.0 μl of Cobalt (II) chloride (1 M) 20.0 μl of MES pH 5.8 (1 M) 75.0 μl of Magnesium formate (0.2 M) 100.0 μl of Polyethylene glycol 400 (50 v/v) 803.0 μl of water (100 v/v)
A5	Polyethylene glycol 400 10 v/v - HEPES 0.05 M pH 7 Potassium chloride 0.1 M - 🔍	pH 7	50.0 μl of HEPES pH 7 (1 M) 200.0 μl of Polyethylene glycol 400 (50 v/v) 20.0 μl of Potassium chloride (5 M) 730.0 μl of water (100 v/v)
A6	Polyethylene glycol 400 15 v/v - MES 0.05 M pH 6.5 Magnesium acetate 0.08 M - Magnesium chloride 0.015 M - 🔍	pH 6.5	50.0 μl of MES pH 6.5 (1 M) 80.0 μl of Magnesium acetate (1 M) 6.0 μl of Magnesium chloride (2.5 M) 300.0 μl of Polyethylene glycol 400 (50 v/v) 564.0 μl of water (100 v/v)
B1	Polyethylene glycol 400 15 v/v - tris chloride BCC 0.1 M pH 8 Calcium chloride 0.08 M - Sodium chloride 0.02 M - 🔍	pH 8	80.0 μl of Calcium chloride (1 M) 300.0 μl of Polyethylene glycol 400 (50 v/v) 20.0 μl of Sodium chloride (1 M) 100.0 μl of tris chloride BCC pH 8 (1 M) 500.0 μl of water (100 v/v)
B2	Polyethylene glycol 400 20 v/v - BIS-TRIS propane 0.05 M pH 6.8 Magnesium chloride 0.06 M - 🔍	pH 6.8	50.0 μl of BIS-TRIS propane pH 6.8 (1 M) 24.0 μl of Magnesium chloride (2.5 M) 400.0 μl of Polyethylene glycol 400 (50 v/v) 526.0 μl of water (100 v/v)
B3	Polyethylene glycol 400 25 v/v - tri-Sodium citrate 0.1 M pH 5.6 Sodium chloride 0.13 M - Magnesium chloride 0.06 M - 🔍	pH 5.6	24.0 μl of Magnesium chloride (2.5 M) 500.0 μl of Polyethylene glycol 400 (50 v/v) 130.0 μl of Sodium chloride (1 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 246.0 μl of water (100 v/v)
B4	Polyethylene glycol 400 30 v/v - HEPES 0.1 M pH 7.5 Calcium chloride 0.2 M - 🔍	pH 7.5	40.0 μl of Calcium chloride (5 M) 100.0 μl of HEPES pH 7.5 (1 M) 600.0 μl of Polyethylene glycol 400 (50 v/v) 260.0 μl of water (100 v/v)
B5	Polyethylene glycol 400 30 v/v - tris chloride BCC 0.05 M pH 8.5 Potassium chloride 0.1 M - Magnesium chloride 0.01 M - 🔍	pH 8.5	4.0 μl of Magnesium chloride (2.5 M) 600.0 μl of Polyethylene glycol 400 (50 v/v) 100.0 μl of Potassium chloride (1 M) 50.0 μl of tris chloride BCC pH 8.5 (1 M) 246.0 μl of water (100 v/v)
B6	Polyethylene glycol monomethyl ether 550 25 v/v - HEPES 0.05 M pH 7 Magnesium chloride 0.01 M - 🔍	pH 7	50.0 μl of HEPES pH 7 (1 M) 4.0 μl of Magnesium chloride (2.5 M) 500.0 μl of Polyethylene glycol monomethyl ether 550 (50 v/v) 446.0 μl of water (100 v/v)
C1	Polyethylene glycol 1,000 20 w/v - MES 0.05 M pH 6.5 Magnesium chloride 0.2 M - Sodium chloride 0.1 M - 🔍	pH 6.5	50.0 μl of MES pH 6.5 (1 M) 80.0 μl of Magnesium chloride (2.5 M) 400.0 μl of Polyethylene glycol 1,000 (50 w/v) 100.0 μl of Sodium chloride (1 M) 370.0 μl of water (100 v/v)
C2	Polyethylene glycol monomethyl ether 2,000 30 w/v - Sodium acetate 0.1 M pH 4.6 Ammonium sulfate 0.2 M - 🔍	pH 4.6	200.0 μl of Ammonium sulfate (1 M) 600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 100.0 μl of water (100 v/v)
C3	Polyethylene glycol 3,350 10 w/v - MES 0.1 M pH 6.5 Calcium chloride 0.1 M - Glycerol 13 v/v - 🔍	pH 6.5	100.0 μl of Calcium chloride (1 M) 260.0 μl of Glycerol (50 v/v) 100.0 μl of MES pH 6.5 (1 M) 200.0 μl of Polyethylene glycol 3,350 (50 w/v) 340.0 μl of water (100 v/v)
C4	Polyethylene glycol 3,350 25 w/v - MES 0.05 M pH 6 Sodium formate 0.2 M - Glycerol 10 v/v - 🔍	pH 6	200.0 μl of Glycerol (50 v/v) 50.0 μl of MES pH 6 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 40.0 μl of Sodium formate (5 M) 210.0 μl of water (100 v/v)
C5	Polyethylene glycol 3,350 30 w/v - di-Sodium succinate 0.05 M pH 5.5 Sodium chloride 0.1 M - 🔍	pH 5.5	600.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of Sodium chloride (1 M) 16.7 μl of di-Sodium succinate pH 5.5 (3 M) 283.3 μl of water (100 v/v)
C6	Polyethylene glycol 3,350 35 w/v - tris chloride BCC 0.05 M pH 7.5 Potassium chloride 0.05 M - 🔍	pH 7.5	700.0 μl of Polyethylene glycol 3,350 (50 w/v) 50.0 μl of Potassium chloride (1 M) 50.0 μl of tris chloride BCC pH 7.5 (1 M) 200.0 μl of water (100 v/v)
D1	Polyethylene glycol 4,000 5 w/v - HEPES 0.05 M pH 7 Ammonium sulfate 0.2 M - Magnesium acetate 0.02 M - 🔍	pH 7	200.0 μl of Ammonium sulfate (1 M) 50.0 μl of HEPES pH 7 (1 M) 20.0 μl of Magnesium acetate (1 M) 100.0 μl of Polyethylene glycol 4,000 (50 w/v) 630.0 μl of water (100 v/v)
D2	Polyethylene glycol 4,000 5 w/v - MES 0.05 M pH 6 Magnesium sulfate 0.005 M - 🔍	pH 6	50.0 μl of MES pH 6 (1 M) 5.0 μl of Magnesium sulfate (1 M) 100.0 μl of Polyethylene glycol 4,000 (50 w/v) 845.0 μl of water (100 v/v)
D3	Polyethylene glycol 4,000 10 w/v - MES 0.05 M pH 6.5 Ammonium acetate 0.2 M - Calcium chloride 0.01 M - 🔍	pH 6.5	200.0 μl of Ammonium acetate (1 M) 10.0 μl of Calcium chloride (1 M) 50.0 μl of MES pH 6.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 540.0 μl of water (100 v/v)
D4	Polyethylene glycol 4,000 10 w/v - Imidazole 0.05 M pH 7.2 Zinc sulfate 0.02 M - 🔍	pH 7.2	50.0 μl of Imidazole pH 7.2 (1 M) 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 20.0 μl of Zinc sulfate (1 M) 730.0 μl of water (100 v/v)
D5	Polyethylene glycol 4,000 15 w/v - tris chloride BCC 0.05 M pH 7.5 Potassium chloride 0.15 M - Magnesium chloride 0.02 M - 🔍	pH 7.5	8.0 μl of Magnesium chloride (2.5 M) 300.0 μl of Polyethylene glycol 4,000 (50 w/v) 150.0 μl of Potassium chloride (1 M) 50.0 μl of tris chloride BCC pH 7.5 (1 M) 492.0 μl of water (100 v/v)
D6	Polyethylene glycol 4,000 15 w/v - tri-Sodium citrate 0.05 M pH 5 Sodium chloride 0.1 M - Ammonium sulfate 0.02 M - 🔍	pH 5	20.0 μl of Ammonium sulfate (1 M) 300.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium chloride (1 M) 50.0 μl of tri-Sodium citrate pH 5 (1 M) 530.0 μl of water (100 v/v)