C6 - Screen Contents

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Supplemental Information

Screen Description

Wizard IV

Rigaku / Wizard IV

Created 2009-12-07.

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Wizard IV

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Wizard IV

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Screen Structure

Wizard IV

Well Count	48
Number of distinct ingredients	52
Internal diversity (score mean dist)	0.965

Screen Composition

More information about screen composition chart.

ingredient type	Wizard IV
Buffer	53
Cryoprotectant	1
Organic (non-volatile)	15
Organic (volatile)	6
Polymer	40
Salt	47

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Wizard IV Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Glycerol 20 v/v - di-Potassium hydrogen phosphate 40 mM - Polyethylene glycol 8,000 16 w/v - 🔍	NA	200.0 μl of Glycerol (100 v/v) 320.0 μl of Polyethylene glycol 8,000 (50 w/v) 20.0 μl of di-Potassium hydrogen phosphate (2000 mM) 460.0 μl of water (100 v/v)
A2	Ethanol 15 v/v - tris chloride BCC 0.1 M pH 8 Sodium chloride 100 mM - 2-methyl-2,4-pentanediol BCC 5 v/v - 🔍	pH 8	50.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 150.0 μl of Ethanol (100 v/v) 40.0 μl of Sodium chloride (2500 mM) 100.0 μl of tris chloride BCC pH 8 (1 M) 660.0 μl of water (100 v/v)
A3	Ethanol 40 v/v - Sodium phosphate citrate 0.1 M pH 4.2 Polyethylene glycol 1,000 5 w/v - 🔍	pH 4.2	400.0 μl of Ethanol (100 v/v) 100.0 μl of Polyethylene glycol 1,000 (50 w/v) 100.0 μl of Sodium phosphate citrate pH 4.2 (1 M) 400.0 μl of water (100 v/v)
A4	Ammonium sulfate 200 mM - BIS-TRIS 0.1 M pH 5.5 🔍	pH 5.5	200.0 μl of Ammonium sulfate (1000 mM) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 700.0 μl of water (100 v/v)
A5	Ammonium sulfate 2 M - Sodium acetate 0.1 M pH 5.5 Polyethylene glycol 400 2 v/v - 🔍	pH 5.5	500.0 μl of Ammonium sulfate (4 M) 20.0 μl of Polyethylene glycol 400 (100 v/v) 1.2 μl of Sodium acetate pH 3.6 (3 M) 32.1 μl of Sodium acetate pH 5.6 (3 M) 446.7 μl of water (100 v/v)
A6	Ammonium sulfate 0.8 M - tri-Sodium citrate 0.1 M pH 4 🔍	pH 4	228.6 μl of Ammonium sulfate (3.5 M) 39.4 μl of tri-Sodium citrate pH 3 (1.6 M) 23.1 μl of tri-Sodium citrate pH 6.5 (1.6 M) 708.9 μl of water (100 v/v)
A7	Lithium sulfate 2 M - Sodium acetate 0.1 M pH 4.5 Magnesium sulfate 100 mM - 2-Propanol 5 v/v - 🔍	pH 4.5	50.0 μl of 2-Propanol (100 v/v) 400.0 μl of Lithium sulfate (5 M) 200.0 μl of Magnesium sulfate (500 mM) 33.3 μl of Sodium acetate pH 4.5 (3 M) 316.7 μl of water (100 v/v)
A8	Lithium sulfate 2 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 400 2 v/v - 🔍	pH 8.5	400.0 μl of Lithium sulfate (5 M) 20.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 480.0 μl of water (100 v/v)
B1	Lithium sulfate 2 M - Sodium acetate 0.1 M pH 5.5 Magnesium sulfate 100 mM - Polyethylene glycol 400 5 v/v - 🔍	pH 5.5	400.0 μl of Lithium sulfate (5 M) 200.0 μl of Magnesium sulfate (500 mM) 50.0 μl of Polyethylene glycol 400 (100 v/v) 1.2 μl of Sodium acetate pH 3.6 (3 M) 32.1 μl of Sodium acetate pH 5.6 (3 M) 316.7 μl of water (100 v/v)
B2	Polyethylene glycol 200 50 v/v - Sodium cacodylate 0.1 M pH 6.5 Magnesium chloride 200 mM - 🔍	pH 6.5	400.0 μl of Magnesium chloride (500 mM) 500.0 μl of Polyethylene glycol 200 (100 v/v) 31.1 μl of Sodium cacodylate pH 5 (1 M) 68.9 μl of Sodium cacodylate pH 7.4 (1 M)
B3	Polyethylene glycol 300 40 v/v - Sodium cacodylate 0.1 M pH 6.5 Calcium acetate 200 mM - 🔍	pH 6.5	400.0 μl of Calcium acetate (500 mM) 400.0 μl of Polyethylene glycol 300 (100 v/v) 31.1 μl of Sodium cacodylate pH 5 (1 M) 68.9 μl of Sodium cacodylate pH 7.4 (1 M) 100.0 μl of water (100 v/v)
B4	Jeffamine® M-600 30 v/v - HEPES 0.1 M pH 7 🔍	pH 7	100.0 μl of HEPES pH 7 (1 M) 600.0 μl of Jeffamine® M-600 pH 7 (50 v/v) 300.0 μl of water (100 v/v)
B5	di-Sodium succinate 800 mM pH 7 🔍	pH 7	800.0 μl of di-Sodium succinate pH 7 (1000 mM) 200.0 μl of water (100 v/v)
B6	Polyethylene glycol 400 40 v/v - tris chloride BCC 0.1 M pH 8.5 Lithium sulfate 200 mM - 🔍	pH 8.5	400.0 μl of Lithium sulfate (500 mM) 400.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 100.0 μl of water (100 v/v)
B7	Polyethylene glycol 400 50 v/v - Sodium acetate 0.1 M pH 4.5 Lithium sulfate 200 mM - 🔍	pH 4.5	400.0 μl of Lithium sulfate (500 mM) 500.0 μl of Polyethylene glycol 400 (100 v/v) 33.3 μl of Sodium acetate pH 4.5 (3 M) 66.7 μl of water (100 v/v)
B8	Polyethylene glycol monomethyl ether 550 15 v/v - MES 0.1 M pH 6.5 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 300.0 μl of Polyethylene glycol monomethyl ether 550 (50 v/v) 600.0 μl of water (100 v/v)
C1	Polyethylene glycol 1,500 25 w/v - SPG buffer 0.1 M pH 5.5 🔍	pH 5.5	500.0 μl of Polyethylene glycol 1,500 (50 w/v) 100.0 μl of SPG buffer pH 5.5 (1 M) 400.0 μl of water (100 v/v)
C2	Polyethylene glycol 1,500 25 w/v - SPG buffer 0.1 M pH 8.5 🔍	pH 8.5	500.0 μl of Polyethylene glycol 1,500 (50 w/v) 100.0 μl of SPG buffer pH 8.5 (1 M) 400.0 μl of water (100 v/v)
C3	Polyethylene glycol 1,500 25 w/v - MMT buffer 0.1 M pH 6.5 🔍	pH 6.5	100.0 μl of MMT buffer pH 6.5 (1 M) 500.0 μl of Polyethylene glycol 1,500 (50 w/v) 400.0 μl of water (100 v/v)
C4	Polyethylene glycol 1,500 25 w/v - MMT buffer 0.1 M pH 9 🔍	pH 9	100.0 μl of MMT buffer pH 9 (1 M) 500.0 μl of Polyethylene glycol 1,500 (50 w/v) 400.0 μl of water (100 v/v)
C5	Polyethylene glycol 1,500 25 w/v - MIB buffer 0.1 M pH 5 🔍	pH 5	100.0 μl of MIB buffer pH 5 (1 M) 500.0 μl of Polyethylene glycol 1,500 (50 w/v) 400.0 μl of water (100 v/v)
C6	Polyethylene glycol 1,500 25 w/v - PCB buffer 0.1 M pH 7 🔍	pH 7	100.0 μl of PCB buffer pH 7 (1 M) 500.0 μl of Polyethylene glycol 1,500 (50 w/v) 400.0 μl of water (100 v/v)
C7	Polyethylene glycol 1,500 12 w/v - Sodium acetate 0.1 M pH 5.5 Sodium chloride 2.5 M - 2-methyl-2,4-pentanediol BCC 1.5 v/v - 🔍	pH 5.5	15.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 240.0 μl of Polyethylene glycol 1,500 (50 w/v) 1.2 μl of Sodium acetate pH 3.6 (3 M) 32.1 μl of Sodium acetate pH 5.6 (3 M) 500.0 μl of Sodium chloride (5 M) 211.7 μl of water (100 v/v)
C8	di-Sodium malonate 2.4 M - 🔍	NA	800.0 μl of di-Sodium malonate (3 M) 200.0 μl of water (100 v/v)
D1	Polyethylene glycol monomethyl ether 2,000 30 w/v - Potassium bromide 150 mM - 🔍	NA	600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 300.0 μl of Potassium bromide (500 mM) 100.0 μl of water (100 v/v)
D2	Polyethylene glycol monomethyl ether 2,000 10 w/v - Sodium acetate 0.1 M pH 5.5 Ammonium sulfate 200 mM - 🔍	pH 5.5	200.0 μl of Ammonium sulfate (1000 mM) 200.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 1.2 μl of Sodium acetate pH 3.6 (3 M) 32.1 μl of Sodium acetate pH 5.6 (3 M) 566.7 μl of water (100 v/v)
D3	Polyethylene glycol monomethyl ether 2,000 20 w/v - tris chloride BCC 0.1 M pH 8.5 Trimethylamine N-oxide 200 mM - 🔍	pH 8.5	400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 400.0 μl of Trimethylamine N-oxide (500 mM) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 100.0 μl of water (100 v/v)
D4	Polyethylene glycol 3,350 20 w/v - BIS-TRIS propane 0.1 M pH 6.5 Sodium fluoride 200 mM - 🔍	pH 6.5	100.0 μl of BIS-TRIS propane pH 6.5 (1 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 400.0 μl of Sodium fluoride (500 mM) 100.0 μl of water (100 v/v)
D5	Polyethylene glycol 3,350 20 w/v - tri-Sodium citrate 0.3 M pH 4 🔍	pH 4	400.0 μl of Polyethylene glycol 3,350 (50 w/v) 300.0 μl of tri-Sodium citrate pH 4 (1 M) 300.0 μl of water (100 v/v)
D6	Polyethylene glycol 3,350 20 w/v - BIS-TRIS propane 0.1 M pH 8.5 di-Sodium malonate 200 mM - 🔍	pH 8.5	100.0 μl of BIS-TRIS propane pH 8.5 (1 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 66.7 μl of di-Sodium malonate (3000 mM) 433.3 μl of water (100 v/v)
D7	Poly(acrylic acid sodium salt) 5,100 20 w/v - HEPES 0.1 M pH 7 Magnesium chloride 20 mM - 🔍	pH 7	100.0 μl of HEPES pH 7 (1 M) 40.0 μl of Magnesium chloride (500 mM) 400.0 μl of Poly(acrylic acid sodium salt) 5,100 (50 w/v) 460.0 μl of water (100 v/v)
D8	di-Sodium DL-malate 2.1e+03 mM pH 7 🔍	pH 7	840.0 μl of di-Sodium DL-malate pH 7 (2500 mM) 160.0 μl of water (100 v/v)
E1	di-Potassium hydrogen phosphate 800 mM - HEPES 0.1 M pH 7.5 Sodium di-hydrogen phosphate 800 mM - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Sodium di-hydrogen phosphate (2000 mM) 400.0 μl of di-Potassium hydrogen phosphate (2000 mM) 100.0 μl of water (100 v/v)
E2	Polyethylene glycol 6,000 20 w/v - MES 0.1 M pH 6 Ammonium chloride 200 mM - 🔍	pH 6	400.0 μl of Ammonium chloride (500 mM) 117.0 μl of MES pH 5.2 (0.5 M) 83.0 μl of MES pH 7.1 (0.5 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v)
E3	Polyethylene glycol 6,000 20 w/v - HEPES 0.1 M pH 7 Sodium chloride 200 mM - 🔍	pH 7	100.0 μl of HEPES pH 7 (1 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 50.0 μl of Sodium chloride (4000 mM) 450.0 μl of water (100 v/v)
E4	Polyethylene glycol 6,000 20 w/v - tris chloride BCC 0.1 M pH 8 Lithium chloride 200 mM - 🔍	pH 8	400.0 μl of Lithium chloride (500 mM) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8 (1 M) 100.0 μl of water (100 v/v)
E5	Polyvinylpyrrolidone K15 20 w/v - tris chloride BCC 0.1 M pH 8.5 Cobalt (II) chloride 100 mM - 🔍	pH 8.5	333.3 μl of Cobalt (II) chloride (300 mM) 400.0 μl of Polyvinylpyrrolidone K15 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 166.7 μl of water (100 v/v)
E6	Ethylene glycol 50 v/v - tris chloride BCC 0.1 M pH 8.5 Magnesium chloride 200 mM - 🔍	pH 8.5	500.0 μl of Ethylene glycol (100 v/v) 400.0 μl of Magnesium chloride (500 mM) 100.0 μl of tris chloride BCC pH 8.5 (1 M)
E7	Polyethylene glycol 8,000 20 w/v - Imidazole 0.1 M pH 6.5 2-methyl-2,4-pentanediol BCC 3 v/v - 🔍	pH 6.5	30.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of Imidazole pH 6.5 (1 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 470.0 μl of water (100 v/v)
E8	Polyethylene glycol 8,000 20 w/v - tris chloride BCC 0.1 M pH 8.5 Magnesium chloride 100 mM - Polyethylene glycol 400 20 v/v - 🔍	pH 8.5	200.0 μl of Magnesium chloride (500 mM) 200.0 μl of Polyethylene glycol 400 (100 v/v) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 100.0 μl of water (100 v/v)
F1	Polyethylene glycol 8,000 20 w/v - HEPES 0.1 M pH 7.5 Ammonium sulfate 200 mM - 2-Propanol 10 v/v - 🔍	pH 7.5	100.0 μl of 2-Propanol (100 v/v) 200.0 μl of Ammonium sulfate (1000 mM) 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 200.0 μl of water (100 v/v)
F2	2-methyl-2,4-pentanediol BCC 30 v/v - Sodium acetate 0.1 M pH 4.5 Polyethylene glycol 1,500 25 w/v - 🔍	pH 4.5	300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 500.0 μl of Polyethylene glycol 1,500 (50 w/v) 33.3 μl of Sodium acetate pH 4.5 (3 M) 166.7 μl of water (100 v/v)
F3	2-methyl-2,4-pentanediol BCC 30 v/v - Imidazole 0.1 M pH 6.5 Ammonium sulfate 200 mM - Polyethylene glycol 3,350 10 w/v - 🔍	pH 6.5	300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 200.0 μl of Ammonium sulfate (1000 mM) 100.0 μl of Imidazole pH 6.5 (1 M) 200.0 μl of Polyethylene glycol 3,350 (50 w/v) 200.0 μl of water (100 v/v)
F4	2-methyl-2,4-pentanediol BCC 30 v/v - tris chloride BCC 0.1 M pH 8.5 Sodium chloride 0.5 M - Polyethylene glycol 8,000 8 w/v - 🔍	pH 8.5	300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 160.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Sodium chloride (5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 340.0 μl of water (100 v/v)
F5	2-Propanol 40 v/v - Imidazole 0.1 M pH 6.5 Polyethylene glycol 8,000 15 w/v - 🔍	pH 6.5	400.0 μl of 2-Propanol (100 v/v) 100.0 μl of Imidazole pH 6.5 (1 M) 300.0 μl of Polyethylene glycol 8,000 (50 w/v) 200.0 μl of water (100 v/v)
F6	2-Propanol 30 v/v - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 3,350 30 w/v - 🔍	pH 8.5	300.0 μl of 2-Propanol (100 v/v) 600.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M)
F7	Polyethylene glycol 10,000 17 w/v - BIS-TRIS 0.1 M pH 5.5 Ammonium acetate 100 mM - 🔍	pH 5.5	333.3 μl of Ammonium acetate (300 mM) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 340.0 μl of Polyethylene glycol 10,000 (50 w/v) 226.7 μl of water (100 v/v)
F8	Polyethylene glycol 20,000 15 w/v - HEPES 0.1 M pH 7 🔍	pH 7	100.0 μl of HEPES pH 7 (1 M) 500.0 μl of Polyethylene glycol 20,000 (30 w/v) 400.0 μl of water (100 v/v)