C6 - Screen Contents

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Supplemental Information

Screen Description

Wizard IV

Rigaku / Wizard IV

Created 2009-12-07.

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Wizard IV

Barcodes

Wizard IV

Screen Structure

Wizard IV

Well Count	48
Number of distinct ingredients	52
Internal diversity (score mean dist)	0.965

Screen Composition

More information about screen composition chart.

ingredient type	Wizard IV
Buffer	53
Cryoprotectant	1
Organic (non-volatile)	15
Organic (volatile)	6
Polymer	40
Salt	47

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Wizard IV Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Glycerol 20 v/v - di-Potassium hydrogen phosphate 40 mM - Polyethylene glycol 8,000 16 w/v - 🔍	NA	320 μl of Polyethylene glycol 8,000 (50 w/v) 200 μl of Glycerol (100 v/v) 20 μl of di-Potassium hydrogen phosphate (2000 mM) 460 μl of water (100 v/v)
A2	Ethanol 15 v/v - tris chloride BCC 0.1 M pH 8 Sodium chloride 100 mM - 2-methyl-2,4-pentanediol BCC 5 v/v - 🔍	pH 8	100 μl of tris chloride BCC pH 8 (1 M) 50 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 150 μl of Ethanol (100 v/v) 40 μl of Sodium chloride (2500 mM) 660 μl of water (100 v/v)
A3	Ethanol 40 v/v - Sodium phosphate citrate 0.1 M pH 4.2 Polyethylene glycol 1,000 5 w/v - 🔍	pH 4.2	100 μl of Polyethylene glycol 1,000 (50 w/v) 400 μl of Ethanol (100 v/v) 100 μl of Sodium phosphate citrate pH 4.2 (1 M) 400 μl of water (100 v/v)
A4	Ammonium sulfate 200 mM - BIS-TRIS 0.1 M pH 5.5 🔍	pH 5.5	200 μl of Ammonium sulfate (1000 mM) 100 μl of BIS-TRIS pH 5.5 (1 M) 700 μl of water (100 v/v)
A5	Ammonium sulfate 2 M - Sodium acetate 0.1 M pH 5.5 Polyethylene glycol 400 2 v/v - 🔍	pH 5.5	1 μl of Sodium acetate pH 3.6 (3 M) 32 μl of Sodium acetate pH 5.6 (3 M) 20 μl of Polyethylene glycol 400 (100 v/v) 500 μl of Ammonium sulfate (4 M) 446 μl of water (100 v/v)
A6	Ammonium sulfate 0.8 M - tri-Sodium citrate 0.1 M pH 4 🔍	pH 4	228 μl of Ammonium sulfate (3.5 M) 10 μl of tri-Sodium citrate pH 3 (1.6 M) 51 μl of tri-Sodium citrate pH 6.5 (1.6 M) 708 μl of water (100 v/v)
A7	Lithium sulfate 2 M - Sodium acetate 0.1 M pH 4.5 Magnesium sulfate 100 mM - 2-Propanol 5 v/v - 🔍	pH 4.5	200 μl of Magnesium sulfate (500 mM) 33 μl of Sodium acetate pH 4.5 (3 M) 50 μl of 2-Propanol (100 v/v) 400 μl of Lithium sulfate (5 M) 316 μl of water (100 v/v)
A8	Lithium sulfate 2 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 400 2 v/v - 🔍	pH 8.5	20 μl of Polyethylene glycol 400 (100 v/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 400 μl of Lithium sulfate (5 M) 480 μl of water (100 v/v)
B1	Lithium sulfate 2 M - Sodium acetate 0.1 M pH 5.5 Magnesium sulfate 100 mM - Polyethylene glycol 400 5 v/v - 🔍	pH 5.5	1 μl of Sodium acetate pH 3.6 (3 M) 200 μl of Magnesium sulfate (500 mM) 32 μl of Sodium acetate pH 5.6 (3 M) 50 μl of Polyethylene glycol 400 (100 v/v) 400 μl of Lithium sulfate (5 M) 316 μl of water (100 v/v)
B2	Polyethylene glycol 200 50 v/v - Sodium cacodylate 0.1 M pH 6.5 Magnesium chloride 200 mM - 🔍	pH 6.5	500 μl of Polyethylene glycol 200 (100 v/v) 400 μl of Magnesium chloride (500 mM) 31 μl of Sodium cacodylate pH 5 (1 M) 68 μl of Sodium cacodylate pH 7.4 (1 M)
B3	Polyethylene glycol 300 40 v/v - Sodium cacodylate 0.1 M pH 6.5 Calcium acetate 200 mM - 🔍	pH 6.5	400 μl of Calcium acetate (500 mM) 31 μl of Sodium cacodylate pH 5 (1 M) 400 μl of Polyethylene glycol 300 (100 v/v) 68 μl of Sodium cacodylate pH 7.4 (1 M) 100 μl of water (100 v/v)
B4	Jeffamine® M-600 30 v/v - HEPES 0.1 M pH 7 🔍	pH 7	100 μl of HEPES pH 7 (1 M) 600 μl of Jeffamine® M-600 pH 7 (50 v/v) 300 μl of water (100 v/v)
B5	di-Sodium succinate 800 mM pH 7 🔍	pH 7	800 μl of di-Sodium succinate pH 7 (1000 mM) 200 μl of water (100 v/v)
B6	Polyethylene glycol 400 40 v/v - tris chloride BCC 0.1 M pH 8.5 Lithium sulfate 200 mM - 🔍	pH 8.5	400 μl of Lithium sulfate (500 mM) 400 μl of Polyethylene glycol 400 (100 v/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 100 μl of water (100 v/v)
B7	Polyethylene glycol 400 50 v/v - Sodium acetate 0.1 M pH 4.5 Lithium sulfate 200 mM - 🔍	pH 4.5	33 μl of Sodium acetate pH 4.5 (3 M) 400 μl of Lithium sulfate (500 mM) 500 μl of Polyethylene glycol 400 (100 v/v) 66 μl of water (100 v/v)
B8	Polyethylene glycol monomethyl ether 550 15 v/v - MES 0.1 M pH 6.5 🔍	pH 6.5	100 μl of MES pH 6.5 (1 M) 300 μl of Polyethylene glycol monomethyl ether 550 (50 v/v) 600 μl of water (100 v/v)
C1	Polyethylene glycol 1,500 25 w/v - SPG buffer 0.1 M pH 5.5 🔍	pH 5.5	500 μl of Polyethylene glycol 1,500 (50 w/v) 100 μl of SPG buffer pH 5.5 (1 M) 400 μl of water (100 v/v)
C2	Polyethylene glycol 1,500 25 w/v - SPG buffer 0.1 M pH 8.5 🔍	pH 8.5	100 μl of SPG buffer pH 8.5 (1 M) 500 μl of Polyethylene glycol 1,500 (50 w/v) 400 μl of water (100 v/v)
C3	Polyethylene glycol 1,500 25 w/v - MMT buffer 0.1 M pH 6.5 🔍	pH 6.5	100 μl of MMT buffer pH 6.5 (1 M) 500 μl of Polyethylene glycol 1,500 (50 w/v) 400 μl of water (100 v/v)
C4	Polyethylene glycol 1,500 25 w/v - MMT buffer 0.1 M pH 9 🔍	pH 9	100 μl of MMT buffer pH 9 (1 M) 500 μl of Polyethylene glycol 1,500 (50 w/v) 400 μl of water (100 v/v)
C5	Polyethylene glycol 1,500 25 w/v - MIB buffer 0.1 M pH 5 🔍	pH 5	500 μl of Polyethylene glycol 1,500 (50 w/v) 100 μl of MIB buffer pH 5 (1 M) 400 μl of water (100 v/v)
C6	Polyethylene glycol 1,500 25 w/v - PCB buffer 0.1 M pH 7 🔍	pH 7	100 μl of PCB buffer pH 7 (1 M) 500 μl of Polyethylene glycol 1,500 (50 w/v) 400 μl of water (100 v/v)
C7	Polyethylene glycol 1,500 12 w/v - Sodium acetate 0.1 M pH 5.5 Sodium chloride 2.5 M - 2-methyl-2,4-pentanediol BCC 1.5 v/v - 🔍	pH 5.5	1 μl of Sodium acetate pH 3.6 (3 M) 32 μl of Sodium acetate pH 5.6 (3 M) 15 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 240 μl of Polyethylene glycol 1,500 (50 w/v) 500 μl of Sodium chloride (5 M) 211 μl of water (100 v/v)
C8	di-Sodium malonate 2.4 M - 🔍	NA	800 μl of di-Sodium malonate (3 M) 200 μl of water (100 v/v)
D1	Polyethylene glycol monomethyl ether 2,000 30 w/v - Potassium bromide 150 mM - 🔍	NA	300 μl of Potassium bromide (500 mM) 600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100 μl of water (100 v/v)
D2	Polyethylene glycol monomethyl ether 2,000 10 w/v - Sodium acetate 0.1 M pH 5.5 Ammonium sulfate 200 mM - 🔍	pH 5.5	1 μl of Sodium acetate pH 3.6 (3 M) 32 μl of Sodium acetate pH 5.6 (3 M) 200 μl of Ammonium sulfate (1000 mM) 200 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 566 μl of water (100 v/v)
D3	Polyethylene glycol monomethyl ether 2,000 20 w/v - tris chloride BCC 0.1 M pH 8.5 Trimethylamine N-oxide 200 mM - 🔍	pH 8.5	400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 400 μl of Trimethylamine N-oxide (500 mM) 100 μl of tris chloride BCC pH 8.5 (1 M) 100 μl of water (100 v/v)
D4	Polyethylene glycol 3,350 20 w/v - BIS-TRIS propane 0.1 M pH 6.5 Sodium fluoride 200 mM - 🔍	pH 6.5	100 μl of BIS-TRIS propane pH 6.5 (1 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 400 μl of Sodium fluoride (500 mM) 100 μl of water (100 v/v)
D5	Polyethylene glycol 3,350 20 w/v - tri-Sodium citrate 0.3 M pH 4 🔍	pH 4	400 μl of Polyethylene glycol 3,350 (50 w/v) 300 μl of tri-Sodium citrate pH 4 (1 M) 300 μl of water (100 v/v)
D6	Polyethylene glycol 3,350 20 w/v - BIS-TRIS propane 0.1 M pH 8.5 di-Sodium malonate 200 mM - 🔍	pH 8.5	66 μl of di-Sodium malonate (3000 mM) 400 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of BIS-TRIS propane pH 8.5 (1 M) 433 μl of water (100 v/v)
D7	Poly(acrylic acid sodium salt) 5,100 20 w/v - HEPES 0.1 M pH 7 Magnesium chloride 20 mM - 🔍	pH 7	40 μl of Magnesium chloride (500 mM) 100 μl of HEPES pH 7 (1 M) 400 μl of Poly(acrylic acid sodium salt) 5,100 (50 w/v) 460 μl of water (100 v/v)
D8	di-Sodium DL-malate 2.1e+03 mM pH 7 🔍	pH 7	840 μl of di-Sodium DL-malate pH 7 (2500 mM) 160 μl of water (100 v/v)
E1	di-Potassium hydrogen phosphate 800 mM - HEPES 0.1 M pH 7.5 Sodium di-hydrogen phosphate 800 mM - 🔍	pH 7.5	400 μl of Sodium di-hydrogen phosphate (2000 mM) 100 μl of HEPES pH 7.5 (1 M) 400 μl of di-Potassium hydrogen phosphate (2000 mM) 100 μl of water (100 v/v)
E2	Polyethylene glycol 6,000 20 w/v - MES 0.1 M pH 6 Ammonium chloride 200 mM - 🔍	pH 6	400 μl of Polyethylene glycol 6,000 (50 w/v) 117 μl of MES pH 5.2 (0.5 M) 400 μl of Ammonium chloride (500 mM) 83 μl of MES pH 7.1 (0.5 M)
E3	Polyethylene glycol 6,000 20 w/v - HEPES 0.1 M pH 7 Sodium chloride 200 mM - 🔍	pH 7	50 μl of Sodium chloride (4000 mM) 100 μl of HEPES pH 7 (1 M) 400 μl of Polyethylene glycol 6,000 (50 w/v) 450 μl of water (100 v/v)
E4	Polyethylene glycol 6,000 20 w/v - tris chloride BCC 0.1 M pH 8 Lithium chloride 200 mM - 🔍	pH 8	100 μl of tris chloride BCC pH 8 (1 M) 400 μl of Lithium chloride (500 mM) 400 μl of Polyethylene glycol 6,000 (50 w/v) 100 μl of water (100 v/v)
E5	Polyvinylpyrrolidone K15 20 w/v - tris chloride BCC 0.1 M pH 8.5 Cobalt (II) chloride 100 mM - 🔍	pH 8.5	333 μl of Cobalt (II) chloride (300 mM) 400 μl of Polyvinylpyrrolidone K15 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 166 μl of water (100 v/v)
E6	Ethylene glycol 50 v/v - tris chloride BCC 0.1 M pH 8.5 Magnesium chloride 200 mM - 🔍	pH 8.5	500 μl of Ethylene glycol (100 v/v) 400 μl of Magnesium chloride (500 mM) 100 μl of tris chloride BCC pH 8.5 (1 M)
E7	Polyethylene glycol 8,000 20 w/v - Imidazole 0.1 M pH 6.5 2-methyl-2,4-pentanediol BCC 3 v/v - 🔍	pH 6.5	30 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100 μl of Imidazole pH 6.5 (1 M) 400 μl of Polyethylene glycol 8,000 (50 w/v) 470 μl of water (100 v/v)
E8	Polyethylene glycol 8,000 20 w/v - tris chloride BCC 0.1 M pH 8.5 Magnesium chloride 100 mM - Polyethylene glycol 400 20 v/v - 🔍	pH 8.5	200 μl of Magnesium chloride (500 mM) 400 μl of Polyethylene glycol 8,000 (50 w/v) 200 μl of Polyethylene glycol 400 (100 v/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 100 μl of water (100 v/v)
F1	Polyethylene glycol 8,000 20 w/v - HEPES 0.1 M pH 7.5 Ammonium sulfate 200 mM - 2-Propanol 10 v/v - 🔍	pH 7.5	200 μl of Ammonium sulfate (1000 mM) 100 μl of 2-Propanol (100 v/v) 400 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 200 μl of water (100 v/v)
F2	2-methyl-2,4-pentanediol BCC 30 v/v - Sodium acetate 0.1 M pH 4.5 Polyethylene glycol 1,500 25 w/v - 🔍	pH 4.5	33 μl of Sodium acetate pH 4.5 (3 M) 300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 500 μl of Polyethylene glycol 1,500 (50 w/v) 166 μl of water (100 v/v)
F3	2-methyl-2,4-pentanediol BCC 30 v/v - Imidazole 0.1 M pH 6.5 Ammonium sulfate 200 mM - Polyethylene glycol 3,350 10 w/v - 🔍	pH 6.5	200 μl of Ammonium sulfate (1000 mM) 300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 200 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of Imidazole pH 6.5 (1 M) 200 μl of water (100 v/v)
F4	2-methyl-2,4-pentanediol BCC 30 v/v - tris chloride BCC 0.1 M pH 8.5 Sodium chloride 0.5 M - Polyethylene glycol 8,000 8 w/v - 🔍	pH 8.5	300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 160 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of Sodium chloride (5 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 340 μl of water (100 v/v)
F5	2-Propanol 40 v/v - Imidazole 0.1 M pH 6.5 Polyethylene glycol 8,000 15 w/v - 🔍	pH 6.5	400 μl of 2-Propanol (100 v/v) 100 μl of Imidazole pH 6.5 (1 M) 300 μl of Polyethylene glycol 8,000 (50 w/v) 200 μl of water (100 v/v)
F6	2-Propanol 30 v/v - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 3,350 30 w/v - 🔍	pH 8.5	600 μl of Polyethylene glycol 3,350 (50 w/v) 300 μl of 2-Propanol (100 v/v) 100 μl of tris chloride BCC pH 8.5 (1 M)
F7	Polyethylene glycol 10,000 17 w/v - BIS-TRIS 0.1 M pH 5.5 Ammonium acetate 100 mM - 🔍	pH 5.5	333 μl of Ammonium acetate (300 mM) 100 μl of BIS-TRIS pH 5.5 (1 M) 340 μl of Polyethylene glycol 10,000 (50 w/v) 226 μl of water (100 v/v)
F8	Polyethylene glycol 20,000 15 w/v - HEPES 0.1 M pH 7 🔍	pH 7	100 μl of HEPES pH 7 (1 M) 500 μl of Polyethylene glycol 20,000 (30 w/v) 400 μl of water (100 v/v)