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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
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Structure I
Barcodes
Structure I
Screen Structure
Structure I
Well Count | 96 |
---|---|
Number of distinct ingredients | 28 |
Internal diversity (score mean dist) | 0.938 |
Screen Composition
ingredient type | Structure I |
---|---|
Buffer | 62 |
Organic (non-volatile) | 8 |
Organic (volatile) | 7 |
Polymer | 25 |
Salt | 65 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 4.6 | 64 μl of Sodium acetate pH 4 (1 M) 35 μl of Sodium acetate pH 5.5 (1 M) 10 μl of Calcium chloride (2 M) 300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 590 μl of water (100 v/v) |
A2 |
| pH 4.6 | 64 μl of Sodium acetate pH 4 (1 M) 35 μl of Sodium acetate pH 5.5 (1 M) 200 μl of Ammonium acetate (1 M) 600 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of water (100 v/v) |
A3 |
| pH 4.6 | 57 μl of Ammonium sulfate (3.5 M) 64 μl of Sodium acetate pH 4 (1 M) 35 μl of Sodium acetate pH 5.5 (1 M) 500 μl of Polyethylene glycol 4,000 (50 w/v) 342 μl of water (100 v/v) |
A4 |
| pH 4.6 | 64 μl of Sodium acetate pH 4 (1 M) 35 μl of Sodium acetate pH 5.5 (1 M) 285 μl of Sodium formate (7 M) 614 μl of water (100 v/v) |
A5 |
| pH 4.6 | 571 μl of Ammonium sulfate (3.5 M) 64 μl of Sodium acetate pH 4 (1 M) 35 μl of Sodium acetate pH 5.5 (1 M) 328 μl of water (100 v/v) |
A6 |
| pH 4.6 | 64 μl of Sodium acetate pH 4 (1 M) 35 μl of Sodium acetate pH 5.5 (1 M) 160 μl of Polyethylene glycol 4,000 (50 w/v) 740 μl of water (100 v/v) |
A7 |
| pH 5.6 | 59 μl of tri-Sodium citrate pH 6.5 (1.6 M) 200 μl of Ammonium acetate (1 M) 3 μl of tri-Sodium citrate pH 3 (1.6 M) 600 μl of Polyethylene glycol 4,000 (50 w/v) 137 μl of water (100 v/v) |
A8 |
| pH 5.6 | 59 μl of tri-Sodium citrate pH 6.5 (1.6 M) 200 μl of Ammonium acetate (1 M) 3 μl of tri-Sodium citrate pH 3 (1.6 M) 300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 437 μl of water (100 v/v) |
A9 |
| pH 5.6 | 59 μl of tri-Sodium citrate pH 6.5 (1.6 M) 200 μl of 2-Propanol (100 v/v) 3 μl of tri-Sodium citrate pH 3 (1.6 M) 400 μl of Polyethylene glycol 4,000 (50 w/v) 337 μl of water (100 v/v) |
A10 |
| pH 5.6 | 59 μl of tri-Sodium citrate pH 6.5 (1.6 M) 400 μl of Ammonium di-hydrogen phosphate (2.5 M) 3 μl of tri-Sodium citrate pH 3 (1.6 M) 537 μl of water (100 v/v) |
A11 |
| pH 4.6 | 64 μl of Sodium acetate pH 4 (1 M) 35 μl of Sodium acetate pH 5.5 (1 M) 200 μl of 2-Propanol (100 v/v) 100 μl of Calcium chloride (2 M) 600 μl of water (100 v/v) |
A12 |
| pH 6.5 | 100 μl of Sodium cacodylate pH 6.5 (1 M) 466 μl of Sodium acetate (3 M) 433 μl of water (100 v/v) |
B1 |
| pH 6.5 | 100 μl of Sodium cacodylate pH 6.5 (1 M) 300 μl of 2-Propanol (100 v/v) 125 μl of tri-Sodium citrate (1.6 M) 475 μl of water (100 v/v) |
B2 |
| pH 6.5 | 57 μl of Ammonium sulfate (3.5 M) 100 μl of Sodium cacodylate pH 6.5 (1 M) 600 μl of Polyethylene glycol 8,000 (50 w/v) 242 μl of water (100 v/v) |
B3 |
| pH 6.5 | 100 μl of Sodium cacodylate pH 6.5 (1 M) 200 μl of Magnesium acetate (1 M) 400 μl of Polyethylene glycol 8,000 (50 w/v) 300 μl of water (100 v/v) |
B4 |
| pH 6.5 | 100 μl of Sodium cacodylate pH 6.5 (1 M) 200 μl of Magnesium acetate (1 M) 300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 400 μl of water (100 v/v) |
B5 |
| pH 6.5 | 333 μl of Sodium acetate (3 M) 86 μl of Imidazole pH 6.2 (1 M) 13 μl of Imidazole pH 8 (1 M) 566 μl of water (100 v/v) |
B6 |
| pH 6.5 | 100 μl of Sodium cacodylate pH 6.5 (1 M) 66 μl of Sodium acetate (3 M) 600 μl of Polyethylene glycol 8,000 (50 w/v) 233 μl of water (100 v/v) |
B7 |
| pH 6.5 | 200 μl of Zinc acetate (1 M) 100 μl of Sodium cacodylate pH 6.5 (1 M) 360 μl of Polyethylene glycol 8,000 (50 w/v) 340 μl of water (100 v/v) |
B8 |
| pH 6.5 | 100 μl of Sodium cacodylate pH 6.5 (1 M) 200 μl of Calcium acetate (1 M) 360 μl of Polyethylene glycol 8,000 (50 w/v) 340 μl of water (100 v/v) |
B9 |
| pH 7.5 | 48 μl of HEPES pH 8.5 (1 M) 125 μl of tri-Sodium citrate (1.6 M) 300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 51 μl of HEPES pH 6.6 (1 M) 475 μl of water (100 v/v) |
B10 |
| pH 7.5 | 300 μl of 2-Propanol (100 v/v) 48 μl of HEPES pH 8.5 (1 M) 100 μl of Magnesium chloride (2 M) 51 μl of HEPES pH 6.6 (1 M) 500 μl of water (100 v/v) |
B11 |
| pH 7.5 | 48 μl of HEPES pH 8.5 (1 M) 100 μl of Calcium chloride (2 M) 280 μl of Polyethylene glycol 400 (100 v/v) 51 μl of HEPES pH 6.6 (1 M) 520 μl of water (100 v/v) |
B12 |
| pH 7.5 | 48 μl of HEPES pH 8.5 (1 M) 300 μl of Polyethylene glycol 400 (100 v/v) 100 μl of Magnesium chloride (2 M) 51 μl of HEPES pH 6.6 (1 M) 500 μl of water (100 v/v) |
C1 |
| pH 7.5 | 200 μl of 2-Propanol (100 v/v) 48 μl of HEPES pH 8.5 (1 M) 125 μl of tri-Sodium citrate (1.6 M) 51 μl of HEPES pH 6.6 (1 M) 575 μl of water (100 v/v) |
C2 |
| pH 7.5 | 533 μl of Potassium Sodium tartrate (1.5 M) 48 μl of HEPES pH 8.5 (1 M) 51 μl of HEPES pH 6.6 (1 M) 366 μl of water (100 v/v) |
C3 |
| pH 7.5 | 48 μl of HEPES pH 8.5 (1 M) 51 μl of HEPES pH 6.6 (1 M) 750 μl of Lithium sulfate (2 M) 150 μl of water (100 v/v) |
C4 |
| pH 7.5 | 400 μl of Potassium di-hydrogen phosphate (2 M) 48 μl of HEPES pH 8.5 (1 M) 160 μl of Sodium di-hydrogen phosphate (5 M) 51 μl of HEPES pH 6.6 (1 M) 340 μl of water (100 v/v) |
C5 |
| pH 7.5 | 48 μl of HEPES pH 8.5 (1 M) 875 μl of tri-Sodium citrate (1.6 M) 51 μl of HEPES pH 6.6 (1 M) 25 μl of water (100 v/v) |
C6 |
| pH 7.5 | 571 μl of Ammonium sulfate (3.5 M) 48 μl of HEPES pH 8.5 (1 M) 20 μl of Polyethylene glycol 400 (100 v/v) 51 μl of HEPES pH 6.6 (1 M) 308 μl of water (100 v/v) |
C7 |
| pH 7.5 | 100 μl of 2-Propanol (100 v/v) 48 μl of HEPES pH 8.5 (1 M) 400 μl of Polyethylene glycol 4,000 (50 w/v) 51 μl of HEPES pH 6.6 (1 M) 400 μl of water (100 v/v) |
C8 |
| pH 8.5 | 571 μl of Ammonium sulfate (3.5 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 328 μl of water (100 v/v) |
C9 |
| pH 8.5 | 100 μl of Magnesium chloride (2 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 600 μl of Polyethylene glycol 4,000 (50 w/v) 200 μl of water (100 v/v) |
C10 |
| pH 8.5 | 125 μl of tri-Sodium citrate (1.6 M) 300 μl of Polyethylene glycol 400 (100 v/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 475 μl of water (100 v/v) |
C11 |
| pH 8.5 | 100 μl of tris chloride BCC pH 8.5 (1 M) 600 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of Lithium sulfate (2 M) 200 μl of water (100 v/v) |
C12 |
| pH 8.5 | 300 μl of 2-Propanol (100 v/v) 200 μl of Ammonium acetate (1 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 400 μl of water (100 v/v) |
D1 |
| pH 8.5 | 66 μl of Sodium acetate (3 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 600 μl of Polyethylene glycol 4,000 (50 w/v) 233 μl of water (100 v/v) |
D2 |
| pH 8.5 | 160 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 740 μl of water (100 v/v) |
D3 |
| pH 8.5 | 800 μl of Ammonium di-hydrogen phosphate (2.5 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 100 μl of water (100 v/v) |
D4 |
| NA | 266 μl of Potassium Sodium tartrate (1.5 M) 733 μl of water (100 v/v) |
D5 |
| NA | 160 μl of Ammonium di-hydrogen phosphate (2.5 M) 840 μl of water (100 v/v) |
D6 |
| NA | 57 μl of Ammonium sulfate (3.5 M) 600 μl of Polyethylene glycol 8,000 (50 w/v) 342 μl of water (100 v/v) |
D7 |
| NA | 57 μl of Ammonium sulfate (3.5 M) 600 μl of Polyethylene glycol 4,000 (50 w/v) 342 μl of water (100 v/v) |
D8 |
| NA | 571 μl of Ammonium sulfate (3.5 M) 428 μl of water (100 v/v) |
D9 |
| NA | 571 μl of Sodium formate (7 M) 428 μl of water (100 v/v) |
D10 |
| NA | 25 μl of Potassium di-hydrogen phosphate (2 M) 400 μl of Polyethylene glycol 8,000 (50 w/v) 575 μl of water (100 v/v) |
D11 |
| NA | 600 μl of Polyethylene glycol 1,500 (50 w/v) 400 μl of water (100 v/v) |
D12 |
| NA | 200 μl of Magnesium formate (1 M) 800 μl of water (100 v/v) |
E1 |
| NA | 40 μl of Polyethylene glycol 8,000 (50 w/v) 500 μl of Lithium sulfate (2 M) 460 μl of water (100 v/v) |
E2 |
| NA | 300 μl of Polyethylene glycol 8,000 (50 w/v) 250 μl of Lithium sulfate (2 M) 450 μl of water (100 v/v) |
E3 | NA | ||
E4 | NA | ||
E5 | NA | ||
E6 | NA | ||
E7 | NA | ||
E8 | NA | ||
E9 | NA | ||
E10 | NA | ||
E11 | NA | ||
E12 | NA | ||
F1 | NA | ||
F2 | NA | ||
F3 | NA | ||
F4 | NA | ||
F5 | NA | ||
F6 | NA | ||
F7 | NA | ||
F8 | NA | ||
F9 | NA | ||
F10 | NA | ||
F11 | NA | ||
F12 | NA | ||
G1 | NA | ||
G2 | NA | ||
G3 | NA | ||
G4 | NA | ||
G5 | NA | ||
G6 | NA | ||
G7 | NA | ||
G8 | NA | ||
G9 | NA | ||
G10 | NA | ||
G11 | NA | ||
G12 | NA | ||
H1 | NA | ||
H2 | NA | ||
H3 | NA | ||
H4 | NA | ||
H5 | NA | ||
H6 | NA | ||
H7 | NA | ||
H8 | NA | ||
H9 | NA | ||
H10 | NA | ||
H11 | NA | ||
H12 | NA |