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Supplemental Information

Screen Description

Durham pH

Created 2018-03-08.

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Durham pH

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Durham pH

    Screen Structure

    Durham pH

    Well Count 96
    Number of distinct ingredients 30
    Internal diversity (score mean dist) 0.973

    Screen Composition

    More information about screen composition chart.
    2025-06-14T13:51:53.186016 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Durham pH
    Buffer 93
    Salt 30
    Water 2

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Durham pH Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Water 100 v/v -
    		NA
    1000 μl of Water (100 v/v)
    A2
    • Water 100 v/v -
    		NA
    1000 μl of Water (100 v/v)
    A3
    • Urea 8 M -
    		NA
    1000 μl of Urea (8 M)
    A4
    • tri-Sodium citrate 0.2 M pH 4.1
    		pH 4.1
    167 μl of tri-Sodium citrate pH 6.5 (1 M)
    32 μl of tri-Sodium citrate pH 4 (1 M)
    800 μl of water (100 v/v)
    A5
    • tri-Sodium citrate 0.2 M pH 4.6
    		pH 4.6
    175 μl of tri-Sodium citrate pH 6.5 (1 M)
    24 μl of tri-Sodium citrate pH 4 (1 M)
    800 μl of water (100 v/v)
    A6
    • tri-Sodium citrate 0.2 M pH 5.1
    		pH 5.1
    183 μl of tri-Sodium citrate pH 6.5 (1 M)
    17 μl of tri-Sodium citrate pH 4 (1 M)
    800 μl of water (100 v/v)
    A7
    • Sodium acetate 0.2 M pH 4.2
    		pH 4.2
    181 μl of Sodium acetate pH 4 (1 M)
    18 μl of Sodium acetate pH 5.5 (1 M)
    800 μl of water (100 v/v)
    A8
    • Sodium acetate 0.2 M pH 4.7
    		pH 4.7
    112 μl of Sodium acetate pH 4 (1 M)
    87 μl of Sodium acetate pH 5.5 (1 M)
    800 μl of water (100 v/v)
    A9
    • Sodium acetate 0.2 M pH 5.2
    		pH 5.2
    34 μl of Sodium acetate pH 4 (1 M)
    166 μl of Sodium acetate pH 5.5 (1 M)
    800 μl of water (100 v/v)
    A10
    • di-Sodium succinate 0.2 M pH 4.4
    		pH 4.4
    2 μl of di-Sodium succinate pH 6.5 (1 M)
    197 μl of di-Sodium succinate pH 4.3 (1 M)
    800 μl of water (100 v/v)
    A11
    • di-Sodium succinate 0.2 M pH 4.9
    		pH 4.9
    28 μl of di-Sodium succinate pH 6.5 (1 M)
    171 μl of di-Sodium succinate pH 4.3 (1 M)
    800 μl of water (100 v/v)
    A12
    • di-Sodium succinate 0.2 M pH 5.4
    		pH 5.4
    80 μl of di-Sodium succinate pH 6.5 (1 M)
    119 μl of di-Sodium succinate pH 4.3 (1 M)
    800 μl of water (100 v/v)
    B1
    • di-Sodium DL-malate 0.2 M pH 4.3
    		pH 4.3
    61 μl of di-Sodium DL-malate pH 7 (3 M)
    5 μl of di-Sodium DL-malate pH 2.9 (3 M)
    933 μl of water (100 v/v)
    B2
    • di-Sodium DL-malate 0.2 M pH 4.8
    		pH 4.8
    62 μl of di-Sodium DL-malate pH 7 (3 M)
    4 μl of di-Sodium DL-malate pH 2.9 (3 M)
    933 μl of water (100 v/v)
    B3
    • di-Sodium DL-malate 0.2 M pH 5.3
    		pH 5.3
    63 μl of di-Sodium DL-malate pH 7 (3 M)
    3 μl of di-Sodium DL-malate pH 2.9 (3 M)
    933 μl of water (100 v/v)
    B4
    • di-Sodium tartrate 0.2 M pH 4.3
    		pH 4.3
    140 μl of di-Sodium tartrate pH 4 (1 M)
    59 μl of di-Sodium tartrate pH 5.6 (1 M)
    800 μl of water (100 v/v)
    B5
    • di-Sodium tartrate 0.2 M pH 4.8
    		pH 4.8
    56 μl of di-Sodium tartrate pH 4 (1 M)
    143 μl of di-Sodium tartrate pH 5.6 (1 M)
    800 μl of water (100 v/v)
    B6
    • di-Sodium tartrate 0.2 M pH 5.3
    		pH 5.3
    12 μl of di-Sodium tartrate pH 4 (1 M)
    187 μl of di-Sodium tartrate pH 5.6 (1 M)
    800 μl of water (100 v/v)
    B7
    • Sodium Propionate 0.2 M pH 4.3
    		pH 4.3
    23 μl of Sodium Propionate pH 6 (1 M)
    177 μl of Sodium Propionate pH 4 (1 M)
    800 μl of water (100 v/v)
    B8
    • Sodium Propionate 0.2 M pH 4.8
    		pH 4.8
    84 μl of Sodium Propionate pH 6 (1 M)
    116 μl of Sodium Propionate pH 4 (1 M)
    800 μl of water (100 v/v)
    B9
    • Sodium Propionate 0.2 M pH 5.3
    		pH 5.3
    150 μl of Sodium Propionate pH 6 (1 M)
    49 μl of Sodium Propionate pH 4 (1 M)
    800 μl of water (100 v/v)
    B10
    • di-Sodium malonate 0.2 M pH 5.2
    		pH 5.2
    51 μl of di-Sodium malonate pH 5 (3.4 M)
    7 μl of di-Sodium malonate pH 7 (3.4 M)
    941 μl of water (100 v/v)
    B11
    • di-Sodium malonate 0.2 M pH 5.7
    		pH 5.7
    28 μl of di-Sodium malonate pH 5 (3.4 M)
    30 μl of di-Sodium malonate pH 7 (3.4 M)
    941 μl of water (100 v/v)
    B12
    • di-Sodium malonate 0.2 M pH 6.2
    		pH 6.2
    10 μl of di-Sodium malonate pH 5 (3.4 M)
    47 μl of di-Sodium malonate pH 7 (3.4 M)
    941 μl of water (100 v/v)
    C1
    • tri-Sodium citrate 0.2 M pH 5.5
    		pH 5.5
    188 μl of tri-Sodium citrate pH 6.5 (1 M)
    11 μl of tri-Sodium citrate pH 4 (1 M)
    800 μl of water (100 v/v)
    C2
    • tri-Sodium citrate 0.2 M pH 6
    		pH 6
    194 μl of tri-Sodium citrate pH 6.5 (1 M)
    5 μl of tri-Sodium citrate pH 4 (1 M)
    800 μl of water (100 v/v)
    C3
    • tri-Sodium citrate 0.2 M pH 6.5
    		pH 6.5
    198 μl of tri-Sodium citrate pH 6.5 (1 M)
    1 μl of tri-Sodium citrate pH 4 (1 M)
    800 μl of water (100 v/v)
    C4
    • di-Sodium succinate 0.2 M pH 5.6
    		pH 5.6
    107 μl of di-Sodium succinate pH 6.5 (1 M)
    92 μl of di-Sodium succinate pH 4.3 (1 M)
    800 μl of water (100 v/v)
    C5
    • di-Sodium succinate 0.2 M pH 6.1
    		pH 6.1
    169 μl of di-Sodium succinate pH 6.5 (1 M)
    30 μl of di-Sodium succinate pH 4.3 (1 M)
    800 μl of water (100 v/v)
    C6
    • di-Sodium succinate 0.2 M pH 6.6
    		pH 6.6
    188 μl of di-Sodium succinate pH 7 (1 M)
    11 μl of di-Sodium succinate pH 4.3 (1 M)
    800 μl of water (100 v/v)
    C7
    • MES 0.2 M pH 5.6
    		pH 5.6
    159 μl of MES pH 5 (1 M)
    40 μl of MES pH 7 (1 M)
    800 μl of water (100 v/v)
    C8
    • MES 0.2 M pH 6.1
    		pH 6.1
    95 μl of MES pH 5 (1 M)
    104 μl of MES pH 7 (1 M)
    800 μl of water (100 v/v)
    C9
    • MES 0.2 M pH 6.6
    		pH 6.6
    31 μl of MES pH 5 (1 M)
    168 μl of MES pH 7 (1 M)
    800 μl of water (100 v/v)
    C10
    • di-Sodium maleate 0.2 M pH 5.7
    		pH 5.7
    48 μl of di-Sodium maleate pH 7 (1 M)
    152 μl of di-Sodium maleate pH 4.5 (1 M)
    800 μl of water (100 v/v)
    C11
    • di-Sodium maleate 0.2 M pH 6.2
    		pH 6.2
    108 μl of di-Sodium maleate pH 7 (1 M)
    91 μl of di-Sodium maleate pH 4.5 (1 M)
    800 μl of water (100 v/v)
    C12
    • di-Sodium maleate 0.2 M pH 6.7
    		pH 6.7
    172 μl of di-Sodium maleate pH 7 (1 M)
    27 μl of di-Sodium maleate pH 4.5 (1 M)
    800 μl of water (100 v/v)
    D1
    • Sodium cacodylate 0.2 M pH 5.7
    		pH 5.7
    175 μl of Sodium cacodylate pH 5.5 (1 M)
    24 μl of Sodium cacodylate pH 6.7 (1 M)
    800 μl of water (100 v/v)
    D2
    • Sodium cacodylate 0.2 M pH 6.2
    		pH 6.2
    87 μl of Sodium cacodylate pH 5.5 (1 M)
    112 μl of Sodium cacodylate pH 6.7 (1 M)
    800 μl of water (100 v/v)
    D3
    • Sodium cacodylate 0.2 M pH 6.7
    		pH 6.7
    0 μl of Sodium cacodylate pH 5.5 (1 M)
    200 μl of Sodium cacodylate pH 6.7 (1 M)
    800 μl of water (100 v/v)
    D4
    • ADA 0.2 M pH 6.1
    		pH 6.1
    188 μl of ADA pH 6 (1 M)
    11 μl of ADA pH 7.5 (1 M)
    800 μl of water (100 v/v)
    D5
    • ADA 0.2 M pH 6.6
    		pH 6.6
    112 μl of ADA pH 6 (1 M)
    87 μl of ADA pH 7.5 (1 M)
    800 μl of water (100 v/v)
    D6
    • ADA 0.2 M pH 7.1
    		pH 7.1
    37 μl of ADA pH 6 (1 M)
    162 μl of ADA pH 7.5 (1 M)
    800 μl of water (100 v/v)
    D7
    • BIS-TRIS 0.2 M pH 6.1
    		pH 6.1
    155 μl of BIS-TRIS pH 5.5 (1 M)
    44 μl of BIS-TRIS pH 8 (1 M)
    800 μl of water (100 v/v)
    D8
    • BIS-TRIS 0.2 M pH 6.6
    		pH 6.6
    93 μl of BIS-TRIS pH 5.5 (1 M)
    106 μl of BIS-TRIS pH 8 (1 M)
    800 μl of water (100 v/v)
    D9
    • BIS-TRIS 0.2 M pH 7.1
    		pH 7.1
    38 μl of BIS-TRIS pH 5.5 (1 M)
    161 μl of BIS-TRIS pH 8 (1 M)
    800 μl of water (100 v/v)
    D10
    • N-(2-Acetamido)-2-aminoethanesulfonic acid 0.2 M pH 6.3
    		pH 6.3
    177 μl of N-(2-Acetamido)-2-aminoethanesulfonic acid pH 6.1 (1 M)
    22 μl of N-(2-Acetamido)-2-aminoethanesulfonic acid pH 7.5 (1 M)
    800 μl of water (100 v/v)
    D11
    • N-(2-Acetamido)-2-aminoethanesulfonic acid 0.2 M pH 6.8
    		pH 6.8
    100 μl of N-(2-Acetamido)-2-aminoethanesulfonic acid pH 6.1 (1 M)
    100 μl of N-(2-Acetamido)-2-aminoethanesulfonic acid pH 7.5 (1 M)
    800 μl of water (100 v/v)
    D12
    • N-(2-Acetamido)-2-aminoethanesulfonic acid 0.2 M pH 7.3
    		pH 7.3
    22 μl of N-(2-Acetamido)-2-aminoethanesulfonic acid pH 6.1 (1 M)
    177 μl of N-(2-Acetamido)-2-aminoethanesulfonic acid pH 7.5 (1 M)
    800 μl of water (100 v/v)
    E1
    • Sodium di-hydrogen phosphate 0.2 M pH 6.3
    		pH 6.3
    2 μl of Sodium di-hydrogen phosphate pH 7.5 (3 M)
    64 μl of Sodium di-hydrogen phosphate pH 6.2 (3 M)
    933 μl of water (100 v/v)
    E2
    • Sodium di-hydrogen phosphate 0.2 M pH 6.8
    		pH 6.8
    22 μl of Sodium di-hydrogen phosphate pH 7.5 (3 M)
    44 μl of Sodium di-hydrogen phosphate pH 6.2 (3 M)
    933 μl of water (100 v/v)
    E3
    • Sodium di-hydrogen phosphate 0.2 M pH 7.3
    		pH 7.3
    54 μl of Sodium di-hydrogen phosphate pH 7.5 (3 M)
    12 μl of Sodium di-hydrogen phosphate pH 6.2 (3 M)
    933 μl of water (100 v/v)
    E4
    • PIPES 0.2 M pH 6.3
    		pH 6.3
    29 μl of PIPES pH 7.5 (1 M)
    170 μl of PIPES pH 6 (1 M)
    800 μl of water (100 v/v)
    E5
    • PIPES 0.2 M pH 6.8
    		pH 6.8
    104 μl of PIPES pH 7.5 (1 M)
    95 μl of PIPES pH 6 (1 M)
    800 μl of water (100 v/v)
    E6
    • PIPES 0.2 M pH 7.3
    		pH 7.3
    178 μl of PIPES pH 7.5 (1 M)
    21 μl of PIPES pH 6 (1 M)
    800 μl of water (100 v/v)
    E7
    • Imidazole 0.2 M pH 6.6
    		pH 6.6
    161 μl of Imidazole pH 6.2 (1 M)
    38 μl of Imidazole pH 8 (1 M)
    800 μl of water (100 v/v)
    E8
    • Imidazole 0.2 M pH 7.1
    		pH 7.1
    91 μl of Imidazole pH 6.2 (1 M)
    108 μl of Imidazole pH 8 (1 M)
    800 μl of water (100 v/v)
    E9
    • Imidazole 0.2 M pH 7.6
    		pH 7.6
    28 μl of Imidazole pH 6.2 (1 M)
    171 μl of Imidazole pH 8 (1 M)
    800 μl of water (100 v/v)
    E10
    • MOPS 0.2 M pH 6.6
    		pH 6.6
    189 μl of MOPS pH 6.5 (1 M)
    10 μl of MOPS pH 7.9 (1 M)
    800 μl of water (100 v/v)
    E11
    • MOPS 0.2 M pH 7.1
    		pH 7.1
    117 μl of MOPS pH 6.5 (1 M)
    82 μl of MOPS pH 7.9 (1 M)
    800 μl of water (100 v/v)
    E12
    • MOPS 0.2 M pH 7.6
    		pH 7.6
    35 μl of MOPS pH 6.5 (1 M)
    164 μl of MOPS pH 7.9 (1 M)
    800 μl of water (100 v/v)
    F1
    • BIS-TRIS propane 0.2 M pH 6.6
    		pH 6.6
    15 μl of BIS-TRIS propane pH 9.7 (1 M)
    184 μl of BIS-TRIS propane pH 6.5 (1 M)
    800 μl of water (100 v/v)
    F2
    • BIS-TRIS propane 0.2 M pH 7.1
    		pH 7.1
    100 μl of BIS-TRIS propane pH 9.7 (1 M)
    100 μl of BIS-TRIS propane pH 6.5 (1 M)
    800 μl of water (100 v/v)
    F3
    • BIS-TRIS propane 0.2 M pH 7.6
    		pH 7.6
    159 μl of BIS-TRIS propane pH 9.7 (1 M)
    40 μl of BIS-TRIS propane pH 6.5 (1 M)
    800 μl of water (100 v/v)
    F4
    • HEPES 0.2 M pH 7
    		pH 7
    36 μl of HEPES pH 8.5 (1 M)
    163 μl of HEPES pH 6.5 (1 M)
    800 μl of water (100 v/v)
    F5
    • HEPES 0.2 M pH 7.5
    		pH 7.5
    100 μl of HEPES pH 8.5 (1 M)
    100 μl of HEPES pH 6.5 (1 M)
    800 μl of water (100 v/v)
    F6
    • HEPES 0.2 M pH 8
    		pH 8
    163 μl of HEPES pH 8.5 (1 M)
    36 μl of HEPES pH 6.5 (1 M)
    800 μl of water (100 v/v)
    F7
    • TRICINE 0.2 M pH 7.5
    		pH 7.5
    189 μl of TRICINE pH 7.4 (1 M)
    10 μl of TRICINE pH 8.8 (1 M)
    800 μl of water (100 v/v)
    F8
    • TRICINE 0.2 M pH 8
    		pH 8
    117 μl of TRICINE pH 7.4 (1 M)
    82 μl of TRICINE pH 8.8 (1 M)
    800 μl of water (100 v/v)
    F9
    • TRICINE 0.2 M pH 8.5
    		pH 8.5
    35 μl of TRICINE pH 7.4 (1 M)
    164 μl of TRICINE pH 8.8 (1 M)
    800 μl of water (100 v/v)
    F10
    • EPPS 0.2 M pH 7.5
    		pH 7.5
    22 μl of EPPS pH 8.7 (1 M)
    177 μl of EPPS pH 7.3 (1 M)
    800 μl of water (100 v/v)
    F11
    • EPPS 0.2 M pH 8
    		pH 8
    100 μl of EPPS pH 8.7 (1 M)
    100 μl of EPPS pH 7.3 (1 M)
    800 μl of water (100 v/v)
    F12
    • EPPS 0.2 M pH 8.5
    		pH 8.5
    177 μl of EPPS pH 8.7 (1 M)
    22 μl of EPPS pH 7.3 (1 M)
    800 μl of water (100 v/v)
    G1
    • tris chloride BCC 0.2 M pH 7.7
    		pH 7.7
    175 μl of tris chloride BCC pH 7.5 (1 M)
    24 μl of tris chloride BCC pH 9 (1 M)
    800 μl of water (100 v/v)
    G2
    • tris chloride BCC 0.2 M pH 8.2
    		pH 8.2
    96 μl of tris chloride BCC pH 7.5 (1 M)
    103 μl of tris chloride BCC pH 9 (1 M)
    800 μl of water (100 v/v)
    G3
    • tris chloride BCC 0.2 M pH 8.7
    		pH 8.7
    25 μl of tris chloride BCC pH 7.5 (1 M)
    174 μl of tris chloride BCC pH 9 (1 M)
    800 μl of water (100 v/v)
    G4
    • BICINE 0.2 M pH 7.7
    		pH 7.7
    22 μl of BICINE pH 9.3 (1 M)
    177 μl of BICINE pH 7.4 (1 M)
    800 μl of water (100 v/v)
    G5
    • BICINE 0.2 M pH 8.2
    		pH 8.2
    83 μl of BICINE pH 9.3 (1 M)
    117 μl of BICINE pH 7.4 (1 M)
    800 μl of water (100 v/v)
    G6
    • BICINE 0.2 M pH 8.7
    		pH 8.7
    151 μl of BICINE pH 9.3 (1 M)
    48 μl of BICINE pH 7.4 (1 M)
    800 μl of water (100 v/v)
    G7
    • TAPS 0.2 M pH 7.9
    		pH 7.9
    176 μl of TAPS pH 7.7 (1 M)
    23 μl of TAPS pH 9 (1 M)
    800 μl of water (100 v/v)
    G8
    • TAPS 0.2 M pH 8.4
    		pH 8.4
    94 μl of TAPS pH 7.7 (1 M)
    105 μl of TAPS pH 9 (1 M)
    800 μl of water (100 v/v)
    G9
    • TAPS 0.2 M pH 8.9
    		pH 8.9
    12 μl of TAPS pH 7.7 (1 M)
    187 μl of TAPS pH 9 (1 M)
    800 μl of water (100 v/v)
    G10
    • BIS-TRIS propane 0.2 M pH 8.5
    		pH 8.5
    194 μl of BIS-TRIS propane pH 9.7 (1 M)
    5 μl of BIS-TRIS propane pH 6.5 (1 M)
    800 μl of water (100 v/v)
    G11
    • BIS-TRIS propane 0.2 M pH 9
    		pH 9
    198 μl of BIS-TRIS propane pH 9.7 (1 M)
    1 μl of BIS-TRIS propane pH 6.5 (1 M)
    800 μl of water (100 v/v)
    G12
    • BIS-TRIS propane 0.2 M pH 9.5
    		pH 9.5
    199 μl of BIS-TRIS propane pH 9.7 (1 M)
    0 μl of BIS-TRIS propane pH 6.5 (1 M)
    800 μl of water (100 v/v)
    H1
    • di-Sodium tetraborate 0.2 M pH 8.6
    		pH 8.6
    190 μl of di-Sodium tetraborate pH 8.5 (1 M)
    10 μl of di-Sodium tetraborate pH 11 (1 M)
    800 μl of water (100 v/v)
    H2
    • di-Sodium tetraborate 0.2 M pH 9.1
    		pH 9.1
    123 μl of di-Sodium tetraborate pH 8.5 (1 M)
    76 μl of di-Sodium tetraborate pH 11 (1 M)
    800 μl of water (100 v/v)
    H3
    • di-Sodium tetraborate 0.2 M pH 9.6
    		pH 9.6
    57 μl of di-Sodium tetraborate pH 8.5 (1 M)
    142 μl of di-Sodium tetraborate pH 11 (1 M)
    800 μl of water (100 v/v)
    H4
    • CHES 0.2 M pH 8.8
    		pH 8.8
    37 μl of CHES pH 10 (1 M)
    162 μl of CHES pH 8 (1 M)
    800 μl of water (100 v/v)
    H5
    • CHES 0.2 M pH 9.3
    		pH 9.3
    97 μl of CHES pH 10 (1 M)
    102 μl of CHES pH 8 (1 M)
    800 μl of water (100 v/v)
    H6
    • CHES 0.2 M pH 9.8
    		pH 9.8
    174 μl of CHES pH 10 (1 M)
    25 μl of CHES pH 8 (1 M)
    800 μl of water (100 v/v)
    H7
    • Glycine 0.2 M pH 9.2
    		pH 9.2
    181 μl of Glycine pH 9 (1 M)
    18 μl of Glycine pH 10 (1 M)
    800 μl of water (100 v/v)
    H8
    • Glycine 0.2 M pH 9.7
    		pH 9.7
    112 μl of Glycine pH 9 (1 M)
    87 μl of Glycine pH 10 (1 M)
    800 μl of water (100 v/v)
    H9
    • Glycine 0.2 M pH 10
    		pH 10
    34 μl of Glycine pH 9 (1 M)
    166 μl of Glycine pH 10 (1 M)
    800 μl of water (100 v/v)
    H10
    • CAPS 0.2 M pH 9.9
    		pH 9.9
    26 μl of CAPS pH 12 (1 M)
    173 μl of CAPS pH 9.5 (1 M)
    800 μl of water (100 v/v)
    H11
    • CAPS 0.2 M pH 10
    		pH 10
    86 μl of CAPS pH 12 (1 M)
    114 μl of CAPS pH 9.5 (1 M)
    800 μl of water (100 v/v)
    H12
    • CAPS 0.2 M pH 11
    		pH 11
    152 μl of CAPS pH 12 (1 M)
    47 μl of CAPS pH 9.5 (1 M)
    800 μl of water (100 v/v)