Jump to page contents
Welcome to the new C6 website. This website aims preserve as many features as possible of the original C6 website.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Jump to well contents.

Supplemental Information

Screen Description

Durham pH

Created 2018-03-08.

Export

Durham pH

Barcodes

Durham pH

No barcodes found.

Screen Structure

Durham pH

Well Count 96
Number of distinct ingredients 30
Internal diversity (score mean dist) 0.973

Screen Composition

More information about screen composition chart.
2025-08-05T17:03:48.568998 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
ingredient type Durham pH
Buffer 93
Salt 30
Water 2

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Durham pH Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Water 100 v/v -
NA
1000.0 μl of Water (100 v/v)
A2
  • Water 100 v/v -
NA
1000.0 μl of Water (100 v/v)
A3
  • Urea 8 M -
NA
1000.0 μl of Urea (8 M)
A4
  • tri-Sodium citrate 0.2 M pH 4.1
pH 4.1
123.7 μl of tri-Sodium citrate pH 4 (1 M)
76.3 μl of tri-Sodium citrate pH 6.5 (1 M)
800.0 μl of water (100 v/v)
A5
  • tri-Sodium citrate 0.2 M pH 4.6
pH 4.6
109.9 μl of tri-Sodium citrate pH 4 (1 M)
90.1 μl of tri-Sodium citrate pH 6.5 (1 M)
800.0 μl of water (100 v/v)
A6
  • tri-Sodium citrate 0.2 M pH 5.1
pH 5.1
91.9 μl of tri-Sodium citrate pH 4 (1 M)
108.1 μl of tri-Sodium citrate pH 6.5 (1 M)
800.0 μl of water (100 v/v)
A7
  • Sodium acetate 0.2 M pH 4.2
pH 4.2
181.6 μl of Sodium acetate pH 4 (1 M)
18.4 μl of Sodium acetate pH 5.5 (1 M)
800.0 μl of water (100 v/v)
A8
  • Sodium acetate 0.2 M pH 4.7
pH 4.7
112.2 μl of Sodium acetate pH 4 (1 M)
87.8 μl of Sodium acetate pH 5.5 (1 M)
800.0 μl of water (100 v/v)
A9
  • Sodium acetate 0.2 M pH 5.2
pH 5.2
34.0 μl of Sodium acetate pH 4 (1 M)
166.0 μl of Sodium acetate pH 5.5 (1 M)
800.0 μl of water (100 v/v)
A10
  • di-Sodium succinate 0.2 M pH 4.4
pH 4.4
197.2 μl of di-Sodium succinate pH 4.3 (1 M)
2.8 μl of di-Sodium succinate pH 6.5 (1 M)
800.0 μl of water (100 v/v)
A11
  • di-Sodium succinate 0.2 M pH 4.9
pH 4.9
171.8 μl of di-Sodium succinate pH 4.3 (1 M)
28.2 μl of di-Sodium succinate pH 6.5 (1 M)
800.0 μl of water (100 v/v)
A12
  • di-Sodium succinate 0.2 M pH 5.4
pH 5.4
119.3 μl of di-Sodium succinate pH 4.3 (1 M)
80.7 μl of di-Sodium succinate pH 6.5 (1 M)
800.0 μl of water (100 v/v)
B1
  • di-Sodium DL-malate 0.2 M pH 4.3
pH 4.3
28.7 μl of di-Sodium DL-malate pH 2.9 (3 M)
38.0 μl of di-Sodium DL-malate pH 7 (3 M)
933.3 μl of water (100 v/v)
B2
  • di-Sodium DL-malate 0.2 M pH 4.8
pH 4.8
25.2 μl of di-Sodium DL-malate pH 2.9 (3 M)
41.5 μl of di-Sodium DL-malate pH 7 (3 M)
933.3 μl of water (100 v/v)
B3
  • di-Sodium DL-malate 0.2 M pH 5.3
pH 5.3
23.3 μl of di-Sodium DL-malate pH 2.9 (3 M)
43.3 μl of di-Sodium DL-malate pH 7 (3 M)
933.3 μl of water (100 v/v)
B4
  • di-Sodium tartrate 0.2 M pH 4.3
pH 4.3
140.7 μl of di-Sodium tartrate pH 4 (1 M)
59.3 μl of di-Sodium tartrate pH 5.6 (1 M)
800.0 μl of water (100 v/v)
B5
  • di-Sodium tartrate 0.2 M pH 4.8
pH 4.8
56.5 μl of di-Sodium tartrate pH 4 (1 M)
143.5 μl of di-Sodium tartrate pH 5.6 (1 M)
800.0 μl of water (100 v/v)
B6
  • di-Sodium tartrate 0.2 M pH 5.3
pH 5.3
12.3 μl of di-Sodium tartrate pH 4 (1 M)
187.7 μl of di-Sodium tartrate pH 5.6 (1 M)
800.0 μl of water (100 v/v)
B7
  • Sodium Propionate 0.2 M pH 4.3
pH 4.3
177.0 μl of Sodium Propionate pH 4 (1 M)
23.0 μl of Sodium Propionate pH 6 (1 M)
800.0 μl of water (100 v/v)
B8
  • Sodium Propionate 0.2 M pH 4.8
pH 4.8
116.0 μl of Sodium Propionate pH 4 (1 M)
84.0 μl of Sodium Propionate pH 6 (1 M)
800.0 μl of water (100 v/v)
B9
  • Sodium Propionate 0.2 M pH 5.3
pH 5.3
49.7 μl of Sodium Propionate pH 4 (1 M)
150.3 μl of Sodium Propionate pH 6 (1 M)
800.0 μl of water (100 v/v)
B10
  • di-Sodium malonate 0.2 M pH 5.2
pH 5.2
51.3 μl of di-Sodium malonate pH 5 (3.4 M)
7.6 μl of di-Sodium malonate pH 7 (3.4 M)
941.2 μl of water (100 v/v)
B11
  • di-Sodium malonate 0.2 M pH 5.7
pH 5.7
28.7 μl of di-Sodium malonate pH 5 (3.4 M)
30.1 μl of di-Sodium malonate pH 7 (3.4 M)
941.2 μl of water (100 v/v)
B12
  • di-Sodium malonate 0.2 M pH 6.2
pH 6.2
10.9 μl of di-Sodium malonate pH 5 (3.4 M)
47.9 μl of di-Sodium malonate pH 7 (3.4 M)
941.2 μl of water (100 v/v)
C1
  • tri-Sodium citrate 0.2 M pH 5.5
pH 5.5
72.6 μl of tri-Sodium citrate pH 4 (1 M)
127.4 μl of tri-Sodium citrate pH 6.5 (1 M)
800.0 μl of water (100 v/v)
C2
  • tri-Sodium citrate 0.2 M pH 6
pH 6
45.0 μl of tri-Sodium citrate pH 4 (1 M)
155.0 μl of tri-Sodium citrate pH 6.5 (1 M)
800.0 μl of water (100 v/v)
C3
  • tri-Sodium citrate 0.2 M pH 6.5
pH 6.5
14.8 μl of tri-Sodium citrate pH 4 (1 M)
185.2 μl of tri-Sodium citrate pH 6.5 (1 M)
800.0 μl of water (100 v/v)
C4
  • di-Sodium succinate 0.2 M pH 5.6
pH 5.6
92.4 μl of di-Sodium succinate pH 4.3 (1 M)
107.6 μl of di-Sodium succinate pH 6.5 (1 M)
800.0 μl of water (100 v/v)
C5
  • di-Sodium succinate 0.2 M pH 6.1
pH 6.1
30.6 μl of di-Sodium succinate pH 4.3 (1 M)
169.4 μl of di-Sodium succinate pH 6.5 (1 M)
800.0 μl of water (100 v/v)
C6
  • di-Sodium succinate 0.2 M pH 6.6
pH 6.6
11.5 μl of di-Sodium succinate pH 4.3 (1 M)
188.5 μl of di-Sodium succinate pH 7 (1 M)
800.0 μl of water (100 v/v)
C7
  • MES 0.2 M pH 5.6
pH 5.6
159.1 μl of MES pH 5 (1 M)
40.9 μl of MES pH 7 (1 M)
800.0 μl of water (100 v/v)
C8
  • MES 0.2 M pH 6.1
pH 6.1
95.3 μl of MES pH 5 (1 M)
104.7 μl of MES pH 7 (1 M)
800.0 μl of water (100 v/v)
C9
  • MES 0.2 M pH 6.6
pH 6.6
31.5 μl of MES pH 5 (1 M)
168.5 μl of MES pH 7 (1 M)
800.0 μl of water (100 v/v)
C10
  • di-Sodium maleate 0.2 M pH 5.7
pH 5.7
152.0 μl of di-Sodium maleate pH 4.5 (1 M)
48.0 μl of di-Sodium maleate pH 7 (1 M)
800.0 μl of water (100 v/v)
C11
  • di-Sodium maleate 0.2 M pH 6.2
pH 6.2
91.8 μl of di-Sodium maleate pH 4.5 (1 M)
108.2 μl of di-Sodium maleate pH 7 (1 M)
800.0 μl of water (100 v/v)
C12
  • di-Sodium maleate 0.2 M pH 6.7
pH 6.7
27.1 μl of di-Sodium maleate pH 4.5 (1 M)
172.9 μl of di-Sodium maleate pH 7 (1 M)
800.0 μl of water (100 v/v)
D1
  • Sodium cacodylate 0.2 M pH 5.7
pH 5.7
175.1 μl of Sodium cacodylate pH 5.5 (1 M)
24.9 μl of Sodium cacodylate pH 6.7 (1 M)
800.0 μl of water (100 v/v)
D2
  • Sodium cacodylate 0.2 M pH 6.2
pH 6.2
87.5 μl of Sodium cacodylate pH 5.5 (1 M)
112.5 μl of Sodium cacodylate pH 6.7 (1 M)
800.0 μl of water (100 v/v)
D3
  • Sodium cacodylate 0.2 M pH 6.7
pH 6.7
0.0 μl of Sodium cacodylate pH 5.5 (1 M)
200.0 μl of Sodium cacodylate pH 6.7 (1 M)
800.0 μl of water (100 v/v)
D4
  • ADA 0.2 M pH 6.1
pH 6.1
188.5 μl of ADA pH 6 (1 M)
11.5 μl of ADA pH 7.5 (1 M)
800.0 μl of water (100 v/v)
D5
  • ADA 0.2 M pH 6.6
pH 6.6
112.9 μl of ADA pH 6 (1 M)
87.1 μl of ADA pH 7.5 (1 M)
800.0 μl of water (100 v/v)
D6
  • ADA 0.2 M pH 7.1
pH 7.1
37.4 μl of ADA pH 6 (1 M)
162.6 μl of ADA pH 7.5 (1 M)
800.0 μl of water (100 v/v)
D7
  • BIS-TRIS 0.2 M pH 6.1
pH 6.1
155.9 μl of BIS-TRIS pH 5.5 (1 M)
44.1 μl of BIS-TRIS pH 8 (1 M)
800.0 μl of water (100 v/v)
D8
  • BIS-TRIS 0.2 M pH 6.6
pH 6.6
93.8 μl of BIS-TRIS pH 5.5 (1 M)
106.2 μl of BIS-TRIS pH 8 (1 M)
800.0 μl of water (100 v/v)
D9
  • BIS-TRIS 0.2 M pH 7.1
pH 7.1
38.7 μl of BIS-TRIS pH 5.5 (1 M)
161.3 μl of BIS-TRIS pH 8 (1 M)
800.0 μl of water (100 v/v)
D10
  • N-(2-Acetamido)-2-aminoethanesulfonic acid 0.2 M pH 6.3
pH 6.3
177.8 μl of N-(2-Acetamido)-2-aminoethanesulfonic acid pH 6.1 (1 M)
22.2 μl of N-(2-Acetamido)-2-aminoethanesulfonic acid pH 7.5 (1 M)
800.0 μl of water (100 v/v)
D11
  • N-(2-Acetamido)-2-aminoethanesulfonic acid 0.2 M pH 6.8
pH 6.8
100.0 μl of N-(2-Acetamido)-2-aminoethanesulfonic acid pH 6.1 (1 M)
100.0 μl of N-(2-Acetamido)-2-aminoethanesulfonic acid pH 7.5 (1 M)
800.0 μl of water (100 v/v)
D12
  • N-(2-Acetamido)-2-aminoethanesulfonic acid 0.2 M pH 7.3
pH 7.3
22.2 μl of N-(2-Acetamido)-2-aminoethanesulfonic acid pH 6.1 (1 M)
177.8 μl of N-(2-Acetamido)-2-aminoethanesulfonic acid pH 7.5 (1 M)
800.0 μl of water (100 v/v)
E1
  • Sodium di-hydrogen phosphate 0.2 M pH 6.3
pH 6.3
2.4 μl of Sodium di-hydrogen phosphate pH 7.5 (3 M)
64.2 μl of Sodium di-hydrogen phosphate pH 6.2 (3 M)
933.3 μl of water (100 v/v)
E2
  • Sodium di-hydrogen phosphate 0.2 M pH 6.8
pH 6.8
22.5 μl of Sodium di-hydrogen phosphate pH 7.5 (3 M)
44.2 μl of Sodium di-hydrogen phosphate pH 6.2 (3 M)
933.3 μl of water (100 v/v)
E3
  • Sodium di-hydrogen phosphate 0.2 M pH 7.3
pH 7.3
54.1 μl of Sodium di-hydrogen phosphate pH 7.5 (3 M)
12.6 μl of Sodium di-hydrogen phosphate pH 6.2 (3 M)
933.3 μl of water (100 v/v)
E4
  • PIPES 0.2 M pH 6.3
pH 6.3
170.3 μl of PIPES pH 6 (1 M)
29.7 μl of PIPES pH 7.5 (1 M)
800.0 μl of water (100 v/v)
E5
  • PIPES 0.2 M pH 6.8
pH 6.8
95.8 μl of PIPES pH 6 (1 M)
104.2 μl of PIPES pH 7.5 (1 M)
800.0 μl of water (100 v/v)
E6
  • PIPES 0.2 M pH 7.3
pH 7.3
21.2 μl of PIPES pH 6 (1 M)
178.8 μl of PIPES pH 7.5 (1 M)
800.0 μl of water (100 v/v)
E7
  • Imidazole 0.2 M pH 6.6
pH 6.6
161.7 μl of Imidazole pH 6.2 (1 M)
38.3 μl of Imidazole pH 8 (1 M)
800.0 μl of water (100 v/v)
E8
  • Imidazole 0.2 M pH 7.1
pH 7.1
91.1 μl of Imidazole pH 6.2 (1 M)
108.9 μl of Imidazole pH 8 (1 M)
800.0 μl of water (100 v/v)
E9
  • Imidazole 0.2 M pH 7.6
pH 7.6
28.4 μl of Imidazole pH 6.2 (1 M)
171.6 μl of Imidazole pH 8 (1 M)
800.0 μl of water (100 v/v)
E10
  • MOPS 0.2 M pH 6.6
pH 6.6
189.7 μl of MOPS pH 6.5 (1 M)
10.3 μl of MOPS pH 7.9 (1 M)
800.0 μl of water (100 v/v)
E11
  • MOPS 0.2 M pH 7.1
pH 7.1
117.2 μl of MOPS pH 6.5 (1 M)
82.8 μl of MOPS pH 7.9 (1 M)
800.0 μl of water (100 v/v)
E12
  • MOPS 0.2 M pH 7.6
pH 7.6
35.5 μl of MOPS pH 6.5 (1 M)
164.5 μl of MOPS pH 7.9 (1 M)
800.0 μl of water (100 v/v)
F1
  • BIS-TRIS propane 0.2 M pH 6.6
pH 6.6
184.1 μl of BIS-TRIS propane pH 6.5 (1 M)
15.9 μl of BIS-TRIS propane pH 9.7 (1 M)
800.0 μl of water (100 v/v)
F2
  • BIS-TRIS propane 0.2 M pH 7.1
pH 7.1
100.0 μl of BIS-TRIS propane pH 6.5 (1 M)
100.0 μl of BIS-TRIS propane pH 9.7 (1 M)
800.0 μl of water (100 v/v)
F3
  • BIS-TRIS propane 0.2 M pH 7.6
pH 7.6
40.8 μl of BIS-TRIS propane pH 6.5 (1 M)
159.2 μl of BIS-TRIS propane pH 9.7 (1 M)
800.0 μl of water (100 v/v)
F4
  • HEPES 0.2 M pH 7
pH 7
163.5 μl of HEPES pH 6.5 (1 M)
36.5 μl of HEPES pH 8.5 (1 M)
800.0 μl of water (100 v/v)
F5
  • HEPES 0.2 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 6.5 (1 M)
100.0 μl of HEPES pH 8.5 (1 M)
800.0 μl of water (100 v/v)
F6
  • HEPES 0.2 M pH 8
pH 8
36.5 μl of HEPES pH 6.5 (1 M)
163.5 μl of HEPES pH 8.5 (1 M)
800.0 μl of water (100 v/v)
F7
  • TRICINE 0.2 M pH 7.5
pH 7.5
189.7 μl of TRICINE pH 7.4 (1 M)
10.3 μl of TRICINE pH 8.8 (1 M)
800.0 μl of water (100 v/v)
F8
  • TRICINE 0.2 M pH 8
pH 8
117.2 μl of TRICINE pH 7.4 (1 M)
82.8 μl of TRICINE pH 8.8 (1 M)
800.0 μl of water (100 v/v)
F9
  • TRICINE 0.2 M pH 8.5
pH 8.5
35.5 μl of TRICINE pH 7.4 (1 M)
164.5 μl of TRICINE pH 8.8 (1 M)
800.0 μl of water (100 v/v)
F10
  • EPPS 0.2 M pH 7.5
pH 7.5
177.8 μl of EPPS pH 7.3 (1 M)
22.2 μl of EPPS pH 8.7 (1 M)
800.0 μl of water (100 v/v)
F11
  • EPPS 0.2 M pH 8
pH 8
100.0 μl of EPPS pH 7.3 (1 M)
100.0 μl of EPPS pH 8.7 (1 M)
800.0 μl of water (100 v/v)
F12
  • EPPS 0.2 M pH 8.5
pH 8.5
22.2 μl of EPPS pH 7.3 (1 M)
177.8 μl of EPPS pH 8.7 (1 M)
800.0 μl of water (100 v/v)
G1
  • tris chloride BCC 0.2 M pH 7.7
pH 7.7
175.6 μl of tris chloride BCC pH 7.5 (1 M)
24.4 μl of tris chloride BCC pH 9 (1 M)
800.0 μl of water (100 v/v)
G2
  • tris chloride BCC 0.2 M pH 8.2
pH 8.2
96.3 μl of tris chloride BCC pH 7.5 (1 M)
103.7 μl of tris chloride BCC pH 9 (1 M)
800.0 μl of water (100 v/v)
G3
  • tris chloride BCC 0.2 M pH 8.7
pH 8.7
25.9 μl of tris chloride BCC pH 7.5 (1 M)
174.1 μl of tris chloride BCC pH 9 (1 M)
800.0 μl of water (100 v/v)
G4
  • BICINE 0.2 M pH 7.7
pH 7.7
177.7 μl of BICINE pH 7.4 (1 M)
22.3 μl of BICINE pH 9.3 (1 M)
800.0 μl of water (100 v/v)
G5
  • BICINE 0.2 M pH 8.2
pH 8.2
117.0 μl of BICINE pH 7.4 (1 M)
83.0 μl of BICINE pH 9.3 (1 M)
800.0 μl of water (100 v/v)
G6
  • BICINE 0.2 M pH 8.7
pH 8.7
48.6 μl of BICINE pH 7.4 (1 M)
151.4 μl of BICINE pH 9.3 (1 M)
800.0 μl of water (100 v/v)
G7
  • TAPS 0.2 M pH 7.9
pH 7.9
176.6 μl of TAPS pH 7.7 (1 M)
23.4 μl of TAPS pH 9 (1 M)
800.0 μl of water (100 v/v)
G8
  • TAPS 0.2 M pH 8.4
pH 8.4
94.6 μl of TAPS pH 7.7 (1 M)
105.4 μl of TAPS pH 9 (1 M)
800.0 μl of water (100 v/v)
G9
  • TAPS 0.2 M pH 8.9
pH 8.9
12.5 μl of TAPS pH 7.7 (1 M)
187.5 μl of TAPS pH 9 (1 M)
800.0 μl of water (100 v/v)
G10
  • BIS-TRIS propane 0.2 M pH 8.5
pH 8.5
5.5 μl of BIS-TRIS propane pH 6.5 (1 M)
194.5 μl of BIS-TRIS propane pH 9.7 (1 M)
800.0 μl of water (100 v/v)
G11
  • BIS-TRIS propane 0.2 M pH 9
pH 9
1.5 μl of BIS-TRIS propane pH 6.5 (1 M)
198.5 μl of BIS-TRIS propane pH 9.7 (1 M)
800.0 μl of water (100 v/v)
G12
  • BIS-TRIS propane 0.2 M pH 9.5
pH 9.5
0.2 μl of BIS-TRIS propane pH 6.5 (1 M)
199.8 μl of BIS-TRIS propane pH 9.7 (1 M)
800.0 μl of water (100 v/v)
H1
  • di-Sodium tetraborate 0.2 M pH 8.6
pH 8.6
190.0 μl of di-Sodium tetraborate pH 8.5 (1 M)
10.0 μl of di-Sodium tetraborate pH 11 (1 M)
800.0 μl of water (100 v/v)
H2
  • di-Sodium tetraborate 0.2 M pH 9.1
pH 9.1
123.9 μl of di-Sodium tetraborate pH 8.5 (1 M)
76.1 μl of di-Sodium tetraborate pH 11 (1 M)
800.0 μl of water (100 v/v)
H3
  • di-Sodium tetraborate 0.2 M pH 9.6
pH 9.6
57.9 μl of di-Sodium tetraborate pH 8.5 (1 M)
142.1 μl of di-Sodium tetraborate pH 11 (1 M)
800.0 μl of water (100 v/v)
H4
  • CHES 0.2 M pH 8.8
pH 8.8
162.8 μl of CHES pH 8 (1 M)
37.2 μl of CHES pH 10 (1 M)
800.0 μl of water (100 v/v)
H5
  • CHES 0.2 M pH 9.3
pH 9.3
102.3 μl of CHES pH 8 (1 M)
97.7 μl of CHES pH 10 (1 M)
800.0 μl of water (100 v/v)
H6
  • CHES 0.2 M pH 9.8
pH 9.8
25.7 μl of CHES pH 8 (1 M)
174.3 μl of CHES pH 10 (1 M)
800.0 μl of water (100 v/v)
H7
  • Glycine 0.2 M pH 9.2
pH 9.2
181.6 μl of Glycine pH 9 (1 M)
18.4 μl of Glycine pH 10 (1 M)
800.0 μl of water (100 v/v)
H8
  • Glycine 0.2 M pH 9.7
pH 9.7
112.2 μl of Glycine pH 9 (1 M)
87.8 μl of Glycine pH 10 (1 M)
800.0 μl of water (100 v/v)
H9
  • Glycine 0.2 M pH 10
pH 10
34.0 μl of Glycine pH 9 (1 M)
166.0 μl of Glycine pH 10 (1 M)
800.0 μl of water (100 v/v)
H10
  • CAPS 0.2 M pH 9.9
pH 9.9
173.1 μl of CAPS pH 9.5 (1 M)
26.9 μl of CAPS pH 12 (1 M)
800.0 μl of water (100 v/v)
H11
  • CAPS 0.2 M pH 10
pH 10
114.0 μl of CAPS pH 9.5 (1 M)
86.0 μl of CAPS pH 12 (1 M)
800.0 μl of water (100 v/v)
H12
  • CAPS 0.2 M pH 11
pH 11
47.4 μl of CAPS pH 9.5 (1 M)
152.6 μl of CAPS pH 12 (1 M)
800.0 μl of water (100 v/v)