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Supplemental Information

Screen Description

Durham pH

Created 2018-03-08.

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Durham pH

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Durham pH

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Screen Structure

Durham pH

Well Count 96
Number of distinct ingredients 30
Internal diversity (score mean dist) 0.973

Screen Composition

More information about screen composition chart.
2025-08-16T09:05:39.470713 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Durham pH
Buffer 93
Salt 30
Water 2

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Durham pH Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Water 100 v/v -
NA
1000.0 μl of Water (100 v/v)
A2
  • Water 100 v/v -
NA
1000.0 μl of Water (100 v/v)
A3
  • Urea 8 M -
NA
1000.0 μl of Urea (8 M)
A4
  • tri-Sodium citrate 0.2 M pH 4.1
pH 4.1
123.7 μl of tri-Sodium citrate pH 4 (1 M)
76.3 μl of tri-Sodium citrate pH 6.5 (1 M)
800.0 μl of water (100 v/v)
A5
  • tri-Sodium citrate 0.2 M pH 4.6
pH 4.6
109.9 μl of tri-Sodium citrate pH 4 (1 M)
90.1 μl of tri-Sodium citrate pH 6.5 (1 M)
800.0 μl of water (100 v/v)
A6
  • tri-Sodium citrate 0.2 M pH 5.1
pH 5.1
91.9 μl of tri-Sodium citrate pH 4 (1 M)
108.1 μl of tri-Sodium citrate pH 6.5 (1 M)
800.0 μl of water (100 v/v)
A7
  • Sodium acetate 0.2 M pH 4.2
pH 4.2
181.6 μl of Sodium acetate pH 4 (1 M)
18.4 μl of Sodium acetate pH 5.5 (1 M)
800.0 μl of water (100 v/v)
A8
  • Sodium acetate 0.2 M pH 4.7
pH 4.7
112.2 μl of Sodium acetate pH 4 (1 M)
87.8 μl of Sodium acetate pH 5.5 (1 M)
800.0 μl of water (100 v/v)
A9
  • Sodium acetate 0.2 M pH 5.2
pH 5.2
34.0 μl of Sodium acetate pH 4 (1 M)
166.0 μl of Sodium acetate pH 5.5 (1 M)
800.0 μl of water (100 v/v)
A10
  • di-Sodium succinate 0.2 M pH 4.4
pH 4.4
197.2 μl of di-Sodium succinate pH 4.3 (1 M)
2.8 μl of di-Sodium succinate pH 6.5 (1 M)
800.0 μl of water (100 v/v)
A11
  • di-Sodium succinate 0.2 M pH 4.9
pH 4.9
171.8 μl of di-Sodium succinate pH 4.3 (1 M)
28.2 μl of di-Sodium succinate pH 6.5 (1 M)
800.0 μl of water (100 v/v)
A12
  • di-Sodium succinate 0.2 M pH 5.4
pH 5.4
119.3 μl of di-Sodium succinate pH 4.3 (1 M)
80.7 μl of di-Sodium succinate pH 6.5 (1 M)
800.0 μl of water (100 v/v)
B1
  • di-Sodium DL-malate 0.2 M pH 4.3
pH 4.3
28.7 μl of di-Sodium DL-malate pH 2.9 (3 M)
38.0 μl of di-Sodium DL-malate pH 7 (3 M)
933.3 μl of water (100 v/v)
B2
  • di-Sodium DL-malate 0.2 M pH 4.8
pH 4.8
25.2 μl of di-Sodium DL-malate pH 2.9 (3 M)
41.5 μl of di-Sodium DL-malate pH 7 (3 M)
933.3 μl of water (100 v/v)
B3
  • di-Sodium DL-malate 0.2 M pH 5.3
pH 5.3
23.3 μl of di-Sodium DL-malate pH 2.9 (3 M)
43.3 μl of di-Sodium DL-malate pH 7 (3 M)
933.3 μl of water (100 v/v)
B4
  • di-Sodium tartrate 0.2 M pH 4.3
pH 4.3
140.7 μl of di-Sodium tartrate pH 4 (1 M)
59.3 μl of di-Sodium tartrate pH 5.6 (1 M)
800.0 μl of water (100 v/v)
B5
  • di-Sodium tartrate 0.2 M pH 4.8
pH 4.8
56.5 μl of di-Sodium tartrate pH 4 (1 M)
143.5 μl of di-Sodium tartrate pH 5.6 (1 M)
800.0 μl of water (100 v/v)
B6
  • di-Sodium tartrate 0.2 M pH 5.3
pH 5.3
12.3 μl of di-Sodium tartrate pH 4 (1 M)
187.7 μl of di-Sodium tartrate pH 5.6 (1 M)
800.0 μl of water (100 v/v)
B7
  • Sodium Propionate 0.2 M pH 4.3
pH 4.3
177.0 μl of Sodium Propionate pH 4 (1 M)
23.0 μl of Sodium Propionate pH 6 (1 M)
800.0 μl of water (100 v/v)
B8
  • Sodium Propionate 0.2 M pH 4.8
pH 4.8
116.0 μl of Sodium Propionate pH 4 (1 M)
84.0 μl of Sodium Propionate pH 6 (1 M)
800.0 μl of water (100 v/v)
B9
  • Sodium Propionate 0.2 M pH 5.3
pH 5.3
49.7 μl of Sodium Propionate pH 4 (1 M)
150.3 μl of Sodium Propionate pH 6 (1 M)
800.0 μl of water (100 v/v)
B10
  • di-Sodium malonate 0.2 M pH 5.2
pH 5.2
51.3 μl of di-Sodium malonate pH 5 (3.4 M)
7.6 μl of di-Sodium malonate pH 7 (3.4 M)
941.2 μl of water (100 v/v)
B11
  • di-Sodium malonate 0.2 M pH 5.7
pH 5.7
28.7 μl of di-Sodium malonate pH 5 (3.4 M)
30.1 μl of di-Sodium malonate pH 7 (3.4 M)
941.2 μl of water (100 v/v)
B12
  • di-Sodium malonate 0.2 M pH 6.2
pH 6.2
10.9 μl of di-Sodium malonate pH 5 (3.4 M)
47.9 μl of di-Sodium malonate pH 7 (3.4 M)
941.2 μl of water (100 v/v)
C1
  • tri-Sodium citrate 0.2 M pH 5.5
pH 5.5
72.6 μl of tri-Sodium citrate pH 4 (1 M)
127.4 μl of tri-Sodium citrate pH 6.5 (1 M)
800.0 μl of water (100 v/v)
C2
  • tri-Sodium citrate 0.2 M pH 6
pH 6
45.0 μl of tri-Sodium citrate pH 4 (1 M)
155.0 μl of tri-Sodium citrate pH 6.5 (1 M)
800.0 μl of water (100 v/v)
C3
  • tri-Sodium citrate 0.2 M pH 6.5
pH 6.5
14.8 μl of tri-Sodium citrate pH 4 (1 M)
185.2 μl of tri-Sodium citrate pH 6.5 (1 M)
800.0 μl of water (100 v/v)
C4
  • di-Sodium succinate 0.2 M pH 5.6
pH 5.6
92.4 μl of di-Sodium succinate pH 4.3 (1 M)
107.6 μl of di-Sodium succinate pH 6.5 (1 M)
800.0 μl of water (100 v/v)
C5
  • di-Sodium succinate 0.2 M pH 6.1
pH 6.1
30.6 μl of di-Sodium succinate pH 4.3 (1 M)
169.4 μl of di-Sodium succinate pH 6.5 (1 M)
800.0 μl of water (100 v/v)
C6
  • di-Sodium succinate 0.2 M pH 6.6
pH 6.6
11.5 μl of di-Sodium succinate pH 4.3 (1 M)
188.5 μl of di-Sodium succinate pH 7 (1 M)
800.0 μl of water (100 v/v)
C7
  • MES 0.2 M pH 5.6
pH 5.6
159.1 μl of MES pH 5 (1 M)
40.9 μl of MES pH 7 (1 M)
800.0 μl of water (100 v/v)
C8
  • MES 0.2 M pH 6.1
pH 6.1
95.3 μl of MES pH 5 (1 M)
104.7 μl of MES pH 7 (1 M)
800.0 μl of water (100 v/v)
C9
  • MES 0.2 M pH 6.6
pH 6.6
31.5 μl of MES pH 5 (1 M)
168.5 μl of MES pH 7 (1 M)
800.0 μl of water (100 v/v)
C10
  • di-Sodium maleate 0.2 M pH 5.7
pH 5.7
152.0 μl of di-Sodium maleate pH 4.5 (1 M)
48.0 μl of di-Sodium maleate pH 7 (1 M)
800.0 μl of water (100 v/v)
C11
  • di-Sodium maleate 0.2 M pH 6.2
pH 6.2
91.8 μl of di-Sodium maleate pH 4.5 (1 M)
108.2 μl of di-Sodium maleate pH 7 (1 M)
800.0 μl of water (100 v/v)
C12
  • di-Sodium maleate 0.2 M pH 6.7
pH 6.7
27.1 μl of di-Sodium maleate pH 4.5 (1 M)
172.9 μl of di-Sodium maleate pH 7 (1 M)
800.0 μl of water (100 v/v)
D1
  • Sodium cacodylate 0.2 M pH 5.7
pH 5.7
175.1 μl of Sodium cacodylate pH 5.5 (1 M)
24.9 μl of Sodium cacodylate pH 6.7 (1 M)
800.0 μl of water (100 v/v)
D2
  • Sodium cacodylate 0.2 M pH 6.2
pH 6.2
87.5 μl of Sodium cacodylate pH 5.5 (1 M)
112.5 μl of Sodium cacodylate pH 6.7 (1 M)
800.0 μl of water (100 v/v)
D3
  • Sodium cacodylate 0.2 M pH 6.7
pH 6.7
0.0 μl of Sodium cacodylate pH 5.5 (1 M)
200.0 μl of Sodium cacodylate pH 6.7 (1 M)
800.0 μl of water (100 v/v)
D4
  • ADA 0.2 M pH 6.1
pH 6.1
188.5 μl of ADA pH 6 (1 M)
11.5 μl of ADA pH 7.5 (1 M)
800.0 μl of water (100 v/v)
D5
  • ADA 0.2 M pH 6.6
pH 6.6
112.9 μl of ADA pH 6 (1 M)
87.1 μl of ADA pH 7.5 (1 M)
800.0 μl of water (100 v/v)
D6
  • ADA 0.2 M pH 7.1
pH 7.1
37.4 μl of ADA pH 6 (1 M)
162.6 μl of ADA pH 7.5 (1 M)
800.0 μl of water (100 v/v)
D7
  • BIS-TRIS 0.2 M pH 6.1
pH 6.1
155.9 μl of BIS-TRIS pH 5.5 (1 M)
44.1 μl of BIS-TRIS pH 8 (1 M)
800.0 μl of water (100 v/v)
D8
  • BIS-TRIS 0.2 M pH 6.6
pH 6.6
93.8 μl of BIS-TRIS pH 5.5 (1 M)
106.2 μl of BIS-TRIS pH 8 (1 M)
800.0 μl of water (100 v/v)
D9
  • BIS-TRIS 0.2 M pH 7.1
pH 7.1
38.7 μl of BIS-TRIS pH 5.5 (1 M)
161.3 μl of BIS-TRIS pH 8 (1 M)
800.0 μl of water (100 v/v)
D10
  • N-(2-Acetamido)-2-aminoethanesulfonic acid 0.2 M pH 6.3
pH 6.3
177.8 μl of N-(2-Acetamido)-2-aminoethanesulfonic acid pH 6.1 (1 M)
22.2 μl of N-(2-Acetamido)-2-aminoethanesulfonic acid pH 7.5 (1 M)
800.0 μl of water (100 v/v)
D11
  • N-(2-Acetamido)-2-aminoethanesulfonic acid 0.2 M pH 6.8
pH 6.8
100.0 μl of N-(2-Acetamido)-2-aminoethanesulfonic acid pH 6.1 (1 M)
100.0 μl of N-(2-Acetamido)-2-aminoethanesulfonic acid pH 7.5 (1 M)
800.0 μl of water (100 v/v)
D12
  • N-(2-Acetamido)-2-aminoethanesulfonic acid 0.2 M pH 7.3
pH 7.3
22.2 μl of N-(2-Acetamido)-2-aminoethanesulfonic acid pH 6.1 (1 M)
177.8 μl of N-(2-Acetamido)-2-aminoethanesulfonic acid pH 7.5 (1 M)
800.0 μl of water (100 v/v)
E1
  • Sodium di-hydrogen phosphate 0.2 M pH 6.3
pH 6.3
2.4 μl of Sodium di-hydrogen phosphate pH 7.5 (3 M)
64.2 μl of Sodium di-hydrogen phosphate pH 6.2 (3 M)
933.3 μl of water (100 v/v)
E2
  • Sodium di-hydrogen phosphate 0.2 M pH 6.8
pH 6.8
22.5 μl of Sodium di-hydrogen phosphate pH 7.5 (3 M)
44.2 μl of Sodium di-hydrogen phosphate pH 6.2 (3 M)
933.3 μl of water (100 v/v)
E3
  • Sodium di-hydrogen phosphate 0.2 M pH 7.3
pH 7.3
54.1 μl of Sodium di-hydrogen phosphate pH 7.5 (3 M)
12.6 μl of Sodium di-hydrogen phosphate pH 6.2 (3 M)
933.3 μl of water (100 v/v)
E4
  • PIPES 0.2 M pH 6.3
pH 6.3
170.3 μl of PIPES pH 6 (1 M)
29.7 μl of PIPES pH 7.5 (1 M)
800.0 μl of water (100 v/v)
E5
  • PIPES 0.2 M pH 6.8
pH 6.8
95.8 μl of PIPES pH 6 (1 M)
104.2 μl of PIPES pH 7.5 (1 M)
800.0 μl of water (100 v/v)
E6
  • PIPES 0.2 M pH 7.3
pH 7.3
21.2 μl of PIPES pH 6 (1 M)
178.8 μl of PIPES pH 7.5 (1 M)
800.0 μl of water (100 v/v)
E7
  • Imidazole 0.2 M pH 6.6
pH 6.6
161.7 μl of Imidazole pH 6.2 (1 M)
38.3 μl of Imidazole pH 8 (1 M)
800.0 μl of water (100 v/v)
E8
  • Imidazole 0.2 M pH 7.1
pH 7.1
91.1 μl of Imidazole pH 6.2 (1 M)
108.9 μl of Imidazole pH 8 (1 M)
800.0 μl of water (100 v/v)
E9
  • Imidazole 0.2 M pH 7.6
pH 7.6
28.4 μl of Imidazole pH 6.2 (1 M)
171.6 μl of Imidazole pH 8 (1 M)
800.0 μl of water (100 v/v)
E10
  • MOPS 0.2 M pH 6.6
pH 6.6
189.7 μl of MOPS pH 6.5 (1 M)
10.3 μl of MOPS pH 7.9 (1 M)
800.0 μl of water (100 v/v)
E11
  • MOPS 0.2 M pH 7.1
pH 7.1
117.2 μl of MOPS pH 6.5 (1 M)
82.8 μl of MOPS pH 7.9 (1 M)
800.0 μl of water (100 v/v)
E12
  • MOPS 0.2 M pH 7.6
pH 7.6
35.5 μl of MOPS pH 6.5 (1 M)
164.5 μl of MOPS pH 7.9 (1 M)
800.0 μl of water (100 v/v)
F1
  • BIS-TRIS propane 0.2 M pH 6.6
pH 6.6
184.1 μl of BIS-TRIS propane pH 6.5 (1 M)
15.9 μl of BIS-TRIS propane pH 9.7 (1 M)
800.0 μl of water (100 v/v)
F2
  • BIS-TRIS propane 0.2 M pH 7.1
pH 7.1
100.0 μl of BIS-TRIS propane pH 6.5 (1 M)
100.0 μl of BIS-TRIS propane pH 9.7 (1 M)
800.0 μl of water (100 v/v)
F3
  • BIS-TRIS propane 0.2 M pH 7.6
pH 7.6
40.8 μl of BIS-TRIS propane pH 6.5 (1 M)
159.2 μl of BIS-TRIS propane pH 9.7 (1 M)
800.0 μl of water (100 v/v)
F4
  • HEPES 0.2 M pH 7
pH 7
163.5 μl of HEPES pH 6.5 (1 M)
36.5 μl of HEPES pH 8.5 (1 M)
800.0 μl of water (100 v/v)
F5
  • HEPES 0.2 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 6.5 (1 M)
100.0 μl of HEPES pH 8.5 (1 M)
800.0 μl of water (100 v/v)
F6
  • HEPES 0.2 M pH 8
pH 8
36.5 μl of HEPES pH 6.5 (1 M)
163.5 μl of HEPES pH 8.5 (1 M)
800.0 μl of water (100 v/v)
F7
  • TRICINE 0.2 M pH 7.5
pH 7.5
189.7 μl of TRICINE pH 7.4 (1 M)
10.3 μl of TRICINE pH 8.8 (1 M)
800.0 μl of water (100 v/v)
F8
  • TRICINE 0.2 M pH 8
pH 8
117.2 μl of TRICINE pH 7.4 (1 M)
82.8 μl of TRICINE pH 8.8 (1 M)
800.0 μl of water (100 v/v)
F9
  • TRICINE 0.2 M pH 8.5
pH 8.5
35.5 μl of TRICINE pH 7.4 (1 M)
164.5 μl of TRICINE pH 8.8 (1 M)
800.0 μl of water (100 v/v)
F10
  • EPPS 0.2 M pH 7.5
pH 7.5
177.8 μl of EPPS pH 7.3 (1 M)
22.2 μl of EPPS pH 8.7 (1 M)
800.0 μl of water (100 v/v)
F11
  • EPPS 0.2 M pH 8
pH 8
100.0 μl of EPPS pH 7.3 (1 M)
100.0 μl of EPPS pH 8.7 (1 M)
800.0 μl of water (100 v/v)
F12
  • EPPS 0.2 M pH 8.5
pH 8.5
22.2 μl of EPPS pH 7.3 (1 M)
177.8 μl of EPPS pH 8.7 (1 M)
800.0 μl of water (100 v/v)
G1
  • tris chloride BCC 0.2 M pH 7.7
pH 7.7
175.6 μl of tris chloride BCC pH 7.5 (1 M)
24.4 μl of tris chloride BCC pH 9 (1 M)
800.0 μl of water (100 v/v)
G2
  • tris chloride BCC 0.2 M pH 8.2
pH 8.2
96.3 μl of tris chloride BCC pH 7.5 (1 M)
103.7 μl of tris chloride BCC pH 9 (1 M)
800.0 μl of water (100 v/v)
G3
  • tris chloride BCC 0.2 M pH 8.7
pH 8.7
25.9 μl of tris chloride BCC pH 7.5 (1 M)
174.1 μl of tris chloride BCC pH 9 (1 M)
800.0 μl of water (100 v/v)
G4
  • BICINE 0.2 M pH 7.7
pH 7.7
177.7 μl of BICINE pH 7.4 (1 M)
22.3 μl of BICINE pH 9.3 (1 M)
800.0 μl of water (100 v/v)
G5
  • BICINE 0.2 M pH 8.2
pH 8.2
117.0 μl of BICINE pH 7.4 (1 M)
83.0 μl of BICINE pH 9.3 (1 M)
800.0 μl of water (100 v/v)
G6
  • BICINE 0.2 M pH 8.7
pH 8.7
48.6 μl of BICINE pH 7.4 (1 M)
151.4 μl of BICINE pH 9.3 (1 M)
800.0 μl of water (100 v/v)
G7
  • TAPS 0.2 M pH 7.9
pH 7.9
176.6 μl of TAPS pH 7.7 (1 M)
23.4 μl of TAPS pH 9 (1 M)
800.0 μl of water (100 v/v)
G8
  • TAPS 0.2 M pH 8.4
pH 8.4
94.6 μl of TAPS pH 7.7 (1 M)
105.4 μl of TAPS pH 9 (1 M)
800.0 μl of water (100 v/v)
G9
  • TAPS 0.2 M pH 8.9
pH 8.9
12.5 μl of TAPS pH 7.7 (1 M)
187.5 μl of TAPS pH 9 (1 M)
800.0 μl of water (100 v/v)
G10
  • BIS-TRIS propane 0.2 M pH 8.5
pH 8.5
5.5 μl of BIS-TRIS propane pH 6.5 (1 M)
194.5 μl of BIS-TRIS propane pH 9.7 (1 M)
800.0 μl of water (100 v/v)
G11
  • BIS-TRIS propane 0.2 M pH 9
pH 9
1.5 μl of BIS-TRIS propane pH 6.5 (1 M)
198.5 μl of BIS-TRIS propane pH 9.7 (1 M)
800.0 μl of water (100 v/v)
G12
  • BIS-TRIS propane 0.2 M pH 9.5
pH 9.5
0.2 μl of BIS-TRIS propane pH 6.5 (1 M)
199.8 μl of BIS-TRIS propane pH 9.7 (1 M)
800.0 μl of water (100 v/v)
H1
  • di-Sodium tetraborate 0.2 M pH 8.6
pH 8.6
190.0 μl of di-Sodium tetraborate pH 8.5 (1 M)
10.0 μl of di-Sodium tetraborate pH 11 (1 M)
800.0 μl of water (100 v/v)
H2
  • di-Sodium tetraborate 0.2 M pH 9.1
pH 9.1
123.9 μl of di-Sodium tetraborate pH 8.5 (1 M)
76.1 μl of di-Sodium tetraborate pH 11 (1 M)
800.0 μl of water (100 v/v)
H3
  • di-Sodium tetraborate 0.2 M pH 9.6
pH 9.6
57.9 μl of di-Sodium tetraborate pH 8.5 (1 M)
142.1 μl of di-Sodium tetraborate pH 11 (1 M)
800.0 μl of water (100 v/v)
H4
  • CHES 0.2 M pH 8.8
pH 8.8
162.8 μl of CHES pH 8 (1 M)
37.2 μl of CHES pH 10 (1 M)
800.0 μl of water (100 v/v)
H5
  • CHES 0.2 M pH 9.3
pH 9.3
102.3 μl of CHES pH 8 (1 M)
97.7 μl of CHES pH 10 (1 M)
800.0 μl of water (100 v/v)
H6
  • CHES 0.2 M pH 9.8
pH 9.8
25.7 μl of CHES pH 8 (1 M)
174.3 μl of CHES pH 10 (1 M)
800.0 μl of water (100 v/v)
H7
  • Glycine 0.2 M pH 9.2
pH 9.2
181.6 μl of Glycine pH 9 (1 M)
18.4 μl of Glycine pH 10 (1 M)
800.0 μl of water (100 v/v)
H8
  • Glycine 0.2 M pH 9.7
pH 9.7
112.2 μl of Glycine pH 9 (1 M)
87.8 μl of Glycine pH 10 (1 M)
800.0 μl of water (100 v/v)
H9
  • Glycine 0.2 M pH 10
pH 10
34.0 μl of Glycine pH 9 (1 M)
166.0 μl of Glycine pH 10 (1 M)
800.0 μl of water (100 v/v)
H10
  • CAPS 0.2 M pH 9.9
pH 9.9
173.1 μl of CAPS pH 9.5 (1 M)
26.9 μl of CAPS pH 12 (1 M)
800.0 μl of water (100 v/v)
H11
  • CAPS 0.2 M pH 10
pH 10
114.0 μl of CAPS pH 9.5 (1 M)
86.0 μl of CAPS pH 12 (1 M)
800.0 μl of water (100 v/v)
H12
  • CAPS 0.2 M pH 11
pH 11
47.4 μl of CAPS pH 9.5 (1 M)
152.6 μl of CAPS pH 12 (1 M)
800.0 μl of water (100 v/v)