C6 - Screen Contents

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Supplemental Information

Screen Description

Classic 03

Classics / Classic 03

Created 2009-11-16.

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Classic 03

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Classic 03

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Screen Structure

Classic 03

Well Count	24
Number of distinct ingredients	15
Internal diversity (score mean dist)	0.691

Screen Composition

More information about screen composition chart.

ingredient type	Classic 03
Buffer	20
Cryoprotectant	1
Organic (non-volatile)	1
Organic (volatile)	9
Polymer	24
Salt	28

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Classic 03 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	tris chloride BCC 0.1 M pH 8.5 Lithium chloride 0.8 M - Polyethylene glycol 4,000 8 w/v - 🔍	pH 8.5	160.0 μl of Lithium chloride (5 M) 160.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 580.0 μl of water (100 v/v)
A2	2-Propanol 20 w/v - Polyethylene glycol 4,000 10 w/v - 🔍	NA	400.0 μl of 2-Propanol (50 w/v) 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 400.0 μl of water (100 v/v)
A3	tri-Sodium citrate 0.1 M pH 5.6 2-Propanol 10 w/v - Polyethylene glycol 4,000 10 w/v - 🔍	pH 5.6	200.0 μl of 2-Propanol (50 w/v) 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 500.0 μl of water (100 v/v)
A4	HEPES 0.1 M pH 7.5 2-Propanol 5 w/v - Polyethylene glycol 4,000 10 w/v - 🔍	pH 7.5	100.0 μl of 2-Propanol (50 w/v) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 600.0 μl of water (100 v/v)
A5	HEPES 0.1 M pH 7.5 2-Propanol 20 w/v - Polyethylene glycol 4,000 10 w/v - 🔍	pH 7.5	400.0 μl of 2-Propanol (50 w/v) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 300.0 μl of water (100 v/v)
A6	Ammonium sulfate 0.2 M - Sodium acetate 0.1 M pH 4.6 Polyethylene glycol 4,000 12 w/v - 🔍	pH 4.6	66.7 μl of Ammonium sulfate (3 M) 240.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 593.3 μl of water (100 v/v)
B1	Ammonium sulfate 0.2 M - Polyethylene glycol 4,000 15 w/v - 🔍	NA	66.7 μl of Ammonium sulfate (3 M) 300.0 μl of Polyethylene glycol 4,000 (50 w/v) 633.3 μl of water (100 v/v)
B2	Ammonium sulfate 0.2 M - tri-Sodium citrate 0.1 M pH 5.6 Polyethylene glycol 4,000 15 w/v - 🔍	pH 5.6	66.7 μl of Ammonium sulfate (3 M) 300.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 533.3 μl of water (100 v/v)
B3	HEPES 0.1 M pH 7.5 Ammonium sulfate 0.2 M - 2-Propanol 10 w/v - Polyethylene glycol 4,000 16 w/v - 🔍	pH 7.5	200.0 μl of 2-Propanol (50 w/v) 66.7 μl of Ammonium sulfate (3 M) 100.0 μl of HEPES pH 7.5 (1 M) 320.0 μl of Polyethylene glycol 4,000 (50 w/v) 313.3 μl of water (100 v/v)
B4	Ammonium sulfate 0.2 M - Polyethylene glycol 4,000 20 w/v - 🔍	NA	66.7 μl of Ammonium sulfate (3 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 533.3 μl of water (100 v/v)
B5	Magnesium sulfate 0.2 M - Glycerol 10 w/v - Polyethylene glycol 4,000 20 w/v - 🔍	NA	200.0 μl of Glycerol (50 w/v) 200.0 μl of Magnesium sulfate (1 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 200.0 μl of water (100 v/v)
B6	tri-Sodium citrate 0.1 M - 2-Propanol 5 w/v - Polyethylene glycol 4,000 20 w/v - 🔍	NA	100.0 μl of 2-Propanol (50 w/v) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 50.0 μl of tri-Sodium citrate (2 M) 450.0 μl of water (100 v/v)
C1	tri-Sodium citrate 0.1 M - 2-Propanol 20 w/v - Polyethylene glycol 4,000 20 w/v - 🔍	NA	400.0 μl of 2-Propanol (50 w/v) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 50.0 μl of tri-Sodium citrate (2 M) 150.0 μl of water (100 v/v)
C2	Sodium chloride 0.6 M - MES 0.1 M pH 6.5 Polyethylene glycol 4,000 20 w/v - 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 120.0 μl of Sodium chloride (5 M) 380.0 μl of water (100 v/v)
C3	HEPES 0.1 M pH 7.5 2-Propanol 10 w/v - Polyethylene glycol 4,000 20 w/v - 🔍	pH 7.5	200.0 μl of 2-Propanol (50 w/v) 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 300.0 μl of water (100 v/v)
C4	Sodium acetate 0.1 M - Ammonium sulfate 0.2 M - Polyethylene glycol 4,000 22 w/v - 🔍	NA	66.7 μl of Ammonium sulfate (3 M) 440.0 μl of Polyethylene glycol 4,000 (50 w/v) 50.0 μl of Sodium acetate (2 M) 443.3 μl of water (100 v/v)
C5	Ammonium sulfate 0.2 M - Sodium acetate 0.1 M pH 4.6 Polyethylene glycol 4,000 25 w/v - 🔍	pH 4.6	66.7 μl of Ammonium sulfate (3 M) 500.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 333.3 μl of water (100 v/v)
C6	Ammonium sulfate 0.2 M - tri-Sodium citrate 0.1 M pH 5.6 Polyethylene glycol 4,000 25 w/v - 🔍	pH 5.6	66.7 μl of Ammonium sulfate (3 M) 500.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 333.3 μl of water (100 v/v)
D1	HEPES 0.1 M pH 7.5 Sodium acetate 0.1 M - Lithium sulfate 0.2 M - Polyethylene glycol 4,000 25 w/v - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 66.7 μl of Lithium sulfate (3 M) 500.0 μl of Polyethylene glycol 4,000 (50 w/v) 50.0 μl of Sodium acetate (2 M) 283.3 μl of water (100 v/v)
D2	Sodium acetate 0.1 M - 2-Propanol 8 w/v - Polyethylene glycol 4,000 25 w/v - 🔍	NA	160.0 μl of 2-Propanol (50 w/v) 500.0 μl of Polyethylene glycol 4,000 (50 w/v) 50.0 μl of Sodium acetate (2 M) 290.0 μl of water (100 v/v)
D3	Ammonium sulfate 0.2 M - Polyethylene glycol 4,000 30 w/v - 🔍	NA	66.7 μl of Ammonium sulfate (3 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 333.3 μl of water (100 v/v)
D4	Ammonium sulfate 0.2 M - Sodium acetate 0.1 M pH 4.6 Polyethylene glycol 4,000 30 w/v - 🔍	pH 4.6	66.7 μl of Ammonium sulfate (3 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 233.3 μl of water (100 v/v)
D5	Ammonium sulfate 0.1 M - tri-Sodium citrate 0.1 M pH 5.6 Polyethylene glycol 4,000 30 w/v - 🔍	pH 5.6	33.3 μl of Ammonium sulfate (3 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 266.7 μl of water (100 v/v)
D6	Lithium chloride 0.8 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 4,000 32 w/v - 🔍	pH 8.5	160.0 μl of Lithium chloride (5 M) 640.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 100.0 μl of water (100 v/v)