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Supplemental Information

Screen Description

Classic 03

Classics / Classic 03
Created 2009-11-16.

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Classic 03

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Classic 03

    Screen Structure

    Classic 03

    Well Count 24
    Number of distinct ingredients 15
    Internal diversity (score mean dist) 0.691

    Screen Composition

    More information about screen composition chart.
    2025-06-14T13:39:53.277348 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Classic 03
    Buffer 20
    Cryoprotectant 1
    Organic (non-volatile) 1
    Organic (volatile) 9
    Polymer 24
    Salt 28

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Classic 03 Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • tris chloride BCC 0.1 M pH 8.5
    • Lithium chloride 0.8 M -
    • Polyethylene glycol 4,000 8 w/v -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    160 μl of Polyethylene glycol 4,000 (50 w/v)
    160 μl of Lithium chloride (5 M)
    580 μl of water (100 v/v)
    A2
    • 2-Propanol 20 w/v -
    • Polyethylene glycol 4,000 10 w/v -
    		NA
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    400 μl of 2-Propanol (50 w/v)
    400 μl of water (100 v/v)
    A3
    • tri-Sodium citrate 0.1 M pH 5.6
    • 2-Propanol 10 w/v -
    • Polyethylene glycol 4,000 10 w/v -
    		pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    200 μl of 2-Propanol (50 w/v)
    500 μl of water (100 v/v)
    A4
    • HEPES 0.1 M pH 7.5
    • 2-Propanol 5 w/v -
    • Polyethylene glycol 4,000 10 w/v -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of 2-Propanol (50 w/v)
    600 μl of water (100 v/v)
    A5
    • HEPES 0.1 M pH 7.5
    • 2-Propanol 20 w/v -
    • Polyethylene glycol 4,000 10 w/v -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    400 μl of 2-Propanol (50 w/v)
    300 μl of water (100 v/v)
    A6
    • Ammonium sulfate 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 12 w/v -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    66 μl of Ammonium sulfate (3 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    593 μl of water (100 v/v)
    B1
    • Ammonium sulfate 0.2 M -
    • Polyethylene glycol 4,000 15 w/v -
    		NA
    66 μl of Ammonium sulfate (3 M)
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    633 μl of water (100 v/v)
    B2
    • Ammonium sulfate 0.2 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 4,000 15 w/v -
    		pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    66 μl of Ammonium sulfate (3 M)
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    533 μl of water (100 v/v)
    B3
    • HEPES 0.1 M pH 7.5
    • Ammonium sulfate 0.2 M -
    • 2-Propanol 10 w/v -
    • Polyethylene glycol 4,000 16 w/v -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    66 μl of Ammonium sulfate (3 M)
    320 μl of Polyethylene glycol 4,000 (50 w/v)
    200 μl of 2-Propanol (50 w/v)
    313 μl of water (100 v/v)
    B4
    • Ammonium sulfate 0.2 M -
    • Polyethylene glycol 4,000 20 w/v -
    		NA
    66 μl of Ammonium sulfate (3 M)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    533 μl of water (100 v/v)
    B5
    • Magnesium sulfate 0.2 M -
    • Glycerol 10 w/v -
    • Polyethylene glycol 4,000 20 w/v -
    		NA
    200 μl of Glycerol (50 w/v)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    200 μl of Magnesium sulfate (1 M)
    200 μl of water (100 v/v)
    B6
    • tri-Sodium citrate 0.1 M -
    • 2-Propanol 5 w/v -
    • Polyethylene glycol 4,000 20 w/v -
    		NA
    50 μl of tri-Sodium citrate (2 M)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of 2-Propanol (50 w/v)
    450 μl of water (100 v/v)
    C1
    • tri-Sodium citrate 0.1 M -
    • 2-Propanol 20 w/v -
    • Polyethylene glycol 4,000 20 w/v -
    		NA
    50 μl of tri-Sodium citrate (2 M)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    400 μl of 2-Propanol (50 w/v)
    150 μl of water (100 v/v)
    C2
    • Sodium chloride 0.6 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 4,000 20 w/v -
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    120 μl of Sodium chloride (5 M)
    380 μl of water (100 v/v)
    C3
    • HEPES 0.1 M pH 7.5
    • 2-Propanol 10 w/v -
    • Polyethylene glycol 4,000 20 w/v -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    200 μl of 2-Propanol (50 w/v)
    300 μl of water (100 v/v)
    C4
    • Sodium acetate 0.1 M -
    • Ammonium sulfate 0.2 M -
    • Polyethylene glycol 4,000 22 w/v -
    		NA
    50 μl of Sodium acetate (2 M)
    66 μl of Ammonium sulfate (3 M)
    440 μl of Polyethylene glycol 4,000 (50 w/v)
    443 μl of water (100 v/v)
    C5
    • Ammonium sulfate 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 25 w/v -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    66 μl of Ammonium sulfate (3 M)
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    333 μl of water (100 v/v)
    C6
    • Ammonium sulfate 0.2 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 4,000 25 w/v -
    		pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    66 μl of Ammonium sulfate (3 M)
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    333 μl of water (100 v/v)
    D1
    • HEPES 0.1 M pH 7.5
    • Sodium acetate 0.1 M -
    • Lithium sulfate 0.2 M -
    • Polyethylene glycol 4,000 25 w/v -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    66 μl of Lithium sulfate (3 M)
    50 μl of Sodium acetate (2 M)
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    283 μl of water (100 v/v)
    D2
    • Sodium acetate 0.1 M -
    • 2-Propanol 8 w/v -
    • Polyethylene glycol 4,000 25 w/v -
    		NA
    50 μl of Sodium acetate (2 M)
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    160 μl of 2-Propanol (50 w/v)
    290 μl of water (100 v/v)
    D3
    • Ammonium sulfate 0.2 M -
    • Polyethylene glycol 4,000 30 w/v -
    		NA
    66 μl of Ammonium sulfate (3 M)
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    333 μl of water (100 v/v)
    D4
    • Ammonium sulfate 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 30 w/v -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    66 μl of Ammonium sulfate (3 M)
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    233 μl of water (100 v/v)
    D5
    • Ammonium sulfate 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 4,000 30 w/v -
    		pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    33 μl of Ammonium sulfate (3 M)
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    266 μl of water (100 v/v)
    D6
    • Lithium chloride 0.8 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 32 w/v -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    640 μl of Polyethylene glycol 4,000 (50 w/v)
    160 μl of Lithium chloride (5 M)
    100 μl of water (100 v/v)