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Supplemental Information

Screen Description

Solubility_HTS

Optimization / Solubility_HTS
Created 2013-03-25.

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Solubility_HTS

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Solubility_HTS

    Screen Structure

    Solubility_HTS

    Well Count 96
    Number of distinct ingredients 22
    Internal diversity (score mean dist) 0.847

    Screen Composition

    More information about screen composition chart.
    2025-06-14T13:35:37.631663 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Solubility_HTS
    Buffer 94
    Cryoprotectant 47
    Organic (non-volatile) 47
    Salt 58

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Solubility_HTS Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Acetonitrile 20 v/v -
    		NA
    1000 μl of Acetonitrile (20 v/v)
    A2
    • MES 0.1 M pH 5.5
    		pH 5.5
    100 μl of MES pH 5.5 (1 M)
    900 μl of water (100 v/v)
    A3
    • ADA 0.1 M pH 6
    		pH 6
    100 μl of ADA pH 6 (1 M)
    900 μl of water (100 v/v)
    A4
    • MES 0.1 M pH 6.5
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    900 μl of water (100 v/v)
    A5
    • BIS-TRIS 0.1 M pH 6.5
    		pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    900 μl of water (100 v/v)
    A6
    • di-Potassium hydrogen phosphate 0.1 M pH 6.5
    		pH 6.5
    50 μl of di-Potassium hydrogen phosphate pH 6.5 (2 M)
    950 μl of water (100 v/v)
    A7
    • PIPES 0.1 M pH 7
    		pH 7
    100 μl of PIPES pH 7 (1 M)
    900 μl of water (100 v/v)
    A8
    • Imidazole 0.1 M pH 7
    		pH 7
    100 μl of Imidazole pH 7 (1 M)
    900 μl of water (100 v/v)
    A9
    • ADA 0.1 M pH 7
    		pH 7
    100 μl of ADA pH 7 (1 M)
    900 μl of water (100 v/v)
    A10
    • MOPS 0.1 M pH 7
    		pH 7
    100 μl of MOPS pH 7 (1 M)
    900 μl of water (100 v/v)
    A11
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    900 μl of water (100 v/v)
    A12
    • di-Potassium hydrogen phosphate 0.1 M pH 7.5
    		pH 7.5
    100 μl of di-Potassium hydrogen phosphate pH 7.5 (1 M)
    900 μl of water (100 v/v)
    B1
    • DIPSO 0.1 M pH 7.5
    		pH 7.5
    100 μl of DIPSO pH 7.5 (1 M)
    900 μl of water (100 v/v)
    B2
    • TRICINE 0.1 M pH 7.5
    		pH 7.5
    100 μl of TRICINE pH 7.5 (1 M)
    900 μl of water (100 v/v)
    B3
    • HEPES 0.1 M pH 8
    		pH 8
    100 μl of HEPES pH 8 (1 M)
    900 μl of water (100 v/v)
    B4
    • BICINE 0.1 M pH 8
    		pH 8
    100 μl of BICINE pH 8 (1 M)
    900 μl of water (100 v/v)
    B5
    • tris chloride BCC 0.1 M pH 8
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    900 μl of water (100 v/v)
    B6
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    900 μl of water (100 v/v)
    B7
    • TABS 0.1 M pH 8.5
    		pH 8.5
    100 μl of TABS pH 8.5 (1 M)
    900 μl of water (100 v/v)
    B8
    • TAPS 0.1 M pH 8.5
    		pH 8.5
    100 μl of TAPS pH 8.5 (1 M)
    900 μl of water (100 v/v)
    B9
    • CAPSO 0.1 M pH 9
    		pH 9
    100 μl of CAPSO pH 9 (1 M)
    900 μl of water (100 v/v)
    B10
    • CHES 0.1 M pH 9
    		pH 9
    100 μl of CHES pH 9 (1 M)
    900 μl of water (100 v/v)
    B11
    • Glycine 0.1 M pH 9.5
    		pH 9.5
    50 μl of Glycine pH 9.5 (2 M)
    950 μl of water (100 v/v)
    B12
    • CAPS 0.1 M pH 10
    		pH 10
    100 μl of CAPS pH 10 (1 M)
    900 μl of water (100 v/v)
    C1
    • Potassium acetate 0.1 M pH 5
    • Sodium chloride 0.15 M -
    		pH 5
    30 μl of Sodium chloride (5 M)
    100 μl of Potassium acetate pH 5 (1 M)
    870 μl of water (100 v/v)
    C2
    • MES 0.1 M pH 5.5
    • Sodium chloride 0.15 M -
    		pH 5.5
    30 μl of Sodium chloride (5 M)
    100 μl of MES pH 5.5 (1 M)
    870 μl of water (100 v/v)
    C3
    • ADA 0.1 M pH 6
    • Sodium chloride 0.15 M -
    		pH 6
    30 μl of Sodium chloride (5 M)
    100 μl of ADA pH 6 (1 M)
    870 μl of water (100 v/v)
    C4
    • MES 0.1 M pH 6.5
    • Sodium chloride 0.15 M -
    		pH 6.5
    30 μl of Sodium chloride (5 M)
    100 μl of MES pH 6.5 (1 M)
    870 μl of water (100 v/v)
    C5
    • BIS-TRIS 0.1 M pH 6.5
    • Sodium chloride 0.15 M -
    		pH 6.5
    30 μl of Sodium chloride (5 M)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    870 μl of water (100 v/v)
    C6
    • di-Potassium hydrogen phosphate 0.1 M pH 6.5
    • Sodium chloride 0.15 M -
    		pH 6.5
    50 μl of di-Potassium hydrogen phosphate pH 6.5 (2 M)
    30 μl of Sodium chloride (5 M)
    920 μl of water (100 v/v)
    C7
    • PIPES 0.1 M pH 7
    • Sodium chloride 0.15 M -
    		pH 7
    30 μl of Sodium chloride (5 M)
    100 μl of PIPES pH 7 (1 M)
    870 μl of water (100 v/v)
    C8
    • Imidazole 0.1 M pH 7
    • Sodium chloride 0.15 M -
    		pH 7
    30 μl of Sodium chloride (5 M)
    100 μl of Imidazole pH 7 (1 M)
    870 μl of water (100 v/v)
    C9
    • ADA 0.1 M pH 7
    • Sodium chloride 0.15 M -
    		pH 7
    30 μl of Sodium chloride (5 M)
    100 μl of ADA pH 7 (1 M)
    870 μl of water (100 v/v)
    C10
    • MOPS 0.1 M pH 7
    • Sodium chloride 0.15 M -
    		pH 7
    30 μl of Sodium chloride (5 M)
    100 μl of MOPS pH 7 (1 M)
    870 μl of water (100 v/v)
    C11
    • HEPES 0.1 M pH 7.5
    • Sodium chloride 0.15 M -
    		pH 7.5
    30 μl of Sodium chloride (5 M)
    100 μl of HEPES pH 7.5 (1 M)
    870 μl of water (100 v/v)
    C12
    • di-Potassium hydrogen phosphate 0.1 M pH 7.5
    • Sodium chloride 0.15 M -
    		pH 7.5
    100 μl of di-Potassium hydrogen phosphate pH 7.5 (1 M)
    30 μl of Sodium chloride (5 M)
    870 μl of water (100 v/v)
    D1
    • DIPSO 0.1 M pH 7.5
    • Sodium chloride 0.15 M -
    		pH 7.5
    100 μl of DIPSO pH 7.5 (1 M)
    30 μl of Sodium chloride (5 M)
    870 μl of water (100 v/v)
    D2
    • TRICINE 0.1 M pH 7.5
    • Sodium chloride 0.15 M -
    		pH 7.5
    100 μl of TRICINE pH 7.5 (1 M)
    30 μl of Sodium chloride (5 M)
    870 μl of water (100 v/v)
    D3
    • HEPES 0.1 M pH 8
    • Sodium chloride 0.15 M -
    		pH 8
    100 μl of HEPES pH 8 (1 M)
    30 μl of Sodium chloride (5 M)
    870 μl of water (100 v/v)
    D4
    • BICINE 0.1 M pH 8
    • Sodium chloride 0.15 M -
    		pH 8
    30 μl of Sodium chloride (5 M)
    100 μl of BICINE pH 8 (1 M)
    870 μl of water (100 v/v)
    D5
    • tris chloride BCC 0.1 M pH 8
    • Sodium chloride 0.15 M -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    30 μl of Sodium chloride (5 M)
    870 μl of water (100 v/v)
    D6
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium chloride 0.15 M -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    30 μl of Sodium chloride (5 M)
    870 μl of water (100 v/v)
    D7
    • TABS 0.1 M pH 8.5
    • Sodium chloride 0.15 M -
    		pH 8.5
    30 μl of Sodium chloride (5 M)
    100 μl of TABS pH 8.5 (1 M)
    870 μl of water (100 v/v)
    D8
    • TAPS 0.1 M pH 8.5
    • Sodium chloride 0.15 M -
    		pH 8.5
    30 μl of Sodium chloride (5 M)
    100 μl of TAPS pH 8.5 (1 M)
    870 μl of water (100 v/v)
    D9
    • CAPSO 0.1 M pH 9
    • Sodium chloride 0.15 M -
    		pH 9
    100 μl of CAPSO pH 9 (1 M)
    30 μl of Sodium chloride (5 M)
    870 μl of water (100 v/v)
    D10
    • CHES 0.1 M pH 9
    • Sodium chloride 0.15 M -
    		pH 9
    30 μl of Sodium chloride (5 M)
    100 μl of CHES pH 9 (1 M)
    870 μl of water (100 v/v)
    D11
    • Glycine 0.1 M pH 9.5
    • Sodium chloride 0.15 M -
    		pH 9.5
    30 μl of Sodium chloride (5 M)
    50 μl of Glycine pH 9.5 (2 M)
    920 μl of water (100 v/v)
    D12
    • CAPS 0.1 M pH 10
    • Sodium chloride 0.15 M -
    		pH 10
    30 μl of Sodium chloride (5 M)
    100 μl of CAPS pH 10 (1 M)
    870 μl of water (100 v/v)
    E1
    • Potassium acetate 0.1 M pH 5
    • Glycerol 5 v/v -
    		pH 5
    100 μl of Glycerol (50 v/v)
    100 μl of Potassium acetate pH 5 (1 M)
    800 μl of water (100 v/v)
    E2
    • MES 0.1 M pH 5.5
    • Glycerol 5 v/v -
    		pH 5.5
    100 μl of Glycerol (50 v/v)
    100 μl of MES pH 5.5 (1 M)
    800 μl of water (100 v/v)
    E3
    • ADA 0.1 M pH 6
    • Glycerol 5 v/v -
    		pH 6
    100 μl of Glycerol (50 v/v)
    100 μl of ADA pH 6 (1 M)
    800 μl of water (100 v/v)
    E4
    • MES 0.1 M pH 6.5
    • Glycerol 5 v/v -
    		pH 6.5
    100 μl of Glycerol (50 v/v)
    100 μl of MES pH 6.5 (1 M)
    800 μl of water (100 v/v)
    E5
    • BIS-TRIS 0.1 M pH 6.5
    • Glycerol 5 v/v -
    		pH 6.5
    100 μl of Glycerol (50 v/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    800 μl of water (100 v/v)
    E6
    • di-Potassium hydrogen phosphate 0.1 M pH 6.5
    • Glycerol 5 v/v -
    		pH 6.5
    50 μl of di-Potassium hydrogen phosphate pH 6.5 (2 M)
    100 μl of Glycerol (50 v/v)
    850 μl of water (100 v/v)
    E7
    • PIPES 0.1 M pH 7
    • Glycerol 5 v/v -
    		pH 7
    100 μl of PIPES pH 7 (1 M)
    100 μl of Glycerol (50 v/v)
    800 μl of water (100 v/v)
    E8
    • Imidazole 0.1 M pH 7
    • Glycerol 5 v/v -
    		pH 7
    100 μl of Glycerol (50 v/v)
    100 μl of Imidazole pH 7 (1 M)
    800 μl of water (100 v/v)
    E9
    • ADA 0.1 M pH 7
    • Glycerol 5 v/v -
    		pH 7
    100 μl of Glycerol (50 v/v)
    100 μl of ADA pH 7 (1 M)
    800 μl of water (100 v/v)
    E10
    • MOPS 0.1 M pH 7
    • Glycerol 5 v/v -
    		pH 7
    100 μl of Glycerol (50 v/v)
    100 μl of MOPS pH 7 (1 M)
    800 μl of water (100 v/v)
    E11
    • HEPES 0.1 M pH 7.5
    • Glycerol 5 v/v -
    		pH 7.5
    100 μl of Glycerol (50 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    800 μl of water (100 v/v)
    E12
    • di-Potassium hydrogen phosphate 0.1 M pH 7.5
    • Glycerol 5 v/v -
    		pH 7.5
    100 μl of di-Potassium hydrogen phosphate pH 7.5 (1 M)
    100 μl of Glycerol (50 v/v)
    800 μl of water (100 v/v)
    F1
    • DIPSO 0.1 M pH 7.5
    • Glycerol 5 v/v -
    		pH 7.5
    100 μl of DIPSO pH 7.5 (1 M)
    100 μl of Glycerol (50 v/v)
    800 μl of water (100 v/v)
    F2
    • TRICINE 0.1 M pH 7.5
    • Glycerol 5 v/v -
    		pH 7.5
    100 μl of TRICINE pH 7.5 (1 M)
    100 μl of Glycerol (50 v/v)
    800 μl of water (100 v/v)
    F3
    • HEPES 0.1 M pH 8
    • Glycerol 5 v/v -
    		pH 8
    100 μl of HEPES pH 8 (1 M)
    100 μl of Glycerol (50 v/v)
    800 μl of water (100 v/v)
    F4
    • BICINE 0.1 M pH 8
    • Glycerol 5 v/v -
    		pH 8
    100 μl of Glycerol (50 v/v)
    100 μl of BICINE pH 8 (1 M)
    800 μl of water (100 v/v)
    F5
    • tris chloride BCC 0.1 M pH 8
    • Glycerol 5 v/v -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    100 μl of Glycerol (50 v/v)
    800 μl of water (100 v/v)
    F6
    • tris chloride BCC 0.1 M pH 8.5
    • Glycerol 5 v/v -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    100 μl of Glycerol (50 v/v)
    800 μl of water (100 v/v)
    F7
    • TABS 0.1 M pH 8.5
    • Glycerol 5 v/v -
    		pH 8.5
    100 μl of Glycerol (50 v/v)
    100 μl of TABS pH 8.5 (1 M)
    800 μl of water (100 v/v)
    F8
    • TAPS 0.1 M pH 8.5
    • Glycerol 5 v/v -
    		pH 8.5
    100 μl of Glycerol (50 v/v)
    100 μl of TAPS pH 8.5 (1 M)
    800 μl of water (100 v/v)
    F9
    • CAPSO 0.1 M pH 9
    • Glycerol 5 v/v -
    		pH 9
    100 μl of CAPSO pH 9 (1 M)
    100 μl of Glycerol (50 v/v)
    800 μl of water (100 v/v)
    F10
    • CHES 0.1 M pH 9
    • Glycerol 5 v/v -
    		pH 9
    100 μl of Glycerol (50 v/v)
    100 μl of CHES pH 9 (1 M)
    800 μl of water (100 v/v)
    F11
    • Glycine 0.1 M pH 9.5
    • Glycerol 5 v/v -
    		pH 9.5
    100 μl of Glycerol (50 v/v)
    50 μl of Glycine pH 9.5 (2 M)
    850 μl of water (100 v/v)
    F12
    • CAPS 0.1 M pH 10
    • Glycerol 5 v/v -
    		pH 10
    100 μl of Glycerol (50 v/v)
    100 μl of CAPS pH 10 (1 M)
    800 μl of water (100 v/v)
    G1
    • Glycerol 5 v/v -
    • Sodium chloride 0.15 M -
    • Potassium acetate 0.1 M pH 5
    		pH 5
    30 μl of Sodium chloride (5 M)
    100 μl of Glycerol (50 v/v)
    100 μl of Potassium acetate pH 5 (1 M)
    770 μl of water (100 v/v)
    G2
    • Glycerol 5 v/v -
    • Sodium chloride 0.15 M -
    • MES 0.1 M pH 5.5
    		pH 5.5
    30 μl of Sodium chloride (5 M)
    100 μl of Glycerol (50 v/v)
    100 μl of MES pH 5.5 (1 M)
    770 μl of water (100 v/v)
    G3
    • Glycerol 5 v/v -
    • Sodium chloride 0.15 M -
    • ADA 0.1 M pH 6
    		pH 6
    30 μl of Sodium chloride (5 M)
    100 μl of Glycerol (50 v/v)
    100 μl of ADA pH 6 (1 M)
    770 μl of water (100 v/v)
    G4
    • Glycerol 5 v/v -
    • Sodium chloride 0.15 M -
    • MES 0.1 M pH 6.5
    		pH 6.5
    30 μl of Sodium chloride (5 M)
    100 μl of Glycerol (50 v/v)
    100 μl of MES pH 6.5 (1 M)
    770 μl of water (100 v/v)
    G5
    • Glycerol 5 v/v -
    • Sodium chloride 0.15 M -
    • BIS-TRIS 0.1 M pH 6.5
    		pH 6.5
    30 μl of Sodium chloride (5 M)
    100 μl of Glycerol (50 v/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    770 μl of water (100 v/v)
    G6
    • Glycerol 5 v/v -
    • Sodium chloride 0.15 M -
    • di-Potassium hydrogen phosphate 0.1 M pH 6.5
    		pH 6.5
    50 μl of di-Potassium hydrogen phosphate pH 6.5 (2 M)
    30 μl of Sodium chloride (5 M)
    100 μl of Glycerol (50 v/v)
    820 μl of water (100 v/v)
    G7
    • Glycerol 5 v/v -
    • Sodium chloride 0.15 M -
    • PIPES 0.1 M pH 7
    		pH 7
    30 μl of Sodium chloride (5 M)
    100 μl of PIPES pH 7 (1 M)
    100 μl of Glycerol (50 v/v)
    770 μl of water (100 v/v)
    G8
    • Glycerol 5 v/v -
    • Sodium chloride 0.15 M -
    • Imidazole 0.1 M pH 7
    		pH 7
    30 μl of Sodium chloride (5 M)
    100 μl of Glycerol (50 v/v)
    100 μl of Imidazole pH 7 (1 M)
    770 μl of water (100 v/v)
    G9
    • Glycerol 5 v/v -
    • Sodium chloride 0.15 M -
    • ADA 0.1 M pH 7
    		pH 7
    30 μl of Sodium chloride (5 M)
    100 μl of Glycerol (50 v/v)
    100 μl of ADA pH 7 (1 M)
    770 μl of water (100 v/v)
    G10
    • Glycerol 5 v/v -
    • Sodium chloride 0.15 M -
    • MOPS 0.1 M pH 7
    		pH 7
    30 μl of Sodium chloride (5 M)
    100 μl of Glycerol (50 v/v)
    100 μl of MOPS pH 7 (1 M)
    770 μl of water (100 v/v)
    G11
    • Glycerol 5 v/v -
    • Sodium chloride 0.15 M -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    30 μl of Sodium chloride (5 M)
    100 μl of Glycerol (50 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    770 μl of water (100 v/v)
    G12
    • Glycerol 5 v/v -
    • Sodium chloride 0.15 M -
    • di-Potassium hydrogen phosphate 0.1 M pH 7.5
    		pH 7.5
    100 μl of di-Potassium hydrogen phosphate pH 7.5 (1 M)
    30 μl of Sodium chloride (5 M)
    100 μl of Glycerol (50 v/v)
    770 μl of water (100 v/v)
    H1
    • Glycerol 5 v/v -
    • Sodium chloride 0.15 M -
    • DIPSO 0.1 M pH 7.5
    		pH 7.5
    100 μl of DIPSO pH 7.5 (1 M)
    30 μl of Sodium chloride (5 M)
    100 μl of Glycerol (50 v/v)
    770 μl of water (100 v/v)
    H2
    • Glycerol 5 v/v -
    • Sodium chloride 0.15 M -
    • TRICINE 0.1 M pH 7.5
    		pH 7.5
    100 μl of TRICINE pH 7.5 (1 M)
    30 μl of Sodium chloride (5 M)
    100 μl of Glycerol (50 v/v)
    770 μl of water (100 v/v)
    H3
    • Glycerol 5 v/v -
    • Sodium chloride 0.15 M -
    • HEPES 0.1 M pH 8
    		pH 8
    100 μl of HEPES pH 8 (1 M)
    30 μl of Sodium chloride (5 M)
    100 μl of Glycerol (50 v/v)
    770 μl of water (100 v/v)
    H4
    • Glycerol 5 v/v -
    • Sodium chloride 0.15 M -
    • BICINE 0.1 M pH 8
    		pH 8
    30 μl of Sodium chloride (5 M)
    100 μl of Glycerol (50 v/v)
    100 μl of BICINE pH 8 (1 M)
    770 μl of water (100 v/v)
    H5
    • Glycerol 5 v/v -
    • Sodium chloride 0.15 M -
    • tris chloride BCC 0.1 M pH 8
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    30 μl of Sodium chloride (5 M)
    100 μl of Glycerol (50 v/v)
    770 μl of water (100 v/v)
    H6
    • Glycerol 5 v/v -
    • Sodium chloride 0.15 M -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    30 μl of Sodium chloride (5 M)
    100 μl of Glycerol (50 v/v)
    770 μl of water (100 v/v)
    H7
    • Glycerol 5 v/v -
    • Sodium chloride 0.15 M -
    • TABS 0.1 M pH 8.5
    		pH 8.5
    30 μl of Sodium chloride (5 M)
    100 μl of Glycerol (50 v/v)
    100 μl of TABS pH 8.5 (1 M)
    770 μl of water (100 v/v)
    H8
    • Glycerol 5 v/v -
    • Sodium chloride 0.15 M -
    • TAPS 0.1 M pH 8.5
    		pH 8.5
    30 μl of Sodium chloride (5 M)
    100 μl of Glycerol (50 v/v)
    100 μl of TAPS pH 8.5 (1 M)
    770 μl of water (100 v/v)
    H9
    • Glycerol 5 v/v -
    • Sodium chloride 0.15 M -
    • CAPSO 0.1 M pH 9
    		pH 9
    100 μl of CAPSO pH 9 (1 M)
    30 μl of Sodium chloride (5 M)
    100 μl of Glycerol (50 v/v)
    770 μl of water (100 v/v)
    H10
    • Glycerol 5 v/v -
    • Sodium chloride 0.15 M -
    • CHES 0.1 M pH 9
    		pH 9
    30 μl of Sodium chloride (5 M)
    100 μl of Glycerol (50 v/v)
    100 μl of CHES pH 9 (1 M)
    770 μl of water (100 v/v)
    H11
    • Glycerol 5 v/v -
    • Sodium chloride 0.15 M -
    • Glycine 0.1 M pH 9.5
    		pH 9.5
    30 μl of Sodium chloride (5 M)
    100 μl of Glycerol (50 v/v)
    50 μl of Glycine pH 9.5 (2 M)
    820 μl of water (100 v/v)
    H12NA
    check warnings