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Supplemental Information

Screen Description

Solubility_HTS

Optimization / Solubility_HTS
Created 2013-03-25.

Export

Solubility_HTS

Barcodes

Solubility_HTS

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Screen Structure

Solubility_HTS

Well Count 96
Number of distinct ingredients 22
Internal diversity (score mean dist) 0.847

Screen Composition

More information about screen composition chart.
2025-08-05T18:12:41.510231 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
ingredient type Solubility_HTS
Buffer 94
Cryoprotectant 47
Organic (non-volatile) 47
Salt 58

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Solubility_HTS Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Acetonitrile 20 v/v -
NA
1000.0 μl of Acetonitrile (20 v/v)
A2
  • MES 0.1 M pH 5.5
pH 5.5
100.0 μl of MES pH 5.5 (1 M)
900.0 μl of water (100 v/v)
A3
  • ADA 0.1 M pH 6
pH 6
100.0 μl of ADA pH 6 (1 M)
900.0 μl of water (100 v/v)
A4
  • MES 0.1 M pH 6.5
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
900.0 μl of water (100 v/v)
A5
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
900.0 μl of water (100 v/v)
A6
  • di-Potassium hydrogen phosphate 0.1 M pH 6.5
pH 6.5
50.0 μl of di-Potassium hydrogen phosphate pH 6.5 (2 M)
950.0 μl of water (100 v/v)
A7
  • PIPES 0.1 M pH 7
pH 7
100.0 μl of PIPES pH 7 (1 M)
900.0 μl of water (100 v/v)
A8
  • Imidazole 0.1 M pH 7
pH 7
100.0 μl of Imidazole pH 7 (1 M)
900.0 μl of water (100 v/v)
A9
  • ADA 0.1 M pH 7
pH 7
100.0 μl of ADA pH 7 (1 M)
900.0 μl of water (100 v/v)
A10
  • MOPS 0.1 M pH 7
pH 7
100.0 μl of MOPS pH 7 (1 M)
900.0 μl of water (100 v/v)
A11
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
900.0 μl of water (100 v/v)
A12
  • di-Potassium hydrogen phosphate 0.1 M pH 7.5
pH 7.5
100.0 μl of di-Potassium hydrogen phosphate pH 7.5 (1 M)
900.0 μl of water (100 v/v)
B1
  • DIPSO 0.1 M pH 7.5
pH 7.5
100.0 μl of DIPSO pH 7.5 (1 M)
900.0 μl of water (100 v/v)
B2
  • TRICINE 0.1 M pH 7.5
pH 7.5
100.0 μl of TRICINE pH 7.5 (1 M)
900.0 μl of water (100 v/v)
B3
  • HEPES 0.1 M pH 8
pH 8
100.0 μl of HEPES pH 8 (1 M)
900.0 μl of water (100 v/v)
B4
  • BICINE 0.1 M pH 8
pH 8
100.0 μl of BICINE pH 8 (1 M)
900.0 μl of water (100 v/v)
B5
  • tris chloride BCC 0.1 M pH 8
pH 8
100.0 μl of tris chloride BCC pH 8 (1 M)
900.0 μl of water (100 v/v)
B6
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
100.0 μl of tris chloride BCC pH 8.5 (1 M)
900.0 μl of water (100 v/v)
B7
  • TABS 0.1 M pH 8.5
pH 8.5
100.0 μl of TABS pH 8.5 (1 M)
900.0 μl of water (100 v/v)
B8
  • TAPS 0.1 M pH 8.5
pH 8.5
100.0 μl of TAPS pH 8.5 (1 M)
900.0 μl of water (100 v/v)
B9
  • CAPSO 0.1 M pH 9
pH 9
100.0 μl of CAPSO pH 9 (1 M)
900.0 μl of water (100 v/v)
B10
  • CHES 0.1 M pH 9
pH 9
100.0 μl of CHES pH 9 (1 M)
900.0 μl of water (100 v/v)
B11
  • Glycine 0.1 M pH 9.5
pH 9.5
50.0 μl of Glycine pH 9.5 (2 M)
950.0 μl of water (100 v/v)
B12
  • CAPS 0.1 M pH 10
pH 10
100.0 μl of CAPS pH 10 (1 M)
900.0 μl of water (100 v/v)
C1
  • Potassium acetate 0.1 M pH 5
  • Sodium chloride 0.15 M -
pH 5
100.0 μl of Potassium acetate pH 5 (1 M)
30.0 μl of Sodium chloride (5 M)
870.0 μl of water (100 v/v)
C2
  • MES 0.1 M pH 5.5
  • Sodium chloride 0.15 M -
pH 5.5
100.0 μl of MES pH 5.5 (1 M)
30.0 μl of Sodium chloride (5 M)
870.0 μl of water (100 v/v)
C3
  • ADA 0.1 M pH 6
  • Sodium chloride 0.15 M -
pH 6
100.0 μl of ADA pH 6 (1 M)
30.0 μl of Sodium chloride (5 M)
870.0 μl of water (100 v/v)
C4
  • MES 0.1 M pH 6.5
  • Sodium chloride 0.15 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
30.0 μl of Sodium chloride (5 M)
870.0 μl of water (100 v/v)
C5
  • BIS-TRIS 0.1 M pH 6.5
  • Sodium chloride 0.15 M -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
30.0 μl of Sodium chloride (5 M)
870.0 μl of water (100 v/v)
C6
  • di-Potassium hydrogen phosphate 0.1 M pH 6.5
  • Sodium chloride 0.15 M -
pH 6.5
30.0 μl of Sodium chloride (5 M)
50.0 μl of di-Potassium hydrogen phosphate pH 6.5 (2 M)
920.0 μl of water (100 v/v)
C7
  • PIPES 0.1 M pH 7
  • Sodium chloride 0.15 M -
pH 7
100.0 μl of PIPES pH 7 (1 M)
30.0 μl of Sodium chloride (5 M)
870.0 μl of water (100 v/v)
C8
  • Imidazole 0.1 M pH 7
  • Sodium chloride 0.15 M -
pH 7
100.0 μl of Imidazole pH 7 (1 M)
30.0 μl of Sodium chloride (5 M)
870.0 μl of water (100 v/v)
C9
  • ADA 0.1 M pH 7
  • Sodium chloride 0.15 M -
pH 7
100.0 μl of ADA pH 7 (1 M)
30.0 μl of Sodium chloride (5 M)
870.0 μl of water (100 v/v)
C10
  • MOPS 0.1 M pH 7
  • Sodium chloride 0.15 M -
pH 7
100.0 μl of MOPS pH 7 (1 M)
30.0 μl of Sodium chloride (5 M)
870.0 μl of water (100 v/v)
C11
  • HEPES 0.1 M pH 7.5
  • Sodium chloride 0.15 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
30.0 μl of Sodium chloride (5 M)
870.0 μl of water (100 v/v)
C12
  • di-Potassium hydrogen phosphate 0.1 M pH 7.5
  • Sodium chloride 0.15 M -
pH 7.5
30.0 μl of Sodium chloride (5 M)
100.0 μl of di-Potassium hydrogen phosphate pH 7.5 (1 M)
870.0 μl of water (100 v/v)
D1
  • DIPSO 0.1 M pH 7.5
  • Sodium chloride 0.15 M -
pH 7.5
100.0 μl of DIPSO pH 7.5 (1 M)
30.0 μl of Sodium chloride (5 M)
870.0 μl of water (100 v/v)
D2
  • TRICINE 0.1 M pH 7.5
  • Sodium chloride 0.15 M -
pH 7.5
30.0 μl of Sodium chloride (5 M)
100.0 μl of TRICINE pH 7.5 (1 M)
870.0 μl of water (100 v/v)
D3
  • HEPES 0.1 M pH 8
  • Sodium chloride 0.15 M -
pH 8
100.0 μl of HEPES pH 8 (1 M)
30.0 μl of Sodium chloride (5 M)
870.0 μl of water (100 v/v)
D4
  • BICINE 0.1 M pH 8
  • Sodium chloride 0.15 M -
pH 8
100.0 μl of BICINE pH 8 (1 M)
30.0 μl of Sodium chloride (5 M)
870.0 μl of water (100 v/v)
D5
  • tris chloride BCC 0.1 M pH 8
  • Sodium chloride 0.15 M -
pH 8
30.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
870.0 μl of water (100 v/v)
D6
  • tris chloride BCC 0.1 M pH 8.5
  • Sodium chloride 0.15 M -
pH 8.5
30.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
870.0 μl of water (100 v/v)
D7
  • TABS 0.1 M pH 8.5
  • Sodium chloride 0.15 M -
pH 8.5
30.0 μl of Sodium chloride (5 M)
100.0 μl of TABS pH 8.5 (1 M)
870.0 μl of water (100 v/v)
D8
  • TAPS 0.1 M pH 8.5
  • Sodium chloride 0.15 M -
pH 8.5
30.0 μl of Sodium chloride (5 M)
100.0 μl of TAPS pH 8.5 (1 M)
870.0 μl of water (100 v/v)
D9
  • CAPSO 0.1 M pH 9
  • Sodium chloride 0.15 M -
pH 9
100.0 μl of CAPSO pH 9 (1 M)
30.0 μl of Sodium chloride (5 M)
870.0 μl of water (100 v/v)
D10
  • CHES 0.1 M pH 9
  • Sodium chloride 0.15 M -
pH 9
100.0 μl of CHES pH 9 (1 M)
30.0 μl of Sodium chloride (5 M)
870.0 μl of water (100 v/v)
D11
  • Glycine 0.1 M pH 9.5
  • Sodium chloride 0.15 M -
pH 9.5
50.0 μl of Glycine pH 9.5 (2 M)
30.0 μl of Sodium chloride (5 M)
920.0 μl of water (100 v/v)
D12
  • CAPS 0.1 M pH 10
  • Sodium chloride 0.15 M -
pH 10
100.0 μl of CAPS pH 10 (1 M)
30.0 μl of Sodium chloride (5 M)
870.0 μl of water (100 v/v)
E1
  • Potassium acetate 0.1 M pH 5
  • Glycerol 5 v/v -
pH 5
100.0 μl of Glycerol (50 v/v)
100.0 μl of Potassium acetate pH 5 (1 M)
800.0 μl of water (100 v/v)
E2
  • MES 0.1 M pH 5.5
  • Glycerol 5 v/v -
pH 5.5
100.0 μl of Glycerol (50 v/v)
100.0 μl of MES pH 5.5 (1 M)
800.0 μl of water (100 v/v)
E3
  • ADA 0.1 M pH 6
  • Glycerol 5 v/v -
pH 6
100.0 μl of ADA pH 6 (1 M)
100.0 μl of Glycerol (50 v/v)
800.0 μl of water (100 v/v)
E4
  • MES 0.1 M pH 6.5
  • Glycerol 5 v/v -
pH 6.5
100.0 μl of Glycerol (50 v/v)
100.0 μl of MES pH 6.5 (1 M)
800.0 μl of water (100 v/v)
E5
  • BIS-TRIS 0.1 M pH 6.5
  • Glycerol 5 v/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
100.0 μl of Glycerol (50 v/v)
800.0 μl of water (100 v/v)
E6
  • di-Potassium hydrogen phosphate 0.1 M pH 6.5
  • Glycerol 5 v/v -
pH 6.5
100.0 μl of Glycerol (50 v/v)
50.0 μl of di-Potassium hydrogen phosphate pH 6.5 (2 M)
850.0 μl of water (100 v/v)
E7
  • PIPES 0.1 M pH 7
  • Glycerol 5 v/v -
pH 7
100.0 μl of Glycerol (50 v/v)
100.0 μl of PIPES pH 7 (1 M)
800.0 μl of water (100 v/v)
E8
  • Imidazole 0.1 M pH 7
  • Glycerol 5 v/v -
pH 7
100.0 μl of Glycerol (50 v/v)
100.0 μl of Imidazole pH 7 (1 M)
800.0 μl of water (100 v/v)
E9
  • ADA 0.1 M pH 7
  • Glycerol 5 v/v -
pH 7
100.0 μl of ADA pH 7 (1 M)
100.0 μl of Glycerol (50 v/v)
800.0 μl of water (100 v/v)
E10
  • MOPS 0.1 M pH 7
  • Glycerol 5 v/v -
pH 7
100.0 μl of Glycerol (50 v/v)
100.0 μl of MOPS pH 7 (1 M)
800.0 μl of water (100 v/v)
E11
  • HEPES 0.1 M pH 7.5
  • Glycerol 5 v/v -
pH 7.5
100.0 μl of Glycerol (50 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
800.0 μl of water (100 v/v)
E12
  • di-Potassium hydrogen phosphate 0.1 M pH 7.5
  • Glycerol 5 v/v -
pH 7.5
100.0 μl of Glycerol (50 v/v)
100.0 μl of di-Potassium hydrogen phosphate pH 7.5 (1 M)
800.0 μl of water (100 v/v)
F1
  • DIPSO 0.1 M pH 7.5
  • Glycerol 5 v/v -
pH 7.5
100.0 μl of DIPSO pH 7.5 (1 M)
100.0 μl of Glycerol (50 v/v)
800.0 μl of water (100 v/v)
F2
  • TRICINE 0.1 M pH 7.5
  • Glycerol 5 v/v -
pH 7.5
100.0 μl of Glycerol (50 v/v)
100.0 μl of TRICINE pH 7.5 (1 M)
800.0 μl of water (100 v/v)
F3
  • HEPES 0.1 M pH 8
  • Glycerol 5 v/v -
pH 8
100.0 μl of Glycerol (50 v/v)
100.0 μl of HEPES pH 8 (1 M)
800.0 μl of water (100 v/v)
F4
  • BICINE 0.1 M pH 8
  • Glycerol 5 v/v -
pH 8
100.0 μl of BICINE pH 8 (1 M)
100.0 μl of Glycerol (50 v/v)
800.0 μl of water (100 v/v)
F5
  • tris chloride BCC 0.1 M pH 8
  • Glycerol 5 v/v -
pH 8
100.0 μl of Glycerol (50 v/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
800.0 μl of water (100 v/v)
F6
  • tris chloride BCC 0.1 M pH 8.5
  • Glycerol 5 v/v -
pH 8.5
100.0 μl of Glycerol (50 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
800.0 μl of water (100 v/v)
F7
  • TABS 0.1 M pH 8.5
  • Glycerol 5 v/v -
pH 8.5
100.0 μl of Glycerol (50 v/v)
100.0 μl of TABS pH 8.5 (1 M)
800.0 μl of water (100 v/v)
F8
  • TAPS 0.1 M pH 8.5
  • Glycerol 5 v/v -
pH 8.5
100.0 μl of Glycerol (50 v/v)
100.0 μl of TAPS pH 8.5 (1 M)
800.0 μl of water (100 v/v)
F9
  • CAPSO 0.1 M pH 9
  • Glycerol 5 v/v -
pH 9
100.0 μl of CAPSO pH 9 (1 M)
100.0 μl of Glycerol (50 v/v)
800.0 μl of water (100 v/v)
F10
  • CHES 0.1 M pH 9
  • Glycerol 5 v/v -
pH 9
100.0 μl of CHES pH 9 (1 M)
100.0 μl of Glycerol (50 v/v)
800.0 μl of water (100 v/v)
F11
  • Glycine 0.1 M pH 9.5
  • Glycerol 5 v/v -
pH 9.5
100.0 μl of Glycerol (50 v/v)
50.0 μl of Glycine pH 9.5 (2 M)
850.0 μl of water (100 v/v)
F12
  • CAPS 0.1 M pH 10
  • Glycerol 5 v/v -
pH 10
100.0 μl of CAPS pH 10 (1 M)
100.0 μl of Glycerol (50 v/v)
800.0 μl of water (100 v/v)
G1
  • Glycerol 5 v/v -
  • Sodium chloride 0.15 M -
  • Potassium acetate 0.1 M pH 5
pH 5
100.0 μl of Glycerol (50 v/v)
100.0 μl of Potassium acetate pH 5 (1 M)
30.0 μl of Sodium chloride (5 M)
770.0 μl of water (100 v/v)
G2
  • Glycerol 5 v/v -
  • Sodium chloride 0.15 M -
  • MES 0.1 M pH 5.5
pH 5.5
100.0 μl of Glycerol (50 v/v)
100.0 μl of MES pH 5.5 (1 M)
30.0 μl of Sodium chloride (5 M)
770.0 μl of water (100 v/v)
G3
  • Glycerol 5 v/v -
  • Sodium chloride 0.15 M -
  • ADA 0.1 M pH 6
pH 6
100.0 μl of ADA pH 6 (1 M)
100.0 μl of Glycerol (50 v/v)
30.0 μl of Sodium chloride (5 M)
770.0 μl of water (100 v/v)
G4
  • Glycerol 5 v/v -
  • Sodium chloride 0.15 M -
  • MES 0.1 M pH 6.5
pH 6.5
100.0 μl of Glycerol (50 v/v)
100.0 μl of MES pH 6.5 (1 M)
30.0 μl of Sodium chloride (5 M)
770.0 μl of water (100 v/v)
G5
  • Glycerol 5 v/v -
  • Sodium chloride 0.15 M -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
100.0 μl of Glycerol (50 v/v)
30.0 μl of Sodium chloride (5 M)
770.0 μl of water (100 v/v)
G6
  • Glycerol 5 v/v -
  • Sodium chloride 0.15 M -
  • di-Potassium hydrogen phosphate 0.1 M pH 6.5
pH 6.5
100.0 μl of Glycerol (50 v/v)
30.0 μl of Sodium chloride (5 M)
50.0 μl of di-Potassium hydrogen phosphate pH 6.5 (2 M)
820.0 μl of water (100 v/v)
G7
  • Glycerol 5 v/v -
  • Sodium chloride 0.15 M -
  • PIPES 0.1 M pH 7
pH 7
100.0 μl of Glycerol (50 v/v)
100.0 μl of PIPES pH 7 (1 M)
30.0 μl of Sodium chloride (5 M)
770.0 μl of water (100 v/v)
G8
  • Glycerol 5 v/v -
  • Sodium chloride 0.15 M -
  • Imidazole 0.1 M pH 7
pH 7
100.0 μl of Glycerol (50 v/v)
100.0 μl of Imidazole pH 7 (1 M)
30.0 μl of Sodium chloride (5 M)
770.0 μl of water (100 v/v)
G9
  • Glycerol 5 v/v -
  • Sodium chloride 0.15 M -
  • ADA 0.1 M pH 7
pH 7
100.0 μl of ADA pH 7 (1 M)
100.0 μl of Glycerol (50 v/v)
30.0 μl of Sodium chloride (5 M)
770.0 μl of water (100 v/v)
G10
  • Glycerol 5 v/v -
  • Sodium chloride 0.15 M -
  • MOPS 0.1 M pH 7
pH 7
100.0 μl of Glycerol (50 v/v)
100.0 μl of MOPS pH 7 (1 M)
30.0 μl of Sodium chloride (5 M)
770.0 μl of water (100 v/v)
G11
  • Glycerol 5 v/v -
  • Sodium chloride 0.15 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of Glycerol (50 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
30.0 μl of Sodium chloride (5 M)
770.0 μl of water (100 v/v)
G12
  • Glycerol 5 v/v -
  • Sodium chloride 0.15 M -
  • di-Potassium hydrogen phosphate 0.1 M pH 7.5
pH 7.5
100.0 μl of Glycerol (50 v/v)
30.0 μl of Sodium chloride (5 M)
100.0 μl of di-Potassium hydrogen phosphate pH 7.5 (1 M)
770.0 μl of water (100 v/v)
H1
  • Glycerol 5 v/v -
  • Sodium chloride 0.15 M -
  • DIPSO 0.1 M pH 7.5
pH 7.5
100.0 μl of DIPSO pH 7.5 (1 M)
100.0 μl of Glycerol (50 v/v)
30.0 μl of Sodium chloride (5 M)
770.0 μl of water (100 v/v)
H2
  • Glycerol 5 v/v -
  • Sodium chloride 0.15 M -
  • TRICINE 0.1 M pH 7.5
pH 7.5
100.0 μl of Glycerol (50 v/v)
30.0 μl of Sodium chloride (5 M)
100.0 μl of TRICINE pH 7.5 (1 M)
770.0 μl of water (100 v/v)
H3
  • Glycerol 5 v/v -
  • Sodium chloride 0.15 M -
  • HEPES 0.1 M pH 8
pH 8
100.0 μl of Glycerol (50 v/v)
100.0 μl of HEPES pH 8 (1 M)
30.0 μl of Sodium chloride (5 M)
770.0 μl of water (100 v/v)
H4
  • Glycerol 5 v/v -
  • Sodium chloride 0.15 M -
  • BICINE 0.1 M pH 8
pH 8
100.0 μl of BICINE pH 8 (1 M)
100.0 μl of Glycerol (50 v/v)
30.0 μl of Sodium chloride (5 M)
770.0 μl of water (100 v/v)
H5
  • Glycerol 5 v/v -
  • Sodium chloride 0.15 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
100.0 μl of Glycerol (50 v/v)
30.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
770.0 μl of water (100 v/v)
H6
  • Glycerol 5 v/v -
  • Sodium chloride 0.15 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
100.0 μl of Glycerol (50 v/v)
30.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
770.0 μl of water (100 v/v)
H7
  • Glycerol 5 v/v -
  • Sodium chloride 0.15 M -
  • TABS 0.1 M pH 8.5
pH 8.5
100.0 μl of Glycerol (50 v/v)
30.0 μl of Sodium chloride (5 M)
100.0 μl of TABS pH 8.5 (1 M)
770.0 μl of water (100 v/v)
H8
  • Glycerol 5 v/v -
  • Sodium chloride 0.15 M -
  • TAPS 0.1 M pH 8.5
pH 8.5
100.0 μl of Glycerol (50 v/v)
30.0 μl of Sodium chloride (5 M)
100.0 μl of TAPS pH 8.5 (1 M)
770.0 μl of water (100 v/v)
H9
  • Glycerol 5 v/v -
  • Sodium chloride 0.15 M -
  • CAPSO 0.1 M pH 9
pH 9
100.0 μl of CAPSO pH 9 (1 M)
100.0 μl of Glycerol (50 v/v)
30.0 μl of Sodium chloride (5 M)
770.0 μl of water (100 v/v)
H10
  • Glycerol 5 v/v -
  • Sodium chloride 0.15 M -
  • CHES 0.1 M pH 9
pH 9
100.0 μl of CHES pH 9 (1 M)
100.0 μl of Glycerol (50 v/v)
30.0 μl of Sodium chloride (5 M)
770.0 μl of water (100 v/v)
H11
  • Glycerol 5 v/v -
  • Sodium chloride 0.15 M -
  • Glycine 0.1 M pH 9.5
pH 9.5
100.0 μl of Glycerol (50 v/v)
50.0 μl of Glycine pH 9.5 (2 M)
30.0 μl of Sodium chloride (5 M)
820.0 μl of water (100 v/v)
H12NA
1000.0 μl of water (100 v/v)