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Supplemental Information

Screen Description

GRAS6

GRAS screens / GRAS6
Created 2016-09-29.

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Barcodes

GRAS6

No barcodes found.

Screen Structure

GRAS6

Well Count 96
Number of distinct ingredients 12
Internal diversity (score mean dist) 0.895

Screen Composition

2025-08-11T22:33:09.639739 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type GRAS6
Buffer 96
Polymer 96
Salt 36

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
GRAS6 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 1,000 4 w/v -
pH 4.5
80.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
820.0 μl of water (100 v/v)
A2
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 1,000 16 w/v -
pH 4.5
320.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
580.0 μl of water (100 v/v)
A3
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 1,000 28 w/v -
pH 4.5
560.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
340.0 μl of water (100 v/v)
A4
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol monomethyl ether 2,000 4 w/v -
pH 4.5
80.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
820.0 μl of water (100 v/v)
A5
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol monomethyl ether 2,000 16 w/v -
pH 4.5
320.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
580.0 μl of water (100 v/v)
A6
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol monomethyl ether 2,000 28 w/v -
pH 4.5
560.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
340.0 μl of water (100 v/v)
A7
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 3,350 4 w/v -
pH 4.5
80.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
820.0 μl of water (100 v/v)
A8
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 3,350 16 w/v -
pH 4.5
320.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
580.0 μl of water (100 v/v)
A9
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 3,350 28 w/v -
pH 4.5
560.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
340.0 μl of water (100 v/v)
A10
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 4,000 4 w/v -
pH 4.5
80.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
820.0 μl of water (100 v/v)
A11
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 4,000 16 w/v -
pH 4.5
320.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
580.0 μl of water (100 v/v)
A12
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 4,000 28 w/v -
pH 4.5
560.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
340.0 μl of water (100 v/v)
B1
  • di-Sodium succinate 0.1 M pH 5.5
  • Polyethylene glycol 1,000 4 w/v -
pH 5.5
80.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of di-Sodium succinate pH 5.5 (1 M)
820.0 μl of water (100 v/v)
B2
  • di-Sodium succinate 0.1 M pH 5.5
  • Polyethylene glycol 1,000 16 w/v -
pH 5.5
320.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of di-Sodium succinate pH 5.5 (1 M)
580.0 μl of water (100 v/v)
B3
  • di-Sodium succinate 0.1 M pH 5.5
  • Polyethylene glycol 1,000 28 w/v -
pH 5.5
560.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of di-Sodium succinate pH 5.5 (1 M)
340.0 μl of water (100 v/v)
B4
  • di-Sodium succinate 0.1 M pH 5.5
  • Polyethylene glycol monomethyl ether 2,000 4 w/v -
pH 5.5
80.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of di-Sodium succinate pH 5.5 (1 M)
820.0 μl of water (100 v/v)
B5
  • di-Sodium succinate 0.1 M pH 5.5
  • Polyethylene glycol monomethyl ether 2,000 16 w/v -
pH 5.5
320.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of di-Sodium succinate pH 5.5 (1 M)
580.0 μl of water (100 v/v)
B6
  • di-Sodium succinate 0.1 M pH 5.5
  • Polyethylene glycol monomethyl ether 2,000 28 w/v -
pH 5.5
560.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of di-Sodium succinate pH 5.5 (1 M)
340.0 μl of water (100 v/v)
B7
  • di-Sodium succinate 0.1 M pH 5.5
  • Polyethylene glycol 3,350 4 w/v -
pH 5.5
80.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of di-Sodium succinate pH 5.5 (1 M)
820.0 μl of water (100 v/v)
B8
  • di-Sodium succinate 0.1 M pH 5.5
  • Polyethylene glycol 3,350 16 w/v -
pH 5.5
320.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of di-Sodium succinate pH 5.5 (1 M)
580.0 μl of water (100 v/v)
B9
  • di-Sodium succinate 0.1 M pH 5.5
  • Polyethylene glycol 3,350 28 w/v -
pH 5.5
560.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of di-Sodium succinate pH 5.5 (1 M)
340.0 μl of water (100 v/v)
B10
  • di-Sodium succinate 0.1 M pH 5.5
  • Polyethylene glycol 4,000 4 w/v -
pH 5.5
80.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of di-Sodium succinate pH 5.5 (1 M)
820.0 μl of water (100 v/v)
B11
  • di-Sodium succinate 0.1 M pH 5.5
  • Polyethylene glycol 4,000 16 w/v -
pH 5.5
320.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of di-Sodium succinate pH 5.5 (1 M)
580.0 μl of water (100 v/v)
B12
  • di-Sodium succinate 0.1 M pH 5.5
  • Polyethylene glycol 4,000 28 w/v -
pH 5.5
560.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of di-Sodium succinate pH 5.5 (1 M)
340.0 μl of water (100 v/v)
C1
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 1,000 4 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
80.0 μl of Polyethylene glycol 1,000 (50 w/v)
820.0 μl of water (100 v/v)
C2
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 1,000 16 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
320.0 μl of Polyethylene glycol 1,000 (50 w/v)
580.0 μl of water (100 v/v)
C3
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 1,000 28 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
560.0 μl of Polyethylene glycol 1,000 (50 w/v)
340.0 μl of water (100 v/v)
C4
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 2,000 4 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
80.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
820.0 μl of water (100 v/v)
C5
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 2,000 16 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
320.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
580.0 μl of water (100 v/v)
C6
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 2,000 28 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
560.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
340.0 μl of water (100 v/v)
C7
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 3,350 4 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
80.0 μl of Polyethylene glycol 3,350 (50 w/v)
820.0 μl of water (100 v/v)
C8
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 3,350 16 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
320.0 μl of Polyethylene glycol 3,350 (50 w/v)
580.0 μl of water (100 v/v)
C9
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 3,350 28 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
560.0 μl of Polyethylene glycol 3,350 (50 w/v)
340.0 μl of water (100 v/v)
C10
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 4,000 4 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
80.0 μl of Polyethylene glycol 4,000 (50 w/v)
820.0 μl of water (100 v/v)
C11
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 4,000 16 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
320.0 μl of Polyethylene glycol 4,000 (50 w/v)
580.0 μl of water (100 v/v)
C12
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 4,000 28 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
560.0 μl of Polyethylene glycol 4,000 (50 w/v)
340.0 μl of water (100 v/v)
D1
  • Sodium Potassium phosphate 0.1 M pH 7
  • Polyethylene glycol 1,000 4 w/v -
pH 7
80.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of Sodium Potassium phosphate pH 7 (1 M)
820.0 μl of water (100 v/v)
D2
  • Sodium Potassium phosphate 0.1 M pH 7
  • Polyethylene glycol 1,000 16 w/v -
pH 7
320.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of Sodium Potassium phosphate pH 7 (1 M)
580.0 μl of water (100 v/v)
D3
  • Sodium Potassium phosphate 0.1 M pH 7
  • Polyethylene glycol 1,000 28 w/v -
pH 7
560.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of Sodium Potassium phosphate pH 7 (1 M)
340.0 μl of water (100 v/v)
D4
  • Sodium Potassium phosphate 0.1 M pH 7
  • Polyethylene glycol monomethyl ether 2,000 4 w/v -
pH 7
80.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium Potassium phosphate pH 7 (1 M)
820.0 μl of water (100 v/v)
D5
  • Sodium Potassium phosphate 0.1 M pH 7
  • Polyethylene glycol monomethyl ether 2,000 16 w/v -
pH 7
320.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium Potassium phosphate pH 7 (1 M)
580.0 μl of water (100 v/v)
D6
  • Sodium Potassium phosphate 0.1 M pH 7
  • Polyethylene glycol monomethyl ether 2,000 28 w/v -
pH 7
560.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium Potassium phosphate pH 7 (1 M)
340.0 μl of water (100 v/v)
D7
  • Sodium Potassium phosphate 0.1 M pH 7
  • Polyethylene glycol 3,350 4 w/v -
pH 7
80.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium Potassium phosphate pH 7 (1 M)
820.0 μl of water (100 v/v)
D8
  • Sodium Potassium phosphate 0.1 M pH 7
  • Polyethylene glycol 3,350 16 w/v -
pH 7
320.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium Potassium phosphate pH 7 (1 M)
580.0 μl of water (100 v/v)
D9
  • Sodium Potassium phosphate 0.1 M pH 7
  • Polyethylene glycol 3,350 28 w/v -
pH 7
560.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium Potassium phosphate pH 7 (1 M)
340.0 μl of water (100 v/v)
D10
  • Sodium Potassium phosphate 0.1 M pH 7
  • Polyethylene glycol 4,000 4 w/v -
pH 7
80.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium Potassium phosphate pH 7 (1 M)
820.0 μl of water (100 v/v)
D11
  • Sodium Potassium phosphate 0.1 M pH 7
  • Polyethylene glycol 4,000 16 w/v -
pH 7
320.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium Potassium phosphate pH 7 (1 M)
580.0 μl of water (100 v/v)
D12
  • Sodium Potassium phosphate 0.1 M pH 7
  • Polyethylene glycol 4,000 28 w/v -
pH 7
560.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium Potassium phosphate pH 7 (1 M)
340.0 μl of water (100 v/v)
E1
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 1,000 4 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
80.0 μl of Polyethylene glycol 1,000 (50 w/v)
820.0 μl of water (100 v/v)
E2
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 1,000 16 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
320.0 μl of Polyethylene glycol 1,000 (50 w/v)
580.0 μl of water (100 v/v)
E3
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 1,000 28 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
560.0 μl of Polyethylene glycol 1,000 (50 w/v)
340.0 μl of water (100 v/v)
E4
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol monomethyl ether 2,000 4 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
80.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
820.0 μl of water (100 v/v)
E5
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol monomethyl ether 2,000 16 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
320.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
580.0 μl of water (100 v/v)
E6
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol monomethyl ether 2,000 28 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
560.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
340.0 μl of water (100 v/v)
E7
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,350 4 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
80.0 μl of Polyethylene glycol 3,350 (50 w/v)
820.0 μl of water (100 v/v)
E8
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,350 16 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
320.0 μl of Polyethylene glycol 3,350 (50 w/v)
580.0 μl of water (100 v/v)
E9
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,350 28 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
560.0 μl of Polyethylene glycol 3,350 (50 w/v)
340.0 μl of water (100 v/v)
E10
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 4 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
80.0 μl of Polyethylene glycol 4,000 (50 w/v)
820.0 μl of water (100 v/v)
E11
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 16 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
320.0 μl of Polyethylene glycol 4,000 (50 w/v)
580.0 μl of water (100 v/v)
E12
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 28 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
560.0 μl of Polyethylene glycol 4,000 (50 w/v)
340.0 μl of water (100 v/v)
F1
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 1,000 4 w/v -
pH 8
80.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
820.0 μl of water (100 v/v)
F2
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 1,000 16 w/v -
pH 8
320.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
580.0 μl of water (100 v/v)
F3
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 1,000 28 w/v -
pH 8
560.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
340.0 μl of water (100 v/v)
F4
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol monomethyl ether 2,000 4 w/v -
pH 8
80.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
820.0 μl of water (100 v/v)
F5
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol monomethyl ether 2,000 16 w/v -
pH 8
320.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
580.0 μl of water (100 v/v)
F6
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol monomethyl ether 2,000 28 w/v -
pH 8
560.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
340.0 μl of water (100 v/v)
F7
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 3,350 4 w/v -
pH 8
80.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
820.0 μl of water (100 v/v)
F8
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 3,350 16 w/v -
pH 8
320.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
580.0 μl of water (100 v/v)
F9
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 3,350 28 w/v -
pH 8
560.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
340.0 μl of water (100 v/v)
F10
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 4,000 4 w/v -
pH 8
80.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
820.0 μl of water (100 v/v)
F11
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 4,000 16 w/v -
pH 8
320.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
580.0 μl of water (100 v/v)
F12
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 4,000 28 w/v -
pH 8
560.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
340.0 μl of water (100 v/v)
G1
  • BIS-TRIS propane 0.1 M pH 9
  • Polyethylene glycol 1,000 4 w/v -
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
80.0 μl of Polyethylene glycol 1,000 (50 w/v)
820.0 μl of water (100 v/v)
G2
  • BIS-TRIS propane 0.1 M pH 9
  • Polyethylene glycol 1,000 16 w/v -
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
320.0 μl of Polyethylene glycol 1,000 (50 w/v)
580.0 μl of water (100 v/v)
G3
  • BIS-TRIS propane 0.1 M pH 9
  • Polyethylene glycol 1,000 28 w/v -
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
560.0 μl of Polyethylene glycol 1,000 (50 w/v)
340.0 μl of water (100 v/v)
G4
  • BIS-TRIS propane 0.1 M pH 9
  • Polyethylene glycol monomethyl ether 2,000 4 w/v -
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
80.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
820.0 μl of water (100 v/v)
G5
  • BIS-TRIS propane 0.1 M pH 9
  • Polyethylene glycol monomethyl ether 2,000 16 w/v -
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
320.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
580.0 μl of water (100 v/v)
G6
  • BIS-TRIS propane 0.1 M pH 9
  • Polyethylene glycol monomethyl ether 2,000 28 w/v -
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
560.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
340.0 μl of water (100 v/v)
G7
  • BIS-TRIS propane 0.1 M pH 9
  • Polyethylene glycol 3,350 4 w/v -
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
80.0 μl of Polyethylene glycol 3,350 (50 w/v)
820.0 μl of water (100 v/v)
G8
  • BIS-TRIS propane 0.1 M pH 9
  • Polyethylene glycol 3,350 16 w/v -
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
320.0 μl of Polyethylene glycol 3,350 (50 w/v)
580.0 μl of water (100 v/v)
G9
  • BIS-TRIS propane 0.1 M pH 9
  • Polyethylene glycol 3,350 28 w/v -
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
560.0 μl of Polyethylene glycol 3,350 (50 w/v)
340.0 μl of water (100 v/v)
G10
  • BIS-TRIS propane 0.1 M pH 9
  • Polyethylene glycol 4,000 4 w/v -
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
80.0 μl of Polyethylene glycol 4,000 (50 w/v)
820.0 μl of water (100 v/v)
G11
  • BIS-TRIS propane 0.1 M pH 9
  • Polyethylene glycol 4,000 16 w/v -
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
320.0 μl of Polyethylene glycol 4,000 (50 w/v)
580.0 μl of water (100 v/v)
G12
  • BIS-TRIS propane 0.1 M pH 9
  • Polyethylene glycol 4,000 28 w/v -
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
560.0 μl of Polyethylene glycol 4,000 (50 w/v)
340.0 μl of water (100 v/v)
H1
  • CHES 0.1 M pH 10
  • Polyethylene glycol 1,000 4 w/v -
pH 10
100.0 μl of CHES pH 10 (1 M)
80.0 μl of Polyethylene glycol 1,000 (50 w/v)
820.0 μl of water (100 v/v)
H2
  • CHES 0.1 M pH 10
  • Polyethylene glycol 1,000 16 w/v -
pH 10
100.0 μl of CHES pH 10 (1 M)
320.0 μl of Polyethylene glycol 1,000 (50 w/v)
580.0 μl of water (100 v/v)
H3
  • CHES 0.1 M pH 10
  • Polyethylene glycol 1,000 28 w/v -
pH 10
100.0 μl of CHES pH 10 (1 M)
560.0 μl of Polyethylene glycol 1,000 (50 w/v)
340.0 μl of water (100 v/v)
H4
  • CHES 0.1 M pH 10
  • Polyethylene glycol monomethyl ether 2,000 4 w/v -
pH 10
100.0 μl of CHES pH 10 (1 M)
80.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
820.0 μl of water (100 v/v)
H5
  • CHES 0.1 M pH 10
  • Polyethylene glycol monomethyl ether 2,000 16 w/v -
pH 10
100.0 μl of CHES pH 10 (1 M)
320.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
580.0 μl of water (100 v/v)
H6
  • CHES 0.1 M pH 10
  • Polyethylene glycol monomethyl ether 2,000 28 w/v -
pH 10
100.0 μl of CHES pH 10 (1 M)
560.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
340.0 μl of water (100 v/v)
H7
  • CHES 0.1 M pH 10
  • Polyethylene glycol 3,350 4 w/v -
pH 10
100.0 μl of CHES pH 10 (1 M)
80.0 μl of Polyethylene glycol 3,350 (50 w/v)
820.0 μl of water (100 v/v)
H8
  • CHES 0.1 M pH 10
  • Polyethylene glycol 3,350 16 w/v -
pH 10
100.0 μl of CHES pH 10 (1 M)
320.0 μl of Polyethylene glycol 3,350 (50 w/v)
580.0 μl of water (100 v/v)
H9
  • CHES 0.1 M pH 10
  • Polyethylene glycol 3,350 28 w/v -
pH 10
100.0 μl of CHES pH 10 (1 M)
560.0 μl of Polyethylene glycol 3,350 (50 w/v)
340.0 μl of water (100 v/v)
H10
  • CHES 0.1 M pH 10
  • Polyethylene glycol 4,000 4 w/v -
pH 10
100.0 μl of CHES pH 10 (1 M)
80.0 μl of Polyethylene glycol 4,000 (50 w/v)
820.0 μl of water (100 v/v)
H11
  • CHES 0.1 M pH 10
  • Polyethylene glycol 4,000 16 w/v -
pH 10
100.0 μl of CHES pH 10 (1 M)
320.0 μl of Polyethylene glycol 4,000 (50 w/v)
580.0 μl of water (100 v/v)
H12
  • CHES 0.1 M pH 10
  • Polyethylene glycol 4,000 28 w/v -
pH 10
100.0 μl of CHES pH 10 (1 M)
560.0 μl of Polyethylene glycol 4,000 (50 w/v)
340.0 μl of water (100 v/v)