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Supplemental Information

Screen Description

GRAS6

GRAS screens / GRAS6
Created 2016-09-29.

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GRAS6

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GRAS6

    Screen Structure

    GRAS6

    Well Count 96
    Number of distinct ingredients 12
    Internal diversity (score mean dist) 0.895

    Screen Composition

    More information about screen composition chart.
    2025-06-14T12:59:33.183997 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type GRAS6
    Buffer 96
    Polymer 96
    Salt 36

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    GRAS6 Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 1,000 4 w/v -
    		pH 4.5
    100 μl of Sodium acetate pH 4.5 (1 M)
    80 μl of Polyethylene glycol 1,000 (50 w/v)
    820 μl of water (100 v/v)
    A2
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 1,000 16 w/v -
    		pH 4.5
    100 μl of Sodium acetate pH 4.5 (1 M)
    320 μl of Polyethylene glycol 1,000 (50 w/v)
    580 μl of water (100 v/v)
    A3
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 1,000 28 w/v -
    		pH 4.5
    100 μl of Sodium acetate pH 4.5 (1 M)
    560 μl of Polyethylene glycol 1,000 (50 w/v)
    340 μl of water (100 v/v)
    A4
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol monomethyl ether 2,000 4 w/v -
    		pH 4.5
    100 μl of Sodium acetate pH 4.5 (1 M)
    80 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    820 μl of water (100 v/v)
    A5
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol monomethyl ether 2,000 16 w/v -
    		pH 4.5
    100 μl of Sodium acetate pH 4.5 (1 M)
    320 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    580 μl of water (100 v/v)
    A6
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol monomethyl ether 2,000 28 w/v -
    		pH 4.5
    100 μl of Sodium acetate pH 4.5 (1 M)
    560 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    340 μl of water (100 v/v)
    A7
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 3,350 4 w/v -
    		pH 4.5
    100 μl of Sodium acetate pH 4.5 (1 M)
    80 μl of Polyethylene glycol 3,350 (50 w/v)
    820 μl of water (100 v/v)
    A8
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 3,350 16 w/v -
    		pH 4.5
    100 μl of Sodium acetate pH 4.5 (1 M)
    320 μl of Polyethylene glycol 3,350 (50 w/v)
    580 μl of water (100 v/v)
    A9
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 3,350 28 w/v -
    		pH 4.5
    100 μl of Sodium acetate pH 4.5 (1 M)
    560 μl of Polyethylene glycol 3,350 (50 w/v)
    340 μl of water (100 v/v)
    A10
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 4,000 4 w/v -
    		pH 4.5
    100 μl of Sodium acetate pH 4.5 (1 M)
    80 μl of Polyethylene glycol 4,000 (50 w/v)
    820 μl of water (100 v/v)
    A11
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 4,000 16 w/v -
    		pH 4.5
    100 μl of Sodium acetate pH 4.5 (1 M)
    320 μl of Polyethylene glycol 4,000 (50 w/v)
    580 μl of water (100 v/v)
    A12
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 4,000 28 w/v -
    		pH 4.5
    100 μl of Sodium acetate pH 4.5 (1 M)
    560 μl of Polyethylene glycol 4,000 (50 w/v)
    340 μl of water (100 v/v)
    B1
    • di-Sodium succinate 0.1 M pH 5.5
    • Polyethylene glycol 1,000 4 w/v -
    		pH 5.5
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    80 μl of Polyethylene glycol 1,000 (50 w/v)
    820 μl of water (100 v/v)
    B2
    • di-Sodium succinate 0.1 M pH 5.5
    • Polyethylene glycol 1,000 16 w/v -
    		pH 5.5
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    320 μl of Polyethylene glycol 1,000 (50 w/v)
    580 μl of water (100 v/v)
    B3
    • di-Sodium succinate 0.1 M pH 5.5
    • Polyethylene glycol 1,000 28 w/v -
    		pH 5.5
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    560 μl of Polyethylene glycol 1,000 (50 w/v)
    340 μl of water (100 v/v)
    B4
    • di-Sodium succinate 0.1 M pH 5.5
    • Polyethylene glycol monomethyl ether 2,000 4 w/v -
    		pH 5.5
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    80 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    820 μl of water (100 v/v)
    B5
    • di-Sodium succinate 0.1 M pH 5.5
    • Polyethylene glycol monomethyl ether 2,000 16 w/v -
    		pH 5.5
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    320 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    580 μl of water (100 v/v)
    B6
    • di-Sodium succinate 0.1 M pH 5.5
    • Polyethylene glycol monomethyl ether 2,000 28 w/v -
    		pH 5.5
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    560 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    340 μl of water (100 v/v)
    B7
    • di-Sodium succinate 0.1 M pH 5.5
    • Polyethylene glycol 3,350 4 w/v -
    		pH 5.5
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    80 μl of Polyethylene glycol 3,350 (50 w/v)
    820 μl of water (100 v/v)
    B8
    • di-Sodium succinate 0.1 M pH 5.5
    • Polyethylene glycol 3,350 16 w/v -
    		pH 5.5
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    320 μl of Polyethylene glycol 3,350 (50 w/v)
    580 μl of water (100 v/v)
    B9
    • di-Sodium succinate 0.1 M pH 5.5
    • Polyethylene glycol 3,350 28 w/v -
    		pH 5.5
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    560 μl of Polyethylene glycol 3,350 (50 w/v)
    340 μl of water (100 v/v)
    B10
    • di-Sodium succinate 0.1 M pH 5.5
    • Polyethylene glycol 4,000 4 w/v -
    		pH 5.5
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    80 μl of Polyethylene glycol 4,000 (50 w/v)
    820 μl of water (100 v/v)
    B11
    • di-Sodium succinate 0.1 M pH 5.5
    • Polyethylene glycol 4,000 16 w/v -
    		pH 5.5
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    320 μl of Polyethylene glycol 4,000 (50 w/v)
    580 μl of water (100 v/v)
    B12
    • di-Sodium succinate 0.1 M pH 5.5
    • Polyethylene glycol 4,000 28 w/v -
    		pH 5.5
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    560 μl of Polyethylene glycol 4,000 (50 w/v)
    340 μl of water (100 v/v)
    C1
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 1,000 4 w/v -
    		pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    80 μl of Polyethylene glycol 1,000 (50 w/v)
    820 μl of water (100 v/v)
    C2
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 1,000 16 w/v -
    		pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    320 μl of Polyethylene glycol 1,000 (50 w/v)
    580 μl of water (100 v/v)
    C3
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 1,000 28 w/v -
    		pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    560 μl of Polyethylene glycol 1,000 (50 w/v)
    340 μl of water (100 v/v)
    C4
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 2,000 4 w/v -
    		pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    80 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    820 μl of water (100 v/v)
    C5
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 2,000 16 w/v -
    		pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    320 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    580 μl of water (100 v/v)
    C6
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 2,000 28 w/v -
    		pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    560 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    340 μl of water (100 v/v)
    C7
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 3,350 4 w/v -
    		pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    80 μl of Polyethylene glycol 3,350 (50 w/v)
    820 μl of water (100 v/v)
    C8
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 3,350 16 w/v -
    		pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    320 μl of Polyethylene glycol 3,350 (50 w/v)
    580 μl of water (100 v/v)
    C9
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 3,350 28 w/v -
    		pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    560 μl of Polyethylene glycol 3,350 (50 w/v)
    340 μl of water (100 v/v)
    C10
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 4,000 4 w/v -
    		pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    80 μl of Polyethylene glycol 4,000 (50 w/v)
    820 μl of water (100 v/v)
    C11
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 4,000 16 w/v -
    		pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    320 μl of Polyethylene glycol 4,000 (50 w/v)
    580 μl of water (100 v/v)
    C12
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 4,000 28 w/v -
    		pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    560 μl of Polyethylene glycol 4,000 (50 w/v)
    340 μl of water (100 v/v)
    D1
    • Sodium Potassium phosphate 0.1 M pH 7
    • Polyethylene glycol 1,000 4 w/v -
    		pH 7
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    80 μl of Polyethylene glycol 1,000 (50 w/v)
    820 μl of water (100 v/v)
    D2
    • Sodium Potassium phosphate 0.1 M pH 7
    • Polyethylene glycol 1,000 16 w/v -
    		pH 7
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    320 μl of Polyethylene glycol 1,000 (50 w/v)
    580 μl of water (100 v/v)
    D3
    • Sodium Potassium phosphate 0.1 M pH 7
    • Polyethylene glycol 1,000 28 w/v -
    		pH 7
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    560 μl of Polyethylene glycol 1,000 (50 w/v)
    340 μl of water (100 v/v)
    D4
    • Sodium Potassium phosphate 0.1 M pH 7
    • Polyethylene glycol monomethyl ether 2,000 4 w/v -
    		pH 7
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    80 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    820 μl of water (100 v/v)
    D5
    • Sodium Potassium phosphate 0.1 M pH 7
    • Polyethylene glycol monomethyl ether 2,000 16 w/v -
    		pH 7
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    320 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    580 μl of water (100 v/v)
    D6
    • Sodium Potassium phosphate 0.1 M pH 7
    • Polyethylene glycol monomethyl ether 2,000 28 w/v -
    		pH 7
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    560 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    340 μl of water (100 v/v)
    D7
    • Sodium Potassium phosphate 0.1 M pH 7
    • Polyethylene glycol 3,350 4 w/v -
    		pH 7
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    80 μl of Polyethylene glycol 3,350 (50 w/v)
    820 μl of water (100 v/v)
    D8
    • Sodium Potassium phosphate 0.1 M pH 7
    • Polyethylene glycol 3,350 16 w/v -
    		pH 7
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    320 μl of Polyethylene glycol 3,350 (50 w/v)
    580 μl of water (100 v/v)
    D9
    • Sodium Potassium phosphate 0.1 M pH 7
    • Polyethylene glycol 3,350 28 w/v -
    		pH 7
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    560 μl of Polyethylene glycol 3,350 (50 w/v)
    340 μl of water (100 v/v)
    D10
    • Sodium Potassium phosphate 0.1 M pH 7
    • Polyethylene glycol 4,000 4 w/v -
    		pH 7
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    80 μl of Polyethylene glycol 4,000 (50 w/v)
    820 μl of water (100 v/v)
    D11
    • Sodium Potassium phosphate 0.1 M pH 7
    • Polyethylene glycol 4,000 16 w/v -
    		pH 7
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    320 μl of Polyethylene glycol 4,000 (50 w/v)
    580 μl of water (100 v/v)
    D12
    • Sodium Potassium phosphate 0.1 M pH 7
    • Polyethylene glycol 4,000 28 w/v -
    		pH 7
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    560 μl of Polyethylene glycol 4,000 (50 w/v)
    340 μl of water (100 v/v)
    E1
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 1,000 4 w/v -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    80 μl of Polyethylene glycol 1,000 (50 w/v)
    820 μl of water (100 v/v)
    E2
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 1,000 16 w/v -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    320 μl of Polyethylene glycol 1,000 (50 w/v)
    580 μl of water (100 v/v)
    E3
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 1,000 28 w/v -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    560 μl of Polyethylene glycol 1,000 (50 w/v)
    340 μl of water (100 v/v)
    E4
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol monomethyl ether 2,000 4 w/v -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    80 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    820 μl of water (100 v/v)
    E5
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol monomethyl ether 2,000 16 w/v -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    320 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    580 μl of water (100 v/v)
    E6
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol monomethyl ether 2,000 28 w/v -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    560 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    340 μl of water (100 v/v)
    E7
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 3,350 4 w/v -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    80 μl of Polyethylene glycol 3,350 (50 w/v)
    820 μl of water (100 v/v)
    E8
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 3,350 16 w/v -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    320 μl of Polyethylene glycol 3,350 (50 w/v)
    580 μl of water (100 v/v)
    E9
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 3,350 28 w/v -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    560 μl of Polyethylene glycol 3,350 (50 w/v)
    340 μl of water (100 v/v)
    E10
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 4 w/v -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    80 μl of Polyethylene glycol 4,000 (50 w/v)
    820 μl of water (100 v/v)
    E11
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 16 w/v -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    320 μl of Polyethylene glycol 4,000 (50 w/v)
    580 μl of water (100 v/v)
    E12
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 28 w/v -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    560 μl of Polyethylene glycol 4,000 (50 w/v)
    340 μl of water (100 v/v)
    F1
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 1,000 4 w/v -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    80 μl of Polyethylene glycol 1,000 (50 w/v)
    820 μl of water (100 v/v)
    F2
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 1,000 16 w/v -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    320 μl of Polyethylene glycol 1,000 (50 w/v)
    580 μl of water (100 v/v)
    F3
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 1,000 28 w/v -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    560 μl of Polyethylene glycol 1,000 (50 w/v)
    340 μl of water (100 v/v)
    F4
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol monomethyl ether 2,000 4 w/v -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    80 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    820 μl of water (100 v/v)
    F5
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol monomethyl ether 2,000 16 w/v -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    320 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    580 μl of water (100 v/v)
    F6
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol monomethyl ether 2,000 28 w/v -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    560 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    340 μl of water (100 v/v)
    F7
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 3,350 4 w/v -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    80 μl of Polyethylene glycol 3,350 (50 w/v)
    820 μl of water (100 v/v)
    F8
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 3,350 16 w/v -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    320 μl of Polyethylene glycol 3,350 (50 w/v)
    580 μl of water (100 v/v)
    F9
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 3,350 28 w/v -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    560 μl of Polyethylene glycol 3,350 (50 w/v)
    340 μl of water (100 v/v)
    F10
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 4,000 4 w/v -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    80 μl of Polyethylene glycol 4,000 (50 w/v)
    820 μl of water (100 v/v)
    F11
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 4,000 16 w/v -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    320 μl of Polyethylene glycol 4,000 (50 w/v)
    580 μl of water (100 v/v)
    F12
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 4,000 28 w/v -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    560 μl of Polyethylene glycol 4,000 (50 w/v)
    340 μl of water (100 v/v)
    G1
    • BIS-TRIS propane 0.1 M pH 9
    • Polyethylene glycol 1,000 4 w/v -
    		pH 9
    100 μl of BIS-TRIS propane pH 9 (1 M)
    80 μl of Polyethylene glycol 1,000 (50 w/v)
    820 μl of water (100 v/v)
    G2
    • BIS-TRIS propane 0.1 M pH 9
    • Polyethylene glycol 1,000 16 w/v -
    		pH 9
    100 μl of BIS-TRIS propane pH 9 (1 M)
    320 μl of Polyethylene glycol 1,000 (50 w/v)
    580 μl of water (100 v/v)
    G3
    • BIS-TRIS propane 0.1 M pH 9
    • Polyethylene glycol 1,000 28 w/v -
    		pH 9
    100 μl of BIS-TRIS propane pH 9 (1 M)
    560 μl of Polyethylene glycol 1,000 (50 w/v)
    340 μl of water (100 v/v)
    G4
    • BIS-TRIS propane 0.1 M pH 9
    • Polyethylene glycol monomethyl ether 2,000 4 w/v -
    		pH 9
    100 μl of BIS-TRIS propane pH 9 (1 M)
    80 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    820 μl of water (100 v/v)
    G5
    • BIS-TRIS propane 0.1 M pH 9
    • Polyethylene glycol monomethyl ether 2,000 16 w/v -
    		pH 9
    100 μl of BIS-TRIS propane pH 9 (1 M)
    320 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    580 μl of water (100 v/v)
    G6
    • BIS-TRIS propane 0.1 M pH 9
    • Polyethylene glycol monomethyl ether 2,000 28 w/v -
    		pH 9
    100 μl of BIS-TRIS propane pH 9 (1 M)
    560 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    340 μl of water (100 v/v)
    G7
    • BIS-TRIS propane 0.1 M pH 9
    • Polyethylene glycol 3,350 4 w/v -
    		pH 9
    100 μl of BIS-TRIS propane pH 9 (1 M)
    80 μl of Polyethylene glycol 3,350 (50 w/v)
    820 μl of water (100 v/v)
    G8
    • BIS-TRIS propane 0.1 M pH 9
    • Polyethylene glycol 3,350 16 w/v -
    		pH 9
    100 μl of BIS-TRIS propane pH 9 (1 M)
    320 μl of Polyethylene glycol 3,350 (50 w/v)
    580 μl of water (100 v/v)
    G9
    • BIS-TRIS propane 0.1 M pH 9
    • Polyethylene glycol 3,350 28 w/v -
    		pH 9
    100 μl of BIS-TRIS propane pH 9 (1 M)
    560 μl of Polyethylene glycol 3,350 (50 w/v)
    340 μl of water (100 v/v)
    G10
    • BIS-TRIS propane 0.1 M pH 9
    • Polyethylene glycol 4,000 4 w/v -
    		pH 9
    100 μl of BIS-TRIS propane pH 9 (1 M)
    80 μl of Polyethylene glycol 4,000 (50 w/v)
    820 μl of water (100 v/v)
    G11
    • BIS-TRIS propane 0.1 M pH 9
    • Polyethylene glycol 4,000 16 w/v -
    		pH 9
    100 μl of BIS-TRIS propane pH 9 (1 M)
    320 μl of Polyethylene glycol 4,000 (50 w/v)
    580 μl of water (100 v/v)
    G12
    • BIS-TRIS propane 0.1 M pH 9
    • Polyethylene glycol 4,000 28 w/v -
    		pH 9
    100 μl of BIS-TRIS propane pH 9 (1 M)
    560 μl of Polyethylene glycol 4,000 (50 w/v)
    340 μl of water (100 v/v)
    H1
    • CHES 0.1 M pH 10
    • Polyethylene glycol 1,000 4 w/v -
    		pH 10
    100 μl of CHES pH 10 (1 M)
    80 μl of Polyethylene glycol 1,000 (50 w/v)
    820 μl of water (100 v/v)
    H2
    • CHES 0.1 M pH 10
    • Polyethylene glycol 1,000 16 w/v -
    		pH 10
    100 μl of CHES pH 10 (1 M)
    320 μl of Polyethylene glycol 1,000 (50 w/v)
    580 μl of water (100 v/v)
    H3
    • CHES 0.1 M pH 10
    • Polyethylene glycol 1,000 28 w/v -
    		pH 10
    100 μl of CHES pH 10 (1 M)
    560 μl of Polyethylene glycol 1,000 (50 w/v)
    340 μl of water (100 v/v)
    H4
    • CHES 0.1 M pH 10
    • Polyethylene glycol monomethyl ether 2,000 4 w/v -
    		pH 10
    100 μl of CHES pH 10 (1 M)
    80 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    820 μl of water (100 v/v)
    H5
    • CHES 0.1 M pH 10
    • Polyethylene glycol monomethyl ether 2,000 16 w/v -
    		pH 10
    100 μl of CHES pH 10 (1 M)
    320 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    580 μl of water (100 v/v)
    H6
    • CHES 0.1 M pH 10
    • Polyethylene glycol monomethyl ether 2,000 28 w/v -
    		pH 10
    100 μl of CHES pH 10 (1 M)
    560 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    340 μl of water (100 v/v)
    H7
    • CHES 0.1 M pH 10
    • Polyethylene glycol 3,350 4 w/v -
    		pH 10
    100 μl of CHES pH 10 (1 M)
    80 μl of Polyethylene glycol 3,350 (50 w/v)
    820 μl of water (100 v/v)
    H8
    • CHES 0.1 M pH 10
    • Polyethylene glycol 3,350 16 w/v -
    		pH 10
    100 μl of CHES pH 10 (1 M)
    320 μl of Polyethylene glycol 3,350 (50 w/v)
    580 μl of water (100 v/v)
    H9
    • CHES 0.1 M pH 10
    • Polyethylene glycol 3,350 28 w/v -
    		pH 10
    100 μl of CHES pH 10 (1 M)
    560 μl of Polyethylene glycol 3,350 (50 w/v)
    340 μl of water (100 v/v)
    H10
    • CHES 0.1 M pH 10
    • Polyethylene glycol 4,000 4 w/v -
    		pH 10
    100 μl of CHES pH 10 (1 M)
    80 μl of Polyethylene glycol 4,000 (50 w/v)
    820 μl of water (100 v/v)
    H11
    • CHES 0.1 M pH 10
    • Polyethylene glycol 4,000 16 w/v -
    		pH 10
    100 μl of CHES pH 10 (1 M)
    320 μl of Polyethylene glycol 4,000 (50 w/v)
    580 μl of water (100 v/v)
    H12
    • CHES 0.1 M pH 10
    • Polyethylene glycol 4,000 28 w/v -
    		pH 10
    100 μl of CHES pH 10 (1 M)
    560 μl of Polyethylene glycol 4,000 (50 w/v)
    340 μl of water (100 v/v)