Jump to page contents
Welcome to the new C6 website. This website aims preserve as many features as possible of the original C6 website.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.

Supplemental Information

Screen Description

LMB

Created 2016-09-29.

Export

Barcodes

LMB

No barcodes found.

Screen Structure

LMB

Well Count 96
Number of distinct ingredients 61
Internal diversity (score mean dist) 0.954

Screen Composition

2025-08-16T06:25:38.229911 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type LMB
Buffer 110
Cryoprotectant 7
Organic (non-volatile) 19
Organic (volatile) 13
Polymer 62
Salt 118

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
LMB Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • 1,4-Butanediol 25 v/v -
  • tris chloride BCC 0.1 M pH 8
pH 8
250.0 μl of 1,4-Butanediol (100 v/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
650.0 μl of water (100 v/v)
A2
  • 2-Propanol 30 v/v -
  • Sodium cacodylate 0.1 M pH 4.6
  • tri-Sodium citrate 0.1 M -
pH 4.6
300.0 μl of 2-Propanol (100 v/v)
100.0 μl of Sodium cacodylate pH 4.6 (1 M)
62.5 μl of tri-Sodium citrate (1.6 M)
537.5 μl of water (100 v/v)
A3
  • MES 0.1 M pH 6.2
  • Calcium acetate 0.2 M -
  • 2-Propanol 9 v/v -
pH 6.2
90.0 μl of 2-Propanol (100 v/v)
100.0 μl of Calcium acetate (2 M)
57.9 μl of MES pH 6 (1 M)
42.1 μl of MES pH 6.5 (1 M)
710.0 μl of water (100 v/v)
A4
  • 2-Propanol 15 v/v -
  • Imidazole 0.1 M pH 7.6
pH 7.6
150.0 μl of 2-Propanol (100 v/v)
100.0 μl of Imidazole pH 7.6 (1 M)
750.0 μl of water (100 v/v)
A5
  • Ammonium acetate 4 M -
  • BIS-TRIS propane 0.1 M pH 7
pH 7
800.0 μl of Ammonium acetate (5 M)
100.0 μl of BIS-TRIS propane pH 7 (1 M)
100.0 μl of water (100 v/v)
A6
  • Ammonium di-hydrogen phosphate 1.4 M -
  • tris chloride BCC 0.1 M pH 7.5
pH 7.5
560.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
340.0 μl of water (100 v/v)
A7
  • Ammonium sulfate 1.85 M -
  • Sodium acetate 0.1 M pH 4.5
  • Potassium thiocyanate 0.4 M -
pH 4.5
528.6 μl of Ammonium sulfate (3.5 M)
50.0 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium acetate pH 4.5 (1 M)
321.4 μl of water (100 v/v)
A8
  • Ammonium sulfate 1.2 M -
  • Ammonium acetate 0.1 M pH 5.5
pH 5.5
100.0 μl of Ammonium acetate pH 5.5 (1 M)
342.9 μl of Ammonium sulfate (3.5 M)
557.1 μl of water (100 v/v)
A9
  • Ammonium sulfate 1 M -
  • tri-Sodium citrate 0.1 M pH 5.5
pH 5.5
285.7 μl of Ammonium sulfate (3.5 M)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
614.3 μl of water (100 v/v)
A10
  • Ammonium sulfate 2.5 M -
  • Sodium chloride 0.2 M -
  • MES 0.1 M pH 5.6
pH 5.6
714.3 μl of Ammonium sulfate (3.5 M)
100.0 μl of MES pH 5.6 (1 M)
40.0 μl of Sodium chloride (5 M)
145.7 μl of water (100 v/v)
A11
  • Ammonium sulfate 1.2 M -
  • MES 0.1 M pH 5.9
pH 5.9
342.9 μl of Ammonium sulfate (3.5 M)
27.6 μl of MES pH 5.6 (1 M)
72.4 μl of MES pH 6 (1 M)
557.1 μl of water (100 v/v)
A12
  • Ammonium sulfate 2 M -
  • MES 0.05 M pH 6
  • Magnesium acetate 0.005 M -
pH 6
571.4 μl of Ammonium sulfate (3.5 M)
50.0 μl of MES pH 6 (1 M)
5.0 μl of Magnesium acetate (1 M)
373.6 μl of water (100 v/v)
B1
  • Ammonium sulfate 1.6 M -
  • MES 0.1 M pH 6.5
pH 6.5
457.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of MES pH 6.5 (1 M)
442.9 μl of water (100 v/v)
B2
  • Ammonium sulfate 1.9 M -
  • MES 0.1 M pH 6.5
pH 6.5
542.9 μl of Ammonium sulfate (3.5 M)
100.0 μl of MES pH 6.5 (1 M)
357.1 μl of water (100 v/v)
B3
  • Ammonium sulfate 1.95 M -
  • tri-Sodium citrate 0.1 M pH 6.5
pH 6.5
557.1 μl of Ammonium sulfate (3.5 M)
62.5 μl of tri-Sodium citrate pH 6.5 (1.6 M)
380.4 μl of water (100 v/v)
B4
  • Ammonium sulfate 1.7 M -
  • tris chloride BCC 0.1 M pH 8
  • Sodium chloride 0.2 M -
pH 8
485.7 μl of Ammonium sulfate (3.5 M)
40.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
374.3 μl of water (100 v/v)
B5
  • Ammonium sulfate 1 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Glycerol 12 v/v -
pH 8.5
285.7 μl of Ammonium sulfate (3.5 M)
400.0 μl of Glycerol (30 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
214.3 μl of water (100 v/v)
B6
  • Ammonium sulfate 1 M -
  • Glycerol 6 v/v -
  • CHES 0.1 M pH 9.5
  • Sodium chloride 0.2 M -
pH 9.5
285.7 μl of Ammonium sulfate (3.5 M)
100.0 μl of CHES pH 9.5 (1 M)
200.0 μl of Glycerol (30 v/v)
40.0 μl of Sodium chloride (5 M)
374.3 μl of water (100 v/v)
B7
  • 1,4-Dioxane 38 v/v -
NA
380.0 μl of 1,4-Dioxane (100 v/v)
620.0 μl of water (100 v/v)
B8
  • Ethanol 14 v/v -
  • ADA 0.1 M pH 6
pH 6
100.0 μl of ADA pH 6 (1 M)
140.0 μl of Ethanol (100 v/v)
760.0 μl of water (100 v/v)
B9
  • Ethanol 18 v/v -
  • BIS-TRIS propane 0.1 M pH 7.2
pH 7.2
53.7 μl of BIS-TRIS propane pH 7 (1 M)
46.3 μl of BIS-TRIS propane pH 7.5 (1 M)
180.0 μl of Ethanol (100 v/v)
720.0 μl of water (100 v/v)
B10
  • Ethanol 24 v/v -
  • HEPES 0.1 M pH 7.8
  • Magnesium chloride 0.04 M -
pH 7.8
240.0 μl of Ethanol (100 v/v)
100.0 μl of HEPES pH 7.8 (1 M)
20.0 μl of Magnesium chloride (2 M)
640.0 μl of water (100 v/v)
B11
  • Ethanol 17.5 v/v -
NA
175.0 μl of Ethanol (100 v/v)
825.0 μl of water (100 v/v)
B12
  • Lithium chloride 1.3 M -
  • HEPES 0.1 M pH 7.4
pH 7.4
22.1 μl of HEPES pH 7 (1 M)
77.9 μl of HEPES pH 7.5 (1 M)
130.0 μl of Lithium chloride (10 M)
770.0 μl of water (100 v/v)
C1
  • Lithium chloride 1.5 M -
  • Polyethylene glycol 8,000 10 w/v -
  • BICINE 0.1 M pH 8.4
pH 8.4
44.1 μl of BICINE pH 7.4 (1 M)
55.9 μl of BICINE pH 9.3 (1 M)
150.0 μl of Lithium chloride (10 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
550.0 μl of water (100 v/v)
C2
  • Lithium sulfate 1.6 M -
  • tris chloride BCC 0.4 M pH 7.5
pH 7.5
533.3 μl of Lithium sulfate (3 M)
400.0 μl of tris chloride BCC pH 7.5 (1 M)
66.7 μl of water (100 v/v)
C3
  • Magnesium sulfate 1.6 M -
  • Glycerol 6 v/v -
  • HEPES 0.1 M pH 7.2
pH 7.2
60.0 μl of Glycerol (100 v/v)
64.0 μl of HEPES pH 7 (1 M)
36.0 μl of HEPES pH 7.5 (1 M)
640.0 μl of Magnesium sulfate (2.5 M)
200.0 μl of water (100 v/v)
C4
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
  • Imidazole 0.1 M pH 7
pH 7
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Imidazole pH 7 (1 M)
600.0 μl of water (100 v/v)
C5
  • 2-methyl-2,4-pentanediol BCC 70 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
700.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of water (100 v/v)
C6
  • Polyethylene glycol 1,000 18 w/v -
  • Sodium Potassium phosphate 0.1 M pH 6.2
  • Sodium chloride 0.2 M -
pH 6.2
360.0 μl of Polyethylene glycol 1,000 (50 w/v)
33.3 μl of Sodium Potassium phosphate pH 6.2 (3 M)
40.0 μl of Sodium chloride (5 M)
566.7 μl of water (100 v/v)
C7
  • Polyethylene glycol 2,000 9.5 w/v -
  • Sodium acetate 0.1 M pH 4.6
  • Magnesium chloride 1.5 mM -
pH 4.6
15.0 μl of Magnesium chloride (100 mM)
190.0 μl of Polyethylene glycol 2,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
695.0 μl of water (100 v/v)
C8
  • Polyethylene glycol 2,000 8 w/v -
  • Imidazole 0.1 M pH 7.7
  • Calcium acetate 0.3 M -
pH 7.7
300.0 μl of Calcium acetate (1 M)
68.7 μl of Imidazole pH 7.6 (1 M)
31.3 μl of Imidazole pH 8 (1 M)
160.0 μl of Polyethylene glycol 2,000 (50 w/v)
440.0 μl of water (100 v/v)
C9
  • Polyethylene glycol monomethyl ether 2,000 26 w/v -
  • BIS-TRIS 0.1 M pH 5.8
pH 5.8
81.6 μl of BIS-TRIS pH 5.5 (1 M)
18.4 μl of BIS-TRIS pH 6.5 (1 M)
520.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
380.0 μl of water (100 v/v)
C10
  • Polyethylene glycol monomethyl ether 2,000 15 w/v -
  • BIS-TRIS propane 0.1 M pH 6.9
pH 6.9
20.0 μl of BIS-TRIS propane pH 6.5 (1 M)
80.0 μl of BIS-TRIS propane pH 7 (1 M)
300.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
600.0 μl of water (100 v/v)
C11
  • Polyethylene glycol 20,000 8 w/v -
  • Polyethylene glycol monomethyl ether 550 8 v/v -
  • Sodium acetate 0.1 M pH 4.5
  • Potassium bromide 0.25 M -
pH 4.5
266.7 μl of Polyethylene glycol 20,000 (30 w/v)
80.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
62.5 μl of Potassium bromide (4 M)
100.0 μl of Sodium acetate pH 4.5 (1 M)
490.8 μl of water (100 v/v)
C12
  • Polyethylene glycol 20,000 8 w/v -
  • Polyethylene glycol monomethyl ether 550 8 v/v -
  • Sodium acetate 0.1 M pH 5.5
  • Potassium thiocyanate 0.2 M -
pH 5.5
266.7 μl of Polyethylene glycol 20,000 (30 w/v)
80.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
25.0 μl of Potassium thiocyanate (8 M)
100.0 μl of Sodium acetate pH 5.5 (1 M)
528.3 μl of water (100 v/v)
D1
  • Polyethylene glycol 20,000 7.5 w/v -
  • Polyethylene glycol monomethyl ether 550 7.5 v/v -
  • tris chloride BCC 0.1 M pH 7.5
  • Sodium formate 0.08 M -
pH 7.5
250.0 μl of Polyethylene glycol 20,000 (30 w/v)
75.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
11.4 μl of Sodium formate (7 M)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
563.6 μl of water (100 v/v)
D2
  • Polyethylene glycol 20,000 8 w/v -
  • Polyethylene glycol monomethyl ether 550 8 v/v -
  • tris chloride BCC 0.05 M pH 8.5
  • Sodium formate 1.2 M -
pH 8.5
266.7 μl of Polyethylene glycol 20,000 (30 w/v)
80.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
171.4 μl of Sodium formate (7 M)
50.0 μl of tris chloride BCC pH 8.5 (1 M)
431.9 μl of water (100 v/v)
D3
  • Polyethylene glycol 300 28 v/v -
  • Imidazole 0.1 M pH 7
  • Calcium acetate 0.07 M -
pH 7
70.0 μl of Calcium acetate (1 M)
100.0 μl of Imidazole pH 7 (1 M)
280.0 μl of Polyethylene glycol 300 (100 v/v)
550.0 μl of water (100 v/v)
D4
  • Polyethylene glycol 300 30 v/v -
  • Polyethylene glycol 1,000 15 w/v -
  • BIS-TRIS propane 0.1 M pH 7
pH 7
100.0 μl of BIS-TRIS propane pH 7 (1 M)
300.0 μl of Polyethylene glycol 1,000 (50 w/v)
300.0 μl of Polyethylene glycol 300 (100 v/v)
300.0 μl of water (100 v/v)
D5
  • Polyethylene glycol 3,000 26 w/v -
  • CHES 0.1 M pH 9.2
pH 9.2
64.0 μl of CHES pH 9 (1 M)
36.0 μl of CHES pH 9.5 (1 M)
520.0 μl of Polyethylene glycol 3,000 (50 w/v)
380.0 μl of water (100 v/v)
D6
  • Polyethylene glycol 3,350 21 w/v -
  • MES 0.1 M pH 6
  • Sodium chloride 0.15 M -
pH 6
100.0 μl of MES pH 6 (1 M)
420.0 μl of Polyethylene glycol 3,350 (50 w/v)
30.0 μl of Sodium chloride (5 M)
450.0 μl of water (100 v/v)
D7
  • Polyethylene glycol 3,350 15 w/v -
  • MES 0.1 M pH 6.2
pH 6.2
57.9 μl of MES pH 6 (1 M)
42.1 μl of MES pH 6.5 (1 M)
300.0 μl of Polyethylene glycol 3,350 (50 w/v)
600.0 μl of water (100 v/v)
D8
  • Polyethylene glycol 3,350 24 w/v -
  • Polyethylene glycol 400 0.2 v/v -
  • HEPES 0.05 M pH 6.8
  • Sodium chloride 0.15 M -
pH 6.8
28.8 μl of HEPES pH 6.6 (1 M)
21.2 μl of HEPES pH 7 (1 M)
480.0 μl of Polyethylene glycol 3,350 (50 w/v)
2.0 μl of Polyethylene glycol 400 (100 v/v)
30.0 μl of Sodium chloride (5 M)
438.0 μl of water (100 v/v)
D9
  • Polyethylene glycol 3,350 27 w/v -
  • BIS-TRIS propane 0.1 M pH 7
  • Lithium sulfate 0.2 M -
pH 7
100.0 μl of BIS-TRIS propane pH 7 (1 M)
100.0 μl of Lithium sulfate (2 M)
540.0 μl of Polyethylene glycol 3,350 (50 w/v)
260.0 μl of water (100 v/v)
D10
  • Polyethylene glycol 3,350 12 w/v -
  • HEPES 0.1 M pH 7.5
  • Cobalt (II) chloride 5 mM -
  • Magnesium chloride 5 mM -
  • Nickel (II) chloride 5 mM -
pH 7.5
50.0 μl of Cobalt (II) chloride (100 mM)
100.0 μl of HEPES pH 7.5 (1 M)
50.0 μl of Magnesium chloride (100 mM)
50.0 μl of Nickel (II) chloride (100 mM)
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
510.0 μl of water (100 v/v)
D11
  • Polyethylene glycol 3,350 20 w/v -
  • tris chloride BCC 0.2 M pH 8.5
pH 8.5
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
D12
  • Polyethylene glycol 3,350 18 w/v -
  • 2-Propanol 4.8 v/v -
  • CAPSO 0.1 M pH 9
pH 9
48.0 μl of 2-Propanol (100 v/v)
189.7 μl of CAPSO pH 8.9 (0.5 M)
10.3 μl of CAPSO pH 10 (0.5 M)
360.0 μl of Polyethylene glycol 3,350 (50 w/v)
392.0 μl of water (100 v/v)
E1
  • Polyethylene glycol 3,350 6 w/v -
  • Sodium chloride 0.15 M -
  • Potassium iodide 0.4 M -
NA
120.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of Potassium iodide (1 M)
30.0 μl of Sodium chloride (5 M)
450.0 μl of water (100 v/v)
E2
  • Polyethylene glycol 3,350 18 w/v -
  • Sodium acetate 0.2 M -
NA
360.0 μl of Polyethylene glycol 3,350 (50 w/v)
66.7 μl of Sodium acetate (3 M)
573.3 μl of water (100 v/v)
E3
  • Polyethylene glycol 3,350 22 w/v -
  • Zinc acetate 0.2 M -
NA
440.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of Zinc acetate (1 M)
360.0 μl of water (100 v/v)
E4
  • Polyethylene glycol 400 26 v/v -
  • tri-Sodium citrate 0.1 M pH 5.5
  • Magnesium chloride 0.1 M -
  • Sodium chloride 0.1 M -
pH 5.5
50.0 μl of Magnesium chloride (2 M)
260.0 μl of Polyethylene glycol 400 (100 v/v)
20.0 μl of Sodium chloride (5 M)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
570.0 μl of water (100 v/v)
E5
  • Polyethylene glycol 400 4 v/v -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Lithium sulfate 0.1 M -
pH 5.6
50.0 μl of Lithium sulfate (2 M)
40.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
810.0 μl of water (100 v/v)
E6
  • Polyethylene glycol 400 10 v/v -
  • MES 0.05 M pH 6
  • Potassium chloride 0.1 M -
  • Magnesium chloride 2 mM -
pH 6
50.0 μl of MES pH 6 (1 M)
20.0 μl of Magnesium chloride (100 mM)
100.0 μl of Polyethylene glycol 400 (100 v/v)
25.0 μl of Potassium chloride (4 M)
805.0 μl of water (100 v/v)
E7
  • Polyethylene glycol 400 28 v/v -
  • MOPS 0.1 M pH 6.5
  • Sodium chloride 0.2 M -
pH 6.5
100.0 μl of MOPS pH 6.5 (1 M)
280.0 μl of Polyethylene glycol 400 (100 v/v)
40.0 μl of Sodium chloride (5 M)
580.0 μl of water (100 v/v)
E8
  • Polyethylene glycol 400 25 v/v -
  • Ethanol 4.5 v/v -
  • MES 0.07 M pH 6.6
  • Magnesium chloride 1.5 mM -
pH 6.6
45.0 μl of Ethanol (100 v/v)
70.0 μl of MES pH 6.6 (1 M)
15.0 μl of Magnesium chloride (100 mM)
250.0 μl of Polyethylene glycol 400 (100 v/v)
620.0 μl of water (100 v/v)
E9
  • Polyethylene glycol 400 40 v/v -
  • tris chloride BCC 0.1 M pH 8.4
  • Lithium sulfate 0.2 M -
pH 8.4
100.0 μl of Lithium sulfate (2 M)
400.0 μl of Polyethylene glycol 400 (100 v/v)
18.0 μl of tris chloride BCC pH 8 (1 M)
82.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
E10
  • Polyethylene glycol 4,000 3.5 w/v -
  • Glycerol 15 v/v -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
500.0 μl of Glycerol (30 v/v)
70.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
330.0 μl of water (100 v/v)
E11
  • Polyethylene glycol 4,000 28 w/v -
  • tri-Sodium citrate 0.1 M pH 5.2
  • Ammonium acetate 0.2 M -
pH 5.2
200.0 μl of Ammonium acetate (1 M)
560.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.2 (1 M)
140.0 μl of water (100 v/v)
E12
  • Polyethylene glycol 4,000 20 w/v -
  • 2-Propanol 20 v/v -
  • tri-Sodium citrate 0.1 M pH 5.5
pH 5.5
200.0 μl of 2-Propanol (100 v/v)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
300.0 μl of water (100 v/v)
F1
  • Polyethylene glycol 4,000 25 w/v -
  • Sodium acetate 0.1 M pH 5.6
  • Ammonium sulfate 0.2 M -
pH 5.6
57.1 μl of Ammonium sulfate (3.5 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
33.7 μl of Sodium acetate pH 5 (1 M)
66.3 μl of Sodium acetate pH 6.8 (1 M)
342.9 μl of water (100 v/v)
F2
  • Polyethylene glycol 4,000 16 w/v -
  • Glycerol 20 v/v -
  • tri-Sodium citrate 0.1 M pH 5.8
  • Ammonium sulfate 0.1 M -
pH 5.8
28.6 μl of Ammonium sulfate (3.5 M)
200.0 μl of Glycerol (100 v/v)
320.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.8 (1 M)
351.4 μl of water (100 v/v)
F3
  • Polyethylene glycol 4,000 18 w/v -
  • tri-Sodium citrate 0.1 M pH 6
  • Ammonium acetate 0.2 M -
pH 6
200.0 μl of Ammonium acetate (1 M)
360.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 6 (1 M)
340.0 μl of water (100 v/v)
F4
  • Polyethylene glycol 4,000 14 w/v -
  • 2-methyl-2,4-pentanediol BCC 6 v/v -
  • Sodium Potassium phosphate 0.1 M pH 6.2
pH 6.2
60.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
280.0 μl of Polyethylene glycol 4,000 (50 w/v)
33.3 μl of Sodium Potassium phosphate pH 6.2 (3 M)
626.7 μl of water (100 v/v)
F5
  • Polyethylene glycol 4,000 29 w/v -
  • tri-Sodium citrate 0.1 M pH 6.5
  • Magnesium acetate 0.1 M -
  • Ammonium sulfate 0.1 M -
pH 6.5
28.6 μl of Ammonium sulfate (3.5 M)
100.0 μl of Magnesium acetate (1 M)
580.0 μl of Polyethylene glycol 4,000 (50 w/v)
62.5 μl of tri-Sodium citrate pH 6.5 (1.6 M)
228.9 μl of water (100 v/v)
F6
  • Polyethylene glycol 4,000 11 w/v -
  • tris chloride BCC 0.1 M pH 7.8
  • Magnesium chloride 0.1 M -
pH 7.8
50.0 μl of Magnesium chloride (2 M)
220.0 μl of Polyethylene glycol 4,000 (50 w/v)
45.0 μl of tris chloride BCC pH 7.5 (1 M)
55.0 μl of tris chloride BCC pH 8 (1 M)
630.0 μl of water (100 v/v)
F7
  • Polyethylene glycol 4,000 20 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Magnesium chloride 0.2 M -
pH 8.5
100.0 μl of Magnesium chloride (2 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
F8
  • Polyethylene glycol 4,000 18 w/v -
  • tris chloride BCC 0.1 M pH 9
  • Sodium acetate 0.3 M -
pH 9
360.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate (3 M)
100.0 μl of tris chloride BCC pH 9 (1 M)
440.0 μl of water (100 v/v)
F9
  • Polyethylene glycol monomethyl ether 5,000 18 w/v -
  • MES 0.1 M pH 6.5
  • Ammonium sulfate 0.2 M -
pH 6.5
57.1 μl of Ammonium sulfate (3.5 M)
200.0 μl of MES pH 6.5 (0.5 M)
360.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
382.9 μl of water (100 v/v)
F10
  • Polyethylene glycol 600 20 v/v -
  • Sodium cacodylate 0.1 M pH 5.6
  • Potassium thiocyanate 0.15 M -
  • Sodium chloride 0.2 M -
pH 5.6
200.0 μl of Polyethylene glycol 600 (100 v/v)
18.8 μl of Potassium thiocyanate (8 M)
84.4 μl of Sodium cacodylate pH 5.5 (1 M)
15.6 μl of Sodium cacodylate pH 6 (1 M)
40.0 μl of Sodium chloride (5 M)
641.2 μl of water (100 v/v)
F11
  • Polyethylene glycol 6,000 29 w/v -
  • tri-Sodium citrate 0.2 M pH 3.5
pH 3.5
580.0 μl of Polyethylene glycol 6,000 (50 w/v)
200.0 μl of tri-Sodium citrate pH 3.5 (1 M)
220.0 μl of water (100 v/v)
F12
  • Polyethylene glycol 6,000 20 w/v -
  • tri-Sodium citrate 0.1 M pH 4
  • Lithium chloride 0.2 M -
pH 4
20.0 μl of Lithium chloride (10 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
63.0 μl of tri-Sodium citrate pH 3.5 (1 M)
37.0 μl of tri-Sodium citrate pH 5.2 (1 M)
480.0 μl of water (100 v/v)
G1
  • Polyethylene glycol 6,000 12 w/v -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Lithium sulfate 0.1 M -
pH 5.6
50.0 μl of Lithium sulfate (2 M)
240.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
610.0 μl of water (100 v/v)
G2
  • Polyethylene glycol 6,000 3.5 w/v -
  • BIS-TRIS propane 0.1 M pH 7.1
  • Potassium chloride 0.1 M -
pH 7.1
76.0 μl of BIS-TRIS propane pH 7 (1 M)
24.0 μl of BIS-TRIS propane pH 7.5 (1 M)
70.0 μl of Polyethylene glycol 6,000 (50 w/v)
25.0 μl of Potassium chloride (4 M)
805.0 μl of water (100 v/v)
G3
  • Polyethylene glycol 8,000 8 w/v -
  • Sodium acetate 0.1 M pH 4.5
  • Magnesium chloride 50 mM -
pH 4.5
500.0 μl of Magnesium chloride (100 mM)
160.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
240.0 μl of water (100 v/v)
G4
  • Polyethylene glycol 8,000 22 w/v -
  • Sodium cacodylate 0.1 M pH 5
  • Sodium acetate 0.2 M -
pH 5
440.0 μl of Polyethylene glycol 8,000 (50 w/v)
66.7 μl of Sodium acetate (3 M)
75.4 μl of Sodium cacodylate pH 4.6 (1 M)
24.6 μl of Sodium cacodylate pH 5.5 (1 M)
393.3 μl of water (100 v/v)
G5
  • Polyethylene glycol 8,000 8 w/v -
  • Potassium di-hydrogen phosphate 0.08 M pH 5.6
pH 5.6
160.0 μl of Polyethylene glycol 8,000 (50 w/v)
80.0 μl of Potassium di-hydrogen phosphate pH 5.6 (1 M)
760.0 μl of water (100 v/v)
G6
  • Polyethylene glycol 8,000 11 w/v -
  • MES 0.1 M pH 6
  • Ammonium sulfate 0.24 M -
pH 6
68.6 μl of Ammonium sulfate (3.5 M)
100.0 μl of MES pH 6 (1 M)
220.0 μl of Polyethylene glycol 8,000 (50 w/v)
611.4 μl of water (100 v/v)
G7
  • Polyethylene glycol 8,000 11 w/v -
  • Sodium cacodylate 0.1 M pH 6.2
  • Sodium chloride 0.4 M -
pH 6.2
220.0 μl of Polyethylene glycol 8,000 (50 w/v)
59.5 μl of Sodium cacodylate pH 6 (1 M)
40.5 μl of Sodium cacodylate pH 6.5 (1 M)
80.0 μl of Sodium chloride (5 M)
600.0 μl of water (100 v/v)
G8
  • Polyethylene glycol 8,000 13 w/v -
  • Sodium cacodylate 0.1 M pH 6.5
  • Ammonium sulfate 0.09 M -
pH 6.5
25.7 μl of Ammonium sulfate (3.5 M)
260.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
614.3 μl of water (100 v/v)
G9
  • Polyethylene glycol 8,000 20 w/v -
  • MES 0.1 M pH 6.5
  • Magnesium acetate 0.2 M -
pH 6.5
200.0 μl of MES pH 6.5 (0.5 M)
200.0 μl of Magnesium acetate (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
200.0 μl of water (100 v/v)
G10
  • Polyethylene glycol 8,000 10 w/v -
  • Ethylene glycol 9 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
90.0 μl of Ethylene glycol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
610.0 μl of water (100 v/v)
G11
  • Polyethylene glycol 8,000 20 w/v -
  • CAPS 0.1 M pH 9
  • Magnesium chloride 0.2 M -
pH 9
100.0 μl of CAPS pH 9 (1 M)
100.0 μl of Magnesium chloride (2 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
400.0 μl of water (100 v/v)
G12
  • Polyethylene glycol 8,000 20 w/v -
  • CHES 0.1 M pH 9.5
pH 9.5
100.0 μl of CHES pH 9.5 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
500.0 μl of water (100 v/v)
H1
  • Potassium di-hydrogen phosphate 1 M -
  • 2-Propanol 3 v/v -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
30.0 μl of 2-Propanol (100 v/v)
500.0 μl of Potassium di-hydrogen phosphate (2 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
370.0 μl of water (100 v/v)
H2
  • Sodium acetate 1.4 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
466.7 μl of Sodium acetate (3 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
433.3 μl of water (100 v/v)
H3
  • Sodium bromide 0.5 M -
  • tris chloride BCC 0.1 M pH 7.5
pH 7.5
100.0 μl of Sodium bromide (5 M)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
800.0 μl of water (100 v/v)
H4
  • Sodium chloride 2 M -
  • tri-Sodium citrate 0.1 M pH 4
pH 4
400.0 μl of Sodium chloride (5 M)
63.0 μl of tri-Sodium citrate pH 3.5 (1 M)
37.0 μl of tri-Sodium citrate pH 5.2 (1 M)
500.0 μl of water (100 v/v)
H5
  • Sodium chloride 3 M -
  • tris chloride BCC 0.1 M pH 7.5
pH 7.5
600.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
300.0 μl of water (100 v/v)
H6
  • tri-Sodium citrate 1.6 M pH 6.5
pH 6.5
1000.0 μl of tri-Sodium citrate pH 6.5 (1.6 M)
H7
  • tri-Sodium citrate 0.85 M -
  • tris chloride BCC 0.1 M pH 8
  • Sodium chloride 0.1 M -
pH 8
20.0 μl of Sodium chloride (5 M)
531.2 μl of tri-Sodium citrate (1.6 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
348.8 μl of water (100 v/v)
H8
  • Sodium formate 1.36 M -
  • Polyethylene glycol 35,000 3 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Glycerol 19 v/v -
pH 8.5
190.0 μl of Glycerol (100 v/v)
150.0 μl of Polyethylene glycol 35,000 (20 w/v)
194.3 μl of Sodium formate (7 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
365.7 μl of water (100 v/v)
H9
  • Potassium Sodium tartrate 0.45 M -
  • MES 0.1 M pH 6.3
pH 6.3
37.5 μl of MES pH 6 (1 M)
62.5 μl of MES pH 6.5 (1 M)
300.0 μl of Potassium Sodium tartrate (1.5 M)
600.0 μl of water (100 v/v)
H10
  • Potassium Sodium tartrate 0.9 M -
  • HEPES 0.05 M pH 7.4
  • Glycerol 20 v/v -
pH 7.4
200.0 μl of Glycerol (100 v/v)
11.0 μl of HEPES pH 7 (1 M)
39.0 μl of HEPES pH 7.5 (1 M)
600.0 μl of Potassium Sodium tartrate (1.5 M)
150.0 μl of water (100 v/v)
H11
  • Sodium Potassium phosphate 1.6 M pH 6
pH 6
533.3 μl of Sodium Potassium phosphate pH 6 (3 M)
466.7 μl of water (100 v/v)
H12
  • tri-Sodium citrate 1 M pH 6.2
pH 6.2
1000.0 μl of tri-Sodium citrate pH 6.2 (1 M)