C6 - Screen Contents - Crystal Screen 1

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Supplemental Information

Screen Description

Crystal Screen 1

Hampton Research / Crystal Screen 1

Created 2009-11-16.

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Crystal Screen 1

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Crystal Screen 1

Screen Structure

Crystal Screen 1

Well Count	96
Number of distinct ingredients	28
Internal diversity (score mean dist)	0.938

Screen Composition

More information about screen composition chart.

ingredient type	Crystal Screen 1
Buffer	62
Organic (non-volatile)	8
Organic (volatile)	7
Polymer	25
Salt	65

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Crystal Screen 1 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Calcium chloride 0.02 M - Sodium acetate 0.1 M pH 4.6 2-methyl-2,4-pentanediol BCC 30 v/v - 🔍	pH 4.6	300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 10 μl of Calcium chloride (2 M) 100 μl of Sodium acetate pH 4.6 (1 M) 590 μl of water (100 v/v)
A2	Potassium Sodium tartrate 0.4 M - 🔍	NA	266 μl of Potassium Sodium tartrate (1.5 M) 733 μl of water (100 v/v)
A3	Ammonium di-hydrogen phosphate 0.4 M - 🔍	NA	160 μl of Ammonium di-hydrogen phosphate (2.5 M) 840 μl of water (100 v/v)
A4	tris chloride BCC 0.1 M pH 8.5 Ammonium sulfate 2 M - 🔍	pH 8.5	571 μl of Ammonium sulfate (3.5 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 328 μl of water (100 v/v)
A5	tri-Sodium citrate 0.2 M - HEPES 0.1 M pH 7.5 2-methyl-2,4-pentanediol BCC 30 v/v - 🔍	pH 7.5	300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 125 μl of tri-Sodium citrate (1.6 M) 100 μl of HEPES pH 7.5 (1 M) 475 μl of water (100 v/v)
A6	Magnesium chloride 0.2 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 4,000 30 w/v - 🔍	pH 8.5	600 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 100 μl of Magnesium chloride (2 M) 200 μl of water (100 v/v)
A7	Sodium cacodylate 0.1 M pH 6.5 Sodium acetate 1.4 M - 🔍	pH 6.5	100 μl of Sodium cacodylate pH 6.5 (1 M) 466 μl of Sodium acetate (3 M) 433 μl of water (100 v/v)
A8	tri-Sodium citrate 0.2 M - Sodium cacodylate 0.1 M pH 6.5 2-Propanol 30 v/v - 🔍	pH 6.5	100 μl of Sodium cacodylate pH 6.5 (1 M) 125 μl of tri-Sodium citrate (1.6 M) 300 μl of 2-Propanol (100 v/v) 475 μl of water (100 v/v)
A9	Ammonium acetate 0.2 M - tri-Sodium citrate 0.1 M pH 5.6 Polyethylene glycol 4,000 30 w/v - 🔍	pH 5.6	200 μl of Ammonium acetate (1 M) 600 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of tri-Sodium citrate pH 5.6 (1 M) 100 μl of water (100 v/v)
A10	Ammonium acetate 0.2 M - Sodium acetate 0.1 M pH 4.6 Polyethylene glycol 4,000 30 w/v - 🔍	pH 4.6	200 μl of Ammonium acetate (1 M) 100 μl of Sodium acetate pH 4.6 (1 M) 600 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of water (100 v/v)
A11	tri-Sodium citrate 0.1 M pH 5.6 Ammonium di-hydrogen phosphate 1 M - 🔍	pH 5.6	400 μl of Ammonium di-hydrogen phosphate (2.5 M) 100 μl of tri-Sodium citrate pH 5.6 (1 M) 500 μl of water (100 v/v)
A12	Magnesium chloride 0.2 M - HEPES 0.1 M pH 7.5 2-Propanol 30 v/v - 🔍	pH 7.5	100 μl of Magnesium chloride (2 M) 300 μl of 2-Propanol (100 v/v) 100 μl of HEPES pH 7.5 (1 M) 500 μl of water (100 v/v)
B1	tri-Sodium citrate 0.2 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 400 30 v/v - 🔍	pH 8.5	300 μl of Polyethylene glycol 400 (100 v/v) 125 μl of tri-Sodium citrate (1.6 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 475 μl of water (100 v/v)
B2	Calcium chloride 0.2 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 400 28 v/v - 🔍	pH 7.5	100 μl of Calcium chloride (2 M) 280 μl of Polyethylene glycol 400 (100 v/v) 100 μl of HEPES pH 7.5 (1 M) 520 μl of water (100 v/v)
B3	Ammonium sulfate 0.2 M - Sodium cacodylate 0.1 M pH 6.5 Polyethylene glycol 8,000 30 w/v - 🔍	pH 6.5	600 μl of Polyethylene glycol 8,000 (50 w/v) 57 μl of Ammonium sulfate (3.5 M) 100 μl of Sodium cacodylate pH 6.5 (1 M) 242 μl of water (100 v/v)
B4	HEPES 0.1 M pH 7.5 Lithium sulfate 1.5 M - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 750 μl of Lithium sulfate (2 M) 150 μl of water (100 v/v)
B5	Lithium sulfate 0.2 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 4,000 30 w/v - 🔍	pH 8.5	600 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 100 μl of Lithium sulfate (2 M) 200 μl of water (100 v/v)
B6	Magnesium acetate 0.2 M - Sodium cacodylate 0.1 M pH 6.5 Polyethylene glycol 8,000 20 w/v - 🔍	pH 6.5	400 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of Sodium cacodylate pH 6.5 (1 M) 200 μl of Magnesium acetate (1 M) 300 μl of water (100 v/v)
B7	Ammonium acetate 0.2 M - tris chloride BCC 0.1 M pH 8.5 2-Propanol 30 v/v - 🔍	pH 8.5	200 μl of Ammonium acetate (1 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 300 μl of 2-Propanol (100 v/v) 400 μl of water (100 v/v)
B8	Ammonium sulfate 0.2 M - Sodium acetate 0.1 M pH 4.6 Polyethylene glycol 4,000 25 w/v - 🔍	pH 4.6	57 μl of Ammonium sulfate (3.5 M) 100 μl of Sodium acetate pH 4.6 (1 M) 500 μl of Polyethylene glycol 4,000 (50 w/v) 342 μl of water (100 v/v)
B9	Magnesium acetate 0.2 M - Sodium cacodylate 0.1 M pH 6.5 2-methyl-2,4-pentanediol BCC 30 v/v - 🔍	pH 6.5	300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100 μl of Sodium cacodylate pH 6.5 (1 M) 200 μl of Magnesium acetate (1 M) 400 μl of water (100 v/v)
B10	Sodium acetate 0.2 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 4,000 30 w/v - 🔍	pH 8.5	66 μl of Sodium acetate (3 M) 600 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 233 μl of water (100 v/v)
B11	Magnesium chloride 0.2 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 400 30 v/v - 🔍	pH 7.5	300 μl of Polyethylene glycol 400 (100 v/v) 100 μl of Magnesium chloride (2 M) 100 μl of HEPES pH 7.5 (1 M) 500 μl of water (100 v/v)
B12	Calcium chloride 0.2 M - Sodium acetate 0.1 M pH 4.6 2-Propanol 20 v/v - 🔍	pH 4.6	100 μl of Calcium chloride (2 M) 100 μl of Sodium acetate pH 4.6 (1 M) 200 μl of 2-Propanol (100 v/v) 600 μl of water (100 v/v)
C1	Imidazole 0.1 M pH 6.5 Sodium acetate 1 M - 🔍	pH 6.5	333 μl of Sodium acetate (3 M) 100 μl of Imidazole pH 6.5 (1 M) 566 μl of water (100 v/v)
C2	Ammonium acetate 0.2 M - tri-Sodium citrate 0.1 M pH 5.6 2-methyl-2,4-pentanediol BCC 30 v/v - 🔍	pH 5.6	300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 200 μl of Ammonium acetate (1 M) 100 μl of tri-Sodium citrate pH 5.6 (1 M) 400 μl of water (100 v/v)
C3	tri-Sodium citrate 0.2 M - HEPES 0.1 M pH 7.5 2-Propanol 20 v/v - 🔍	pH 7.5	125 μl of tri-Sodium citrate (1.6 M) 200 μl of 2-Propanol (100 v/v) 100 μl of HEPES pH 7.5 (1 M) 575 μl of water (100 v/v)
C4	Sodium acetate 0.2 M - Sodium cacodylate 0.1 M pH 6.5 Polyethylene glycol 8,000 30 w/v - 🔍	pH 6.5	600 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of Sodium cacodylate pH 6.5 (1 M) 66 μl of Sodium acetate (3 M) 233 μl of water (100 v/v)
C5	HEPES 0.1 M pH 7.5 Potassium Sodium tartrate 0.8 M - 🔍	pH 7.5	533 μl of Potassium Sodium tartrate (1.5 M) 100 μl of HEPES pH 7.5 (1 M) 366 μl of water (100 v/v)
C6	Ammonium sulfate 0.2 M - Polyethylene glycol 8,000 30 w/v - 🔍	NA	600 μl of Polyethylene glycol 8,000 (50 w/v) 57 μl of Ammonium sulfate (3.5 M) 342 μl of water (100 v/v)
C7	Ammonium sulfate 0.2 M - Polyethylene glycol 4,000 30 w/v - 🔍	NA	57 μl of Ammonium sulfate (3.5 M) 600 μl of Polyethylene glycol 4,000 (50 w/v) 342 μl of water (100 v/v)
C8	Ammonium sulfate 2 M - 🔍	NA	571 μl of Ammonium sulfate (3.5 M) 428 μl of water (100 v/v)
C9	Sodium formate 4 M - 🔍	NA	571 μl of Sodium formate (7 M) 428 μl of water (100 v/v)
C10	Sodium formate 2 M - Sodium acetate 0.1 M pH 4.6 🔍	pH 4.6	100 μl of Sodium acetate pH 4.6 (1 M) 285 μl of Sodium formate (7 M) 614 μl of water (100 v/v)
C11	HEPES 0.1 M pH 7.5 Sodium di-hydrogen phosphate 0.8 M - Potassium di-hydrogen phosphate 0.8 M - 🔍	pH 7.5	200 μl of Sodium di-hydrogen phosphate (4 M) 533 μl of Potassium di-hydrogen phosphate (1.5 M) 100 μl of HEPES pH 7.5 (1 M) 166 μl of water (100 v/v)
C12	tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 8,000 8 w/v - 🔍	pH 8.5	160 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 740 μl of water (100 v/v)
D1	Sodium acetate 0.1 M pH 4.6 Polyethylene glycol 4,000 8 w/v - 🔍	pH 4.6	100 μl of Sodium acetate pH 4.6 (1 M) 160 μl of Polyethylene glycol 4,000 (50 w/v) 740 μl of water (100 v/v)
D2	HEPES 0.1 M pH 7.5 tri-Sodium citrate 1.4 M - 🔍	pH 7.5	875 μl of tri-Sodium citrate (1.6 M) 100 μl of HEPES pH 7.5 (1 M) 25 μl of water (100 v/v)
D3	HEPES 0.1 M pH 7.5 Polyethylene glycol 400 2 v/v - Ammonium sulfate 2 M - 🔍	pH 7.5	571 μl of Ammonium sulfate (3.5 M) 20 μl of Polyethylene glycol 400 (100 v/v) 100 μl of HEPES pH 7.5 (1 M) 308 μl of water (100 v/v)
D4	tri-Sodium citrate 0.1 M pH 5.6 2-Propanol 20 v/v - Polyethylene glycol 4,000 20 w/v - 🔍	pH 5.6	400 μl of Polyethylene glycol 4,000 (50 w/v) 200 μl of 2-Propanol (100 v/v) 100 μl of tri-Sodium citrate pH 5.6 (1 M) 300 μl of water (100 v/v)
D5	HEPES 0.1 M pH 7.5 2-Propanol 10 v/v - Polyethylene glycol 4,000 20 w/v - 🔍	pH 7.5	400 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of 2-Propanol (100 v/v) 100 μl of HEPES pH 7.5 (1 M) 400 μl of water (100 v/v)
D6	Potassium di-hydrogen phosphate 0.05 M - Polyethylene glycol 8,000 20 w/v - 🔍	NA	400 μl of Polyethylene glycol 8,000 (50 w/v) 33 μl of Potassium di-hydrogen phosphate (1.5 M) 566 μl of water (100 v/v)
D7	Polyethylene glycol 1,500 30 w/v - 🔍	NA	600 μl of Polyethylene glycol 1,500 (50 w/v) 400 μl of water (100 v/v)
D8	Magnesium formate 0.2 M - 🔍	NA	200 μl of Magnesium formate (1 M) 800 μl of water (100 v/v)
D9	Zinc acetate 0.2 M - Sodium cacodylate 0.1 M pH 6.5 Polyethylene glycol 8,000 18 w/v - 🔍	pH 6.5	360 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of Sodium cacodylate pH 6.5 (1 M) 200 μl of Zinc acetate (1 M) 340 μl of water (100 v/v)
D10	Calcium acetate 0.2 M - Sodium cacodylate 0.1 M pH 6.5 Polyethylene glycol 8,000 18 w/v - 🔍	pH 6.5	360 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of Sodium cacodylate pH 6.5 (1 M) 200 μl of Calcium acetate (1 M) 340 μl of water (100 v/v)
D11	Ammonium sulfate 2 M - Sodium acetate 0.1 M pH 4.6 🔍	pH 4.6	571 μl of Ammonium sulfate (3.5 M) 100 μl of Sodium acetate pH 4.6 (1 M) 328 μl of water (100 v/v)
D12	tris chloride BCC 0.1 M pH 8.5 Ammonium di-hydrogen phosphate 2 M - 🔍	pH 8.5	800 μl of Ammonium di-hydrogen phosphate (2.5 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 100 μl of water (100 v/v)
E1	Lithium sulfate 1 M - Polyethylene glycol 8,000 2 w/v - 🔍	NA	40 μl of Polyethylene glycol 8,000 (50 w/v) 500 μl of Lithium sulfate (2 M) 460 μl of water (100 v/v)
E2	Lithium sulfate 0.5 M - Polyethylene glycol 8,000 15 w/v - 🔍	NA	300 μl of Polyethylene glycol 8,000 (50 w/v) 250 μl of Lithium sulfate (2 M) 450 μl of water (100 v/v)
E3	🔍	NA	check warnings
E4	🔍	NA	check warnings
E5	🔍	NA	check warnings
E6	🔍	NA	check warnings
E7	🔍	NA	check warnings
E8	🔍	NA	check warnings
E9	🔍	NA	check warnings
E10	🔍	NA	check warnings
E11	🔍	NA	check warnings
E12	🔍	NA	check warnings
F1	🔍	NA	check warnings
F2	🔍	NA	check warnings
F3	🔍	NA	check warnings
F4	🔍	NA	check warnings
F5	🔍	NA	check warnings
F6	🔍	NA	check warnings
F7	🔍	NA	check warnings
F8	🔍	NA	check warnings
F9	🔍	NA	check warnings
F10	🔍	NA	check warnings
F11	🔍	NA	check warnings
F12	🔍	NA	check warnings
G1	🔍	NA	check warnings
G2	🔍	NA	check warnings
G3	🔍	NA	check warnings
G4	🔍	NA	check warnings
G5	🔍	NA	check warnings
G6	🔍	NA	check warnings
G7	🔍	NA	check warnings
G8	🔍	NA	check warnings
G9	🔍	NA	check warnings
G10	🔍	NA	check warnings
G11	🔍	NA	check warnings
G12	🔍	NA	check warnings
H1	🔍	NA	check warnings
H2	🔍	NA	check warnings
H3	🔍	NA	check warnings
H4	🔍	NA	check warnings
H5	🔍	NA	check warnings
H6	🔍	NA	check warnings
H7	🔍	NA	check warnings
H8	🔍	NA	check warnings
H9	🔍	NA	check warnings
H10	🔍	NA	check warnings
H11	🔍	NA	check warnings
H12	🔍	NA	check warnings