C6 - Screen Contents - Crystal Screen Lite

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Supplemental Information

Screen Description

Crystal Screen Lite

Hampton Research / Crystal Screen Lite

Created 2009-11-16.

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Crystal Screen Lite

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Crystal Screen Lite

Screen Structure

Crystal Screen Lite

Well Count	96
Number of distinct ingredients	28
Internal diversity (score mean dist)	0.935

Screen Composition

More information about screen composition chart.

ingredient type	Crystal Screen Lite
Buffer	62
Organic (non-volatile)	8
Organic (volatile)	7
Polymer	25
Salt	65

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Crystal Screen Lite Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Calcium chloride 0.02 M - Sodium acetate 0.1 M pH 4.6 2-methyl-2,4-pentanediol BCC 15 v/v - 🔍	pH 4.6	100 μl of Sodium acetate pH 4.6 (1 M) 150 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 10 μl of Calcium chloride (2 M) 740 μl of water (100 v/v)
A2	Potassium Sodium tartrate 0.2 M - 🔍	NA	133 μl of Potassium Sodium tartrate (1.5 M) 866 μl of water (100 v/v)
A3	Ammonium di-hydrogen phosphate 0.2 M - 🔍	NA	80 μl of Ammonium di-hydrogen phosphate (2.5 M) 920 μl of water (100 v/v)
A4	tris chloride BCC 0.1 M pH 8.5 Ammonium sulfate 1 M - 🔍	pH 8.5	285 μl of Ammonium sulfate (3.5 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 614 μl of water (100 v/v)
A5	tri-Sodium citrate 0.2 M - HEPES 0.1 M pH 7.5 2-methyl-2,4-pentanediol BCC 15 v/v - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 150 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 125 μl of tri-Sodium citrate (1.6 M) 625 μl of water (100 v/v)
A6	Magnesium chloride 0.2 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 4,000 15 w/v - 🔍	pH 8.5	300 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of Magnesium chloride (2 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 500 μl of water (100 v/v)
A7	Sodium cacodylate 0.1 M pH 6.5 Sodium acetate 0.7 M - 🔍	pH 6.5	100 μl of Sodium cacodylate pH 6.5 (1 M) 233 μl of Sodium acetate (3 M) 666 μl of water (100 v/v)
A8	tri-Sodium citrate 0.2 M - Sodium cacodylate 0.1 M pH 6.5 2-Propanol 15 v/v - 🔍	pH 6.5	100 μl of Sodium cacodylate pH 6.5 (1 M) 125 μl of tri-Sodium citrate (1.6 M) 150 μl of 2-Propanol (100 v/v) 625 μl of water (100 v/v)
A9	Ammonium acetate 0.2 M - tri-Sodium citrate 0.1 M pH 5.6 Polyethylene glycol 4,000 15 w/v - 🔍	pH 5.6	300 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of tri-Sodium citrate pH 5.6 (1 M) 200 μl of Ammonium acetate (1 M) 400 μl of water (100 v/v)
A10	Ammonium acetate 0.2 M - Sodium acetate 0.1 M pH 4.6 Polyethylene glycol 4,000 15 w/v - 🔍	pH 4.6	100 μl of Sodium acetate pH 4.6 (1 M) 300 μl of Polyethylene glycol 4,000 (50 w/v) 200 μl of Ammonium acetate (1 M) 400 μl of water (100 v/v)
A11	tri-Sodium citrate 0.1 M pH 5.6 Ammonium di-hydrogen phosphate 0.5 M - 🔍	pH 5.6	200 μl of Ammonium di-hydrogen phosphate (2.5 M) 100 μl of tri-Sodium citrate pH 5.6 (1 M) 700 μl of water (100 v/v)
A12	Magnesium chloride 0.2 M - HEPES 0.1 M pH 7.5 2-Propanol 15 v/v - 🔍	pH 7.5	100 μl of Magnesium chloride (2 M) 100 μl of HEPES pH 7.5 (1 M) 150 μl of 2-Propanol (100 v/v) 650 μl of water (100 v/v)
B1	tri-Sodium citrate 0.2 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 400 15 v/v - 🔍	pH 8.5	150 μl of Polyethylene glycol 400 (100 v/v) 125 μl of tri-Sodium citrate (1.6 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 625 μl of water (100 v/v)
B2	Calcium chloride 0.2 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 400 14 v/v - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 140 μl of Polyethylene glycol 400 (100 v/v) 100 μl of Calcium chloride (2 M) 660 μl of water (100 v/v)
B3	Ammonium sulfate 0.2 M - Sodium cacodylate 0.1 M pH 6.5 Polyethylene glycol 8,000 15 w/v - 🔍	pH 6.5	100 μl of Sodium cacodylate pH 6.5 (1 M) 57 μl of Ammonium sulfate (3.5 M) 300 μl of Polyethylene glycol 8,000 (50 w/v) 542 μl of water (100 v/v)
B4	HEPES 0.1 M pH 7.5 Lithium sulfate 0.75 M - 🔍	pH 7.5	375 μl of Lithium sulfate (2 M) 100 μl of HEPES pH 7.5 (1 M) 525 μl of water (100 v/v)
B5	Lithium sulfate 0.2 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 4,000 15 w/v - 🔍	pH 8.5	100 μl of Lithium sulfate (2 M) 300 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 500 μl of water (100 v/v)
B6	Magnesium acetate 0.2 M - Sodium cacodylate 0.1 M pH 6.5 Polyethylene glycol 8,000 10 w/v - 🔍	pH 6.5	100 μl of Sodium cacodylate pH 6.5 (1 M) 200 μl of Magnesium acetate (1 M) 200 μl of Polyethylene glycol 8,000 (50 w/v) 500 μl of water (100 v/v)
B7	Ammonium acetate 0.2 M - tris chloride BCC 0.1 M pH 8.5 2-Propanol 15 v/v - 🔍	pH 8.5	150 μl of 2-Propanol (100 v/v) 200 μl of Ammonium acetate (1 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 550 μl of water (100 v/v)
B8	Ammonium sulfate 0.2 M - Sodium acetate 0.1 M pH 4.6 Polyethylene glycol 4,000 12.5 w/v - 🔍	pH 4.6	57 μl of Ammonium sulfate (3.5 M) 100 μl of Sodium acetate pH 4.6 (1 M) 250 μl of Polyethylene glycol 4,000 (50 w/v) 592 μl of water (100 v/v)
B9	Magnesium acetate 0.2 M - Sodium cacodylate 0.1 M pH 6.5 2-methyl-2,4-pentanediol BCC 15 v/v - 🔍	pH 6.5	100 μl of Sodium cacodylate pH 6.5 (1 M) 200 μl of Magnesium acetate (1 M) 150 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 550 μl of water (100 v/v)
B10	Sodium acetate 0.2 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 4,000 15 w/v - 🔍	pH 8.5	300 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 66 μl of Sodium acetate (3 M) 533 μl of water (100 v/v)
B11	Magnesium chloride 0.2 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 400 15 v/v - 🔍	pH 7.5	100 μl of Magnesium chloride (2 M) 100 μl of HEPES pH 7.5 (1 M) 150 μl of Polyethylene glycol 400 (100 v/v) 650 μl of water (100 v/v)
B12	Calcium chloride 0.2 M - Sodium acetate 0.1 M pH 4.6 2-Propanol 10 v/v - 🔍	pH 4.6	100 μl of Sodium acetate pH 4.6 (1 M) 100 μl of Calcium chloride (2 M) 100 μl of 2-Propanol (100 v/v) 700 μl of water (100 v/v)
C1	Imidazole 0.1 M pH 6.5 Sodium acetate 0.5 M - 🔍	pH 6.5	100 μl of Imidazole pH 6.5 (1 M) 166 μl of Sodium acetate (3 M) 733 μl of water (100 v/v)
C2	Ammonium acetate 0.2 M - tri-Sodium citrate 0.1 M pH 5.6 2-methyl-2,4-pentanediol BCC 15 v/v - 🔍	pH 5.6	150 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100 μl of tri-Sodium citrate pH 5.6 (1 M) 200 μl of Ammonium acetate (1 M) 550 μl of water (100 v/v)
C3	tri-Sodium citrate 0.2 M - HEPES 0.1 M pH 7.5 2-Propanol 10 v/v - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 125 μl of tri-Sodium citrate (1.6 M) 100 μl of 2-Propanol (100 v/v) 675 μl of water (100 v/v)
C4	Sodium acetate 0.2 M - Sodium cacodylate 0.1 M pH 6.5 Polyethylene glycol 8,000 15 w/v - 🔍	pH 6.5	100 μl of Sodium cacodylate pH 6.5 (1 M) 300 μl of Polyethylene glycol 8,000 (50 w/v) 66 μl of Sodium acetate (3 M) 533 μl of water (100 v/v)
C5	HEPES 0.1 M pH 7.5 Potassium Sodium tartrate 0.4 M - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 266 μl of Potassium Sodium tartrate (1.5 M) 633 μl of water (100 v/v)
C6	Ammonium sulfate 0.2 M - Polyethylene glycol 8,000 15 w/v - 🔍	NA	57 μl of Ammonium sulfate (3.5 M) 300 μl of Polyethylene glycol 8,000 (50 w/v) 642 μl of water (100 v/v)
C7	Ammonium sulfate 0.2 M - Polyethylene glycol 4,000 15 w/v - 🔍	NA	57 μl of Ammonium sulfate (3.5 M) 300 μl of Polyethylene glycol 4,000 (50 w/v) 642 μl of water (100 v/v)
C8	Ammonium sulfate 1 M - 🔍	NA	285 μl of Ammonium sulfate (3.5 M) 714 μl of water (100 v/v)
C9	Sodium formate 2 M - 🔍	NA	285 μl of Sodium formate (7 M) 714 μl of water (100 v/v)
C10	Sodium formate 1 M - Sodium acetate 0.1 M pH 4.6 🔍	pH 4.6	100 μl of Sodium acetate pH 4.6 (1 M) 142 μl of Sodium formate (7 M) 757 μl of water (100 v/v)
C11	HEPES 0.1 M pH 7.5 Sodium di-hydrogen phosphate 0.4 M - Potassium di-hydrogen phosphate 0.4 M - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 266 μl of Potassium di-hydrogen phosphate (1.5 M) 100 μl of Sodium di-hydrogen phosphate (4 M) 533 μl of water (100 v/v)
C12	tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 8,000 4 w/v - 🔍	pH 8.5	80 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 820 μl of water (100 v/v)
D1	Sodium acetate 0.1 M pH 4.6 Polyethylene glycol 4,000 4 w/v - 🔍	pH 4.6	100 μl of Sodium acetate pH 4.6 (1 M) 80 μl of Polyethylene glycol 4,000 (50 w/v) 820 μl of water (100 v/v)
D2	HEPES 0.1 M pH 7.5 tri-Sodium citrate 0.7 M - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 437 μl of tri-Sodium citrate (1.6 M) 462 μl of water (100 v/v)
D3	HEPES 0.1 M pH 7.5 Polyethylene glycol 400 2 v/v - Ammonium sulfate 1 M - 🔍	pH 7.5	285 μl of Ammonium sulfate (3.5 M) 100 μl of HEPES pH 7.5 (1 M) 20 μl of Polyethylene glycol 400 (100 v/v) 594 μl of water (100 v/v)
D4	tri-Sodium citrate 0.1 M pH 5.6 2-Propanol 10 v/v - Polyethylene glycol 4,000 10 w/v - 🔍	pH 5.6	200 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of tri-Sodium citrate pH 5.6 (1 M) 100 μl of 2-Propanol (100 v/v) 600 μl of water (100 v/v)
D5	HEPES 0.1 M pH 7.5 2-Propanol 5 v/v - Polyethylene glycol 4,000 10 w/v - 🔍	pH 7.5	200 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 50 μl of 2-Propanol (100 v/v) 650 μl of water (100 v/v)
D6	Potassium di-hydrogen phosphate 0.05 M - Polyethylene glycol 8,000 10 w/v - 🔍	NA	200 μl of Polyethylene glycol 8,000 (50 w/v) 33 μl of Potassium di-hydrogen phosphate (1.5 M) 766 μl of water (100 v/v)
D7	Polyethylene glycol 1,500 15 w/v - 🔍	NA	300 μl of Polyethylene glycol 1,500 (50 w/v) 700 μl of water (100 v/v)
D8	Magnesium formate 0.1 M - 🔍	NA	100 μl of Magnesium formate (1 M) 900 μl of water (100 v/v)
D9	Zinc acetate 0.2 M - Sodium cacodylate 0.1 M pH 6.5 Polyethylene glycol 8,000 9 w/v - 🔍	pH 6.5	100 μl of Sodium cacodylate pH 6.5 (1 M) 200 μl of Zinc acetate (1 M) 180 μl of Polyethylene glycol 8,000 (50 w/v) 520 μl of water (100 v/v)
D10	Calcium acetate 0.2 M - Sodium cacodylate 0.1 M pH 6.5 Polyethylene glycol 8,000 9 w/v - 🔍	pH 6.5	100 μl of Sodium cacodylate pH 6.5 (1 M) 200 μl of Calcium acetate (1 M) 180 μl of Polyethylene glycol 8,000 (50 w/v) 520 μl of water (100 v/v)
D11	Ammonium sulfate 1 M - Sodium acetate 0.1 M pH 4.6 🔍	pH 4.6	285 μl of Ammonium sulfate (3.5 M) 100 μl of Sodium acetate pH 4.6 (1 M) 614 μl of water (100 v/v)
D12	tris chloride BCC 0.1 M pH 8.5 Ammonium di-hydrogen phosphate 1 M - 🔍	pH 8.5	400 μl of Ammonium di-hydrogen phosphate (2.5 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 500 μl of water (100 v/v)
E1	Lithium sulfate 0.5 M - Polyethylene glycol 8,000 2 w/v - 🔍	NA	250 μl of Lithium sulfate (2 M) 40 μl of Polyethylene glycol 8,000 (50 w/v) 710 μl of water (100 v/v)
E2	Lithium sulfate 0.5 M - Polyethylene glycol 8,000 7.5 w/v - 🔍	NA	250 μl of Lithium sulfate (2 M) 150 μl of Polyethylene glycol 8,000 (50 w/v) 600 μl of water (100 v/v)
E3	🔍	NA	check warnings
E4	🔍	NA	check warnings
E5	🔍	NA	check warnings
E6	🔍	NA	check warnings
E7	🔍	NA	check warnings
E8	🔍	NA	check warnings
E9	🔍	NA	check warnings
E10	🔍	NA	check warnings
E11	🔍	NA	check warnings
E12	🔍	NA	check warnings
F1	🔍	NA	check warnings
F2	🔍	NA	check warnings
F3	🔍	NA	check warnings
F4	🔍	NA	check warnings
F5	🔍	NA	check warnings
F6	🔍	NA	check warnings
F7	🔍	NA	check warnings
F8	🔍	NA	check warnings
F9	🔍	NA	check warnings
F10	🔍	NA	check warnings
F11	🔍	NA	check warnings
F12	🔍	NA	check warnings
G1	🔍	NA	check warnings
G2	🔍	NA	check warnings
G3	🔍	NA	check warnings
G4	🔍	NA	check warnings
G5	🔍	NA	check warnings
G6	🔍	NA	check warnings
G7	🔍	NA	check warnings
G8	🔍	NA	check warnings
G9	🔍	NA	check warnings
G10	🔍	NA	check warnings
G11	🔍	NA	check warnings
G12	🔍	NA	check warnings
H1	🔍	NA	check warnings
H2	🔍	NA	check warnings
H3	🔍	NA	check warnings
H4	🔍	NA	check warnings
H5	🔍	NA	check warnings
H6	🔍	NA	check warnings
H7	🔍	NA	check warnings
H8	🔍	NA	check warnings
H9	🔍	NA	check warnings
H10	🔍	NA	check warnings
H11	🔍	NA	check warnings
H12	🔍	NA	check warnings