C6 - Classic 10 - Screen Contents

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Supplemental Information

Screen Description

JB Classics / Classic 10

Created 2009-11-16.

Rigaku Recipe XML

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Screen Structure

Well Count	24
Number of distinct ingredients	18
Internal diversity (Score mean dist)	0.92
Internal diversity (Ion mean dist)	0.9
Internal diversity (PEG mean dist)	0.9
Internal diversity (Known pH mean dist)	0.8
Internal diversity (Penalty mean dist)	0.89

Screen Composition

More information about screen composition chart.

Chemical classes bar chart — ingredient type Classic 10

Buffer 37

Cryoprotectant 1

Organic (non-volatile) 1

Salt 29

ingredient type	Classic 10
Buffer	37
Cryoprotectant	1
Organic (non-volatile)	1
Salt	29

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Classic 10 Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Sodium acetate 0.5 M - Imidazole 0.1 M pH 8 🔍 ⚖️	pH 8	100.0 μl of Imidazole pH 8 (1 M) 250.0 μl of Sodium acetate (2 M) 650.0 μl of water (100 v/v)
A2	tri-Sodium citrate 0.7 M - HEPES 0.1 M pH 7.5 🔍 ⚖️	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 350.0 μl of tri-Sodium citrate (2 M) 550.0 μl of water (100 v/v)
A3	Lithium sulfate 0.7 M - tris chloride BCC 0.1 M pH 8.5 🔍 ⚖️	pH 8.5	233.33 μl of Lithium sulfate (3 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 666.67 μl of water (100 v/v)
A4	Potassium Sodium tartrate 0.8 M - HEPES 0.1 M pH 7.5 🔍 ⚖️	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Potassium Sodium tartrate (2 M) 500.0 μl of water (100 v/v)
A5	Ammonium di-hydrogen phosphate 1 M - tri-Sodium citrate 0.1 M pH 5.6 🔍 ⚖️	pH 5.6	333.33 μl of Ammonium di-hydrogen phosphate (3 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 566.67 μl of water (100 v/v)
A6	Ammonium di-hydrogen phosphate 1 M - tris chloride BCC 0.1 M pH 8.5 🔍 ⚖️	pH 8.5	333.33 μl of Ammonium di-hydrogen phosphate (3 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 566.67 μl of water (100 v/v)
B1	Lithium sulfate 1 M - Nickel (II) chloride 0.01 M - tris chloride BCC 0.1 M pH 8.5 🔍 ⚖️	pH 8.5	333.33 μl of Lithium sulfate (3 M) 10.0 μl of Nickel (II) chloride (1 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 556.67 μl of water (100 v/v)
B2	Sodium acetate 1 M - Imidazole 0.1 M pH 8 🔍 ⚖️	pH 8	100.0 μl of Imidazole pH 8 (1 M) 500.0 μl of Sodium acetate (2 M) 400.0 μl of water (100 v/v)
B3	Sodium formate 1 M - Sodium acetate 0.1 M pH 4.6 🔍 ⚖️	pH 4.6	100.0 μl of Sodium acetate pH 4.6 (1 M) 142.86 μl of Sodium formate (7 M) 757.14 μl of water (100 v/v)
B4	Sodium acetate 1.4 M - MES 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 700.0 μl of Sodium acetate (2 M) 200.0 μl of water (100 v/v)
B5	tri-Sodium citrate 1.4 M - HEPES 0.1 M pH 7.5 🔍 ⚖️	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 700.0 μl of tri-Sodium citrate (2 M) 200.0 μl of water (100 v/v)
B6	Lithium sulfate 1.5 M - tris chloride BCC 0.1 M pH 8.5 🔍 ⚖️	pH 8.5	500.0 μl of Lithium sulfate (3 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 400.0 μl of water (100 v/v)
C1	tri-Sodium citrate 1.5 M - 🔍 ⚖️	NA	750.0 μl of tri-Sodium citrate pH 6.5 (2 M) 250.0 μl of water (100 v/v)
C2	Magnesium sulfate 1.6 M - MES 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	800.0 μl of Magnesium sulfate (2 M) 100.0 μl of MES pH 6.5 (1 M) 100.0 μl of water (100 v/v)
C3	Potassium Sodium tartrate 1.6 M - MES 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 800.0 μl of Potassium Sodium tartrate (2 M) 100.0 μl of water (100 v/v)
C4	Ammonium formate 2 M - MES 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	800.0 μl of Ammonium formate (2.5 M) 100.0 μl of MES pH 6.5 (1 M) 100.0 μl of water (100 v/v)
C5	Ammonium di-hydrogen phosphate 2 M - tris chloride BCC 0.1 M pH 8.5 🔍 ⚖️	pH 8.5	666.67 μl of Ammonium di-hydrogen phosphate (3 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 233.33 μl of water (100 v/v)
C6	Sodium formate 2 M - 🔍 ⚖️	NA	285.71 μl of Sodium formate (7 M) 714.29 μl of water (100 v/v)
D1	Magnesium chloride 2 M - tris chloride BCC 0.1 M pH 8.5 🔍 ⚖️	pH 8.5	800.0 μl of Magnesium chloride (2.5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 100.0 μl of water (100 v/v)
D2	Sodium chloride 2 M - Sodium acetate 0.2 M - MES 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 100.0 μl of Sodium acetate (2 M) 400.0 μl of Sodium chloride (5 M) 400.0 μl of water (100 v/v)
D3	Sodium formate 2 M - Sodium acetate 0.1 M pH 4.6 🔍 ⚖️	pH 4.6	100.0 μl of Sodium acetate pH 4.6 (1 M) 285.71 μl of Sodium formate (7 M) 614.29 μl of water (100 v/v)
D4	Glycerol 30 w/v - Ammonium di-hydrogen phosphate 1 M - tris chloride BCC 0.1 M pH 8.5 🔍 ⚖️	pH 8.5	333.33 μl of Ammonium di-hydrogen phosphate (3 M) 375.0 μl of Glycerol (80 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 191.67 μl of water (100 v/v)
D5	Sodium chloride 4 M - HEPES 0.1 M pH 7.5 🔍 ⚖️	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 800.0 μl of Sodium chloride (5 M) 100.0 μl of water (100 v/v)
D6	Sodium formate 3 M - 🔍 ⚖️	NA	428.57 μl of Sodium formate (7 M) 571.43 μl of water (100 v/v)