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Supplemental Information

Screen Description

Classic 10

Classics / Classic 10
Created 2009-11-16.

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Classic 10

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Classic 10

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Screen Structure

Classic 10

Well Count 24
Number of distinct ingredients 18
Internal diversity (score mean dist) 0.919

Screen Composition

More information about screen composition chart.
2025-08-16T09:05:39.977997 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Classic 10
Buffer 37
Cryoprotectant 1
Organic (non-volatile) 1
Salt 29

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Classic 10 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Imidazole 0.1 M pH 8
  • Sodium acetate 0.5 M -
pH 8
100.0 μl of Imidazole pH 8 (1 M)
250.0 μl of Sodium acetate (2 M)
650.0 μl of water (100 v/v)
A2
  • HEPES 0.1 M pH 7.5
  • tri-Sodium citrate 0.7 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
350.0 μl of tri-Sodium citrate (2 M)
550.0 μl of water (100 v/v)
A3
  • tris chloride BCC 0.1 M pH 8.5
  • Lithium sulfate 0.7 M -
pH 8.5
233.3 μl of Lithium sulfate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
666.7 μl of water (100 v/v)
A4
  • HEPES 0.1 M pH 7.5
  • Potassium Sodium tartrate 0.8 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Potassium Sodium tartrate (2 M)
500.0 μl of water (100 v/v)
A5
  • tri-Sodium citrate 0.1 M pH 5.6
  • Ammonium di-hydrogen phosphate 1 M -
pH 5.6
333.3 μl of Ammonium di-hydrogen phosphate (3 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
566.7 μl of water (100 v/v)
A6
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium di-hydrogen phosphate 1 M -
pH 8.5
333.3 μl of Ammonium di-hydrogen phosphate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
566.7 μl of water (100 v/v)
B1
  • Nickel (II) chloride 0.01 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Lithium sulfate 1 M -
pH 8.5
333.3 μl of Lithium sulfate (3 M)
10.0 μl of Nickel (II) chloride (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
556.7 μl of water (100 v/v)
B2
  • Imidazole 0.1 M pH 8
  • Sodium acetate 1 M -
pH 8
100.0 μl of Imidazole pH 8 (1 M)
500.0 μl of Sodium acetate (2 M)
400.0 μl of water (100 v/v)
B3
  • Sodium acetate 0.1 M pH 4.6
  • Sodium formate 1 M -
pH 4.6
100.0 μl of Sodium acetate pH 4.6 (1 M)
142.9 μl of Sodium formate (7 M)
757.1 μl of water (100 v/v)
B4
  • MES 0.1 M pH 6.5
  • Sodium acetate 1.4 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
700.0 μl of Sodium acetate (2 M)
200.0 μl of water (100 v/v)
B5
  • HEPES 0.1 M pH 7.5
  • tri-Sodium citrate 1.4 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
700.0 μl of tri-Sodium citrate (2 M)
200.0 μl of water (100 v/v)
B6
  • tris chloride BCC 0.1 M pH 8.5
  • Lithium sulfate 1.5 M -
pH 8.5
500.0 μl of Lithium sulfate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
C1
  • tri-Sodium citrate 1.5 M -
NA
750.0 μl of tri-Sodium citrate pH 6.5 (2 M)
250.0 μl of water (100 v/v)
C2
  • MES 0.1 M pH 6.5
  • Magnesium sulfate 1.6 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
800.0 μl of Magnesium sulfate (2 M)
100.0 μl of water (100 v/v)
C3
  • MES 0.1 M pH 6.5
  • Potassium Sodium tartrate 1.6 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
800.0 μl of Potassium Sodium tartrate (2 M)
100.0 μl of water (100 v/v)
C4
  • MES 0.1 M pH 6.5
  • Ammonium formate 2 M -
pH 6.5
800.0 μl of Ammonium formate (2.5 M)
100.0 μl of MES pH 6.5 (1 M)
100.0 μl of water (100 v/v)
C5
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium di-hydrogen phosphate 2 M -
pH 8.5
666.7 μl of Ammonium di-hydrogen phosphate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
233.3 μl of water (100 v/v)
C6
  • Sodium formate 2 M -
NA
285.7 μl of Sodium formate (7 M)
714.3 μl of water (100 v/v)
D1
  • tris chloride BCC 0.1 M pH 8.5
  • Magnesium chloride 2 M -
pH 8.5
800.0 μl of Magnesium chloride (2.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
100.0 μl of water (100 v/v)
D2
  • Sodium acetate 0.2 M -
  • MES 0.1 M pH 6.5
  • Sodium chloride 2 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
100.0 μl of Sodium acetate (2 M)
400.0 μl of Sodium chloride (5 M)
400.0 μl of water (100 v/v)
D3
  • Sodium acetate 0.1 M pH 4.6
  • Sodium formate 2 M -
pH 4.6
100.0 μl of Sodium acetate pH 4.6 (1 M)
285.7 μl of Sodium formate (7 M)
614.3 μl of water (100 v/v)
D4
  • tris chloride BCC 0.1 M pH 8.5
  • Glycerol 30 w/v -
  • Ammonium di-hydrogen phosphate 1 M -
pH 8.5
333.3 μl of Ammonium di-hydrogen phosphate (3 M)
375.0 μl of Glycerol (80 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
191.7 μl of water (100 v/v)
D5
  • HEPES 0.1 M pH 7.5
  • Sodium chloride 4 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
800.0 μl of Sodium chloride (5 M)
100.0 μl of water (100 v/v)
D6
  • Sodium formate 3 M -
NA
428.6 μl of Sodium formate (7 M)
571.4 μl of water (100 v/v)