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Supplemental Information

Screen Description

Cryo HTS

Cryos / Cryo HTS
Created 2009-11-16.

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Cryo HTS

    Screen Structure

    Cryo HTS

    Well Count 96
    Number of distinct ingredients 53
    Internal diversity (score mean dist) 0.908

    Screen Composition

    2025-06-13T21:31:48.921677 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Cryo HTS
    Buffer 96
    Cryoprotectant 48
    Organic (non-volatile) 84
    Organic (volatile) 10
    Polymer 59
    Salt 84

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Cryo HTS Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Glycerol 2.8 v/v -
    • Polyethylene glycol 8,000 32 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Cesium chloride 0.2 M -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    28 μl of Glycerol (100 v/v)
    200 μl of Cesium chloride (1 M)
    640 μl of Polyethylene glycol 8,000 (50 w/v)
    32 μl of water (100 v/v)
    A2
    • Glycerol 5 v/v -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Sodium formate 3.2 M -
    pH 5.6
    457 μl of Sodium formate (7 M)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    50 μl of Glycerol (100 v/v)
    392 μl of water (100 v/v)
    A3
    • Glycerol 5 v/v -
    • Polyethylene glycol 4,000 20 w/v -
    NA
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    50 μl of Glycerol (100 v/v)
    550 μl of water (100 v/v)
    A4
    • Glycerol 10 v/v -
    • Polyethylene glycol monomethyl ether 5,000 15 w/v -
    • Xylitol 10 w/v -
    NA
    300 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    333 μl of Xylitol (30 w/v)
    100 μl of Glycerol (100 v/v)
    266 μl of water (100 v/v)
    A5
    • Glycerol 10 v/v -
    • Polyethylene glycol 8,000 28 w/v -
    • MES 0.5 M pH 6.5
    • Ammonium acetate 0.1 M -
    pH 6.5
    20 μl of Ammonium acetate (5 M)
    100 μl of Glycerol (100 v/v)
    166 μl of MES pH 6.5 (3 M)
    560 μl of Polyethylene glycol 8,000 (50 w/v)
    153 μl of water (100 v/v)
    A6
    • Glycerol 10 v/v -
    • Polyethylene glycol 4,000 25 w/v -
    • MES 0.18 M pH 6.5
    • 2-Propanol 4.5 w/v -
    pH 6.5
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Glycerol (100 v/v)
    180 μl of MES pH 6.5 (1 M)
    90 μl of 2-Propanol (50 w/v)
    130 μl of water (100 v/v)
    A7
    • Glycerol 10 v/v -
    • Polyethylene glycol 400 20 v/v -
    • Polyethylene glycol 8,000 10 w/v -
    • tris chloride BCC 0.5 M pH 8.5
    • Sodium chloride 0.1 M -
    pH 8.5
    100 μl of Glycerol (100 v/v)
    166 μl of tris chloride BCC pH 8.5 (3 M)
    200 μl of Polyethylene glycol 400 (100 v/v)
    20 μl of Sodium chloride (5 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    313 μl of water (100 v/v)
    A8
    • Glycerol 10 v/v -
    • Polyethylene glycol 6,000 15 w/v -
    • MES 0.1 M pH 6.5
    • Lithium chloride 0.1 M -
    pH 6.5
    300 μl of Polyethylene glycol 6,000 (50 w/v)
    20 μl of Lithium chloride (5 M)
    100 μl of Glycerol (100 v/v)
    100 μl of MES pH 6.5 (1 M)
    480 μl of water (100 v/v)
    A9
    • Glycerol 15 v/v -
    • Polyethylene glycol monomethyl ether 5,000 15 w/v -
    NA
    300 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    150 μl of Glycerol (100 v/v)
    550 μl of water (100 v/v)
    A10
    • Glycerol 15 v/v -
    • Polyethylene glycol 6,000 20 w/v -
    • MES 0.5 M pH 6.5
    pH 6.5
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    150 μl of Glycerol (100 v/v)
    166 μl of MES pH 6.5 (3 M)
    283 μl of water (100 v/v)
    A11
    • Glycerol 15 v/v -
    • Sodium acetate 0.2 M -
    • Polyethylene glycol 8,000 30 w/v -
    • MES 0.1 M pH 6.5
    pH 6.5
    80 μl of Sodium acetate (2.5 M)
    150 μl of Glycerol (100 v/v)
    100 μl of MES pH 6.5 (1 M)
    600 μl of Polyethylene glycol 8,000 (50 w/v)
    70 μl of water (100 v/v)
    A12
    • Glycerol 15 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium chloride 2.5 M -
    • Lithium sulfate 0.2 M -
    pH 8.5
    66 μl of Lithium sulfate (3 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    150 μl of Glycerol (100 v/v)
    500 μl of Sodium chloride (5 M)
    183 μl of water (100 v/v)
    B1
    • Glycerol 15 v/v -
    • Polyethylene glycol 20,000 15 w/v -
    NA
    300 μl of Polyethylene glycol 20,000 (50 w/v)
    150 μl of Glycerol (100 v/v)
    550 μl of water (100 v/v)
    B2
    • Glycerol 15 v/v -
    • Polyethylene glycol 4,000 35 w/v -
    • tris chloride BCC 0.1 M pH 8
    • tri-Sodium citrate 0.2 M -
    pH 8
    700 μl of Polyethylene glycol 4,000 (50 w/v)
    50 μl of tri-Sodium citrate (4 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    150 μl of Glycerol (100 v/v)
    B3
    • Lithium sulfate 0.5 M -
    • Glycerol 15 v/v -
    • Polyethylene glycol 8,000 15 w/v -
    NA
    166 μl of Lithium sulfate (3 M)
    150 μl of Glycerol (100 v/v)
    300 μl of Polyethylene glycol 8,000 (50 w/v)
    383 μl of water (100 v/v)
    B4
    • Glycerol 20 v/v -
    • Ethylene glycol 16 v/v -
    • Polyethylene glycol 6,000 20 w/v -
    • tris chloride BCC 0.3 M pH 8.5
    • Sodium acetate 0.1 M -
    pH 8.5
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    50 μl of Sodium acetate (2 M)
    160 μl of Ethylene glycol (100 v/v)
    200 μl of Glycerol (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (3 M)
    90 μl of water (100 v/v)
    B5
    • Glycerol 20 v/v -
    • Ammonium sulfate 2 M -
    NA
    666 μl of Ammonium sulfate (3 M)
    200 μl of Glycerol (100 v/v)
    133 μl of water (100 v/v)
    B6
    • Glycerol 20 v/v -
    • Polyethylene glycol 4,000 20 w/v -
    • 2-Propanol 10 v/v -
    • Sodium acetate 0.5 M pH 4.6
    • Sodium chloride 0.1 M -
    pH 4.6
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    166 μl of Sodium acetate pH 4.6 (3 M)
    200 μl of Glycerol (100 v/v)
    100 μl of 2-Propanol (100 v/v)
    20 μl of Sodium chloride (5 M)
    113 μl of water (100 v/v)
    B7
    • Glycerol 20 v/v -
    • MES 0.1 M pH 6.5
    • Ammonium sulfate 2.5 M -
    pH 6.5
    625 μl of Ammonium sulfate (4 M)
    200 μl of Glycerol (100 v/v)
    100 μl of MES pH 6.5 (1 M)
    75 μl of water (100 v/v)
    B8
    • Glycerol 20 v/v -
    • Polyethylene glycol 8,000 15 w/v -
    • MES 0.1 M pH 6.5
    • Zinc acetate 0.16 M -
    pH 6.5
    200 μl of Glycerol (100 v/v)
    100 μl of MES pH 6.5 (1 M)
    80 μl of Zinc acetate (2 M)
    300 μl of Polyethylene glycol 8,000 (50 w/v)
    320 μl of water (100 v/v)
    B9
    • Glycerol 20 v/v -
    • Polyethylene glycol 8,000 15 w/v -
    • tris chloride BCC 0.8 M pH 8.5
    • Sodium chloride 0.92 M -
    pH 8.5
    200 μl of Glycerol (100 v/v)
    266 μl of tris chloride BCC pH 8.5 (3 M)
    184 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol 8,000 (50 w/v)
    49 μl of water (100 v/v)
    B10
    • Glycerol 20 v/v -
    • 2-Propanol 6 v/v -
    • Ammonium sulfate 1.8 M -
    NA
    450 μl of Ammonium sulfate (4 M)
    200 μl of Glycerol (100 v/v)
    60 μl of 2-Propanol (100 v/v)
    290 μl of water (100 v/v)
    B11
    • Glycerol 20 v/v -
    • MES 0.1 M pH 6.5
    • Ammonium sulfate 1.8 M -
    pH 6.5
    450 μl of Ammonium sulfate (4 M)
    200 μl of Glycerol (100 v/v)
    100 μl of MES pH 6.5 (1 M)
    250 μl of water (100 v/v)
    B12
    • Glycerol 20 v/v -
    • Polyethylene glycol 6,000 20 w/v -
    • MES 0.1 M pH 6.5
    pH 6.5
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    200 μl of Glycerol (100 v/v)
    100 μl of MES pH 6.5 (1 M)
    300 μl of water (100 v/v)
    C1
    • Glycerol 20 v/v -
    • Polyethylene glycol 8,000 16 w/v -
    • MES 0.8 M pH 6.5
    • Magnesium acetate 0.16 M -
    pH 6.5
    80 μl of Magnesium acetate (2 M)
    200 μl of Glycerol (100 v/v)
    266 μl of MES pH 6.5 (3 M)
    320 μl of Polyethylene glycol 8,000 (50 w/v)
    133 μl of water (100 v/v)
    C2
    • Glycerol 20 v/v -
    • Polyethylene glycol 8,000 7 w/v -
    • MES 0.1 M pH 6.5
    pH 6.5
    200 μl of Glycerol (100 v/v)
    100 μl of MES pH 6.5 (1 M)
    140 μl of Polyethylene glycol 8,000 (50 w/v)
    560 μl of water (100 v/v)
    C3
    • Glycerol 24 v/v -
    • Ammonium sulfate 2.3 M -
    NA
    575 μl of Ammonium sulfate (4 M)
    240 μl of Glycerol (100 v/v)
    185 μl of water (100 v/v)
    C4
    • Glycerol 25 v/v -
    • Sodium bromide 0.75 M -
    NA
    150 μl of Sodium bromide (5 M)
    250 μl of Glycerol (100 v/v)
    600 μl of water (100 v/v)
    C5
    • Glycerol 25 v/v -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Ammonium di-hydrogen phosphate 1.2 M -
    pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    400 μl of Ammonium di-hydrogen phosphate (3 M)
    250 μl of Glycerol (100 v/v)
    250 μl of water (100 v/v)
    C6
    • Glycerol 25 v/v -
    • Ammonium sulfate 1.5 M -
    NA
    375 μl of Ammonium sulfate (4 M)
    250 μl of Glycerol (100 v/v)
    375 μl of water (100 v/v)
    C7
    • Glycerol 25 v/v -
    • MES 0.1 M pH 6.5
    • Ammonium sulfate 2.2 M -
    pH 6.5
    550 μl of Ammonium sulfate (4 M)
    250 μl of Glycerol (100 v/v)
    100 μl of MES pH 6.5 (1 M)
    100 μl of water (100 v/v)
    C8
    • Glycerol 25 v/v -
    • Sodium Potassium phosphate 0.1 M pH 6.5
    • Ammonium sulfate 2.5 M -
    pH 6.5
    625 μl of Ammonium sulfate (4 M)
    250 μl of Glycerol (100 v/v)
    25 μl of Sodium Potassium phosphate pH 6.5 (4 M)
    100 μl of water (100 v/v)
    C9
    • Glycerol 30 v/v -
    • Ammonium di-hydrogen phosphate 1.26 M -
    NA
    420 μl of Ammonium di-hydrogen phosphate (3 M)
    300 μl of Glycerol (100 v/v)
    280 μl of water (100 v/v)
    C10
    • Glycerol 30 v/v -
    • Sodium acetate 0.1 M pH 4.6
    • Ammonium sulfate 2 M -
    pH 4.6
    500 μl of Ammonium sulfate (4 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    300 μl of Glycerol (100 v/v)
    100 μl of water (100 v/v)
    C11
    • Glycerol 30 v/v -
    • MES 0.5 M pH 6.5
    • Ammonium sulfate 2.3 M -
    pH 6.5
    300 μl of Glycerol (100 v/v)
    166 μl of MES pH 6.5 (3 M)
    460 μl of Ammonium sulfate (5 M)
    73 μl of water (100 v/v)
    C12
    • Glycerol 30 v/v -
    • Ammonium sulfate 2 M -
    NA
    500 μl of Ammonium sulfate (4 M)
    300 μl of Glycerol (100 v/v)
    200 μl of water (100 v/v)
    D1
    • Glycerol 30 v/v -
    • Polyethylene glycol 4,000 15 w/v -
    • 2-Propanol 5 v/v -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    300 μl of Glycerol (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    50 μl of 2-Propanol (100 v/v)
    250 μl of water (100 v/v)
    D2
    • Glycerol 30 v/v -
    • Polyethylene glycol 4,000 20 w/v -
    • 2-Propanol 20 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    300 μl of Glycerol (100 v/v)
    200 μl of 2-Propanol (100 v/v)
    D3
    • Glycerol 30 v/v -
    • MES 0.1 M pH 6.5
    • Ammonium sulfate 1.2 M -
    pH 6.5
    300 μl of Glycerol (100 v/v)
    33 μl of MES pH 6.5 (3 M)
    240 μl of Ammonium sulfate (5 M)
    426 μl of water (100 v/v)
    D4
    • Glycerol 30 v/v -
    • Polyethylene glycol 6,000 15 w/v -
    • tris chloride BCC 0.5 M pH 8.5
    pH 8.5
    300 μl of Polyethylene glycol 6,000 (50 w/v)
    300 μl of Glycerol (100 v/v)
    166 μl of tris chloride BCC pH 8.5 (3 M)
    233 μl of water (100 v/v)
    D5
    • Glycerol 30 v/v -
    • Ammonium sulfate 0.3 M -
    NA
    300 μl of Glycerol (100 v/v)
    60 μl of Ammonium sulfate (5 M)
    640 μl of water (100 v/v)
    D6
    • Glycerol 30 v/v -
    • Polyethylene glycol 1,500 30 w/v -
    • MES 0.05 M pH 6.5
    pH 6.5
    300 μl of Glycerol (100 v/v)
    50 μl of MES pH 6.5 (1 M)
    600 μl of Polyethylene glycol 1,500 (50 w/v)
    50 μl of water (100 v/v)
    D7
    • Glycerol 30 v/v -
    • Sodium Potassium phosphate 0.1 M pH 6.5
    • Ammonium sulfate 1 M -
    pH 6.5
    300 μl of Glycerol (100 v/v)
    200 μl of Ammonium sulfate (5 M)
    25 μl of Sodium Potassium phosphate pH 6.5 (4 M)
    475 μl of water (100 v/v)
    D8
    • Glycerol 30 v/v -
    • Polyethylene glycol 4,000 30 w/v -
    • Sodium acetate 0.1 M pH 4.6
    pH 4.6
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    300 μl of Glycerol (100 v/v)
    D9
    • Glycerol 30 v/v -
    • MES 0.1 M pH 6.5
    • Sodium acetate 1.4 M -
    pH 6.5
    560 μl of Sodium acetate (2.5 M)
    300 μl of Glycerol (100 v/v)
    100 μl of MES pH 6.5 (1 M)
    40 μl of water (100 v/v)
    D10
    • Glycerol 30 v/v -
    • HEPES 0.15 M pH 7.5
    • di-Potassium hydrogen phosphate 0.45 M -
    • Sodium di-hydrogen phosphate 1.6 M -
    pH 7.5
    90 μl of di-Potassium hydrogen phosphate (5 M)
    300 μl of Glycerol (100 v/v)
    150 μl of HEPES pH 7.5 (1 M)
    400 μl of Sodium di-hydrogen phosphate (4 M)
    60 μl of water (100 v/v)
    D11
    • Glycerol 35 v/v -
    • HEPES 0.02 M pH 7.5
    pH 7.5
    350 μl of Glycerol (100 v/v)
    20 μl of HEPES pH 7.5 (1 M)
    630 μl of water (100 v/v)
    D12
    • Glycerol 35 v/v -
    • Polyethylene glycol 6,000 7 w/v -
    • MES 0.05 M pH 6.5
    pH 6.5
    140 μl of Polyethylene glycol 6,000 (50 w/v)
    350 μl of Glycerol (100 v/v)
    50 μl of MES pH 6.5 (1 M)
    460 μl of water (100 v/v)
    E1
    • Polyethylene glycol 200 10 v/v -
    • Polyethylene glycol 3,000 24 w/v -
    • tri-Sodium citrate 0.1 M pH 5.6
    pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    480 μl of Polyethylene glycol 3,000 (50 w/v)
    100 μl of Polyethylene glycol 200 (100 v/v)
    320 μl of water (100 v/v)
    E2
    • Polyethylene glycol 200 20 v/v -
    • Polyethylene glycol 4,000 20 w/v -
    • 2-Propanol 10 v/v -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    200 μl of Polyethylene glycol 200 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    100 μl of 2-Propanol (100 v/v)
    200 μl of water (100 v/v)
    E3
    • Polyethylene glycol 200 30 v/v -
    • Polyethylene glycol 3,000 5 w/v -
    • MES 0.1 M pH 7.5
    pH 7.5
    100 μl of Polyethylene glycol 3,000 (50 w/v)
    33 μl of MES pH 7.5 (3 M)
    300 μl of Polyethylene glycol 200 (100 v/v)
    566 μl of water (100 v/v)
    E4
    • Polyethylene glycol 300 40 v/v -
    • Polyethylene glycol 1,000 5 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of Polyethylene glycol 1,000 (50 w/v)
    400 μl of water (100 v/v)
    E5
    • Polyethylene glycol 400 20 v/v -
    • Polyethylene glycol 4,000 30 w/v -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Ammonium acetate 0.05 M -
    pH 5.6
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    10 μl of Ammonium acetate (5 M)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    200 μl of Polyethylene glycol 400 (100 v/v)
    90 μl of water (100 v/v)
    E6
    • Polyethylene glycol 400 30 v/v -
    • Polyethylene glycol 20,000 11 w/v -
    • di-Potassium hydrogen phosphate 0.1 M pH 8
    pH 8
    220 μl of Polyethylene glycol 20,000 (50 w/v)
    100 μl of di-Potassium hydrogen phosphate pH 8 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    380 μl of water (100 v/v)
    E7
    • Polyethylene glycol 400 10 v/v -
    • Polyethylene glycol monomethyl ether 5,000 20 w/v -
    NA
    400 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    100 μl of Polyethylene glycol 400 (100 v/v)
    500 μl of water (100 v/v)
    E8
    • Polyethylene glycol 400 34 v/v -
    • HEPES 0.1 M pH 7.5
    • Sodium chloride 0.1 M -
    • Calcium chloride 0.2 M -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    40 μl of Calcium chloride (5 M)
    340 μl of Polyethylene glycol 400 (100 v/v)
    20 μl of Sodium chloride (5 M)
    500 μl of water (100 v/v)
    E9
    • Polyethylene glycol 400 40 v/v -
    • Polyethylene glycol 3,000 5 w/v -
    • MES 0.1 M pH 6.5
    pH 6.5
    100 μl of Polyethylene glycol 3,000 (50 w/v)
    100 μl of MES pH 6.5 (1 M)
    400 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of water (100 v/v)
    E10
    • Polyethylene glycol 600 35 v/v -
    • PIPES 0.1 M pH 7
    • Sodium chloride 0.1 M -
    pH 7
    700 μl of Polyethylene glycol 600 (50 v/v)
    100 μl of PIPES pH 7 (1 M)
    20 μl of Sodium chloride (5 M)
    180 μl of water (100 v/v)
    E11
    • Polyethylene glycol 1,000 30 w/v -
    • Sodium phosphate citrate 0.1 M pH 4.4
    • Lithium sulfate 0.11 M -
    pH 4.4
    36 μl of Lithium sulfate (3 M)
    100 μl of Sodium phosphate citrate pH 4.4 (1 M)
    600 μl of Polyethylene glycol 1,000 (50 w/v)
    263 μl of water (100 v/v)
    E12
    • Polyethylene glycol 1,500 40 w/v -
    • ADA 0.1 M pH 6
    pH 6
    800 μl of Polyethylene glycol 1,500 (50 w/v)
    100 μl of ADA pH 6 (1 M)
    100 μl of water (100 v/v)
    F1
    • Polyethylene glycol 1,500 17 w/v -
    • 2-methyl-2,4-pentanediol BCC 10 v/v -
    • PIPES 0.5 M pH 7
    pH 7
    500 μl of PIPES pH 7 (1 M)
    340 μl of Polyethylene glycol 1,500 (50 w/v)
    100 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    60 μl of water (100 v/v)
    F2
    • Polyethylene glycol 3,350 35 w/v -
    • Potassium thiocyanate 0.3 M -
    NA
    37 μl of Potassium thiocyanate (8 M)
    700 μl of Polyethylene glycol 3,350 (50 w/v)
    262 μl of water (100 v/v)
    F3
    • Polyethylene glycol 4,000 25 w/v -
    • 2-methyl-2,4-pentanediol BCC 18 v/v -
    • Sodium acetate 0.1 M pH 4.6
    • Ammonium sulfate 0.2 M -
    pH 4.6
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    66 μl of Ammonium sulfate (3 M)
    180 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    153 μl of water (100 v/v)
    F4
    • Polyethylene glycol 4,000 25 w/v -
    • Ethylene glycol 30 v/v -
    NA
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    300 μl of Ethylene glycol (100 v/v)
    200 μl of water (100 v/v)
    F5
    • Polyethylene glycol 4,000 20 w/v -
    • 2-methyl-2,4-pentanediol BCC 20 v/v -
    • 2-Propanol 10 v/v -
    • tri-Sodium citrate 0.5 M pH 5.6
    pH 5.6
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    250 μl of tri-Sodium citrate pH 5.6 (2 M)
    100 μl of 2-Propanol (100 v/v)
    200 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    50 μl of water (100 v/v)
    F6
    • Polyethylene glycol 4,000 10 w/v -
    • Ethylene glycol 20 v/v -
    • ADA 0.055 M pH 6
    • Sodium chloride 0.105 M -
    pH 6
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    200 μl of Ethylene glycol (100 v/v)
    21 μl of Sodium chloride (5 M)
    55 μl of ADA pH 6 (1 M)
    524 μl of water (100 v/v)
    F7
    • Polyethylene glycol 400 2 v/v -
    • 2-methyl-2,4-pentanediol BCC 25 v/v -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    20 μl of Polyethylene glycol 400 (100 v/v)
    250 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    630 μl of water (100 v/v)
    F8
    • Polyethylene glycol 8,000 20 w/v -
    • 2-methyl-2,4-pentanediol BCC 20 v/v -
    • HEPES 0.1 M pH 7.5
    • Rubidium bromide 0.2 M -
    pH 7.5
    200 μl of Rubidium bromide (1 M)
    100 μl of HEPES pH 7.5 (1 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of water (100 v/v)
    F9
    • Polyethylene glycol 8,000 28 w/v -
    • Glycine 0.1 M pH 9.5
    • Calcium chloride 0.01 M -
    pH 9.5
    50 μl of Glycine pH 9.5 (2 M)
    2 μl of Calcium chloride (5 M)
    560 μl of Polyethylene glycol 8,000 (50 w/v)
    388 μl of water (100 v/v)
    F10
    • Polyethylene glycol 8,000 10 w/v -
    • 2-methyl-2,4-pentanediol BCC 15 v/v -
    • MES 0.1 M pH 6.5
    • Ammonium sulfate 0.055 M -
    pH 6.5
    18 μl of Ammonium sulfate (3 M)
    33 μl of MES pH 6.5 (3 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    150 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    598 μl of water (100 v/v)
    F11
    • Polyethylene glycol 8,000 25 w/v -
    • Ethylene glycol 30 v/v -
    • ADA 0.15 M pH 6
    • Ammonium sulfate 0.08 M -
    pH 6
    300 μl of Ethylene glycol (100 v/v)
    26 μl of Ammonium sulfate (3 M)
    500 μl of Polyethylene glycol 8,000 (50 w/v)
    150 μl of ADA pH 6 (1 M)
    23 μl of water (100 v/v)
    F12
    • Polyethylene glycol 20,000 10 w/v -
    • Ethylene glycol 5.6 v/v -
    • 2,3-Butanediol 30 v/v -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    300 μl of 2,3-Butanediol (100 v/v)
    200 μl of Polyethylene glycol 20,000 (50 w/v)
    112 μl of Ethylene glycol (50 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    288 μl of water (100 v/v)
    G1
    • Ethylene glycol 20 v/v -
    • HEPES 0.1 M pH 7.5
    • Lithium sulfate 1.6 M -
    pH 7.5
    533 μl of Lithium sulfate (3 M)
    200 μl of Ethylene glycol (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    166 μl of water (100 v/v)
    G2
    • Ethylene glycol 25 v/v -
    • HEPES 0.1 M pH 7.5
    • Sodium Potassium phosphate 0.2 M -
    pH 7.5
    250 μl of Ethylene glycol (100 v/v)
    200 μl of Sodium Potassium phosphate pH 7 (1 M)
    100 μl of HEPES pH 7.5 (1 M)
    450 μl of water (100 v/v)
    G3
    • Ethylene glycol 25 v/v -
    • HEPES 0.1 M pH 7.5
    • Potassium Sodium tartrate 1 M -
    pH 7.5
    250 μl of Ethylene glycol (100 v/v)
    500 μl of Potassium Sodium tartrate (2 M)
    100 μl of HEPES pH 7.5 (1 M)
    150 μl of water (100 v/v)
    G4
    • Ethylene glycol 40 v/v -
    • Sodium acetate 0.1 M pH 4.6
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    400 μl of Ethylene glycol (100 v/v)
    500 μl of water (100 v/v)
    G5
    • Ethylene glycol 40 v/v -
    • tris chloride BCC 0.1 M pH 7.5
    pH 7.5
    33 μl of tris chloride BCC pH 7.5 (3 M)
    400 μl of Ethylene glycol (100 v/v)
    566 μl of water (100 v/v)
    G6
    • Ethylene glycol 40 v/v -
    • Imidazole 0.1 M pH 9
    • Calcium acetate 0.2 M -
    pH 9
    400 μl of Ethylene glycol (100 v/v)
    100 μl of Imidazole pH 9 (1 M)
    100 μl of Calcium acetate (2 M)
    400 μl of water (100 v/v)
    G7
    • Ethylene glycol 40 v/v -
    • MES 0.1 M pH 6.5
    • Zinc acetate 0.2 M -
    pH 6.5
    400 μl of Ethylene glycol (100 v/v)
    100 μl of Zinc acetate (2 M)
    33 μl of MES pH 6.5 (3 M)
    466 μl of water (100 v/v)
    G8
    • Ethylene glycol 50 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Magnesium chloride 0.2 M -
    pH 8.5
    500 μl of Ethylene glycol (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    80 μl of Magnesium chloride pH 7 (2.5 M)
    320 μl of water (100 v/v)
    G9
    • Ethylene glycol 50 v/v -
    • HEPES 0.1 M pH 7.5
    • Lithium sulfate 0.2 M -
    pH 7.5
    66 μl of Lithium sulfate (3 M)
    500 μl of Ethylene glycol (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    333 μl of water (100 v/v)
    G10
    • 2-Propanol 35 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    350 μl of 2-Propanol (100 v/v)
    550 μl of water (100 v/v)
    G11
    • 2-Propanol 35 v/v -
    • Sodium acetate 0.1 M pH 4.6
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    350 μl of 2-Propanol (100 v/v)
    550 μl of water (100 v/v)
    G12
    • 2-Propanol 35 v/v -
    • Imidazole 0.1 M pH 9
    • Zinc acetate 0.2 M -
    pH 9
    100 μl of Imidazole pH 9 (1 M)
    100 μl of Zinc acetate (2 M)
    350 μl of 2-Propanol (100 v/v)
    450 μl of water (100 v/v)
    H1
    • 1,2-Propanediol 40 v/v -
    • Imidazole 0.1 M pH 9
    pH 9
    100 μl of Imidazole pH 9 (1 M)
    400 μl of 1,2-Propanediol (100 v/v)
    500 μl of water (100 v/v)
    H2
    • 1,2-Propanediol 40 v/v -
    • Sodium acetate 0.1 M pH 4.6
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    400 μl of 1,2-Propanediol (100 v/v)
    500 μl of water (100 v/v)
    H3
    • 1,2-Propanediol 40 v/v -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Sodium chloride 0.2 M -
    pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    400 μl of 1,2-Propanediol (100 v/v)
    200 μl of Sodium chloride (1 M)
    300 μl of water (100 v/v)
    H4
    • 1,2-Propanediol 40 v/v -
    • Sodium acetate 0.1 M pH 4.6
    • Calcium acetate 0.5 M -
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    250 μl of Calcium acetate (2 M)
    400 μl of 1,2-Propanediol (100 v/v)
    250 μl of water (100 v/v)
    H5
    • 2-methyl-2,4-pentanediol BCC 35 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    550 μl of water (100 v/v)
    H6
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    • Sodium acetate 0.1 M pH 4.6
    • Calcium chloride 0.2 M -
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    40 μl of Calcium chloride (5 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    560 μl of water (100 v/v)
    H7
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    • HEPES 0.1 M pH 7.5
    • tri-Sodium citrate 0.2 M -
    pH 7.5
    40 μl of tri-Sodium citrate (5 M)
    100 μl of HEPES pH 7.5 (1 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    560 μl of water (100 v/v)
    H8
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Ammonium acetate 0.2 M -
    pH 5.6
    40 μl of Ammonium acetate (5 M)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    560 μl of water (100 v/v)
    H9
    • 2-methyl-2,4-pentanediol BCC 35 v/v -
    • MES 0.1 M pH 6.5
    • Ammonium sulfate 0.2 M -
    pH 6.5
    66 μl of Ammonium sulfate (3 M)
    33 μl of MES pH 6.5 (3 M)
    350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    550 μl of water (100 v/v)
    H10
    • 2-methyl-2,4-pentanediol BCC 40 v/v -
    • tri-Sodium citrate 0.1 M pH 5.6
    pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    500 μl of water (100 v/v)
    H11
    • 2-methyl-2,4-pentanediol BCC 40 v/v -
    • MES 0.1 M pH 6.5
    • Ammonium sulfate 0.2 M -
    pH 6.5
    66 μl of Ammonium sulfate (3 M)
    33 μl of MES pH 6.5 (3 M)
    400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    500 μl of water (100 v/v)
    H12
    • 2-methyl-2,4-pentanediol BCC 40 v/v -
    • Imidazole 0.1 M pH 9
    • Magnesium chloride 0.2 M -
    pH 9
    100 μl of Imidazole pH 9 (1 M)
    80 μl of Magnesium chloride pH 7 (2.5 M)
    400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    420 μl of water (100 v/v)