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Supplemental Information

Screen Description

Cryo HTS

Cryos / Cryo HTS
Created 2009-11-16.

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Cryo HTS

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Screen Structure

Cryo HTS

Well Count 96
Number of distinct ingredients 53
Internal diversity (score mean dist) 0.908

Screen Composition

2025-08-16T09:05:41.392172 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Cryo HTS
Buffer 96
Cryoprotectant 48
Organic (non-volatile) 84
Organic (volatile) 10
Polymer 59
Salt 84

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Cryo HTS Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Glycerol 2.8 v/v -
  • Polyethylene glycol 8,000 32 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Cesium chloride 0.2 M -
pH 8.5
200.0 μl of Cesium chloride (1 M)
28.0 μl of Glycerol (100 v/v)
640.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
32.0 μl of water (100 v/v)
A2
  • Glycerol 5 v/v -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Sodium formate 3.2 M -
pH 5.6
50.0 μl of Glycerol (100 v/v)
457.1 μl of Sodium formate (7 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
392.9 μl of water (100 v/v)
A3
  • Glycerol 5 v/v -
  • Polyethylene glycol 4,000 20 w/v -
NA
50.0 μl of Glycerol (100 v/v)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
550.0 μl of water (100 v/v)
A4
  • Glycerol 10 v/v -
  • Polyethylene glycol monomethyl ether 5,000 15 w/v -
  • Xylitol 10 w/v -
NA
100.0 μl of Glycerol (100 v/v)
300.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
333.3 μl of Xylitol (30 w/v)
266.7 μl of water (100 v/v)
A5
  • Glycerol 10 v/v -
  • Polyethylene glycol 8,000 28 w/v -
  • MES 0.5 M pH 6.5
  • Ammonium acetate 0.1 M -
pH 6.5
20.0 μl of Ammonium acetate (5 M)
100.0 μl of Glycerol (100 v/v)
166.7 μl of MES pH 6.5 (3 M)
560.0 μl of Polyethylene glycol 8,000 (50 w/v)
153.3 μl of water (100 v/v)
A6
  • Glycerol 10 v/v -
  • Polyethylene glycol 4,000 25 w/v -
  • MES 0.18 M pH 6.5
  • 2-Propanol 4.5 w/v -
pH 6.5
90.0 μl of 2-Propanol (50 w/v)
100.0 μl of Glycerol (100 v/v)
180.0 μl of MES pH 6.5 (1 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
130.0 μl of water (100 v/v)
A7
  • Glycerol 10 v/v -
  • Polyethylene glycol 400 20 v/v -
  • Polyethylene glycol 8,000 10 w/v -
  • tris chloride BCC 0.5 M pH 8.5
  • Sodium chloride 0.1 M -
pH 8.5
100.0 μl of Glycerol (100 v/v)
200.0 μl of Polyethylene glycol 400 (100 v/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
20.0 μl of Sodium chloride (5 M)
166.7 μl of tris chloride BCC pH 8.5 (3 M)
313.3 μl of water (100 v/v)
A8
  • Glycerol 10 v/v -
  • Polyethylene glycol 6,000 15 w/v -
  • MES 0.1 M pH 6.5
  • Lithium chloride 0.1 M -
pH 6.5
100.0 μl of Glycerol (100 v/v)
20.0 μl of Lithium chloride (5 M)
100.0 μl of MES pH 6.5 (1 M)
300.0 μl of Polyethylene glycol 6,000 (50 w/v)
480.0 μl of water (100 v/v)
A9
  • Glycerol 15 v/v -
  • Polyethylene glycol monomethyl ether 5,000 15 w/v -
NA
150.0 μl of Glycerol (100 v/v)
300.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
550.0 μl of water (100 v/v)
A10
  • Glycerol 15 v/v -
  • Polyethylene glycol 6,000 20 w/v -
  • MES 0.5 M pH 6.5
pH 6.5
150.0 μl of Glycerol (100 v/v)
166.7 μl of MES pH 6.5 (3 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
283.3 μl of water (100 v/v)
A11
  • Glycerol 15 v/v -
  • Sodium acetate 0.2 M -
  • Polyethylene glycol 8,000 30 w/v -
  • MES 0.1 M pH 6.5
pH 6.5
150.0 μl of Glycerol (100 v/v)
100.0 μl of MES pH 6.5 (1 M)
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
80.0 μl of Sodium acetate (2.5 M)
70.0 μl of water (100 v/v)
A12
  • Glycerol 15 v/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Sodium chloride 2.5 M -
  • Lithium sulfate 0.2 M -
pH 8.5
150.0 μl of Glycerol (100 v/v)
66.7 μl of Lithium sulfate (3 M)
500.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
183.3 μl of water (100 v/v)
B1
  • Glycerol 15 v/v -
  • Polyethylene glycol 20,000 15 w/v -
NA
150.0 μl of Glycerol (100 v/v)
300.0 μl of Polyethylene glycol 20,000 (50 w/v)
550.0 μl of water (100 v/v)
B2
  • Glycerol 15 v/v -
  • Polyethylene glycol 4,000 35 w/v -
  • tris chloride BCC 0.1 M pH 8
  • tri-Sodium citrate 0.2 M -
pH 8
150.0 μl of Glycerol (100 v/v)
700.0 μl of Polyethylene glycol 4,000 (50 w/v)
50.0 μl of tri-Sodium citrate (4 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
B3
  • Lithium sulfate 0.5 M -
  • Glycerol 15 v/v -
  • Polyethylene glycol 8,000 15 w/v -
NA
150.0 μl of Glycerol (100 v/v)
166.7 μl of Lithium sulfate (3 M)
300.0 μl of Polyethylene glycol 8,000 (50 w/v)
383.3 μl of water (100 v/v)
B4
  • Glycerol 20 v/v -
  • Ethylene glycol 16 v/v -
  • Polyethylene glycol 6,000 20 w/v -
  • tris chloride BCC 0.3 M pH 8.5
  • Sodium acetate 0.1 M -
pH 8.5
160.0 μl of Ethylene glycol (100 v/v)
200.0 μl of Glycerol (100 v/v)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
50.0 μl of Sodium acetate (2 M)
100.0 μl of tris chloride BCC pH 8.5 (3 M)
90.0 μl of water (100 v/v)
B5
  • Glycerol 20 v/v -
  • Ammonium sulfate 2 M -
NA
666.7 μl of Ammonium sulfate (3 M)
200.0 μl of Glycerol (100 v/v)
133.3 μl of water (100 v/v)
B6
  • Glycerol 20 v/v -
  • Polyethylene glycol 4,000 20 w/v -
  • 2-Propanol 10 v/v -
  • Sodium acetate 0.5 M pH 4.6
  • Sodium chloride 0.1 M -
pH 4.6
100.0 μl of 2-Propanol (100 v/v)
200.0 μl of Glycerol (100 v/v)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
166.7 μl of Sodium acetate pH 4.6 (3 M)
20.0 μl of Sodium chloride (5 M)
113.3 μl of water (100 v/v)
B7
  • Glycerol 20 v/v -
  • MES 0.1 M pH 6.5
  • Ammonium sulfate 2.5 M -
pH 6.5
625.0 μl of Ammonium sulfate (4 M)
200.0 μl of Glycerol (100 v/v)
100.0 μl of MES pH 6.5 (1 M)
75.0 μl of water (100 v/v)
B8
  • Glycerol 20 v/v -
  • Polyethylene glycol 8,000 15 w/v -
  • MES 0.1 M pH 6.5
  • Zinc acetate 0.16 M -
pH 6.5
200.0 μl of Glycerol (100 v/v)
100.0 μl of MES pH 6.5 (1 M)
300.0 μl of Polyethylene glycol 8,000 (50 w/v)
80.0 μl of Zinc acetate (2 M)
320.0 μl of water (100 v/v)
B9
  • Glycerol 20 v/v -
  • Polyethylene glycol 8,000 15 w/v -
  • tris chloride BCC 0.8 M pH 8.5
  • Sodium chloride 0.92 M -
pH 8.5
200.0 μl of Glycerol (100 v/v)
300.0 μl of Polyethylene glycol 8,000 (50 w/v)
184.0 μl of Sodium chloride (5 M)
266.7 μl of tris chloride BCC pH 8.5 (3 M)
49.3 μl of water (100 v/v)
B10
  • Glycerol 20 v/v -
  • 2-Propanol 6 v/v -
  • Ammonium sulfate 1.8 M -
NA
60.0 μl of 2-Propanol (100 v/v)
450.0 μl of Ammonium sulfate (4 M)
200.0 μl of Glycerol (100 v/v)
290.0 μl of water (100 v/v)
B11
  • Glycerol 20 v/v -
  • MES 0.1 M pH 6.5
  • Ammonium sulfate 1.8 M -
pH 6.5
450.0 μl of Ammonium sulfate (4 M)
200.0 μl of Glycerol (100 v/v)
100.0 μl of MES pH 6.5 (1 M)
250.0 μl of water (100 v/v)
B12
  • Glycerol 20 v/v -
  • Polyethylene glycol 6,000 20 w/v -
  • MES 0.1 M pH 6.5
pH 6.5
200.0 μl of Glycerol (100 v/v)
100.0 μl of MES pH 6.5 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
300.0 μl of water (100 v/v)
C1
  • Glycerol 20 v/v -
  • Polyethylene glycol 8,000 16 w/v -
  • MES 0.8 M pH 6.5
  • Magnesium acetate 0.16 M -
pH 6.5
200.0 μl of Glycerol (100 v/v)
266.7 μl of MES pH 6.5 (3 M)
80.0 μl of Magnesium acetate (2 M)
320.0 μl of Polyethylene glycol 8,000 (50 w/v)
133.3 μl of water (100 v/v)
C2
  • Glycerol 20 v/v -
  • Polyethylene glycol 8,000 7 w/v -
  • MES 0.1 M pH 6.5
pH 6.5
200.0 μl of Glycerol (100 v/v)
100.0 μl of MES pH 6.5 (1 M)
140.0 μl of Polyethylene glycol 8,000 (50 w/v)
560.0 μl of water (100 v/v)
C3
  • Glycerol 24 v/v -
  • Ammonium sulfate 2.3 M -
NA
575.0 μl of Ammonium sulfate (4 M)
240.0 μl of Glycerol (100 v/v)
185.0 μl of water (100 v/v)
C4
  • Glycerol 25 v/v -
  • Sodium bromide 0.75 M -
NA
250.0 μl of Glycerol (100 v/v)
150.0 μl of Sodium bromide (5 M)
600.0 μl of water (100 v/v)
C5
  • Glycerol 25 v/v -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Ammonium di-hydrogen phosphate 1.2 M -
pH 5.6
400.0 μl of Ammonium di-hydrogen phosphate (3 M)
250.0 μl of Glycerol (100 v/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
250.0 μl of water (100 v/v)
C6
  • Glycerol 25 v/v -
  • Ammonium sulfate 1.5 M -
NA
375.0 μl of Ammonium sulfate (4 M)
250.0 μl of Glycerol (100 v/v)
375.0 μl of water (100 v/v)
C7
  • Glycerol 25 v/v -
  • MES 0.1 M pH 6.5
  • Ammonium sulfate 2.2 M -
pH 6.5
550.0 μl of Ammonium sulfate (4 M)
250.0 μl of Glycerol (100 v/v)
100.0 μl of MES pH 6.5 (1 M)
100.0 μl of water (100 v/v)
C8
  • Glycerol 25 v/v -
  • Sodium Potassium phosphate 0.1 M pH 6.5
  • Ammonium sulfate 2.5 M -
pH 6.5
625.0 μl of Ammonium sulfate (4 M)
250.0 μl of Glycerol (100 v/v)
25.0 μl of Sodium Potassium phosphate pH 6.5 (4 M)
100.0 μl of water (100 v/v)
C9
  • Glycerol 30 v/v -
  • Ammonium di-hydrogen phosphate 1.26 M -
NA
420.0 μl of Ammonium di-hydrogen phosphate (3 M)
300.0 μl of Glycerol (100 v/v)
280.0 μl of water (100 v/v)
C10
  • Glycerol 30 v/v -
  • Sodium acetate 0.1 M pH 4.6
  • Ammonium sulfate 2 M -
pH 4.6
500.0 μl of Ammonium sulfate (4 M)
300.0 μl of Glycerol (100 v/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
100.0 μl of water (100 v/v)
C11
  • Glycerol 30 v/v -
  • MES 0.5 M pH 6.5
  • Ammonium sulfate 2.3 M -
pH 6.5
460.0 μl of Ammonium sulfate (5 M)
300.0 μl of Glycerol (100 v/v)
166.7 μl of MES pH 6.5 (3 M)
73.3 μl of water (100 v/v)
C12
  • Glycerol 30 v/v -
  • Ammonium sulfate 2 M -
NA
500.0 μl of Ammonium sulfate (4 M)
300.0 μl of Glycerol (100 v/v)
200.0 μl of water (100 v/v)
D1
  • Glycerol 30 v/v -
  • Polyethylene glycol 4,000 15 w/v -
  • 2-Propanol 5 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
50.0 μl of 2-Propanol (100 v/v)
300.0 μl of Glycerol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
250.0 μl of water (100 v/v)
D2
  • Glycerol 30 v/v -
  • Polyethylene glycol 4,000 20 w/v -
  • 2-Propanol 20 v/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
200.0 μl of 2-Propanol (100 v/v)
300.0 μl of Glycerol (100 v/v)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
D3
  • Glycerol 30 v/v -
  • MES 0.1 M pH 6.5
  • Ammonium sulfate 1.2 M -
pH 6.5
240.0 μl of Ammonium sulfate (5 M)
300.0 μl of Glycerol (100 v/v)
33.3 μl of MES pH 6.5 (3 M)
426.7 μl of water (100 v/v)
D4
  • Glycerol 30 v/v -
  • Polyethylene glycol 6,000 15 w/v -
  • tris chloride BCC 0.5 M pH 8.5
pH 8.5
300.0 μl of Glycerol (100 v/v)
300.0 μl of Polyethylene glycol 6,000 (50 w/v)
166.7 μl of tris chloride BCC pH 8.5 (3 M)
233.3 μl of water (100 v/v)
D5
  • Glycerol 30 v/v -
  • Ammonium sulfate 0.3 M -
NA
60.0 μl of Ammonium sulfate (5 M)
300.0 μl of Glycerol (100 v/v)
640.0 μl of water (100 v/v)
D6
  • Glycerol 30 v/v -
  • Polyethylene glycol 1,500 30 w/v -
  • MES 0.05 M pH 6.5
pH 6.5
300.0 μl of Glycerol (100 v/v)
50.0 μl of MES pH 6.5 (1 M)
600.0 μl of Polyethylene glycol 1,500 (50 w/v)
50.0 μl of water (100 v/v)
D7
  • Glycerol 30 v/v -
  • Sodium Potassium phosphate 0.1 M pH 6.5
  • Ammonium sulfate 1 M -
pH 6.5
200.0 μl of Ammonium sulfate (5 M)
300.0 μl of Glycerol (100 v/v)
25.0 μl of Sodium Potassium phosphate pH 6.5 (4 M)
475.0 μl of water (100 v/v)
D8
  • Glycerol 30 v/v -
  • Polyethylene glycol 4,000 30 w/v -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
300.0 μl of Glycerol (100 v/v)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
D9
  • Glycerol 30 v/v -
  • MES 0.1 M pH 6.5
  • Sodium acetate 1.4 M -
pH 6.5
300.0 μl of Glycerol (100 v/v)
100.0 μl of MES pH 6.5 (1 M)
560.0 μl of Sodium acetate (2.5 M)
40.0 μl of water (100 v/v)
D10
  • Glycerol 30 v/v -
  • HEPES 0.15 M pH 7.5
  • di-Potassium hydrogen phosphate 0.45 M -
  • Sodium di-hydrogen phosphate 1.6 M -
pH 7.5
300.0 μl of Glycerol (100 v/v)
150.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Sodium di-hydrogen phosphate (4 M)
90.0 μl of di-Potassium hydrogen phosphate (5 M)
60.0 μl of water (100 v/v)
D11
  • Glycerol 35 v/v -
  • HEPES 0.02 M pH 7.5
pH 7.5
350.0 μl of Glycerol (100 v/v)
20.0 μl of HEPES pH 7.5 (1 M)
630.0 μl of water (100 v/v)
D12
  • Glycerol 35 v/v -
  • Polyethylene glycol 6,000 7 w/v -
  • MES 0.05 M pH 6.5
pH 6.5
350.0 μl of Glycerol (100 v/v)
50.0 μl of MES pH 6.5 (1 M)
140.0 μl of Polyethylene glycol 6,000 (50 w/v)
460.0 μl of water (100 v/v)
E1
  • Polyethylene glycol 200 10 v/v -
  • Polyethylene glycol 3,000 24 w/v -
  • tri-Sodium citrate 0.1 M pH 5.6
pH 5.6
100.0 μl of Polyethylene glycol 200 (100 v/v)
480.0 μl of Polyethylene glycol 3,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
320.0 μl of water (100 v/v)
E2
  • Polyethylene glycol 200 20 v/v -
  • Polyethylene glycol 4,000 20 w/v -
  • 2-Propanol 10 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of 2-Propanol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 200 (100 v/v)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
200.0 μl of water (100 v/v)
E3
  • Polyethylene glycol 200 30 v/v -
  • Polyethylene glycol 3,000 5 w/v -
  • MES 0.1 M pH 7.5
pH 7.5
33.3 μl of MES pH 7.5 (3 M)
300.0 μl of Polyethylene glycol 200 (100 v/v)
100.0 μl of Polyethylene glycol 3,000 (50 w/v)
566.7 μl of water (100 v/v)
E4
  • Polyethylene glycol 300 40 v/v -
  • Polyethylene glycol 1,000 5 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
100.0 μl of Polyethylene glycol 1,000 (50 w/v)
400.0 μl of Polyethylene glycol 300 (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
E5
  • Polyethylene glycol 400 20 v/v -
  • Polyethylene glycol 4,000 30 w/v -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Ammonium acetate 0.05 M -
pH 5.6
10.0 μl of Ammonium acetate (5 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
200.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
90.0 μl of water (100 v/v)
E6
  • Polyethylene glycol 400 30 v/v -
  • Polyethylene glycol 20,000 11 w/v -
  • di-Potassium hydrogen phosphate 0.1 M pH 8
pH 8
220.0 μl of Polyethylene glycol 20,000 (50 w/v)
300.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of di-Potassium hydrogen phosphate pH 8 (1 M)
380.0 μl of water (100 v/v)
E7
  • Polyethylene glycol 400 10 v/v -
  • Polyethylene glycol monomethyl ether 5,000 20 w/v -
NA
100.0 μl of Polyethylene glycol 400 (100 v/v)
400.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
500.0 μl of water (100 v/v)
E8
  • Polyethylene glycol 400 34 v/v -
  • HEPES 0.1 M pH 7.5
  • Sodium chloride 0.1 M -
  • Calcium chloride 0.2 M -
pH 7.5
40.0 μl of Calcium chloride (5 M)
100.0 μl of HEPES pH 7.5 (1 M)
340.0 μl of Polyethylene glycol 400 (100 v/v)
20.0 μl of Sodium chloride (5 M)
500.0 μl of water (100 v/v)
E9
  • Polyethylene glycol 400 40 v/v -
  • Polyethylene glycol 3,000 5 w/v -
  • MES 0.1 M pH 6.5
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
100.0 μl of Polyethylene glycol 3,000 (50 w/v)
400.0 μl of Polyethylene glycol 400 (100 v/v)
400.0 μl of water (100 v/v)
E10
  • Polyethylene glycol 600 35 v/v -
  • PIPES 0.1 M pH 7
  • Sodium chloride 0.1 M -
pH 7
100.0 μl of PIPES pH 7 (1 M)
700.0 μl of Polyethylene glycol 600 (50 v/v)
20.0 μl of Sodium chloride (5 M)
180.0 μl of water (100 v/v)
E11
  • Polyethylene glycol 1,000 30 w/v -
  • Sodium phosphate citrate 0.1 M pH 4.4
  • Lithium sulfate 0.11 M -
pH 4.4
36.7 μl of Lithium sulfate (3 M)
600.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of Sodium phosphate citrate pH 4.4 (1 M)
263.3 μl of water (100 v/v)
E12
  • Polyethylene glycol 1,500 40 w/v -
  • ADA 0.1 M pH 6
pH 6
100.0 μl of ADA pH 6 (1 M)
800.0 μl of Polyethylene glycol 1,500 (50 w/v)
100.0 μl of water (100 v/v)
F1
  • Polyethylene glycol 1,500 17 w/v -
  • 2-methyl-2,4-pentanediol BCC 10 v/v -
  • PIPES 0.5 M pH 7
pH 7
100.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
500.0 μl of PIPES pH 7 (1 M)
340.0 μl of Polyethylene glycol 1,500 (50 w/v)
60.0 μl of water (100 v/v)
F2
  • Polyethylene glycol 3,350 35 w/v -
  • Potassium thiocyanate 0.3 M -
NA
700.0 μl of Polyethylene glycol 3,350 (50 w/v)
37.5 μl of Potassium thiocyanate (8 M)
262.5 μl of water (100 v/v)
F3
  • Polyethylene glycol 4,000 25 w/v -
  • 2-methyl-2,4-pentanediol BCC 18 v/v -
  • Sodium acetate 0.1 M pH 4.6
  • Ammonium sulfate 0.2 M -
pH 4.6
180.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
66.7 μl of Ammonium sulfate (3 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
153.3 μl of water (100 v/v)
F4
  • Polyethylene glycol 4,000 25 w/v -
  • Ethylene glycol 30 v/v -
NA
300.0 μl of Ethylene glycol (100 v/v)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
200.0 μl of water (100 v/v)
F5
  • Polyethylene glycol 4,000 20 w/v -
  • 2-methyl-2,4-pentanediol BCC 20 v/v -
  • 2-Propanol 10 v/v -
  • tri-Sodium citrate 0.5 M pH 5.6
pH 5.6
100.0 μl of 2-Propanol (100 v/v)
200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
250.0 μl of tri-Sodium citrate pH 5.6 (2 M)
50.0 μl of water (100 v/v)
F6
  • Polyethylene glycol 4,000 10 w/v -
  • Ethylene glycol 20 v/v -
  • ADA 0.055 M pH 6
  • Sodium chloride 0.105 M -
pH 6
55.0 μl of ADA pH 6 (1 M)
200.0 μl of Ethylene glycol (100 v/v)
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
21.0 μl of Sodium chloride (5 M)
524.0 μl of water (100 v/v)
F7
  • Polyethylene glycol 400 2 v/v -
  • 2-methyl-2,4-pentanediol BCC 25 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
250.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
20.0 μl of Polyethylene glycol 400 (100 v/v)
630.0 μl of water (100 v/v)
F8
  • Polyethylene glycol 8,000 20 w/v -
  • 2-methyl-2,4-pentanediol BCC 20 v/v -
  • HEPES 0.1 M pH 7.5
  • Rubidium bromide 0.2 M -
pH 7.5
200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
200.0 μl of Rubidium bromide (1 M)
100.0 μl of water (100 v/v)
F9
  • Polyethylene glycol 8,000 28 w/v -
  • Glycine 0.1 M pH 9.5
  • Calcium chloride 0.01 M -
pH 9.5
2.0 μl of Calcium chloride (5 M)
50.0 μl of Glycine pH 9.5 (2 M)
560.0 μl of Polyethylene glycol 8,000 (50 w/v)
388.0 μl of water (100 v/v)
F10
  • Polyethylene glycol 8,000 10 w/v -
  • 2-methyl-2,4-pentanediol BCC 15 v/v -
  • MES 0.1 M pH 6.5
  • Ammonium sulfate 0.055 M -
pH 6.5
150.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
18.3 μl of Ammonium sulfate (3 M)
33.3 μl of MES pH 6.5 (3 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
598.3 μl of water (100 v/v)
F11
  • Polyethylene glycol 8,000 25 w/v -
  • Ethylene glycol 30 v/v -
  • ADA 0.15 M pH 6
  • Ammonium sulfate 0.08 M -
pH 6
150.0 μl of ADA pH 6 (1 M)
26.7 μl of Ammonium sulfate (3 M)
300.0 μl of Ethylene glycol (100 v/v)
500.0 μl of Polyethylene glycol 8,000 (50 w/v)
23.3 μl of water (100 v/v)
F12
  • Polyethylene glycol 20,000 10 w/v -
  • Ethylene glycol 5.6 v/v -
  • 2,3-Butanediol 30 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
300.0 μl of 2,3-Butanediol (100 v/v)
112.0 μl of Ethylene glycol (50 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 20,000 (50 w/v)
288.0 μl of water (100 v/v)
G1
  • Ethylene glycol 20 v/v -
  • HEPES 0.1 M pH 7.5
  • Lithium sulfate 1.6 M -
pH 7.5
200.0 μl of Ethylene glycol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
533.3 μl of Lithium sulfate (3 M)
166.7 μl of water (100 v/v)
G2
  • Ethylene glycol 25 v/v -
  • HEPES 0.1 M pH 7.5
  • Sodium Potassium phosphate 0.2 M -
pH 7.5
250.0 μl of Ethylene glycol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Sodium Potassium phosphate pH 7 (1 M)
450.0 μl of water (100 v/v)
G3
  • Ethylene glycol 25 v/v -
  • HEPES 0.1 M pH 7.5
  • Potassium Sodium tartrate 1 M -
pH 7.5
250.0 μl of Ethylene glycol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
500.0 μl of Potassium Sodium tartrate (2 M)
150.0 μl of water (100 v/v)
G4
  • Ethylene glycol 40 v/v -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
400.0 μl of Ethylene glycol (100 v/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
500.0 μl of water (100 v/v)
G5
  • Ethylene glycol 40 v/v -
  • tris chloride BCC 0.1 M pH 7.5
pH 7.5
400.0 μl of Ethylene glycol (100 v/v)
33.3 μl of tris chloride BCC pH 7.5 (3 M)
566.7 μl of water (100 v/v)
G6
  • Ethylene glycol 40 v/v -
  • Imidazole 0.1 M pH 9
  • Calcium acetate 0.2 M -
pH 9
100.0 μl of Calcium acetate (2 M)
400.0 μl of Ethylene glycol (100 v/v)
100.0 μl of Imidazole pH 9 (1 M)
400.0 μl of water (100 v/v)
G7
  • Ethylene glycol 40 v/v -
  • MES 0.1 M pH 6.5
  • Zinc acetate 0.2 M -
pH 6.5
400.0 μl of Ethylene glycol (100 v/v)
33.3 μl of MES pH 6.5 (3 M)
100.0 μl of Zinc acetate (2 M)
466.7 μl of water (100 v/v)
G8
  • Ethylene glycol 50 v/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Magnesium chloride 0.2 M -
pH 8.5
500.0 μl of Ethylene glycol (100 v/v)
80.0 μl of Magnesium chloride pH 7 (2.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
320.0 μl of water (100 v/v)
G9
  • Ethylene glycol 50 v/v -
  • HEPES 0.1 M pH 7.5
  • Lithium sulfate 0.2 M -
pH 7.5
500.0 μl of Ethylene glycol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
66.7 μl of Lithium sulfate (3 M)
333.3 μl of water (100 v/v)
G10
  • 2-Propanol 35 v/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
350.0 μl of 2-Propanol (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
550.0 μl of water (100 v/v)
G11
  • 2-Propanol 35 v/v -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
350.0 μl of 2-Propanol (100 v/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
550.0 μl of water (100 v/v)
G12
  • 2-Propanol 35 v/v -
  • Imidazole 0.1 M pH 9
  • Zinc acetate 0.2 M -
pH 9
350.0 μl of 2-Propanol (100 v/v)
100.0 μl of Imidazole pH 9 (1 M)
100.0 μl of Zinc acetate (2 M)
450.0 μl of water (100 v/v)
H1
  • 1,2-Propanediol 40 v/v -
  • Imidazole 0.1 M pH 9
pH 9
400.0 μl of 1,2-Propanediol (100 v/v)
100.0 μl of Imidazole pH 9 (1 M)
500.0 μl of water (100 v/v)
H2
  • 1,2-Propanediol 40 v/v -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
400.0 μl of 1,2-Propanediol (100 v/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
500.0 μl of water (100 v/v)
H3
  • 1,2-Propanediol 40 v/v -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Sodium chloride 0.2 M -
pH 5.6
400.0 μl of 1,2-Propanediol (100 v/v)
200.0 μl of Sodium chloride (1 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
300.0 μl of water (100 v/v)
H4
  • 1,2-Propanediol 40 v/v -
  • Sodium acetate 0.1 M pH 4.6
  • Calcium acetate 0.5 M -
pH 4.6
400.0 μl of 1,2-Propanediol (100 v/v)
250.0 μl of Calcium acetate (2 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
250.0 μl of water (100 v/v)
H5
  • 2-methyl-2,4-pentanediol BCC 35 v/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
550.0 μl of water (100 v/v)
H6
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
  • Sodium acetate 0.1 M pH 4.6
  • Calcium chloride 0.2 M -
pH 4.6
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
40.0 μl of Calcium chloride (5 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
560.0 μl of water (100 v/v)
H7
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
  • HEPES 0.1 M pH 7.5
  • tri-Sodium citrate 0.2 M -
pH 7.5
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
40.0 μl of tri-Sodium citrate (5 M)
560.0 μl of water (100 v/v)
H8
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Ammonium acetate 0.2 M -
pH 5.6
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
40.0 μl of Ammonium acetate (5 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
560.0 μl of water (100 v/v)
H9
  • 2-methyl-2,4-pentanediol BCC 35 v/v -
  • MES 0.1 M pH 6.5
  • Ammonium sulfate 0.2 M -
pH 6.5
350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
66.7 μl of Ammonium sulfate (3 M)
33.3 μl of MES pH 6.5 (3 M)
550.0 μl of water (100 v/v)
H10
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
  • tri-Sodium citrate 0.1 M pH 5.6
pH 5.6
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
500.0 μl of water (100 v/v)
H11
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
  • MES 0.1 M pH 6.5
  • Ammonium sulfate 0.2 M -
pH 6.5
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
66.7 μl of Ammonium sulfate (3 M)
33.3 μl of MES pH 6.5 (3 M)
500.0 μl of water (100 v/v)
H12
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
  • Imidazole 0.1 M pH 9
  • Magnesium chloride 0.2 M -
pH 9
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Imidazole pH 9 (1 M)
80.0 μl of Magnesium chloride pH 7 (2.5 M)
420.0 μl of water (100 v/v)