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Supplemental Information

Screen Description

Salt Rx HT Screen

Hampton Research / Salt Rx HT Screen
Created 2009-11-16.

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Salt Rx HT Screen

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Salt Rx HT Screen

    Screen Structure

    Salt Rx HT Screen

    Well Count 96
    Number of distinct ingredients 28
    Internal diversity (score mean dist) 0.884

    Screen Composition

    More information about screen composition chart.
    2025-06-14T13:35:38.192678 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Salt Rx HT Screen
    Buffer 144
    Complex ingredient 2
    Salt 127

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Salt Rx HT Screen Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Sodium acetate 1.8 M -
    • BIS-TRIS propane 0.1 M pH 7
    		pH 7
    100 μl of BIS-TRIS propane pH 7 (1 M)
    450 μl of Sodium acetate (4 M)
    450 μl of water (100 v/v)
    A2
    • Sodium acetate 2.8 M -
    • BIS-TRIS propane 0.1 M pH 7
    		pH 7
    100 μl of BIS-TRIS propane pH 7 (1 M)
    700 μl of Sodium acetate (4 M)
    200 μl of water (100 v/v)
    A3
    • Ammonium chloride 1.5 M -
    • Sodium acetate 0.1 M pH 4.6
    		pH 4.6
    300 μl of Ammonium chloride (5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    600 μl of water (100 v/v)
    A4
    • Ammonium chloride 1.5 M -
    • BIS-TRIS propane 0.1 M pH 7
    		pH 7
    300 μl of Ammonium chloride (5 M)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    600 μl of water (100 v/v)
    A5
    • Ammonium chloride 1.5 M -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    300 μl of Ammonium chloride (5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    600 μl of water (100 v/v)
    A6
    • Ammonium chloride 3.5 M -
    • Sodium acetate 0.1 M pH 4.6
    		pH 4.6
    700 μl of Ammonium chloride (5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    200 μl of water (100 v/v)
    A7
    • Ammonium chloride 3.5 M -
    • BIS-TRIS propane 0.1 M pH 7
    		pH 7
    700 μl of Ammonium chloride (5 M)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    200 μl of water (100 v/v)
    A8
    • Ammonium chloride 3.5 M -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    700 μl of Ammonium chloride (5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    200 μl of water (100 v/v)
    A9
    • Sodium chloride 2.2 M -
    • Sodium acetate 0.1 M pH 4.6
    		pH 4.6
    440 μl of Sodium chloride (5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    460 μl of water (100 v/v)
    A10
    • Sodium chloride 2.2 M -
    • BIS-TRIS propane 0.1 M pH 7
    		pH 7
    440 μl of Sodium chloride (5 M)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    460 μl of water (100 v/v)
    A11
    • Sodium chloride 2.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    440 μl of Sodium chloride (5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    460 μl of water (100 v/v)
    A12
    • Sodium chloride 3.2 M -
    • Sodium acetate 0.1 M pH 4.6
    		pH 4.6
    640 μl of Sodium chloride (5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    260 μl of water (100 v/v)
    B1
    • Sodium chloride 3.2 M -
    • BIS-TRIS propane 0.1 M pH 7
    		pH 7
    640 μl of Sodium chloride (5 M)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    260 μl of water (100 v/v)
    B2
    • Sodium chloride 3.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    640 μl of Sodium chloride (5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    260 μl of water (100 v/v)
    B3
    • Sodium acetate 0.1 M pH 4.6
    • di-Ammonium hydrogen citrate 1 M -
    		pH 4.6
    400 μl of di-Ammonium hydrogen citrate (2.5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    500 μl of water (100 v/v)
    B4
    • Sodium acetate 0.1 M pH 4.6
    • di-Ammonium hydrogen citrate 1.8 M -
    		pH 4.6
    720 μl of di-Ammonium hydrogen citrate (2.5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    180 μl of water (100 v/v)
    B5
    • BIS-TRIS propane 0.1 M pH 7
    • tri-Ammonium citrate 1 M -
    		pH 7
    400 μl of tri-Ammonium citrate (2.5 M)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    500 μl of water (100 v/v)
    B6
    • BIS-TRIS propane 0.1 M pH 7
    • di-Ammonium hydrogen citrate 2 M -
    		pH 7
    800 μl of di-Ammonium hydrogen citrate (2.5 M)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    100 μl of water (100 v/v)
    B7
    • BIS-TRIS propane 0.1 M pH 7
    • tri-Sodium citrate 0.7 M -
    		pH 7
    437 μl of tri-Sodium citrate (1.6 M)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    462 μl of water (100 v/v)
    B8
    • tris chloride BCC 0.1 M pH 8.5
    • tri-Sodium citrate 0.7 M -
    		pH 8.5
    437 μl of tri-Sodium citrate (1.6 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    462 μl of water (100 v/v)
    B9
    • BIS-TRIS propane 0.1 M pH 7
    • tri-Sodium citrate 1.2 M -
    		pH 7
    750 μl of tri-Sodium citrate (1.6 M)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    150 μl of water (100 v/v)
    B10
    • tris chloride BCC 0.1 M pH 8.5
    • tri-Sodium citrate 1.2 M -
    		pH 8.5
    750 μl of tri-Sodium citrate (1.6 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    150 μl of water (100 v/v)
    B11
    • Sodium acetate 0.1 M pH 4.6
    • Magnesium formate 0.4 M -
    		pH 4.6
    400 μl of Magnesium formate (1 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    500 μl of water (100 v/v)
    B12
    • BIS-TRIS propane 0.1 M pH 7
    • Magnesium formate 0.4 M -
    		pH 7
    400 μl of Magnesium formate (1 M)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    500 μl of water (100 v/v)
    C1
    • tris chloride BCC 0.1 M pH 8.5
    • Magnesium formate 0.4 M -
    		pH 8.5
    400 μl of Magnesium formate (1 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    500 μl of water (100 v/v)
    C2
    • BIS-TRIS propane 0.1 M pH 7
    • Magnesium formate 0.7 M -
    		pH 7
    700 μl of Magnesium formate (1 M)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    200 μl of water (100 v/v)
    C3
    • Sodium acetate 0.1 M pH 4.6
    • Sodium formate 2 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    285 μl of Sodium formate (7 M)
    614 μl of water (100 v/v)
    C4
    • BIS-TRIS propane 0.1 M pH 7
    • Sodium formate 2 M -
    		pH 7
    100 μl of BIS-TRIS propane pH 7 (1 M)
    285 μl of Sodium formate (7 M)
    614 μl of water (100 v/v)
    C5
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium formate 2 M -
    		pH 8.5
    285 μl of Sodium formate (7 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    614 μl of water (100 v/v)
    C6
    • Sodium acetate 0.1 M pH 4.6
    • Sodium formate 3.5 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    500 μl of Sodium formate (7 M)
    400 μl of water (100 v/v)
    C7
    • BIS-TRIS propane 0.1 M pH 7
    • Sodium formate 3.5 M -
    		pH 7
    100 μl of BIS-TRIS propane pH 7 (1 M)
    500 μl of Sodium formate (7 M)
    400 μl of water (100 v/v)
    C8
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium formate 3.5 M -
    		pH 8.5
    500 μl of Sodium formate (7 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of water (100 v/v)
    C9
    • BIS-TRIS propane 0.1 M pH 7
    • di-Sodium DL-malate 1.2 M -
    		pH 7
    400 μl of di-Sodium DL-malate (3 M)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    500 μl of water (100 v/v)
    C10
    • BIS-TRIS propane 0.1 M pH 7
    • di-Sodium DL-malate 2.2 M -
    		pH 7
    733 μl of di-Sodium DL-malate (3 M)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    166 μl of water (100 v/v)
    C11
    • BIS-TRIS propane 0.1 M pH 7
    • di-Sodium malonate 1.4 M -
    		pH 7
    411 μl of di-Sodium malonate pH 4 (3.4 M)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    488 μl of water (100 v/v)
    C12
    • BIS-TRIS propane 0.1 M pH 7
    • di-Sodium malonate 2.4 M -
    		pH 7
    705 μl of di-Sodium malonate pH 4 (3.4 M)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    194 μl of water (100 v/v)
    D1
    • Sodium acetate 0.1 M pH 4.6
    • Ammonium nitrate 2.5 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    250 μl of Ammonium nitrate (10 M)
    650 μl of water (100 v/v)
    D2
    • BIS-TRIS propane 0.1 M pH 7
    • Ammonium nitrate 2.5 M -
    		pH 7
    100 μl of BIS-TRIS propane pH 7 (1 M)
    250 μl of Ammonium nitrate (10 M)
    650 μl of water (100 v/v)
    D3
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium nitrate 2.5 M -
    		pH 8.5
    250 μl of Ammonium nitrate (10 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    650 μl of water (100 v/v)
    D4
    • Sodium acetate 0.1 M pH 4.6
    • Ammonium nitrate 6 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    600 μl of Ammonium nitrate (10 M)
    300 μl of water (100 v/v)
    D5
    • BIS-TRIS propane 0.1 M pH 7
    • Ammonium nitrate 6 M -
    		pH 7
    100 μl of BIS-TRIS propane pH 7 (1 M)
    600 μl of Ammonium nitrate (10 M)
    300 μl of water (100 v/v)
    D6
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium nitrate 6 M -
    		pH 8.5
    600 μl of Ammonium nitrate (10 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    300 μl of water (100 v/v)
    D7
    • Sodium acetate 0.1 M pH 4.6
    • Sodium nitrate 1.5 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    214 μl of Sodium nitrate (7 M)
    685 μl of water (100 v/v)
    D8
    • BIS-TRIS propane 0.1 M pH 7
    • Sodium nitrate 1.5 M -
    		pH 7
    100 μl of BIS-TRIS propane pH 7 (1 M)
    214 μl of Sodium nitrate (7 M)
    685 μl of water (100 v/v)
    D9
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium nitrate 1.5 M -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    214 μl of Sodium nitrate (7 M)
    685 μl of water (100 v/v)
    D10
    • Sodium acetate 0.1 M pH 4.6
    • Sodium nitrate 4 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    571 μl of Sodium nitrate (7 M)
    328 μl of water (100 v/v)
    D11
    • BIS-TRIS propane 0.1 M pH 7
    • Sodium nitrate 4 M -
    		pH 7
    100 μl of BIS-TRIS propane pH 7 (1 M)
    571 μl of Sodium nitrate (7 M)
    328 μl of water (100 v/v)
    D12
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium nitrate 4 M -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    571 μl of Sodium nitrate (7 M)
    328 μl of water (100 v/v)
    E1
    • Sodium acetate 0.1 M pH 4.6
    • Ammonium di-hydrogen phosphate 1 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    400 μl of Ammonium di-hydrogen phosphate (2.5 M)
    500 μl of water (100 v/v)
    E2
    • Sodium acetate 0.1 M pH 4.6
    • Ammonium di-hydrogen phosphate 1.8 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    720 μl of Ammonium di-hydrogen phosphate (2.5 M)
    180 μl of water (100 v/v)
    E3
    • tris chloride BCC 0.1 M pH 8.5
    • di-Ammonium hydrogen phosphate 1.5 M -
    		pH 8.5
    428 μl of di-Ammonium hydrogen phosphate (3.5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    471 μl of water (100 v/v)
    E4
    • tris chloride BCC 0.1 M pH 8.5
    • di-Ammonium hydrogen phosphate 2.4 M -
    		pH 8.5
    685 μl of di-Ammonium hydrogen phosphate (3.5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    214 μl of water (100 v/v)
    E5
    • Sodium di-hydrogen phosphate 0.98 M -
    • di-Potassium hydrogen phosphate 0.02 M -
    		NA
    245 μl of Sodium di-hydrogen phosphate (4 M)
    5 μl of di-Potassium hydrogen phosphate (4 M)
    750 μl of water (100 v/v)
    E6
    • Sodium di-hydrogen phosphate 0.35 M -
    • di-Potassium hydrogen phosphate 0.65 M -
    		NA
    87 μl of Sodium di-hydrogen phosphate (4 M)
    162 μl of di-Potassium hydrogen phosphate (4 M)
    750 μl of water (100 v/v)
    E7
    • Sodium di-hydrogen phosphate 0.04 M -
    • di-Potassium hydrogen phosphate 0.96 M -
    		NA
    10 μl of Sodium di-hydrogen phosphate (4 M)
    240 μl of di-Potassium hydrogen phosphate (4 M)
    750 μl of water (100 v/v)
    E8
    • Sodium di-hydrogen phosphate 1.76 M -
    • di-Potassium hydrogen phosphate 0.036 M -
    		NA
    441 μl of Sodium di-hydrogen phosphate (4 M)
    9 μl of di-Potassium hydrogen phosphate (4 M)
    550 μl of water (100 v/v)
    E9
    • Sodium di-hydrogen phosphate 0.63 M -
    • di-Potassium hydrogen phosphate 1.17 M -
    		NA
    157 μl of Sodium di-hydrogen phosphate (4 M)
    292 μl of di-Potassium hydrogen phosphate (4 M)
    550 μl of water (100 v/v)
    E10
    • Sodium di-hydrogen phosphate 0.072 M -
    • di-Potassium hydrogen phosphate 1.73 M -
    		NA
    18 μl of Sodium di-hydrogen phosphate (4 M)
    432 μl of di-Potassium hydrogen phosphate (4 M)
    550 μl of water (100 v/v)
    E11
    • BIS-TRIS propane 0.1 M pH 7
    • di-Sodium succinate 0.5 M -
    		pH 7
    416 μl of di-Sodium succinate (1.2 M)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    483 μl of water (100 v/v)
    E12
    • BIS-TRIS propane 0.1 M pH 7
    • di-Sodium succinate 1 M -
    		pH 7
    833 μl of di-Sodium succinate (1.2 M)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    66 μl of water (100 v/v)
    F1
    • Sodium acetate 0.1 M pH 4.6
    • Ammonium sulfate 1.5 M -
    		pH 4.6
    428 μl of Ammonium sulfate (3.5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    471 μl of water (100 v/v)
    F2
    • BIS-TRIS propane 0.1 M pH 7
    • Ammonium sulfate 1.5 M -
    		pH 7
    428 μl of Ammonium sulfate (3.5 M)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    471 μl of water (100 v/v)
    F3
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium sulfate 1.5 M -
    		pH 8.5
    428 μl of Ammonium sulfate (3.5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    471 μl of water (100 v/v)
    F4
    • Sodium acetate 0.1 M pH 4.6
    • Ammonium sulfate 2.5 M -
    		pH 4.6
    714 μl of Ammonium sulfate (3.5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    185 μl of water (100 v/v)
    F5
    • BIS-TRIS propane 0.1 M pH 7
    • Ammonium sulfate 2.5 M -
    		pH 7
    714 μl of Ammonium sulfate (3.5 M)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    185 μl of water (100 v/v)
    F6
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium sulfate 2.5 M -
    		pH 8.5
    714 μl of Ammonium sulfate (3.5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    185 μl of water (100 v/v)
    F7
    • Sodium acetate 0.1 M pH 4.6
    • Lithium sulfate 0.8 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    400 μl of Lithium sulfate (2 M)
    500 μl of water (100 v/v)
    F8
    • BIS-TRIS propane 0.1 M pH 7
    • Lithium sulfate 0.8 M -
    		pH 7
    400 μl of Lithium sulfate (2 M)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    500 μl of water (100 v/v)
    F9
    • tris chloride BCC 0.1 M pH 8.5
    • Lithium sulfate 0.8 M -
    		pH 8.5
    400 μl of Lithium sulfate (2 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    500 μl of water (100 v/v)
    F10
    • Sodium acetate 0.1 M pH 4.6
    • Lithium sulfate 1.5 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    750 μl of Lithium sulfate (2 M)
    150 μl of water (100 v/v)
    F11
    • BIS-TRIS propane 0.1 M pH 7
    • Lithium sulfate 1.5 M -
    		pH 7
    750 μl of Lithium sulfate (2 M)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    150 μl of water (100 v/v)
    F12
    • tris chloride BCC 0.1 M pH 8.5
    • Lithium sulfate 1.5 M -
    		pH 8.5
    750 μl of Lithium sulfate (2 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    150 μl of water (100 v/v)
    G1
    • Sodium acetate 0.1 M pH 4.6
    • Magnesium sulfate 1 M -
    		pH 4.6
    400 μl of Magnesium sulfate (2.5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    500 μl of water (100 v/v)
    G2
    • BIS-TRIS propane 0.1 M pH 7
    • Magnesium sulfate 1 M -
    		pH 7
    400 μl of Magnesium sulfate (2.5 M)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    500 μl of water (100 v/v)
    G3
    • tris chloride BCC 0.1 M pH 8.5
    • Magnesium sulfate 1 M -
    		pH 8.5
    400 μl of Magnesium sulfate (2.5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    500 μl of water (100 v/v)
    G4
    • Sodium acetate 0.1 M pH 4.6
    • Magnesium sulfate 1.8 M -
    		pH 4.6
    720 μl of Magnesium sulfate (2.5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    180 μl of water (100 v/v)
    G5
    • BIS-TRIS propane 0.1 M pH 7
    • Magnesium sulfate 1.8 M -
    		pH 7
    720 μl of Magnesium sulfate (2.5 M)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    180 μl of water (100 v/v)
    G6
    • tris chloride BCC 0.1 M pH 8.5
    • Magnesium sulfate 1.8 M -
    		pH 8.5
    720 μl of Magnesium sulfate (2.5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    180 μl of water (100 v/v)
    G7
    • Sodium acetate 0.1 M pH 4.6
    • di-Ammonium tartrate 0.7 M -
    		pH 4.6
    350 μl of di-Ammonium tartrate (2 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    550 μl of water (100 v/v)
    G8
    • BIS-TRIS propane 0.1 M pH 7
    • di-Ammonium tartrate 0.7 M -
    		pH 7
    350 μl of di-Ammonium tartrate (2 M)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    550 μl of water (100 v/v)
    G9
    • tris chloride BCC 0.1 M pH 8.5
    • di-Ammonium tartrate 0.7 M -
    		pH 8.5
    350 μl of di-Ammonium tartrate (2 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    550 μl of water (100 v/v)
    G10
    • Sodium acetate 0.1 M pH 4.6
    • di-Ammonium tartrate 1 M -
    		pH 4.6
    500 μl of di-Ammonium tartrate (2 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    400 μl of water (100 v/v)
    G11
    • BIS-TRIS propane 0.1 M pH 7
    • di-Ammonium tartrate 1.3 M -
    		pH 7
    650 μl of di-Ammonium tartrate (2 M)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    250 μl of water (100 v/v)
    G12
    • tris chloride BCC 0.1 M pH 8.5
    • di-Ammonium tartrate 1.4 M -
    		pH 8.5
    700 μl of di-Ammonium tartrate (2 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    200 μl of water (100 v/v)
    H1
    • BIS-TRIS propane 0.1 M pH 7
    • Potassium Sodium tartrate 0.6 M -
    		pH 7
    100 μl of BIS-TRIS propane pH 7 (1 M)
    400 μl of Potassium Sodium tartrate (1.5 M)
    500 μl of water (100 v/v)
    H2
    • BIS-TRIS propane 0.1 M pH 7
    • Potassium Sodium tartrate 1.2 M -
    		pH 7
    100 μl of BIS-TRIS propane pH 7 (1 M)
    800 μl of Potassium Sodium tartrate (1.5 M)
    100 μl of water (100 v/v)
    H3
    • tris chloride BCC 0.1 M pH 8.5
    • Potassium Sodium tartrate 0.6 M -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Potassium Sodium tartrate (1.5 M)
    500 μl of water (100 v/v)
    H4
    • tris chloride BCC 0.1 M pH 8.5
    • Potassium Sodium tartrate 1.2 M -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    800 μl of Potassium Sodium tartrate (1.5 M)
    100 μl of water (100 v/v)
    H5
    • Sodium acetate 0.1 M pH 4.6
    • Potassium thiocyanate 0.5 M -
    		pH 4.6
    62 μl of Potassium thiocyanate (8 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    837 μl of water (100 v/v)
    H6
    • BIS-TRIS propane 0.1 M pH 7
    • Potassium thiocyanate 0.5 M -
    		pH 7
    62 μl of Potassium thiocyanate (8 M)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    837 μl of water (100 v/v)
    H7
    • tris chloride BCC 0.1 M pH 8.5
    • Potassium thiocyanate 0.5 M -
    		pH 8.5
    62 μl of Potassium thiocyanate (8 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    837 μl of water (100 v/v)
    H8
    • Sodium acetate 0.1 M pH 4.6
    • Ammonium acetate 4 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    500 μl of Ammonium acetate (8 M)
    400 μl of water (100 v/v)
    H9
    • BIS-TRIS propane 0.1 M pH 7
    • Ammonium acetate 4 M -
    		pH 7
    100 μl of BIS-TRIS propane pH 7 (1 M)
    500 μl of Ammonium acetate (8 M)
    400 μl of water (100 v/v)
    H10
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium acetate 4 M -
    		pH 8.5
    500 μl of Ammonium acetate (8 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of water (100 v/v)
    H11
    • BIS-TRIS propane 0.1 M pH 7
    • Tacsimate 35 v/v -
    		pH 7
    350 μl of Tacsimate pH 7 (100 v/v)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    550 μl of water (100 v/v)
    H12
    • BIS-TRIS propane 0.1 M pH 7
    • Tacsimate 60 v/v -
    		pH 7
    600 μl of Tacsimate pH 7 (100 v/v)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    300 μl of water (100 v/v)