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Supplemental Information

Screen Description

Phosphatase HTS

Phosphatase / Phosphatase HTS
Created 2009-11-16.

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Phosphatase HTS

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Screen Structure

Phosphatase HTS

Well Count 96
Number of distinct ingredients 68
Internal diversity (score mean dist) 0.946

Screen Composition

2025-08-16T09:05:42.402864 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Phosphatase HTS
Buffer 116
Cryoprotectant 5
Detergent 1
Organic (non-volatile) 20
Organic (volatile) 6
Polymer 79
Salt 114

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Phosphatase HTS Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Polyethylene glycol 400 15 v/v -
  • tri-Sodium citrate 0.05 M pH 5.6
  • Ammonium sulfate 0.2 M -
  • di-Sodium tartrate 0.2 M -
pH 5.6
66.7 μl of Ammonium sulfate (3 M)
150.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of di-Sodium tartrate (2 M)
25.0 μl of tri-Sodium citrate pH 5.6 (2 M)
658.3 μl of water (100 v/v)
A2
  • Polyethylene glycol 400 22 v/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Lithium sulfate 0.2 M -
pH 8.5
66.7 μl of Lithium sulfate (3 M)
220.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
613.3 μl of water (100 v/v)
A3
  • Polyethylene glycol 400 25 v/v -
  • HEPES 0.1 M pH 7.5
  • Cesium chloride 0.2 M -
pH 7.5
200.0 μl of Cesium chloride (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
250.0 μl of Polyethylene glycol 400 (100 v/v)
450.0 μl of water (100 v/v)
A4
  • Polyethylene glycol monomethyl ether 550 25 v/v -
  • MES 0.1 M pH 6.5
  • Zinc sulfate 0.01 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
250.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
10.0 μl of Zinc sulfate (1 M)
640.0 μl of water (100 v/v)
A5
  • Polyethylene glycol 1,500 27 w/v -
  • 2-methyl-2,4-pentanediol BCC 10 v/v -
  • Imidazole 0.01 M pH 7
  • Sodium nitrate 0.22 M -
pH 7
100.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
10.0 μl of Imidazole pH 7 (1 M)
540.0 μl of Polyethylene glycol 1,500 (50 w/v)
31.4 μl of Sodium nitrate (7 M)
318.6 μl of water (100 v/v)
A6
  • Polyethylene glycol 1,500 30 w/v -
  • BIS-TRIS propane 0.1 M pH 6.5
  • Potassium fluoride 0.4 M -
pH 6.5
100.0 μl of BIS-TRIS propane pH 6.5 (1 M)
600.0 μl of Polyethylene glycol 1,500 (50 w/v)
66.7 μl of Potassium fluoride (6 M)
233.3 μl of water (100 v/v)
A7
  • Polyethylene glycol monomethyl ether 2,000 8 w/v -
  • tris chloride BCC 0.01 M pH 7
  • Nickel (II) chloride 0.01 M -
pH 7
10.0 μl of Nickel (II) chloride pH 7 (1 M)
160.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
3.3 μl of tris chloride BCC pH 7 (3 M)
826.7 μl of water (100 v/v)
A8
  • Polyethylene glycol monomethyl ether 2,000 16 w/v -
  • Sodium acetate 0.1 M pH 4.6
  • Ammonium sulfate 0.4 M -
pH 4.6
133.3 μl of Ammonium sulfate (3 M)
320.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
446.7 μl of water (100 v/v)
A9
  • Polyethylene glycol 3,350 8 w/v -
  • Sodium acetate 0.1 M -
NA
160.0 μl of Polyethylene glycol 3,350 (50 w/v)
50.0 μl of Sodium acetate (2 M)
790.0 μl of water (100 v/v)
A10
  • Polyethylene glycol 3,350 10 w/v -
  • HEPES 0.1 M pH 7.5
  • Ammonium acetate 0.2 M -
pH 7.5
40.0 μl of Ammonium acetate (5 M)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 3,350 (50 w/v)
660.0 μl of water (100 v/v)
A11
  • Polyethylene glycol 3,350 10 w/v -
  • HEPES 0.1 M pH 7.5
  • Ammonium acetate 0.05 M -
  • Sodium chloride 0.015 M -
pH 7.5
10.0 μl of Ammonium acetate (5 M)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 3,350 (50 w/v)
15.0 μl of Sodium chloride (1 M)
675.0 μl of water (100 v/v)
A12
  • Polyethylene glycol 3,350 10 w/v -
  • MES 0.1 M pH 6.5
  • Zinc acetate 0.02 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
200.0 μl of Polyethylene glycol 3,350 (50 w/v)
10.0 μl of Zinc acetate (2 M)
690.0 μl of water (100 v/v)
B1
  • Polyethylene glycol 3,350 15 w/v -
  • Sodium fluoride 0.2 M -
NA
300.0 μl of Polyethylene glycol 3,350 (50 w/v)
250.0 μl of Sodium fluoride (0.8 M)
450.0 μl of water (100 v/v)
B2
  • Polyethylene glycol 3,350 15 w/v -
  • BIS-TRIS propane 0.1 M pH 6.5
  • Glycine 0.05 M -
  • Sodium formate 0.2 M -
pH 6.5
100.0 μl of BIS-TRIS propane pH 6.5 (1 M)
25.0 μl of Glycine (2 M)
300.0 μl of Polyethylene glycol 3,350 (50 w/v)
28.6 μl of Sodium formate (7 M)
546.4 μl of water (100 v/v)
B3
  • Polyethylene glycol 3,350 15 w/v -
  • Glycerol 5 v/v -
  • BIS-TRIS propane 0.1 M pH 7
  • Magnesium formate 0.2 M -
pH 7
100.0 μl of BIS-TRIS propane pH 7 (1 M)
50.0 μl of Glycerol (100 v/v)
200.0 μl of Magnesium formate (1 M)
300.0 μl of Polyethylene glycol 3,350 (50 w/v)
350.0 μl of water (100 v/v)
B4
  • Polyethylene glycol 3,350 20 w/v -
  • Potassium fluoride 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
33.3 μl of Potassium fluoride (6 M)
566.7 μl of water (100 v/v)
B5
  • Polyethylene glycol 3,350 20 w/v -
  • MES 0.1 M pH 6.5
  • Ammonium chloride 0.1 M -
pH 6.5
20.0 μl of Ammonium chloride (5 M)
100.0 μl of MES pH 6.5 (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
480.0 μl of water (100 v/v)
B6
  • Polyethylene glycol 3,350 20 w/v -
  • tris chloride BCC 0.01 M pH 8
  • Potassium sulfate 0.2 M -
pH 8
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of Potassium sulfate (0.5 M)
10.0 μl of tris chloride BCC pH 8 (1 M)
190.0 μl of water (100 v/v)
B7
  • Polyethylene glycol 3,350 20 w/v -
  • tri-Sodium citrate 0.1 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
50.0 μl of tri-Sodium citrate (2 M)
550.0 μl of water (100 v/v)
B8
  • Polyethylene glycol 3,350 22 w/v -
  • HEPES 0.1 M pH 7.5
  • Calcium chloride 0.2 M -
pH 7.5
40.0 μl of Calcium chloride (5 M)
100.0 μl of HEPES pH 7.5 (1 M)
440.0 μl of Polyethylene glycol 3,350 (50 w/v)
420.0 μl of water (100 v/v)
B9
  • Polyethylene glycol 3,350 25 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Magnesium chloride 0.2 M -
pH 8.5
80.0 μl of Magnesium chloride pH 7 (2.5 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
320.0 μl of water (100 v/v)
B10
  • Polyethylene glycol 3,350 25 w/v -
  • 2-Propanol 10 v/v -
  • tri-Sodium citrate 0.1 M pH 4.8
  • Lithium chloride 0.1 M -
pH 4.8
100.0 μl of 2-Propanol (100 v/v)
20.0 μl of Lithium chloride (5 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tri-Sodium citrate pH 4.8 (1 M)
280.0 μl of water (100 v/v)
B11
  • Polyethylene glycol 3,350 30 w/v -
  • Ammonium nitrate 0.2 M -
NA
20.0 μl of Ammonium nitrate (10 M)
600.0 μl of Polyethylene glycol 3,350 (50 w/v)
380.0 μl of water (100 v/v)
B12
  • Polyethylene glycol 3,350 30 w/v -
  • Ammonium di-hydrogen phosphate 0.2 M -
NA
66.7 μl of Ammonium di-hydrogen phosphate (3 M)
600.0 μl of Polyethylene glycol 3,350 (50 w/v)
333.3 μl of water (100 v/v)
C1
  • Polyethylene glycol 4,000 8 w/v -
  • HEPES 0.05 M pH 7
  • Lithium sulfate 0.022 M -
  • ß-Mercaptoethanol 0.1 v/v -
pH 7
50.0 μl of HEPES pH 7 (1 M)
7.3 μl of Lithium sulfate (3 M)
160.0 μl of Polyethylene glycol 4,000 (50 w/v)
50.0 μl of ß-Mercaptoethanol (2 v/v)
732.7 μl of water (100 v/v)
C2
  • Polyethylene glycol 4,000 10 w/v -
  • HEPES 0.1 M pH 7.5
  • Ammonium acetate 0.05 M -
pH 7.5
10.0 μl of Ammonium acetate (5 M)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
690.0 μl of water (100 v/v)
C3
  • Polyethylene glycol 4,000 10 w/v -
  • Glycerol 10 v/v -
  • di-Sodium succinate 0.05 M pH 5.5
  • Magnesium chloride 0.003 M -
  • TRIS-Phosphate 0.05 M -
pH 5.5
100.0 μl of Glycerol (100 v/v)
1.2 μl of Magnesium chloride pH 7 (2.5 M)
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
25.0 μl of TRIS-Phosphate (2 M)
16.7 μl of di-Sodium succinate pH 5.5 (3 M)
657.1 μl of water (100 v/v)
C4
  • Polyethylene glycol 4,000 12 w/v -
  • tri-Sodium citrate 0.1 M pH 5.6
  • 2-Propanol 10 v/v -
pH 5.6
100.0 μl of 2-Propanol (100 v/v)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
25.0 μl of tri-Sodium citrate pH 5.6 (4 M)
635.0 μl of water (100 v/v)
C5
  • Polyethylene glycol 4,000 12 w/v -
  • Imidazole-Malate 0.2 M pH 6
  • Zinc acetate 0.002 M -
pH 6
100.0 μl of Imidazole-Malate pH 6 (2 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
1.0 μl of Zinc acetate (2 M)
659.0 μl of water (100 v/v)
C6
  • Polyethylene glycol 4,000 15 w/v -
  • HEPES 0.1 M pH 7
  • Magnesium chloride 0.2 M -
pH 7
100.0 μl of HEPES pH 7 (1 M)
80.0 μl of Magnesium chloride pH 7 (2.5 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
520.0 μl of water (100 v/v)
C7
  • Polyethylene glycol 4,000 15 w/v -
  • Polyethylene glycol 400 15 v/v -
  • MES 0.05 M pH 6.5
  • Magnesium sulfate 0.2 M -
  • Ammonium sulfate 0.05 M -
pH 6.5
16.7 μl of Ammonium sulfate (3 M)
50.0 μl of MES pH 6.5 (1 M)
200.0 μl of Magnesium sulfate (1 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
150.0 μl of Polyethylene glycol 400 (100 v/v)
283.3 μl of water (100 v/v)
C8
  • Polyethylene glycol 4,000 15 w/v -
  • MES 0.085 M pH 6.5
  • Sodium acetate 0.17 M -
pH 6.5
85.0 μl of MES pH 6.5 (1 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
85.0 μl of Sodium acetate (2 M)
530.0 μl of water (100 v/v)
C9
  • Polyethylene glycol 4,000 20 w/v -
  • BICINE 0.1 M pH 9
  • 2-Propanol 5 v/v -
pH 9
50.0 μl of 2-Propanol (100 v/v)
100.0 μl of BICINE pH 9 (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
450.0 μl of water (100 v/v)
C10
  • Polyethylene glycol 4,000 22 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Lithium sulfate 0.2 M -
  • 2-Propanol 10 v/v -
  • ß-Mercaptoethanol 0.5 v/v -
pH 8.5
100.0 μl of 2-Propanol (100 v/v)
66.7 μl of Lithium sulfate (3 M)
440.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
250.0 μl of ß-Mercaptoethanol (2 v/v)
43.3 μl of water (100 v/v)
C11
  • Polyethylene glycol 4,000 25 w/v -
  • BIS-TRIS propane 0.1 M pH 6.5
  • di-Ammonium tartrate 0.2 M -
pH 6.5
100.0 μl of BIS-TRIS propane pH 6.5 (1 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of di-Ammonium tartrate (2 M)
300.0 μl of water (100 v/v)
C12
  • Polyethylene glycol 4,000 30 w/v -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
300.0 μl of water (100 v/v)
D1
  • Polyethylene glycol 4,000 30 w/v -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Ammonium acetate 0.2 M -
pH 5.6
40.0 μl of Ammonium acetate (5 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
260.0 μl of water (100 v/v)
D2
  • Polyethylene glycol 4,000 30 w/v -
  • tris chloride BCC 0.1 M pH 7.5
  • Cesium chloride 0.1 M -
  • Lithium sulfate 0.25 M -
pH 7.5
100.0 μl of Cesium chloride (1 M)
83.3 μl of Lithium sulfate (3 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
33.3 μl of tris chloride BCC pH 7.5 (3 M)
183.3 μl of water (100 v/v)
D3
  • Polyethylene glycol 4,000 35 w/v -
  • MES 0.1 M pH 6
pH 6
100.0 μl of MES pH 6 (1 M)
700.0 μl of Polyethylene glycol 4,000 (50 w/v)
200.0 μl of water (100 v/v)
D4
  • Polyethylene glycol 4,000 42 w/v -
  • tris chloride BCC 0.05 M pH 8
  • Sodium perchlorate 0.1 M -
pH 8
840.0 μl of Polyethylene glycol 4,000 (50 w/v)
50.0 μl of Sodium perchlorate (2 M)
50.0 μl of tris chloride BCC pH 8 (1 M)
60.0 μl of water (100 v/v)
D5
  • Polyethylene glycol 6,000 8 w/v -
  • tri-Sodium citrate 0.1 M pH 4
  • Ammonium di-hydrogen phosphate 0.05 M -
  • 2-Propanol 10 v/v -
pH 4
100.0 μl of 2-Propanol (100 v/v)
50.0 μl of Ammonium di-hydrogen phosphate (1 M)
160.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 4 (1 M)
590.0 μl of water (100 v/v)
D6
  • Polyethylene glycol 6,000 10 w/v -
  • 2-methyl-2,4-pentanediol BCC 4 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
40.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
660.0 μl of water (100 v/v)
D7
  • Polyethylene glycol 6,000 10 w/v -
  • MES 0.1 M pH 6.5
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
700.0 μl of water (100 v/v)
D8
  • Polyethylene glycol 6,000 12 w/v -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
240.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
660.0 μl of water (100 v/v)
D9
  • Polyethylene glycol 6,000 12 w/v -
  • TRIS-Acetate 0.1 M pH 8
pH 8
240.0 μl of Polyethylene glycol 6,000 (50 w/v)
50.0 μl of TRIS-Acetate pH 8 (2 M)
710.0 μl of water (100 v/v)
D10
  • Polyethylene glycol 6,000 12 w/v -
  • Glycerol 2 v/v -
  • di-Sodium hydrogen phosphate 0.025 M -
NA
20.0 μl of Glycerol (100 v/v)
240.0 μl of Polyethylene glycol 6,000 (50 w/v)
5.0 μl of di-Sodium hydrogen phosphate (5 M)
735.0 μl of water (100 v/v)
D11
  • Polyethylene glycol 6,000 15 w/v -
  • Sodium di-hydrogen phosphate 0.05 M pH 7
  • Potassium chloride 0.025 M -
  • Magnesium sulfate 0.005 M -
pH 7
5.0 μl of Magnesium sulfate (1 M)
300.0 μl of Polyethylene glycol 6,000 (50 w/v)
5.0 μl of Potassium chloride (5 M)
12.5 μl of Sodium di-hydrogen phosphate pH 7 (4 M)
677.5 μl of water (100 v/v)
D12
  • Polyethylene glycol 6,000 20 w/v -
  • BICINE 0.1 M pH 9
pH 9
100.0 μl of BICINE pH 9 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
500.0 μl of water (100 v/v)
E1
  • Polyethylene glycol 8,000 8 w/v -
  • Potassium di-hydrogen phosphate 0.1 M -
  • ß-Mercaptoethanol 0.2 v/v -
NA
160.0 μl of Polyethylene glycol 8,000 (50 w/v)
50.0 μl of Potassium di-hydrogen phosphate (2 M)
100.0 μl of ß-Mercaptoethanol (2 v/v)
690.0 μl of water (100 v/v)
E2
  • Polyethylene glycol 8,000 10 w/v -
  • HEPES 0.1 M pH 7.5
  • Magnesium acetate 0.2 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Magnesium acetate (2 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
600.0 μl of water (100 v/v)
E3
  • Polyethylene glycol 8,000 10 w/v -
  • MES 0.1 M pH 6.5
  • Potassium chloride 0.2 M -
  • Magnesium acetate 0.1 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
50.0 μl of Magnesium acetate (2 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
40.0 μl of Potassium chloride (5 M)
610.0 μl of water (100 v/v)
E4
  • Polyethylene glycol 8,000 10 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Sodium chloride 0.05 M -
pH 8.5
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
50.0 μl of Sodium chloride (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
650.0 μl of water (100 v/v)
E5
  • Polyethylene glycol 8,000 12 w/v -
  • Sodium acetate 0.05 M pH 5
  • Ammonium acetate 0.3 M -
pH 5
60.0 μl of Ammonium acetate (5 M)
240.0 μl of Polyethylene glycol 8,000 (50 w/v)
16.7 μl of Sodium acetate pH 5 (3 M)
683.3 μl of water (100 v/v)
E6
  • Polyethylene glycol 8,000 12 w/v -
  • tris chloride BCC 0.1 M pH 6.3
  • Lithium sulfate 0.2 M -
pH 6.3
66.7 μl of Lithium sulfate (3 M)
240.0 μl of Polyethylene glycol 8,000 (50 w/v)
33.3 μl of tris chloride BCC pH 6.3 (3 M)
660.0 μl of water (100 v/v)
E7
  • Polyethylene glycol 8,000 12 w/v -
  • HEPES 0.1 M pH 7.5
  • Sodium acetate 0.2 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
240.0 μl of Polyethylene glycol 8,000 (50 w/v)
80.0 μl of Sodium acetate (2.5 M)
580.0 μl of water (100 v/v)
E8
  • Polyethylene glycol 8,000 13 w/v -
  • Glycerol 15 v/v -
  • MOPS 0.1 M pH 7
  • Ammonium sulfate 0.5 M -
pH 7
166.7 μl of Ammonium sulfate (3 M)
150.0 μl of Glycerol (100 v/v)
100.0 μl of MOPS pH 7 (1 M)
260.0 μl of Polyethylene glycol 8,000 (50 w/v)
323.3 μl of water (100 v/v)
E9
  • Polyethylene glycol 8,000 15 w/v -
  • Potassium di-hydrogen phosphate 0.1 M pH 4.6
pH 4.6
300.0 μl of Polyethylene glycol 8,000 (50 w/v)
50.0 μl of Potassium di-hydrogen phosphate pH 4.6 (2 M)
650.0 μl of water (100 v/v)
E10
  • Polyethylene glycol 8,000 15 w/v -
  • Sodium di-hydrogen phosphate 0.1 M pH 4.6
pH 4.6
300.0 μl of Polyethylene glycol 8,000 (50 w/v)
25.0 μl of Sodium di-hydrogen phosphate pH 4.6 (4 M)
675.0 μl of water (100 v/v)
E11
  • Polyethylene glycol 8,000 15 w/v -
  • di-Sodium succinate 0.1 M pH 5.5
  • Lithium sulfate 0.25 M -
pH 5.5
83.3 μl of Lithium sulfate (3 M)
300.0 μl of Polyethylene glycol 8,000 (50 w/v)
33.3 μl of di-Sodium succinate pH 5.5 (3 M)
583.3 μl of water (100 v/v)
E12
  • Polyethylene glycol 8,000 18 w/v -
  • HEPES 0.1 M pH 7
  • Potassium di-hydrogen phosphate 0.1 M -
pH 7
100.0 μl of HEPES pH 7 (1 M)
360.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Potassium di-hydrogen phosphate pH 7 (1 M)
440.0 μl of water (100 v/v)
F1
  • Polyethylene glycol 8,000 18 w/v -
  • Lithium sulfate 0.2 M -
NA
66.7 μl of Lithium sulfate (3 M)
360.0 μl of Polyethylene glycol 8,000 (50 w/v)
573.3 μl of water (100 v/v)
F2
  • Polyethylene glycol 8,000 18 w/v -
  • 1,4-Dioxane 5 v/v -
  • tris chloride BCC 0.1 M pH 8
  • Sodium fluoride 0.2 M -
pH 8
100.0 μl of 1,4-Dioxane (50 v/v)
360.0 μl of Polyethylene glycol 8,000 (50 w/v)
250.0 μl of Sodium fluoride (0.8 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
190.0 μl of water (100 v/v)
F3
  • Polyethylene glycol 8,000 25 w/v -
  • Jeffamine® M-600 5 v/v -
  • MES 0.09 M pH 6
  • Magnesium acetate 0.09 M -
pH 6
100.0 μl of Jeffamine® M-600 (50 v/v)
30.0 μl of MES pH 6 (3 M)
45.0 μl of Magnesium acetate (2 M)
500.0 μl of Polyethylene glycol 8,000 (50 w/v)
325.0 μl of water (100 v/v)
F4
  • Polyethylene glycol 8,000 25 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Magnesium sulfate 0.2 M -
pH 8.5
100.0 μl of Magnesium sulfate (2 M)
500.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
F5
  • Polyethylene glycol 10,000 5 w/v -
  • HEPES 0.1 M pH 7.5
  • Magnesium chloride 0.2 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
80.0 μl of Magnesium chloride pH 7 (2.5 M)
100.0 μl of Polyethylene glycol 10,000 (50 w/v)
720.0 μl of water (100 v/v)
F6
  • Polyethylene glycol 10,000 10 w/v -
  • Sodium acetate 0.05 M pH 5
pH 5
200.0 μl of Polyethylene glycol 10,000 (50 w/v)
16.7 μl of Sodium acetate pH 5 (3 M)
783.3 μl of water (100 v/v)
F7
  • Polyethylene glycol 10,000 10 w/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 10,000 (50 w/v)
700.0 μl of water (100 v/v)
F8
  • Polyethylene glycol 10,000 15 w/v -
  • BIS-TRIS 0.1 M pH 5.5
  • Ammonium acetate 0.1 M -
  • n-Dodecyl-N,N-dimethylamine-N-oxide 5 mM -
pH 5.5
20.0 μl of Ammonium acetate (5 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
300.0 μl of Polyethylene glycol 10,000 (50 w/v)
50.0 μl of n-Dodecyl-N,N-dimethylamine-N-oxide (100 mM)
530.0 μl of water (100 v/v)
F9
  • Polyethylene glycol 10,000 15 w/v -
  • Glycine 0.06 M pH 9
  • tri-Sodium citrate 0.02 M -
pH 9
30.0 μl of Glycine pH 9 (2 M)
300.0 μl of Polyethylene glycol 10,000 (50 w/v)
10.0 μl of tri-Sodium citrate (2 M)
660.0 μl of water (100 v/v)
F10
  • Polyethylene glycol 10,000 20 w/v -
  • Polyethylene glycol monomethyl ether 550 5 v/v -
  • tri-Sodium citrate 0.1 M pH 5.6
pH 5.6
400.0 μl of Polyethylene glycol 10,000 (50 w/v)
50.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
450.0 μl of water (100 v/v)
F11
  • Polyethylene glycol 20,000 12 w/v -
  • MES 0.1 M pH 6.5
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
240.0 μl of Polyethylene glycol 20,000 (50 w/v)
660.0 μl of water (100 v/v)
F12
  • Polyethylene glycol 20,000 17 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Magnesium chloride 0.1 M -
pH 8.5
40.0 μl of Magnesium chloride pH 7 (2.5 M)
340.0 μl of Polyethylene glycol 20,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
520.0 μl of water (100 v/v)
G1
  • Ammonium sulfate 0.5 M -
  • HEPES 0.1 M pH 7.5
  • Lithium sulfate 0.2 M -
pH 7.5
166.7 μl of Ammonium sulfate (3 M)
100.0 μl of HEPES pH 7.5 (1 M)
66.7 μl of Lithium sulfate (3 M)
666.7 μl of water (100 v/v)
G2
  • Ammonium sulfate 1 M -
  • MES 0.1 M pH 6.5
  • Potassium chloride 0.1 M -
pH 6.5
333.3 μl of Ammonium sulfate (3 M)
100.0 μl of MES pH 6.5 (1 M)
20.0 μl of Potassium chloride (5 M)
546.7 μl of water (100 v/v)
G3
  • Ammonium sulfate 1 M -
  • Potassium chloride 0.8 M -
  • HEPES 0.1 M pH 7
pH 7
333.3 μl of Ammonium sulfate (3 M)
100.0 μl of HEPES pH 7 (1 M)
160.0 μl of Potassium chloride (5 M)
406.7 μl of water (100 v/v)
G4
  • Ammonium sulfate 1.3 M -
  • CHES 0.1 M pH 9.5
  • Sodium chloride 0.2 M -
pH 9.5
433.3 μl of Ammonium sulfate (3 M)
100.0 μl of CHES pH 9.5 (1 M)
200.0 μl of Sodium chloride (1 M)
266.7 μl of water (100 v/v)
G5
  • Ammonium sulfate 1.3 M -
  • Sodium acetate 0.1 M pH 5.5
pH 5.5
433.3 μl of Ammonium sulfate (3 M)
33.3 μl of Sodium acetate pH 5.5 (3 M)
533.3 μl of water (100 v/v)
G6
  • Ammonium sulfate 1.3 M -
  • Glycerol 5 v/v -
  • di-Sodium maleate 0.1 M pH 6.5
  • Magnesium chloride 0.001 M -
  • Zinc chloride 0.005 M -
pH 6.5
433.3 μl of Ammonium sulfate (3 M)
50.0 μl of Glycerol (100 v/v)
0.4 μl of Magnesium chloride pH 7 (2.5 M)
5.0 μl of Zinc chloride (1 M)
100.0 μl of di-Sodium maleate pH 6.5 (1 M)
411.3 μl of water (100 v/v)
G7
  • Ammonium sulfate 1.5 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Lithium sulfate 0.2 M -
pH 8.5
500.0 μl of Ammonium sulfate (3 M)
66.7 μl of Lithium sulfate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
333.3 μl of water (100 v/v)
G8
  • Ammonium sulfate 1.7 M -
  • MES 0.1 M pH 6
pH 6
566.7 μl of Ammonium sulfate (3 M)
33.3 μl of MES pH 6 (3 M)
400.0 μl of water (100 v/v)
G9
  • Ammonium sulfate 1.7 M -
  • 2-methyl-2,4-pentanediol BCC 4 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
40.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
566.7 μl of Ammonium sulfate (3 M)
100.0 μl of HEPES pH 7.5 (1 M)
293.3 μl of water (100 v/v)
G10
  • Ammonium sulfate 1.7 M -
  • Polyethylene glycol 400 2 v/v -
  • tris chloride BCC 0.1 M pH 7.5
pH 7.5
566.7 μl of Ammonium sulfate (3 M)
20.0 μl of Polyethylene glycol 400 (100 v/v)
33.3 μl of tris chloride BCC pH 7.5 (3 M)
380.0 μl of water (100 v/v)
G11
  • Ammonium sulfate 2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
666.7 μl of Ammonium sulfate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
233.3 μl of water (100 v/v)
G12
  • Ammonium sulfate 2 M -
  • Polyethylene glycol 400 10 v/v -
  • HEPES 0.05 M pH 7.5
  • Zinc sulfate 0.05 M -
pH 7.5
666.7 μl of Ammonium sulfate (3 M)
50.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Polyethylene glycol 400 (100 v/v)
50.0 μl of Zinc sulfate (1 M)
133.3 μl of water (100 v/v)
H1
  • Ammonium sulfate 2 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Potassium Sodium tartrate 0.2 M -
pH 5.6
666.7 μl of Ammonium sulfate (3 M)
100.0 μl of Potassium Sodium tartrate (2 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
133.3 μl of water (100 v/v)
H2
  • Ammonium sulfate 2.2 M -
  • Polypropylene glycol 400 6 v/v -
  • BIS-TRIS propane 0.1 M pH 6.5
pH 6.5
733.3 μl of Ammonium sulfate (3 M)
100.0 μl of BIS-TRIS propane pH 6.5 (1 M)
60.0 μl of Polypropylene glycol 400 (100 v/v)
106.7 μl of water (100 v/v)
H3
  • Ammonium sulfate 2.4 M -
  • HEPES 0.1 M pH 7
pH 7
800.0 μl of Ammonium sulfate (3 M)
100.0 μl of HEPES pH 7 (1 M)
100.0 μl of water (100 v/v)
H4
  • Ammonium sulfate 2.4 M -
  • tris chloride BCC 0.1 M pH 8
  • Magnesium chloride 0.01 M -
  • Zinc sulfate 0.005 M -
pH 8
800.0 μl of Ammonium sulfate (3 M)
10.0 μl of Magnesium chloride pH 7 (1 M)
5.0 μl of Zinc sulfate (1 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
85.0 μl of water (100 v/v)
H5
  • Lithium sulfate 0.5 M -
  • Polyethylene glycol 400 10 v/v -
  • Imidazole 0.1 M pH 8
pH 8
100.0 μl of Imidazole pH 8 (1 M)
166.7 μl of Lithium sulfate (3 M)
100.0 μl of Polyethylene glycol 400 (100 v/v)
633.3 μl of water (100 v/v)
H6
  • Lithium sulfate 1.5 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
500.0 μl of Lithium sulfate (3 M)
400.0 μl of water (100 v/v)
H7
  • Lithium sulfate 2 M -
  • Polyethylene glycol 400 2 v/v -
  • TRIS-Acetate 0.1 M pH 8
  • ß-Mercaptoethanol 0.1 v/v -
pH 8
666.7 μl of Lithium sulfate (3 M)
20.0 μl of Polyethylene glycol 400 (100 v/v)
50.0 μl of TRIS-Acetate pH 8 (2 M)
50.0 μl of ß-Mercaptoethanol (2 v/v)
213.3 μl of water (100 v/v)
H8
  • 2-methyl-2,4-pentanediol BCC 20 v/v -
  • MES 0.1 M pH 6.5
pH 6.5
200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of MES pH 6.5 (1 M)
700.0 μl of water (100 v/v)
H9
  • 2-methyl-2,4-pentanediol BCC 35 v/v -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
550.0 μl of water (100 v/v)
H10
  • Sodium formate 1.4 M -
  • MES 0.1 M pH 6
pH 6
33.3 μl of MES pH 6 (3 M)
200.0 μl of Sodium formate (7 M)
766.7 μl of water (100 v/v)
H11
  • Sodium formate 3 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
428.6 μl of Sodium formate (7 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
471.4 μl of water (100 v/v)
H12
  • di-Sodium tartrate 1 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
500.0 μl of di-Sodium tartrate (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)