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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
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Phosphatase HTS
Barcodes
Phosphatase HTS
No barcodes found.
Screen Structure
Phosphatase HTS
Well Count | 96 |
---|---|
Number of distinct ingredients | 68 |
Internal diversity (score mean dist) | 0.946 |
Screen Composition
ingredient type | Phosphatase HTS |
---|---|
Buffer | 116 |
Cryoprotectant | 5 |
Detergent | 1 |
Organic (non-volatile) | 20 |
Organic (volatile) | 6 |
Polymer | 79 |
Salt | 114 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 5.6 | 66.7 μl of Ammonium sulfate (3 M) 150.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of di-Sodium tartrate (2 M) 25.0 μl of tri-Sodium citrate pH 5.6 (2 M) 658.3 μl of water (100 v/v) |
A2 |
| pH 8.5 | 66.7 μl of Lithium sulfate (3 M) 220.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 613.3 μl of water (100 v/v) |
A3 |
| pH 7.5 | 200.0 μl of Cesium chloride (1 M) 100.0 μl of HEPES pH 7.5 (1 M) 250.0 μl of Polyethylene glycol 400 (100 v/v) 450.0 μl of water (100 v/v) |
A4 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 250.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 10.0 μl of Zinc sulfate (1 M) 640.0 μl of water (100 v/v) |
A5 |
| pH 7 | 100.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 10.0 μl of Imidazole pH 7 (1 M) 540.0 μl of Polyethylene glycol 1,500 (50 w/v) 31.4 μl of Sodium nitrate (7 M) 318.6 μl of water (100 v/v) |
A6 |
| pH 6.5 | 100.0 μl of BIS-TRIS propane pH 6.5 (1 M) 600.0 μl of Polyethylene glycol 1,500 (50 w/v) 66.7 μl of Potassium fluoride (6 M) 233.3 μl of water (100 v/v) |
A7 |
| pH 7 | 10.0 μl of Nickel (II) chloride pH 7 (1 M) 160.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 3.3 μl of tris chloride BCC pH 7 (3 M) 826.7 μl of water (100 v/v) |
A8 |
| pH 4.6 | 133.3 μl of Ammonium sulfate (3 M) 320.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 446.7 μl of water (100 v/v) |
A9 |
| NA | 160.0 μl of Polyethylene glycol 3,350 (50 w/v) 50.0 μl of Sodium acetate (2 M) 790.0 μl of water (100 v/v) |
A10 |
| pH 7.5 | 40.0 μl of Ammonium acetate (5 M) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 3,350 (50 w/v) 660.0 μl of water (100 v/v) |
A11 |
| pH 7.5 | 10.0 μl of Ammonium acetate (5 M) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 3,350 (50 w/v) 15.0 μl of Sodium chloride (1 M) 675.0 μl of water (100 v/v) |
A12 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 200.0 μl of Polyethylene glycol 3,350 (50 w/v) 10.0 μl of Zinc acetate (2 M) 690.0 μl of water (100 v/v) |
B1 |
| NA | 300.0 μl of Polyethylene glycol 3,350 (50 w/v) 250.0 μl of Sodium fluoride (0.8 M) 450.0 μl of water (100 v/v) |
B2 |
| pH 6.5 | 100.0 μl of BIS-TRIS propane pH 6.5 (1 M) 25.0 μl of Glycine (2 M) 300.0 μl of Polyethylene glycol 3,350 (50 w/v) 28.6 μl of Sodium formate (7 M) 546.4 μl of water (100 v/v) |
B3 |
| pH 7 | 100.0 μl of BIS-TRIS propane pH 7 (1 M) 50.0 μl of Glycerol (100 v/v) 200.0 μl of Magnesium formate (1 M) 300.0 μl of Polyethylene glycol 3,350 (50 w/v) 350.0 μl of water (100 v/v) |
B4 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 33.3 μl of Potassium fluoride (6 M) 566.7 μl of water (100 v/v) |
B5 |
| pH 6.5 | 20.0 μl of Ammonium chloride (5 M) 100.0 μl of MES pH 6.5 (1 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 480.0 μl of water (100 v/v) |
B6 |
| pH 8 | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 400.0 μl of Potassium sulfate (0.5 M) 10.0 μl of tris chloride BCC pH 8 (1 M) 190.0 μl of water (100 v/v) |
B7 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 50.0 μl of tri-Sodium citrate (2 M) 550.0 μl of water (100 v/v) |
B8 |
| pH 7.5 | 40.0 μl of Calcium chloride (5 M) 100.0 μl of HEPES pH 7.5 (1 M) 440.0 μl of Polyethylene glycol 3,350 (50 w/v) 420.0 μl of water (100 v/v) |
B9 |
| pH 8.5 | 80.0 μl of Magnesium chloride pH 7 (2.5 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 320.0 μl of water (100 v/v) |
B10 |
| pH 4.8 | 100.0 μl of 2-Propanol (100 v/v) 20.0 μl of Lithium chloride (5 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of tri-Sodium citrate pH 4.8 (1 M) 280.0 μl of water (100 v/v) |
B11 |
| NA | 20.0 μl of Ammonium nitrate (10 M) 600.0 μl of Polyethylene glycol 3,350 (50 w/v) 380.0 μl of water (100 v/v) |
B12 |
| NA | 66.7 μl of Ammonium di-hydrogen phosphate (3 M) 600.0 μl of Polyethylene glycol 3,350 (50 w/v) 333.3 μl of water (100 v/v) |
C1 |
| pH 7 | 50.0 μl of HEPES pH 7 (1 M) 7.3 μl of Lithium sulfate (3 M) 160.0 μl of Polyethylene glycol 4,000 (50 w/v) 50.0 μl of ß-Mercaptoethanol (2 v/v) 732.7 μl of water (100 v/v) |
C2 |
| pH 7.5 | 10.0 μl of Ammonium acetate (5 M) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 690.0 μl of water (100 v/v) |
C3 |
| pH 5.5 | 100.0 μl of Glycerol (100 v/v) 1.2 μl of Magnesium chloride pH 7 (2.5 M) 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 25.0 μl of TRIS-Phosphate (2 M) 16.7 μl of di-Sodium succinate pH 5.5 (3 M) 657.1 μl of water (100 v/v) |
C4 |
| pH 5.6 | 100.0 μl of 2-Propanol (100 v/v) 240.0 μl of Polyethylene glycol 4,000 (50 w/v) 25.0 μl of tri-Sodium citrate pH 5.6 (4 M) 635.0 μl of water (100 v/v) |
C5 |
| pH 6 | 100.0 μl of Imidazole-Malate pH 6 (2 M) 240.0 μl of Polyethylene glycol 4,000 (50 w/v) 1.0 μl of Zinc acetate (2 M) 659.0 μl of water (100 v/v) |
C6 |
| pH 7 | 100.0 μl of HEPES pH 7 (1 M) 80.0 μl of Magnesium chloride pH 7 (2.5 M) 300.0 μl of Polyethylene glycol 4,000 (50 w/v) 520.0 μl of water (100 v/v) |
C7 |
| pH 6.5 | 16.7 μl of Ammonium sulfate (3 M) 50.0 μl of MES pH 6.5 (1 M) 200.0 μl of Magnesium sulfate (1 M) 300.0 μl of Polyethylene glycol 4,000 (50 w/v) 150.0 μl of Polyethylene glycol 400 (100 v/v) 283.3 μl of water (100 v/v) |
C8 |
| pH 6.5 | 85.0 μl of MES pH 6.5 (1 M) 300.0 μl of Polyethylene glycol 4,000 (50 w/v) 85.0 μl of Sodium acetate (2 M) 530.0 μl of water (100 v/v) |
C9 |
| pH 9 | 50.0 μl of 2-Propanol (100 v/v) 100.0 μl of BICINE pH 9 (1 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 450.0 μl of water (100 v/v) |
C10 |
| pH 8.5 | 100.0 μl of 2-Propanol (100 v/v) 66.7 μl of Lithium sulfate (3 M) 440.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 250.0 μl of ß-Mercaptoethanol (2 v/v) 43.3 μl of water (100 v/v) |
C11 |
| pH 6.5 | 100.0 μl of BIS-TRIS propane pH 6.5 (1 M) 500.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of di-Ammonium tartrate (2 M) 300.0 μl of water (100 v/v) |
C12 |
| pH 4.6 | 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 300.0 μl of water (100 v/v) |
D1 |
| pH 5.6 | 40.0 μl of Ammonium acetate (5 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 260.0 μl of water (100 v/v) |
D2 |
| pH 7.5 | 100.0 μl of Cesium chloride (1 M) 83.3 μl of Lithium sulfate (3 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 33.3 μl of tris chloride BCC pH 7.5 (3 M) 183.3 μl of water (100 v/v) |
D3 |
| pH 6 | 100.0 μl of MES pH 6 (1 M) 700.0 μl of Polyethylene glycol 4,000 (50 w/v) 200.0 μl of water (100 v/v) |
D4 |
| pH 8 | 840.0 μl of Polyethylene glycol 4,000 (50 w/v) 50.0 μl of Sodium perchlorate (2 M) 50.0 μl of tris chloride BCC pH 8 (1 M) 60.0 μl of water (100 v/v) |
D5 |
| pH 4 | 100.0 μl of 2-Propanol (100 v/v) 50.0 μl of Ammonium di-hydrogen phosphate (1 M) 160.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 4 (1 M) 590.0 μl of water (100 v/v) |
D6 |
| pH 7.5 | 40.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 6,000 (50 w/v) 660.0 μl of water (100 v/v) |
D7 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 200.0 μl of Polyethylene glycol 6,000 (50 w/v) 700.0 μl of water (100 v/v) |
D8 |
| pH 4.6 | 240.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 660.0 μl of water (100 v/v) |
D9 |
| pH 8 | 240.0 μl of Polyethylene glycol 6,000 (50 w/v) 50.0 μl of TRIS-Acetate pH 8 (2 M) 710.0 μl of water (100 v/v) |
D10 |
| NA | 20.0 μl of Glycerol (100 v/v) 240.0 μl of Polyethylene glycol 6,000 (50 w/v) 5.0 μl of di-Sodium hydrogen phosphate (5 M) 735.0 μl of water (100 v/v) |
D11 |
| pH 7 | 5.0 μl of Magnesium sulfate (1 M) 300.0 μl of Polyethylene glycol 6,000 (50 w/v) 5.0 μl of Potassium chloride (5 M) 12.5 μl of Sodium di-hydrogen phosphate pH 7 (4 M) 677.5 μl of water (100 v/v) |
D12 |
| pH 9 | 100.0 μl of BICINE pH 9 (1 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 500.0 μl of water (100 v/v) |
E1 |
| NA | 160.0 μl of Polyethylene glycol 8,000 (50 w/v) 50.0 μl of Potassium di-hydrogen phosphate (2 M) 100.0 μl of ß-Mercaptoethanol (2 v/v) 690.0 μl of water (100 v/v) |
E2 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 100.0 μl of Magnesium acetate (2 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 600.0 μl of water (100 v/v) |
E3 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 50.0 μl of Magnesium acetate (2 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 40.0 μl of Potassium chloride (5 M) 610.0 μl of water (100 v/v) |
E4 |
| pH 8.5 | 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 50.0 μl of Sodium chloride (1 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 650.0 μl of water (100 v/v) |
E5 |
| pH 5 | 60.0 μl of Ammonium acetate (5 M) 240.0 μl of Polyethylene glycol 8,000 (50 w/v) 16.7 μl of Sodium acetate pH 5 (3 M) 683.3 μl of water (100 v/v) |
E6 |
| pH 6.3 | 66.7 μl of Lithium sulfate (3 M) 240.0 μl of Polyethylene glycol 8,000 (50 w/v) 33.3 μl of tris chloride BCC pH 6.3 (3 M) 660.0 μl of water (100 v/v) |
E7 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 240.0 μl of Polyethylene glycol 8,000 (50 w/v) 80.0 μl of Sodium acetate (2.5 M) 580.0 μl of water (100 v/v) |
E8 |
| pH 7 | 166.7 μl of Ammonium sulfate (3 M) 150.0 μl of Glycerol (100 v/v) 100.0 μl of MOPS pH 7 (1 M) 260.0 μl of Polyethylene glycol 8,000 (50 w/v) 323.3 μl of water (100 v/v) |
E9 |
| pH 4.6 | 300.0 μl of Polyethylene glycol 8,000 (50 w/v) 50.0 μl of Potassium di-hydrogen phosphate pH 4.6 (2 M) 650.0 μl of water (100 v/v) |
E10 |
| pH 4.6 | 300.0 μl of Polyethylene glycol 8,000 (50 w/v) 25.0 μl of Sodium di-hydrogen phosphate pH 4.6 (4 M) 675.0 μl of water (100 v/v) |
E11 |
| pH 5.5 | 83.3 μl of Lithium sulfate (3 M) 300.0 μl of Polyethylene glycol 8,000 (50 w/v) 33.3 μl of di-Sodium succinate pH 5.5 (3 M) 583.3 μl of water (100 v/v) |
E12 |
| pH 7 | 100.0 μl of HEPES pH 7 (1 M) 360.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Potassium di-hydrogen phosphate pH 7 (1 M) 440.0 μl of water (100 v/v) |
F1 |
| NA | 66.7 μl of Lithium sulfate (3 M) 360.0 μl of Polyethylene glycol 8,000 (50 w/v) 573.3 μl of water (100 v/v) |
F2 |
| pH 8 | 100.0 μl of 1,4-Dioxane (50 v/v) 360.0 μl of Polyethylene glycol 8,000 (50 w/v) 250.0 μl of Sodium fluoride (0.8 M) 100.0 μl of tris chloride BCC pH 8 (1 M) 190.0 μl of water (100 v/v) |
F3 |
| pH 6 | 100.0 μl of Jeffamine® M-600 (50 v/v) 30.0 μl of MES pH 6 (3 M) 45.0 μl of Magnesium acetate (2 M) 500.0 μl of Polyethylene glycol 8,000 (50 w/v) 325.0 μl of water (100 v/v) |
F4 |
| pH 8.5 | 100.0 μl of Magnesium sulfate (2 M) 500.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 300.0 μl of water (100 v/v) |
F5 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 80.0 μl of Magnesium chloride pH 7 (2.5 M) 100.0 μl of Polyethylene glycol 10,000 (50 w/v) 720.0 μl of water (100 v/v) |
F6 |
| pH 5 | 200.0 μl of Polyethylene glycol 10,000 (50 w/v) 16.7 μl of Sodium acetate pH 5 (3 M) 783.3 μl of water (100 v/v) |
F7 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 10,000 (50 w/v) 700.0 μl of water (100 v/v) |
F8 |
| pH 5.5 | 20.0 μl of Ammonium acetate (5 M) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 300.0 μl of Polyethylene glycol 10,000 (50 w/v) 50.0 μl of n-Dodecyl-N,N-dimethylamine-N-oxide (100 mM) 530.0 μl of water (100 v/v) |
F9 |
| pH 9 | 30.0 μl of Glycine pH 9 (2 M) 300.0 μl of Polyethylene glycol 10,000 (50 w/v) 10.0 μl of tri-Sodium citrate (2 M) 660.0 μl of water (100 v/v) |
F10 |
| pH 5.6 | 400.0 μl of Polyethylene glycol 10,000 (50 w/v) 50.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 450.0 μl of water (100 v/v) |
F11 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 240.0 μl of Polyethylene glycol 20,000 (50 w/v) 660.0 μl of water (100 v/v) |
F12 |
| pH 8.5 | 40.0 μl of Magnesium chloride pH 7 (2.5 M) 340.0 μl of Polyethylene glycol 20,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 520.0 μl of water (100 v/v) |
G1 |
| pH 7.5 | 166.7 μl of Ammonium sulfate (3 M) 100.0 μl of HEPES pH 7.5 (1 M) 66.7 μl of Lithium sulfate (3 M) 666.7 μl of water (100 v/v) |
G2 |
| pH 6.5 | 333.3 μl of Ammonium sulfate (3 M) 100.0 μl of MES pH 6.5 (1 M) 20.0 μl of Potassium chloride (5 M) 546.7 μl of water (100 v/v) |
G3 |
| pH 7 | 333.3 μl of Ammonium sulfate (3 M) 100.0 μl of HEPES pH 7 (1 M) 160.0 μl of Potassium chloride (5 M) 406.7 μl of water (100 v/v) |
G4 |
| pH 9.5 | 433.3 μl of Ammonium sulfate (3 M) 100.0 μl of CHES pH 9.5 (1 M) 200.0 μl of Sodium chloride (1 M) 266.7 μl of water (100 v/v) |
G5 |
| pH 5.5 | 433.3 μl of Ammonium sulfate (3 M) 33.3 μl of Sodium acetate pH 5.5 (3 M) 533.3 μl of water (100 v/v) |
G6 |
| pH 6.5 | 433.3 μl of Ammonium sulfate (3 M) 50.0 μl of Glycerol (100 v/v) 0.4 μl of Magnesium chloride pH 7 (2.5 M) 5.0 μl of Zinc chloride (1 M) 100.0 μl of di-Sodium maleate pH 6.5 (1 M) 411.3 μl of water (100 v/v) |
G7 |
| pH 8.5 | 500.0 μl of Ammonium sulfate (3 M) 66.7 μl of Lithium sulfate (3 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 333.3 μl of water (100 v/v) |
G8 |
| pH 6 | 566.7 μl of Ammonium sulfate (3 M) 33.3 μl of MES pH 6 (3 M) 400.0 μl of water (100 v/v) |
G9 |
| pH 7.5 | 40.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 566.7 μl of Ammonium sulfate (3 M) 100.0 μl of HEPES pH 7.5 (1 M) 293.3 μl of water (100 v/v) |
G10 |
| pH 7.5 | 566.7 μl of Ammonium sulfate (3 M) 20.0 μl of Polyethylene glycol 400 (100 v/v) 33.3 μl of tris chloride BCC pH 7.5 (3 M) 380.0 μl of water (100 v/v) |
G11 |
| pH 8.5 | 666.7 μl of Ammonium sulfate (3 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 233.3 μl of water (100 v/v) |
G12 |
| pH 7.5 | 666.7 μl of Ammonium sulfate (3 M) 50.0 μl of HEPES pH 7.5 (1 M) 100.0 μl of Polyethylene glycol 400 (100 v/v) 50.0 μl of Zinc sulfate (1 M) 133.3 μl of water (100 v/v) |
H1 |
| pH 5.6 | 666.7 μl of Ammonium sulfate (3 M) 100.0 μl of Potassium Sodium tartrate (2 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 133.3 μl of water (100 v/v) |
H2 |
| pH 6.5 | 733.3 μl of Ammonium sulfate (3 M) 100.0 μl of BIS-TRIS propane pH 6.5 (1 M) 60.0 μl of Polypropylene glycol 400 (100 v/v) 106.7 μl of water (100 v/v) |
H3 |
| pH 7 | 800.0 μl of Ammonium sulfate (3 M) 100.0 μl of HEPES pH 7 (1 M) 100.0 μl of water (100 v/v) |
H4 |
| pH 8 | 800.0 μl of Ammonium sulfate (3 M) 10.0 μl of Magnesium chloride pH 7 (1 M) 5.0 μl of Zinc sulfate (1 M) 100.0 μl of tris chloride BCC pH 8 (1 M) 85.0 μl of water (100 v/v) |
H5 |
| pH 8 | 100.0 μl of Imidazole pH 8 (1 M) 166.7 μl of Lithium sulfate (3 M) 100.0 μl of Polyethylene glycol 400 (100 v/v) 633.3 μl of water (100 v/v) |
H6 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 500.0 μl of Lithium sulfate (3 M) 400.0 μl of water (100 v/v) |
H7 |
| pH 8 | 666.7 μl of Lithium sulfate (3 M) 20.0 μl of Polyethylene glycol 400 (100 v/v) 50.0 μl of TRIS-Acetate pH 8 (2 M) 50.0 μl of ß-Mercaptoethanol (2 v/v) 213.3 μl of water (100 v/v) |
H8 |
| pH 6.5 | 200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of MES pH 6.5 (1 M) 700.0 μl of water (100 v/v) |
H9 |
| pH 4.6 | 350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 550.0 μl of water (100 v/v) |
H10 |
| pH 6 | 33.3 μl of MES pH 6 (3 M) 200.0 μl of Sodium formate (7 M) 766.7 μl of water (100 v/v) |
H11 |
| pH 8 | 428.6 μl of Sodium formate (7 M) 100.0 μl of tris chloride BCC pH 8 (1 M) 471.4 μl of water (100 v/v) |
H12 |
| pH 8.5 | 500.0 μl of di-Sodium tartrate (2 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 400.0 μl of water (100 v/v) |