C6 - Screen Contents - Phosphatase HTS

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Screen Description

Phosphatase HTS

Phosphatase / Phosphatase HTS

Created 2009-11-16.

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Phosphatase HTS

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Phosphatase HTS

Screen Structure

Phosphatase HTS

Well Count	96
Number of distinct ingredients	68
Internal diversity (score mean dist)	0.946

Screen Composition

More information about screen composition chart.

ingredient type	Phosphatase HTS
Buffer	116
Cryoprotectant	5
Detergent	1
Organic (non-volatile)	20
Organic (volatile)	6
Polymer	79
Salt	114

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Phosphatase HTS Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Polyethylene glycol 400 15 v/v - tri-Sodium citrate 0.05 M pH 5.6 Ammonium sulfate 0.2 M - di-Sodium tartrate 0.2 M - 🔍	pH 5.6	150 μl of Polyethylene glycol 400 (100 v/v) 66 μl of Ammonium sulfate (3 M) 25 μl of tri-Sodium citrate pH 5.6 (2 M) 100 μl of di-Sodium tartrate (2 M) 658 μl of water (100 v/v)
A2	Polyethylene glycol 400 22 v/v - tris chloride BCC 0.1 M pH 8.5 Lithium sulfate 0.2 M - 🔍	pH 8.5	220 μl of Polyethylene glycol 400 (100 v/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 66 μl of Lithium sulfate (3 M) 613 μl of water (100 v/v)
A3	Polyethylene glycol 400 25 v/v - HEPES 0.1 M pH 7.5 Cesium chloride 0.2 M - 🔍	pH 7.5	200 μl of Cesium chloride (1 M) 250 μl of Polyethylene glycol 400 (100 v/v) 100 μl of HEPES pH 7.5 (1 M) 450 μl of water (100 v/v)
A4	Polyethylene glycol monomethyl ether 550 25 v/v - MES 0.1 M pH 6.5 Zinc sulfate 0.01 M - 🔍	pH 6.5	10 μl of Zinc sulfate (1 M) 100 μl of MES pH 6.5 (1 M) 250 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 640 μl of water (100 v/v)
A5	Polyethylene glycol 1,500 27 w/v - 2-methyl-2,4-pentanediol BCC 10 v/v - Imidazole 0.01 M pH 7 Sodium nitrate 0.22 M - 🔍	pH 7	31 μl of Sodium nitrate (7 M) 540 μl of Polyethylene glycol 1,500 (50 w/v) 100 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 10 μl of Imidazole pH 7 (1 M) 318 μl of water (100 v/v)
A6	Polyethylene glycol 1,500 30 w/v - BIS-TRIS propane 0.1 M pH 6.5 Potassium fluoride 0.4 M - 🔍	pH 6.5	66 μl of Potassium fluoride (6 M) 600 μl of Polyethylene glycol 1,500 (50 w/v) 100 μl of BIS-TRIS propane pH 6.5 (1 M) 233 μl of water (100 v/v)
A7	Polyethylene glycol monomethyl ether 2,000 8 w/v - tris chloride BCC 0.01 M pH 7 Nickel (II) chloride 0.01 M - 🔍	pH 7	160 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 10 μl of Nickel (II) chloride pH 7 (1 M) 3 μl of tris chloride BCC pH 7 (3 M) 826 μl of water (100 v/v)
A8	Polyethylene glycol monomethyl ether 2,000 16 w/v - Sodium acetate 0.1 M pH 4.6 Ammonium sulfate 0.4 M - 🔍	pH 4.6	320 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 133 μl of Ammonium sulfate (3 M) 100 μl of Sodium acetate pH 4.6 (1 M) 446 μl of water (100 v/v)
A9	Polyethylene glycol 3,350 8 w/v - Sodium acetate 0.1 M - 🔍	NA	50 μl of Sodium acetate (2 M) 160 μl of Polyethylene glycol 3,350 (50 w/v) 790 μl of water (100 v/v)
A10	Polyethylene glycol 3,350 10 w/v - HEPES 0.1 M pH 7.5 Ammonium acetate 0.2 M - 🔍	pH 7.5	40 μl of Ammonium acetate (5 M) 100 μl of HEPES pH 7.5 (1 M) 200 μl of Polyethylene glycol 3,350 (50 w/v) 660 μl of water (100 v/v)
A11	Polyethylene glycol 3,350 10 w/v - HEPES 0.1 M pH 7.5 Ammonium acetate 0.05 M - Sodium chloride 0.015 M - 🔍	pH 7.5	10 μl of Ammonium acetate (5 M) 100 μl of HEPES pH 7.5 (1 M) 15 μl of Sodium chloride (1 M) 200 μl of Polyethylene glycol 3,350 (50 w/v) 675 μl of water (100 v/v)
A12	Polyethylene glycol 3,350 10 w/v - MES 0.1 M pH 6.5 Zinc acetate 0.02 M - 🔍	pH 6.5	10 μl of Zinc acetate (2 M) 100 μl of MES pH 6.5 (1 M) 200 μl of Polyethylene glycol 3,350 (50 w/v) 690 μl of water (100 v/v)
B1	Polyethylene glycol 3,350 15 w/v - Sodium fluoride 0.2 M - 🔍	NA	250 μl of Sodium fluoride (0.8 M) 300 μl of Polyethylene glycol 3,350 (50 w/v) 450 μl of water (100 v/v)
B2	Polyethylene glycol 3,350 15 w/v - BIS-TRIS propane 0.1 M pH 6.5 Glycine 0.05 M - Sodium formate 0.2 M - 🔍	pH 6.5	28 μl of Sodium formate (7 M) 25 μl of Glycine (2 M) 100 μl of BIS-TRIS propane pH 6.5 (1 M) 300 μl of Polyethylene glycol 3,350 (50 w/v) 546 μl of water (100 v/v)
B3	Polyethylene glycol 3,350 15 w/v - Glycerol 5 v/v - BIS-TRIS propane 0.1 M pH 7 Magnesium formate 0.2 M - 🔍	pH 7	200 μl of Magnesium formate (1 M) 50 μl of Glycerol (100 v/v) 100 μl of BIS-TRIS propane pH 7 (1 M) 300 μl of Polyethylene glycol 3,350 (50 w/v) 350 μl of water (100 v/v)
B4	Polyethylene glycol 3,350 20 w/v - Potassium fluoride 0.2 M - 🔍	NA	33 μl of Potassium fluoride (6 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 566 μl of water (100 v/v)
B5	Polyethylene glycol 3,350 20 w/v - MES 0.1 M pH 6.5 Ammonium chloride 0.1 M - 🔍	pH 6.5	20 μl of Ammonium chloride (5 M) 100 μl of MES pH 6.5 (1 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 480 μl of water (100 v/v)
B6	Polyethylene glycol 3,350 20 w/v - tris chloride BCC 0.01 M pH 8 Potassium sulfate 0.2 M - 🔍	pH 8	400 μl of Potassium sulfate (0.5 M) 10 μl of tris chloride BCC pH 8 (1 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 190 μl of water (100 v/v)
B7	Polyethylene glycol 3,350 20 w/v - tri-Sodium citrate 0.1 M - 🔍	NA	50 μl of tri-Sodium citrate (2 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 550 μl of water (100 v/v)
B8	Polyethylene glycol 3,350 22 w/v - HEPES 0.1 M pH 7.5 Calcium chloride 0.2 M - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 40 μl of Calcium chloride (5 M) 440 μl of Polyethylene glycol 3,350 (50 w/v) 420 μl of water (100 v/v)
B9	Polyethylene glycol 3,350 25 w/v - tris chloride BCC 0.1 M pH 8.5 Magnesium chloride 0.2 M - 🔍	pH 8.5	80 μl of Magnesium chloride pH 7 (2.5 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 500 μl of Polyethylene glycol 3,350 (50 w/v) 320 μl of water (100 v/v)
B10	Polyethylene glycol 3,350 25 w/v - 2-Propanol 10 v/v - tri-Sodium citrate 0.1 M pH 4.8 Lithium chloride 0.1 M - 🔍	pH 4.8	100 μl of tri-Sodium citrate pH 4.8 (1 M) 100 μl of 2-Propanol (100 v/v) 20 μl of Lithium chloride (5 M) 500 μl of Polyethylene glycol 3,350 (50 w/v) 280 μl of water (100 v/v)
B11	Polyethylene glycol 3,350 30 w/v - Ammonium nitrate 0.2 M - 🔍	NA	20 μl of Ammonium nitrate (10 M) 600 μl of Polyethylene glycol 3,350 (50 w/v) 380 μl of water (100 v/v)
B12	Polyethylene glycol 3,350 30 w/v - Ammonium di-hydrogen phosphate 0.2 M - 🔍	NA	66 μl of Ammonium di-hydrogen phosphate (3 M) 600 μl of Polyethylene glycol 3,350 (50 w/v) 333 μl of water (100 v/v)
C1	Polyethylene glycol 4,000 8 w/v - HEPES 0.05 M pH 7 Lithium sulfate 0.022 M - ß-Mercaptoethanol 0.1 v/v - 🔍	pH 7	50 μl of ß-Mercaptoethanol (2 v/v) 160 μl of Polyethylene glycol 4,000 (50 w/v) 50 μl of HEPES pH 7 (1 M) 7 μl of Lithium sulfate (3 M) 732 μl of water (100 v/v)
C2	Polyethylene glycol 4,000 10 w/v - HEPES 0.1 M pH 7.5 Ammonium acetate 0.05 M - 🔍	pH 7.5	10 μl of Ammonium acetate (5 M) 100 μl of HEPES pH 7.5 (1 M) 200 μl of Polyethylene glycol 4,000 (50 w/v) 690 μl of water (100 v/v)
C3	Polyethylene glycol 4,000 10 w/v - Glycerol 10 v/v - di-Sodium succinate 0.05 M pH 5.5 Magnesium chloride 0.003 M - TRIS-Phosphate 0.05 M - 🔍	pH 5.5	25 μl of TRIS-Phosphate (2 M) 100 μl of Glycerol (100 v/v) 1 μl of Magnesium chloride pH 7 (2.5 M) 16 μl of di-Sodium succinate pH 5.5 (3 M) 200 μl of Polyethylene glycol 4,000 (50 w/v) 657 μl of water (100 v/v)
C4	Polyethylene glycol 4,000 12 w/v - tri-Sodium citrate 0.1 M pH 5.6 2-Propanol 10 v/v - 🔍	pH 5.6	100 μl of 2-Propanol (100 v/v) 240 μl of Polyethylene glycol 4,000 (50 w/v) 25 μl of tri-Sodium citrate pH 5.6 (4 M) 635 μl of water (100 v/v)
C5	Polyethylene glycol 4,000 12 w/v - Imidazole-Malate 0.2 M pH 6 Zinc acetate 0.002 M - 🔍	pH 6	1 μl of Zinc acetate (2 M) 100 μl of Imidazole-Malate pH 6 (2 M) 240 μl of Polyethylene glycol 4,000 (50 w/v) 659 μl of water (100 v/v)
C6	Polyethylene glycol 4,000 15 w/v - HEPES 0.1 M pH 7 Magnesium chloride 0.2 M - 🔍	pH 7	80 μl of Magnesium chloride pH 7 (2.5 M) 300 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of HEPES pH 7 (1 M) 520 μl of water (100 v/v)
C7	Polyethylene glycol 4,000 15 w/v - Polyethylene glycol 400 15 v/v - MES 0.05 M pH 6.5 Magnesium sulfate 0.2 M - Ammonium sulfate 0.05 M - 🔍	pH 6.5	200 μl of Magnesium sulfate (1 M) 150 μl of Polyethylene glycol 400 (100 v/v) 16 μl of Ammonium sulfate (3 M) 50 μl of MES pH 6.5 (1 M) 300 μl of Polyethylene glycol 4,000 (50 w/v) 283 μl of water (100 v/v)
C8	Polyethylene glycol 4,000 15 w/v - MES 0.085 M pH 6.5 Sodium acetate 0.17 M - 🔍	pH 6.5	85 μl of Sodium acetate (2 M) 85 μl of MES pH 6.5 (1 M) 300 μl of Polyethylene glycol 4,000 (50 w/v) 530 μl of water (100 v/v)
C9	Polyethylene glycol 4,000 20 w/v - BICINE 0.1 M pH 9 2-Propanol 5 v/v - 🔍	pH 9	100 μl of BICINE pH 9 (1 M) 50 μl of 2-Propanol (100 v/v) 400 μl of Polyethylene glycol 4,000 (50 w/v) 450 μl of water (100 v/v)
C10	Polyethylene glycol 4,000 22 w/v - tris chloride BCC 0.1 M pH 8.5 Lithium sulfate 0.2 M - 2-Propanol 10 v/v - ß-Mercaptoethanol 0.5 v/v - 🔍	pH 8.5	100 μl of 2-Propanol (100 v/v) 250 μl of ß-Mercaptoethanol (2 v/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 440 μl of Polyethylene glycol 4,000 (50 w/v) 66 μl of Lithium sulfate (3 M) 43 μl of water (100 v/v)
C11	Polyethylene glycol 4,000 25 w/v - BIS-TRIS propane 0.1 M pH 6.5 di-Ammonium tartrate 0.2 M - 🔍	pH 6.5	100 μl of di-Ammonium tartrate (2 M) 500 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of BIS-TRIS propane pH 6.5 (1 M) 300 μl of water (100 v/v)
C12	Polyethylene glycol 4,000 30 w/v - Sodium acetate 0.1 M pH 4.6 🔍	pH 4.6	600 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of Sodium acetate pH 4.6 (1 M) 300 μl of water (100 v/v)
D1	Polyethylene glycol 4,000 30 w/v - tri-Sodium citrate 0.1 M pH 5.6 Ammonium acetate 0.2 M - 🔍	pH 5.6	40 μl of Ammonium acetate (5 M) 100 μl of tri-Sodium citrate pH 5.6 (1 M) 600 μl of Polyethylene glycol 4,000 (50 w/v) 260 μl of water (100 v/v)
D2	Polyethylene glycol 4,000 30 w/v - tris chloride BCC 0.1 M pH 7.5 Cesium chloride 0.1 M - Lithium sulfate 0.25 M - 🔍	pH 7.5	100 μl of Cesium chloride (1 M) 33 μl of tris chloride BCC pH 7.5 (3 M) 600 μl of Polyethylene glycol 4,000 (50 w/v) 83 μl of Lithium sulfate (3 M) 183 μl of water (100 v/v)
D3	Polyethylene glycol 4,000 35 w/v - MES 0.1 M pH 6 🔍	pH 6	100 μl of MES pH 6 (1 M) 700 μl of Polyethylene glycol 4,000 (50 w/v) 200 μl of water (100 v/v)
D4	Polyethylene glycol 4,000 42 w/v - tris chloride BCC 0.05 M pH 8 Sodium perchlorate 0.1 M - 🔍	pH 8	50 μl of Sodium perchlorate (2 M) 840 μl of Polyethylene glycol 4,000 (50 w/v) 50 μl of tris chloride BCC pH 8 (1 M) 60 μl of water (100 v/v)
D5	Polyethylene glycol 6,000 8 w/v - tri-Sodium citrate 0.1 M pH 4 Ammonium di-hydrogen phosphate 0.05 M - 2-Propanol 10 v/v - 🔍	pH 4	160 μl of Polyethylene glycol 6,000 (50 w/v) 100 μl of 2-Propanol (100 v/v) 50 μl of Ammonium di-hydrogen phosphate (1 M) 100 μl of tri-Sodium citrate pH 4 (1 M) 590 μl of water (100 v/v)
D6	Polyethylene glycol 6,000 10 w/v - 2-methyl-2,4-pentanediol BCC 4 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	200 μl of Polyethylene glycol 6,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 40 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 660 μl of water (100 v/v)
D7	Polyethylene glycol 6,000 10 w/v - MES 0.1 M pH 6.5 🔍	pH 6.5	200 μl of Polyethylene glycol 6,000 (50 w/v) 100 μl of MES pH 6.5 (1 M) 700 μl of water (100 v/v)
D8	Polyethylene glycol 6,000 12 w/v - Sodium acetate 0.1 M pH 4.6 🔍	pH 4.6	240 μl of Polyethylene glycol 6,000 (50 w/v) 100 μl of Sodium acetate pH 4.6 (1 M) 660 μl of water (100 v/v)
D9	Polyethylene glycol 6,000 12 w/v - TRIS-Acetate 0.1 M pH 8 🔍	pH 8	240 μl of Polyethylene glycol 6,000 (50 w/v) 50 μl of TRIS-Acetate pH 8 (2 M) 710 μl of water (100 v/v)
D10	Polyethylene glycol 6,000 12 w/v - Glycerol 2 v/v - di-Sodium hydrogen phosphate 0.025 M - 🔍	NA	20 μl of Glycerol (100 v/v) 240 μl of Polyethylene glycol 6,000 (50 w/v) 5 μl of di-Sodium hydrogen phosphate (5 M) 735 μl of water (100 v/v)
D11	Polyethylene glycol 6,000 15 w/v - Sodium di-hydrogen phosphate 0.05 M pH 7 Potassium chloride 0.025 M - Magnesium sulfate 0.005 M - 🔍	pH 7	5 μl of Magnesium sulfate (1 M) 12 μl of Sodium di-hydrogen phosphate pH 7 (4 M) 300 μl of Polyethylene glycol 6,000 (50 w/v) 5 μl of Potassium chloride (5 M) 677 μl of water (100 v/v)
D12	Polyethylene glycol 6,000 20 w/v - BICINE 0.1 M pH 9 🔍	pH 9	100 μl of BICINE pH 9 (1 M) 400 μl of Polyethylene glycol 6,000 (50 w/v) 500 μl of water (100 v/v)
E1	Polyethylene glycol 8,000 8 w/v - Potassium di-hydrogen phosphate 0.1 M - ß-Mercaptoethanol 0.2 v/v - 🔍	NA	160 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of ß-Mercaptoethanol (2 v/v) 50 μl of Potassium di-hydrogen phosphate (2 M) 690 μl of water (100 v/v)
E2	Polyethylene glycol 8,000 10 w/v - HEPES 0.1 M pH 7.5 Magnesium acetate 0.2 M - 🔍	pH 7.5	200 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of Magnesium acetate (2 M) 100 μl of HEPES pH 7.5 (1 M) 600 μl of water (100 v/v)
E3	Polyethylene glycol 8,000 10 w/v - MES 0.1 M pH 6.5 Potassium chloride 0.2 M - Magnesium acetate 0.1 M - 🔍	pH 6.5	200 μl of Polyethylene glycol 8,000 (50 w/v) 50 μl of Magnesium acetate (2 M) 40 μl of Potassium chloride (5 M) 100 μl of MES pH 6.5 (1 M) 610 μl of water (100 v/v)
E4	Polyethylene glycol 8,000 10 w/v - tris chloride BCC 0.1 M pH 8.5 Sodium chloride 0.05 M - 🔍	pH 8.5	200 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 50 μl of Sodium chloride (1 M) 650 μl of water (100 v/v)
E5	Polyethylene glycol 8,000 12 w/v - Sodium acetate 0.05 M pH 5 Ammonium acetate 0.3 M - 🔍	pH 5	60 μl of Ammonium acetate (5 M) 240 μl of Polyethylene glycol 8,000 (50 w/v) 16 μl of Sodium acetate pH 5 (3 M) 683 μl of water (100 v/v)
E6	Polyethylene glycol 8,000 12 w/v - tris chloride BCC 0.1 M pH 6.3 Lithium sulfate 0.2 M - 🔍	pH 6.3	33 μl of tris chloride BCC pH 6.3 (3 M) 240 μl of Polyethylene glycol 8,000 (50 w/v) 66 μl of Lithium sulfate (3 M) 660 μl of water (100 v/v)
E7	Polyethylene glycol 8,000 12 w/v - HEPES 0.1 M pH 7.5 Sodium acetate 0.2 M - 🔍	pH 7.5	240 μl of Polyethylene glycol 8,000 (50 w/v) 80 μl of Sodium acetate (2.5 M) 100 μl of HEPES pH 7.5 (1 M) 580 μl of water (100 v/v)
E8	Polyethylene glycol 8,000 13 w/v - Glycerol 15 v/v - MOPS 0.1 M pH 7 Ammonium sulfate 0.5 M - 🔍	pH 7	150 μl of Glycerol (100 v/v) 260 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of MOPS pH 7 (1 M) 166 μl of Ammonium sulfate (3 M) 323 μl of water (100 v/v)
E9	Polyethylene glycol 8,000 15 w/v - Potassium di-hydrogen phosphate 0.1 M pH 4.6 🔍	pH 4.6	50 μl of Potassium di-hydrogen phosphate pH 4.6 (2 M) 300 μl of Polyethylene glycol 8,000 (50 w/v) 650 μl of water (100 v/v)
E10	Polyethylene glycol 8,000 15 w/v - Sodium di-hydrogen phosphate 0.1 M pH 4.6 🔍	pH 4.6	300 μl of Polyethylene glycol 8,000 (50 w/v) 25 μl of Sodium di-hydrogen phosphate pH 4.6 (4 M) 675 μl of water (100 v/v)
E11	Polyethylene glycol 8,000 15 w/v - di-Sodium succinate 0.1 M pH 5.5 Lithium sulfate 0.25 M - 🔍	pH 5.5	300 μl of Polyethylene glycol 8,000 (50 w/v) 33 μl of di-Sodium succinate pH 5.5 (3 M) 83 μl of Lithium sulfate (3 M) 583 μl of water (100 v/v)
E12	Polyethylene glycol 8,000 18 w/v - HEPES 0.1 M pH 7 Potassium di-hydrogen phosphate 0.1 M - 🔍	pH 7	360 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of Potassium di-hydrogen phosphate pH 7 (1 M) 100 μl of HEPES pH 7 (1 M) 440 μl of water (100 v/v)
F1	Polyethylene glycol 8,000 18 w/v - Lithium sulfate 0.2 M - 🔍	NA	360 μl of Polyethylene glycol 8,000 (50 w/v) 66 μl of Lithium sulfate (3 M) 573 μl of water (100 v/v)
F2	Polyethylene glycol 8,000 18 w/v - 1,4-Dioxane 5 v/v - tris chloride BCC 0.1 M pH 8 Sodium fluoride 0.2 M - 🔍	pH 8	360 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of 1,4-Dioxane (50 v/v) 250 μl of Sodium fluoride (0.8 M) 100 μl of tris chloride BCC pH 8 (1 M) 190 μl of water (100 v/v)
F3	Polyethylene glycol 8,000 25 w/v - Jeffamine® M-600 5 v/v - MES 0.09 M pH 6 Magnesium acetate 0.09 M - 🔍	pH 6	500 μl of Polyethylene glycol 8,000 (50 w/v) 45 μl of Magnesium acetate (2 M) 30 μl of MES pH 6 (3 M) 100 μl of Jeffamine® M-600 (50 v/v) 325 μl of water (100 v/v)
F4	Polyethylene glycol 8,000 25 w/v - tris chloride BCC 0.1 M pH 8.5 Magnesium sulfate 0.2 M - 🔍	pH 8.5	100 μl of Magnesium sulfate (2 M) 500 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 300 μl of water (100 v/v)
F5	Polyethylene glycol 10,000 5 w/v - HEPES 0.1 M pH 7.5 Magnesium chloride 0.2 M - 🔍	pH 7.5	80 μl of Magnesium chloride pH 7 (2.5 M) 100 μl of HEPES pH 7.5 (1 M) 100 μl of Polyethylene glycol 10,000 (50 w/v) 720 μl of water (100 v/v)
F6	Polyethylene glycol 10,000 10 w/v - Sodium acetate 0.05 M pH 5 🔍	pH 5	16 μl of Sodium acetate pH 5 (3 M) 200 μl of Polyethylene glycol 10,000 (50 w/v) 783 μl of water (100 v/v)
F7	Polyethylene glycol 10,000 10 w/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 200 μl of Polyethylene glycol 10,000 (50 w/v) 700 μl of water (100 v/v)
F8	Polyethylene glycol 10,000 15 w/v - BIS-TRIS 0.1 M pH 5.5 Ammonium acetate 0.1 M - n-Dodecyl-N,N-dimethylamine-N-oxide 5 mM - 🔍	pH 5.5	20 μl of Ammonium acetate (5 M) 100 μl of BIS-TRIS pH 5.5 (1 M) 50 μl of n-Dodecyl-N,N-dimethylamine-N-oxide (100 mM) 300 μl of Polyethylene glycol 10,000 (50 w/v) 530 μl of water (100 v/v)
F9	Polyethylene glycol 10,000 15 w/v - Glycine 0.06 M pH 9 tri-Sodium citrate 0.02 M - 🔍	pH 9	10 μl of tri-Sodium citrate (2 M) 300 μl of Polyethylene glycol 10,000 (50 w/v) 30 μl of Glycine pH 9 (2 M) 660 μl of water (100 v/v)
F10	Polyethylene glycol 10,000 20 w/v - Polyethylene glycol monomethyl ether 550 5 v/v - tri-Sodium citrate 0.1 M pH 5.6 🔍	pH 5.6	100 μl of tri-Sodium citrate pH 5.6 (1 M) 400 μl of Polyethylene glycol 10,000 (50 w/v) 50 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 450 μl of water (100 v/v)
F11	Polyethylene glycol 20,000 12 w/v - MES 0.1 M pH 6.5 🔍	pH 6.5	240 μl of Polyethylene glycol 20,000 (50 w/v) 100 μl of MES pH 6.5 (1 M) 660 μl of water (100 v/v)
F12	Polyethylene glycol 20,000 17 w/v - tris chloride BCC 0.1 M pH 8.5 Magnesium chloride 0.1 M - 🔍	pH 8.5	40 μl of Magnesium chloride pH 7 (2.5 M) 340 μl of Polyethylene glycol 20,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 520 μl of water (100 v/v)
G1	Ammonium sulfate 0.5 M - HEPES 0.1 M pH 7.5 Lithium sulfate 0.2 M - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 166 μl of Ammonium sulfate (3 M) 66 μl of Lithium sulfate (3 M) 666 μl of water (100 v/v)
G2	Ammonium sulfate 1 M - MES 0.1 M pH 6.5 Potassium chloride 0.1 M - 🔍	pH 6.5	333 μl of Ammonium sulfate (3 M) 20 μl of Potassium chloride (5 M) 100 μl of MES pH 6.5 (1 M) 546 μl of water (100 v/v)
G3	Ammonium sulfate 1 M - Potassium chloride 0.8 M - HEPES 0.1 M pH 7 🔍	pH 7	333 μl of Ammonium sulfate (3 M) 160 μl of Potassium chloride (5 M) 100 μl of HEPES pH 7 (1 M) 406 μl of water (100 v/v)
G4	Ammonium sulfate 1.3 M - CHES 0.1 M pH 9.5 Sodium chloride 0.2 M - 🔍	pH 9.5	433 μl of Ammonium sulfate (3 M) 100 μl of CHES pH 9.5 (1 M) 200 μl of Sodium chloride (1 M) 266 μl of water (100 v/v)
G5	Ammonium sulfate 1.3 M - Sodium acetate 0.1 M pH 5.5 🔍	pH 5.5	33 μl of Sodium acetate pH 5.5 (3 M) 433 μl of Ammonium sulfate (3 M) 533 μl of water (100 v/v)
G6	Ammonium sulfate 1.3 M - Glycerol 5 v/v - di-Sodium maleate 0.1 M pH 6.5 Magnesium chloride 0.001 M - Zinc chloride 0.005 M - 🔍	pH 6.5	50 μl of Glycerol (100 v/v) 100 μl of di-Sodium maleate pH 6.5 (1 M) 0 μl of Magnesium chloride pH 7 (2.5 M) 433 μl of Ammonium sulfate (3 M) 5 μl of Zinc chloride (1 M) 411 μl of water (100 v/v)
G7	Ammonium sulfate 1.5 M - tris chloride BCC 0.1 M pH 8.5 Lithium sulfate 0.2 M - 🔍	pH 8.5	500 μl of Ammonium sulfate (3 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 66 μl of Lithium sulfate (3 M) 333 μl of water (100 v/v)
G8	Ammonium sulfate 1.7 M - MES 0.1 M pH 6 🔍	pH 6	33 μl of MES pH 6 (3 M) 566 μl of Ammonium sulfate (3 M) 400 μl of water (100 v/v)
G9	Ammonium sulfate 1.7 M - 2-methyl-2,4-pentanediol BCC 4 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 566 μl of Ammonium sulfate (3 M) 40 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 293 μl of water (100 v/v)
G10	Ammonium sulfate 1.7 M - Polyethylene glycol 400 2 v/v - tris chloride BCC 0.1 M pH 7.5 🔍	pH 7.5	33 μl of tris chloride BCC pH 7.5 (3 M) 20 μl of Polyethylene glycol 400 (100 v/v) 566 μl of Ammonium sulfate (3 M) 380 μl of water (100 v/v)
G11	Ammonium sulfate 2 M - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	666 μl of Ammonium sulfate (3 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 233 μl of water (100 v/v)
G12	Ammonium sulfate 2 M - Polyethylene glycol 400 10 v/v - HEPES 0.05 M pH 7.5 Zinc sulfate 0.05 M - 🔍	pH 7.5	100 μl of Polyethylene glycol 400 (100 v/v) 50 μl of Zinc sulfate (1 M) 50 μl of HEPES pH 7.5 (1 M) 666 μl of Ammonium sulfate (3 M) 133 μl of water (100 v/v)
H1	Ammonium sulfate 2 M - tri-Sodium citrate 0.1 M pH 5.6 Potassium Sodium tartrate 0.2 M - 🔍	pH 5.6	100 μl of Potassium Sodium tartrate (2 M) 666 μl of Ammonium sulfate (3 M) 100 μl of tri-Sodium citrate pH 5.6 (1 M) 133 μl of water (100 v/v)
H2	Ammonium sulfate 2.2 M - Polypropylene glycol 400 6 v/v - BIS-TRIS propane 0.1 M pH 6.5 🔍	pH 6.5	60 μl of Polypropylene glycol 400 (100 v/v) 733 μl of Ammonium sulfate (3 M) 100 μl of BIS-TRIS propane pH 6.5 (1 M) 106 μl of water (100 v/v)
H3	Ammonium sulfate 2.4 M - HEPES 0.1 M pH 7 🔍	pH 7	800 μl of Ammonium sulfate (3 M) 100 μl of HEPES pH 7 (1 M) 100 μl of water (100 v/v)
H4	Ammonium sulfate 2.4 M - tris chloride BCC 0.1 M pH 8 Magnesium chloride 0.01 M - Zinc sulfate 0.005 M - 🔍	pH 8	10 μl of Magnesium chloride pH 7 (1 M) 5 μl of Zinc sulfate (1 M) 800 μl of Ammonium sulfate (3 M) 100 μl of tris chloride BCC pH 8 (1 M) 85 μl of water (100 v/v)
H5	Lithium sulfate 0.5 M - Polyethylene glycol 400 10 v/v - Imidazole 0.1 M pH 8 🔍	pH 8	100 μl of Imidazole pH 8 (1 M) 100 μl of Polyethylene glycol 400 (100 v/v) 166 μl of Lithium sulfate (3 M) 633 μl of water (100 v/v)
H6	Lithium sulfate 1.5 M - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 500 μl of Lithium sulfate (3 M) 400 μl of water (100 v/v)
H7	Lithium sulfate 2 M - Polyethylene glycol 400 2 v/v - TRIS-Acetate 0.1 M pH 8 ß-Mercaptoethanol 0.1 v/v - 🔍	pH 8	20 μl of Polyethylene glycol 400 (100 v/v) 50 μl of ß-Mercaptoethanol (2 v/v) 50 μl of TRIS-Acetate pH 8 (2 M) 666 μl of Lithium sulfate (3 M) 213 μl of water (100 v/v)
H8	2-methyl-2,4-pentanediol BCC 20 v/v - MES 0.1 M pH 6.5 🔍	pH 6.5	100 μl of MES pH 6.5 (1 M) 200 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 700 μl of water (100 v/v)
H9	2-methyl-2,4-pentanediol BCC 35 v/v - Sodium acetate 0.1 M pH 4.6 🔍	pH 4.6	350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100 μl of Sodium acetate pH 4.6 (1 M) 550 μl of water (100 v/v)
H10	Sodium formate 1.4 M - MES 0.1 M pH 6 🔍	pH 6	200 μl of Sodium formate (7 M) 33 μl of MES pH 6 (3 M) 766 μl of water (100 v/v)
H11	Sodium formate 3 M - tris chloride BCC 0.1 M pH 8 🔍	pH 8	428 μl of Sodium formate (7 M) 100 μl of tris chloride BCC pH 8 (1 M) 471 μl of water (100 v/v)
H12	di-Sodium tartrate 1 M - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	100 μl of tris chloride BCC pH 8.5 (1 M) 500 μl of di-Sodium tartrate (2 M) 400 μl of water (100 v/v)