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Supplemental Information

Screen Description

Index_C3

BCC Core Screens / Index_C3
Created 2025-03-07.
Based on Hampton's Index screen, made up in C3.

Export

Barcodes

Index_C3

  • Z0002572
  • Z0002573

Screen Structure

Index_C3

Well Count 96
Number of distinct ingredients 49
Internal diversity (score mean dist) 0.910

Screen Composition

2025-06-14T13:50:22.131919 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
ingredient type Index_C3
Buffer 91
Complex ingredient 4
Organic (non-volatile) 8
Polymer 60
Salt 88

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Index_C3 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • ammonium sulfate BCC 2 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 3.5
pH 3.5
30 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
70 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
571 μl of ammonium sulfate BCC (3.5 M)
328 μl of water (100 v/v)
A2
  • ammonium sulfate BCC 2 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
571 μl of ammonium sulfate BCC (3.5 M)
31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
328 μl of water (100 v/v)
A3
  • ammonium sulfate BCC 2 M -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
100 μl of bis-tris chloride BCC pH 5.5 (1 M)
571 μl of ammonium sulfate BCC (3.5 M)
328 μl of water (100 v/v)
A4
  • ammonium sulfate BCC 2 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
571 μl of ammonium sulfate BCC (3.5 M)
328 μl of water (100 v/v)
A5
  • ammonium sulfate BCC 2 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
571 μl of ammonium sulfate BCC (3.5 M)
328 μl of water (100 v/v)
A6
  • ammonium sulfate BCC 2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
78 μl of tris chloride BCC pH 9 (1 M)
571 μl of ammonium sulfate BCC (3.5 M)
21 μl of tris chloride BCC pH 7 (1 M)
328 μl of water (100 v/v)
A7
  • sodium chloride BCC 3 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 3.5
pH 3.5
600 μl of sodium chloride BCC (5 M)
30 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
70 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
300 μl of water (100 v/v)
A8
  • sodium chloride BCC 3 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
600 μl of sodium chloride BCC (5 M)
68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
300 μl of water (100 v/v)
A9
  • sodium chloride BCC 3 M -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
600 μl of sodium chloride BCC (5 M)
100 μl of bis-tris chloride BCC pH 5.5 (1 M)
300 μl of water (100 v/v)
A10
  • sodium chloride BCC 3 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
600 μl of sodium chloride BCC (5 M)
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
300 μl of water (100 v/v)
A11
  • sodium chloride BCC 3 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
600 μl of sodium chloride BCC (5 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
300 μl of water (100 v/v)
A12
  • sodium chloride BCC 3 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
600 μl of sodium chloride BCC (5 M)
78 μl of tris chloride BCC pH 9 (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
300 μl of water (100 v/v)
B1
  • magnesium formate BCC 0.3 M -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
300 μl of magnesium formate BCC (1 M)
100 μl of bis-tris chloride BCC pH 5.5 (1 M)
600 μl of water (100 v/v)
B2
  • magnesium formate BCC 0.5 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
500 μl of magnesium formate BCC (1 M)
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
400 μl of water (100 v/v)
B3
  • magnesium formate BCC 0.5 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
500 μl of magnesium formate BCC (1 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
400 μl of water (100 v/v)
B4
  • magnesium formate BCC 0.3 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
300 μl of magnesium formate BCC (1 M)
78 μl of tris chloride BCC pH 9 (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
600 μl of water (100 v/v)
B5
  • dipotassium hydrogen phosphate BCC 0.14 M -
  • sodium dihydrogen phosphate BCC 1.26 M -
NA
420 μl of sodium dihydrogen phosphate BCC (3 M)
70 μl of dipotassium hydrogen phosphate BCC (2 M)
510 μl of water (100 v/v)
B6
  • dipotassium hydrogen phosphate BCC 0.91 M -
  • sodium dihydrogen phosphate BCC 0.49 M -
NA
163 μl of sodium dihydrogen phosphate BCC (3 M)
455 μl of dipotassium hydrogen phosphate BCC (2 M)
381 μl of water (100 v/v)
B7
  • dipotassium hydrogen phosphate BCC 1.34 M -
  • sodium dihydrogen phosphate BCC 0.056 M -
NA
18 μl of sodium dihydrogen phosphate BCC (3 M)
672 μl of dipotassium hydrogen phosphate BCC (2 M)
309 μl of water (100 v/v)
B8
  • sodium HEPES BCC 0.1 M pH 7.5
  • trisodium citrate BCC 1.4 M -
pH 7.5
875 μl of trisodium citrate BCC (1.6 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
25 μl of water (100 v/v)
B9
  • triammonium citrate BCC 1.8 M -
NA
720 μl of triammonium citrate BCC (2.5 M)
280 μl of water (100 v/v)
B10
  • sodium succinate BCC 0.8 M -
NA
533 μl of sodium succinate BCC (1.5 M)
466 μl of water (100 v/v)
B11
  • sodium DL-malate BCC 2.1 M pH 7
pH 7
840 μl of sodium DL-malate BCC pH 7 (2.5 M)
160 μl of water (100 v/v)
B12
  • sodium acetate BCC 2.8 M -
NA
933 μl of sodium acetate BCC (3 M)
66 μl of water (100 v/v)
C1
  • sodium formate BCC 3.5 M -
NA
875 μl of sodium formate BCC (4 M)
125 μl of water (100 v/v)
C2
  • diammonium tartrate BCC 1.1 M -
NA
550 μl of diammonium tartrate BCC (2 M)
450 μl of water (100 v/v)
C3
  • sodium malonate-malonic acid BCC 2.4 M pH 7
pH 7
705 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
294 μl of water (100 v/v)
C4
  • tacsimate BCC 35 v/v pH 7
pH 7
350 μl of tacsimate BCC pH 7 (100 v/v)
650 μl of water (100 v/v)
C5
  • tacsimate BCC 60 v/v pH 7
pH 7
600 μl of tacsimate BCC pH 7 (100 v/v)
400 μl of water (100 v/v)
C6
  • ammonium sulfate BCC 1.5 M -
  • sodium chloride BCC 0.1 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
20 μl of sodium chloride BCC (5 M)
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
428 μl of ammonium sulfate BCC (3.5 M)
451 μl of water (100 v/v)
C7
  • polyethylene glycol monomethyl ether 5000 BCC 0.5 w/v -
  • potassium sodium tartrate BCC 0.8 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
400 μl of potassium sodium tartrate BCC (2 M)
10 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
78 μl of tris chloride BCC pH 9 (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
490 μl of water (100 v/v)
C8
  • ammonium sulfate BCC 1 M -
  • polyethylene glycol 3350 BCC 1 w/v -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
20 μl of polyethylene glycol 3350 BCC (50 w/v)
100 μl of bis-tris chloride BCC pH 5.5 (1 M)
285 μl of ammonium sulfate BCC (3.5 M)
594 μl of water (100 v/v)
C9
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 0.5 w/v -
  • sodium malonate-malonic acid BCC 1.1 M pH 7
NA
81 μl of sodium HEPES BCC pH 6.5 (1 M)
18 μl of sodium HEPES BCC pH 8.5 (1 M)
323 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
10 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
566 μl of water (100 v/v)
C10
  • sodium HEPES BCC 0.1 M pH 7
  • sodium succinate BCC 1 M -
  • polyethylene glycol monomethyl ether 2000 BCC 1 w/v -
pH 7
81 μl of sodium HEPES BCC pH 6.5 (1 M)
18 μl of sodium HEPES BCC pH 8.5 (1 M)
666 μl of sodium succinate BCC (1.5 M)
20 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
213 μl of water (100 v/v)
C11
  • ammonium sulfate BCC 1 M -
  • sodium HEPES BCC 0.1 M pH 7
  • polyethylene glycol 8000 BCC 0.5 w/v -
pH 7
81 μl of sodium HEPES BCC pH 6.5 (1 M)
18 μl of sodium HEPES BCC pH 8.5 (1 M)
285 μl of ammonium sulfate BCC (3.5 M)
25 μl of polyethylene glycol 8000 BCC (20 w/v)
589 μl of water (100 v/v)
C12
  • polyethylene glycol 3350 BCC 2 w/v -
  • sodium HEPES BCC 0.1 M pH 7
  • tacsimate BCC 15 v/v pH 7
NA
81 μl of sodium HEPES BCC pH 6.5 (1 M)
18 μl of sodium HEPES BCC pH 8.5 (1 M)
150 μl of tacsimate BCC pH 7 (100 v/v)
40 μl of polyethylene glycol 3350 BCC (50 w/v)
710 μl of water (100 v/v)
D1
  • polyethylene glycol 1500 BCC 25 w/v -
NA
500 μl of polyethylene glycol 1500 BCC (50 w/v)
500 μl of water (100 v/v)
D2
  • jeffamine M-600 BCC 30 v/v -
  • sodium HEPES BCC 0.1 M pH 7
pH 7
81 μl of sodium HEPES BCC pH 6.5 (1 M)
18 μl of sodium HEPES BCC pH 8.5 (1 M)
600 μl of jeffamine M-600 BCC pH 7 (50 v/v)
300 μl of water (100 v/v)
D3
  • sodium HEPES BCC 0.1 M pH 7
  • jeffamine ED-2001 BCC 30 w/v -
pH 7
81 μl of sodium HEPES BCC pH 6.5 (1 M)
18 μl of sodium HEPES BCC pH 8.5 (1 M)
600 μl of jeffamine ED-2001 BCC pH 7 (50 w/v)
300 μl of water (100 v/v)
D4
  • polyethylene glycol 3350 BCC 25 w/v -
  • trisodium citrate-citric acid BCC 0.1 M pH 3.5
pH 3.5
30 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
500 μl of polyethylene glycol 3350 BCC (50 w/v)
70 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400 μl of water (100 v/v)
D5
  • polyethylene glycol 3350 BCC 25 w/v -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
500 μl of polyethylene glycol 3350 BCC (50 w/v)
68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
400 μl of water (100 v/v)
D6
  • polyethylene glycol 3350 BCC 25 w/v -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
500 μl of polyethylene glycol 3350 BCC (50 w/v)
100 μl of bis-tris chloride BCC pH 5.5 (1 M)
400 μl of water (100 v/v)
D7
  • polyethylene glycol 3350 BCC 25 w/v -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
500 μl of polyethylene glycol 3350 BCC (50 w/v)
400 μl of water (100 v/v)
D8
  • polyethylene glycol 3350 BCC 25 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
500 μl of polyethylene glycol 3350 BCC (50 w/v)
400 μl of water (100 v/v)
D9
  • polyethylene glycol 3350 BCC 25 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
78 μl of tris chloride BCC pH 9 (1 M)
500 μl of polyethylene glycol 3350 BCC (50 w/v)
21 μl of tris chloride BCC pH 7 (1 M)
400 μl of water (100 v/v)
D10
  • polyethylene glycol monomethyl ether 5000 BCC 20 w/v -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
400 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
500 μl of water (100 v/v)
D11
  • polyethylene glycol monomethyl ether 2000 BCC 28 w/v -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
560 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
340 μl of water (100 v/v)
D12
  • 2-methyl-2,4-pentanediol BCC 45 v/v -
  • calcium chloride BCC 0.2 M -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
200 μl of calcium chloride BCC (1 M)
450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100 μl of bis-tris chloride BCC pH 5.5 (1 M)
250 μl of water (100 v/v)
E1
  • 2-methyl-2,4-pentanediol BCC 45 v/v -
  • calcium chloride BCC 0.2 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
200 μl of calcium chloride BCC (1 M)
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
250 μl of water (100 v/v)
E2
  • 2-methyl-2,4-pentanediol BCC 45 v/v -
  • ammonium acetate BCC 0.2 M -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
40 μl of ammonium acetate BCC (5 M)
450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100 μl of bis-tris chloride BCC pH 5.5 (1 M)
410 μl of water (100 v/v)
E3
  • 2-methyl-2,4-pentanediol BCC 45 v/v -
  • ammonium acetate BCC 0.2 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
40 μl of ammonium acetate BCC (5 M)
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
410 μl of water (100 v/v)
E4
  • 2-methyl-2,4-pentanediol BCC 45 v/v -
  • ammonium acetate BCC 0.2 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
40 μl of ammonium acetate BCC (5 M)
450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
410 μl of water (100 v/v)
E5
  • 2-methyl-2,4-pentanediol BCC 45 v/v -
  • ammonium acetate BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
40 μl of ammonium acetate BCC (5 M)
78 μl of tris chloride BCC pH 9 (1 M)
450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
21 μl of tris chloride BCC pH 7 (1 M)
410 μl of water (100 v/v)
E6
  • calcium chloride BCC 0.05 M -
  • polyethylene glycol monomethyl ether 550 BCC 30 v/v -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
50 μl of calcium chloride BCC (1 M)
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
352 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
497 μl of water (100 v/v)
E7
  • magnesium chloride BCC 0.05 M -
  • polyethylene glycol monomethyl ether 550 BCC 30 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
50 μl of magnesium chloride BCC (1 M)
352 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
497 μl of water (100 v/v)
E8
  • potassium chloride BCC 0.2 M -
  • sodium HEPES BCC 0.05 M pH 7.5
  • pentaerythritol propoxylate (5/4 PO/OH) BCC 35 w/v -
pH 7.5
25 μl of sodium HEPES BCC pH 6.5 (1 M)
25 μl of sodium HEPES BCC pH 8.5 (1 M)
200 μl of potassium chloride BCC (1 M)
700 μl of pentaerythritol propoxylate (5/4 PO/OH) BCC (50 w/v)
50 μl of water (100 v/v)
E9
  • ammonium sulfate BCC 0.05 M -
  • pentaerythritol ethoxylate (15/4 EO/OH) BCC 30 w/v -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
600 μl of pentaerythritol ethoxylate (15/4 EO/OH) BCC (50 w/v)
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
14 μl of ammonium sulfate BCC (3.5 M)
285 μl of water (100 v/v)
E10
  • polypropylene glycol P400 BCC 45 v/v -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
750 μl of polypropylene glycol P400 BCC (60 v/v)
150 μl of water (100 v/v)
E11
  • magnesium chloride BCC 0.02 M -
  • sodium HEPES BCC 0.1 M pH 7.5
  • polyacrylic acid 5100 sodium salt BCC 22 w/v -
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
20 μl of magnesium chloride BCC (1 M)
733 μl of polyacrylic acid 5100 sodium salt BCC (30 w/v)
146 μl of water (100 v/v)
E12
  • cobalt chloride BCC 0.01 M -
  • tris chloride BCC 0.1 M pH 8.5
  • polyvinylpyrrolidone K15 BCC 20 w/v -
pH 8.5
100 μl of cobalt chloride BCC (0.1 M)
78 μl of tris chloride BCC pH 9 (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
400 μl of polyvinylpyrrolidone K15 BCC (50 w/v)
400 μl of water (100 v/v)
F1
  • polyethylene glycol 3350 BCC 10 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
  • proline BCC 0.2 M -
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
200 μl of proline BCC (1 M)
200 μl of polyethylene glycol 3350 BCC (50 w/v)
500 μl of water (100 v/v)
F2
  • tris chloride BCC 0.1 M pH 8.5
  • polyethylene glycol monomethyl ether 2000 BCC 20 w/v -
  • trimethylamine N-oxide BCC 0.2 M -
pH 8.5
78 μl of tris chloride BCC pH 9 (1 M)
400 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
200 μl of trimethylamine N-oxide BCC (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
300 μl of water (100 v/v)
F3
  • polyethylene glycol monomethyl ether 5000 BCC 10 w/v -
  • sodium HEPES BCC 0.1 M pH 7
  • tacsimate BCC 5 v/v pH 7
NA
81 μl of sodium HEPES BCC pH 6.5 (1 M)
18 μl of sodium HEPES BCC pH 8.5 (1 M)
200 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v)
50 μl of tacsimate BCC pH 7 (100 v/v)
650 μl of water (100 v/v)
F4
  • cadmium chloride BCC 0.005 M -
  • cobalt chloride BCC 0.005 M -
  • magnesium chloride BCC 0.005 M -
  • nickel(II) chloride BCC 0.005 M -
  • polyethylene glycol 3350 BCC 12 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of cadmium chloride BCC (0.1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
5 μl of magnesium chloride BCC (1 M)
50 μl of cobalt chloride BCC (0.1 M)
5 μl of nickel(II) chloride BCC (1 M)
240 μl of polyethylene glycol 3350 BCC (50 w/v)
550 μl of water (100 v/v)
F5
  • ammonium acetate BCC 0.1 M -
  • polyethylene glycol 10000 BCC 17 w/v -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
20 μl of ammonium acetate BCC (5 M)
100 μl of bis-tris chloride BCC pH 5.5 (1 M)
566 μl of polyethylene glycol 10000 BCC (30 w/v)
313 μl of water (100 v/v)
F6
  • ammonium sulfate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
500 μl of polyethylene glycol 3350 BCC (50 w/v)
100 μl of bis-tris chloride BCC pH 5.5 (1 M)
57 μl of ammonium sulfate BCC (3.5 M)
342 μl of water (100 v/v)
F7
  • ammonium sulfate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
500 μl of polyethylene glycol 3350 BCC (50 w/v)
57 μl of ammonium sulfate BCC (3.5 M)
342 μl of water (100 v/v)
F8
  • ammonium sulfate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
500 μl of polyethylene glycol 3350 BCC (50 w/v)
57 μl of ammonium sulfate BCC (3.5 M)
342 μl of water (100 v/v)
F9
  • ammonium sulfate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
78 μl of tris chloride BCC pH 9 (1 M)
500 μl of polyethylene glycol 3350 BCC (50 w/v)
57 μl of ammonium sulfate BCC (3.5 M)
21 μl of tris chloride BCC pH 7 (1 M)
342 μl of water (100 v/v)
F10
  • polyethylene glycol 3350 BCC 25 w/v -
  • sodium chloride BCC 0.2 M -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
40 μl of sodium chloride BCC (5 M)
500 μl of polyethylene glycol 3350 BCC (50 w/v)
100 μl of bis-tris chloride BCC pH 5.5 (1 M)
360 μl of water (100 v/v)
F11
  • polyethylene glycol 3350 BCC 25 w/v -
  • sodium chloride BCC 0.2 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
40 μl of sodium chloride BCC (5 M)
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
500 μl of polyethylene glycol 3350 BCC (50 w/v)
360 μl of water (100 v/v)
F12
  • polyethylene glycol 3350 BCC 25 w/v -
  • sodium chloride BCC 0.2 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
40 μl of sodium chloride BCC (5 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
500 μl of polyethylene glycol 3350 BCC (50 w/v)
360 μl of water (100 v/v)
G1
  • polyethylene glycol 3350 BCC 25 w/v -
  • sodium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
40 μl of sodium chloride BCC (5 M)
78 μl of tris chloride BCC pH 9 (1 M)
500 μl of polyethylene glycol 3350 BCC (50 w/v)
21 μl of tris chloride BCC pH 7 (1 M)
360 μl of water (100 v/v)
G2
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
200 μl of lithium sulfate BCC (1 M)
500 μl of polyethylene glycol 3350 BCC (50 w/v)
100 μl of bis-tris chloride BCC pH 5.5 (1 M)
200 μl of water (100 v/v)
G3
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
200 μl of lithium sulfate BCC (1 M)
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
500 μl of polyethylene glycol 3350 BCC (50 w/v)
200 μl of water (100 v/v)
G4
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
200 μl of lithium sulfate BCC (1 M)
500 μl of polyethylene glycol 3350 BCC (50 w/v)
200 μl of water (100 v/v)
G5
  • lithium sulfate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
78 μl of tris chloride BCC pH 9 (1 M)
200 μl of lithium sulfate BCC (1 M)
500 μl of polyethylene glycol 3350 BCC (50 w/v)
21 μl of tris chloride BCC pH 7 (1 M)
200 μl of water (100 v/v)
G6
  • ammonium acetate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
40 μl of ammonium acetate BCC (5 M)
500 μl of polyethylene glycol 3350 BCC (50 w/v)
100 μl of bis-tris chloride BCC pH 5.5 (1 M)
360 μl of water (100 v/v)
G7
  • ammonium acetate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
40 μl of ammonium acetate BCC (5 M)
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
500 μl of polyethylene glycol 3350 BCC (50 w/v)
360 μl of water (100 v/v)
G8
  • ammonium acetate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
40 μl of ammonium acetate BCC (5 M)
500 μl of polyethylene glycol 3350 BCC (50 w/v)
360 μl of water (100 v/v)
G9
  • ammonium acetate BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
40 μl of ammonium acetate BCC (5 M)
78 μl of tris chloride BCC pH 9 (1 M)
500 μl of polyethylene glycol 3350 BCC (50 w/v)
21 μl of tris chloride BCC pH 7 (1 M)
360 μl of water (100 v/v)
G10
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
200 μl of magnesium chloride BCC (1 M)
500 μl of polyethylene glycol 3350 BCC (50 w/v)
100 μl of bis-tris chloride BCC pH 5.5 (1 M)
200 μl of water (100 v/v)
G11
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
200 μl of magnesium chloride BCC (1 M)
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
500 μl of polyethylene glycol 3350 BCC (50 w/v)
200 μl of water (100 v/v)
G12
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
200 μl of magnesium chloride BCC (1 M)
500 μl of polyethylene glycol 3350 BCC (50 w/v)
200 μl of water (100 v/v)
H1
  • magnesium chloride BCC 0.2 M -
  • polyethylene glycol 3350 BCC 25 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
200 μl of magnesium chloride BCC (1 M)
78 μl of tris chloride BCC pH 9 (1 M)
500 μl of polyethylene glycol 3350 BCC (50 w/v)
21 μl of tris chloride BCC pH 7 (1 M)
200 μl of water (100 v/v)
H2
  • polyethylene glycol 3350 BCC 20 w/v -
  • potassium sodium tartrate BCC 0.2 M -
NA
100 μl of potassium sodium tartrate BCC (2 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
500 μl of water (100 v/v)
H3
  • polyethylene glycol 3350 BCC 20 w/v -
  • sodium malonate-malonic acid BCC 0.2 M pH 7
pH 7
400 μl of polyethylene glycol 3350 BCC (50 w/v)
58 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M)
541 μl of water (100 v/v)
H4
  • polyethylene glycol 3350 BCC 20 w/v -
  • triammonium citrate BCC 0.2 M -
NA
80 μl of triammonium citrate BCC (2.5 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
520 μl of water (100 v/v)
H5
  • polyethylene glycol 3350 BCC 15 w/v -
  • sodium succinate BCC 0.1 M -
NA
66 μl of sodium succinate BCC (1.5 M)
300 μl of polyethylene glycol 3350 BCC (50 w/v)
633 μl of water (100 v/v)
H6
  • polyethylene glycol 3350 BCC 20 w/v -
  • sodium formate BCC 0.2 M -
NA
400 μl of polyethylene glycol 3350 BCC (50 w/v)
50 μl of sodium formate BCC (4 M)
550 μl of water (100 v/v)
H7
  • polyethylene glycol 3350 BCC 20 w/v -
  • DL-malic acid BCC 0.15 M pH 7
pH 7
150 μl of DL-malic acid BCC pH 7 (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
450 μl of water (100 v/v)
H8
  • magnesium formate BCC 0.1 M -
  • polyethylene glycol 3350 BCC 15 w/v -
NA
100 μl of magnesium formate BCC (1 M)
300 μl of polyethylene glycol 3350 BCC (50 w/v)
600 μl of water (100 v/v)
H9
  • polyethylene glycol 3350 BCC 20 w/v -
  • zinc acetate BCC 0.05 M -
NA
50 μl of zinc acetate BCC (1 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
550 μl of water (100 v/v)
H10
  • polyethylene glycol 3350 BCC 20 w/v -
  • trisodium citrate BCC 0.2 M -
NA
125 μl of trisodium citrate BCC (1.6 M)
400 μl of polyethylene glycol 3350 BCC (50 w/v)
475 μl of water (100 v/v)
H11
  • potassium thiocyanate BCC 0.1 M -
  • polyethylene glycol monomethyl ether 2000 BCC 30 w/v -
NA
600 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
50 μl of potassium thiocyanate BCC (2 M)
350 μl of water (100 v/v)
H12
  • potassium bromide BCC 0.15 M -
  • polyethylene glycol monomethyl ether 2000 BCC 30 w/v -
NA
150 μl of potassium bromide BCC (1 M)
600 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v)
250 μl of water (100 v/v)