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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
Index_C3
BCC Core Screens / Index_C3
Created 2025-03-07.
Based on Hampton's Index screen, made up in C3.
Export
Index_C3
Barcodes
Index_C3
- Z0002572
- Z0002573
Screen Structure
Index_C3
Well Count | 96 |
---|---|
Number of distinct ingredients | 49 |
Internal diversity (score mean dist) | 0.910 |
Screen Composition
ingredient type | Index_C3 |
---|---|
Buffer | 91 |
Complex ingredient | 4 |
Organic (non-volatile) | 8 |
Polymer | 60 |
Salt | 88 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 3.5 | 30 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 70 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 571 μl of ammonium sulfate BCC (3.5 M) 328 μl of water (100 v/v) |
A2 |
| pH 4.5 | 68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 571 μl of ammonium sulfate BCC (3.5 M) 31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 328 μl of water (100 v/v) |
A3 |
| pH 5.5 | 100 μl of bis-tris chloride BCC pH 5.5 (1 M) 571 μl of ammonium sulfate BCC (3.5 M) 328 μl of water (100 v/v) |
A4 |
| pH 6.5 | 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 571 μl of ammonium sulfate BCC (3.5 M) 328 μl of water (100 v/v) |
A5 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 571 μl of ammonium sulfate BCC (3.5 M) 328 μl of water (100 v/v) |
A6 |
| pH 8.5 | 78 μl of tris chloride BCC pH 9 (1 M) 571 μl of ammonium sulfate BCC (3.5 M) 21 μl of tris chloride BCC pH 7 (1 M) 328 μl of water (100 v/v) |
A7 |
| pH 3.5 | 600 μl of sodium chloride BCC (5 M) 30 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 70 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 300 μl of water (100 v/v) |
A8 |
| pH 4.5 | 600 μl of sodium chloride BCC (5 M) 68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 300 μl of water (100 v/v) |
A9 |
| pH 5.5 | 600 μl of sodium chloride BCC (5 M) 100 μl of bis-tris chloride BCC pH 5.5 (1 M) 300 μl of water (100 v/v) |
A10 |
| pH 6.5 | 600 μl of sodium chloride BCC (5 M) 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 300 μl of water (100 v/v) |
A11 |
| pH 7.5 | 600 μl of sodium chloride BCC (5 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 300 μl of water (100 v/v) |
A12 |
| pH 8.5 | 600 μl of sodium chloride BCC (5 M) 78 μl of tris chloride BCC pH 9 (1 M) 21 μl of tris chloride BCC pH 7 (1 M) 300 μl of water (100 v/v) |
B1 |
| pH 5.5 | 300 μl of magnesium formate BCC (1 M) 100 μl of bis-tris chloride BCC pH 5.5 (1 M) 600 μl of water (100 v/v) |
B2 |
| pH 6.5 | 500 μl of magnesium formate BCC (1 M) 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 400 μl of water (100 v/v) |
B3 |
| pH 7.5 | 500 μl of magnesium formate BCC (1 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 400 μl of water (100 v/v) |
B4 |
| pH 8.5 | 300 μl of magnesium formate BCC (1 M) 78 μl of tris chloride BCC pH 9 (1 M) 21 μl of tris chloride BCC pH 7 (1 M) 600 μl of water (100 v/v) |
B5 |
| NA | 420 μl of sodium dihydrogen phosphate BCC (3 M) 70 μl of dipotassium hydrogen phosphate BCC (2 M) 510 μl of water (100 v/v) |
B6 |
| NA | 163 μl of sodium dihydrogen phosphate BCC (3 M) 455 μl of dipotassium hydrogen phosphate BCC (2 M) 381 μl of water (100 v/v) |
B7 |
| NA | 18 μl of sodium dihydrogen phosphate BCC (3 M) 672 μl of dipotassium hydrogen phosphate BCC (2 M) 309 μl of water (100 v/v) |
B8 |
| pH 7.5 | 875 μl of trisodium citrate BCC (1.6 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 25 μl of water (100 v/v) |
B9 |
| NA | 720 μl of triammonium citrate BCC (2.5 M) 280 μl of water (100 v/v) |
B10 |
| NA | 533 μl of sodium succinate BCC (1.5 M) 466 μl of water (100 v/v) |
B11 |
| pH 7 | 840 μl of sodium DL-malate BCC pH 7 (2.5 M) 160 μl of water (100 v/v) |
B12 |
| NA | 933 μl of sodium acetate BCC (3 M) 66 μl of water (100 v/v) |
C1 |
| NA | 875 μl of sodium formate BCC (4 M) 125 μl of water (100 v/v) |
C2 |
| NA | 550 μl of diammonium tartrate BCC (2 M) 450 μl of water (100 v/v) |
C3 |
| pH 7 | 705 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 294 μl of water (100 v/v) |
C4 |
| pH 7 | 350 μl of tacsimate BCC pH 7 (100 v/v) 650 μl of water (100 v/v) |
C5 |
| pH 7 | 600 μl of tacsimate BCC pH 7 (100 v/v) 400 μl of water (100 v/v) |
C6 |
| pH 6.5 | 20 μl of sodium chloride BCC (5 M) 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 428 μl of ammonium sulfate BCC (3.5 M) 451 μl of water (100 v/v) |
C7 |
| pH 8.5 | 400 μl of potassium sodium tartrate BCC (2 M) 10 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v) 78 μl of tris chloride BCC pH 9 (1 M) 21 μl of tris chloride BCC pH 7 (1 M) 490 μl of water (100 v/v) |
C8 |
| pH 5.5 | 20 μl of polyethylene glycol 3350 BCC (50 w/v) 100 μl of bis-tris chloride BCC pH 5.5 (1 M) 285 μl of ammonium sulfate BCC (3.5 M) 594 μl of water (100 v/v) |
C9 |
| NA | 81 μl of sodium HEPES BCC pH 6.5 (1 M) 18 μl of sodium HEPES BCC pH 8.5 (1 M) 323 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 10 μl of jeffamine ED-2001 BCC pH 7 (50 w/v) 566 μl of water (100 v/v) |
C10 |
| pH 7 | 81 μl of sodium HEPES BCC pH 6.5 (1 M) 18 μl of sodium HEPES BCC pH 8.5 (1 M) 666 μl of sodium succinate BCC (1.5 M) 20 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v) 213 μl of water (100 v/v) |
C11 |
| pH 7 | 81 μl of sodium HEPES BCC pH 6.5 (1 M) 18 μl of sodium HEPES BCC pH 8.5 (1 M) 285 μl of ammonium sulfate BCC (3.5 M) 25 μl of polyethylene glycol 8000 BCC (20 w/v) 589 μl of water (100 v/v) |
C12 |
| NA | 81 μl of sodium HEPES BCC pH 6.5 (1 M) 18 μl of sodium HEPES BCC pH 8.5 (1 M) 150 μl of tacsimate BCC pH 7 (100 v/v) 40 μl of polyethylene glycol 3350 BCC (50 w/v) 710 μl of water (100 v/v) |
D1 |
| NA | 500 μl of polyethylene glycol 1500 BCC (50 w/v) 500 μl of water (100 v/v) |
D2 |
| pH 7 | 81 μl of sodium HEPES BCC pH 6.5 (1 M) 18 μl of sodium HEPES BCC pH 8.5 (1 M) 600 μl of jeffamine M-600 BCC pH 7 (50 v/v) 300 μl of water (100 v/v) |
D3 |
| pH 7 | 81 μl of sodium HEPES BCC pH 6.5 (1 M) 18 μl of sodium HEPES BCC pH 8.5 (1 M) 600 μl of jeffamine ED-2001 BCC pH 7 (50 w/v) 300 μl of water (100 v/v) |
D4 |
| pH 3.5 | 30 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 70 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 400 μl of water (100 v/v) |
D5 |
| pH 4.5 | 500 μl of polyethylene glycol 3350 BCC (50 w/v) 68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 400 μl of water (100 v/v) |
D6 |
| pH 5.5 | 500 μl of polyethylene glycol 3350 BCC (50 w/v) 100 μl of bis-tris chloride BCC pH 5.5 (1 M) 400 μl of water (100 v/v) |
D7 |
| pH 6.5 | 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 400 μl of water (100 v/v) |
D8 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 400 μl of water (100 v/v) |
D9 |
| pH 8.5 | 78 μl of tris chloride BCC pH 9 (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 21 μl of tris chloride BCC pH 7 (1 M) 400 μl of water (100 v/v) |
D10 |
| pH 6.5 | 400 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v) 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 500 μl of water (100 v/v) |
D11 |
| pH 6.5 | 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 560 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v) 340 μl of water (100 v/v) |
D12 |
| pH 5.5 | 200 μl of calcium chloride BCC (1 M) 450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100 μl of bis-tris chloride BCC pH 5.5 (1 M) 250 μl of water (100 v/v) |
E1 |
| pH 6.5 | 200 μl of calcium chloride BCC (1 M) 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 250 μl of water (100 v/v) |
E2 |
| pH 5.5 | 40 μl of ammonium acetate BCC (5 M) 450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100 μl of bis-tris chloride BCC pH 5.5 (1 M) 410 μl of water (100 v/v) |
E3 |
| pH 6.5 | 40 μl of ammonium acetate BCC (5 M) 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 410 μl of water (100 v/v) |
E4 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 40 μl of ammonium acetate BCC (5 M) 450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 410 μl of water (100 v/v) |
E5 |
| pH 8.5 | 40 μl of ammonium acetate BCC (5 M) 78 μl of tris chloride BCC pH 9 (1 M) 450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 21 μl of tris chloride BCC pH 7 (1 M) 410 μl of water (100 v/v) |
E6 |
| pH 6.5 | 50 μl of calcium chloride BCC (1 M) 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 352 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v) 497 μl of water (100 v/v) |
E7 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 50 μl of magnesium chloride BCC (1 M) 352 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v) 497 μl of water (100 v/v) |
E8 |
| pH 7.5 | 25 μl of sodium HEPES BCC pH 6.5 (1 M) 25 μl of sodium HEPES BCC pH 8.5 (1 M) 200 μl of potassium chloride BCC (1 M) 700 μl of pentaerythritol propoxylate (5/4 PO/OH) BCC (50 w/v) 50 μl of water (100 v/v) |
E9 |
| pH 6.5 | 600 μl of pentaerythritol ethoxylate (15/4 EO/OH) BCC (50 w/v) 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 14 μl of ammonium sulfate BCC (3.5 M) 285 μl of water (100 v/v) |
E10 |
| pH 6.5 | 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 750 μl of polypropylene glycol P400 BCC (60 v/v) 150 μl of water (100 v/v) |
E11 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 20 μl of magnesium chloride BCC (1 M) 733 μl of polyacrylic acid 5100 sodium salt BCC (30 w/v) 146 μl of water (100 v/v) |
E12 |
| pH 8.5 | 100 μl of cobalt chloride BCC (0.1 M) 78 μl of tris chloride BCC pH 9 (1 M) 21 μl of tris chloride BCC pH 7 (1 M) 400 μl of polyvinylpyrrolidone K15 BCC (50 w/v) 400 μl of water (100 v/v) |
F1 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 200 μl of proline BCC (1 M) 200 μl of polyethylene glycol 3350 BCC (50 w/v) 500 μl of water (100 v/v) |
F2 |
| pH 8.5 | 78 μl of tris chloride BCC pH 9 (1 M) 400 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v) 200 μl of trimethylamine N-oxide BCC (1 M) 21 μl of tris chloride BCC pH 7 (1 M) 300 μl of water (100 v/v) |
F3 |
| NA | 81 μl of sodium HEPES BCC pH 6.5 (1 M) 18 μl of sodium HEPES BCC pH 8.5 (1 M) 200 μl of polyethylene glycol monomethyl ether 5000 BCC (50 w/v) 50 μl of tacsimate BCC pH 7 (100 v/v) 650 μl of water (100 v/v) |
F4 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of cadmium chloride BCC (0.1 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 5 μl of magnesium chloride BCC (1 M) 50 μl of cobalt chloride BCC (0.1 M) 5 μl of nickel(II) chloride BCC (1 M) 240 μl of polyethylene glycol 3350 BCC (50 w/v) 550 μl of water (100 v/v) |
F5 |
| pH 5.5 | 20 μl of ammonium acetate BCC (5 M) 100 μl of bis-tris chloride BCC pH 5.5 (1 M) 566 μl of polyethylene glycol 10000 BCC (30 w/v) 313 μl of water (100 v/v) |
F6 |
| pH 5.5 | 500 μl of polyethylene glycol 3350 BCC (50 w/v) 100 μl of bis-tris chloride BCC pH 5.5 (1 M) 57 μl of ammonium sulfate BCC (3.5 M) 342 μl of water (100 v/v) |
F7 |
| pH 6.5 | 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 57 μl of ammonium sulfate BCC (3.5 M) 342 μl of water (100 v/v) |
F8 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 57 μl of ammonium sulfate BCC (3.5 M) 342 μl of water (100 v/v) |
F9 |
| pH 8.5 | 78 μl of tris chloride BCC pH 9 (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 57 μl of ammonium sulfate BCC (3.5 M) 21 μl of tris chloride BCC pH 7 (1 M) 342 μl of water (100 v/v) |
F10 |
| pH 5.5 | 40 μl of sodium chloride BCC (5 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 100 μl of bis-tris chloride BCC pH 5.5 (1 M) 360 μl of water (100 v/v) |
F11 |
| pH 6.5 | 40 μl of sodium chloride BCC (5 M) 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 360 μl of water (100 v/v) |
F12 |
| pH 7.5 | 40 μl of sodium chloride BCC (5 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 360 μl of water (100 v/v) |
G1 |
| pH 8.5 | 40 μl of sodium chloride BCC (5 M) 78 μl of tris chloride BCC pH 9 (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 21 μl of tris chloride BCC pH 7 (1 M) 360 μl of water (100 v/v) |
G2 |
| pH 5.5 | 200 μl of lithium sulfate BCC (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 100 μl of bis-tris chloride BCC pH 5.5 (1 M) 200 μl of water (100 v/v) |
G3 |
| pH 6.5 | 200 μl of lithium sulfate BCC (1 M) 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 200 μl of water (100 v/v) |
G4 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 200 μl of lithium sulfate BCC (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 200 μl of water (100 v/v) |
G5 |
| pH 8.5 | 78 μl of tris chloride BCC pH 9 (1 M) 200 μl of lithium sulfate BCC (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 21 μl of tris chloride BCC pH 7 (1 M) 200 μl of water (100 v/v) |
G6 |
| pH 5.5 | 40 μl of ammonium acetate BCC (5 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 100 μl of bis-tris chloride BCC pH 5.5 (1 M) 360 μl of water (100 v/v) |
G7 |
| pH 6.5 | 40 μl of ammonium acetate BCC (5 M) 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 360 μl of water (100 v/v) |
G8 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 40 μl of ammonium acetate BCC (5 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 360 μl of water (100 v/v) |
G9 |
| pH 8.5 | 40 μl of ammonium acetate BCC (5 M) 78 μl of tris chloride BCC pH 9 (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 21 μl of tris chloride BCC pH 7 (1 M) 360 μl of water (100 v/v) |
G10 |
| pH 5.5 | 200 μl of magnesium chloride BCC (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 100 μl of bis-tris chloride BCC pH 5.5 (1 M) 200 μl of water (100 v/v) |
G11 |
| pH 6.5 | 200 μl of magnesium chloride BCC (1 M) 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 200 μl of water (100 v/v) |
G12 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 200 μl of magnesium chloride BCC (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 200 μl of water (100 v/v) |
H1 |
| pH 8.5 | 200 μl of magnesium chloride BCC (1 M) 78 μl of tris chloride BCC pH 9 (1 M) 500 μl of polyethylene glycol 3350 BCC (50 w/v) 21 μl of tris chloride BCC pH 7 (1 M) 200 μl of water (100 v/v) |
H2 |
| NA | 100 μl of potassium sodium tartrate BCC (2 M) 400 μl of polyethylene glycol 3350 BCC (50 w/v) 500 μl of water (100 v/v) |
H3 |
| pH 7 | 400 μl of polyethylene glycol 3350 BCC (50 w/v) 58 μl of sodium malonate-malonic acid BCC pH 7 (3.4 M) 541 μl of water (100 v/v) |
H4 |
| NA | 80 μl of triammonium citrate BCC (2.5 M) 400 μl of polyethylene glycol 3350 BCC (50 w/v) 520 μl of water (100 v/v) |
H5 |
| NA | 66 μl of sodium succinate BCC (1.5 M) 300 μl of polyethylene glycol 3350 BCC (50 w/v) 633 μl of water (100 v/v) |
H6 |
| NA | 400 μl of polyethylene glycol 3350 BCC (50 w/v) 50 μl of sodium formate BCC (4 M) 550 μl of water (100 v/v) |
H7 |
| pH 7 | 150 μl of DL-malic acid BCC pH 7 (1 M) 400 μl of polyethylene glycol 3350 BCC (50 w/v) 450 μl of water (100 v/v) |
H8 |
| NA | 100 μl of magnesium formate BCC (1 M) 300 μl of polyethylene glycol 3350 BCC (50 w/v) 600 μl of water (100 v/v) |
H9 |
| NA | 50 μl of zinc acetate BCC (1 M) 400 μl of polyethylene glycol 3350 BCC (50 w/v) 550 μl of water (100 v/v) |
H10 |
| NA | 125 μl of trisodium citrate BCC (1.6 M) 400 μl of polyethylene glycol 3350 BCC (50 w/v) 475 μl of water (100 v/v) |
H11 |
| NA | 600 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v) 50 μl of potassium thiocyanate BCC (2 M) 350 μl of water (100 v/v) |
H12 |
| NA | 150 μl of potassium bromide BCC (1 M) 600 μl of polyethylene glycol monomethyl ether 2000 BCC (50 w/v) 250 μl of water (100 v/v) |