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Supplemental Information

Screen Description

Anions

Qiagen / Anions
Created 2009-11-16.

Export

Anions

Barcodes

Anions

    Screen Structure

    Anions

    Well Count 96
    Number of distinct ingredients 18
    Internal diversity (score mean dist) 0.899

    Screen Composition

    More information about screen composition chart.
    2025-06-15T02:22:38.396901 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Anions
    Buffer 152
    Salt 128

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Anions Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Sodium acetate 0.1 M pH 4.6
    • Sodium acetate 2.5 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    833 μl of Sodium acetate (3 M)
    66 μl of water (100 v/v)
    A2
    • Sodium acetate 0.1 M pH 4.6
    • Sodium acetate 1.25 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    416 μl of Sodium acetate (3 M)
    483 μl of water (100 v/v)
    A3
    • Sodium acetate 0.1 M pH 4.6
    • tri-Sodium citrate 1.2 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    750 μl of tri-Sodium citrate (1.6 M)
    150 μl of water (100 v/v)
    A4
    • Sodium acetate 0.1 M pH 4.6
    • tri-Sodium citrate 0.6 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    375 μl of tri-Sodium citrate (1.6 M)
    525 μl of water (100 v/v)
    A5
    • Sodium acetate 0.1 M pH 4.6
    • Sodium fluoride 0.6 M -
    		pH 4.6
    750 μl of Sodium fluoride (0.8 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    150 μl of water (100 v/v)
    A6
    • Sodium acetate 0.1 M pH 4.6
    • Sodium fluoride 0.3 M -
    		pH 4.6
    375 μl of Sodium fluoride (0.8 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    525 μl of water (100 v/v)
    A7
    • MES 0.1 M pH 6.5
    • Sodium acetate 2.5 M -
    		pH 6.5
    833 μl of Sodium acetate (3 M)
    100 μl of MES pH 6.5 (1 M)
    66 μl of water (100 v/v)
    A8
    • MES 0.1 M pH 6.5
    • Sodium acetate 1.25 M -
    		pH 6.5
    416 μl of Sodium acetate (3 M)
    100 μl of MES pH 6.5 (1 M)
    483 μl of water (100 v/v)
    A9
    • MES 0.1 M pH 6.5
    • tri-Sodium citrate 1.2 M -
    		pH 6.5
    750 μl of tri-Sodium citrate (1.6 M)
    100 μl of MES pH 6.5 (1 M)
    150 μl of water (100 v/v)
    A10
    • MES 0.1 M pH 6.5
    • tri-Sodium citrate 0.6 M -
    		pH 6.5
    375 μl of tri-Sodium citrate (1.6 M)
    100 μl of MES pH 6.5 (1 M)
    525 μl of water (100 v/v)
    A11
    • MES 0.1 M pH 6.5
    • Sodium fluoride 0.6 M -
    		pH 6.5
    750 μl of Sodium fluoride (0.8 M)
    100 μl of MES pH 6.5 (1 M)
    150 μl of water (100 v/v)
    A12
    • MES 0.1 M pH 6.5
    • Sodium fluoride 0.3 M -
    		pH 6.5
    375 μl of Sodium fluoride (0.8 M)
    100 μl of MES pH 6.5 (1 M)
    525 μl of water (100 v/v)
    B1
    • HEPES 0.1 M pH 7.5
    • Sodium acetate 2.5 M -
    		pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    833 μl of Sodium acetate (3 M)
    66 μl of water (100 v/v)
    B2
    • HEPES 0.1 M pH 7.5
    • Sodium acetate 1.25 M -
    		pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    416 μl of Sodium acetate (3 M)
    483 μl of water (100 v/v)
    B3
    • HEPES 0.1 M pH 7.5
    • tri-Sodium citrate 1.2 M -
    		pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    750 μl of tri-Sodium citrate (1.6 M)
    150 μl of water (100 v/v)
    B4
    • HEPES 0.1 M pH 7.5
    • tri-Sodium citrate 0.6 M -
    		pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    375 μl of tri-Sodium citrate (1.6 M)
    525 μl of water (100 v/v)
    B5
    • HEPES 0.1 M pH 7.5
    • Sodium fluoride 0.6 M -
    		pH 7.5
    750 μl of Sodium fluoride (0.8 M)
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    150 μl of water (100 v/v)
    B6
    • HEPES 0.1 M pH 7.5
    • Sodium fluoride 0.3 M -
    		pH 7.5
    375 μl of Sodium fluoride (0.8 M)
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    525 μl of water (100 v/v)
    B7
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium acetate 2.5 M -
    		pH 8.5
    833 μl of Sodium acetate (3 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    66 μl of water (100 v/v)
    B8
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium acetate 1.25 M -
    		pH 8.5
    416 μl of Sodium acetate (3 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    483 μl of water (100 v/v)
    B9
    • tris chloride BCC 0.1 M pH 8.5
    • tri-Sodium citrate 1.2 M -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    750 μl of tri-Sodium citrate (1.6 M)
    150 μl of water (100 v/v)
    B10
    • tris chloride BCC 0.1 M pH 8.5
    • tri-Sodium citrate 0.6 M -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    375 μl of tri-Sodium citrate (1.6 M)
    525 μl of water (100 v/v)
    B11
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium fluoride 0.6 M -
    		pH 8.5
    750 μl of Sodium fluoride (0.8 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    150 μl of water (100 v/v)
    B12
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium fluoride 0.3 M -
    		pH 8.5
    375 μl of Sodium fluoride (0.8 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    525 μl of water (100 v/v)
    C1
    • Sodium acetate 0.1 M pH 4.6
    • Sodium formate 3.5 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    500 μl of Sodium formate (7 M)
    400 μl of water (100 v/v)
    C2
    • Sodium acetate 0.1 M pH 4.6
    • Sodium formate 1.75 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    250 μl of Sodium formate (7 M)
    650 μl of water (100 v/v)
    C3
    • Sodium acetate 0.1 M pH 4.6
    • Sodium bromide 3.5 M -
    		pH 4.6
    700 μl of Sodium bromide (5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    200 μl of water (100 v/v)
    C4
    • Sodium acetate 0.1 M pH 4.6
    • Sodium bromide 1.75 M -
    		pH 4.6
    350 μl of Sodium bromide (5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    550 μl of water (100 v/v)
    C5
    • Sodium acetate 0.1 M pH 4.6
    • di-Sodium malonate 2.4 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    705 μl of di-Sodium malonate pH 7 (3.4 M)
    194 μl of water (100 v/v)
    C6
    • Sodium acetate 0.1 M pH 4.6
    • di-Sodium malonate 1.2 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    352 μl of di-Sodium malonate pH 7 (3.4 M)
    547 μl of water (100 v/v)
    C7
    • MES 0.1 M pH 6.5
    • Sodium formate 3.5 M -
    		pH 6.5
    500 μl of Sodium formate (7 M)
    100 μl of MES pH 6.5 (1 M)
    400 μl of water (100 v/v)
    C8
    • MES 0.1 M pH 6.5
    • Sodium formate 1.75 M -
    		pH 6.5
    250 μl of Sodium formate (7 M)
    100 μl of MES pH 6.5 (1 M)
    650 μl of water (100 v/v)
    C9
    • MES 0.1 M pH 6.5
    • Sodium bromide 3.5 M -
    		pH 6.5
    700 μl of Sodium bromide (5 M)
    100 μl of MES pH 6.5 (1 M)
    200 μl of water (100 v/v)
    C10
    • MES 0.1 M pH 6.5
    • Sodium bromide 1.75 M -
    		pH 6.5
    350 μl of Sodium bromide (5 M)
    100 μl of MES pH 6.5 (1 M)
    550 μl of water (100 v/v)
    C11
    • MES 0.1 M pH 6.5
    • di-Sodium malonate 2.4 M -
    		pH 6.5
    705 μl of di-Sodium malonate pH 7 (3.4 M)
    100 μl of MES pH 6.5 (1 M)
    194 μl of water (100 v/v)
    C12
    • MES 0.1 M pH 6.5
    • di-Sodium malonate 1.2 M -
    		pH 6.5
    352 μl of di-Sodium malonate pH 7 (3.4 M)
    100 μl of MES pH 6.5 (1 M)
    547 μl of water (100 v/v)
    D1
    • HEPES 0.1 M pH 7.5
    • Sodium formate 3.5 M -
    		pH 7.5
    500 μl of Sodium formate (7 M)
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    400 μl of water (100 v/v)
    D2
    • HEPES 0.1 M pH 7.5
    • Sodium formate 1.75 M -
    		pH 7.5
    250 μl of Sodium formate (7 M)
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    650 μl of water (100 v/v)
    D3
    • HEPES 0.1 M pH 7.5
    • Sodium bromide 3.5 M -
    		pH 7.5
    700 μl of Sodium bromide (5 M)
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    200 μl of water (100 v/v)
    D4
    • HEPES 0.1 M pH 7.5
    • Sodium bromide 1.75 M -
    		pH 7.5
    350 μl of Sodium bromide (5 M)
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    550 μl of water (100 v/v)
    D5
    • HEPES 0.1 M pH 7.5
    • di-Sodium malonate 2.4 M -
    		pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    705 μl of di-Sodium malonate pH 7 (3.4 M)
    61 μl of HEPES pH 8 (1 M)
    194 μl of water (100 v/v)
    D6
    • HEPES 0.1 M pH 7.5
    • di-Sodium malonate 1.2 M -
    		pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    352 μl of di-Sodium malonate pH 7 (3.4 M)
    61 μl of HEPES pH 8 (1 M)
    547 μl of water (100 v/v)
    D7
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium formate 3.5 M -
    		pH 8.5
    500 μl of Sodium formate (7 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of water (100 v/v)
    D8
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium formate 1.75 M -
    		pH 8.5
    250 μl of Sodium formate (7 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    650 μl of water (100 v/v)
    D9
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium bromide 3.5 M -
    		pH 8.5
    700 μl of Sodium bromide (5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    200 μl of water (100 v/v)
    D10
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium bromide 1.75 M -
    		pH 8.5
    350 μl of Sodium bromide (5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    550 μl of water (100 v/v)
    D11
    • tris chloride BCC 0.1 M pH 8.5
    • di-Sodium malonate 2.4 M -
    		pH 8.5
    705 μl of di-Sodium malonate pH 7 (3.4 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    194 μl of water (100 v/v)
    D12
    • tris chloride BCC 0.1 M pH 8.5
    • di-Sodium malonate 1.2 M -
    		pH 8.5
    352 μl of di-Sodium malonate pH 7 (3.4 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    547 μl of water (100 v/v)
    E1
    • Sodium acetate 0.1 M pH 4.6
    • Sodium nitrate 3.5 M -
    		pH 4.6
    500 μl of Sodium nitrate (7 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    400 μl of water (100 v/v)
    E2
    • Sodium acetate 0.1 M pH 4.6
    • Sodium nitrate 1.75 M -
    		pH 4.6
    250 μl of Sodium nitrate (7 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    650 μl of water (100 v/v)
    E3
    • Sodium acetate 0.1 M pH 4.6
    • Potassium di-hydrogen phosphate 0.9 M -
    • Sodium di-hydrogen phosphate 0.9 M -
    		pH 4.6
    225 μl of Potassium di-hydrogen phosphate (4 M)
    300 μl of Sodium di-hydrogen phosphate (3 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    375 μl of water (100 v/v)
    E4
    • Sodium acetate 0.1 M pH 4.6
    • Potassium di-hydrogen phosphate 0.45 M -
    • Sodium di-hydrogen phosphate 0.45 M -
    		pH 4.6
    112 μl of Potassium di-hydrogen phosphate (4 M)
    150 μl of Sodium di-hydrogen phosphate (3 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    637 μl of water (100 v/v)
    E5
    • Sodium acetate 0.1 M pH 4.6
    • di-Sodium succinate 1 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    833 μl of di-Sodium succinate (1.2 M)
    66 μl of water (100 v/v)
    E6
    • Sodium acetate 0.1 M pH 4.6
    • di-Sodium succinate 0.5 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    416 μl of di-Sodium succinate (1.2 M)
    483 μl of water (100 v/v)
    E7
    • MES 0.1 M pH 6.5
    • Sodium nitrate 3.5 M -
    		pH 6.5
    500 μl of Sodium nitrate (7 M)
    100 μl of MES pH 6.5 (1 M)
    400 μl of water (100 v/v)
    E8
    • MES 0.1 M pH 6.5
    • Sodium nitrate 1.75 M -
    		pH 6.5
    250 μl of Sodium nitrate (7 M)
    100 μl of MES pH 6.5 (1 M)
    650 μl of water (100 v/v)
    E9
    • MES 0.1 M pH 6.5
    • Potassium di-hydrogen phosphate 0.9 M -
    • Sodium di-hydrogen phosphate 0.9 M -
    		pH 6.5
    225 μl of Potassium di-hydrogen phosphate (4 M)
    300 μl of Sodium di-hydrogen phosphate (3 M)
    100 μl of MES pH 6.5 (1 M)
    375 μl of water (100 v/v)
    E10
    • MES 0.1 M pH 6.5
    • Potassium di-hydrogen phosphate 0.45 M -
    • Sodium di-hydrogen phosphate 0.45 M -
    		pH 6.5
    112 μl of Potassium di-hydrogen phosphate (4 M)
    150 μl of Sodium di-hydrogen phosphate (3 M)
    100 μl of MES pH 6.5 (1 M)
    637 μl of water (100 v/v)
    E11
    • MES 0.1 M pH 6.5
    • di-Sodium succinate 1 M -
    		pH 6.5
    833 μl of di-Sodium succinate (1.2 M)
    100 μl of MES pH 6.5 (1 M)
    66 μl of water (100 v/v)
    E12
    • MES 0.1 M pH 6.5
    • di-Sodium succinate 0.5 M -
    		pH 6.5
    416 μl of di-Sodium succinate (1.2 M)
    100 μl of MES pH 6.5 (1 M)
    483 μl of water (100 v/v)
    F1
    • HEPES 0.1 M pH 7.5
    • Sodium nitrate 3.5 M -
    		pH 7.5
    500 μl of Sodium nitrate (7 M)
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    400 μl of water (100 v/v)
    F2
    • HEPES 0.1 M pH 7.5
    • Sodium nitrate 1.75 M -
    		pH 7.5
    250 μl of Sodium nitrate (7 M)
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    650 μl of water (100 v/v)
    F3
    • HEPES 0.1 M pH 7.5
    • Potassium di-hydrogen phosphate 0.9 M -
    • Sodium di-hydrogen phosphate 0.9 M -
    		pH 7.5
    225 μl of Potassium di-hydrogen phosphate (4 M)
    300 μl of Sodium di-hydrogen phosphate (3 M)
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    375 μl of water (100 v/v)
    F4
    • HEPES 0.1 M pH 7.5
    • Potassium di-hydrogen phosphate 0.45 M -
    • Sodium di-hydrogen phosphate 0.45 M -
    		pH 7.5
    112 μl of Potassium di-hydrogen phosphate (4 M)
    150 μl of Sodium di-hydrogen phosphate (3 M)
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    637 μl of water (100 v/v)
    F5
    • HEPES 0.1 M pH 7.5
    • di-Sodium succinate 1 M -
    		pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    833 μl of di-Sodium succinate (1.2 M)
    66 μl of water (100 v/v)
    F6
    • HEPES 0.1 M pH 7.5
    • di-Sodium succinate 0.5 M -
    		pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    416 μl of di-Sodium succinate (1.2 M)
    483 μl of water (100 v/v)
    F7
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium nitrate 3.5 M -
    		pH 8.5
    500 μl of Sodium nitrate (7 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of water (100 v/v)
    F8
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium nitrate 1.75 M -
    		pH 8.5
    250 μl of Sodium nitrate (7 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    650 μl of water (100 v/v)
    F9
    • tris chloride BCC 0.1 M pH 8.5
    • Potassium di-hydrogen phosphate 0.9 M -
    • Sodium di-hydrogen phosphate 0.9 M -
    		pH 8.5
    225 μl of Potassium di-hydrogen phosphate (4 M)
    300 μl of Sodium di-hydrogen phosphate (3 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    375 μl of water (100 v/v)
    F10
    • tris chloride BCC 0.1 M pH 8.5
    • Potassium di-hydrogen phosphate 0.45 M -
    • Sodium di-hydrogen phosphate 0.45 M -
    		pH 8.5
    112 μl of Potassium di-hydrogen phosphate (4 M)
    150 μl of Sodium di-hydrogen phosphate (3 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    637 μl of water (100 v/v)
    F11
    • tris chloride BCC 0.1 M pH 8.5
    • di-Sodium succinate 1 M -
    		pH 8.5
    833 μl of di-Sodium succinate (1.2 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    66 μl of water (100 v/v)
    F12
    • tris chloride BCC 0.1 M pH 8.5
    • di-Sodium succinate 0.5 M -
    		pH 8.5
    416 μl of di-Sodium succinate (1.2 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    483 μl of water (100 v/v)
    G1
    • Sodium acetate 0.1 M pH 4.6
    • Sodium sulfate 0.75 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    750 μl of Sodium sulfate (1 M)
    150 μl of water (100 v/v)
    G2
    • Sodium acetate 0.1 M pH 4.6
    • Sodium sulfate 0.37 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    370 μl of Sodium sulfate (1 M)
    530 μl of water (100 v/v)
    G3
    • di-Sodium tartrate 0.1 M pH 5.6
    • Potassium Sodium tartrate 1.2 M -
    		pH 5.6
    100 μl of di-Sodium tartrate pH 5.6 (1 M)
    600 μl of Potassium Sodium tartrate (2 M)
    300 μl of water (100 v/v)
    G4
    • di-Sodium tartrate 0.1 M pH 5.6
    • Potassium Sodium tartrate 0.6 M -
    		pH 5.6
    100 μl of di-Sodium tartrate pH 5.6 (1 M)
    300 μl of Potassium Sodium tartrate (2 M)
    600 μl of water (100 v/v)
    G5
    • Sodium acetate 0.1 M pH 4.6
    • Sodium thiocyanate 2.4 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    300 μl of Sodium thiocyanate (8 M)
    600 μl of water (100 v/v)
    G6
    • Sodium acetate 0.1 M pH 4.6
    • Sodium thiocyanate 1.2 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    150 μl of Sodium thiocyanate (8 M)
    750 μl of water (100 v/v)
    G7
    • MES 0.1 M pH 6.5
    • Sodium sulfate 0.75 M -
    		pH 6.5
    750 μl of Sodium sulfate (1 M)
    100 μl of MES pH 6.5 (1 M)
    150 μl of water (100 v/v)
    G8
    • MES 0.1 M pH 6.5
    • Sodium sulfate 0.37 M -
    		pH 6.5
    370 μl of Sodium sulfate (1 M)
    100 μl of MES pH 6.5 (1 M)
    530 μl of water (100 v/v)
    G9
    • MES 0.1 M pH 6.5
    • Potassium Sodium tartrate 1.2 M -
    		pH 6.5
    600 μl of Potassium Sodium tartrate (2 M)
    100 μl of MES pH 6.5 (1 M)
    300 μl of water (100 v/v)
    G10
    • MES 0.1 M pH 6.5
    • Potassium Sodium tartrate 0.6 M -
    		pH 6.5
    300 μl of Potassium Sodium tartrate (2 M)
    100 μl of MES pH 6.5 (1 M)
    600 μl of water (100 v/v)
    G11
    • MES 0.1 M pH 6.5
    • Sodium thiocyanate 2.4 M -
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    300 μl of Sodium thiocyanate (8 M)
    600 μl of water (100 v/v)
    G12
    • MES 0.1 M pH 6.5
    • Sodium thiocyanate 1.2 M -
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    150 μl of Sodium thiocyanate (8 M)
    750 μl of water (100 v/v)
    H1
    • HEPES 0.1 M pH 7.5
    • Sodium sulfate 0.75 M -
    		pH 7.5
    750 μl of Sodium sulfate (1 M)
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    150 μl of water (100 v/v)
    H2
    • HEPES 0.1 M pH 7.5
    • Sodium sulfate 0.37 M -
    		pH 7.5
    370 μl of Sodium sulfate (1 M)
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    530 μl of water (100 v/v)
    H3
    • HEPES 0.1 M pH 7.5
    • Potassium Sodium tartrate 1.2 M -
    		pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    600 μl of Potassium Sodium tartrate (2 M)
    300 μl of water (100 v/v)
    H4
    • HEPES 0.1 M pH 7.5
    • Potassium Sodium tartrate 0.6 M -
    		pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    300 μl of Potassium Sodium tartrate (2 M)
    600 μl of water (100 v/v)
    H5
    • HEPES 0.1 M pH 7.5
    • Sodium thiocyanate 2.4 M -
    		pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    300 μl of Sodium thiocyanate (8 M)
    600 μl of water (100 v/v)
    H6
    • HEPES 0.1 M pH 7.5
    • Sodium thiocyanate 1.2 M -
    		pH 7.5
    38 μl of HEPES pH 6.5 (1 M)
    61 μl of HEPES pH 8 (1 M)
    150 μl of Sodium thiocyanate (8 M)
    750 μl of water (100 v/v)
    H7
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium sulfate 0.75 M -
    		pH 8.5
    750 μl of Sodium sulfate (1 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    150 μl of water (100 v/v)
    H8
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium sulfate 0.37 M -
    		pH 8.5
    370 μl of Sodium sulfate (1 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    530 μl of water (100 v/v)
    H9
    • tris chloride BCC 0.1 M pH 8.5
    • Potassium Sodium tartrate 1.2 M -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    600 μl of Potassium Sodium tartrate (2 M)
    300 μl of water (100 v/v)
    H10
    • tris chloride BCC 0.1 M pH 8.5
    • Potassium Sodium tartrate 0.6 M -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    300 μl of Potassium Sodium tartrate (2 M)
    600 μl of water (100 v/v)
    H11
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium thiocyanate 2.4 M -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    300 μl of Sodium thiocyanate (8 M)
    600 μl of water (100 v/v)
    H12
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium thiocyanate 1.2 M -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    150 μl of Sodium thiocyanate (8 M)
    750 μl of water (100 v/v)